LOCUS       DACEKQ010000001        82422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEKQ010000001 DACEKQ010000000
VERSION     DACEKQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.001"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(440..2821)
                     /locus_tag="IW671_000001"
     CDS             complement(440..2821)
                     /locus_tag="IW671_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0897024.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2811..3458)
                     /gene="recU"
                     /locus_tag="IW671_000002"
     CDS             complement(2811..3458)
                     /gene="recU"
                     /locus_tag="IW671_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0897025.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3522..4061
                     /locus_tag="IW671_000003"
     CDS             3522..4061
                     /locus_tag="IW671_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0897026.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4151..4570
                     /gene="gpsB"
                     /locus_tag="IW671_000004"
     CDS             4151..4570
                     /gene="gpsB"
                     /locus_tag="IW671_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0897027.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4592..4959
                     /gene="rnpB"
                     /locus_tag="IW671_000005"
     ncRNA           4592..4959
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW671_000005"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5168..6334
                     /locus_tag="IW671_000006"
     CDS             5168..6334
                     /locus_tag="IW671_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0897028.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6380..7876
                     /locus_tag="IW671_000007"
     CDS             6380..7876
                     /locus_tag="IW671_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0897029.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7957..8640
                     /locus_tag="IW671_000008"
     CDS             7957..8640
                     /locus_tag="IW671_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0897030.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9285..10475
                     /locus_tag="IW671_000009"
     CDS             9285..10475
                     /locus_tag="IW671_000009"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0897031.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10577..11530
                     /locus_tag="IW671_000010"
     CDS             10577..11530
                     /locus_tag="IW671_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0897032.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11599..12948
                     /locus_tag="IW671_000011"
     CDS             11599..12948
                     /locus_tag="IW671_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897033.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13131..13454)
                     /locus_tag="IW671_000012"
     CDS             complement(13131..13454)
                     /locus_tag="IW671_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897034.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13583..14746
                     /locus_tag="IW671_000013"
     CDS             13583..14746
                     /locus_tag="IW671_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897035.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14816..16165
                     /locus_tag="IW671_000014"
     CDS             14816..16165
                     /locus_tag="IW671_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0897036.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16210..16641)
                     /locus_tag="IW671_000015"
     CDS             complement(16210..16641)
                     /locus_tag="IW671_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897037.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17029..19671
                     /gene="alaS"
                     /locus_tag="IW671_000016"
     CDS             17029..19671
                     /gene="alaS"
                     /locus_tag="IW671_000016"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0897038.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19871..20467
                     /locus_tag="IW671_000017"
     CDS             19871..20467
                     /locus_tag="IW671_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0897039.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20589..21737
                     /locus_tag="IW671_000018"
     CDS             20589..21737
                     /locus_tag="IW671_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0897040.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21775..22188)
                     /locus_tag="IW671_000019"
     CDS             complement(21775..22188)
                     /locus_tag="IW671_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0897041.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22267..22698
                     /locus_tag="IW671_000020"
     CDS             22267..22698
                     /locus_tag="IW671_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0897042.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22797..23030)
                     /locus_tag="IW671_000021"
     CDS             complement(22797..23030)
                     /locus_tag="IW671_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0897043.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23213..24883
                     /locus_tag="IW671_000022"
     CDS             23213..24883
                     /locus_tag="IW671_000022"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0897044.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25030..25416
                     /locus_tag="IW671_000023"
     CDS             25030..25416
                     /locus_tag="IW671_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897045.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25403..26101
                     /locus_tag="IW671_000024"
     CDS             25403..26101
                     /locus_tag="IW671_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0897046.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26106..26570
                     /locus_tag="IW671_000025"
     CDS             26106..26570
                     /locus_tag="IW671_000025"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0897047.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26567..27478
                     /locus_tag="IW671_000026"
     CDS             26567..27478
                     /locus_tag="IW671_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0897048.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27713..28252
                     /gene="pyrR"
                     /locus_tag="IW671_000027"
     CDS             27713..28252
                     /gene="pyrR"
                     /locus_tag="IW671_000027"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0897049.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28266..29549
                     /locus_tag="IW671_000028"
     CDS             28266..29549
                     /locus_tag="IW671_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0897050.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29562..30488
                     /locus_tag="IW671_000029"
     CDS             29562..30488
                     /locus_tag="IW671_000029"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0897051.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30507..31787
                     /locus_tag="IW671_000030"
     CDS             30507..31787
                     /locus_tag="IW671_000030"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0897052.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31792..32868
                     /gene="carA"
                     /locus_tag="IW671_000031"
     CDS             31792..32868
                     /gene="carA"
                     /locus_tag="IW671_000031"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0897053.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32873..36058
                     /gene="carB"
                     /locus_tag="IW671_000032"
     CDS             32873..36058
                     /gene="carB"
                     /locus_tag="IW671_000032"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0897054.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36112..36888
                     /locus_tag="IW671_000033"
     CDS             36112..36888
                     /locus_tag="IW671_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0897055.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36885..37817
                     /locus_tag="IW671_000034"
     CDS             36885..37817
                     /locus_tag="IW671_000034"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0897056.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37814..38530
                     /gene="pyrF"
                     /locus_tag="IW671_000035"
     CDS             37814..38530
                     /gene="pyrF"
                     /locus_tag="IW671_000035"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0897057.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38537..39166
                     /locus_tag="IW671_000036"
     CDS             38537..39166
                     /locus_tag="IW671_000036"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0897058.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39154..39468
                     /locus_tag="IW671_000037"
     CDS             39154..39468
                     /locus_tag="IW671_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897059.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39506..40147
                     /locus_tag="IW671_000038"
     CDS             39506..40147
                     /locus_tag="IW671_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0897060.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40178..40882
                     /locus_tag="IW671_000039"
     CDS             40178..40882
                     /locus_tag="IW671_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0897061.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40879..41781
                     /locus_tag="IW671_000040"
     CDS             40879..41781
                     /locus_tag="IW671_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0897062.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41939..43645)
                     /locus_tag="IW671_000041"
     CDS             complement(41939..43645)
                     /locus_tag="IW671_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0897063.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43835..44458
                     /locus_tag="IW671_000042"
     CDS             43835..44458
                     /locus_tag="IW671_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897064.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44908..45957
                     /locus_tag="IW671_000043"
     CDS             44908..45957
                     /locus_tag="IW671_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897065.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45954..46835
                     /locus_tag="IW671_000044"
     CDS             45954..46835
                     /locus_tag="IW671_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897066.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46838..47608
                     /locus_tag="IW671_000045"
     CDS             46838..47608
                     /locus_tag="IW671_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0897067.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47887..48741
                     /gene="aroE"
                     /locus_tag="IW671_000046"
     CDS             47887..48741
                     /gene="aroE"
                     /locus_tag="IW671_000046"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0897068.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48919..50082
                     /locus_tag="IW671_000047"
     CDS             48919..50082
                     /locus_tag="IW671_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0897069.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50199..50609
                     /locus_tag="IW671_000048"
     CDS             50199..50609
                     /locus_tag="IW671_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0897070.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50783..53011
                     /gene="topB"
                     /locus_tag="IW671_000049"
     CDS             50783..53011
                     /gene="topB"
                     /locus_tag="IW671_000049"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0897071.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53149..53466
                     /locus_tag="IW671_000050"
     CDS             53149..53466
                     /locus_tag="IW671_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897072.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53626..53862
                     /locus_tag="IW671_000051"
     CDS             53626..53862
                     /locus_tag="IW671_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0897073.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53859..54257
                     /locus_tag="IW671_000052"
     CDS             53859..54257
                     /locus_tag="IW671_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0897074.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(54924..55868)
                     /locus_tag="IW671_000053"
     CDS             complement(54924..55868)
                     /locus_tag="IW671_000053"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0897075.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(56164..57108)
                     /locus_tag="IW671_000054"
     CDS             complement(56164..57108)
                     /locus_tag="IW671_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897076.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            57329..58543
                     /locus_tag="IW671_000055"
     CDS             57329..58543
                     /locus_tag="IW671_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0897077.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            58565..60580
                     /locus_tag="IW671_000056"
     CDS             58565..60580
                     /locus_tag="IW671_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0897078.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(60663..62036)
                     /locus_tag="IW671_000057"
     CDS             complement(60663..62036)
                     /locus_tag="IW671_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0897079.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(62062..62346)
                     /locus_tag="IW671_000058"
     CDS             complement(62062..62346)
                     /locus_tag="IW671_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897080.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(62348..64363)
                     /locus_tag="IW671_000059"
     CDS             complement(62348..64363)
                     /locus_tag="IW671_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0897081.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(64542..64847)
                     /locus_tag="IW671_000060"
     CDS             complement(64542..64847)
                     /locus_tag="IW671_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0897082.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(64936..65967)
                     /locus_tag="IW671_000061"
     CDS             complement(64936..65967)
                     /locus_tag="IW671_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0897083.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            66174..67049
                     /locus_tag="IW671_000062"
     CDS             66174..67049
                     /locus_tag="IW671_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0897084.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(67201..68043)
                     /locus_tag="IW671_000063"
     CDS             complement(67201..68043)
                     /locus_tag="IW671_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0897085.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(68135..70012)
                     /locus_tag="IW671_000064"
     CDS             complement(68135..70012)
                     /locus_tag="IW671_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0897086.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(70009..71424)
                     /locus_tag="IW671_000065"
     CDS             complement(70009..71424)
                     /locus_tag="IW671_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0897087.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETEMVQITLHKLLFPNGQL
                     PKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGASS
                     GMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVVLP
                     VHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVNIL
                     DYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKYAG
                     KKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPDKR
                     LAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLKEI
                     EAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLGVV
                     GMVFASFAIWLFIKKRTGVKK"
     gene            complement(71427..74816)
                     /locus_tag="IW671_000066"
     CDS             complement(71427..74816)
                     /locus_tag="IW671_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0897088.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            75073..76465
                     /locus_tag="IW671_000067"
                     /pseudo
     CDS             75073..76465
                     /locus_tag="IW671_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(76538..77185)
                     /locus_tag="IW671_000068"
     CDS             complement(76538..77185)
                     /locus_tag="IW671_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0897089.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(77193..77879)
                     /locus_tag="IW671_000069"
     CDS             complement(77193..77879)
                     /locus_tag="IW671_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897090.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(77885..78958)
                     /locus_tag="IW671_000070"
     CDS             complement(77885..78958)
                     /locus_tag="IW671_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0897091.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            79180..79854
                     /locus_tag="IW671_000071"
     CDS             79180..79854
                     /locus_tag="IW671_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897092.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            79859..81199
                     /locus_tag="IW671_000072"
     CDS             79859..81199
                     /locus_tag="IW671_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0897093.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(81348..82334)
                     /locus_tag="IW671_000073"
     CDS             complement(81348..82334)
                     /locus_tag="IW671_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0897094.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        25934 a        14758 c        16817 g        24913 t
ORIGIN      
        1 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
       61 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
      121 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
      181 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
      241 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
      301 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
      361 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
      421 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
      481 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
      541 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
      601 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
      661 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
      721 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
      781 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
      841 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
      901 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
      961 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     1021 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     1081 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     1141 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     1201 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     1261 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     1321 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     1381 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     1441 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     1501 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     1561 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     1621 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     1681 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     1741 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     1801 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     1861 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     1921 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     1981 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     2041 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     2101 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     2161 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     2221 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     2281 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     2341 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     2401 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     2461 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     2521 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     2581 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     2641 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     2701 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     2761 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     2821 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     2881 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     2941 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     3001 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     3061 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     3121 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     3181 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     3241 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     3301 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     3361 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     3421 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     3481 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     3541 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     3601 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     3661 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     3721 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     3781 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     3841 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     3901 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     3961 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     4021 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     4081 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     4141 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     4201 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     4261 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     4321 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     4381 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     4441 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     4501 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     4561 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     4621 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     4681 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     4741 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     4801 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     4861 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     4921 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     4981 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     5041 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     5101 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     5161 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     5221 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     5281 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     5341 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     5401 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     5461 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     5521 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     5581 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     5641 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     5701 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     5761 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     5821 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     5881 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     5941 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     6001 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     6061 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
     6121 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
     6181 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
     6241 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
     6301 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
     6361 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
     6421 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
     6481 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
     6541 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
     6601 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
     6661 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
     6721 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
     6781 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
     6841 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
     6901 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
     6961 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
     7021 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
     7081 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
     7141 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
     7201 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
     7261 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
     7321 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
     7381 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
     7441 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
     7501 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
     7561 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
     7621 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
     7681 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
     7741 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
     7801 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
     7861 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
     7921 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
     7981 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
     8041 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
     8101 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
     8161 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
     8221 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
     8281 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
     8341 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
     8401 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
     8461 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
     8521 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
     8581 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
     8641 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
     8701 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
     8761 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
     8821 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
     8881 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
     8941 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
     9001 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
     9061 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
     9121 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
     9181 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
     9241 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
     9301 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
     9361 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
     9421 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
     9481 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
     9541 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
     9601 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
     9661 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
     9721 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
     9781 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
     9841 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
     9901 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
     9961 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    10021 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    10081 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    10141 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    10201 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    10261 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    10321 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    10381 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    10441 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    10501 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    10561 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    10621 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    10681 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    10741 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    10801 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    10861 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    10921 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    10981 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    11041 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    11101 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    11161 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    11221 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    11281 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    11341 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    11401 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    11461 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    11521 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    11581 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    11641 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    11701 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    11761 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    11821 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    11881 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    11941 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    12001 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    12061 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    12121 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    12181 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    12241 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    12301 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    12361 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    12421 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    12481 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    12541 cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    12601 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    12661 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    12721 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    12781 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    12841 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    12901 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    12961 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    13021 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    13081 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    13141 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    13201 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    13261 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    13321 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    13381 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    13441 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    13501 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    13561 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    13621 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    13681 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    13741 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    13801 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    13861 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    13921 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    13981 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    14041 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    14101 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    14161 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    14221 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    14281 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    14341 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    14401 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    14461 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    14521 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    14581 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    14641 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    14701 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    14761 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    14821 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    14881 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    14941 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    15001 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    15061 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    15121 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    15181 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    15241 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    15301 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    15361 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    15421 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    15481 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    15541 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    15601 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    15661 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    15721 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    15781 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    15841 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    15901 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    15961 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    16021 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    16081 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    16141 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    16201 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    16261 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    16321 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    16381 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    16441 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    16501 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    16561 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    16621 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    16681 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    16741 attgagatat tgacagtgat caatagaatg cgtatactaa ataagtacga aaaggatatg
    16801 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    16861 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    16921 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    16981 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    17041 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    17101 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    17161 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    17221 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    17281 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    17341 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    17401 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    17461 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    17521 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    17581 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    17641 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    17701 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    17761 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    17821 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    17881 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    17941 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    18001 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    18061 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    18121 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    18181 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    18241 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    18301 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    18361 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    18421 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    18481 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    18541 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    18601 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    18661 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    18721 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    18781 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    18841 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    18901 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    18961 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    19021 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    19081 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    19141 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    19201 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    19261 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    19321 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    19381 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    19441 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    19501 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    19561 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    19621 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    19681 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    19741 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    19801 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    19861 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    19921 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    19981 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    20041 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    20101 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    20161 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    20221 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    20281 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    20341 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    20401 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    20461 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    20521 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    20581 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    20641 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    20701 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    20761 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    20821 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    20881 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    20941 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    21001 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    21061 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    21121 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    21181 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    21241 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    21301 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    21361 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    21421 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    21481 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    21541 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    21601 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    21661 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    21721 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    21781 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    21841 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    21901 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    21961 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    22021 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    22081 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    22141 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    22201 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    22261 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    22321 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    22381 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    22441 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    22501 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    22561 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    22621 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    22681 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    22741 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    22801 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    22861 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    22921 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    22981 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    23041 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    23101 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    23161 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    23221 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    23281 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    23341 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    23401 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    23461 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    23521 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    23581 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    23641 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    23701 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    23761 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    23821 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    23881 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    23941 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    24001 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    24061 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    24121 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    24181 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    24241 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    24301 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    24361 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    24421 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    24481 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    24541 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    24601 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    24661 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    24721 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    24781 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    24841 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    24901 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    24961 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    25021 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    25081 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    25141 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    25201 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    25261 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    25321 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    25381 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    25441 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    25501 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    25561 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    25621 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    25681 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    25741 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    25801 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    25861 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    25921 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    25981 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    26041 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    26101 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    26161 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    26221 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    26281 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    26341 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    26401 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    26461 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    26521 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    26581 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    26641 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    26701 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    26761 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    26821 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    26881 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    26941 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    27001 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    27061 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    27121 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    27181 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    27241 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    27301 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    27361 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    27421 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    27481 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    27541 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    27601 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    27661 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    27721 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    27781 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    27841 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    27901 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    27961 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    28021 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    28081 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    28141 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    28201 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    28261 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    28321 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    28381 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    28441 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    28501 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    28561 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    28621 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    28681 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    28741 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    28801 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    28861 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    28921 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    28981 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    29041 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    29101 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    29161 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    29221 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    29281 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    29341 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    29401 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    29461 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    29521 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    29581 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    29641 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    29701 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    29761 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    29821 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    29881 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    29941 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    30001 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    30061 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    30121 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    30181 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    30241 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    30301 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    30361 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    30421 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    30481 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    30541 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    30601 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    30661 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    30721 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    30781 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    30841 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    30901 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    30961 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    31021 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    31081 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    31141 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    31201 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    31261 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    31321 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    31381 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    31441 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    31501 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    31561 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    31621 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    31681 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    31741 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    31801 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    31861 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    31921 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    31981 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    32041 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    32101 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    32161 ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc atttgatcag
    32221 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    32281 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    32341 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    32401 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    32461 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    32521 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    32581 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    32641 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    32701 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    32761 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    32821 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    32881 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    32941 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    33001 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    33061 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    33121 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    33181 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    33241 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    33301 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    33361 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    33421 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    33481 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    33541 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    33601 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    33661 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    33721 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    33781 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    33841 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    33901 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    33961 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    34021 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    34081 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    34141 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    34201 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    34261 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    34321 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    34381 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    34441 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    34501 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    34561 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    34621 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    34681 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    34741 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    34801 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    34861 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    34921 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    34981 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    35041 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    35101 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    35161 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    35221 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    35281 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    35341 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    35401 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    35461 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    35521 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    35581 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    35641 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    35701 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    35761 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    35821 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    35881 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    35941 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    36001 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    36061 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    36121 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    36181 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    36241 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    36301 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    36361 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    36421 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    36481 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    36541 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    36601 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    36661 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    36721 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    36781 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    36841 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    36901 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    36961 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    37021 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    37081 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    37141 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    37201 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    37261 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    37321 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    37381 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    37441 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    37501 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    37561 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    37621 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    37681 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    37741 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    37801 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    37861 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    37921 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    37981 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    38041 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    38101 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    38161 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    38221 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    38281 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    38341 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    38401 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    38461 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    38521 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    38581 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    38641 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    38701 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    38761 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    38821 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    38881 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    38941 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    39001 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    39061 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    39121 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    39181 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    39241 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    39301 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    39361 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    39421 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    39481 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    39541 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    39601 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    39661 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    39721 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    39781 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    39841 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    39901 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    39961 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    40021 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    40081 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    40141 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    40201 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    40261 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    40321 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    40381 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    40441 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    40501 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    40561 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    40621 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    40681 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    40741 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    40801 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    40861 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    40921 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    40981 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    41041 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    41101 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    41161 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    41221 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    41281 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    41341 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    41401 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    41461 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    41521 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    41581 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    41641 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    41701 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    41761 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    41821 tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta gcaaacaatt
    41881 tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca ctcattgatt
    41941 acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata atcgtctttt
    42001 ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata tgctttacct
    42061 gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag gcagctaact
    42121 cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg acgtgcgaac
    42181 cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc attgtcagct
    42241 ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa gaaaagtatt
    42301 gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt ttcaggtatc
    42361 cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca atttccagtt
    42421 gagataagac agattcaaga tattggattt catctttggc ttcttggatt tgttcgccaa
    42481 tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt gcattttgat
    42541 ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca taataattag
    42601 gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta agtagttcgc
    42661 catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca cgttttttca
    42721 atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct ccgccttgtt
    42781 gttttgcccg atctttctct gctttttcgt gataatagct atctaataat tgactgagtg
    42841 ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca atcggtgtga
    42901 aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg atggctgaga
    42961 aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt ttggtcagct
    43021 cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt ggatctagtt
    43081 cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg ttcaagacct
    43141 cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa gtgttttgag
    43201 agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta actaagacga
    43261 tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga tcacccaact
    43321 cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt tcgatgtctt
    43381 ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc ggattatcag
    43441 gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc gcagataata
    43501 ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt tcataaggct
    43561 gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg accatcgcgt
    43621 gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata agtatattca
    43681 ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa agtctagaaa
    43741 aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa atttttaggt
    43801 ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg aagtagttaa
    43861 atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa atccaagtaa
    43921 gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag ttgcaatgtt
    43981 tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt cgtccatacc
    44041 aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca aacaaagccg
    44101 ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca tgcttgatgt
    44161 ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt atccgatcgt
    44221 ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag tgacatttat
    44281 tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag gaataactga
    44341 ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata tcaaacaatt
    44401 agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg ctaattgaga
    44461 cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca gaacaagtga
    44521 tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga aattcaaaaa
    44581 ttcgatttga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac ttatcaaaac
    44641 gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt gtattaatgg
    44701 tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt atctatgcta
    44761 gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg taaagacaat
    44821 gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta taataaataa
    44881 aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac agttgtagta
    44941 attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg cgaaaaagaa
    45001 agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca atacgtgagc
    45061 catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga agaaggatta
    45121 aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca gagcaagcta
    45181 gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg aatcgcaaca
    45241 cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt aggtgctgta
    45301 acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg taatgtaaca
    45361 ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga tttgttgcca
    45421 aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa ataccaagta
    45481 gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata tgcggtgcct
    45541 tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga ttttatctat
    45601 gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga agcaaacaaa
    45661 gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg actagcaaca
    45721 atcgggatca atcaatatca attagggctt caaagcggta agatggcggc agaacttgcg
    45781 agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg ggatacagtg
    45841 atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat gaaggaaaaa
    45901 gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga caaatgatcg
    45961 tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt ttcatgactt
    46021 atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta ggtgctgctg
    46081 tctgtgtgac agctattact catggggtag cgccgattgt agctacatta ttaggcgtag
    46141 gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga aagataccgg
    46201 ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg tttgtgatgc
    46261 gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc catcgctttg
    46321 ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc atcttgattg
    46381 ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg acaggagaca
    46441 acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt ttaggtttaa
    46501 ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac gacggatatg
    46561 cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc atcatcggtg
    46621 aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt gtcggcagta
    46681 tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg acttatctga
    46741 agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg aagaaagcac
    46801 tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat tgacaatcaa
    46861 aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat tggatgaaat
    46921 taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg gtgctggaaa
    46981 aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat cgatctcttt
    47041 taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc tttctcgagt
    47101 tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa atttgctact
    47161 tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa atcaaaaaga
    47221 agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact tggatactcc
    47281 agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg cgacgatcac
    47341 agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta aaacagctaa
    47401 agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt gtctcatgat
    47461 cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac tccaaaaagg
    47521 gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg gcgacctttt
    47581 gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc agtagaagga
    47641 cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa taaacaatta
    47701 aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt aaatagaata
    47761 ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca cctagacgtt
    47821 tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga acaaaggagg
    47881 aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc ggcttctcac
    47941 agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga tgcggtgtat
    48001 ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat tcgaactttt
    48061 gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc ttatctggat
    48121 gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca cgaaaagggt
    48181 cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca agaagctggg
    48241 atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc aaaggcagtc
    48301 actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg aaacaaccaa
    48361 acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc ttgtaagata
    48421 aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga aagctgtctt
    48481 ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc gctcttagat
    48541 ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc tagagaaaca
    48601 cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact tggcatgttg
    48661 atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc gatccatgtg
    48721 attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt ctcgcataaa
    48781 cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat tttttattaa
    48841 attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt tatcagtaag
    48901 aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca taagaaggcg
    48961 aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa acgaacgcgt
    49021 atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc atttgaacat
    49081 ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga acaacaaaaa
    49141 aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg aagaaagcga
    49201 tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc aggtatctca
    49261 ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc gacttcagaa
    49321 acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct tggaagtgat
    49381 acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt ggatggtcct
    49441 tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat cccaggggta
    49501 ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt gcgtcaaact
    49561 ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt caaatcgttt
    49621 gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga aaaagatctg
    49681 aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca tgttcttttg
    49741 cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag acgccaacag
    49801 caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat ccgtgcacct
    49861 tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg gttcttgacg
    49921 aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag tgtgccagta
    49981 gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga aattgataat
    50041 caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa aagatcaaga
    50101 agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta aaaaacgctt
    50161 tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat ctgaacttca
    50221 aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact gaagcatcag
    50281 gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca gaagaagcaa
    50341 aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg tttgcagata
    50401 cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta gtcatccaga
    50461 caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt ggtaagattg
    50521 agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact gatcgatttg
    50581 gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc tggaaacatc
    50641 aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt cgactatacg
    50701 gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag tccgcaaaaa
    50761 attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac cttcacaagc
    50821 aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt atgaaatcag
    50881 cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt tggtagatat
    50941 ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa atttgcctat
    51001 tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat tttcgatcgt
    51061 aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg atcgagaagg
    51121 tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg aaaagaacta
    51181 tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt ttaagaatct
    51241 ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc agaacgctga
    51301 ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac gaaaagggat
    51361 tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga tttatcagct
    51421 gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag gtttaattac
    51481 gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta aaacccaaga
    51541 agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa acggacggat
    51601 tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt cgctatctag
    51661 tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac ttgatgctgc
    51721 tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct tttacattac
    51781 agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat ttttaggaat
    51841 agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg cgaaaaaagt
    51901 tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac gctttgcgaa
    51961 gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg tggctatgtt
    52021 tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc aattaaagct
    52081 aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac gcatcaagtc
    52141 aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag cagaagtaga
    52201 actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag gaacgttgat
    52261 cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag aaattctcaa
    52321 agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa atcttaagca
    52381 gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag gaatcactct
    52441 ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg cgcgctggga
    52501 agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt tagagaatat
    52561 caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca aggtagactt
    52621 ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa acgatcaatt
    52681 aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa tcgctacatg
    52741 cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga agctgactaa
    52801 aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca aaggttttac
    52861 tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga caatcggttt
    52921 ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca aagaaacatc
    52981 aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc ggaatattta
    53041 ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga atcaagcaaa
    53101 aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt gaaaaaagtg
    53161 cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta ccaagcattt
    53221 ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca aacagcatat
    53281 tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac ttattttttt
    53341 cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt ggtggtggta
    53401 gcggctatga tcattattta ttttctacca gttattacac aagtatttca tcaatatttt
    53461 ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc tttgtcattt
    53521 tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat
    53581 aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa
    53641 aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa
    53701 gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct
    53761 gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg
    53821 aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag
    53881 aaattatcaa aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg
    53941 taaaagatgc aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc
    54001 aagaactcta tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa
    54061 aacatccttt tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc
    54121 aattgaataa ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga
    54181 ttaccactag ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac
    54241 aaaccgcaat aaaatgaacg aattaaaaac tttatttctc aattagatca gcatgtcatt
    54301 aaggaatatg ctgatttttt tatgtttaat catacaaaat agttttttca taaaaatatg
    54361 gaaaatatgg attaattttg aaataattga tttaaaagga atacaaaaga gtgaatagta
    54421 ggaaatagtg gtttagaact agtgggctga ttttttatat attttagttt acataatata
    54481 tattatacaa agtagattta aaaaatgata aacctctctc gaacggtttt ttatcaaaaa
    54541 tcgtcatttt tccatcaaaa tctgcttatg caaaattata aaaaaatttt gcagtttttc
    54601 ttactttttt tgtcgtttaa ttgaatgata aaaaccagtt tctttattca gttaatcaaa
    54661 tatgaacgaa ttagatatga atcattgtat ttgccgttgc ggaaggattt tttaattaac
    54721 cacagataaa cattgataac gattcgctta ttgtaatcaa cagattaaat ttcatatgtt
    54781 catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta tttgcttacc
    54841 tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca gttttaggat
    54901 ttttagttca tattcattgt ttcttataat acagtgttca atacttctct taaagcactt
    54961 gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac aacttcacga
    55021 acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc gtcgtattca
    55081 ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat agcttttacg
    55141 atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc aatgatctca
    55201 taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc aatacgatgg
    55261 tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga ccatgcagca
    55321 acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg gtcaactttt
    55381 aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc agtaccgata
    55441 acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat atctactggg
    55501 tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt tacgatttgg
    55561 cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg tttttggttg
    55621 ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc acctgaccat
    55681 acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc ttccccttct
    55741 gctttttctt gattcacatc gatcaatact aattcattag agattccttg gttgatcatt
    55801 gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt acgacttgtt
    55861 tttttcatga ataaacagct ccttttcata actatttgtt actttcatga aaagtatagt
    55921 cttgtttatt cattttatca acatgttaat tataatagtt gttattataa tttttgatgg
    55981 aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct atatcccctg
    56041 ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct gcaacgatat
    56101 aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg aaagattttc
    56161 ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct tgaataattt
    56221 gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca acgaaaaact
    56281 cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca gttcttgaaa
    56341 ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt agccaaaatg
    56401 agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat ctaatactct
    56461 aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt ctaataaatg
    56521 agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct tatgactgta
    56581 tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc
    56641 gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa
    56701 agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag ataaggtttc
    56761 ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga ttctttgagt
    56821 agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt ctccattaat
    56881 aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat gtgtatgctc
    56941 gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt gaaatagatc
    57001 ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat cattgatatt
    57061 cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta
    57121 attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg cttattagtt
    57181 cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact ttctatggtt
    57241 gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata ctatattttt
    57301 gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga tgaaattttc
    57361 tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg caattcccag
    57421 tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa cgactgctcc
    57481 ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga aaatcggaca
    57541 aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc tccccctctt
    57601 ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg gctttggatt
    57661 gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg aacgagcctc
    57721 tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga catttgttgc
    57781 aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca tcagcttacc
    57841 tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac ttcaatcgca
    57901 tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta gttatggtat
    57961 ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag ccacagtcct
    58021 gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt
    58081 ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac atcgctatat
    58141 gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca ctgtgataaa
    58201 cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt tattcattcc
    58261 ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt tcaatacttc
    58321 aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt tgctctttct
    58381 ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca gtatcttgct
    58441 gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa agacagtgat
    58501 aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg
    58561 taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag actttgagat
    58621 ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact ggccagcgta
    58681 ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact ttcttgatca
    58741 accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta atggacaaaa
    58801 acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt
    58861 aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa aatcagatat
    58921 tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc cgctttatgt
    58981 cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac
    59041 tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga atggctgtgt
    59101 agaactaata tctctggatg accatatcga atctatcact ctatgttatg gagagtccga
    59161 tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga caagaaaaat
    59221 gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta ctggaatggt
    59281 tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt tttcaagcga
    59341 ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt tatgtagtga
    59401 tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag atgcgtatca
    59461 atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac gaacgatatt
    59521 agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg actattcgat
    59581 cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg aatttttacg
    59641 catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa cgaatgaatt
    59701 agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg aaatggataa
    59761 agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt ccatgatcac
    59821 ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata ctcatctcta
    59881 tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg atgaagaact
    59941 tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat
    60001 tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct taaagaatgt
    60061 cttattgaat gacactatca cacagattac tactccatat ttcaaattct atgaacaaga
    60121 tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg attattgggg
    60181 agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta ctcaaacagg
    60241 aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc atgcatgggg
    60301 agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa caaagccagg
    60361 ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga attgtacgct
    60421 acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg tgattacttc
    60481 tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca
    60541 gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac
    60601 atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat tttgaagttt
    60661 gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag aaagctagat
    60721 aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg accgtattct
    60781 cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa tgtagaagaa
    60841 gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact aatcaataag
    60901 ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt gccgaagaca
    60961 ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac tgtagtttgc
    61021 aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa aaagccaatt
    61081 aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg tttagaatcg
    61141 ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa caccattcga
    61201 accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat cagtgcaaag
    61261 attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc agctaacaaa
    61321 gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga atctcgcaaa
    61381 aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac
    61441 tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa cgcaacttga
    61501 ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat agttaatagt
    61561 gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa ccccgcgctt
    61621 actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt aagaaaaatg
    61681 aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac tagcatgatc
    61741 aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc attgctagga
    61801 actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt tagtgaatta
    61861 ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt gatagttcct
    61921 tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc aatcaaagaa
    61981 acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt atccattgat
    62041 agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt tttcttttac
    62101 ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag
    62161 cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc
    62221 aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg
    62281 tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat
    62341 tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt
    62401 gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc
    62461 gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg
    62521 gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta
    62581 cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac
    62641 catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg
    62701 ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata
    62761 attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt
    62821 ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt
    62881 cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa
    62941 atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag
    63001 tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag
    63061 tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag
    63121 tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag
    63181 agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt
    63241 ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga
    63301 tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg
    63361 aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa
    63421 tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag
    63481 caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca
    63541 aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga
    63601 aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac
    63661 taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg
    63721 tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg
    63781 gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt
    63841 aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact
    63901 gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca
    63961 gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc
    64021 ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat
    64081 gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct
    64141 cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga
    64201 ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca aaaggagtta
    64261 attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga
    64321 tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct
    64381 acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt
    64441 ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg
    64501 tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta
    64561 ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct
    64621 ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat
    64681 aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg
    64741 tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc
    64801 attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct
    64861 agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg
    64921 attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc
    64981 caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac
    65041 taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt
    65101 ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa
    65161 atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg
    65221 atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc
    65281 tttgatgcgg aaataactgg attttatcga aattttcata tttccataga aaaaatccag
    65341 atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc
    65401 atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa
    65461 atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc
    65521 ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac
    65581 tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc
    65641 ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac
    65701 tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg
    65761 tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt
    65821 atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat
    65881 attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc
    65941 attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta
    66001 tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa
    66061 acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa
    66121 gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta
    66181 ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt
    66241 acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta
    66301 cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta
    66361 aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa
    66421 cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa
    66481 tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt
    66541 tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg
    66601 attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga
    66661 tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt
    66721 gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt
    66781 tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca
    66841 aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga
    66901 ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt
    66961 ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaaag
    67021 gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat
    67081 agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa
    67141 acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag
    67201 ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat
    67261 cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg
    67321 tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg
    67381 atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc
    67441 gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc
    67501 gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc
    67561 tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc
    67621 tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt
    67681 attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat
    67741 cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg
    67801 atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc
    67861 ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat
    67921 gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc
    67981 tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt
    68041 catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa
    68101 tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag
    68161 caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc
    68221 ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa
    68281 tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta
    68341 atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac
    68401 ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg
    68461 atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat
    68521 cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat
    68581 ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat
    68641 ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt
    68701 cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat
    68761 aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg
    68821 ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat
    68881 caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg
    68941 ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt
    69001 caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat
    69061 catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga
    69121 ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt
    69181 ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt
    69241 cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag
    69301 ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc
    69361 cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga
    69421 tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag agatggatag
    69481 tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata
    69541 cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga
    69601 tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat
    69661 cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt
    69721 ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat
    69781 aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc
    69841 cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt
    69901 gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta
    69961 aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc attttttcac
    70021 tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac
    70081 gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt
    70141 tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat
    70201 ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa
    70261 tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag
    70321 gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta
    70381 atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt
    70441 tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac
    70501 accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact
    70561 gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg
    70621 gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag
    70681 cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc
    70741 actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa
    70801 ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc
    70861 aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag
    70921 tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt
    70981 gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc
    71041 tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc
    71101 ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc
    71161 taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa
    71221 tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg
    71281 atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat
    71341 ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat
    71401 actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga
    71461 taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt
    71521 cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg
    71581 tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca
    71641 tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa
    71701 gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct
    71761 tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct
    71821 tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga
    71881 ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg
    71941 atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca
    72001 ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga
    72061 cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc
    72121 tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac
    72181 ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca
    72241 tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt
    72301 tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta
    72361 taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga
    72421 tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc
    72481 ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta
    72541 tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg
    72601 attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct
    72661 tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt
    72721 gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt
    72781 gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg
    72841 tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt
    72901 ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc
    72961 atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa
    73021 tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct
    73081 tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt
    73141 gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca
    73201 gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca
    73261 gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac
    73321 tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca
    73381 tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta
    73441 ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga
    73501 gaagtatttc ccggccgatc ctgcgtatta gaaaactgag ttccgtaata attgctgctt
    73561 gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa
    73621 acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct
    73681 ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc
    73741 actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag
    73801 tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca
    73861 aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac
    73921 cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca
    73981 ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca
    74041 tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga
    74101 tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga
    74161 tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca
    74221 tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat
    74281 aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct
    74341 atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata
    74401 atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga
    74461 tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt
    74521 ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag
    74581 taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt
    74641 agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt
    74701 tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat
    74761 aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt
    74821 cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag
    74881 gcgttacata attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga
    74941 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
    75001 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaacga
    75061 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
    75121 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtataatcga
    75181 agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta
    75241 tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag
    75301 ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta
    75361 ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa
    75421 tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag
    75481 ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg
    75541 aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa
    75601 taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat
    75661 gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg
    75721 agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct
    75781 cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat
    75841 tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat
    75901 gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca
    75961 attattaacg caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac
    76021 gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg
    76081 atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga tttattattt
    76141 atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga
    76201 taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata
    76261 tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga
    76321 tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt
    76381 gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga
    76441 actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga
    76501 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
    76561 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
    76621 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
    76681 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
    76741 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
    76801 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
    76861 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
    76921 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
    76981 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
    77041 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
    77101 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
    77161 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
    77221 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
    77281 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
    77341 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
    77401 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
    77461 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
    77521 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
    77581 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
    77641 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
    77701 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
    77761 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
    77821 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
    77881 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
    77941 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
    78001 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
    78061 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
    78121 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
    78181 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
    78241 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
    78301 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
    78361 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
    78421 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
    78481 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
    78541 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
    78601 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
    78661 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
    78721 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
    78781 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
    78841 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
    78901 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
    78961 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
    79021 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
    79081 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
    79141 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
    79201 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
    79261 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
    79321 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
    79381 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
    79441 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
    79501 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
    79561 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
    79621 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
    79681 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
    79741 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
    79801 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
    79861 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
    79921 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
    79981 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
    80041 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
    80101 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
    80161 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
    80221 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
    80281 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
    80341 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
    80401 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
    80461 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
    80521 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
    80581 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
    80641 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
    80701 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
    80761 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
    80821 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
    80881 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
    80941 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
    81001 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
    81061 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
    81121 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
    81181 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
    81241 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
    81301 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
    81361 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
    81421 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
    81481 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
    81541 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
    81601 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
    81661 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
    81721 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
    81781 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
    81841 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
    81901 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
    81961 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
    82021 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
    82081 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
    82141 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
    82201 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
    82261 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
    82321 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
    82381 tataaattta ttctatagga gacgattgtt ttctacaaat at
//
LOCUS       DACEKQ010000002        72177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEKQ010000002 DACEKQ010000000
VERSION     DACEKQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.002"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(507..1061)
                     /locus_tag="IW671_000074"
     CDS             complement(507..1061)
                     /locus_tag="IW671_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0897095.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1187..3116)
                     /locus_tag="IW671_000075"
                     /pseudo
     CDS             complement(1187..3116)
                     /locus_tag="IW671_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3422..4138)
                     /locus_tag="IW671_000076"
     CDS             complement(3422..4138)
                     /locus_tag="IW671_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0897096.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="IW671_000077"
     CDS             complement(4139..5962)
                     /gene="glpO"
                     /locus_tag="IW671_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0897097.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="IW671_000078"
     CDS             complement(5976..7472)
                     /gene="glpK"
                     /locus_tag="IW671_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0897098.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7608..9068)
                     /locus_tag="IW671_000079"
     CDS             complement(7608..9068)
                     /locus_tag="IW671_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0897099.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9058..10353)
                     /locus_tag="IW671_000080"
     CDS             complement(9058..10353)
                     /locus_tag="IW671_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0897100.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10685..11362)
                     /locus_tag="IW671_000081"
     CDS             complement(10685..11362)
                     /locus_tag="IW671_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0897101.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11392..12483)
                     /locus_tag="IW671_000082"
     CDS             complement(11392..12483)
                     /locus_tag="IW671_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0897102.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12584..13018)
                     /locus_tag="IW671_000083"
     CDS             complement(12584..13018)
                     /locus_tag="IW671_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0897103.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="IW671_000084"
     CDS             complement(13602..15059)
                     /gene="ascB"
                     /locus_tag="IW671_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0897104.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15056..16951)
                     /locus_tag="IW671_000085"
     CDS             complement(15056..16951)
                     /locus_tag="IW671_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0897105.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17009..18784)
                     /locus_tag="IW671_000086"
     CDS             complement(17009..18784)
                     /locus_tag="IW671_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0897106.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18834..20330)
                     /locus_tag="IW671_000087"
     CDS             complement(18834..20330)
                     /locus_tag="IW671_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0897107.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20524..22146)
                     /locus_tag="IW671_000088"
     CDS             complement(20524..22146)
                     /locus_tag="IW671_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0897108.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22474..23787)
                     /locus_tag="IW671_000089"
     CDS             complement(22474..23787)
                     /locus_tag="IW671_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0897109.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24032..24256)
                     /locus_tag="IW671_000090"
     CDS             complement(24032..24256)
                     /locus_tag="IW671_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897110.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24276..24644)
                     /locus_tag="IW671_000091"
     CDS             complement(24276..24644)
                     /locus_tag="IW671_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0897111.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24841..25551)
                     /locus_tag="IW671_000092"
     CDS             complement(24841..25551)
                     /locus_tag="IW671_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897112.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25569..26066)
                     /locus_tag="IW671_000093"
     CDS             complement(25569..26066)
                     /locus_tag="IW671_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897113.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26103..26555)
                     /locus_tag="IW671_000094"
     CDS             complement(26103..26555)
                     /locus_tag="IW671_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0897114.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26690..27295)
                     /locus_tag="IW671_000095"
     CDS             complement(26690..27295)
                     /locus_tag="IW671_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0897115.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27745..28365)
                     /locus_tag="IW671_000096"
     CDS             complement(27745..28365)
                     /locus_tag="IW671_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0897116.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28367..29236)
                     /locus_tag="IW671_000097"
     CDS             complement(28367..29236)
                     /locus_tag="IW671_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0897117.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29259..29666)
                     /locus_tag="IW671_000098"
     CDS             complement(29259..29666)
                     /locus_tag="IW671_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0897118.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29685..30974)
                     /locus_tag="IW671_000099"
     CDS             complement(29685..30974)
                     /locus_tag="IW671_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0897119.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31001..31303)
                     /locus_tag="IW671_000100"
     CDS             complement(31001..31303)
                     /locus_tag="IW671_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897120.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31328..33421)
                     /locus_tag="IW671_000101"
     CDS             complement(31328..33421)
                     /locus_tag="IW671_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0897121.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33910..34674)
                     /locus_tag="IW671_000102"
     CDS             complement(33910..34674)
                     /locus_tag="IW671_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0897122.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34785..35654)
                     /gene="mdcB"
                     /locus_tag="IW671_000103"
     CDS             complement(34785..35654)
                     /gene="mdcB"
                     /locus_tag="IW671_000103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0897123.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35679..36755)
                     /locus_tag="IW671_000104"
     CDS             complement(35679..36755)
                     /locus_tag="IW671_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0897124.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36779..37372)
                     /locus_tag="IW671_000105"
     CDS             complement(36779..37372)
                     /locus_tag="IW671_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0897125.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37376..39052)
                     /gene="mdcD"
                     /locus_tag="IW671_000106"
     CDS             complement(37376..39052)
                     /gene="mdcD"
                     /locus_tag="IW671_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0897126.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39042..39341)
                     /locus_tag="IW671_000107"
     CDS             complement(39042..39341)
                     /locus_tag="IW671_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0897127.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39443..41089)
                     /gene="mdcA"
                     /locus_tag="IW671_000108"
     CDS             complement(39443..41089)
                     /gene="mdcA"
                     /locus_tag="IW671_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0897128.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41096..42007)
                     /locus_tag="IW671_000109"
     CDS             complement(41096..42007)
                     /locus_tag="IW671_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0897129.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42127..42984
                     /locus_tag="IW671_000110"
     CDS             42127..42984
                     /locus_tag="IW671_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0897130.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43171..43533)
                     /locus_tag="IW671_000111"
     CDS             complement(43171..43533)
                     /locus_tag="IW671_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897131.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43879..45318
                     /locus_tag="IW671_000112"
     CDS             43879..45318
                     /locus_tag="IW671_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0897132.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45325..47877)
                     /locus_tag="IW671_000113"
     CDS             complement(45325..47877)
                     /locus_tag="IW671_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0897133.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47991..49322)
                     /locus_tag="IW671_000114"
     CDS             complement(47991..49322)
                     /locus_tag="IW671_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897134.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49288..50766)
                     /locus_tag="IW671_000115"
     CDS             complement(49288..50766)
                     /locus_tag="IW671_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897135.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51071..52906)
                     /gene="tdc"
                     /locus_tag="IW671_000116"
     CDS             complement(51071..52906)
                     /gene="tdc"
                     /locus_tag="IW671_000116"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0897136.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53376..54803
                     /locus_tag="IW671_000117"
     CDS             53376..54803
                     /locus_tag="IW671_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0897137.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54895..55260)
                     /locus_tag="IW671_000118"
     CDS             complement(54895..55260)
                     /locus_tag="IW671_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0897138.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(55257..55622)
                     /locus_tag="IW671_000119"
     CDS             complement(55257..55622)
                     /locus_tag="IW671_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0897139.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55814..56062)
                     /locus_tag="IW671_000120"
     CDS             complement(55814..56062)
                     /locus_tag="IW671_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897140.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            56061..56189
                     /locus_tag="IW671_000121"
     CDS             56061..56189
                     /locus_tag="IW671_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0897141.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56246..57940
                     /locus_tag="IW671_000122"
     CDS             56246..57940
                     /locus_tag="IW671_000122"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0897142.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            57944..58063
                     /locus_tag="IW671_000123"
                     /pseudo
     CDS             57944..58063
                     /locus_tag="IW671_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            58145..58336
                     /locus_tag="IW671_000124"
     CDS             58145..58336
                     /locus_tag="IW671_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897143.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            58714..59847
                     /locus_tag="IW671_000125"
     CDS             58714..59847
                     /locus_tag="IW671_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0897144.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59878..60198
                     /locus_tag="IW671_000126"
     CDS             59878..60198
                     /locus_tag="IW671_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897145.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(60227..60691)
                     /locus_tag="IW671_000127"
     CDS             complement(60227..60691)
                     /locus_tag="IW671_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897146.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60823..62077)
                     /locus_tag="IW671_000128"
                     /pseudo
     CDS             complement(60823..62077)
                     /locus_tag="IW671_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(62106..62423)
                     /locus_tag="IW671_000129"
     CDS             complement(62106..62423)
                     /locus_tag="IW671_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0897147.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(62449..62769)
                     /locus_tag="IW671_000130"
     CDS             complement(62449..62769)
                     /locus_tag="IW671_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897148.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(63233..64648)
                     /locus_tag="IW671_000131"
     CDS             complement(63233..64648)
                     /locus_tag="IW671_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0897149.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64665..65981)
                     /locus_tag="IW671_000132"
     CDS             complement(64665..65981)
                     /locus_tag="IW671_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0897150.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(66131..66829)
                     /locus_tag="IW671_000133"
     CDS             complement(66131..66829)
                     /locus_tag="IW671_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0897151.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(67118..68107)
                     /locus_tag="IW671_000134"
     CDS             complement(67118..68107)
                     /locus_tag="IW671_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0897152.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(68187..69020)
                     /gene="msrA"
                     /locus_tag="IW671_000135"
     CDS             complement(68187..69020)
                     /gene="msrA"
                     /locus_tag="IW671_000135"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0897153.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(69195..70823)
                     /locus_tag="IW671_000136"
     CDS             complement(69195..70823)
                     /locus_tag="IW671_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897154.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(70810..72144)
                     /locus_tag="IW671_000137"
     CDS             complement(70810..72144)
                     /locus_tag="IW671_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897155.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        21295 a        15184 c        12086 g        23612 t
ORIGIN      
        1 cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
       61 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa
      121 aaagtcgctt agtttcgagt attaagaaaa attttgtaaa aatggatttc tacatttttg
      181 cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt
      241 aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt
      301 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg
      361 tgagcctacc atttaactcc gaatcacaag aaataatctc aaaaacaact tctcctgttt
      421 ttggttcggt tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg
      481 aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca
      541 gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc
      601 cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca
      661 gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt
      721 aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc
      781 cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac
      841 ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct
      901 agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc
      961 agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga
     1021 atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc
     1081 agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt
     1141 ttatttttct ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt
     1201 catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag
     1261 ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca
     1321 gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac
     1381 gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta
     1441 tatcttcata aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg
     1501 caaagagctg acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa
     1561 attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca
     1621 atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt
     1681 ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc
     1741 acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg
     1801 atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca
     1861 tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt
     1921 ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa
     1981 ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct
     2041 tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac
     2101 acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg
     2161 atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata
     2221 atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt
     2281 gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct
     2341 tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct
     2401 tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa
     2461 accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg
     2521 gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt
     2581 tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt
     2641 agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta
     2701 ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta
     2761 taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga
     2821 ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat caaccctttt
     2881 ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg
     2941 ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg
     3001 tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct
     3061 tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc
     3121 tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa
     3181 ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt
     3241 ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac
     3301 ttactgatta gataccagct acactcacaa aacatacccc attcttctag gtcttattta
     3361 ttttccactt aactatagca agacacattt tggttgtcaa actttagtat gttttataga
     3421 tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga
     3481 ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat
     3541 gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta
     3601 atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa
     3661 attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg
     3721 tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg
     3781 cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc
     3841 ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg
     3901 cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc
     3961 cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc
     4021 cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca
     4081 aaactaggat cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt
     4141 atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag
     4201 tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag
     4261 gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga
     4321 gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact
     4381 cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg
     4441 cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta
     4501 ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac
     4561 ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta
     4621 ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc
     4681 caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg
     4741 tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag
     4801 cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct
     4861 tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca
     4921 gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat
     4981 agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg
     5041 taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa
     5101 tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag
     5161 gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga
     5221 ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca
     5281 ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa
     5341 cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc
     5401 catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat
     5461 ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata
     5521 cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt
     5581 cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct
     5641 catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga
     5701 ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa
     5761 taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt
     5821 cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga
     5881 ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt
     5941 gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt
     6001 actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca
     6061 aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca
     6121 gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg
     6181 atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg
     6241 ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata
     6301 tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta
     6361 ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc
     6421 ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct
     6481 tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa
     6541 ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca
     6601 attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt catgacaata
     6661 aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat
     6721 aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga
     6781 taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga
     6841 ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac
     6901 atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat
     6961 actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg
     7021 ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact
     7081 aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt
     7141 ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt
     7201 tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt
     7261 ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca
     7321 tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa
     7381 ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg
     7441 tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc
     7501 tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt
     7561 ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct
     7621 tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct
     7681 gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat
     7741 tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc
     7801 agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact
     7861 actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg
     7921 taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt
     7981 aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc
     8041 cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg
     8101 gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc
     8161 cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt
     8221 gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg
     8281 taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg
     8341 atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc
     8401 aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa
     8461 gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga
     8521 atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata
     8581 tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg
     8641 tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa
     8701 acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt
     8761 tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt
     8821 ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact
     8881 tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac
     8941 gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc
     9001 agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca
     9061 atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg
     9121 ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat
     9181 tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat
     9241 ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag
     9301 cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt
     9361 caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc
     9421 ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc
     9481 gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc
     9541 atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt
     9601 tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt
     9661 agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc
     9721 attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat
     9781 ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg
     9841 gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc
     9901 tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc
     9961 cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg
    10021 aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc
    10081 tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat
    10141 tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac
    10201 ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc
    10261 aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc
    10321 tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt
    10381 ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat
    10441 acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca
    10501 ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg
    10561 tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa
    10621 aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt
    10681 atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat
    10741 tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact
    10801 gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg
    10861 taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag
    10921 attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga
    10981 ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa
    11041 aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca
    11101 catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat
    11161 aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc
    11221 ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag
    11281 tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg
    11341 ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc gttattgata
    11401 acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc
    11461 aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg
    11521 atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac
    11581 atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt
    11641 atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata
    11701 catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca
    11761 gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc
    11821 tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc
    11881 ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga
    11941 atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc
    12001 aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat
    12061 ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact
    12121 tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa
    12181 aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg
    12241 aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg
    12301 gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt
    12361 gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag
    12421 catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga
    12481 cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa
    12541 ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact
    12601 tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg
    12661 ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc
    12721 cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt
    12781 tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg
    12841 caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag
    12901 ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta
    12961 gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt
    13021 catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg
    13081 aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt
    13141 tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt
    13201 cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    13261 atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa
    13321 agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg
    13381 ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc
    13441 ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg
    13501 actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg
    13561 gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt
    13621 gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca
    13681 ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta
    13741 gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct
    13801 ttgatagctt ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct
    13861 tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac
    13921 agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca
    13981 atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc agttattaag
    14041 tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag
    14101 ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt
    14161 tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt
    14221 gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca
    14281 tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa
    14341 cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt
    14401 gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta
    14461 acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta
    14521 tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga
    14581 ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa
    14641 acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat
    14701 cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac
    14761 atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga
    14821 tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct
    14881 tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg
    14941 ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc ccatgcacct
    15001 tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata
    15061 tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga
    15121 tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt
    15181 tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat
    15241 cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga
    15301 tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg
    15361 ttgaaacttc cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc
    15421 cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt
    15481 cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct
    15541 tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa
    15601 ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa
    15661 ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac
    15721 aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga
    15781 ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg
    15841 ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg
    15901 gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc
    15961 aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa
    16021 cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg
    16081 caattggacc tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg
    16141 tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa
    16201 gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg
    16261 gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa
    16321 cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag
    16381 tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga
    16441 tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt
    16501 tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt
    16561 ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat
    16621 cttctagttt catttgtttg gataatacgt catacacttc atttactgca tttcctacta
    16681 cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct
    16741 tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt
    16801 aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg
    16861 aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat
    16921 aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg
    16981 aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga
    17041 aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc
    17101 tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc
    17161 ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac
    17221 taggagagat tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt
    17281 ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt
    17341 cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa
    17401 tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg
    17461 ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc
    17521 acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt
    17581 tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca
    17641 aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac
    17701 gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca
    17761 tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt
    17821 ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa
    17881 atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc aaataattta
    17941 actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt
    18001 ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg
    18061 ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta
    18121 tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt
    18181 ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggcaac cttgcgtgtt
    18241 ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa
    18301 tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc
    18361 aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg
    18421 ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag
    18481 ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt
    18541 tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga
    18601 ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg
    18661 tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta
    18721 caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa
    18781 acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg
    18841 aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt ataatattca
    18901 aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa
    18961 ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc
    19021 caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga
    19081 tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct
    19141 tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat
    19201 tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat
    19261 ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct
    19321 gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta
    19381 gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat
    19441 gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa
    19501 ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc
    19561 gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat
    19621 aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt
    19681 tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca
    19741 gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact
    19801 ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt
    19861 cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga
    19921 gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg
    19981 agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg
    20041 cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga
    20101 tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc
    20161 caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc
    20221 gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca
    20281 gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct
    20341 cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct
    20401 aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat
    20461 atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac
    20521 gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg
    20581 taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa
    20641 cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg
    20701 agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat
    20761 gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg
    20821 atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg
    20881 aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt
    20941 gtctcttcct ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat
    21001 cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg
    21061 gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg
    21121 gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg
    21181 agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc
    21241 taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa
    21301 atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat
    21361 ggataattca tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca
    21421 tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag
    21481 atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat
    21541 atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg
    21601 ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata
    21661 caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat
    21721 tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg
    21781 ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg
    21841 attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc
    21901 ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata
    21961 cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa
    22021 atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt
    22081 tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg
    22141 ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag
    22201 tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca
    22261 gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt
    22321 atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc
    22381 ttggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
    22441 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
    22501 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
    22561 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
    22621 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
    22681 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
    22741 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
    22801 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
    22861 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
    22921 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
    22981 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
    23041 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
    23101 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
    23161 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
    23221 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
    23281 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
    23341 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
    23401 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
    23461 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
    23521 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
    23581 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
    23641 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
    23701 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
    23761 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
    23821 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
    23881 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
    23941 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
    24001 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
    24061 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
    24121 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
    24181 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
    24241 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
    24301 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
    24361 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
    24421 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
    24481 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
    24541 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
    24601 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
    24661 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
    24721 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
    24781 caaacttttt ttacaaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt
    24841 ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa
    24901 tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc
    24961 agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct
    25021 ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat
    25081 ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc
    25141 ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg
    25201 tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa
    25261 ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt
    25321 agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt
    25381 ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt
    25441 ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt
    25501 tgcttcagga aatacttccc agaaattggt atccactatt acttttttca tctgttcaca
    25561 cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc atctcccata
    25621 taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca
    25681 tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca
    25741 atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag
    25801 attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt
    25861 agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta
    25921 tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa
    25981 tgctccggtg aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt
    26041 agtaagtcta catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc
    26101 tttcaataga tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg
    26161 catactttat ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat
    26221 gcgactgcat tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt
    26281 ttagtcggat agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc
    26341 ccattggatt ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt
    26401 tctgctagtt tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac
    26461 tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg
    26521 gcaatcattc cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt
    26581 aagactagtt cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc
    26641 ttttttaata actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat
    26701 agatctggtt tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata
    26761 gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa
    26821 acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct
    26881 gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg
    26941 ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt
    27001 cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct
    27061 tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa
    27121 tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca
    27181 atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata
    27241 gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct
    27301 tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta
    27361 cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca
    27421 aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg
    27481 taataagccc caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa
    27541 ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct
    27601 cggtcacaag tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg
    27661 tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt
    27721 tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc
    27781 caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc
    27841 taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat
    27901 atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat
    27961 atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc
    28021 aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg
    28081 gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc
    28141 ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc
    28201 cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc
    28261 gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt
    28321 tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta
    28381 agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta
    28441 ctgcttcatt tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa
    28501 aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc
    28561 ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga
    28621 taactagggg gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg
    28681 tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg
    28741 tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga
    28801 catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt
    28861 gcgcatgggc atattctact actgcttttg tcttcaggat attttctttg aaaggcagat
    28921 gggacccatc atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa
    28981 agtctcgggc gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg
    29041 tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa
    29101 taatgatcgg cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt
    29161 tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt
    29221 tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt
    29281 acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga
    29341 tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta
    29401 tacccatctc cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga
    29461 tcgttcacat acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta
    29521 tgcttatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt
    29581 tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt
    29641 tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa
    29701 actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct
    29761 gtagcattga tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta
    29821 ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg
    29881 ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc
    29941 gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg
    30001 aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg
    30061 atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac
    30121 agtagacgta caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa
    30181 aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca
    30241 aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct
    30301 tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct
    30361 ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga
    30421 cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc
    30481 atagcaatgg cgaaacaagc tccgataaaa tcaccgacca agatcaatac gatccctcgc
    30541 agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca
    30601 gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg
    30661 gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg
    30721 atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg
    30781 aaataagtta aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg
    30841 agtgtcttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc
    30901 aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa
    30961 ttgataacgc ccatgttttt accctctttt ctttttgtat ttagataact cccttatctt
    31021 gcagaactgg ggtgattttt tcttcaattt cattttttga aacaatattt ttcaaataga
    31081 tcatcgccgt tttaggatca actgcgaact tttcgaactg cgaacgaaaa ttttctgctg
    31141 tgatgatcac atctggtttc atggaaactg cggaagaaat atcacaatgg tccagtttgg
    31201 cttctactcc taatttgttc aaaacatcct cagcagacat ctgtgctgca aaactacttc
    31261 ctaaacctgc accgcatacg aataaaatgt tcaatttgtt catctttgtt ctcccccgta
    31321 tcatttatta tttctcaact actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc
    31381 attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa ttcaattagt tctttcatga
    31441 tgttcagatg cgagtaggag tctactgctg ccaaacaaaa aataatcttt actggatcca
    31501 tttcaggatt accgaaatcg attggttgct caatcgtagc tacgctgact cctaatttat
    31561 tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg tttgccaatc acgatatacg
    31621 gtccgtaatc attcactgca cgaatcatcg cttcgacata gcgctcttcg acaatgtttt
    31681 ctttaaccaa tggtctagca gctagttcaa tcgattcctc ccaagtagtg gctttttctt
    31741 tgatcatgat attgctgtcc gtcaaaatat cttttaacat aggctggatc tcccttttgt
    31801 tgatttctaa attgtttgtt tcgaataatt tttctaactt gccatagatg attcgatcta
    31861 ctcggccacc actttctttc atgacatcta ttatttgagt gaataactcc gtcgattcat
    31921 gactgttagg ctctaaccgc tgtagttctt catttctttt caaaaagtct tctataacca
    31981 agatattcgt ctcagttaaa atcggatcga caactaaaag cggcttattt cgataattca
    32041 attgaaaggt gctgaaaacc agattgacat ccaatttatc tactaattcg atttcttttg
    32101 aacttaaaac tgcagtaatc tcgatctgag gaaacttttc tttcagattc tctgctaaca
    32161 gatttccagt agccatccca tggttacaga caacgactga tttataaaca taattcaagt
    32221 ctcgcttgat tacactggcg atcgtcgaaa agtggatgac aagaaaagct atctcgtcct
    32281 ccagtatctt gccaccaatt acttcttcga tcgtaggaac aaaatgagtg actgcgcggt
    32341 agattgggaa ataatttgtt cggatgtttt ctttcaatgg attcatgact tggatatgat
    32401 tcttgattct tacaatcagt gcagccatgt gcttatagag attttcgaat aaagcttctt
    32461 cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta
    32521 gtaattgagc ttgtacccac tcgattgaat tggagatatc tctagcattg aacgtctcaa
    32581 tagtgaaacg aagataattt ctttctactt gcgggacatt gtccaattga aattcctcga
    32641 tcatgtggtc aataaatgca gaaaagctac tgtcttttat ttctgtattt ttccaattaa
    32701 tggcagagat aaattcatgt ttcctgattc gctggatcca gatcaatgta aaaagtagga
    32761 tttgtttttt ataaatatca tcttcttgtt tcaacaactt attggaatag atctcctgta
    32821 tttttttgat ttccaccaaa ggaaaataat ggagaaagat tttttgagcc ggtgttaatt
    32881 gtgcatcttg attcataaag aagtcggtca ttcccagatt ctgattgata aaatcataga
    32941 tcatcgtacg gatagatcgt tctgaacctt tataaaccag tccttgcttt ccataactga
    33001 cgatctctat cccataactg accagtgttg ctcgtaaccg tctcatatct tcatctaatg
    33061 tacttttaga gatttgaaat tcttcttctt ttttgtttag atggatcggg attcggctgt
    33121 aagcaacggc taaaactaga tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca
    33181 catcttttat ttcagtttct aatcgagctt gcttttcctt ttccagcttc aacaaaaaac
    33241 ctttgttgcg aattgtacgg atctccggaa gctttttact gctcaaccag atattcgctt
    33301 caagaacttc ctgacggatc gtccgctgac tgacattgaa ctcacttgct agatgttttg
    33361 cttttactgg atattgggct gtcatgattc tgtgaagtat ttcaattgtt cttgttttca
    33421 tagaaatgat ctccttctga tttcaagtat atggttccgc ttacataata aaaagtagat
    33481 atttctgcca cttgaagtgg aaagaaagta agcaaagcaa acaaaaaata agccatatgc
    33541 tttttcatct cctcaacgaa aaagacatac gcctattatt acaaaaattt tgcccatcta
    33601 tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca actaaactaa
    33661 aatagtgctt cctatttttc gtttggttgt tcttattatc gcagatgcaa gcttttaaac
    33721 accatttagt ggagaggttg tgacagagat gttcagcctt gagcgataag aagcgatttt
    33781 gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt gccgataggc tatctctggc
    33841 acaccgttta ttcagaaaaa ggagctgcga caaggctttt gtcacaactc cgttcataaa
    33901 tatttcctgt caatccagca taatcggctg aataaaatcg gcttgccatc ttttttcttt
    33961 ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt cgttgtgtct tacttcctga
    34021 gatcaatcgc ccatcttttc gatcgactga ataatcacag catctgaaat caattcggat
    34081 ggcaaaacta ttttcgtgga tctgtcgata atttcctact tcctgaatag ttattttttt
    34141 agtatgattt cttacaccgt ctgctttgtt tttttgcaga actttctgat gttcggcaaa
    34201 cagcttgtct gtataacagc ttctggcttt ttcaggagtt tcttcttccc aagcggattg
    34261 gatcttcatg aatatctttt ggatttccag aaactgtgct ttcttttctt tagccgttcc
    34321 tgacatgcgg gacaatatct ccgctttcag ccttttcttt tgtcttttat tcttgacgat
    34381 agaaaaagca gaaacccctc ctactgacaa aacagctaat gcaagaaatc ccgtttgctc
    34441 ataactagaa ggacctgtac ctggaccata atacccataa gaatctctgc cgtaagaacg
    34501 actgccaaaa taactgcccc catatgaacg accaccaaat gaacctctgc ccgaacttcg
    34561 accgctagat gtacctccac catgaccacc cgcgacagcg aatccttggg tcggcagagc
    34621 aaacaaacat aggcccacta acaaaatgat tgctaaataa ttgatttttt tcattgtctt
    34681 cctcgttatc ttttattaca aaaaactatt tttatgatag ccttgctaaa aggaagagtc
    34741 aataaccaga tgcataaact gtttttttag caattttgtc ttatttagtc attgagtaac
    34801 atcggacaaa tgtatgtgtc ggcagtccat aattcctcta tcttcagcat gaacaagctt
    34861 gcagacaaga gatctgcgct tcctccagga gagatttggt tttcctgaca aaagtgcaac
    34921 agtgctgtaa atggacgatt tggtaaagca ctttgactag cagcatgagc aagtttttgc
    34981 atttccacta acgttttctg atcactgcga tgcaatacgc atgtgtcatc tagcgaactc
    35041 atcaaaacta acaacatatg catttgtttc tgtacatatg aatcattatc tagctcctta
    35101 gctgcttgaa ttgcaagcct gatatgcgga taccctaata tcgcttcttc atatgcacca
    35161 tttacgccat atttttgctt caccctatga ccatgagtgg tttttttatg acgatatttt
    35221 gtatcaggaa aagcagcaag cttgctgaca tcatcaagta ttttcgataa agaaaaaggc
    35281 tgttccatac tgattttttg tgcaatgaca ctgatcaata gccccattgc ccaaatcgct
    35341 cctttatggg tattgatgcc atttgtagca atcagcattg ccttttccgc ttttcgccca
    35401 atctcagcaa ttctttctct taactcctga tcaatagcat gatgccatgc tatttgagcc
    35461 atttcttcaa aaaaaggagt tagcgtctta gcagacttaa gaaataaagc atagtccata
    35521 tcggaatgtg cacccgcatc cagtgcatcc actaatccag gttttggact taatgttact
    35581 tctgcgatca atgcttcctc taccaacgca gcgagttgct gagtcactgc tttttttcgt
    35641 tcgtcattta tcatgacact cactccttct tcctactatt acttgctgaa accttttttg
    35701 atagctaccc aaatgatatg aagtaaccag cttaaagcaa atgcacaact tgccgtcatt
    35761 agataagcta caagaatcat gactcctgaa ggtaactgca gccaactatc tttaaacaat
    35821 aataacagta cttggaaaac aaatacattg gctaaatacg tacgataagc ataaacagac
    35881 aaccatttga acaatggcaa ttttggcgaa tcgtttacaa tcattttgga agcgcctatg
    35941 aagacagcaa agatgatgac taaactgtat aaactttgga tcgtattcaa gtaaggtgca
    36001 ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac ggacagaaaa gaacgcgata
    36061 actaaggcaa caggtaagaa aagataacgt actctgtcca aatattgata gactttttcg
    36121 tgataaacaa atgcggctac cccataaatc atgaaactag caacaaaccg atctaataag
    36181 taccagctgt catgataagg cccagtcaat acttgtgttt gatagaacag ataccatgcg
    36241 acatataaaa tcgttgaacc aatcaatact ggccatacat attttttatt agcaaatact
    36301 ttgtaaccga gccaaacaaa aaacggcatg aacaattgga tctgcagcat cataacggta
    36361 taccataaat gaggcgctcc atcacctgtg acatatttca gtaaaaagct tgcagtatcc
    36421 gtatacggag tttgttgttg gactgctgga aatagccaaa gataagccgt cgtccataga
    36481 agatacggaa cgactagttc aaaaaacttt tctttcaaaa attcccaata agacaaatgc
    36541 tgacttgttt ttaccatatt ataaacaatt gcaaaaataa acattggtgc tgtgtattta
    36601 acgatgacgt aaaaggtacc aagaaaagct gtatttgttg cgctgtggct gtttgccaaa
    36661 gcaaagctca atatcgtttg catcataaca gccgttacag ctgatacttt ctggaagtca
    36721 cttacgttga ttttgatttt tttgttttca aacatctctt tctctctctt tttctttatt
    36781 accaaagttc tttggttaat ttaggtcctt cgactgtttt caatagaatt tttttctctg
    36841 gctctctcaa atactctttt agtgaaaaac cgccttgccg agtcactact tgggtatcga
    36901 attgctcaaa aaaagcagga tgcgattgaa ttacctgaat tggcaattca atcggtgaat
    36961 ttgcgtacag cagcaaatca aaatcactgg tttcttttac tgccggaatc ccagtcgata
    37021 attcaaatcc taagctgccg ccgattcccc atttacatcc tgataatatg tttctagcag
    37081 catgaaattg ctgataaaca ggataatcca tgattttctc ttgtctatac gattcttgtt
    37141 cgatgatgtc ccacggatga atgacttgtt tgacggcatg ttttgagatt tctgttgacc
    37201 atctctgatt gcgggcaggt ccacgtaaac caatcggtag tatttctgcg tgatccgtcg
    37261 acgcacgacg aactgttccg aacatctttt cagttagaac gacccattca ggcaaggtga
    37321 acccttctat ttcatgttta tcaaaaagga caatatcatg tgcttgaatc attttttatg
    37381 catcccattc ttcgtccatt ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt
    37441 tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc atttgtattg cggacatctt
    37501 gaatggcttc attgattgct tcgatgactt tgctgacatt ttgttctgaa gcagcttggt
    37561 cggtgatatc ttctaggaaa tgatagatcg ctcctagttt tgtataatta ttgatatcgt
    37621 aagcgatagc tggaactttt tcagcagctg cttcgatttc agcaaccgaa cgttttgtca
    37681 cacgtgcagc actttcttta gacatcgctt ggacggtaat ttctttattg tttaaagcaa
    37741 tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa
    37801 tcaatccaat aactggatgt ccagcttgac ggagttttgc ataagcagca gcactattag
    37861 ccaaagccac atggataccg atcaattctt cgttatagcc gtatgcttgg cttggtacat
    37921 cgatcacaac gatgattggc cgtttcactt ttttgtcttt atcttcttcg taaacgtgat
    37981 ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac ttcaccgttt cgtacacggt
    38041 acatcgggtt tttaggatca ggtacgattg atgtcacgat ggcttcttct atgaatttac
    38101 ttgatttacc ataatacaca gtactaatag aagaagttgg atttttcatg ccagttagtg
    38161 ccgcaaacca atcgtaacca acacttgctg cagctggttc atttccttct gctacttctg
    38221 gtaagtcaat tgattttgct tgatggttag caattgcttc attgtaatcg tcaatggcca
    38281 ttggttttga aagatctaaa gaatcaagta atgataaata aaatgctgag ctttcactgc
    38341 gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc agctttgatt gcttctacac
    38401 tatcagtcac taattcatca atgatttttg attctacccg ttgacgagat cctaatgtat
    38461 tccaaatcaa gtctttgtca ctagaatcaa attctcgaac gcctgcttct tgttcaataa
    38521 cttctggtcc attcaaaccg actctcgctt tttttgttgc gattaaataa ctcattaatg
    38581 ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg
    38641 gtacatattg ttttagagca atgattacat ttccgatttc tgaaatagat aacagtccgt
    38701 agtttgcttc ttgcaaacga acaccacctg tatctagtac aatgactgga aagatctcat
    38761 ttcctttttt attttcttct aacacatgtt caagtgcagc aacgattttt gcaccggaaa
    38821 cttcaccgat accgccacct tggaattttc cttccatagc gattactact gcttgtttgc
    38881 cattgatcgt gccacggctg acaacgatcc cgtcatcact ttcagggacg attccttgtg
    38941 ctggtaaact tggcgcgatg atttgatcga atggatcaat caattcacgt ccattgtcaa
    39001 gtagagcgtt cactcgttct ctggcgttta attctacaaa actatttttc atctgaaagt
    39061 tcctccattg cttgcgtcaa tcgtaggtac acaactcctg gtgtagcacc gaagtcattg
    39121 atcacgatat ctgctgaaat tggataacga tcaaaaaatc tcgttaatac attgttccat
    39181 acttcgccga agccatcgct tcctgtacaa atttttacag tagattctgt tttttctgtt
    39241 ggaaacatca agatttccaa atctcctgaa ccgaccactc ctacatggat tttcttattg
    39301 atcggctgtt tcgttggata tttataagct aattgttcca tggatctctc ccctttttgt
    39361 aaaacagagc atacacgttc tgcttattcg tctttcttct tccatctggg taaatggcga
    39421 caaaaggttt ttctcgccaa ttttatgacc aagttctgaa tttcattggt ggttcataca
    39481 ggccatctga ccattcgatc aaatcatcga tggtttgtgc agctaataat gaacgtttcg
    39541 cttcactacg tttgatgcct agatcttctg gtaatttcac gatgccggct gcacgcaagc
    39601 tatctagtga ttctttcgtg ctagtcattc caagaggtgt aacaccgcca attgctgcaa
    39661 ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa ataagcaatt ccttcttctg
    39721 tcacgatatg tgtagtatct tcgctataaa tcatcactgg tgcattggct aagcctgctt
    39781 gtttttgaac agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat
    39841 atgtttcaac catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc
    39901 gaagtgattg ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag
    39961 gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat
    40021 ctaattgcaa ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata
    40081 atgttcggtt actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag
    40141 caatatattt ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg
    40201 attcgatggc tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc
    40261 cttttaaccc tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga
    40321 atccaatacc gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca
    40381 tcattgccat tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt
    40441 ctaattgata aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta
    40501 cacgtggtaa agaatcgaca acttcgttga cttgaacgat cacgatacca tctttgaaag
    40561 ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt
    40621 ctttatctgc tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc
    40681 gtccataaag ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca
    40741 tttgggcgac acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc
    40801 ctttttcaaa aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat
    40861 cattcacttt ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt
    40921 gattgtcgcc ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta
    40981 cgattttttc agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct
    41041 gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc
    41101 tccatttttg atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac
    41161 ggatcccttt atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg
    41221 gaaattcaat cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact
    41281 gcacagggaa agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg
    41341 ctcttccatc cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca
    41401 tatacggctt gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga
    41461 ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata
    41521 aaccttcgat actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa
    41581 ctggatggtc aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac
    41641 ctactactac gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt
    41701 gatagactag ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa
    41761 aagcaccaat cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat
    41821 ctaaataaaa tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt
    41881 tttcttcttt atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt
    41941 cagggatatc cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg
    42001 tactcatgag cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct
    42061 gctataataa aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg
    42121 ataattatga aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt
    42181 acccaagcag ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat
    42241 ttggaaaacg aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc
    42301 gtttatgcag aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga
    42361 atcaaattat tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag
    42421 gtagcctctg tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc
    42481 caattaacga tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc
    42541 acttcggaga tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc
    42601 gccattccta agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa
    42661 cgcaccccca tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca
    42721 cttgaagcga aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta
    42781 cgttctattg tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac
    42841 gataaaaatg atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt
    42901 tatttatctt ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg
    42961 cgggaatttt atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct
    43021 cttttttcga acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc
    43081 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg
    43141 tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat
    43201 caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc
    43261 tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc
    43321 aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat
    43381 tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa
    43441 tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt
    43501 gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc ttattgattc
    43561 gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa
    43621 tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt
    43681 gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg
    43741 gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa
    43801 acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa
    43861 tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt
    43921 cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa
    43981 ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc
    44041 tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac
    44101 cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc
    44161 gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac
    44221 catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca
    44281 agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt
    44341 gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc
    44401 gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact
    44461 gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc
    44521 cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac actctcctcc
    44581 ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact
    44641 gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga
    44701 caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta
    44761 gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac
    44821 ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt
    44881 caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa
    44941 gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc
    45001 caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat
    45061 gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa
    45121 gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt
    45181 tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct agatatccct
    45241 attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact
    45301 ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt
    45361 gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac
    45421 atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg
    45481 gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa
    45541 aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt
    45601 gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct
    45661 ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata
    45721 gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg
    45781 cttcatgatt tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt
    45841 taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag
    45901 tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt
    45961 ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt
    46021 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    46081 agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    46141 tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt
    46201 cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag
    46261 atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat
    46321 cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg
    46381 gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc
    46441 tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt
    46501 ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca
    46561 tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt
    46621 gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    46681 actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg
    46741 aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt
    46801 ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac
    46861 atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt
    46921 catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat
    46981 ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat
    47041 tgacaccacc aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa
    47101 tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt
    47161 taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg
    47221 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    47281 agattgtaca agcgttcctg aaaaacccat atttttctgg tcgccgatcg attcttcttc
    47341 tgtcaaaacg gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
    47401 cactaataaa tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg
    47461 taagacaatg acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt
    47521 tgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc
    47581 ttttctttct tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact
    47641 tccttcaata tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat
    47701 cagcagatca gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt
    47761 tatttcatta tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc
    47821 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
    47881 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
    47941 attatttcag gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata
    48001 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
    48061 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
    48121 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
    48181 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
    48241 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca
    48301 aaaaataaca ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
    48361 caaaaccaat ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg
    48421 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
    48481 gctccgcctt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
    48541 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga
    48601 atacgccaag gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg
    48661 ccaacggtta agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag
    48721 actaccattc cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg
    48781 attccgatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
    48841 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
    48901 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
    48961 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
    49021 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
    49081 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
    49141 atggcccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
    49201 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
    49261 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
    49321 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
    49381 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
    49441 aattagattg atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
    49501 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
    49561 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
    49621 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
    49681 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
    49741 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
    49801 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
    49861 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
    49921 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
    49981 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
    50041 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat
    50101 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
    50161 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
    50221 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
    50281 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
    50341 caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
    50401 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc
    50461 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
    50521 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
    50581 agcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
    50641 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
    50701 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
    50761 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
    50821 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
    50881 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
    50941 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
    51001 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
    51061 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt
    51121 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
    51181 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
    51241 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
    51301 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
    51361 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
    51421 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
    51481 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
    51541 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
    51601 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
    51661 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
    51721 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
    51781 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
    51841 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
    51901 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat
    51961 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
    52021 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag
    52081 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
    52141 ggatagcaac catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat
    52201 agtgttttgt ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg
    52261 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
    52321 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca
    52381 gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa
    52441 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
    52501 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
    52561 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
    52621 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
    52681 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
    52741 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
    52801 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
    52861 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
    52921 tattttgttg gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata
    52981 gcgccaaaaa tgagaatttt ttttttataa catcaaatcc acgtagattt ttacaaacaa
    53041 aaataaataa agtcggattt ttctgataat tcaacgcaaa aaagcgaaca atttttcaat
    53101 aaatcagtct tctaaaatag ataacctaaa aaaagcgaac aaacaaataa aatgactgtc
    53161 cttcattcac taaacatatt tttatttttt tatattgtta aaaattcgca atttattttt
    53221 taatagaatt atctgttttc caaattttat aacataaaaa acctttttta acggcattta
    53281 tatgcttttt cagaaattaa ctgatgattt caaaaagaaa acatgtcata atatttccca
    53341 tattgtgcaa aaaaatacaa ggcggaggct ctactatgat tgaagaatat attgagaaaa
    53401 atattttaag acagctgttt ttatgtgggc aattctatgt aaataaagaa gtcaatctag
    53461 aaaagctctc caatctcctg catgtttgca aaaccacttt actcaatgat atcaataaca
    53521 taaaaaaaga atttgaagaa cagatcgctt atacacatcg ggaaaaggac cgttacactt
    53581 tatatttttc agaacatata cctcgatgca agatcatgca gcaattgtct caaaattcgt
    53641 tgtttttaaa aacttgtctg ctttatttgg aagaagatga acctgattat cttcaattaa
    53701 ctgaatgtga attcatctct gtcagcaaag cttattcatt aaaaaaacaa gtattagctt
    53761 attttaatga ctgcgggatc gaaatcgacc gctactcccc tcgattcact gaaatggaaa
    53821 gacgtctgct gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct tgggagctac
    53881 ctgaatcgtt ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa aatagtggca
    53941 gattttatga taaagaaaat aaagaaatct tatcgattgg ttttgctatc agtttcctac
    54001 gccagcaagt gtgtgctgtg acaatagatt caaaatttat cgaagaaatc aaaaaacgtc
    54061 ctatttacaa ttatgtcgaa tcagcatggg aaaacactga tttccaaacc tattataaaa
    54121 aagaagaatt cgcctttatt ttgacactat tcaatctttg taattatggt ttccattctt
    54181 atcagttgat tgcggaagac tttcaacagc tacaccaagt gtttattgac aatactcccg
    54241 aaataaaaga actagttgct acctttgaat ctcacttcaa tcaagaatta ttcggaaacc
    54301 aaccgttcga acgtgcgctg attcatctga tgcgttcggc ttgggataac tatcaattat
    54361 tcatgccaga aaaattttat cttttaaatg aggaacagac aaatttatat aaagaagtcc
    54421 agacaatttt ttccagctgg tccagccagt taccttatga tctacggttg aatcctaatt
    54481 gcatgcgggc ttttgtgatc gaattgagcg gaattctccg tctgacaaaa gaacatctga
    54541 ctatctatat cgtcacaaat tcggatgttc attatctgat ttatcgggaa gccttggaag
    54601 ctgtaactac tttcgatttt caagttgcac caacaattta ttcaagtata tctgatataa
    54661 agaagtatgc gcagcagtcc tcaaaccggg tattatgtga acgtacatta tatacaccag
    54721 atgcggtcca atatgaaaat attattccta tttcgatcaa tacgatcgat cgagctatca
    54781 tttcagctgt gcaaaacaaa taaggggctg tgacaaaaat catgtcacag cctcttattt
    54841 gttgaagatg cccatttctg taacaatctc ttgatacacg tcaggctcac aacctcactt
    54901 attttggaca tctacgggtc cgatctcttt atttaaaaaa taagaaatga tggttcggat
    54961 aactaccaac gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat
    55021 gatcccagct gaaacaagaa tttccaaact tagaagtata tagcctccca acatcttttt
    55081 aatagcattg ttctcgctga ctcggtcgct ttgcactttt gtatggacaa ataaatattt
    55141 caaaaaatcc ttgattgata gacaaacacc ccaaaccaat aaacagatag caaagatatc
    55201 aagtcccagt acaagttttt cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat
    55261 cctacctctc ctcttctttg tttttctttg tcttctattt cctgattcaa gaaataagaa
    55321 atgactgttc gaatgataat aataagtgca agcttcaaaa tatctgtcca agtcggcttg
    55381 ataactgact cgatgatatc agcaacgact agtacttcca agcttaacaa aatataactt
    55441 gctagcagtt ttcttgtatc tgcattacga gtattacgtt ctgcatagtt tttcttgaca
    55501 tgagaccaat caaaaagttt gaatagaaca agaacgatcc cgacaattaa gataccgata
    55561 gaacaaatat ttaaaatagc aatgatccat tctagaaatt caattaaatt tgagcgaaac
    55621 atatacgcta cctactttct tttaatttgt tcctattctt ttattgtaaa agaaagttac
    55681 cttgttcgtc tatttaaaag ttgttctaat gataacggtt cgcttaaatt tttctttttc
    55741 actctcccat ttttctccct ccatccataa atcgtggaag tatccaaaac aacgtagcaa
    55801 gttaaaataa ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt
    55861 ttgtacccaa atttctaaaa atagaaccat acacctcctc aaatcttttc ttcttaccta
    55921 ccctattttt atttacagga aaatcagaca cttcttcttt tttgtcaaaa tttccttttt
    55981 cttctcgtcc tatcatttgg cattctaata aaaacgaact atccttggat atagaaagag
    56041 aaaacaagga ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt
    56101 tgcttgccct tggcgctgca tatctaggaa aaaagacagg actattcgaa gatgacagtc
    56161 atttatatga tgaattcgaa tcaatctagc atcctattta gttgaatcac tacggacata
    56221 cttggaagac attggaggaa tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg
    56281 tattgccaat atggcagcgg ctgtttattt gattcaagaa ggcaaatgga aaggggatca
    56341 gatcaccttt tattcattgg atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt
    56401 agtggatgaa tattggaata aaaaccatcc attagaaaac caaaaaggct atattgctcg
    56461 aggcggtcgc atgcttaatt accgtaccta tgtagatttg atggatctgt taagtcgaat
    56521 accttctgtt actgagccag ggatgactgc tgaagaagat acgagagatt ttgatgcaaa
    56581 acaccgaacc tttgacaaag cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg
    56641 aaaactggga ttcaataata aagaccgtat tcttctgact aaactgattg ctatgcctga
    56701 ttcagaagaa gaaaaactag acaatatcac tatcgaagaa tatttcaaag atgatccaca
    56761 cttttttgaa acaaacttct ggtttatgtg ggaaacaaca tttgctttca gaacacgtag
    56821 ttctgctcaa gaattgcgtc gctatatgca tcaaatgatt tatgaattca ctcaaatcga
    56881 acatctagtg ggcgtcaatc gaacacgtta caatcaatac gaaagtatta tgcttccttt
    56941 gatcgagtac ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg tcactgactg
    57001 ggaattcaaa gaaacaccga tgcaagatga aatcactgtg actggtttga aaattaccaa
    57061 tacactgact caagaagaag aatacgttgt ggtcgatgaa gatacagcag ttattttcac
    57121 taatgggtcc attacagatt cagctacttt aggagattac gaaacaccag ctgtagaaaa
    57181 tatggattat ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt
    57241 gggaaatcct gacaaattct ttgctgatcg agatgcaagc gaatgggtca gctttaccct
    57301 gacgacgaaa aaccatttgt tgttgaatga aatcacccgt atcacgactc aagaaccagg
    57361 aaatgccttg aattcattta tttcaaccac accgatcacg ccgttaggcc aaaaagatgt
    57421 caatatgtct atcgtcgtgc atcatcaacc gcattttacg tctcagaaac caaatgaaac
    57481 agttatttgg ggttatttcc tttatccaag acgacgtggc gaatttgtgg ataaacagta
    57541 tattaaaatg aatggaaaag aaatgctaga agaactgatt ggacaacttt caaaagtaga
    57601 tcctggacca gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg taataaataa
    57661 tatccctgtc tatatgcctt atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg
    57721 accaaaagtc attcctaaac attcgaccaa tctggccttt acaggagaat ttgtcgagca
    57781 gccttatcag atgatcttta ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt
    57841 ccattttgcg ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat
    57901 tcctactcta gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc
    57961 tgccctgcat caagtaataa gaacaaccag gctaaaacag cttcttatgt ttttgatttg
    58021 gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttgtgaaaa
    58081 agctgccctg catcaagtaa taagaacacc caagctaaaa ctgcttctta tgtttttgac
    58141 ttggttggac ttattaccgc agatgcaagc ttttgaacac catttaccaa gaggttatga
    58201 aaaagctgcc ctgcatcaag taataagagc agccaagcta aaacagcttc ttatgttttt
    58261 gacttggtta gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
    58321 tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg tgaaaatggt tcctcacgtt
    58381 tttgcataaa tttgacttaa taccgagaaa ctgatccctt ctgtcataac ctctttttat
    58441 ttatgtctgt ttctttttga aaacaactat tttttcaacg tttactctct tgccaatcca
    58501 acatcatcta acggatacaa ctatttcaat gattaccatt acactaaaaa atcggaaaca
    58561 cacctctccg aacacaaata cataagacta tagaatgttt catttaagaa aaaaagcgtt
    58621 tcattatcaa aaaggtaaga tctcctttaa aatgaaatta tctgtagtac taataatgca
    58681 gacaatttta tgaatctgaa aggagcatct ccaatgaaag cagtcacttg gcaaggaaaa
    58741 cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat tagaaccaac agatgcaatc
    58801 atccgtatca cagcaacagc catttgcggc tccgatctgc atctttacca tcatggtgaa
    58861 gcagttttgg aaaaagggta tgttgtagga cacgaaccaa tgggaatcgt tgaagaagtg
    58921 ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg ttatcccttt taatattagt
    58981 tgtggtcatt gtcatttctg cctgaatcac atggaaagcc aatgtgatga atcaaatcca
    59041 gatcagttat atggcgggct tttcggattt ggcaagttga acggtaatca ttggggtggt
    59101 caagcggaat atctaagagt tccttatgca gactctactt catttgttgt accagataat
    59161 gatcttcctg atgaaaaagt tttatttcta tcagatattt tgcccacagc ttattggagc
    59221 gttgaaaacg caggagtaaa aaaaggcgat acagttgtga ttctaggttc tggtccagtc
    59281 ggtctattcg ctcaaaaatt cgctgttatg gctggagcag agcgcgtaat cgcagtagat
    59341 ccagtacagc accgtctaga taaagcggct aattacaatg gggttgaaac ttatttctta
    59401 gaagacacgg cacaagctgg aacagaattg tatgaactgg ctaaaggtgg cgcagatgtc
    59461 attatcgatt gtgtaggaat ggacggatta gagcctgtca aagaaaaagc gaaaaattta
    59521 gtaagcttac aaagcggcac aatctctcca ttacagatgg catcgcaagc tgtcaaaaaa
    59581 tttggaacag tccaagtaac tggtgtttat atgactccag cttcttctta cccgctgcaa
    59641 gaatttttta tgcgggatat cgctgtcaag catggacagg caccaattat ccatctaatg
    59701 ccaaaaattt acgcaatgat tgcagaagga ctattcgatc ctacacaaat cattactcat
    59761 tcgctgccac tatcagaagc agcacaagca tatgagatct ttgacaaaaa agaagataaa
    59821 aatataaaag ttgttttgaa accttgaatt cagtaaaaaa tacaggtgat tttctttatg
    59881 cccaaaatca aacgaaatac ccatgagatc gaaataggaa aagaagaaga tatcgaaata
    59941 cgaggacgtc gcttccgctt ctatttccaa aatcgttaca cattgatctc actggctgtc
    60001 gatttattaa ccgggatttt ctatattttt ggaagccttg cttccttaac taaaatcccc
    60061 gatcgatatg gcacctattt ttatttggct ggtgctattt tcttgacgat tcgtcctatt
    60121 ctcagaatcg taaggaacat ttttatttac gacgaaaaaa aagcaatgaa gagaacaagc
    60181 aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct cttttttcac cctatcgtat
    60241 cactttgata cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc
    60301 actcgtatcg ttcataaaac gtggtatgat ctgtaattaa gtaaagtttt tcgtacccaa
    60361 gttgatgcat ttcttttctg atttccgcta atatccgctt ggctatcccc tgcttacgct
    60421 tttccggttc aacaaacagg gcacaaagat ttggtgtcag atcagggcgg ttatggaagt
    60481 cgttttcaat gacgcccgct cctccgacga tggtttcttt ttcatctaag accacatacc
    60541 attgaggaat actttcctta ttagcgattc cagtacgaat actttcctca tacacaacag
    60601 caggaactcc ccattttgat gaaaaccaat tcgcgctttg ttttactaat tctgggtgtt
    60661 ctcttaattt gatccagtta tatgtaacca atgtttaaac ctccgactaa aataaaacgt
    60721 ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga caacaatagt gatgtaaaaa
    60781 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt gatcaagcat ctaaagcttc
    60841 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
    60901 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
    60961 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
    61021 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
    61081 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaaggacac
    61141 ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat
    61201 aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa
    61261 agaaaggttt gttcacgata aacggtgcgc tatgcccagc tgcgattgca ttaacgtttg
    61321 cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta
    61381 aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt
    61441 ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa
    61501 caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg
    61561 gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga
    61621 tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa
    61681 tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc
    61741 ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag
    61801 caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc
    61861 cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa
    61921 tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca
    61981 taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta
    62041 taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact
    62101 gtagattacg cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa
    62161 gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga
    62221 tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat
    62281 tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca
    62341 atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct
    62401 tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat
    62461 tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt
    62521 gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa
    62581 gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc
    62641 atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg
    62701 catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt
    62761 cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg
    62821 ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg
    62881 catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt
    62941 tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa
    63001 acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg
    63061 ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa
    63121 taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc
    63181 ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat
    63241 agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag
    63301 attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat
    63361 tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc
    63421 aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga
    63481 tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca tattctcttc
    63541 ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt agatacatgg
    63601 ctatatcata catgattttc ggatagattt cccatcccct ggaattattc attttcttcc
    63661 caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt
    63721 gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg
    63781 tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt
    63841 gacacaactc caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc
    63901 gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag
    63961 catagaccat ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt
    64021 aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt
    64081 ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga
    64141 ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt
    64201 gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca
    64261 aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga
    64321 tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact atacagttct
    64381 cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg
    64441 atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc
    64501 catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct
    64561 gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa
    64621 taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt
    64681 tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa
    64741 acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc attgacgatc
    64801 ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca
    64861 aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga
    64921 ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca
    64981 agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc
    65041 agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata
    65101 gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc
    65161 gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc
    65221 caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct
    65281 tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct
    65341 ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca
    65401 gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc
    65461 tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat
    65521 aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt
    65581 tcattcacta attcataaac agttggatta acagataaat aagacatcac tgcgatgata
    65641 ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca
    65701 gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat
    65761 tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt
    65821 gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg
    65881 ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca
    65941 ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga
    66001 cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact
    66061 cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc
    66121 agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta
    66181 ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct
    66241 gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt
    66301 tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc
    66361 cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc
    66421 cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct
    66481 tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa
    66541 ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg
    66601 caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc
    66661 tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg
    66721 acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc
    66781 taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct
    66841 ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt
    66901 ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca
    66961 taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa
    67021 aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt
    67081 ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc
    67141 tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact
    67201 gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg
    67261 caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga
    67321 ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg
    67381 ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg
    67441 gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta
    67501 gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc
    67561 tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat
    67621 ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg
    67681 tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga
    67741 gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt
    67801 cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat
    67861 cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat
    67921 tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat
    67981 tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt
    68041 tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa
    68101 tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta
    68161 gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc
    68221 catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta
    68281 taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta
    68341 ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc
    68401 tgttttgatt gttccgctaa ttctttttgt cgttcgcctt gataaaaaat cactggacga
    68461 tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa
    68521 tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct
    68581 tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg
    68641 cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata
    68701 caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca
    68761 tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag
    68821 tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt
    68881 tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat
    68941 acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg
    69001 aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt
    69061 agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct acagattttt
    69121 cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata
    69181 aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca
    69241 gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct tactttaata
    69301 tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt
    69361 acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt
    69421 acttggctct tctcattagt tactctatat atgatagaac taccagttgt tttataagga
    69481 aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc
    69541 aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc
    69601 tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa
    69661 ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat
    69721 agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa
    69781 gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta
    69841 ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca
    69901 taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa
    69961 gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc
    70021 agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt
    70081 ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc
    70141 attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct
    70201 ttagaaacca aacgaataaa aatgataatt aatgctgtac tcattaatgt tgatagtaca
    70261 tggctgtaca ctagcaaact catactcaat gctagcttta cagacggata cttttcttct
    70321 tttccagaga tgatttgata agcataaaat aatacaggga taacaagtgt tattgcgata
    70381 gcttcggcaa ttgcggaacg atagtagatg tctactgtcc gataagcaga gaaaatatag
    70441 agacaactaa ataaaaatgc cgcttttttt tgttttaaaa accgctcgcc ataatgataa
    70501 gctacgagtg ttgtacacaa gttcaataac caaacgtaaa taatatagct tacaatcaaa
    70561 ttttttgtca tccaatataa cattacagct ggaaaaaaag tcagaaaggg ataaaaataa
    70621 tttacaccat atccagtata attgaatgtc tcataattaa tcggactggt aaaaatagtc
    70681 gataacccct tgattcgaga taagtgaaaa gaaagatctc cagtggtata aatactctgt
    70741 gtatttttga aaaagatgaa agaatatata ctactaacta taattaatcc actcaatatt
    70801 actacttttt tatttatttt cattcttctt gtttatctcc tttttagtca catatcgaga
    70861 atctttcaac ttattaggtg caaatcgttt aaaacttgaa tacaccacta aacaataagt
    70921 cagaatactg atccataaag ccatgagagc agttggagta tattttgttg taatttctat
    70981 cttattttct ccagcattta catccacatt cactaagcca tgttttttat aagagtcatt
    71041 tacgacttta ccgtttactt ttacctgtgt acctaaataa gcatatactg gtactgtcac
    71101 tttttgcttt ttgttgcttg ccactgtgta agtaatgtta ttatctgcag ctttatactt
    71161 ccctttttcc cacgattctc ctacttttac ttcatgtttt tctaagtgag gcctttcttt
    71221 tatcgcttct gttggggcgt aatcgtataa tcctcccata tctgatgttg tataacgtgg
    71281 gagtgtttct tcattgataa caatatgacc gtgttttgat tggataactt ttgcgtaatt
    71341 ttcttttgct gctatagacg tgacgagtag aaacaccaca gtagcgccaa tgattaacca
    71401 tgaatatttt ttattcatag tctttttcat aaaaacaagc gttatgatca ttgaagaaaa
    71461 aaagatttgg atacctaaga tacgccaagg aaactggagc aagcttatcg gtgttttttg
    71521 aaacaaaaac catggaaata aattagttac gcatacccat gtagaaacta cgccaacata
    71581 gatgattttt tcttttttct ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac
    71641 caatcctaat aaccccatag tgtatgttgt aatatcgtta tttaacgctt gaatgatact
    71701 atctgaaata gaaagtgctt ctagctgcaa atctgttttg aagggactgt gtatttcttg
    71761 ataattcatc tgctgcaaca taggatacca aaaatagcta gttaatgcac aagtcaaaaa
    71821 aacagccttt actaaattca cgaccacaaa ttttatatcg ttttttattt gcttgcttaa
    71881 aaacaaacga actataatca tacaagcaat caatgcacta cacaatagtg tagttaagac
    71941 atgagtatac acaagtagac ttatactgac agataaagcg atgacttttt tttctctatt
    72001 gaatatcaat ccataagcat aatataaaac agctgggata atcgtaagag cgattgcttc
    72061 tgcaattgcg gatcgataat agatatcaac tgttcgataa gttgcaaaag tatataaaca
    72121 actaaataaa aaagcagcat ctatcttttt aaaaaacttt tctccataat aaaaact
//
LOCUS       DACEKQ010000003        63715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEKQ010000003 DACEKQ010000000
VERSION     DACEKQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.003"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..122)
                     /locus_tag="IW671_000138"
     CDS             complement(6..122)
                     /locus_tag="IW671_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0897156.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(195..1151)
                     /locus_tag="IW671_000139"
     CDS             complement(195..1151)
                     /locus_tag="IW671_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0897157.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1322..1690
                     /locus_tag="IW671_000140"
     CDS             1322..1690
                     /locus_tag="IW671_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0897158.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1807..2292
                     /gene="mraZ"
                     /locus_tag="IW671_000141"
     CDS             1807..2292
                     /gene="mraZ"
                     /locus_tag="IW671_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0897159.1"
                     /translation="MGDCGRLILVSGGMGGLSMLMGEFQHNIDAKGRLIVPSKLREEL
                     GEKFVLTRGLDGCLFGYPMSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQ
                     GRINIPSTLRNYAALTKGCVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFG
                     L"
     gene            2313..3272
                     /gene="rsmH"
                     /locus_tag="IW671_000142"
     CDS             2313..3272
                     /gene="rsmH"
                     /locus_tag="IW671_000142"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0897160.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3277..3678
                     /gene="ftsL"
                     /locus_tag="IW671_000143"
     CDS             3277..3678
                     /gene="ftsL"
                     /locus_tag="IW671_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0897161.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            3679..5871
                     /locus_tag="IW671_000144"
     CDS             3679..5871
                     /locus_tag="IW671_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0897162.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5897..6859
                     /locus_tag="IW671_000145"
     CDS             5897..6859
                     /locus_tag="IW671_000145"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0897163.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6873..8234
                     /locus_tag="IW671_000146"
     CDS             6873..8234
                     /locus_tag="IW671_000146"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0897164.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8260..9348
                     /gene="murG"
                     /locus_tag="IW671_000147"
     CDS             8260..9348
                     /gene="murG"
                     /locus_tag="IW671_000147"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0897165.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9363..10583
                     /locus_tag="IW671_000148"
     CDS             9363..10583
                     /locus_tag="IW671_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0897166.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10739..12064
                     /gene="ftsA"
                     /locus_tag="IW671_000149"
     CDS             10739..12064
                     /gene="ftsA"
                     /locus_tag="IW671_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0897167.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12087..13328
                     /gene="ftsZ"
                     /locus_tag="IW671_000150"
     CDS             12087..13328
                     /gene="ftsZ"
                     /locus_tag="IW671_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0897168.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEIGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13345..14022
                     /locus_tag="IW671_000151"
     CDS             13345..14022
                     /locus_tag="IW671_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0897169.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14036..14635
                     /locus_tag="IW671_000152"
     CDS             14036..14635
                     /locus_tag="IW671_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0897170.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14650..14937
                     /locus_tag="IW671_000153"
     CDS             14650..14937
                     /locus_tag="IW671_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0897171.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14986..15768
                     /locus_tag="IW671_000154"
     CDS             14986..15768
                     /locus_tag="IW671_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0897172.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15789..16496
                     /locus_tag="IW671_000155"
     CDS             15789..16496
                     /locus_tag="IW671_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0897173.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16758..19556
                     /gene="ileS"
                     /locus_tag="IW671_000156"
     CDS             16758..19556
                     /gene="ileS"
                     /locus_tag="IW671_000156"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0897174.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19605..21131)
                     /locus_tag="IW671_000157"
     CDS             complement(19605..21131)
                     /locus_tag="IW671_000157"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0897175.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21146..21793)
                     /locus_tag="IW671_000158"
     CDS             complement(21146..21793)
                     /locus_tag="IW671_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0897176.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21977..22306)
                     /locus_tag="IW671_000159"
     CDS             complement(21977..22306)
                     /locus_tag="IW671_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0897177.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22483..23211
                     /gene="sfsA"
                     /locus_tag="IW671_000160"
     CDS             22483..23211
                     /gene="sfsA"
                     /locus_tag="IW671_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0897178.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23227..24240
                     /locus_tag="IW671_000161"
     CDS             23227..24240
                     /locus_tag="IW671_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0897179.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24240..25517
                     /locus_tag="IW671_000162"
     CDS             24240..25517
                     /locus_tag="IW671_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0897180.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25580..28282
                     /locus_tag="IW671_000163"
     CDS             25580..28282
                     /locus_tag="IW671_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0897181.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28434..28751
                     /locus_tag="IW671_000164"
     CDS             28434..28751
                     /locus_tag="IW671_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897182.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28781..29101
                     /locus_tag="IW671_000165"
     CDS             28781..29101
                     /locus_tag="IW671_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0897183.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29209..30669
                     /locus_tag="IW671_000166"
     CDS             29209..30669
                     /locus_tag="IW671_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0897184.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30737..30958
                     /locus_tag="IW671_000167"
     CDS             30737..30958
                     /locus_tag="IW671_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897185.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30989..31171
                     /locus_tag="IW671_000168"
     CDS             30989..31171
                     /locus_tag="IW671_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0897186.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31171..31584
                     /locus_tag="IW671_000169"
     CDS             31171..31584
                     /locus_tag="IW671_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0897187.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31707..32888
                     /locus_tag="IW671_000170"
     CDS             31707..32888
                     /locus_tag="IW671_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897188.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            33277..33350
                     /locus_tag="IW671_000171"
     tRNA            33277..33350
                     /locus_tag="IW671_000171"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33311..33313,aa:Arg,seq:cct)
     gene            complement(33419..34558)
                     /locus_tag="IW671_000172"
     CDS             complement(33419..34558)
                     /locus_tag="IW671_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897189.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(34857..35492)
                     /locus_tag="IW671_000173"
     CDS             complement(34857..35492)
                     /locus_tag="IW671_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897190.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(35605..36240)
                     /locus_tag="IW671_000174"
     CDS             complement(35605..36240)
                     /locus_tag="IW671_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0897191.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(36274..36735)
                     /locus_tag="IW671_000175"
     CDS             complement(36274..36735)
                     /locus_tag="IW671_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0897192.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(36865..37296)
                     /locus_tag="IW671_000176"
     CDS             complement(36865..37296)
                     /locus_tag="IW671_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0897193.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(37314..37634)
                     /locus_tag="IW671_000177"
     CDS             complement(37314..37634)
                     /locus_tag="IW671_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897194.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            37933..38709
                     /locus_tag="IW671_000178"
     CDS             37933..38709
                     /locus_tag="IW671_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0897195.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            38724..38927
                     /locus_tag="IW671_000179"
     CDS             38724..38927
                     /locus_tag="IW671_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897196.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38943..39281
                     /locus_tag="IW671_000180"
     CDS             38943..39281
                     /locus_tag="IW671_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0897197.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            39268..39447
                     /locus_tag="IW671_000181"
     CDS             39268..39447
                     /locus_tag="IW671_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897198.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(39490..39960)
                     /locus_tag="IW671_000182"
     CDS             complement(39490..39960)
                     /locus_tag="IW671_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897199.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            40047..40745
                     /locus_tag="IW671_000183"
     CDS             40047..40745
                     /locus_tag="IW671_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0897200.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            40923..41264
                     /locus_tag="IW671_000184"
     CDS             40923..41264
                     /locus_tag="IW671_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897201.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            41257..41928
                     /locus_tag="IW671_000185"
     CDS             41257..41928
                     /locus_tag="IW671_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0897202.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            41934..42620
                     /locus_tag="IW671_000186"
     CDS             41934..42620
                     /locus_tag="IW671_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897203.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            42623..43372
                     /locus_tag="IW671_000187"
     CDS             42623..43372
                     /locus_tag="IW671_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897204.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            43384..43653
                     /locus_tag="IW671_000188"
     CDS             43384..43653
                     /locus_tag="IW671_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897205.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            43816..44115
                     /locus_tag="IW671_000189"
     CDS             43816..44115
                     /locus_tag="IW671_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897206.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            44115..44417
                     /locus_tag="IW671_000190"
     CDS             44115..44417
                     /locus_tag="IW671_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897207.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            44457..44822
                     /locus_tag="IW671_000191"
     CDS             44457..44822
                     /locus_tag="IW671_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897208.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            45449..45637
                     /locus_tag="IW671_000192"
     CDS             45449..45637
                     /locus_tag="IW671_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897209.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            45645..45839
                     /locus_tag="IW671_000193"
     CDS             45645..45839
                     /locus_tag="IW671_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897210.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(45872..46084)
                     /locus_tag="IW671_000194"
     CDS             complement(45872..46084)
                     /locus_tag="IW671_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897211.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            46209..46412
                     /locus_tag="IW671_000195"
     CDS             46209..46412
                     /locus_tag="IW671_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897212.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            46409..46681
                     /locus_tag="IW671_000196"
     CDS             46409..46681
                     /locus_tag="IW671_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ0897213.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            46682..46867
                     /locus_tag="IW671_000197"
     CDS             46682..46867
                     /locus_tag="IW671_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897214.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            46854..47201
                     /locus_tag="IW671_000198"
     CDS             46854..47201
                     /locus_tag="IW671_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897215.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            47161..47637
                     /locus_tag="IW671_000199"
     CDS             47161..47637
                     /locus_tag="IW671_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ0897216.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(47783..48511)
                     /locus_tag="IW671_000200"
     CDS             complement(47783..48511)
                     /locus_tag="IW671_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ0897217.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            48559..48837
                     /locus_tag="IW671_000201"
     CDS             48559..48837
                     /locus_tag="IW671_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897218.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            48839..49225
                     /locus_tag="IW671_000202"
     CDS             48839..49225
                     /locus_tag="IW671_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897219.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            49222..49602
                     /locus_tag="IW671_000203"
     CDS             49222..49602
                     /locus_tag="IW671_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0897220.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            49714..50166
                     /locus_tag="IW671_000204"
     CDS             49714..50166
                     /locus_tag="IW671_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ0897221.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            50163..51890
                     /locus_tag="IW671_000205"
     CDS             50163..51890
                     /locus_tag="IW671_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0897222.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            51954..53141
                     /locus_tag="IW671_000206"
     CDS             51954..53141
                     /locus_tag="IW671_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0897223.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            53107..53682
                     /locus_tag="IW671_000207"
     CDS             53107..53682
                     /locus_tag="IW671_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ0897224.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            53695..55059
                     /locus_tag="IW671_000208"
     CDS             53695..55059
                     /locus_tag="IW671_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0897225.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            55061..55342
                     /locus_tag="IW671_000209"
     CDS             55061..55342
                     /locus_tag="IW671_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0897226.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            55320..55646
                     /locus_tag="IW671_000210"
     CDS             55320..55646
                     /locus_tag="IW671_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0897227.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            55636..55974
                     /locus_tag="IW671_000211"
     CDS             55636..55974
                     /locus_tag="IW671_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897228.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            55964..56329
                     /locus_tag="IW671_000212"
     CDS             55964..56329
                     /locus_tag="IW671_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0897229.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            56336..56962
                     /locus_tag="IW671_000213"
     CDS             56336..56962
                     /locus_tag="IW671_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0897230.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            56962..57426
                     /locus_tag="IW671_000214"
     CDS             56962..57426
                     /locus_tag="IW671_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897231.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            57621..59930
                     /locus_tag="IW671_000215"
     CDS             57621..59930
                     /locus_tag="IW671_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0897232.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            59942..60646
                     /locus_tag="IW671_000216"
     CDS             59942..60646
                     /locus_tag="IW671_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0897233.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            60643..63402
                     /locus_tag="IW671_000217"
     CDS             60643..63402
                     /locus_tag="IW671_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0897234.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            63415..>63715
                     /locus_tag="IW671_000218"
     CDS             63415..>63715
                     /locus_tag="IW671_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0897235.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKG"
BASE COUNT        21720 a        10437 c        13149 g        18409 t
ORIGIN      
        1 tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc
       61 attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc
      121 attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc
      181 acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa
      241 ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac
      301 ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa
      361 gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga
      421 ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc
      481 accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag tttattttgc
      541 gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt
      601 ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct
      661 tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc
      721 tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc
      781 tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt
      841 ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt
      901 gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg
      961 aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga
     1021 atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac
     1081 cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa
     1141 tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg
     1201 gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta aataaaatga
     1261 aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt
     1321 catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt
     1381 tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc
     1441 tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac
     1501 aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt
     1561 tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg
     1621 tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc
     1681 ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg
     1741 atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg
     1801 tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg ggggctttca
     1861 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
     1921 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
     1981 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
     2041 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
     2101 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
     2161 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
     2221 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
     2281 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
     2341 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
     2401 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
     2461 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
     2521 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
     2581 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
     2641 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
     2701 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
     2761 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
     2821 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
     2881 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
     2941 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
     3001 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
     3061 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
     3121 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
     3181 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
     3241 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
     3301 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
     3361 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
     3421 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
     3481 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
     3541 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
     3601 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
     3661 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
     3721 aatctaaatc cgataaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
     3781 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
     3841 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
     3901 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
     3961 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
     4021 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
     4081 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
     4141 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
     4201 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
     4261 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
     4321 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
     4381 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
     4441 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
     4501 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
     4561 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
     4621 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
     4681 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
     4741 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
     4801 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
     4861 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
     4921 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
     4981 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
     5041 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
     5101 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
     5161 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
     5221 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
     5281 gcaaaaaccg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
     5341 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
     5401 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
     5461 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
     5521 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
     5581 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
     5641 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
     5701 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
     5761 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
     5821 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
     5881 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
     5941 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
     6001 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
     6061 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
     6121 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
     6181 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
     6241 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
     6301 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
     6361 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
     6421 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
     6481 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
     6541 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
     6601 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
     6661 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
     6721 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
     6781 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
     6841 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
     6901 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
     6961 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
     7021 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
     7081 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
     7141 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
     7201 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
     7261 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
     7321 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
     7381 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
     7441 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
     7501 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
     7561 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
     7621 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
     7681 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
     7741 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
     7801 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
     7861 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
     7921 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
     7981 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
     8041 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
     8101 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
     8161 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
     8221 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
     8281 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
     8341 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
     8401 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
     8461 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
     8521 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
     8581 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
     8641 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
     8701 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
     8761 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
     8821 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
     8881 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
     8941 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
     9001 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
     9061 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
     9121 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
     9181 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
     9241 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
     9301 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
     9361 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
     9421 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
     9481 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
     9541 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
     9601 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
     9661 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
     9721 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
     9781 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
     9841 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
     9901 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
     9961 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    10021 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    10081 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    10141 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    10201 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    10261 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    10321 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    10381 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    10441 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    10501 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    10561 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    10621 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    10681 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    10741 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    10801 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    10861 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    10921 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    10981 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    11041 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    11101 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    11161 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    11221 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    11281 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    11341 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    11401 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    11461 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    11521 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    11581 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    11641 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    11701 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    11761 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    11821 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    11881 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    11941 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    12001 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    12061 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    12121 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    12181 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    12241 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    12301 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    12361 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    12421 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    12481 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    12541 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    12601 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    12661 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    12721 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    12781 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    12841 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    12901 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    12961 atatcattct aggtacatct atcaatgaag aaataggcga tgaaatccgt gtgacggtca
    13021 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    13081 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    13141 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    13201 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    13261 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    13321 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    13381 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    13441 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    13501 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    13561 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    13621 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    13681 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    13741 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    13801 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    13861 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    13921 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    13981 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    14041 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    14101 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    14161 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    14221 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    14281 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    14341 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    14401 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    14461 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    14521 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    14581 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    14641 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    14701 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    14761 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    14821 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    14881 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    14941 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    15001 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    15061 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    15121 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    15181 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    15241 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    15301 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    15361 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    15421 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    15481 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    15541 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    15601 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    15661 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    15721 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    15781 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    15841 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    15901 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    15961 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    16021 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    16081 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    16141 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    16201 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    16261 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    16321 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    16381 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    16441 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    16501 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    16561 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    16621 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    16681 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    16741 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    16801 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    16861 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    16921 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    16981 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    17041 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    17101 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    17161 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    17221 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    17281 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    17341 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    17401 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    17461 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    17521 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    17581 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    17641 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    17701 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    17761 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    17821 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    17881 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    17941 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    18001 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    18061 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    18121 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    18181 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    18241 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    18301 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    18361 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    18421 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    18481 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    18541 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    18601 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    18661 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    18721 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    18781 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    18841 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    18901 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    18961 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    19021 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    19081 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    19141 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    19201 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    19261 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    19321 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    19381 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    19441 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    19501 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    19561 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    19621 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    19681 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    19741 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    19801 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    19861 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    19921 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    19981 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    20041 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    20101 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    20161 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    20221 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    20281 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    20341 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    20401 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    20461 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    20521 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    20581 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    20641 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    20701 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    20761 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    20821 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    20881 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    20941 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    21001 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    21061 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    21121 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    21181 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    21241 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    21301 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    21361 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    21421 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    21481 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    21541 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    21601 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    21661 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    21721 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    21781 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    21841 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    21901 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    21961 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    22021 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    22081 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    22141 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    22201 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    22261 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    22321 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    22381 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    22441 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    22501 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    22561 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    22621 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    22681 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    22741 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    22801 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    22861 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    22921 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    22981 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    23041 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    23101 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    23161 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    23221 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    23281 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    23341 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    23401 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    23461 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    23521 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    23581 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    23641 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    23701 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    23761 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    23821 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    23881 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    23941 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    24001 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    24061 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    24121 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    24181 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    24241 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    24301 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    24361 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    24421 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    24481 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    24541 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    24601 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    24661 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    24721 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    24781 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    24841 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    24901 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    24961 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    25021 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    25081 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    25141 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    25201 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    25261 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    25321 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    25381 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    25441 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    25501 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    25561 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    25621 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    25681 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    25741 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    25801 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    25861 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    25921 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    25981 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    26041 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    26101 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    26161 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    26221 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    26281 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    26341 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    26401 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    26461 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    26521 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    26581 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    26641 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    26701 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    26761 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    26821 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    26881 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    26941 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    27001 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    27061 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    27121 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    27181 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    27241 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    27301 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    27361 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    27421 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    27481 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    27541 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    27601 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    27661 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    27721 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    27781 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    27841 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    27901 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    27961 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    28021 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    28081 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    28141 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    28201 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    28261 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    28321 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    28381 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    28441 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    28501 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    28561 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    28621 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    28681 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    28741 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    28801 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    28861 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    28921 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    28981 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    29041 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    29101 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    29161 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    29221 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    29281 ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc
    29341 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    29401 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    29461 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    29521 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    29581 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    29641 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    29701 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    29761 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    29821 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    29881 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    29941 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta
    30001 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    30061 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    30121 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    30181 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    30241 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    30301 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    30361 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    30421 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    30481 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    30541 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    30601 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    30661 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    30721 tctaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    30781 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    30841 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    30901 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    30961 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    31021 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    31081 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    31141 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    31201 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    31261 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    31321 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    31381 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    31441 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    31501 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    31561 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    31621 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    31681 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    31741 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    31801 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    31861 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    31921 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    31981 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    32041 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    32101 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    32161 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    32221 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    32281 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    32341 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    32401 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    32461 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    32521 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    32581 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    32641 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    32701 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    32761 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    32821 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    32881 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    32941 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    33001 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    33061 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    33121 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    33181 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    33241 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    33301 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac
    33361 ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact
    33421 agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt
    33481 caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta
    33541 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat
    33601 gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca
    33661 aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat
    33721 tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt
    33781 ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg
    33841 tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt
    33901 cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac
    33961 cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt
    34021 tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat
    34081 cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg
    34141 catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt
    34201 ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata
    34261 ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag
    34321 gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata
    34381 gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat
    34441 cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat
    34501 cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa
    34561 ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt
    34621 ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt
    34681 aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt
    34741 gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt
    34801 ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa
    34861 gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt
    34921 ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat
    34981 aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca
    35041 gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt
    35101 aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgct gttaattcga
    35161 agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt
    35221 tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg
    35281 aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag
    35341 aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca
    35401 acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct
    35461 tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt catttccttt
    35521 acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct
    35581 aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca
    35641 ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac
    35701 tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga
    35761 aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat
    35821 aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc
    35881 actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact
    35941 atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag
    36001 gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt
    36061 gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat
    36121 cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat
    36181 taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat
    36241 taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt
    36301 aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat actttttaat
    36361 ttcatctgca cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt
    36421 ctctatatat ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt
    36481 gttttcatct ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt
    36541 aaattgtata taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt
    36601 aaaggggata gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct
    36661 aataattgaa atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa
    36721 aatctctttt tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc
    36781 atatatgagg aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg
    36841 tttttttaat aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt
    36901 aaaatagcat tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata
    36961 ctccataaat ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat
    37021 atttaataaa attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt
    37081 tgtagaagta tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc
    37141 atgagataaa tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat
    37201 tctgggtttt ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt
    37261 gtattcgctc aacatttctt tcaatttcaa atacatacaa accatcactc caactattca
    37321 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    37381 tttccatcaa aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct
    37441 ctttcttctc tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt
    37501 aattcatctg aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca
    37561 aaattaattt ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt
    37621 tcataggtat tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa
    37681 tacgcagaaa taatttgact aattctgagt aatgagctat actatatctt gtaaacagat
    37741 ttaacaacta aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa
    37801 gctttaaaat caatgttttt atgtcttatt taattatgct cttagtatag agtattactc
    37861 agatactgtc aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    37921 ggaatgagaa atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca
    37981 attttagtaa atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat
    38041 tcaaacacta aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga
    38101 atcacgacct caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt
    38161 ttaatcttaa aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa
    38221 gtgttgccag caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct
    38281 ttgcttaatc cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga
    38341 agattaatcg cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc
    38401 atattatctt ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct
    38461 ccacaacaga tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa
    38521 tggttattgt gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc
    38581 ccgaaagccg atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt
    38641 ctatttatct acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa
    38701 attggttaga aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag
    38761 aaaaaatcaa agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa
    38821 gatttggaat tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag
    38881 aagcaaacag aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc
    38941 taatgcaata tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag
    39001 tggattatga ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag
    39061 tgctagaacg gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa
    39121 gatatagaaa tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg
    39181 aaggaagaga cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt
    39241 tttcggaaat cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc
    39301 gcaccgctaa tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg
    39361 attgtttaca tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg
    39421 aaaaagccaa ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat
    39481 tactattact cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat
    39541 atcaacaatt ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt
    39601 aaggagttta ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact
    39661 tttaggagga atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc
    39721 tcctttagca attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga
    39781 tgaaagttct aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt
    39841 tgtataaatc atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg
    39901 cattcctttt atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat
    39961 ccattgaaaa attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa
    40021 ttataccaaa aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag
    40081 ctgtgactac gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa
    40141 gagatattga tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta
    40201 atgaaccaga ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt
    40261 tcttgctggc tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg
    40321 aagcattcaa cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag
    40381 atatgatgat ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta
    40441 aagatactat gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga
    40501 aagttatggg agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag
    40561 tattctcaaa attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt
    40621 tacctcgtaa gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg
    40681 cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg
    40741 aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat
    40801 aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa
    40861 aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc
    40921 atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc
    40981 cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac
    41041 tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta
    41101 tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga
    41161 ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat tacggaccag
    41221 gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg
    41281 aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt
    41341 gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg
    41401 gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt
    41461 gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta
    41521 acggttaaag gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca
    41581 gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca
    41641 ttggctctac acggactggg attatatgta tttcaagggg aagatattcc aacaccacct
    41701 agaatcgata caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag
    41761 atgggtaaag atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta
    41821 gggctcttag ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg
    41881 atggagcgag caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta
    41941 aaccattaat cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg
    42001 caaccatcaa tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg
    42061 gtgatcgagt cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa
    42121 acttcatcta tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct
    42181 ggaaggaatt cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag
    42241 acgaatcgaa tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca
    42301 tctttgaaca tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat
    42361 attacttcta caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg
    42421 acatcgatca ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag
    42481 agtacacatt tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga
    42541 ttaatttcaa aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa
    42601 agttaaatat tggagggtaa aaatgacaga acatcgaagt tattatgcga ttataccagc
    42661 caacgtaagg tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac
    42721 ggccttgtgt aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata
    42781 tggtgtgaat aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt
    42841 gaatcgggaa atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat
    42901 taatcaatac cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa
    42961 taatacatct attaataata catttaataa tacaaaagaa tatataagag agttaccgcc
    43021 ttcgaaaaaa tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct
    43081 tttgtcagat gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg
    43141 aatcgaacga ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc
    43201 aactattcga agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata
    43261 caagcgcaca ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct
    43321 caagaaaaaa gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga
    43381 aaaatgaaga tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag
    43441 gaattagacg tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt
    43501 aagaatgatc gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga
    43561 acgcaattaa gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg
    43621 caaaaacatc ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag
    43681 cacagatcga attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt
    43741 ggctcaacac atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga
    43801 aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat
    43861 tcagaagatt ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca
    43921 atgtggactg aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat
    43981 cgaattctag taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat
    44041 attatttggg atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat
    44101 gtggaggaga aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg
    44161 gattggatca agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag
    44221 ttgccgcttc tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag
    44281 tagtaacctt gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt
    44341 ttgcttatga cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt
    44401 ccagtgacct gaaataattc cattaatgga agaaataact gaataggagg atagacatga
    44461 ttgatgcaaa acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc
    44521 taccatttct taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt
    44581 atgttgtaat tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta
    44641 ttaactgttg ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg
    44701 aatattgtcg tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc
    44761 taaacatcaa cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct
    44821 aaccaccaat atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt
    44881 aaggttcttt acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat
    44941 ccaaagctct cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag
    45001 aacctgagag ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt
    45061 gtaaacacat aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt
    45121 cagtaggaaa aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc
    45181 tgcaattaaa ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag
    45241 aaagtcaagc agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg
    45301 aaggcgatag tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg
    45361 atcaaatcga attagctaga caatactttg ttccatatgc agatacctgg tccaagcata
    45421 agaatgacat catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga
    45481 ggaagccaaa tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca
    45541 aggagttaag ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt
    45601 gcttttgctc catctagcag aaaaaaattt gatttgaggt gaaaatgatg attcaacttg
    45661 caggcatcca aacagggaaa atttattttt ctggagaaag caaaagcgaa gccaaccaat
    45721 ggctattgaa aacttacacg aacaataaga agctaaggaa aaaatatcct gatttgttgc
    45781 taaaggatga tcagattatg ccggaaccga tgattttgac ccgtaaggag ggaagttgaa
    45841 actaaaaaac cagccgtgtg ggctggagtt gctatttctt tttaataatg agtttttcta
    45901 aatttaattc attctcacga gtttgcgtcc aataatcaga ataatatttt aattgtttaa
    45961 gagtaggaat gtcgtcttcg gtaaaattag aaaataattc tggagaatta tcttttttta
    46021 attgttctaa tcctgattct ttaaaacgtt gttcaacgag ttttactaaa ttgttgatat
    46081 tcatgataaa tcaccttttt aaatttaatt tcgatttgat taatcgatac aagtattata
    46141 tcaaataaaa aagctgattt agggggtctt acaaaaacac taaaattttt tcaaaaagag
    46201 gaggaacggt ggtgaaagtc agagaacaat tcaagatagt gagcgacgaa gataatgttt
    46261 acaaaataaa gatcgttaat attaatgaat atcgagaagc agatcacaaa tatttggttg
    46321 acttatacat gaacgataaa tatttgggag aaggattttg tggagattca ctttttgatg
    46381 atgagcgaat tttgaaggag gaaacagaat gatccacgaa ctaaaaatac taccagaata
    46441 ttttgaagca gtgacgagcg gacgcaaaca attcgagatc cgcaagaatg atcgtgactt
    46501 caaagttggt gatcagttaa tactacgtga atataaaaaa tacattaaac atgaattacc
    46561 tggactaacg agaccaacat ttagttacac tggtgactca tacaaagcag agatcactta
    46621 tattactgat tacatgcaga agtccggata tgtagtgttg gggattaaag gggtgaatta
    46681 aatgaaagta tacatcttag ctatcacaga gggcacatgg atgttcccag tcggttccgg
    46741 aaaaatctat aaatcaaaaa cagctgctta taaagcattt gaaaaataca aaaaagaaaa
    46801 cggtggcggc actaacgcaa agattctagt ggctgataac tggcatgaag aaggtgaacg
    46861 taattgattt acctgttcat tttcgtggta ctaactattt ttatgctagt aatcttattc
    46921 aaaaaaagat gagtaaagac gaacgaaaaa ttctggccaa cttttattta gtattaaaaa
    46981 tcgaacaaaa tgtgttttcg gaaaggagaa ttgtatggga aaaacaaaat caaagattaa
    47041 gaagaaaaaa cgacgtttgg aacaaaaggc aatccagaat ggaacggcta agaaaaaata
    47101 aaaaagctac ccctttcgag atagcagcgc aatactattc taccataagg ggtggcgttt
    47161 gtgagatttc attggttaaa agattaccaa gaactagatg agcagattct ttacttaaag
    47221 tggaatctta ataaaagtaa gcttgaattg aatcgatggg tctgtggtga tttagcaaac
    47281 gtccgcatcg aaaagaattc gagaacttct tctttagaag agaatatcca aaagatagaa
    47341 aatgaattgg aattgttgat cgagcaaaaa gaagaaatgt tattgctgat agatagcttt
    47401 tctggtatcg ataatcaaat tgttaagatg aaatatgttg atcaaatgag cttagaagat
    47461 attgctgaat ctgttgggta tagctcatcg tatgtcagac agcgacatgc agaaattaga
    47521 aagacattga actttttaga tgagtatgaa cacagacaag ctgaccgatt gaaaaaagaa
    47581 aacgaaattg atttttacaa tagcgagaag tacaaagaac aactctcttt attctaaaat
    47641 tacactgttc actaaatgtt cggtcattgt agctatgtaa acattgttag cagcatgata
    47701 ttctattagt gtcaaaaaaa tatgaaagag ccaagatatc ccaaccgttt tattaattgg
    47761 tatctgtggc tcttttattc ttttaattag tagttggcat aaagttattt ccatttataa
    47821 attgatcagc taaatcagca tcataatcag ccgcgataaa ttgcgctgct gatttaataa
    47881 atgcatccat atcatgaaca tctttattag tatgtctacg aacatagtgc gtttcatcat
    47941 taccaatcca agtagcagct ttggctaatc tttggagttt aggaaaatca ttcaaataat
    48001 tttcaattgt tttacctaat aacatttttt ttattttatc ttcatcctct gaatttttag
    48061 atattgcata atctttaatt agaaattcta gagcttttct ataaccaact ccagcaattt
    48121 gttccaattg ctcagattta gcttgtgtgg cttgagtata aatatcaaca aatgtaggag
    48181 aaactttttc aatattttct ggtaaatcta ttttaatcgg cggacggtaa taatctttaa
    48241 ttaaaatatt ttcagaatat ccttcatctt cattaaacgt atttataaat tgtaaactat
    48301 aaaatttgtt gcactcatca tttatacact gtactaatac gccaaagatt ctatcattat
    48361 cgaacgcact atatgatgaa accccaaaat acacatacgg agtcattata tttccgcaat
    48421 gcggacaact atttggcaaa taaattgtct ctgtattttg acgatcacga tacttaatat
    48481 taacttcttg aagcattaaa acacctcgca tcttttttat aaatcatacc acaaaatatc
    48541 tgataggaga gtaaacaaat gaatgatttt catgaagcgg tacttagtat tgaagtagaa
    48601 tcaagtttgg ctaaagttta taagaaagca attgaagcag agaatagtcc gtacagagaa
    48661 aattggaatg gtaatcatgc acatgtgcaa gtggagaatg atgattacag aactggaatg
    48721 aatacgttag ttatttcttt attgtcacac actctaccca atctgcagga aacgattgaa
    48781 tggtatgagc gaatgggcgc aaaagtaatc cgtacaaatt acaaaggaga gaattaaaat
    48841 ggcaatgatc aaaattagaa catccattag tggaacagag tattgggatt cagaaaagaa
    48901 aaggacagta gttgttccga aagatcaaga accaaatttt gaagtaaaag aagaaaaagg
    48961 aattttgagt gatggcaaat catttgttgc tgtcaatggc gaactcatag ctcatagcag
    49021 tgaaattctt gatagcgatg gaaacactgc tgctaatttt gatggagacg aagctactaa
    49081 tgatcagtcc actgaagata cggatgaact ggacaatatg actgcaaaag aattgcgtgc
    49141 atatgctaag aaacatggta ttgatattcc tggtgctatc cgtgcaaaag gcgatatcat
    49201 aaatcttatt cgtgaagtag aatgaaatac tgtcagtttg acggatgcac gaacaaaata
    49261 gcaaagggta tctactgtac tgaacacaag agatcaagca gatcacgcaa gaagaagcag
    49321 caagcaaagt ctgtttatca tcatgagaac aaaccattct atcgaacgca agcatggaaa
    49381 gatatgcgtc aatttattta tgaaagagaa ggtggtcatt gtcagcggtg tggtcagttc
    49441 atatttggaa agagagcaca cgtccatcac attgtaccaa tcaaagacaa cgaactgctt
    49501 aagcttgatc caaacaatct catgctatta tgttcaaaat gtcatccaat tgttgaaaac
    49561 gaaacggaag acaaaaaagt ttttccttcg tatttcaatt gaagcccccc tattcatttt
    49621 caaaattttt tcgcgtgggg agatagggta gcggggagtc acgcgcatcg ttaggtcaaa
    49681 tttttcaaaa aacaaagggg ggtgtataca aaaatgacga ctaaagcgca acgtaaagcg
    49741 attattgatg aaaaagtaag tgctgaaaaa gctcgtatct tagaaataat gaatttgtct
    49801 gatttgtaca ccatcactct tgatccatta atcgaatcat acttggatat ttttgaaatt
    49861 taccaacaca aatatttatt gtggaaggaa aaaggatttc cggagacgca aaaattcacg
    49921 aataaatcag gagctactaa tcaatcaaaa catccattgg cacaacaagt agaaacttgg
    49981 gcagataaga agatgaaagc tctagattta ttaggattaa caaacaaagc aaaaactggt
    50041 agactaattg ctggaggatc gaccgcaaga aaagatgaag aaattacacg cccagaagta
    50101 aagccagtag atgaactagc agcgcacaga aataaatggc gtaagaaggc aggtgctgaa
    50161 aaatgattga acctggtgta aattatgctg atttatttgc aaaagaagta agaaagaaac
    50221 ctaagaagta tcctaagacc gttcgtttag caatagatcg ctggtatcag tggaaaaaaa
    50281 gaaaagatat ttggtttgat gtagatcgcg cgaatgaaat gatggattgg gtagaatcat
    50341 ttattgttca cacaaaagga gaaatggtgg gaaaaccgtt tcttttagaa ccctgggaaa
    50401 aatttattta ttcttggatt tatggttggg ttaaagaaaa tgaaaaaggc caaattgtac
    50461 gcgttactcg tgaggcttat gtccagattc caaaaaagaa tggtaaaaca ttgattgctg
    50521 tcggttcatt aggatatgca atgtacggag aaggggcgtt atcagttgat tgctatgctt
    50581 gcgcatctga ttttgctcaa gcgcaatatg ctgccaaacc ttttgcagtt acaatcttaa
    50641 acaatccaat cctacttgaa ggaactaaaa tatttagggg gccaaaggga actgtttcta
    50701 gtattactta tgactatatt catgaagata tggcttactc aaataaattt attgttcaaa
    50761 ccaagaatat tgataatatc gagggatcca atccatattt tgtattgaac gatgaattgc
    50821 ataaacagga gaaaatggaa cagtatgaca attttaagtc tgctcagata tctttaccac
    50881 agccattgat gtttaatatc tcaactgctg ggaaaggttc gtcgtctgtt ggaatgcgtg
    50941 tatatcgcga agcaaaagaa gtgttgaaac gtgatgataa tgattcaaac tttgttctaa
    51001 tctatgagcc aaataaaaat tatgattgga cggatagaaa agtctgggaa atgtgcaatc
    51061 ctaactgggg aatatcagtc gatttatctg ctttagaatc ggcatttaaa acagcgcaac
    51121 ggtcagctca ttccaaagct gaatttctaa cgaaacactt ggatgtattt gtgaacggcg
    51181 cggataattt ctttgaacag gatcaagtag agccatgttt agttaccaca caagaacttg
    51241 gtgatttaag tggcgaacca tgttatatcg gtttagattt atcacgtaca cgagatttga
    51301 cctgtgtatc tttaaacttc ccaacatggg atgaagatgg taaagcggtt cttaaagtaa
    51361 agcaacttta ttttattccg aatgaagatt tagagtttcg agaaaaagaa gataatgttc
    51421 cttacagtga tttggctgaa caaggtttcg ttgaattttg cgacggaaaa atgattgatc
    51481 aagatcagat tttgcagtac atagaggact gcatgaattt atatgatatt cagcaagtga
    51541 attatgatcc agcgatgagt gacaaacttg ttgagaaatt agaaaactta ggattggaat
    51601 gcgttgaagt tgctcaatat cctaaagtat tgaatgctcc ttttgacgat gtcgaacggt
    51661 tgttttatga gaaacgaatt caatttgata atccattatt cctttattgc actttgaacg
    51721 ttgtagcaat tactaacatc aatggacaaa aagcgccaag taaacgtcaa tcaaagaaaa
    51781 agattgatgg gttcgtggcg tttttgtgtg gtcataagga aacgatgaat caaatgacag
    51841 atattgattc agatgagtta gatgattacc taagttccat ctatagataa atagaaaggc
    51901 ggtgaaaaaa attgaaatta cgtgatcggt tatcgaatgc agtctattct tttatggaaa
    51961 aacgtggata tatcgaggat atttttggac actacacacg ttatggtcaa agatatgtga
    52021 cggattcatc tatcatggaa tcttctgatg tttatgaatt agttcaagac atatctaatc
    52081 aggtggcatt ggccacacca attgttattg gtcccgacgg caacgaagtc aaagatcatc
    52141 acttactaaa tattttgaag agtcccaatg attatttgac aggattcgaa tttaccaaat
    52201 tggaaacaaa tactttattg atcaatggtg agacattccc attaacggat agggatcagc
    52261 ttcatttggc gtacggtgta acaactaaaa tcaatgaacg acttcaagaa gaatttgaaa
    52321 tgaatggtca aaaaatacct ggtcaaatga ttcgacacat taagaacatc ggaactgatt
    52381 cattaaaagg tgctggaata attgatcttg caagaaacac tctagaaggc gttctgagcg
    52441 ctgaaaaagt tttgacggac aaatatacta aaggcggttt acttgcgttc atgcttaaac
    52501 tagacgccca catcaatcca aataatagcg cccaaacaaa aattgtcaaa gctatattgg
    52561 atcaactgga aggaacgcaa aatgagagtg atcattctgt taagatgatt cctttgggaa
    52621 aaggatattc catcgagaca ttaaaaagtc ctgttgatga cgcggcaatt ttgaattatt
    52681 taggtgttta caaaaaagac ttaggaaaat ttctaggaat caatgttgat acgtatcaat
    52741 cgctgatgaa gacagatatt gaaaaagcga tgatgtatct gcacaacaaa gcaatcaaac
    52801 caatattgaa aaacaagagc gaacattaca ccgctctttt ttttatgcct aattctggct
    52861 atagagtgga atggaaaatt aatattttgg attttgttcc ttactcaaca aaaacaaata
    52921 ttggctacaa catcgttcgt accgggatta caagtccaga taacgtggca gaaatgcttg
    52981 gttttcctaa acagaatact ccagaaacac aagctatcta tatttcaaat gacttatcta
    53041 ggattggcca gaaaaatgca acagatgatt ccttaccaac gaatgatcaa gacttgaaag
    53101 gaggtgatgg aaatgaagaa gaaggaaatt cgcacgattg acatcaccaa tctttcaaca
    53161 cgttccgatg aagaaactca tacgaggacc attagcggct acgctgctgt attcaacagt
    53221 caaacacttt tatgggatga cttaagcgaa gtgattgttc ctggtgcatt ctcaaaggca
    53281 attagtaatt cggatgttcg ttgtttgttc aatcacgatt ggtccaatgt gctaggacgt
    53341 actaaaagcg gaacccttcg ccttgaagaa gatgaacgtg gtttgaaatt cgaagttgat
    53401 ttaccggaca caacggtagc aagggactta gttaaatcta tggaacgagg agacatcaac
    53461 caatgtagtt ttggttttgt gccgactgaa gaaacgtggg actacaattc tgaaccaatg
    53521 cttcgaacaa tccatgaagt ggaattatac gaggtttcta ttgtgccttt gccagcatac
    53581 gaagatacag aagctgcatt aagaagtcgt gatgaattag aaaaaaacgt cgaagaaaga
    53641 aaaaaattaa tcaaaaaaat taatcaagcg ctagaagcgt aggaggaaaa atacatggat
    53701 aaagaattat tgaaaaaaat gaaggcacgt cgtgagcaac gattgactga attacgtgaa
    53761 aaagttgaat caggagaatt acgcgaagca gatttagaag ccgtgaaaga agaaattgac
    53821 agtgttattg atgaattgaa cggaattaaa gacgaattag gcgcagattc tggaactgat
    53881 gaaacagacg acaatacaga tgatcaatcg aatagtacgg attcagacga aagccgttct
    53941 ggtgaagaca acgatcaaga agaagattca gacagtgaag atagttcaga aaatcgttct
    54001 ggaatgatta ctcaacagca acgagatgga ttacttggat caattaagaa cggattggag
    54061 gcacgtgcaa aaatgaccaa gaaacaaaaa gatcaacaac tacgaaaagc atttgctaat
    54121 tttgtagctg gaaatatttc tgaagcagaa gctcgagctt tagggattga agctggcaac
    54181 ggttcagtta ctgtcccaga agtaattgca tctgaagtta ttacttatgc tcaagaagaa
    54241 aatttacttc gtaaatacgg aacagtggtg cgaacatcag gagatgtcaa atatccaatt
    54301 cttgtgaaga aagcagatgc gaatgtaaac aagaaagagc gttcaactga tattgctgaa
    54361 acagctattc agtttgatga aattttgctt aatcctgccg aattcgatgc tttggcaaca
    54421 gtaactaaaa aattattaaa aatgtctggt gttccagttg aagatattgt tgtggaagaa
    54481 ttgaaaaaag cttatgtgcg taaagaaatc aattatatgt tcaatggtga tgacgctgga
    54541 aatgaaaatc ctggtgcatt agccaaaaag gctgtagcat ttaaaaaacc tttagatcta
    54601 actgcttcag gtgctgggca aaaattatat gatgcattaa tcgaatttaa aaatacacca
    54661 gtgacagaag tgatgaaaaa gggacgcttt attattaatc gagctgcttt gactgccatt
    54721 gaaaaaatga aaacagatga tggatttcct ttgttgcggc cattcacaca agcagaaggt
    54781 ggaattggtt accaattagt tggctatcct gtggattgga cagatgcagc agataaaaag
    54841 ggtgaaccag acacgccagt tttatatttt ggcgattttt ctgcatttaa aattcaagaa
    54901 gttattggtg ccttggaaat tcaaaaactt gttgaaaaat tctctggtaa aaatcaaatt
    54961 ggattccaaa tttacaactt gctagatggt caattggttt attctccatt tgaaccggca
    55021 gtatatcgct acgaaattac aaaaccagtt ggtggttaag atggaagagc aaactaaaga
    55081 attgtcttta gaggaaaaat tcaaatcaca tattcatttt gaagagggca tggatgattc
    55141 tttgctctct ttttatttaa atatggcaaa agattatgtc aaaacagcaa ctggtggcca
    55201 acaagaatat cttattttga tggttgccgg cattgcctat gaatatagag tttcagagga
    55261 tgaactcgac aaagctatga atgccatgac gccatttatc gtgcaaggag cgattcaaaa
    55321 tgccgaagag acagaccaat aatctcagat ggaaagccga attgctagac atcaaaacag
    55381 gaacagacgg aaacgatcgt ccaactacaa tttacgaatt taagcgtcta atattctatg
    55441 aagaactcgg tgtgacttct caagaaaaat atttatcaca gcaagccaag acagacgttg
    55501 tcagacgaat taaagtcaga tgggataaat ccatcacaga gaaattaagt gcgctcaaaa
    55561 ttgattctgt aacgtataac attactcgca tttatacgaa tcccgatgca agagaaatgg
    55621 agttgagttt agcttatgtc gattagcttt gatgaattga aaacagcgct gaaatcaaca
    55681 aagttagcag tgttcagaga caaagccaga ttagggacga tgtatccata cattgtgtac
    55741 tcaaatgtga gtaacagcaa aaaaatggca tccggtaaag tctataaaaa attaccttat
    55801 taccaagtct ctttttttac acttggaaca gaacaggatt tagttgttat tgaacaagcg
    55861 ctgcaaaatg ctggcattcc atattcagat tttacaggta tacaaggtga tgagaacgac
    55921 gataccgtaa ccaactatta cacatatgtg aggtgtgtgg aaaatgccaa gtaataagaa
    55981 tggtttttct gaaatatcgg attatttagg aaacctttca aaagttgatc cgaaaaaatt
    56041 atcgttggaa tctttagaag aagcagctaa cttttatcta aagcagttgc ttcctaatat
    56101 tcctaagtcg ctcttaaaaa agaaacatat gcgcgatcaa ataaaagtag tagttgaaaa
    56161 cgatcaagtg aaagttcaat ttgaagagac ggccttttac tggcgctttg ccgaaaatgg
    56221 cacaacaaat caacgtgccc aacattttgc aagtggtacg tatgaacaaa acaaagaaaa
    56281 aattgaagaa attatgacta gaaaaatact tgatttatgg gaagggtgat tttaattggg
    56341 aaaacaagat actttttatt ttgaaggatt ggacgacatt ttaattgcga tgatggcaac
    56401 gccagattct gttggaactg caccgactta tagtgaagtt ttacgattgc caattgccac
    56461 aaaaatcggt gtcaaaggga atggaacagc attagaaaaa tgggcatcca gcaaaatgtt
    56521 tcgtcgtgtg tcgcgagaaa caaaacatga acttgcgttg gatcacgtgg gtattccgat
    56581 agaagtgatg gatgaaatca aaggattagt tgctaaaagc ggagtaacgt ttagcaaaaa
    56641 taccgcacga gaatttccgt acttcgcctt tggatttatt ggaaatattg aaaatggcgg
    56701 taaaaaagca gtttggtatc ctaatacaca actatccaat gtgattgacg aagagtatgc
    56761 cactgcagag gaagaaacaa aaattgacga tgtaactgca aatctggttt ccatcggact
    56821 gaaatacaat aatgtgatgt atgcaagttt tgattcaaat agagacggag cttctctaga
    56881 attatttaat aaattcattg ctcaacctgt atacgatgaa gaacagtgga aaacattggc
    56941 taaagttgga ggtgcgggtt aatggctcgg ttatctgatt atggaattca cgttgaagac
    57001 ttaaaaaatt ctgctgttgt aattattcaa ggcgcagaat tccctatttc atttactatg
    57061 caaacgatgg aatttatagc agatgtgtat ggtggagatt attcgcaatt tgaatctgat
    57121 atgaacgcca tgctatctaa aaaagaagga caaatttctt ctgctaactt atcgcctagt
    57181 gatctaaaaa tcatgcgtgc cttgatttat gccatgctgc gtactggtgg tttagaggaa
    57241 gatccagaaa ctattttcaa atttttggga atgagtggag aggtgttgtc tgcttatagt
    57301 acctgtatgg aaatttttgc tagccagaca tttcaggtgg aagacctaaa aaaatccaag
    57361 aagccacaag actttcaaaa agcgcaagca aaaagaaaga aaaacaaaaa gaatcggaag
    57421 agataggaac tccttggagt ttttatattt acgttgctct cactctattg aattggagtg
    57481 agagtttctt tttaaagtct acacctaact tgtggctcaa atcttattta cagtggttac
    57541 aacaaaacac cgattttgaa ccacctcaat ctgtaactat ggataaatcg ccttggtggt
    57601 agaaaggagc gctaagataa atggctggta aagaatctga tgtcgttctt aattttaaaa
    57661 cgaatggcga agtcagttat tcgaaaacaa tcaaagaaat caacaaagaa atgaacttag
    57721 ccgctgccga gtacaaaaac caagtgtctg cgatggacaa ggatgcaact caaacagaaa
    57781 aattgcgagc ggctaaacaa aaattagaaa aacaattagg attggctgaa caacgatctc
    57841 aaatgctgag agaggaatat gaaaaatctg tcaaagaaac cggtgaatat tctgctgaat
    57901 cagaaaaact ttataaacaa ttactcaatt ctgaaaccgg agaaaataaa cttcgtacag
    57961 cattagaaca gacgaacgat gcacttaaag aacaaggtga cgtttctgtt gatacagcaa
    58021 aaaaactcca gaaaatcgaa gaaacaggtg agaaagtaaa aggcgttggt gaaaaaatgt
    58081 ctgttggagt aaccgcgcct attgtagcgg caggagcagc aggacttgca gcatttggtg
    58141 aagttgacga ggcacttgat accatcatta caaaaaccgg agcaacaggt gatcaagctg
    58201 atagactttc acagtctttc aaaaacgttg gttcaaatac tcatttacct ttacaaacgg
    58261 ttggagaagc tattggtgag gtaaatacac aatttggatt catggataaa aaactggaag
    58321 attcaaccaa ttatctccta cagtacgctg aaatcaatga tacagatgtt tcgcaatcag
    58381 caatatctgc tcgacaagct attgatgctt atggactaga atatgatgat ttgaattctg
    58441 tccttgatgt aacaacgaaa acatcgcaga atactggtca atctgtagac gacttgatgc
    58501 aaaaagcaat tgatggcgca ccacaaatta aacaattagg gttgagcttt ggtgaagggg
    58561 tcactttact tggacaattt gagcaatctg gtgttgattc aagcgcagct ttaagtagtt
    58621 tatctaaagc aacagtggct tatgcgaaag acggaaaatc tttaagtcaa ggactagaag
    58681 aactacaaga caaggtaaaa aatgcaggtt ctgaaacaga agccatcaat gcagcagcag
    58741 aagtattcgg gactaaaggc ggtcctagaa tggccgatgc aattcgtaga ggtactttga
    58801 acctggaaga tttagcaaaa actgctggag aaagtggggg agctgtagga gacacattcg
    58861 atgctactct cgacccaatt gatcaagccg atcaagctat gaacaatgca aaattagcaa
    58921 tggctgacgt aggtgaagca gtccaagtaa gccttttgcc tttttttgaa aaagcaactt
    58981 ccttattaca ggaattctct aaatggtggg ggtctctaga tcaagataca aagaattgga
    59041 tcataacaat tgctggtata gcggcagcag ttggaccagt actaatcgtt cttggttcac
    59101 ttatgggatc tgttacaaaa atcgttgggg aagttaaaag ttttatagga gtttggcaag
    59161 gacttgctgg tttatttgga atgtcgggag gctggtttgc attagcagtt atagctattg
    59221 gcgcattagt tactggacta atttgggcat ataataacgt taaatggttt catgatggag
    59281 tgaattcttt cttcaaaggt gtttcagatg tagctgttga aatatttaat tttttaagtg
    59341 gttattttgg tggatttttc gatggtgtta tagcaaattt caataatttt tataatgctg
    59401 gaaaacgaat tttcagtggt tttctagatt ttatcactgg agtatttaca gggaattggt
    59461 caaaagcatg gcaaggtcta gttgatattt ttggaggtat tttcgatggt attgttgctg
    59521 tagggaaagc tcctattaat gccatgattg gattgatcaa tggatttatt ggtggattga
    59581 ataatataaa gataccaaaa tgggttcctg gtgtaggtgg aaaatcattt tctatttcaa
    59641 aactgcctta tttagcacaa ggcggtcatc taatcaatgg acaagcgatt gttggtgaag
    59701 ctggacctga gttactgact gccaagaatg gtaaaaccac agttactcca ttatcagacg
    59761 aagaaaaacg caaagggatc agtggaagag tttctggagg tagcattgaa cagcatatac
    59821 acattggcaa agtcgatgca aacaatccat ctgagttaga tcgtatgaat cgtaaatttg
    59881 ctaaagcaaa caaacaggct attcgtgatt tgggaggtgt tcctatttga gtcgacaatt
    59941 tatgaatcca gatgaaccaa attttatttg gaaaaatcgg aatgcagtaa ttgatatgaa
    60001 ttgcatcatt gaggcagagc ttccagatat tatgccgaac aagcgatacg aaacttacac
    60061 gattcagggc cgaagcggag aattgacgga gacgtttgat gactatgaac cttttgattt
    60121 ggaaatagaa gggatcacta ttccacattc gaaactgaga gaagtcaaac gatggctcac
    60181 tggtaaaagc caattaatca cacataatga tccagacaag tatctagagg ctatctgcag
    60241 catggataaa gaagtccctt tcgaaaacga gtggggcttc ttttatacat tcgatgttac
    60301 ttttcgttgt cagccactca aaagaaaaat cggtgaagca cctaaaattt ttcattctag
    60361 tgttatggat atttttgatc atggtgacga aattgcacat ccctatctgg aaatagaatc
    60421 gaacggagga gatataacga tcgctgtcgg tgataaaagc ctaacaatac ttaatacgtt
    60481 agctgcaaca atcgtggttg atactcagct tggaaaagca atacaagaag gtttgaattt
    60541 atttacgcgt ggtgattggc cagtattgca gcctgaatgg aatcaggtaa aaatatccgg
    60601 aaaatttaaa gaagtgcggt tttggaatag gagtgtgtat ctgtgacgca agaatttatt
    60661 tatgcttata aaaaaatgcc agatgatttg agcgtgaacg gagcgtcgtt agttgattgg
    60721 gaagatttgc cagaaatcaa tcgtgtgctg aatggccaat atcgttttta tggtaactat
    60781 tcaagaagcg gacagtatcg ctcttattta aaaaaaggaa attttataaa agcgaaggta
    60841 ccagatggat catggcaata ttttgaaatt tataatgtca aaaagaattt gacctccgtt
    60901 tctgtaacag ctcggcatat tggttttatg gccaataaga actttattgt caagtcgttt
    60961 acggataacg gcaatgggtc tcagatcatg accaatctta aaaacagttt ggcatttgat
    61021 caaaaattta actatctttc gaatgtaggg acaacgcacc aatttacagc aagacaagtc
    61081 gcgccagttg aagcaatcat tggttcaaat aacggtaatc aaaatttgac tggcgtgact
    61141 agcgcggaac ttgatatgga taactatgat ttgaaactgg taaaacagat tggatcagat
    61201 aatggttttc ggattgattt tggcatcaat ttagaagcaa tcgaagaaga aattgatgaa
    61261 gaatcaattg tcaatagttt gtatttggtt ggcggtgttc ctgataacga ttatgatgaa
    61321 gacaaagaac ctatcgaata tggttattta gaaattgatg gagtaaccaa cgagaatcga
    61381 cgtattgcca aacgtgaaaa cggagattgt aaaacagttg atgaattgat caaatggggc
    61441 aaaaccttat ttgacaatga tcgtatccat gagccaaaag ctactcatac agtcagtatg
    61501 gtggcgttgg aacacacact cgaatacggt gacatgtatc aagagctagc gtctttacac
    61561 tttggtgatg tcgctcatgt tagggcgaaa gagctagata tcgagataaa agagcgtatg
    61621 gtcgaataca cttatttccc aacgcttggg aaatataagg atattgtttt aggtaatgat
    61681 ttatcactgt acacttcggc agtaaatact caagcacaag aattgaaaaa gaaaatcgac
    61741 aatcgaacag aaacgttagt tcaaaatgtg ttgaatgcga cggcatggat cactggtaat
    61801 tctggtgggc atgtcgtttt tcgtccagaa aaagctccat cagagattct tatcatggat
    61861 actccaaatg tggcgaatgc aaagcgagtg tggcgttgga atttaaatgg cttaggttat
    61921 tcagacaacg gagtaaatgg cccgttcggt attgctatca catcaaaagg agaaattgtt
    61981 gctgacttca tcaaagtagg aacaataaat gcagaagtat tcgaaacttc ctttaatgct
    62041 tatggtgatg tgttaaaact tgtaaaaggc acgcttcaaa tttggaatga aaacaaaaaa
    62101 atcatggagc taaccaaaaa aggtatggaa ttctggaata gaaaagaatc cattggaacg
    62161 atcggaacaa cagattcagc aggaaatcca tttccaaatg ctgtaacacc tacacctctt
    62221 gaggaaaatt ctttggtaat acgtacaaat ggcgatggaa aatacatttt aatttctcca
    62281 actgcagaaa aaggatttgt gttattagga aacggaaaag catattattt cggtgattta
    62341 gatattcagg gaaaacttac tgttagaggt aaagaagtga taccaggaca aaatggcggt
    62401 ccatctggcg gaggagaaac acctggtggg tatcctgatg aattaaaaac agatgcagaa
    62461 aagagagctt ggagaatata cgatattttg tgtaataacg gatttacgaa acaatctgca
    62521 tgtggaatat tggggaacat tcaacaagaa acaggaggaa cttttgatcc tgatactgtt
    62581 caaataggtg gaccagcata cggattagtt cagtgggatg gttcttcata tcctcttgtt
    62641 ggtccagcaa catgggatgg aaaagtttat gttcaaaact tatttaatgc tgcaggtatt
    62701 aaagaaccaa taacgagctt agatgcacaa gttcgattgc ttatttggac atttacaaat
    62761 ggacaatgga tgggcgtagt acaacctacg acggttgatg gatttaaggc ctgtactgat
    62821 ccaagacaag cagcatatgc ttttgaacga aactatgaac gtccggcagc gacacatcct
    62881 gaacgtcaag attatgcagt taactggtat aacaaattta aagatttaaa accaggagga
    62941 gctactggag aagcaggact aaaacattta gagtctttga ttggacaacg aattggcaat
    63001 ggtcagtgtt atggcttgtc tgcagaatat tcaggatatc tgggtggttg tggcatgggt
    63061 gctggaacaa aatatggttt aactcatgtg ataggaaata cttctgcagc atctgatatt
    63121 ggtattgcct atgattggtc tgctgttggt tggaaagtga ttcaaaaccc tagatatgat
    63181 caattagtag ttggtgcaat tattaattgg gcaagaggtg gacaagtggg ttcatggttt
    63241 gcagacggaa cttatggaca tactggcgtt atccgaggct tagctaatgg tcgtatgcaa
    63301 acttatgaac aaaacacaga gttaggtatg atttgtggaa agttagatcg ccagtattat
    63361 agtgcaagtg caatttcttc cattgtcata ccaccaaaat agggaggtga tttaatggca
    63421 aagtggaatg tcatattaag tacaacagaa ccctataatt acgtggggat gattcaagtt
    63481 cgacaaggca ataaaaacac tgaagttatg gaagctacaa ttgtcgaaaa tggtcttcca
    63541 tatgatttat cagaatgtaa ggtatatttt gaatcagttg ttggcggaaa atatccagtc
    63601 caattagaaa caagaattgt ggatgctaaa aaagggaaaa tcaaatatat ttttgataaa
    63661 tattccatgc agtgtttgca tcgacaaaca gccaatttca ttatatttaa aggag
//
LOCUS       DACEKQ010000004        56062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEKQ010000004 DACEKQ010000000
VERSION     DACEKQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.004"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(171..1562)
                     /locus_tag="IW671_000219"
     CDS             complement(171..1562)
                     /locus_tag="IW671_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0897236.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1775..2161)
                     /locus_tag="IW671_000220"
     CDS             complement(1775..2161)
                     /locus_tag="IW671_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0897237.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2217..2840)
                     /locus_tag="IW671_000221"
     CDS             complement(2217..2840)
                     /locus_tag="IW671_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0897238.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNKLFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2904..3572)
                     /locus_tag="IW671_000222"
     CDS             complement(2904..3572)
                     /locus_tag="IW671_000222"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0897239.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3584..4024)
                     /locus_tag="IW671_000223"
     CDS             complement(3584..4024)
                     /locus_tag="IW671_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897240.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4083..4754)
                     /locus_tag="IW671_000224"
     CDS             complement(4083..4754)
                     /locus_tag="IW671_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0897241.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4939..5337)
                     /locus_tag="IW671_000225"
     CDS             complement(4939..5337)
                     /locus_tag="IW671_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0897242.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5334..6107)
                     /locus_tag="IW671_000226"
     CDS             complement(5334..6107)
                     /locus_tag="IW671_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0897243.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6104..6787)
                     /locus_tag="IW671_000227"
     CDS             complement(6104..6787)
                     /locus_tag="IW671_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0897244.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6954..7841)
                     /locus_tag="IW671_000228"
     CDS             complement(6954..7841)
                     /locus_tag="IW671_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897245.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7838..8707)
                     /locus_tag="IW671_000229"
     CDS             complement(7838..8707)
                     /locus_tag="IW671_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897246.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8844..10130)
                     /locus_tag="IW671_000230"
     CDS             complement(8844..10130)
                     /locus_tag="IW671_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897247.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10403..10981
                     /locus_tag="IW671_000231"
     CDS             10403..10981
                     /locus_tag="IW671_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0897248.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10981..11910
                     /locus_tag="IW671_000232"
     CDS             10981..11910
                     /locus_tag="IW671_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897249.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11907..13541
                     /locus_tag="IW671_000233"
     CDS             11907..13541
                     /locus_tag="IW671_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0897250.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSINQLLSNAQHHYEQTIQD
                     EKKKRSLEIQTMQAQIQPHFIYNTLTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKK
                     EEVIPVKEEIKSVQSYIDLLSMRYGERISAKIMIPDELQSLSMPNMILQPIIENAYLH
                     AFHNKTQGFITVYGKTKNQLLLFEVIDNGDGFDTENTKQKNDHFSGIGISHVDERIRL
                     MYGDAYGLTIQSVIGTGTTVTICLPLLPKNQSEYQK"
     gene            complement(13604..14440)
                     /gene="pheA"
                     /locus_tag="IW671_000234"
     CDS             complement(13604..14440)
                     /gene="pheA"
                     /locus_tag="IW671_000234"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0897251.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14437..14946)
                     /locus_tag="IW671_000235"
     CDS             complement(14437..14946)
                     /locus_tag="IW671_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0897252.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(14939..16234)
                     /gene="aroA"
                     /locus_tag="IW671_000236"
     CDS             complement(14939..16234)
                     /gene="aroA"
                     /locus_tag="IW671_000236"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0897253.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16247..17338)
                     /locus_tag="IW671_000237"
     CDS             complement(16247..17338)
                     /locus_tag="IW671_000237"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0897254.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17355..18521)
                     /gene="aroC"
                     /locus_tag="IW671_000238"
     CDS             complement(17355..18521)
                     /gene="aroC"
                     /locus_tag="IW671_000238"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0897255.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18523..19587)
                     /gene="aroB"
                     /locus_tag="IW671_000239"
     CDS             complement(18523..19587)
                     /gene="aroB"
                     /locus_tag="IW671_000239"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0897256.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19620..20642)
                     /gene="aroF"
                     /locus_tag="IW671_000240"
     CDS             complement(19620..20642)
                     /gene="aroF"
                     /locus_tag="IW671_000240"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0897257.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21301..22275)
                     /locus_tag="IW671_000241"
     CDS             complement(21301..22275)
                     /locus_tag="IW671_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0897258.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22256..24139)
                     /locus_tag="IW671_000242"
     CDS             complement(22256..24139)
                     /locus_tag="IW671_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0897259.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24157..26382)
                     /locus_tag="IW671_000243"
     CDS             complement(24157..26382)
                     /locus_tag="IW671_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0897260.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26497..27531
                     /locus_tag="IW671_000244"
     CDS             26497..27531
                     /locus_tag="IW671_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0897261.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27598..28611)
                     /locus_tag="IW671_000245"
     CDS             complement(27598..28611)
                     /locus_tag="IW671_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0897262.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLIWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28689..28877)
                     /locus_tag="IW671_000246"
     CDS             complement(28689..28877)
                     /locus_tag="IW671_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0897263.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29317..29445)
                     /locus_tag="IW671_000247"
     CDS             complement(29317..29445)
                     /locus_tag="IW671_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0897264.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29448..30821)
                     /gene="rlmD"
                     /locus_tag="IW671_000248"
     CDS             complement(29448..30821)
                     /gene="rlmD"
                     /locus_tag="IW671_000248"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0897265.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(30932..31987)
                     /locus_tag="IW671_000249"
     CDS             complement(30932..31987)
                     /locus_tag="IW671_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0897266.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(31997..33427)
                     /gene="gatB"
                     /locus_tag="IW671_000250"
     CDS             complement(31997..33427)
                     /gene="gatB"
                     /locus_tag="IW671_000250"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0897267.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33424..34896)
                     /gene="gatA"
                     /locus_tag="IW671_000251"
     CDS             complement(33424..34896)
                     /gene="gatA"
                     /locus_tag="IW671_000251"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0897268.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGNPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34896..35201)
                     /gene="gatC"
                     /locus_tag="IW671_000252"
     CDS             complement(34896..35201)
                     /gene="gatC"
                     /locus_tag="IW671_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0897269.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35307..37343)
                     /gene="ligA"
                     /locus_tag="IW671_000253"
     CDS             complement(35307..37343)
                     /gene="ligA"
                     /locus_tag="IW671_000253"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0897270.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37360..39594)
                     /gene="pcrA"
                     /locus_tag="IW671_000254"
     CDS             complement(37360..39594)
                     /gene="pcrA"
                     /locus_tag="IW671_000254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0897271.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39749..41308
                     /locus_tag="IW671_000255"
     CDS             39749..41308
                     /locus_tag="IW671_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0897272.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41419..43350)
                     /locus_tag="IW671_000256"
     CDS             complement(41419..43350)
                     /locus_tag="IW671_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0897273.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43368..44282)
                     /gene="pfkB"
                     /locus_tag="IW671_000257"
     CDS             complement(43368..44282)
                     /gene="pfkB"
                     /locus_tag="IW671_000257"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0897274.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44279..45031)
                     /locus_tag="IW671_000258"
     CDS             complement(44279..45031)
                     /locus_tag="IW671_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0897275.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45190..45399)
                     /locus_tag="IW671_000259"
     CDS             complement(45190..45399)
                     /locus_tag="IW671_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897276.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45410..46738)
                     /locus_tag="IW671_000260"
     CDS             complement(45410..46738)
                     /locus_tag="IW671_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897277.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47103..47690)
                     /locus_tag="IW671_000261"
     CDS             complement(47103..47690)
                     /locus_tag="IW671_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0897278.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47773..49023)
                     /gene="clpX"
                     /locus_tag="IW671_000262"
     CDS             complement(47773..49023)
                     /gene="clpX"
                     /locus_tag="IW671_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0897279.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49270..50559)
                     /locus_tag="IW671_000263"
     CDS             complement(49270..50559)
                     /locus_tag="IW671_000263"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0897280.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50713..51645)
                     /locus_tag="IW671_000264"
     CDS             complement(50713..51645)
                     /locus_tag="IW671_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897281.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51802..52461)
                     /locus_tag="IW671_000265"
     CDS             complement(51802..52461)
                     /locus_tag="IW671_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0897282.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52464..53489)
                     /gene="sppA"
                     /locus_tag="IW671_000266"
     CDS             complement(52464..53489)
                     /gene="sppA"
                     /locus_tag="IW671_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0897283.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53502..53792)
                     /locus_tag="IW671_000267"
     CDS             complement(53502..53792)
                     /locus_tag="IW671_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897284.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54201..56006
                     /gene="glmS"
                     /locus_tag="IW671_000268"
     CDS             54201..56006
                     /gene="glmS"
                     /locus_tag="IW671_000268"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0897285.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT        15651 a        12076 c         9954 g        18381 t
ORIGIN      
        1 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttattcacg gtgtttcaga
       61 agtagcttct cggaaataag ccgaaatttc acaaaaattt gagaaactat ttttaggaaa
      121 cttcttctta tttcttaaag ctgaccactt ctatcacaac ctctttttga ctatgcattc
      181 acatcgatca ctttagaatc atcattgatg acatcgcgat ctaaatcttt gatgcgtgca
      241 cttgtcacga taccagctac catgctaccg ctgacgttta ctgctgtacg tgccatatcg
      301 attaatggtt caacagagat aactaaccca acgattgcta ctggcaagtt cattgcccct
      361 aaaacgatca gtgaagcaaa tgttgcgccc ccaccgactc cagctacacc aaaagaactg
      421 attgttacaa ttgccacaag catcaaaata aattccaaac taaagacatt cactccagct
      481 gctggtgcga cgatcgttgc caacatagct ggataaacgc cagcacagcc gttttgtcca
      541 atagacaaac caaaacttgc tgagaaatta gctgttgcat catctactcc taaagctttt
      601 gtctgagtct cgatattcaa tggcaaagct ccagcacttg aacgtgaagt aaacgcaaag
      661 ctcaataccg gaaaggcttt tttgaaatag ttgattggat tgacttttgc accaatcaaa
      721 atcagtgagt ggacaatcaa aacagcaagc aatgctacgt aagaagcaac gacaaattta
      781 cctaaattga cgatagctgc aaaatcactt gttgccaaaa cgtttgtcat caaagcaaat
      841 actccataag gtgtcaaacg caaaacaagc gtcacgatcc gcataatgat tttatacaga
      901 ctgtcgacta aatctgaaaa gaatgctgct tcttttggcg cttttctttt cacgcctaga
      961 taagcgattc cgacaaatgc cgcgaaaata acgactccaa tcgtacttgt tgcacgacta
     1021 cctgctaaat cagcaaaaac gtttttaggg ataaaggata aaatctgctg tggaatcgat
     1081 aagttttcaa ctgtttcctg acgtgttgcc agttcagaga ttcgtgctgt ttctgcagct
     1141 ccttgtgtga atttagctcc ttggagtccg aacagcattg ttgaaccaat tccgatcaat
     1201 gctgcaatag ctgttgttcc aagtaaagaa gtcaatacag tcgcactaat tttccctagc
     1261 ttttcagaaa ctttcatttt tgtgaatgca ccaatgatcg aaataaacac taaaggcata
     1321 actagcattt gcaagaatga tacatatcct tgacctacaa tcccgatcca ttccattgat
     1381 tgagtaacga ctttgtcgtc tacaccaaag atcaattgaa gcagaccgcc aaagacgata
     1441 ccaactccta atgctgtaaa gacgcgtgtt gaaaattttg tatggcgctt ttgcatacga
     1501 tagaacacat ataataatgc cgcaaaaact agaaccacta aaactgtgat gagtgttgtc
     1561 atttttctcc tcctcatatt attaatgaca agtccatcga aggaagtaaa ggttcccgag
     1621 aatgattctt caaccatcct cctgaaaaaa tttttttcag tgacttttat attttagacg
     1681 atttttatcg aaatgacatt ctttttgctt acaattagta ataaaaaaac atacctgtga
     1741 tagctttaat ctatcgcagg tatgttttta ctgtctattc ttgtttttct gattgaaaat
     1801 tcaattttac cccaggctga tctaaggcga tctctgtcac ctcataagac attcggcgta
     1861 cgatatcgaa taacggtgct actagctcgt ccacttcttc ttgtgtgaca tcattttgac
     1921 cttcaatttt ccgattgagg atatgattga tctgactcac acttccagat aaaacatatg
     1981 tatcaaacac taaacggaac tctaagcgtg ctcctacgat gctatgttct ttcggatagt
     2041 tttcgatcgt ttcttttaaa ggcgagaagc ctactttcaa atctgtttct acttcttggt
     2101 tcggcaatct catatcataa tgaaatgctt ctacaacttc tttttgtctt ctgatttcca
     2161 tataatttta tcactccttt atgaaaatta taacatatct cctttgaaag gggatactac
     2221 tttttaatga tgctctgcaa ggtaaggatt tcattcagat ttcctaccac ataatctgcc
     2281 gggatctcac ccagaaaatg atcgatgtcg taaagacaag tagcgactcc tgcatttttc
     2341 cctgcttcga tatctaaacg acgatcgccg atcatcatcg tgtctgttct ttctaaatga
     2401 aaagtatcga tcaaataatt taaagatgct ggatcaggtt tacgaggaaa gtcctgatcg
     2461 atccctacta cttcctcaat cagatgtgcc aatcgatgtt cttttaacag gccccatgtt
     2521 gattctgtca aacggtgggt caggatgaaa tgacgaccgc cgttgtcttt tagcatttcc
     2581 agtacttgtt tcgtttctgg aaatggacgg gaaacttccg tactttcttt ttcatatcgg
     2641 tgaaataatt tattaaactc tctggcattc aagtttgatt cattgatcaa ataactagta
     2701 gaatactttt tcattttgaa gtagatttct tttttgtcca tactgatacc aaaatctttt
     2761 agtgcttgcc atgcaccatc taccattgcg ggatacgtat caaacaatgt accatcaaag
     2821 tcccatatat aatttttcat gttccttttg ttccattcct ttgcttcttt tttctatctc
     2881 tcacttttag ataaaggtgt tctttaaagt atttttttgt ccagaattgt caaatcatct
     2941 gctaatgtat actggattgg ttgtgcattt gggatatcga tcgtatccat ttgatcttca
     3001 ggaacatctt ctaaatattt tgtcagtgct cggatgctat taccatgacc cacaactagt
     3061 aaatcttgcc gatcttttag ttttggtgct agatgatctt gccataaggg aatgatccga
     3121 ttcactgtca tttccagact ttctccgtat gggatcaaat gcggatccaa gtgatcatat
     3181 ctgcggtcaa atctattttc tgcggttaac ggcggaactt ccgtatatcc tctgcgccat
     3241 gtttttactt gatcttttcc gtatatttct ttcatcttat ctttgttttt acccacaaga
     3301 tccccataat gccgttcatt tagacgccat gtttttatta taggaagcca taactgaccc
     3361 atttcttcta gtgcgacttg tccggtaaga atcgcacgtt tcaaaacaga ggtataaacg
     3421 gcatcgaatt gaagtccggc ttcaagcatt ttttgaccgg caactgctgc ttgttctttt
     3481 cctttttctg ttaaggcgac atctagccat cctgtccagt aattttcaaa atttgcttcg
     3541 ctttcgccat gacgtaataa tactaatttc attcttttct cccttaaaca ttcgtttcga
     3601 attttatacc acatgttttg catgtcacac gtattcgtcc ttttccttta ggtgcacgct
     3661 gcttttgatg acaattcggg cactcaaaaa aagtataact ttttcgatcc attttgactt
     3721 gctttttgtt ttttatctga tgatattttt ctttgactct atcaagatat ttcaaataac
     3781 gttgattctc atttagtctg gggtagattt tttttgatag gaaacgaaat atcatccatc
     3841 cgaaaaagac gatgaacaac aattgtccga ttctataggg caaccaacga cttagcaaac
     3901 taaagacaag cgcagcgatc aatagaccgc gattcaactg atcaaatttc gcatatcttc
     3961 cctgcataag tttgtgatag ttctgctgaa atcgtattag cctttctaac catgtttgca
     4021 tcatattatc tcctccatca tctaatattt attatgacaa aaaaatccaa aaagaaaaag
     4081 ccttaattgg ccttttccat tgcaacttgg tgaaggacat tccgatttct ggtaaagtca
     4141 atcacaatca ctttcttttt atcttctctg ttgactactc gaaatgtgtc cgtcaatgga
     4201 agaaacagct tcttagatgg tagctgttgc atacgtataa aacgatggat catcgtaatc
     4261 aattgaggaa attgaataga cgtgtgaagc agacctggat attttttcaa cagctgtgtc
     4321 gttttgatca gattccgttt tttactgatc acttttaaaa agctttttag aatcttttgc
     4381 tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat ttgtttttgc
     4441 agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa accttctgga
     4501 aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata atcttttact
     4561 ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc tgcttgatag
     4621 atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag caatgtttta
     4681 ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc ttttaaaaca
     4741 actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca tattttgaaa
     4801 agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata agtattcacg
     4861 agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt tgtgtttatt
     4921 tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt catctaaaag
     4981 ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa tgactttgtc
     5041 tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc cttgaccaat
     5101 cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa tatctttttc
     5161 tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac ctcttccatg
     5221 taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat cttcgcccat
     5281 tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat ctttcatttg
     5341 ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc ttttcgttta
     5401 taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat tgcaaccaca
     5461 ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc cacgctcatt
     5521 gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc aaaagccatt
     5581 gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta tcctgggatc
     5641 aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc tactgctgcg
     5701 ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc ctcgctcaat
     5761 tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac taagaacccc
     5821 attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat agcactgcct
     5881 aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag gatcaaccca
     5941 atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg catacgaaaa
     6001 ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa aatgacttcc
     6061 atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg aagatcaccc
     6121 ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt ccaaaactgt
     6181 cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg gccaagataa
     6241 atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc ggcataaatt
     6301 cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc atcaattgat
     6361 aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact ccagtgccga
     6421 cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata tcatatactt
     6481 gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg atagcaaatc
     6541 ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct actagatctc
     6601 cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg atcatcatga
     6661 ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca aaaacaaacg
     6721 ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct ttaaaatttt
     6781 ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag agttttcgag
     6841 cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca cgtcccaaaa
     6901 gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt tttttatttt
     6961 acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac gatggcagtt
     7021 ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt ataggaagca
     7081 cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa agctactaag
     7141 ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc ggcataggta
     7201 tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag accgtctaat
     7261 cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa gaagaagatc
     7321 aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat tcccaaaacc
     7381 gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc aaaaggaacc
     7441 atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg ataaatctca
     7501 aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac caaagcgatc
     7561 atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa attttgaaaa
     7621 ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac gggtgtattc
     7681 gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag gatgcttaat
     7741 gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg ttttttgacg
     7801 ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct cctcctactt
     7861 tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa actgctgccg
     7921 catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga gatagggtgg
     7981 tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa agctgcaatg
     8041 tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt gggatcgtca
     8101 ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca taaatctccg
     8161 aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc caacgccaag
     8221 tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag ccaatcggtg
     8281 tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga gaaaatagac
     8341 ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag atagcggttc
     8401 gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata aaaagtgcta
     8461 gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa aaagcttttt
     8521 taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat gtcatattga
     8581 cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata aaaaccatga
     8641 ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat aaatctttct
     8701 gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga caagaaagac
     8761 aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg aaaaagcgaa
     8821 gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg tttttgtgta
     8881 tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc ttcagctacg
     8941 actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc agatgtccat
     9001 gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc ttgataagct
     9061 tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc aagagtgccc
     9121 atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga atcttctcca
     9181 gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg cgctttttgc
     9241 agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg gttttctgct
     9301 ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc tcctgattgt
     9361 atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc tttcaaaaga
     9421 gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt gactgtctcc
     9481 ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac tcgaccaagg
     9541 tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc tcgcgctact
     9601 tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc tgcttctgcc
     9661 atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac accgtatatt
     9721 ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc ctttacaata
     9781 gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat cctataatct
     9841 tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc ggtgttcagt
     9901 ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc ttcgttctca
     9961 taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca cgcccagaca
    10021 gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt taagaaacct
    10081 gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat ttttatccgc
    10141 tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa atttgaaaac
    10201 ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa aaatacaaat
    10261 caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg gtgaggtttc
    10321 tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa attacaagac
    10381 tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg atgaacagac
    10441 gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc aattaatagg
    10501 gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa tcgatatcgt
    10561 ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc tattaaaaca
    10621 gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg atatggttaa
    10681 agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga gtcctgattt
    10741 ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca acaaaagccc
    10801 aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg ctggacaaac
    10861 tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt atataccgaa
    10921 aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac ccattcattg
    10981 atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga agaatccggc
    11041 tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa gtttcgtcaa
    11101 gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca agatatcaat
    11161 gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca taagggaaag
    11221 aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca gcaagcaagt
    11281 agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga acttgagaat
    11341 ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca tgaggcaatc
    11401 aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa tgaagaactt
    11461 tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt cgaagatttt
    11521 tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa tgcaaaaaaa
    11581 gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta tgagaaagaa
    11641 atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct ctcagccatc
    11701 tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg tatcagtaaa
    11761 gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga gaaagtcggc
    11821 tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat gacaccttct
    11881 caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat gaactctttg
    11941 ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc atttctttgt
    12001 ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg aatgatgtgg
    12061 tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag aatacattag
    12121 ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca gcccaacata
    12181 tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt tcaaaaatcc
    12241 atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact tttgtcggaa
    12301 acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt gctcagagag
    12361 tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg actcttcgcg
    12421 ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg aatcacgttt
    12481 acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt caatctgtag
    12541 taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc atcgcatcaa
    12601 atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca gtaggtgaaa
    12661 caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag gattgtacat
    12721 tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt tttccgatta
    12781 ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg tatttggatc
    12841 gacacacaaa atcgatcaat caattgctaa gtaatgctca gcatcattat gaacagacga
    12901 tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca cagatacagc
    12961 cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag aaaaaaacag
    13021 atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg attgggaaaa
    13081 aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt
    13141 tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat gaactgcagt
    13201 cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat cttcatgctt
    13261 ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt
    13321 tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa caaaaaaatg
    13381 atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta atgtatggcg
    13441 atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta acgatttgtc
    13501 taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc aagcgctgat
    13561 ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta cgcgtctctt
    13621 ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc atcttgattt
    13681 cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg atcacgaaaa
    13741 aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag ttgatttccc
    13801 gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca ggtagtgtca
    13861 agatgacact catttttact ggagctgact gctggctggt catctttcgg tcaccgacga
    13921 tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag atctccaatc
    13981 caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg
    14041 ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca ttaggataat
    14101 gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa
    14161 tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt tcacttttaa
    14221 cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct ccttccaatg
    14281 aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa
    14341 cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat
    14401 aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga tcctttctat
    14461 aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact
    14521 ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc ttgtttttcc
    14581 aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta gacagtccgc
    14641 ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat tttctgcact
    14701 ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct ttcttaaaat
    14761 atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt cggcaatcga
    14821 caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt gtaaacattg
    14881 agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct
    14941 agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat gccggataag
    15001 agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc aaagcagcta
    15061 cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg ccatgcagag
    15121 gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct cccattttct
    15181 gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat tcttctgcgt
    15241 ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg ggcaattcat
    15301 caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga
    15361 cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca
    15421 tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca ttttttaaca
    15481 acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct gatgaaatat
    15541 cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact atgatcgttt
    15601 tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta tggttacggg
    15661 aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca aacagcaaag
    15721 cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat aaagagcggg
    15781 ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag tcagcgccca
    15841 tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa gcatccccga
    15901 acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt atggtcgtac
    15961 cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta ccgcgaccat
    16021 gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg aaggcatgta
    16081 aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa
    16141 ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc
    16201 ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat ttgatccagc
    16261 aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc tgttggtttt
    16321 taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc ttcaaattaa
    16381 tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg ccaggttcat
    16441 ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta tggacaggca
    16501 actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat tggttctcag
    16561 tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat cgttccaata
    16621 atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag gaagcgatcc
    16681 tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac tcttgattga
    16741 attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga cttagcattc
    16801 ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg gttccgcaaa
    16861 atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta aaaatgtaat
    16921 aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga gaaccagcca
    16981 ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg acttgctgct
    17041 tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt tcaaggacat
    17101 caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct gcttgttctg
    17161 cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact gcttgctcac
    17221 gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc tgacgaatac
    17281 aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt tgtgccatct
    17341 tatttgcccc ctttttagta atgtttcaag tagtcacggt actccttcac tgcagctttc
    17401 aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac cacagtggct
    17461 acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga acgttctaca
    17521 cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt gtacaatgta
    17581 ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat tccgccttcg
    17641 aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca taggatctca
    17701 tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa ttctacgcct
    17761 ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt cgtgtcccat
    17821 tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac aattcctccg
    17881 atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg ctcaactgct
    17941 ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg aatcgagata
    18001 tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc tacgatgatg
    18061 tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat ggtcgtctct
    18121 cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt cataccgcct
    18181 accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg gtctttttca
    18241 ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt ttctaccact
    18301 aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat ccgaacacga
    18361 tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg
    18421 tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat aatggctgtt
    18481 aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc ctcctaagta
    18541 atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct tttctagcaa
    18601 gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt ggaataattc
    18661 atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta tcctttctaa
    18721 agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag tgatggctac
    18781 cattccaata gctactcctt ctccatgact gactacccca tatcctgctg tattttcaat
    18841 tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt caaagacatc
    18901 ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa cagcatgttc
    18961 cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg catctgctat
    19021 agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt
    19081 tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga caaggttttt
    19141 cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt gtgcaagtag
    19201 cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca cgaatgctgc
    19261 tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg taaaatcatt
    19321 atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg tctcacccgc
    19381 cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca tgattttttc
    19441 tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag gtctccaaat
    19501 cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa tatcgtattt
    19561 ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa aagctggaat
    19621 taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc tttcatcatg
    19681 cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt tgcaggatca
    19741 ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg agccattggt
    19801 ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg gaaatgagtt
    19861 aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa
    19921 gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc aatgtattct
    19981 gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc aacaggttta
    20041 cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc gccaatctgt
    20101 aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac ttcagtaatc
    20161 actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc ttcttctaaa
    20221 ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag aatttttgcg
    20281 cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc gatagaacaa
    20341 ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac atccacgact
    20401 gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat acgcacagca
    20461 ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt atcacctttt
    20521 agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc ttctttttcg
    20581 atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat aattactatc
    20641 attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc tctacttttt
    20701 caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct cattttcgct
    20761 ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg taggaaattg
    20821 cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca
    20881 ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta gccatcatag
    20941 atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc taaaatagct
    21001 aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc gctccaaact
    21061 gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca tttttatgaa
    21121 acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa ttggttttat
    21181 tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt gttcatgaca
    21241 tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat ctaaagattg
    21301 ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt aaggtgactc
    21361 aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa aaatcgtaag
    21421 aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca gttcttctgc
    21481 tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa
    21541 atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt ctacccattt
    21601 cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg ggagtcctgc
    21661 cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt cccctgatag
    21721 gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac ccaatgcggg
    21781 taattccggt aatccttttt gtccataata atgcatattg acaagcattt taccctctcc
    21841 agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt ccagtgtaaa
    21901 aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta ctggagcaat
    21961 cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt ttggatacaa
    22021 tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt cattatcggt
    22081 cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc cgtttttatt
    22141 gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc ccgatttgac
    22201 acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat gacttttatt
    22261 tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct tgtccatctg
    22321 caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca aagtcaccgc
    22381 catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc tgatctttaa
    22441 aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc atgtaagaat
    22501 ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg aacggttttt
    22561 ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg cagcttttta
    22621 catctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa tggacaagtc
    22681 gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct tccgcataag
    22741 attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg
    22801 tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt acgttgtaag
    22861 aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc gtttccgcca
    22921 tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg gggtaatgag
    22981 atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt tccatatctt
    23041 ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc ccgttgataa
    23101 tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta cgaaaaccga
    23161 aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc aattcaagct
    23221 tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa
    23281 tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca tcaggatcta
    23341 gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa
    23401 gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct tctaaatggt
    23461 tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct
    23521 gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga atattttctt
    23581 cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg gcatacccga
    23641 taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa tgaatcgaga
    23701 tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat
    23761 tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc gtgaaagggg
    23821 gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg atcttgtcat
    23881 aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag gcatcaaaat
    23941 cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg
    24001 atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt gttgcataac
    24061 aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga tcatctaacc
    24121 attttatatt tgctttcata cttttttcct tctctctcat aactttttga aatgaagcag
    24181 ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc
    24241 ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat ctaaccccat
    24301 cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta aaaacacaat
    24361 agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt caaaaggact
    24421 cgccaaacga taataatggc catactggat caccggacga atcgtttgat aggatataat
    24481 ttggtctttt acttgctttt cttcttcagg agacagcttt gtcaaatcca gttcataacc
    24541 aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc tgtcggtctg
    24601 atgatttggt acagctgata catgagctgt gatggacgaa atcggataag caaggcttgt
    24661 tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc atgactgcgg
    24721 catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc cttcccataa
    24781 gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga gcatataacg
    24841 atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat ctgaaagcga
    24901 acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct ttctcatttg
    24961 tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt gtgatctgga
    25021 agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc gatacagttc
    25081 agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc ctttgctatg
    25141 agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact cgaaccagtc
    25201 tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta atacgaacat
    25261 ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac tattgaagtc
    25321 aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt gatgacgacc
    25381 tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt tcaatccctg
    25441 acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat tcaattccca
    25501 cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct gatctttttc
    25561 aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt ctccagtatt
    25621 ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac tgctcgttcc
    25681 tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa tctgtctttc
    25741 attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc
    25801 tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat ttcttatctt
    25861 tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat cgaaataaat
    25921 cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact tgtccatcac
    25981 ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat ggctgtcata
    26041 tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa
    26101 gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca gagaccggtc
    26161 aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat ttttattgtc
    26221 tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc tttcttctat
    26281 ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct tttcatcaaa
    26341 taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac tcccaacaaa
    26401 acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac aattttgtca
    26461 ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca
    26521 cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga tcagacgatc
    26581 catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt ggattatgac
    26641 atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat ctctacgatc
    26701 ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg gatggaagaa
    26761 gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga taatcctaaa
    26821 caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa
    26881 tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa ccctgacatc
    26941 cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac cgtactggaa
    27001 ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa agtcgatatg
    27061 gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc ctatcagcat
    27121 tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg ctggacagaa
    27181 aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct tcctactgcc
    27241 attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat
    27301 atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac aatcgcaaac
    27361 ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca agcagctgtt
    27421 cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt gacttatcct
    27481 gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg aaagagaggt
    27541 tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca caacctctta
    27601 cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt
    27661 gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa acaaggattg
    27721 cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct cctggaatga
    27781 gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat ggtcagccac
    27841 actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca acgaccacgc
    27901 aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg ctgttagctt
    27961 caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg gaagtagctg
    28021 tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat attgcaatgc
    28081 gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta gcaatttgtc
    28141 gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa cttgattagg
    28201 gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt tggcgtctga
    28261 aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga atcctccacc
    28321 atgtacccaa attagaacag gaacaggccc cttgctgttt tttggatagt agatgtcata
    28381 agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca cgtttttttc
    28441 tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca tatggttgat
    28501 caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa acgcgatccc
    28561 aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca taaggctccc
    28621 tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat caagctctcc
    28681 tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa aagaaatttt
    28741 ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc acattgagga
    28801 cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg gtcaatcact
    28861 tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga caactaattt
    28921 atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat cgtattctaa
    28981 ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc
    29041 tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta gtgttgaatc
    29101 atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat tgaaataacg
    29161 aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca caactcaaca
    29221 gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa gaaacaataa
    29281 caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga ttttagggcc
    29341 ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc ggcctccacc
    29401 ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta ttctccttta
    29461 acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac aggctgaact
    29521 ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa agttgccgga
    29581 ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc aataaaatga
    29641 tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat ccctttttct
    29701 tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt ttcgatttta
    29761 ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc catcccatac
    29821 accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga ataagcgtcg
    29881 atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta cagatactct
    29941 gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt ttttcctaat
    30001 agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc taaaatcaca
    30061 tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa ttcttcatga
    30121 atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac taaaacgacc
    30181 atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg tccttcattt
    30241 ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac tactttgatt
    30301 gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag atcatggcta
    30361 ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg gatctgtgct
    30421 ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg aacttctggc
    30481 atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac aagctgttgt
    30541 tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt gttttctaca
    30601 ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc aaacttattg
    30661 cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc gataaataat
    30721 ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac gatttcgact
    30781 gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc ctttaagcct
    30841 atttttcaat cctactgaac tctactataa atcagctaat tacacaacta aaaccggcac
    30901 tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt gtttcgtctg
    30961 ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct tttacgaatt
    31021 ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca tcaggaatct
    31081 gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc atcggtgctt
    31141 ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca tcttctgttt
    31201 caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca tgtcgaccgc
    31261 cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc ttcacaatga
    31321 ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg aatcctccaa
    31381 cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc ccttcatcat
    31441 acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct ttcgcaagat
    31501 aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc aattctgtca
    31561 aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg atgtccattt
    31621 ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct ctaggaattt
    31681 ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt ttaggacggt
    31741 gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca ccagctgcca
    31801 ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct gaatctggtt
    31861 ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc tgtaggatat
    31921 ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt acgcgtgctt
    31981 ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta ttcacgacac
    32041 caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag ccaaccgcac
    32101 gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa atttcattaa
    32161 tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt tcaactactt
    32221 cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg atttttgagc
    32281 tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt gtcaattttg
    32341 tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc atcaaccagt
    32401 tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa tcagacattt
    32461 cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca cggatatagc
    32521 gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga atccattcgt
    32581 catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca ctagaccctt
    32641 ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt gtttcttgtt
    32701 ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa gctaaacctt
    32761 ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg aactcttctt
    32821 gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct tccattttca
    32881 catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa gcgtatgctt
    32941 cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg ccttgtcggt
    33001 tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca tcttcttcta
    33061 aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt tcgatccatc
    33121 cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga ttatctgggt
    33181 aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt aatgccaaag
    33241 ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg cctggataac
    33301 cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct ggcgctggag
    33361 agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg actgtttcaa
    33421 aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt ctgttgcttg
    33481 ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac cgatgatctg
    33541 caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg gaagtccagc
    33601 caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat cgttaatgtt
    33661 ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt cataatctgc
    33721 aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt ttttgaagta
    33781 agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc gttttacttc
    33841 ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt tcacattttc
    33901 agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg cttctgatga
    33961 ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga cttcttccac
    34021 aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt ttacgccagg
    34081 ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt tgatgtcgcc
    34141 tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt ctttttcgtc
    34201 ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg tcattggtcc
    34261 gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac ggccataagt
    34321 aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc ccccagtatc
    34381 actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac caccagaaga
    34441 gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat aagaagtttc
    34501 tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga tcatatctgc
    34561 ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt aaaggatctt
    34621 agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac caattggatt
    34681 accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg cctgttccaa
    34741 tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt ctttgattcg
    34801 ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa gcatatgatg
    34861 cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc cccattatcg
    34921 atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt caataattca
    34981 tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt ttcaaccaca
    35041 ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac catatcgatg
    35101 attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc aagttttgct
    35161 aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct tctttctaaa
    35221 cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa attgtttatt
    35281 gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt agccaatgca
    35341 tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt ggtcagcttg
    35401 ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct accagtcact
    35461 ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg tgttagttta
    35521 ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa ttgggcattt
    35581 cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat caattcatgg
    35641 acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc gatcgtatca
    35701 agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc gaaatgttct
    35761 gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa aatcaaccgc
    35821 tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc tgatttttct
    35881 tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc agcaacatct
    35941 gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc atcgatattc
    36001 atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg gcatttagga
    36061 ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc acaaactgga
    36121 caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa cacgacttgc
    36181 gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt caagcggata
    36241 tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt cgttcctgct
    36301 actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc gactgtccat
    36361 tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc tgctgcccat
    36421 ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt cactttgatg
    36481 acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc ttcaatgtat
    36541 gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg gaatccaatt
    36601 ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc gaaatcagct
    36661 actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc taactgacgc
    36721 aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc tgcttctctg
    36781 tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct tacttcgatg
    36841 ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt ttcagtgatg
    36901 ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc atcttcataa
    36961 cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac tacacgtccc
    37021 actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa aacgtcgttc
    37081 aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac ttttccgcct
    37141 actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc gattgctact
    37201 aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc ttctacgtag
    37261 tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt agcagcttcg
    37321 tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt caattggcgc
    37381 aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta gttctagatc
    37441 cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt gtttgacttt
    37501 atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg gaacaaccgc
    37561 tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg tttttttagg
    37621 cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta caaaacgaga
    37681 aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg ttaaatacaa
    37741 cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct ccagttcgct
    37801 ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa tcaggaatac
    37861 aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt gggaagcatc
    37921 ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga ccgttagttt
    37981 ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt cgaattcttg
    38041 tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta atgtattttg
    38101 gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca attctgtcac
    38161 tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg cacctaattg
    38221 ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt caagcagtgg
    38281 ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc cgatgcctcg
    38341 tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta ccagcacatt
    38401 caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc ctccaaccat
    38461 tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt gtgcgttcgt
    38521 ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct cttcttgcat
    38581 ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt aagtgatctt
    38641 gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat tgttttcaat
    38701 gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt ctagcaaaat
    38761 tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat ctgcaccgcg
    38821 ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa atctacctgc
    38881 caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat ctacatgaat
    38941 ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa ataatcgaat
    39001 cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct ctttttggta
    39061 agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt ttgcaaaatc
    39121 ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac ttcgaggatc
    39181 atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg tcagctgttc
    39241 agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat ctcttcttaa
    39301 aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc cagatgcgag
    39361 aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag taatcgctag
    39421 gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt gtgtcagcac
    39481 tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag tgtgaagaac
    39541 agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg acattaacaa
    39601 catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca ttaaactagc
    39661 tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt tgatttcgcg
    39721 ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca agaaattaga
    39781 taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga caggaatcgt
    39841 cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa gtatctatga
    39901 aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca tccttgcagc
    39961 atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg ggattccaca
    40021 agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt tatggaaaaa
    40081 gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag aaggaccttc
    40141 tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga gtccatcttc
    40201 agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag cattcaatgc
    40261 tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt ctccacttgt
    40321 ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt actttttcgg
    40381 cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat attatggttc
    40441 cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga atgtcttgct
    40501 ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg ggttcgttcc
    40561 tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg gaggcaatca
    40621 aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg gtttgtttgt
    40681 tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg ggattttcct
    40741 tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta tggttgattt
    40801 cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg gttatttcac
    40861 agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg ttggctctct
    40921 taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca atgaccttct
    40981 cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata ctcagaccgc
    41041 ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga gttctttaga
    41101 cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct ctatacgcag
    41161 aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag atattttggt
    41221 tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg ctaaatcttc
    41281 tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta caacaactat
    41341 tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg ggaaaaccca
    41401 gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca ataaaccata
    41461 aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga ccgggtgact
    41521 cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc cgaaagctcc
    41581 gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac gaatcgggtc
    41641 agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca cgatgttcgt
    41701 caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg tagcaacgaa
    41761 aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga cacttccgcc
    41821 agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt tgttgattgg
    41881 accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag cagcattcga
    41941 accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca tagggacatt
    42001 gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa agaggatcgt
    42061 tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa atacgatgac
    42121 ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc ctgtattagc
    42181 taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac ttgaagcaat
    42241 aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt ggttgaacca
    42301 gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca cgccaataaa
    42361 ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga aaggtaacat
    42421 gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac catccgcatt
    42481 tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag taccactaag
    42541 ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac gattgatcag
    42601 ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga ttacaccttc
    42661 tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac cgttcgtttc
    42721 gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt ctgccatgta
    42781 agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc tttctggatg
    42841 agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt ctttttgctc
    42901 ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt ttaattttcc
    42961 tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca aatgtgtgtc
    43021 gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc cgtctaaggc
    43081 ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt taactgcact
    43141 gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt gtgcttcgcg
    43201 ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat caaacatttt
    43261 ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca tgatcatcgc
    43321 ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta atcaattttt
    43381 gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa gtcatcagaa
    43441 aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg atctttcgta
    43501 cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac tgaatttttc
    43561 aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag caaagcaccg
    43621 tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt tttcccatac
    43681 ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa ttcgtgattg
    43741 tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc acctgttgta
    43801 tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg ataaaatcct
    43861 tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg tcctactgtt
    43921 cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc taagccattg
    43981 atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc ttttacggtt
    44041 gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc gccagtgaac
    44101 cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat acgggaaaca
    44161 ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa atcgcctagt
    44221 tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac tgtgtaaatc
    44281 attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat tctaatggac
    44341 aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag gacaactggt
    44401 tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg gctaaacgtt
    44461 taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg gcaccgttca
    44521 ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca ttagatccta
    44581 aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca agctccaaat
    44641 caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt tttccttgca
    44701 gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca tcttcgtcat
    44761 taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt ttgagtgatt
    44821 tttcactcat attctgctca aaattcaata attgctcttt cttagctccg ccatgaactc
    44881 tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt gattcagaag
    44941 cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca agcaagtcta
    45001 ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata caaataatag
    45061 catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa gttataaatc
    45121 agtcaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt tgtctatcat
    45181 caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa cagaagcagg
    45241 ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca tgaccaaaaa
    45301 accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg ataataatga
    45361 tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct catatttttt
    45421 gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc agatgctgct
    45481 cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt ttgaaggtaa
    45541 catcagagag tctatgtttg atttccttta acggtaattc tgacagggat tctgaaggaa
    45601 catagattct taaggataaa agatactctt gatatttttc atctgttacc tctacttcta
    45661 gatattccct aatataataa caataagctt cgattccatt atccaaagca acagtcggat
    45721 cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga aaatacgcct
    45781 gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg atatcataac
    45841 tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt gcattctgat
    45901 actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc ggtttcaata
    45961 aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta aaataggatt
    46021 gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc tggatacata
    46081 tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga aaaatcggtt
    46141 tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa gtgaaacata
    46201 aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact acttccccgt
    46261 tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac aatagccgtt
    46321 gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata taggcgaaca
    46381 ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc ttttctttga
    46441 aaacagagat agacaataca ttttttatca ataattgatt atcttccaag cgaaaactac
    46501 attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat gctagcatta
    46561 ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa gagcggataa
    46621 atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc tgtttttttt
    46681 cttcttcatt taagtctgtg agctggatag tcatcaataa tttatcttcg ccaatcattt
    46741 tctcacctct ttcaaatctt tttataattt aattataacc aatgataaag aaaaactaaa
    46801 actttatgtc ttagaaacca atttggacga ttacatgata gactaagtaa aaagaaacaa
    46861 agaggtgttt acaatggggt tgaaatttga acgtacagat aatttagata aacttttcga
    46921 atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg atcgatttct
    46981 aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag ttaagctcaa
    47041 aaaaagactt accagccaaa cttttatgaa agagtttgac tggtaagtct tttttattct
    47101 ttttatgatt caattgcttg ttcgattgct gcccacgctt cttcttttcc ctgttttgta
    47161 acggaagaaa agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt tttgatttcg
    47221 ctctcatgct tgttccattt accacgaggg atcttatctg ccttagtggc aacaacaatt
    47281 acagggatat cataatactt caaaaattga tacatctgga tatcttcttg agaaggctta
    47341 tgacgcaaat ctactaaaga aacgacagca cgtagctggt ctctagtcgt taaatatgtc
    47401 tcgatcatct ttccccattt tgctcgctca gttttggata cttttgcata cccatatccc
    47461 ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt ctgggtcttt
    47521 cctggcttac cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt gataaaagaa
    47581 gatttcccta catttgatcg tcctgccaga gctatttctg gcaattgtgt atctggatat
    47641 tgttttggtg ccactgcact tatgataatt tcagcttgat ggactttcat ggtctctctc
    47701 cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga cttttacggc
    47761 agcctctttt catcaacccg cttttttctc tgaatcttta tagattactg ttggctcacc
    47821 agacaattca gctgcttctt ttgtgataat cactttttcg atgttttcat ttgaagggat
    47881 atcaaacatc acatccatca tgatttcttc aatgatggaa cgaagtcctc gagcaccggt
    47941 attgcgttcg attgcttttt tcgcaatggc tctcaacgct tcaggttcga attcaagttc
    48001 ggtattatct agagaaagaa gtttttgata ttgtttgact agcgcatttt tcggctcagt
    48061 caagatccgt accagatcat ctgttgtcaa tttctctaaa gctgccataa caggtaatcg
    48121 tccgataaac tcagggatca atccgaattt tagtaaatct tcaggaatga tatgctgcat
    48181 gacactttcg tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg tcttttcgcc
    48241 catacgattc ttcacaatcg tttcgatgcc atcaaatgca ccacctacga tgaacaatat
    48301 gttcgtcgta tcaatctgaa taaattcttg atgtggatgt ttacgtccgc cctgaggtgg
    48361 cacacttgca acagttcctt ctagaatttt cagcaaggct tgttggacac cttcgccgga
    48421 aacgtcacga gtaatagata cattttcgct tttacgcgca attttatcga tctcatcaat
    48481 atagatgatc cctttttctg caagttccac attgtaatca gcagattgaa gcaatttaag
    48541 caaaatgttt tctacatctt cccccacata accagcttct gtcaaacttg tcgcatctgc
    48601 gattgcaaaa ggtacattca atgtttttgc taatgtttgt gctaaaaatg ttttaccaga
    48661 ccctgtaggt ccgatcaagc agatattgct tttttgcagt tcaacatcat ctgtgttttc
    48721 ttgtgcttgc tgattgacac gtttatagtg attgtatacg gcaacagcta acgagcgttt
    48781 tgcgcgatct tgtccaatca cgtagttatt caatacttca agaatttcta gtggtttagg
    48841 gacttcagtg aattcgcgta cagcttcttc ataaaattct tcatcaatga tttctttaca
    48901 taggtcgata cattcattac agatgtagac ccctggacca gctacgatct ttttcacttc
    48961 ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca ttgctacttg gattgtcata
    49021 catggtcttc accccttaac ttgcaaaaag tatgcaagaa actatcaaag actttacagt
    49081 cttttactat cataacatag tttagtaaaa agaaaaagtg tttaacaaag gatcaactga
    49141 cctatacctt tgcatttttt actcttaaca aaaaataagg aactgtgaca aaatcgtgtc
    49201 acagtccctt atttcgaata aacaattttc tagaagtagc ttcttcagaa atgatttatt
    49261 acttgatttc tattctttcg cttctgtcgc tgtacctgtg atcaattcga ttgctttttt
    49321 cattgtcaca tcatgttcca gcatatcttc tgtcaacacg cgtttgattt gttcgattgg
    49381 catgttgtat gtttctgata attcttggat ttctgcattg atttcttctt ctgttgcttg
    49441 aagattttca gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc gcatttctgc
    49501 ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa ccagttaatt gatagtacat
    49561 ttcaggagag ataccttgac gttgcatgtt gttcaagaat tcgtccattg aacgatgtac
    49621 ttcatcatga accatcacgt gtggcaattc aacgatttct gcgttttcaa cagccatacg
    49681 gatcgctgct tcatcttttg catcgtccgc tgctttttct tttgtttcta ctaattcttt
    49741 gcgatatttt tctttcaatt cgtctaatgt tgatacttcg tcatcaacat cttttgcgaa
    49801 ttcgtcatct aattcaggca cttcttttgc ttttacttca tggattgtca ctttgaagac
    49861 tgcttctttt ccagcaagat cttctgcttg ataatcttct gggaaagtta catttacatc
    49921 tagactttca cctgatttat gaccaactaa ttgatcttca aaacctggaa tgaatgagcc
    49981 tgaacctaat tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt cgccatctac
    50041 aaagccttcg aagtcgatta ctactgtatc gccattttca gctgcagcat cttctttgat
    50101 aactaattca gcttgtgctt cttgttcacg tttgatacgt gcatccacgt cagcatctgt
    50161 tacttcacgg tcttgttttt caacaactag atctttatat tgtcctaatt ttacttctgg
    50221 tttcactgtt acttcagcag tgattaccca gtcttgacct ttttccatgc tttcaacatc
    50281 gatttttggt tgagaaacag gatcgatgcc agcttctttg acagcagctt cataagcttc
    50341 tggtaataca gcattcaatg catcttcata taaagcttct tcaccgtaca tacggttgaa
    50401 tacttgacga gaaactttcc ctttacggaa tccaggtacg ttcaagcttt ttttcacttt
    50461 attaaacgca gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa attttaaaac
    50521 accatcattc gtgccttttt tttcaaatgt tgcagtcatt taattccctc cgagtttaga
    50581 aattcatttt atacatcaaa aacgtttcat gcataccttt aaatagtaca ctaacccttt
    50641 caaattgtaa acatattgaa tggatagaat ccttgttttt cataacattt ttatcttttt
    50701 cactgataaa atctaatgga agaagatcat ttgctgcaaa attttttctc ttttttttaa
    50761 aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt tttcctgtac acgcctctac
    50821 agtcagttga aaccagcgct tcgggtcttc gatgtatgta tcatagacag gataaacaat
    50881 tgcagattgc aacgtgaatt cttcttttaa cacggaacta agcagcgcat cttcattccc
    50941 gtaacgatct gaaaatacac ataattgctg gtaacttgcc tgctgctgcg gctttggcag
    51001 atcttttggt atgactttga ctaacttttc ttcaatcgtc aaatagaaaa caggatggca
    51061 ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt gctaaaatat ggacctctgg
    51121 cgatacaata agtttactcg ctgtttggat aaactcatga taaggaagct gttcgatctc
    51181 atcaatagac gcaagctgac gtgcaggttc caacgaagga agttcctccg atttattgtg
    51241 gagtgccttg atcttctgcc tctccatttg tccggataat tcttctgcaa attggatttt
    51301 ttcaatgacc cgctgctcga aagcttcata aaaatctgta cttgctatca attttctagc
    51361 atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa actcctcatt
    51421 ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa
    51481 ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag ctttctttca actcgtaagc
    51541 tcgttgaaat aattcttttg cctgctgata ttgctggttt gctagtgctt gttgtgccac
    51601 tcgcattaaa ttctcatatt tttctggaaa atcaatcggc ttcacgcagt gtcctcctct
    51661 tgctcatatt cagctcatgt tcaatagtag caaaacaaaa aaagcatgac aattaaaaat
    51721 aactgaagca ccctctaaaa gctagatttt agatctgact tttagaaggt gctttcaact
    51781 gttactgctt tttattgctc atcagctgtt gacttcctgg ttgaacgctt tgatcagatt
    51841 gatcgtaaca acacttgtat tagtgaaaaa atcgcccaga tgttgctttt tctgattaaa
    51901 gactgctgga agatacccta acatcagcaa aggaaatttt agaatgaacc ggcaagcacc
    51961 ttctctaact aaaacggttg accagctaag ttcttcttca tcaaagctga tgactcgaat
    52021 accaaagatc attttcccaa tcgtttggcc atgattgaat ttcgtcaata aaataaaata
    52081 cgctagataa ataagtaagg ctgtgaatcc gtagagactc agataagaat ctgaaaaatt
    52141 aagccaacct agatttttgg caataccaag tgttaaacgc gtaatagctc cgatacacaa
    52201 aagatcgacc aagaaggcaa acattcggat aaaaaaacct gcatagaaat acttaggaaa
    52261 atcatttgct tttggcggtt catttcttgt gtccgtatac tttttccatt tacgttgttt
    52321 ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta ccttttggga
    52381 aaagttggag tcaggtccta cagcaggctg ctgttgctta tctttttctt gttccttatt
    52441 ttcatttgaa agctctgaca tgcttactca cctccgtaat aatacattgc ttttggtgat
    52501 tccgctgtcc ctagattctc aagaacggat aagatctgac tagtctcaga aggcttcaat
    52561 ccctggaatt ctgctagttt acttcctaac catgtactag caaacccaga tggcgtggaa
    52621 gaatattcta ctagttccgc atcttcaagc tgttgttcct ttcgtatagc ttttaaagca
    52681 tcttcaggaa atcccaattc atcgactaaa cctacttctt ttgcttggac accatcataa
    52741 atccttccat cagcaatttt tttgacttct tcttcagatt tattccgtcc ctcgctgacg
    52801 attgctacaa aacgactata tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct
    52861 tttgtctctg gtcgcgttcc tgaacccata tctttcaacg ctccgctttt aactgttgta
    52921 tcttccacac ccagtttttc tagaagtcct gaataattca atcctgacat gatcacacca
    52981 attgagccgg tcactgtttc ttctgttgca aagattttat ctgcttgagc agaaatatag
    53041 tagccaccgc tagctgccat gtttttcatg ctgacataca ttgggatatc atgttcttta
    53101 cgaatcgtat ccaatttctt agcgatttcc gcactttcgt agacaccgcc accaggagaa
    53161 tttacttcta aaaagatacc tttgactgtt ggatcttctt caatcgcctt cagttgttcc
    53221 ataaaatttt tatgattgta accttcagaa gcaaataaac ctgaagaccc tcccgcagca
    53281 atcgttccat ctactgataa ttttacgatt ttttgcgtac tgtctccctt ttccaacaca
    53341 tttggagcta gttcttcatc cccatataac caactgttca atccctcaat ttgatcgttt
    53401 tcctgaggct tagtcaaact agctgaaact aacgaaacca caaataatcc tgcagcaatc
    53461 agaactgctg cccaacgtct tttattcatt ctttctcctt tctaatacaa gttttttatg
    53521 acgatcacag tttctgactg ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca
    53581 tacccggaaa acgactcttt tgttaattca tcttctaccg taaactcacg agcttgagca
    53641 acttccaagg catcaggtgc gatttcttgg atgctatccc agccagtatc taaaaatatt
    53701 ttcgtatcat ctgatacacc agctgatgct tcgaatttgg acaattgttt ttttaacatt
    53761 cccagtgtca tgatctcaat ttttgaatcc atttttactc ctcctctatc cgattacttt
    53821 ctattgtacc gaactcctga aaaagatgat agataaaaac ccattatttt ttgacataaa
    53881 aaggacattt gacaaagaat ttgatatata ccaatttatt ttttattctt tttttgttga
    53941 caagaacatt cgcaaaaggt tatgatttta aatgtttcca gataaaaaac aaaaaggagg
    54001 atagttatac cttttaggat agcgccaggt ctgtgtaaac agatgacgag gagagggttt
    54061 atcgaaagat tcggcggatg gccctaggga ctgcactcta cagaaaatcc acaaaaaatg
    54121 tctgcaaaga taaaacaaaa ggattttcct gcagctggaa gcattcatac gaattcatta
    54181 tagaaagtag gatgtttttt atgtgcggaa ttgttggaat gattggatta aaaaatgtaa
    54241 caccaggatt gattgacggt ctagaaaaat tagaataccg tggttatgat tcagctggta
    54301 tctttgtctc agatggcaca actgattatc tggtcaaagc acaaggcaga attcaaaatt
    54361 tgaaaaacaa aataaatatt gatactacag gaaatatagg tattggtcat acacgatggg
    54421 caactcacgg gcaaccttct gaagaaaacg cccatccaca tacttcccaa agtggacgct
    54481 tcgtgctcgt tcacaacggc gtaatcgaaa actttgaaga acttaaaatt gcttatttag
    54541 caaatgacca ctttattggt gagaccgata ctgagattat cgctcatcta attgaaactt
    54601 tcgcgaagga tactacaacg caagaggcgt ttttaaaagc tttgcgggtc atcaaaggtt
    54661 cttacgcttt cgctttgatt gatcggacag cacctgatgt tatttacgtc gctaaaaata
    54721 aaagtccact attaatcgga ctcggcgatg gattcaatgt gattgccagc gatgcaatgg
    54781 ctatgcttgc tcatacgaag gaattcgttg agattgaaga tgaagagatg gttaccgtca
    54841 catcagaaaa agttattatt caaaattttg caggtgacat cgtagaacgt tcttcattcg
    54901 aagcacaagt agatgcttca gatatcgaga aaggtaccta tcctttttat atgctgaaag
    54961 aaatcgacga acagcctatt atcatgcgtc gtatcgctca aaaatacgta acagaagaca
    55021 atcgtgtctc tcttgatcaa aagctggtcg atacattgtc tgatagcgat cgaatctata
    55081 tcgttgcttg cggaactagt taccatgcgg ggttagcagg taaacagact ttggaaaaat
    55141 tgacacagat tcctgttgag gttcatttag ccagtgaatt tggctataac acacctcttc
    55201 tttcaaaaaa accattcttt attttcttga gtcaaagtgg cgaaacagca gacagccgcc
    55261 aagtgctggt aaaaatcaac tgtctcggct acccttcact tacgattaca aatgttgctg
    55321 gttctactct ttcgcgggaa gcatccttta cactgctatt gcatgctgga cccgaaatcg
    55381 cagtagcatc aacgaaagct tatactgcac aaatagctgt actaacttta ttagccaaag
    55441 ctatcggtga taaaaaagaa tttgaaactt ctcttgtatt cgatatcact catgaattga
    55501 gtttagttgc taatgcgatg gaatcggtca tcgcccaaaa agattattta gaagagttgg
    55561 cagaagtata tctcagcgat acaagaaatg ccttttacat tggacgtggt gctgactaca
    55621 acgtttctct tgaagctgct ttgaaattga aagaaatcag ttatatccag gcagaaggtt
    55681 ttgccgctgg cgaattgaaa catggaacga ttgctttgat cgaagaagga acaccagtga
    55741 tcggaatcat cagtgaagaa gtgactggtg cgcatactcg aggaaacctg aaagaagtcg
    55801 aaagccgtgg cgctaaaaca ctagttattg tctcagaggg attagaaaag gaaagtgacc
    55861 agctggtgct accaactgtc cacccttatc taactactct aaccacagtc gtaccgactc
    55921 aattacttgc atattacgca acgctattac ggggattcga cgttgacaaa ccacgtaatt
    55981 tagcgaaatc agtgacagta gaataataaa agaacaagga ccagtgtcag acatctgaca
    56041 ctggtccttg ttcttttatt tt
//
LOCUS       DACEKQ010000005        54254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEKQ010000005 DACEKQ010000000
VERSION     DACEKQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.005"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW671_000269"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW671_000269"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0897286.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW671_000270"
     CDS             complement(937..1518)
                     /locus_tag="IW671_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0897287.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW671_000271"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW671_000271"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0897288.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW671_000272"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW671_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0897289.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW671_000273"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW671_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0897290.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW671_000274"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW671_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0897291.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW671_000275"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW671_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0897292.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW671_000276"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW671_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0897293.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW671_000277"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW671_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0897294.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW671_000278"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW671_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0897295.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW671_000279"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW671_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0897296.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW671_000280"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW671_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0897297.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW671_000281"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW671_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0897298.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW671_000282"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW671_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0897299.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW671_000283"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW671_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0897300.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW671_000284"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW671_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0897301.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW671_000285"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW671_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0897302.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW671_000286"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW671_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0897303.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW671_000287"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW671_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0897304.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW671_000288"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW671_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0897305.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW671_000289"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW671_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0897306.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW671_000290"
     CDS             13972..14157
                     /locus_tag="IW671_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0897307.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW671_000291"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW671_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0897308.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW671_000292"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW671_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0897309.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW671_000293"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW671_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0897310.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW671_000294"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW671_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0897311.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW671_000295"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW671_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0897312.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW671_000296"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW671_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0897313.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW671_000297"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW671_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0897314.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW671_000298"
     CDS             18232..18879
                     /locus_tag="IW671_000298"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0897315.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW671_000299"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW671_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0897316.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW671_000300"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW671_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0897317.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW671_000301"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW671_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0897318.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW671_000302"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW671_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0897319.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW671_000303"
     CDS             20317..21255
                     /locus_tag="IW671_000303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0897320.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW671_000304"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW671_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0897321.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW671_000305"
     CDS             21935..22774
                     /locus_tag="IW671_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0897322.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW671_000306"
     CDS             22750..23619
                     /locus_tag="IW671_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0897323.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW671_000307"
     CDS             23609..24406
                     /locus_tag="IW671_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0897324.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW671_000308"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW671_000308"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0897325.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IW671_000309"
     CDS             complement(25688..28357)
                     /locus_tag="IW671_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0897326.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IW671_000310"
     CDS             complement(28595..28783)
                     /locus_tag="IW671_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0897327.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IW671_000311"
     CDS             29053..29415
                     /locus_tag="IW671_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0897328.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IW671_000312"
     CDS             29467..31149
                     /locus_tag="IW671_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0897329.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IW671_000313"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IW671_000313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0897330.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IW671_000314"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IW671_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0897331.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IW671_000315"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IW671_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0897332.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IW671_000316"
     CDS             34984..35988
                     /locus_tag="IW671_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897333.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IW671_000317"
     CDS             35989..36933
                     /locus_tag="IW671_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897334.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IW671_000318"
     CDS             36933..37895
                     /locus_tag="IW671_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897335.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IW671_000319"
     CDS             37922..38836
                     /locus_tag="IW671_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897336.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IW671_000320"
     CDS             38862..40643
                     /locus_tag="IW671_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897337.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IW671_000321"
     CDS             40991..41677
                     /locus_tag="IW671_000321"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0897338.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IW671_000322"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IW671_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0897339.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IW671_000323"
     CDS             45287..46096
                     /locus_tag="IW671_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0897340.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IW671_000324"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IW671_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0897341.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IW671_000325"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IW671_000325"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0897342.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IW671_000326"
     CDS             complement(49648..50100)
                     /locus_tag="IW671_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0897343.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IW671_000327"
     CDS             50250..51200
                     /locus_tag="IW671_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0897344.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IW671_000328"
     CDS             51193..52152
                     /locus_tag="IW671_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0897345.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IW671_000329"
     CDS             52149..52904
                     /locus_tag="IW671_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0897346.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IW671_000330"
     CDS             52927..53880
                     /locus_tag="IW671_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897347.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17802 a         9833 c        11687 g        14932 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tccgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacg atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg cttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taag
//
LOCUS       DACEKQ010000006        51129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEKQ010000006 DACEKQ010000000
VERSION     DACEKQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.006"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            249..1094
                     /locus_tag="IW671_000331"
     CDS             249..1094
                     /locus_tag="IW671_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0897348.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1375..2595
                     /locus_tag="IW671_000332"
     CDS             1375..2595
                     /locus_tag="IW671_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0897349.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2592..5312
                     /locus_tag="IW671_000333"
     CDS             2592..5312
                     /locus_tag="IW671_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0897350.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5296..6240
                     /locus_tag="IW671_000334"
     CDS             5296..6240
                     /locus_tag="IW671_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0897351.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6309..7319)
                     /locus_tag="IW671_000335"
     CDS             complement(6309..7319)
                     /locus_tag="IW671_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0897352.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7499..7843)
                     /locus_tag="IW671_000336"
     CDS             complement(7499..7843)
                     /locus_tag="IW671_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0897353.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7836..8270)
                     /locus_tag="IW671_000337"
     CDS             complement(7836..8270)
                     /locus_tag="IW671_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0897354.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8408..9145
                     /locus_tag="IW671_000338"
     CDS             8408..9145
                     /locus_tag="IW671_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897355.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9138..10349
                     /locus_tag="IW671_000339"
     CDS             9138..10349
                     /locus_tag="IW671_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897356.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10556..11335
                     /locus_tag="IW671_000340"
     CDS             10556..11335
                     /locus_tag="IW671_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0897357.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11405..12067
                     /gene="trmB"
                     /locus_tag="IW671_000341"
     CDS             11405..12067
                     /gene="trmB"
                     /locus_tag="IW671_000341"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0897358.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12165..13919)
                     /gene="ade"
                     /locus_tag="IW671_000342"
     CDS             complement(12165..13919)
                     /gene="ade"
                     /locus_tag="IW671_000342"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0897359.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14051..14980
                     /gene="cpaF"
                     /locus_tag="IW671_000343"
     CDS             14051..14980
                     /gene="cpaF"
                     /locus_tag="IW671_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0897360.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14937..15986
                     /locus_tag="IW671_000344"
     CDS             14937..15986
                     /locus_tag="IW671_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0897361.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15983..16261
                     /locus_tag="IW671_000345"
     CDS             15983..16261
                     /locus_tag="IW671_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0897362.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16236..16706
                     /locus_tag="IW671_000346"
     CDS             16236..16706
                     /locus_tag="IW671_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0897363.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16723..17025
                     /locus_tag="IW671_000347"
     CDS             16723..17025
                     /locus_tag="IW671_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897364.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17042..17413
                     /locus_tag="IW671_000348"
     CDS             17042..17413
                     /locus_tag="IW671_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0897365.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17385..17744
                     /locus_tag="IW671_000349"
     CDS             17385..17744
                     /locus_tag="IW671_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897366.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17916..18923
                     /locus_tag="IW671_000350"
     CDS             17916..18923
                     /locus_tag="IW671_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0897367.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18948..20132
                     /locus_tag="IW671_000351"
     CDS             18948..20132
                     /locus_tag="IW671_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0897368.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20569..22152)
                     /locus_tag="IW671_000352"
     CDS             complement(20569..22152)
                     /locus_tag="IW671_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0897369.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEVTVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22730..22873)
                     /locus_tag="IW671_000353"
     CDS             complement(22730..22873)
                     /locus_tag="IW671_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0897370.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23096..23575)
                     /locus_tag="IW671_000354"
     CDS             complement(23096..23575)
                     /locus_tag="IW671_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0897371.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23648..23932)
                     /locus_tag="IW671_000355"
     CDS             complement(23648..23932)
                     /locus_tag="IW671_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897372.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24010..24204)
                     /gene="ssrS"
                     /locus_tag="IW671_000356"
     ncRNA           complement(24010..24204)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW671_000356"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24276..25562)
                     /locus_tag="IW671_000357"
     CDS             complement(24276..25562)
                     /locus_tag="IW671_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0897373.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25700..26371
                     /locus_tag="IW671_000358"
     CDS             25700..26371
                     /locus_tag="IW671_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0897374.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26337..26825
                     /locus_tag="IW671_000359"
     CDS             26337..26825
                     /locus_tag="IW671_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0897375.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26838..27785
                     /gene="prmA"
                     /locus_tag="IW671_000360"
     CDS             26838..27785
                     /gene="prmA"
                     /locus_tag="IW671_000360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0897376.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27786..28550
                     /locus_tag="IW671_000361"
     CDS             27786..28550
                     /locus_tag="IW671_000361"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0897377.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28783..30996
                     /locus_tag="IW671_000362"
     CDS             28783..30996
                     /locus_tag="IW671_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0897378.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31007..31453
                     /locus_tag="IW671_000363"
     CDS             31007..31453
                     /locus_tag="IW671_000363"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0897379.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31624..32883
                     /locus_tag="IW671_000364"
     CDS             31624..32883
                     /locus_tag="IW671_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0897380.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32972..33919)
                     /locus_tag="IW671_000365"
     CDS             complement(32972..33919)
                     /locus_tag="IW671_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0897381.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34313..35605
                     /locus_tag="IW671_000366"
     CDS             34313..35605
                     /locus_tag="IW671_000366"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0897382.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35620..37389
                     /gene="aspS"
                     /locus_tag="IW671_000367"
     CDS             35620..37389
                     /gene="aspS"
                     /locus_tag="IW671_000367"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0897383.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37498..38373
                     /locus_tag="IW671_000368"
     CDS             37498..38373
                     /locus_tag="IW671_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0897384.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38639..40522
                     /locus_tag="IW671_000369"
     CDS             38639..40522
                     /locus_tag="IW671_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0897385.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40597..40881)
                     /locus_tag="IW671_000370"
     CDS             complement(40597..40881)
                     /locus_tag="IW671_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0897386.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40871..41605)
                     /locus_tag="IW671_000371"
     CDS             complement(40871..41605)
                     /locus_tag="IW671_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0897387.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41734..42357
                     /locus_tag="IW671_000372"
     CDS             41734..42357
                     /locus_tag="IW671_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0897388.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42549..43670
                     /locus_tag="IW671_000373"
     CDS             42549..43670
                     /locus_tag="IW671_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0897389.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43677..46805
                     /locus_tag="IW671_000374"
     CDS             43677..46805
                     /locus_tag="IW671_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0897390.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46824..50018
                     /locus_tag="IW671_000375"
     CDS             46824..50018
                     /locus_tag="IW671_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0897391.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50164..50871)
                     /locus_tag="IW671_000376"
     CDS             complement(50164..50871)
                     /locus_tag="IW671_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0897392.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16730 a         8941 c        10625 g        14833 t
ORIGIN      
        1 aatatcgaag ttagtttttc acctaacttc gttttttata gttggttatt ataaaatagt
       61 aatatagctg atttggattc aattcatatt tatttataaa tcaaaaatac agagaatagt
      121 atataaatgt cctttcctat tcttaaaatg atgtttgtac ttacttaaat taggtataat
      181 tagtatatgt tttgtgagaa atttagactt acaataagca gtgaatttat aatcaacgta
      241 taggagacat ggatgaaatg aaactaattg gtatagattt agacggtact cttttgaatt
      301 ctgaccagag cattagtcaa aaaaatgcaa gaacaatgaa acatttaccg gaagactgtt
      361 ttccattcat ttgttctgga agagaagtag aagacattaa taatattctc aaaaaatctg
      421 gtttaaggat accagcagta gggttaaatg gttctgttgg atttgatgga gaaaaaaaga
      481 tatttgagtc ttccttcgat aaaaatgatg taaaaaaaat tgattctgtc ctatcgaaat
      541 ttccaaccaa aatatataca aatctcggaa gttatgaatc tcagaattat aaaaataaaa
      601 tgaaaaaaat ttttcacgag gtagggagtg aattttctat caaagagcta aactatgagc
      661 ttgagtatga aaaatctatt tgttcaatcc cttataataa tattgaggaa attatagaaa
      721 aggatgctat cagcatttac aaatttttta tttttattcc taatcgtcaa ttgaaagcta
      781 atatatttaa ccatttgaaa aagatgctaa atgtgtctgt tactgaatcg gcacctgtaa
      841 atattgaaat cgtacctcaa aatgtatcta aaggtgtggt gtttgatcgt ttggaatcaa
      901 tctacaattt aaagaaacct ttgagaatcg cgatcggaga tagtttaaat gacttatcta
      961 tgtttgaaag tgcagatatt agttttgcaa tgggaaatag tcatcaagaa atcatgaaaa
     1021 aagctgatta tgtcactgtt tcaaatgata aagacggagt atctcaagca tttgaaagaa
     1081 ttcttaccat ataaactaat taaggtagta tagaacatag aattctaaat acggaaaaat
     1141 ggagcataaa ttccagtaca aatagtactt cttcagtgtc aaacgaagcg aatataacat
     1201 agatatttag gattgtgaca aaaatcatgt cacaatcttt ttttcgaata aacttatttt
     1261 ttgaagtcgg ccatttatcg taatttcttt gcataatacg aacaaatttt cggttattga
     1321 tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt tttaatggtg
     1381 aaatttatcc atgcggcaga tttacatatg gatcggtcat ttgaaggact gacaactctt
     1441 gataaaactg tacaggaaaa actattgaag acgaatctga ccgttttatc aaatatcatt
     1501 gaacaggcaa taataaataa cgttgatttt gttttacttg caggagataa ttttcatcaa
     1561 aatcgtcctt cattaaaaat acagaaacat ttttcagaac agatgaaacg gttagaaaag
     1621 aaccatatac ctgtatttat aatattcgga aatcatgatt tctatcaaaa agagcggtat
     1681 tggttctcct ttcctaaaaa tgtccattta tttacatccg aaacggtaga gacaaaaaaa
     1741 ataacgataa aaagtggaga aactgtctct ctcagtggat ttagttatcg tcaaccgtgg
     1801 attcaaaaag acaaagtgat ggaatttcct tctagagaac tcacagatta ccatattggg
     1861 ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact atgctccttt ccaaccctca
     1921 aagatgcaag aaaagggata cgattattgg gctttagggc atatccatgt gccaacggtc
     1981 ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac taaaaaggaa
     2041 caagcttcta catctattct actagttgaa ttaagtaaag gaagttgcca aatacatccg
     2101 attaaagtag cagaggtcta ttggaagaca aaggaaatat cattgaggaa agcaagaacg
     2161 acaaaagagg tcattgttca cattcgtcag cagctttcaa aagtagagga tggatttagt
     2221 ttaattgaga taaaattaaa agattatgac catctcaata cagatgtttt ggaacggata
     2281 caatcaggag agctattaga ctatttgtta gaggaattct cagataggat aaatgatttc
     2341 tttatttggc gtatttctct catagagaat actttttata cgaagacccc acttgctgct
     2401 tctgctaaat tgatggaaca gttatttcaa tactatcaga cacctcaaga tttccaacaa
     2461 attttaaatg aagtatatag tcatcaagaa gcggctagaa tattgagcga actgccggaa
     2521 tatcaagaag aaacacttga aaaagccaag cagctgctta atcaagattt tttatttgag
     2581 gaggatcagg aatgaagatt ttaaggatcg agattgctgc ttttggaaaa tggcgtcaaa
     2641 aaagttttga cttttattca ggcaatcagc tgatctatgg gggaaacgaa gcaggaaaat
     2701 caacgattta tcaatttatt caagcaatat tatttggttt cccttcaaaa ggccgtaaaa
     2761 aaaaggatta tacaccaaaa gacggctcag cctatggcgg caaaatatgg cttaaacatc
     2821 ctgtatatgg tgaatttgct gtcgagcgct acaagcagca aaatcgcgga aaatcgaagg
     2881 tgtggctggg ggatcaagtg ggaagtgatg agttactaga aaaactgatc gcacctttga
     2941 cgaaagagtt attccagcag gtatttactt tccagcaaga gcagttgatg gaactagatc
     3001 atttgagaga aaaagagcta catgatgcgc tgatttcttt aggcatcacg ggcagttctc
     3061 aagtattcca aaagcggcaa gaatattatc agaaagcaca acaattattc aagaaaaaag
     3121 gccaaaaatt actaatcaat caaaaattaa atgaatggaa agagctgcaa gaaaaaattc
     3181 atcaaaaaca agaacaggaa gcttattttg gcaagttgat ccaacaagaa caggaatacg
     3241 agtcgcaaca gcagcagtta ttggcacaat taaaagaggc gaacaaacaa ttttcattag
     3301 ccagacagca agaacttaat ttttctttat atgaagaatg gcaatctctc aaaaaaaaca
     3361 gcactacagg tcttcctgat gagcaacttc ttttagatct ggagtctttc tccaaacggt
     3421 atcagcaaac aaatgagcgt ttggaagaat tagaagcaac actggaaaag catagcggga
     3481 tggatcagca gtcagccaga tattattttt atttagagca ggagcaggaa ctaaaagatc
     3541 tccagcaact tcaatttaca gcggaacaat taaacgaaga atggcaaaga ttacaaattg
     3601 agactagaga aacaaattca gaattgaaga ttttggaaca aaggtggcat tggcagaaaa
     3661 atccgttacc ggaagaattt ttatatgaag aagaatggaa gcatcttctg gctgaagaaa
     3721 gaaggattgc tgatctagaa agccagattt ctgtgcgtct ccaaatagct aaagaacagc
     3781 aagagatgat tgaagcagaa gtgaatgact tggaagagac ctatccagca ttattacaaa
     3841 atcagtcaga gatagcacca gtctcttcat ctaaaccgta tatgctgatg gctggcggag
     3901 caataatcat tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt ttactaattg
     3961 gtgcaggcat gattggctat ttcttctaca aaaggcaacc aaaaattaaa actaaaaatg
     4021 atttctctga gataaaagga atgtggcaag aaaaactagg acaacttgat ctagccttag
     4081 ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa cagattcttc
     4141 agcaagaagt atatcgtttt gcggatcatc ggcacttagg aaagatggat acgctcaaac
     4201 tcatgaaaga atatggacag gaagtcatag attatcagaa attatatggc gtatttgagc
     4261 ggaaacataa aagagaacag gaaatacaag aaaaactacg acaaatcgac caaaaatttg
     4321 agtttgttaa agaatggtta ccgatacaag ataagacaat ttcagaaaaa atggtattgc
     4381 tttcccgttt tatacaagag atggaacaga tgaaatttgc cagaagctat cagcaaaata
     4441 ctttattgaa gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg atacaagaat
     4501 acagcgaaca attgactcaa gcaggcttat cttatccttc ggaagtacct gtctttttac
     4561 aaaaagccaa acaagccgaa cagcagatga aaagatacaa tgaattatcg gaaatgctta
     4621 ctccactatt tcctgaagag ctgacaaaag agaaattatc tgtaagactg cgccaactga
     4681 aagaaaagca atcccaactc cagcaagaag aaaaagaagc aagtgaaaat cggcaacgac
     4741 tccaacttca agtagaagag ttgcaaagag acggcacatt ggacgaactt tatcaacaag
     4801 ctagccggca aaaagcaaaa ttacaggaat tactggagga atggggagca cttgaaatcg
     4861 ccgggacatt cttaggtgat ctttcaacag agatgagtga aagacaatta ccccagttgt
     4921 taagagcagc aggtgattat ttaggtattt taacaaaagg aaattatcag aagatattac
     4981 tgaaaaatga tacactgact ttaacagacg gatatacaga atttcccatc tatgagttat
     5041 ctacaggtac gaaagatcag ctgattatgg cgatgagatt tgcttacctc tcattagaag
     5101 ctaaccgttc gatatgcccg atcatcattg atgatggctg gcttcattat gatcatcagc
     5161 gaaagtatca attagctgaa cttttagcta aatttggaga aaagtatcaa ataatttgtt
     5221 tttcttctga taaagaaatg gtaagctatt accatgagat gaaccaaatc gtaaaacaat
     5281 tagaaggagc aaataatgaa aaaaattcgt gatttagtaa tcgatgagca gtttgaacaa
     5341 tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa atttattgct
     5401 tttactttcc aagatacttc aggtactatc gatggtaaat actgggatgc ttcggaggag
     5461 gaaatcaata aatttacagc cggcacagtc gtttttctaa acggtaaaag agaggtttat
     5521 caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa caagggtaga tgagccagga
     5581 gagcctagtt tatacatgga aagagcccct ttgaagaaag aagaaatgga agaagaaatc
     5641 aatcaattgt tgtttgagat caccaatgcg cattggaatc gtattgttcg ctatctactt
     5701 aataaatatc aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca tcatgcattt
     5761 gccagtggat tagcttatca tactttgaca atgcttcact tagggaaatc aatcgccgat
     5821 gaatatcctg agctgaatcg agcattgttg tatgctggta tcattttgca tgatttagga
     5881 aaggtgattg aactttccgg tcctatgtcg acagaatata cgcttgcagg aaatttgatc
     5941 ggtcatctgg tattgattga cgaagaaata actaaagcct gtcttgcgtt gaaaatcagc
     6001 gaagctgacg aagatgttct cgtattacgt catatggttt tagcacacca tggcttatta
     6061 gaatatggct cacctgtacg tccaagaata atggaagcag aaatattaca tcaaatcgac
     6121 aatatcgatg cttcgatgca gatgatgttg acttcgattc gtcaaacaga accaggaaaa
     6181 tacaccgatc ggattttcgg tatggacaac cgaagtttct atgtaccaaa agatttatag
     6241 caaaaattta tttttttcat tcaaaacaaa aaaagcgaga cataagtctt cgcttttttt
     6301 gttttctttt attttgatga atcagttgtt tcttcagttg aagattcttt tgttgaatca
     6361 gttgattttg tgtcagaact ctttgttgaa gctgtagttt cacttgagtc tttcgtactt
     6421 gatgaagttg ttgtgaatgc actcaacaca ttttcaaagg catcatcttt gatttttaca
     6481 tttgcatctt ttaattcttc accgattact ttagttgtga atgcattgtc acttagttta
     6541 gtttcagtag cgatatcttt tagttgatct ttgtatttat ccatgtcatt gcctttattt
     6601 tggtttttca ccattttgac aacgtagtat tctgtcgcat aagaagtagg atttgttgta
     6661 gtaataacat cggagatctc accgtctttc aatttgaaag ctgcttcttt tacttctgca
     6721 ggaatagttg ttgtcgttga gtcgaattta actttaccgc catcttcttt tgtctctgta
     6781 tcagttgatt tgtctttggc taatttagag aaatcatctc catcatccgc tgattttttc
     6841 acatcttttg cttcatcttc acttgataat ttgatgattt gtgcttctac ttctggatgg
     6901 aaagatttcc aagcagtttt caaatcgtca tctgtaatat ctacgtgagc tttcaagcca
     6961 gcttcaaaag ctaaattgtt acgaatatat tctttataag tatctttcgt atatccagca
     7021 gcttctaatt gtgattcaaa agtatcacct aatgatttcg cttgtttgtc gtattctgca
     7081 tccacttgct tatcggttac tttatcgcca tatttgttat tgaatacttt ataaatgatc
     7141 atccgttgta ctaaggattg gttcgatgat tcaagttttg cttcatcata gaaatcagat
     7201 acagtgatcg ttccgccttt catcgttgcg atatctttat tagtatcgcc tgaacaagct
     7261 gctagagtta aaacagccaa cgcactagta gctgccaaaa taattgattt ttttttcatt
     7321 aaaatttgca ctcctgtaat tagttattat ttttatatat taactatacc atatcccatt
     7381 tcctttaaaa acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta
     7441 ggaaatacgc aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt
     7501 atatttgttc aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt
     7561 gtgtgatacg tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt
     7621 ggaaagcagg aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat
     7681 gattcaggct gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca
     7741 gtttctttct tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag
     7801 cagcagctcc aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt
     7861 acgattgttt cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca
     7921 aagtgaatag aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga
     7981 aaaacagatt gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct
     8041 ggaaaagctt tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca
     8101 agttcgggat catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt
     8161 cctttcgttg cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta
     8221 tagcttggga tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc
     8281 ctttctataa agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg
     8341 aaaaaaacaa aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg
     8401 aaaaagaatg agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt
     8461 aaaagatatc agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg
     8521 agcagggaaa agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac
     8581 gatagaaatc aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg
     8641 gtatattcca gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt
     8701 cactgcgatg gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt
     8761 ggagactttt cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat
     8821 gaaacagaaa gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga
     8881 cgaaccattt ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga
     8941 aatgaaaaaa gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa
     9001 atattgtgat cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga
     9061 gatgttgaga aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt
     9121 aacaaaggag caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga
     9181 aaatgatgaa atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct
     9241 tgattggcgg tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg
     9301 caattggagg gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg
     9361 ctacttttgc agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa
     9421 cctatttgga acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg
     9481 catgcggatt ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt
     9541 tcgtctatct gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag
     9601 aattgtttcg actagatcaa caagttattc gttattggaa attaggctgg tttttttctt
     9661 ctctgatctt tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat
     9721 tagtacaggc aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt
     9781 gggagaaaat ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt
     9841 tattcacaga tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta
     9901 tattgactca tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat
     9961 tggcaagagg ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat
    10021 tgattgccgg agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt
    10081 tgatcggttt ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc
    10141 tttatccaat ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat
    10201 taattatcgc gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc
    10261 gatgggttcc gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac
    10321 caactcgttt gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat
    10381 gaaaaataga gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg
    10441 ttttttgaat ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg
    10501 cgactagaat aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga
    10561 gtttcagtta gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata
    10621 cataggcatg cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc
    10681 tgctctttca agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg
    10741 cgatactttg acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg
    10801 tcaaagaaac gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac
    10861 catgctgacc cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga
    10921 aaaagaatta ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct
    10981 tcgtgagcag atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa
    11041 ccatcgtaat tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat
    11101 gtttgcagac cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag
    11161 ctggaatcag tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt
    11221 tcattggtat gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga
    11281 tcatcgacgg atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt
    11341 ggcccaaact ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata
    11401 aataatgcgt gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt
    11461 tgtaattagt gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca
    11521 tccaatccac attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc
    11581 ccatcctgaa atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact
    11641 agacaagttg atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc
    11701 gttgacagag tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc
    11761 atggccaaaa aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga
    11821 tgaacagatt cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt
    11881 tgagtattct ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga
    11941 tttacatcaa agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc
    12001 aaaaggtcaa cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag
    12061 tctgtgaaac taactttctg ctttcataaa aacattaata tccataaata aatcccagaa
    12121 aatgaacatt acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt
    12181 tcatcggttt ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg
    12241 tcggtgatct tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga
    12301 tcaaaggttt tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca
    12361 tagacgatcg aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct
    12421 atttcctgct gtttgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca
    12481 gcatacagca tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca
    12541 atcgttgtcg ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg
    12601 tttcccgttt gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa
    12661 ggaatgaatc gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta
    12721 ttcgggataa tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt
    12781 ggatttaatg gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca
    12841 gcaaataaat ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact
    12901 tcatgaattg gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg
    12961 gcaatcgcac ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga
    13021 atggcttgta atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat
    13081 ccttctttta ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa
    13141 ttttcaggtg tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga
    13201 accattaaat acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca
    13261 tgatccgtcc gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct
    13321 gcatgcccgt caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct
    13381 gtttcattat ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt
    13441 ttttgtaaaa aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg
    13501 gttacaggta cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct
    13561 tctatcataa actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc
    13621 actacagtag taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt
    13681 gagctttcga tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca
    13741 ttatgtacgc tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct
    13801 acatcttttt ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact
    13861 aggtcagcag gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat
    13921 ggagtgcctc ccaagataaa tttttataag tataacataa attcggtttt tgccaatatt
    13981 tttttgtaaa ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga
    14041 ggggagaata atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa
    14101 acatgcccaa gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta
    14161 tggaaaaaaa cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc
    14221 aagatttaag tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc
    14281 atttacttat tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta
    14341 ccttcaacga gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg
    14401 ttgctttagt tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata
    14461 ccttttcagc ggaccagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga
    14521 agaaaaattg caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct
    14581 gcagctacag atcaatccga aaatagcaca gacttatgat acacttttga gactcgcatt
    14641 acggcatcgt ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc
    14701 tgcggtaaga gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct
    14761 tgaaggtaca ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg
    14821 tttagctggt gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt
    14881 atggagttat caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga
    14941 agaagctcag gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc
    15001 aaaaccagtt tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat
    15061 ccctttattt attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac
    15121 atcagctaaa ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag
    15181 agcaactggt acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg
    15241 aaattgccaa acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg
    15301 catatccgat gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg
    15361 ttttgcctca gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga
    15421 tacaagtctt tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac
    15481 tagtaaggta tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac
    15541 aattattgat tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg
    15601 gaaagctttt tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga
    15661 aacaagaaag tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta
    15721 tgtcattggc tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg
    15781 ttttacaggg agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga
    15841 caagaaaaca gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt
    15901 ttttgggcgt agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg
    15961 gaagcataga ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac
    16021 attgatcgaa atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc
    16081 taatttgtcg cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt
    16141 ggtagacaca caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga
    16201 ggaattgttg aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg
    16261 aaggtaaggt tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac
    16321 ttacaacgat cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag
    16381 tctatcaatt tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg
    16441 tgaatcagat ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg
    16501 ttccgaatcc agataaaata aattgggaag tattagatat tcctgatgag attaccttga
    16561 aacgccatgc ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt
    16621 atcaatttta ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg
    16681 gtcgctatac aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg
    16741 cttatgatcg cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag
    16801 gcagaaaaga aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag
    16861 acgcagcgga tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc
    16921 caaattgtcg ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg
    16981 atcagtattg aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact
    17041 gatgatattg agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt
    17101 aagtgaattt ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga
    17161 agaagaactc aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat
    17221 cgaggataaa cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc
    17281 cggcggacat caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca
    17341 cacaatttct ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa
    17401 aaacgatcag tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc
    17461 ttttatgtac gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta
    17521 atatacgaat caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac
    17581 agtctacgct tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat
    17641 atcagtgggt acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt
    17701 tcacagaaga agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa
    17761 acgccctctt ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat
    17821 aaaaaacagt gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa
    17881 aaattgttat aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag
    17941 ggtttaatca aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt
    18001 tagaagccta tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg
    18061 atggagtgcc gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga
    18121 aactttcttt cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca
    18181 gccagacaga acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat
    18241 ttgtttttct tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata
    18301 ttgcggcagg aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt
    18361 accaaacaga aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta
    18421 caagtgattt gacacaagca aatgttcaat actttcatca agacgggttg caggaattat
    18481 taatagatcc tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg
    18541 aaaaagcaaa agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat
    18601 tgctagagca atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga
    18661 gtggattttt agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt
    18721 atcttcaagg aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa
    18781 gtattttgat cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg
    18841 tgaaacttgc ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa
    18901 actggaaatc atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa
    18961 ttgcaatcaa tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa
    19021 ccgtagttgc gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca
    19081 agtacggcga taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag
    19141 taaaaatgct gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca
    19201 ccggagtagg gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg
    19261 atgagaaagt aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg
    19321 caaacttaat ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg
    19381 tattcgatac atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg
    19441 tgtcttatta tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt
    19501 atgtgtcgga acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca
    19561 cttgccactt aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata
    19621 cttctatggg attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg
    19681 atccagctgt tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca
    19741 atgttttgaa caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg
    19801 atcttgaaaa gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc
    19861 gcatccgaaa atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct
    19921 ttactgctgg tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt
    19981 cttggtttgg ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata
    20041 tctctacaaa agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga
    20101 ttgcccgtga tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg
    20161 aaacgcatgc aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg
    20221 taatttaact tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa
    20281 aagtggtcaa aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata
    20341 ttttattttc attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta
    20401 gatcagcctc gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc
    20461 cgaaacattc attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc
    20521 gtgaaagcac aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag
    20581 ttggccgagc ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg
    20641 ttgctgcaaa aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag
    20701 catttcttca atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat
    20761 caggacgttg attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc
    20821 ggataaatct ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg
    20881 ccgtcgagcg acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc
    20941 gggatcggca aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt
    21001 gatggtgcca ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc
    21061 tgcaccattg tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc
    21121 tttgaatagt acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt
    21181 gccagatttt ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt
    21241 gacgagtgtc agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc
    21301 attgtctcct tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac
    21361 ttctttttgc tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac
    21421 tcgaaaacgg ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt
    21481 ttttgtggtg acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt
    21541 tgtattctgt aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg
    21601 ttcatcgagt aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac
    21661 tcgttcgcct ccgctaagtt ctgttaaccg tgcttcctga cggtgtggaa tccctaagcc
    21721 ttgcaagatc gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg
    21781 ctgcagctca ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa
    21841 tgcttcgtag cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa
    21901 gtagtggttg acggtcactt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa
    21961 gccttttttg cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa
    22021 aaaggttgat ttgccagttc cgttgatacc gactaaggca attttttcac cagcttcaat
    22081 ttgacaggat aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc
    22141 ttgtatgatc atgatctctc tccttgagac cactcaaaaa ggccatggtt atccatggcc
    22201 tgtgttattt aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac
    22261 gtgaattcaa ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca
    22321 tggctaaatg cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc
    22381 atcatagtat tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga
    22441 aagttcaact agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc
    22501 tttttttatc tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac
    22561 cagcgttcag gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt
    22621 tgccatttgc ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat
    22681 cgcgttcagg agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga
    22741 atgatttacc gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta
    22801 gtttggcgat aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat
    22861 cggaaacttc cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac
    22921 accagggcga ctaatcgccc tggtgttcac ttttttttat tttacttgaa ctcattgact
    22981 gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt
    23041 tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag
    23101 ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat
    23161 cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag
    23221 cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct gggatttctc
    23281 tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag
    23341 ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt
    23401 ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg
    23461 ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt
    23521 cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca
    23581 ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga
    23641 gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag
    23701 aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg
    23761 ttaaaatacc cattacgcct aaaacagcat atgcagctcc gtaaaattgt aggaatcctt
    23821 gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat
    23881 agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat
    23941 cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt
    24001 taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt
    24061 caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag
    24121 cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg
    24181 acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa
    24241 tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc
    24301 cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt
    24361 ggctgataat actgagcact cttcagttta tctggtaaat attgctgatt gacccaagcc
    24421 tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg
    24481 taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca
    24541 agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg
    24601 acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca
    24661 gcgttcactg tacgagccgc agcagcggga ttacctaaac ctatatcttc ataaccgata
    24721 accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa
    24781 tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga aataacgtca
    24841 taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg
    24901 atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt
    24961 tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga
    25021 gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca
    25081 tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta
    25141 cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag
    25201 atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg
    25261 tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact
    25321 tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag
    25381 ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg
    25441 gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc
    25501 tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc
    25561 ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc
    25621 ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat
    25681 gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa
    25741 aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga
    25801 aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc
    25861 cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg
    25921 tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga
    25981 agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat
    26041 gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt
    26101 ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg
    26161 cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat
    26221 cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta
    26281 tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga
    26341 aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg
    26401 atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg
    26461 cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa
    26521 ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac
    26581 gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg
    26641 attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt
    26701 ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat cagcaactag
    26761 cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt
    26821 attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag
    26881 tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt
    26941 ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt
    27001 ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac
    27061 cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta
    27121 ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga
    27181 agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa
    27241 actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg
    27301 gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg
    27361 gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat
    27421 atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga
    27481 aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt
    27541 tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct
    27601 tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag
    27661 gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg
    27721 tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg
    27781 aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag
    27841 aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt
    27901 ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg
    27961 ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta
    28021 ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg
    28081 aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg
    28141 caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac
    28201 aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg
    28261 agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg
    28321 aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg
    28381 tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag
    28441 ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat
    28501 taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat
    28561 taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa
    28621 caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt
    28681 tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc
    28741 cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt
    28801 ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga acatgtagca
    28861 tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca
    28921 ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg
    28981 gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact
    29041 ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc
    29101 aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag
    29161 atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta
    29221 cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa
    29281 actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa
    29341 ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg
    29401 atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga
    29461 atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat
    29521 tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta
    29581 gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac
    29641 acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat
    29701 atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa
    29761 atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat
    29821 aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc
    29881 catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta
    29941 aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag
    30001 ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac
    30061 aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac
    30121 ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg
    30181 aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat
    30241 cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga
    30301 tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat
    30361 tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg
    30421 atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa
    30481 gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat
    30541 gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt
    30601 aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt
    30661 catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt
    30721 gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt
    30781 tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg
    30841 aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg
    30901 acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta
    30961 cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt
    31021 tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta
    31081 cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct
    31141 ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag
    31201 tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac
    31261 gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt
    31321 atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt
    31381 cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc
    31441 taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag
    31501 ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc
    31561 agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg
    31621 aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa
    31681 aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca
    31741 aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat
    31801 gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt
    31861 ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt
    31921 atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg
    31981 acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt
    32041 ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag
    32101 gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt
    32161 gatcacataa aaggtgtcga aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt
    32221 gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc
    32281 aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg
    32341 aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt
    32401 ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg
    32461 tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg
    32521 aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta
    32581 tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg
    32641 tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc
    32701 gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta
    32761 cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg
    32821 acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat
    32881 tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt
    32941 catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg
    33001 tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct
    33061 ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta
    33121 aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag
    33181 cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc
    33241 cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa
    33301 gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt
    33361 cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat
    33421 tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg
    33481 cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa
    33541 agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg
    33601 attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag
    33661 cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa
    33721 tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc
    33781 tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat
    33841 tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc
    33901 cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca
    33961 aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga
    34021 ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg
    34081 aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag
    34141 acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga
    34201 aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg
    34261 atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt
    34321 ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt
    34381 cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat
    34441 ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa
    34501 agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac
    34561 agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc
    34621 gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt
    34681 acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt
    34741 ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct
    34801 agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga
    34861 ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa
    34921 tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc
    34981 atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat
    35041 gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta
    35101 ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac
    35161 gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc
    35221 tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt
    35281 agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa
    35341 tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga
    35401 cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt
    35461 agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc
    35521 aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta
    35581 tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat
    35641 tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa
    35701 aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt
    35761 caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc
    35821 agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat
    35881 cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa
    35941 gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg
    36001 aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat
    36061 gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca
    36121 ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc
    36181 catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg
    36241 aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt
    36301 ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa
    36361 gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc
    36421 gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc
    36481 gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa
    36541 gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat
    36601 gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta
    36661 ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt
    36721 ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa
    36781 ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt
    36841 gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc
    36901 ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct
    36961 gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct
    37021 gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaact aggcggaggt
    37081 tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca
    37141 aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct
    37201 ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat
    37261 aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat
    37321 gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata
    37381 gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt
    37441 gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg
    37501 aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc
    37561 gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat
    37621 tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga
    37681 ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg
    37741 tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc
    37801 ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg
    37861 tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg
    37921 ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct
    37981 atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa
    38041 tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat
    38101 actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat
    38161 gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat
    38221 aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt
    38281 agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag
    38341 atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat
    38401 actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa
    38461 agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt
    38521 ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt
    38581 ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat
    38641 ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc
    38701 attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta
    38761 tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca
    38821 ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag
    38881 actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca
    38941 gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa
    39001 ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt
    39061 tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt
    39121 tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac
    39181 atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg
    39241 tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt
    39301 tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa
    39361 tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca
    39421 ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt
    39481 tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac
    39541 ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta
    39601 tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac
    39661 attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt
    39721 tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa
    39781 gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt
    39841 tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga
    39901 aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga
    39961 ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt
    40021 agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact
    40081 ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta
    40141 cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac
    40201 gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa
    40261 agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat
    40321 gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag
    40381 taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac
    40441 acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag
    40501 tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg
    40561 cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac
    40621 ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc
    40681 ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg
    40741 tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg
    40801 tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta
    40861 aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca
    40921 tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc
    40981 cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt
    41041 taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat
    41101 ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt
    41161 ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat
    41221 ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc
    41281 gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat
    41341 ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt
    41401 cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa
    41461 ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta
    41521 aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt
    41581 cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt
    41641 attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca
    41701 tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta
    41761 gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta
    41821 ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga cccattatat
    41881 ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa
    41941 aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat
    42001 ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg
    42061 atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt
    42121 tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg
    42181 gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat
    42241 atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt
    42301 gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat
    42361 gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc
    42421 aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata
    42481 gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag
    42541 gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt
    42601 atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag
    42661 aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg
    42721 atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga
    42781 tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt
    42841 tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt
    42901 ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt
    42961 ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc
    43021 aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa
    43081 gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg
    43141 aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga
    43201 gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc
    43261 aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga
    43321 tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg
    43381 ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga
    43441 tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac
    43501 gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc
    43561 ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc
    43621 aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga
    43681 aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt
    43741 cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga
    43801 cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg
    43861 gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt
    43921 ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac
    43981 gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga
    44041 aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac
    44101 tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc
    44161 gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta
    44221 ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa
    44281 aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag
    44341 aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa
    44401 tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa
    44461 agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa
    44521 cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga
    44581 aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac
    44641 aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac
    44701 tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc
    44761 gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa
    44821 ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata
    44881 aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc
    44941 gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg
    45001 gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca
    45061 ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa
    45121 atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa
    45181 agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg
    45241 gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct
    45301 taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca
    45361 ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa
    45421 aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga
    45481 aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac
    45541 aatctttaga tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa
    45601 gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa
    45661 aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag
    45721 atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag
    45781 actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata
    45841 cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag
    45901 aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg
    45961 aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta
    46021 cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc
    46081 agctaactga gacattccca gatacagaag agatcagcca acagttagaa gaaataagac
    46141 gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc
    46201 aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc
    46261 ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt
    46321 tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc
    46381 tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc
    46441 gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg
    46501 cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg
    46561 ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag
    46621 gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg
    46681 aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat
    46741 tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg
    46801 gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg
    46861 gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg
    46921 tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa
    46981 aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag
    47041 ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc
    47101 caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc
    47161 gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag
    47221 aaacgattga gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta
    47281 tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac
    47341 aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata
    47401 aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga
    47461 cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc
    47521 tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt
    47581 tgcatctgca gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac
    47641 ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt
    47701 ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga
    47761 cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac
    47821 cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc
    47881 tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc
    47941 ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt
    48001 tcgtctatgg cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg
    48061 cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt
    48121 atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat
    48181 atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa
    48241 gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg
    48301 agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg
    48361 atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg
    48421 ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg
    48481 aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc
    48541 aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag
    48601 atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga
    48661 gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg
    48721 gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt
    48781 cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt
    48841 acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg
    48901 ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt
    48961 atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc
    49021 ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat
    49081 tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat
    49141 tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg
    49201 aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac
    49261 aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag
    49321 aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa
    49381 tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc
    49441 gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc
    49501 tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt
    49561 tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct
    49621 cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat
    49681 ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga
    49741 atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag
    49801 aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta
    49861 tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac
    49921 tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca
    49981 tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt
    50041 aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg
    50101 aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa
    50161 aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat
    50221 atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca
    50281 gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc
    50341 ttctggaagc atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg
    50401 gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag
    50461 ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac
    50521 attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc
    50581 aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta
    50641 gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc
    50701 tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg
    50761 atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg
    50821 ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca
    50881 cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg
    50941 tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata
    51001 aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg
    51061 aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat
    51121 tttcgaaag
//
LOCUS       DACEKQ010000007        49888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEKQ010000007 DACEKQ010000000
VERSION     DACEKQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.007"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW671_000377"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW671_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0897393.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IW671_000378"
     CDS             complement(323..892)
                     /locus_tag="IW671_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0897394.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IW671_000379"
     CDS             1078..1980
                     /locus_tag="IW671_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0897395.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW671_000380"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW671_000380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0897396.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW671_000381"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW671_000381"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0897397.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IW671_000382"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IW671_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0897398.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IW671_000383"
     CDS             8548..8733
                     /locus_tag="IW671_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0897399.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IW671_000384"
     CDS             complement(8795..9991)
                     /locus_tag="IW671_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0897400.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPLMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IW671_000385"
     CDS             complement(10161..11597)
                     /locus_tag="IW671_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0897401.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="IW671_000386"
     CDS             11890..12699
                     /locus_tag="IW671_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0897402.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12885..15038)
                     /locus_tag="IW671_000387"
     CDS             complement(12885..15038)
                     /locus_tag="IW671_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0897403.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15332..16180)
                     /locus_tag="IW671_000388"
     CDS             complement(15332..16180)
                     /locus_tag="IW671_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0897404.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16404..18344)
                     /gene="thrS"
                     /locus_tag="IW671_000389"
     CDS             complement(16404..18344)
                     /gene="thrS"
                     /locus_tag="IW671_000389"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0897405.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18681..18860)
                     /locus_tag="IW671_000390"
     CDS             complement(18681..18860)
                     /locus_tag="IW671_000390"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0897406.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18907..20730)
                     /gene="cadA"
                     /locus_tag="IW671_000391"
     CDS             complement(18907..20730)
                     /gene="cadA"
                     /locus_tag="IW671_000391"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0897407.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21008..23287
                     /locus_tag="IW671_000392"
     CDS             21008..23287
                     /locus_tag="IW671_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0897408.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23380..24027)
                     /locus_tag="IW671_000393"
     CDS             complement(23380..24027)
                     /locus_tag="IW671_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897409.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24206..24925)
                     /locus_tag="IW671_000394"
     CDS             complement(24206..24925)
                     /locus_tag="IW671_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0897410.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25056..26207)
                     /locus_tag="IW671_000395"
     CDS             complement(25056..26207)
                     /locus_tag="IW671_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0897411.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26424..27164)
                     /locus_tag="IW671_000396"
     CDS             complement(26424..27164)
                     /locus_tag="IW671_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0897412.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27168..27491)
                     /gene="rsfS"
                     /locus_tag="IW671_000397"
     CDS             complement(27168..27491)
                     /gene="rsfS"
                     /locus_tag="IW671_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0897413.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27548..28141)
                     /locus_tag="IW671_000398"
     CDS             complement(27548..28141)
                     /locus_tag="IW671_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0897414.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28125..28772)
                     /locus_tag="IW671_000399"
     CDS             complement(28125..28772)
                     /locus_tag="IW671_000399"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0897415.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28785..29096)
                     /gene="yhbY"
                     /locus_tag="IW671_000400"
     CDS             complement(28785..29096)
                     /gene="yhbY"
                     /locus_tag="IW671_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0897416.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29112..30221)
                     /gene="yqeH"
                     /locus_tag="IW671_000401"
     CDS             complement(29112..30221)
                     /gene="yqeH"
                     /locus_tag="IW671_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0897417.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30218..30745)
                     /locus_tag="IW671_000402"
     CDS             complement(30218..30745)
                     /locus_tag="IW671_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0897418.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30925..31710)
                     /locus_tag="IW671_000403"
     CDS             complement(30925..31710)
                     /locus_tag="IW671_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0897419.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31703..32572)
                     /locus_tag="IW671_000404"
     CDS             complement(31703..32572)
                     /locus_tag="IW671_000404"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0897420.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32573..33952)
                     /locus_tag="IW671_000405"
     CDS             complement(32573..33952)
                     /locus_tag="IW671_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0897421.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33960..34379)
                     /gene="fabZ"
                     /locus_tag="IW671_000406"
     CDS             complement(33960..34379)
                     /gene="fabZ"
                     /locus_tag="IW671_000406"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0897422.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34404..34880)
                     /gene="accB"
                     /locus_tag="IW671_000407"
     CDS             complement(34404..34880)
                     /gene="accB"
                     /locus_tag="IW671_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0897423.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34883..36118)
                     /gene="fabF"
                     /locus_tag="IW671_000408"
     CDS             complement(34883..36118)
                     /gene="fabF"
                     /locus_tag="IW671_000408"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0897424.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36137..36874)
                     /gene="fabG"
                     /locus_tag="IW671_000409"
     CDS             complement(36137..36874)
                     /gene="fabG"
                     /locus_tag="IW671_000409"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0897425.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36886..37803)
                     /gene="fabD"
                     /locus_tag="IW671_000410"
     CDS             complement(36886..37803)
                     /gene="fabD"
                     /locus_tag="IW671_000410"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0897426.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37910..38134)
                     /locus_tag="IW671_000411"
     CDS             complement(37910..38134)
                     /locus_tag="IW671_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0897427.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38187..39149)
                     /locus_tag="IW671_000412"
     CDS             complement(38187..39149)
                     /locus_tag="IW671_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0897428.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39159..39599)
                     /locus_tag="IW671_000413"
     CDS             complement(39159..39599)
                     /locus_tag="IW671_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0897429.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39905..40870
                     /locus_tag="IW671_000414"
     CDS             39905..40870
                     /locus_tag="IW671_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0897430.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40927..41157)
                     /locus_tag="IW671_000415"
     CDS             complement(40927..41157)
                     /locus_tag="IW671_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0897431.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41243..42130)
                     /locus_tag="IW671_000416"
     CDS             complement(41243..42130)
                     /locus_tag="IW671_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0897432.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42219..43442)
                     /locus_tag="IW671_000417"
     CDS             complement(42219..43442)
                     /locus_tag="IW671_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0897433.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43459..44538)
                     /locus_tag="IW671_000418"
     CDS             complement(43459..44538)
                     /locus_tag="IW671_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0897434.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44669..46396)
                     /gene="ptsP"
                     /locus_tag="IW671_000419"
     CDS             complement(44669..46396)
                     /gene="ptsP"
                     /locus_tag="IW671_000419"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0897435.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46396..46662)
                     /locus_tag="IW671_000420"
     CDS             complement(46396..46662)
                     /locus_tag="IW671_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0897436.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47141..49375
                     /locus_tag="IW671_000421"
     CDS             47141..49375
                     /locus_tag="IW671_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0897437.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49523..49843
                     /locus_tag="IW671_000422"
     CDS             49523..49843
                     /locus_tag="IW671_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0897438.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14254 a        10710 c         8729 g        16195 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattaaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatttt tgtcacaacc ttttttgccg agtaaacggt gttcaaaagc
    12781 cagtttctcg gtatgaagtc aaatttaagc aaaaatgtga gaagctattt tcacaaaatt
    12841 tttcttcata ctcgaaacta agcggctttt tcacgacctc ttttttattt tgccatgaac
    12901 atatcataat acgcgtccat tatttcttta gcaatcgtca agtttatgtg gtcttgttcg
    12961 tctaataagt tcggcaagac aacactgatg gcaatttccg gattgtctgt tggtccgtat
    13021 gccacgatat tactattgac tgtcgcaata tctggatgac cttccgcaac cgtttctgca
    13081 gtccctgttt ttgcggaaag gtccattttc gcactggcta atggtttggc tgttgtgtaa
    13141 ggatcggtac catggacaac ttggtggaat ccttcacgga ggatcgccat attttctgca
    13201 gaaatattga ccgtattttc tactgttgtg ccaatttctt tcttcacttc acccaagttt
    13261 ccattttcgt cattgccata gatcgctttg actaggtgcg gctgtactcg tttcccgcca
    13321 ttagcgactg ttgaagcata ttgagctaat tggattggtg tatatgtgtc aaactgtcca
    13381 aatgccaaat cggtaaagtt acctggagca taccattctt tgccattatc cgcattgaac
    13441 ttcttcatat aatcaacaga acgtgagatc ccaggagatt cattcggcaa atcgatccca
    13501 gtcgttgtac caagaccaaa ctctttgaaa gagtctctca attgctgata agctttagct
    13561 tcttcatcta aactaggtag tcccattcca ggtgtatagt ctagtcctaa catctttaac
    13621 gccactttga tcatatacgt attagaagac aattccgatg ctttgactgc atcaagagca
    13681 acttgtccgc ttcgattaaa gacagagctt ttttctgaag caccttgcag acgaatcggc
    13741 tcatcaatca aaacttgatt tccgctgatc gcgttacttg cccagcctgc tgtcaaagta
    13801 ccagccttga ctacagaccc tggatcaaag gcgctagtaa tcgtgcctaa agcattctcg
    13861 gtgatttctt tggaaccttg ttcatgactg aaaccagtca ttgctagcac ttctcctgtc
    13921 tgaggattca tagccactgc ataagcacca ggcgaatact gtgctttgcc gttgttgatc
    13981 aatgtttgat aattcctttt caaaatttct tctacttttg attggaattc tgcattcata
    14041 gataacatta aattagaacc tttttcccca gagaagattt ctttttgatt cgaaacattt
    14101 ccttcattat caagggaaac ttcatattgg gttttggttc cttgcaggac atcttcatat
    14161 tgtttttcca aataactttg tcctacacgg tcattccgag aataaccttt tgctaggtat
    14221 ttctctgctt catctgctgg aagtccttgc ttttcagtcg tcacactgcc aagaatactt
    14281 ttcagtgaac ttgctgcatt atattctcgt gtccagtctg tgccagtcga aactcctgga
    14341 agttcagaag cgcgttctgc cacaacagct aactcttgat cagtaacacc agagtttttg
    14401 atatagacag tgtttaattc atatgcacta ttcatccgtt taaagatggt agcaattttt
    14461 aattgctcct cgtttaaatt aatctcatca gctgtgactt tattgaccat cgcactatat
    14521 tgttcgctgt tccctaattg cttttctttt gccgaaagtc tttttgtggc ttctttcaaa
    14581 tgtttgggat cagccagcca aaaatctttt ttatctctat ctgtcagatt ttcatctacc
    14641 ggaacatcga tcagcgagtt cagcttcgtt gcgatttcaa gcaaatcttt agccgtaact
    14701 tgtgttcctc tcgtatatgt gatggcctga ttcgctttat tcgtaactaa agcttttcct
    14761 gtggagtcgt agatcattcc gcgaggagta ctttcttgga ccgtaatcgt agaagtagac
    14821 ttcactcgcg cctccatctc ttcgccgttc acgatctgca aataacccaa tctagcaatc
    14881 aacgcaacaa ataacgtaaa tataatgaaa aaaagaaaat ttaagcgaaa aggaacatgg
    14941 gaccggcgaa acgttttgtt cttaggttct tgtgcacctt ttcttatttt ttctaaccaa
    15001 gaagtattta atattttttt cataaaatct ttcatcattt cctatccttc cgcctattta
    15061 cattcatgct tcattgtacc caaaaacttc ttaataaaaa agaaaaaatg ggcggagcaa
    15121 gaaattcttt acctaatttc aagataaaat caactttttg tttcacagca atcacttaaa
    15181 gttacatatc caactatttt tttcattaaa aaataaactt atttttgaaa attatgtttt
    15241 cttaagtcgt gaaacacttt gatggatcat ttatttgtgg aaaactacgt tttgttattc
    15301 taaaaaactt atataaaaaa tagtaaggtt tttatttcga atccataaaa tattcttgga
    15361 ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc atatagaaat
    15421 aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc gcacttcgat
    15481 tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg ctaagcgggt
    15541 tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc agctcggcta
    15601 atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact ttctttttct
    15661 cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg attggcttac
    15721 cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg ccaaccccat
    15781 ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc gtttcttctt
    15841 ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt ttgtttttga
    15901 tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt ccagtctgta
    15961 gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt tttttggaaa
    16021 aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta gctgtcattg
    16081 tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct gctgaagaat
    16141 aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc acccttttaa
    16201 tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata ctggataaaa
    16261 aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca gattctattg
    16321 agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt ctatctacta
    16381 cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac ttctgcttcc
    16441 atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc ataacgacgt
    16501 acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta agggatcttt
    16561 tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc aacttctaca
    16621 cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc atgtgcatca
    16681 acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa agcgcctttg
    16741 tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc gcggtggata
    16801 actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc aaagcgctct
    16861 ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag agctgttttg
    16921 acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct
    16981 agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca cattgcgtca
    17041 tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa acgataatct
    17101 gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt aaattcatct
    17161 ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc tcgtacacgt
    17221 tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa ttcagcgata
    17281 cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat gtgatgaggg
    17341 cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg cgggaacatg
    17401 tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc tcccattatt
    17461 ggtgtatata catgttggta acctagacta acttctttgt ctacgatata acgttcgatc
    17521 gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc tacttctggg
    17581 gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc ttttgcttct
    17641 tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca
    17701 cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac tgataataat
    17761 ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa atcgatgaat
    17821 tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat cagttctact
    17881 ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac acggcgttcg
    17941 atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg caaatcttct
    18001 gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc gatagctggt
    18061 ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat caaatgagct
    18121 gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat ctcaatactt
    18181 gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt accagctaaa
    18241 gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt tgttccagac
    18301 tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt ttcctccttg
    18361 aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag catctcttga
    18421 aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc aaatgcgttc
    18481 ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc
    18541 ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg aagactctca
    18601 aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa taaatagaaa
    18661 aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta tgttcctttt
    18721 ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca
    18781 gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc ttctactaat
    18841 tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt tttcaactcg
    18901 attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg gatctttttt
    18961 tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa cagctccgaa
    19021 taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa taaagatgcc
    19081 aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta ctgcttttgc
    19141 tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag cagctgtaga
    19201 accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg cgtcattcac
    19261 gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta aaatggtcag
    19321 tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt ctgcaatcgt
    19381 ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat caagttttct
    19441 tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc
    19501 tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta tttcttcagc
    19561 tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg cttggattcc
    19621 ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt tcacgttgtt
    19681 cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg catacagaag
    19741 tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca atatcccttt
    19801 ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt
    19861 tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg caactaaagc
    19921 aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct cagcaaatcg
    19981 taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa tcaacgtgaa
    20041 agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt ttgattcttc
    20101 gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag cgatcatttg
    20161 gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt tcttggtttc
    20221 cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt tggtcactcg
    20281 accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa tatttatttc
    20341 atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc ttggggtgtt
    20401 atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct ctagactatc
    20461 tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga cggctaatgt
    20521 tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac gtaacgtctt
    20581 gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga caatcgcaaa
    20641 tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac caataagcaa
    20701 agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat gtgacttaga
    20761 gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct tgactagcac
    20821 tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt ccaaaattca
    20881 tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc agttaaaaag
    20941 tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg gaaggagcaa
    21001 acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga tcgaactaat
    21061 ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg aaatgaatca
    21121 ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag ctttttatga
    21181 atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc atactcttga
    21241 agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg
    21301 tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg cttctttaag
    21361 agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca atctttatta
    21421 tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag atgtcgatct
    21481 gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg tagatacgtc
    21541 ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa gcaaccatat
    21601 gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg acacaacaag
    21661 taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt tgcttcaacg
    21721 tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt
    21781 ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat taggtgaaag
    21841 cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag agttttcttt
    21901 actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc cgatacaagt
    21961 gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag gcatcacaaa
    22021 ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat caaaggaatc
    22081 gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata gactttctct
    22141 attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca
    22201 atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag atccaaatct
    22261 tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg
    22321 taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca atataacgaa
    22381 acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac ataaaatcag
    22441 cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc aaagagaatt
    22501 gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc cagtgacggt
    22561 tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac catcacaagt
    22621 ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag atctaaatca
    22681 aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg tggaccgtga
    22741 agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca ctgattttgc
    22801 taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag gagaccttcc
    22861 tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag ataccattcg
    22921 aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa cacaagaaga
    22981 atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc agctgatttt
    23041 aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt tagcaaaagg
    23101 tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt cttctcagca
    23161 agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga ccctttttac
    23221 ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg agaaaaaagt
    23281 actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt tatttacaat
    23341 aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat ataaatccaa
    23401 atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt cttccatgtt
    23461 ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat tgtcgatcaa
    23521 tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc tgattggata
    23581 gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat cgatcacttt
    23641 attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag gacgatgttt
    23701 caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt caaataaccg
    23761 aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc tataaatcag
    23821 cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg taccgacata
    23881 gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta attttgaaca
    23941 atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga tacgttcaaa
    24001 aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac atacttcata
    24061 tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa aagaaaataa
    24121 atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg aaaaagactg
    24181 aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg aacacatgct
    24241 gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct tgatcttctg
    24301 gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa aaatcagtga
    24361 ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca ccgtaaacga
    24421 tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc attaggtatt
    24481 caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg tcaaacatgt
    24541 aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct gcccgtacgt
    24601 ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt gagccgcttg
    24661 gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct tttgcttttt
    24721 cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga tcaatgacga
    24781 aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc gcgtagattt
    24841 caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt ttctttgcta
    24901 cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga tagtttcata
    24961 acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag ctttttttct
    25021 ttcttccttc attcttctca tttttagtca attatctata ctcggattgg gatttgcccg
    25081 aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct aactaacagt
    25141 tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc ttttttttct
    25201 tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt tttattttgt
    25261 tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa tcgttggatc
    25321 cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa actatcagca
    25381 gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata gatatctgtc
    25441 agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata agattcccaa
    25501 gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag tccagctatt
    25561 ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct ctgatctgtg
    25621 atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc catagggtgg
    25681 acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg cgaagaaaaa
    25741 tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg cggataacta
    25801 tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt ctcttggaca
    25861 aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa acacaaactg
    25921 tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc agctgactga
    25981 accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa agcttcattc
    26041 gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt cccgctcatt
    26101 acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg atagcgatgt
    26161 ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg catcctcctt
    26221 catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat cggaaaaaat
    26281 acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa agctgcactg
    26341 caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta ttcttttctt
    26401 gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca gcgctttgat
    26461 ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga aaagccgctg
    26521 ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg tagttcatct
    26581 tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag aaaatgttcg
    26641 atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt atccccttga
    26701 taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg tacgtcaaat
    26761 aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg ctgtacctct
    26821 tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg atattgtccg
    26881 atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc ttcttcatat
    26941 gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt cactttatat
    27001 ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc caaaatattt
    27061 tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata aagtgtttcg
    27121 tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta atccaaccat
    27181 tcactaagat cgactagcgg agcatctgac catagttttt ccagattata gaaatttcgt
    27241 tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac ccattttccg
    27301 ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt atcaatgatt
    27361 tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat gaaataatct
    27421 gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg ttttgaatct
    27481 gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc ctcctaatat
    27541 gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt ggataaatct
    27601 gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct acagcttcgt
    27661 ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct cgacctggtt
    27721 caatatagtc tgctacataa atgattttgt caaggagact catcttagca gccccagtcg
    27781 tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc cgctctacaa
    27841 acgaagcgcc aacaagacca tgccagattg cattattata attcaataac tccggatcaa
    27901 aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt gcataatcat
    27961 gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg gcaagagcca
    28021 cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt ctcatctgta
    28081 ttttttgcat caggttttct ctagaataag cagtatattc attactatag tccatattca
    28141 tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta gcggatagaa
    28201 cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc aggtacatct
    28261 acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat acccacaaag
    28321 ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc taccatatcc
    28381 ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc cttcatagta
    28441 tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg caaaaaagga
    28501 ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat cgtctgtttc
    28561 tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag acctaaattc
    28621 cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa tgttccgccc
    28681 aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg aaataattgt
    28741 gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat tgcttttact
    28801 tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac aataacacgt
    28861 ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc tgcttcttct
    28921 gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt ttctaaagct
    28981 tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg aaaaatcggc
    29041 tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa actcataatt
    29101 ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt tggcgcccag
    29161 cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa aacgatgtct
    29221 gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc gacttcatca
    29281 ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc tgctttttcc
    29341 agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga agcacgattg
    29401 ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc ttcattcagc
    29461 tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc ttttttacct
    29521 aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa aaaatgcccg
    29581 tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga agttgtgatt
    29641 acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga ttttccgaca
    29701 tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat tttttctaat
    29761 agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac tggacgaatt
    29821 cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt caatgattta
    29881 ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac aaaacggtgt
    29941 agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac gatcaatgca
    30001 tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa tgagacatct
    30061 tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc cccggaagct
    30121 aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt gtttgtttgt
    30181 ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca cctcgccagc
    30241 ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa aaacgattaa
    30301 attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta cgtatccctg
    30361 cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact atctcatcat
    30421 ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt ttcattgctc
    30481 gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt gaattattgt
    30541 ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa gtaagcaact
    30601 cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg gtcaatactg
    30661 ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct tctaccatcc
    30721 atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg aaggagcaca
    30781 taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta cctctatact
    30841 cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag attgatcaca
    30901 gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc gtttttccag
    30961 taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg cttttttcat
    31021 aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag gaataactcg
    31081 atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact cagctgcttc
    31141 tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag aaagcacagc
    31201 ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa gggcaccgcc
    31261 acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag acatttcgag
    31321 cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac cgcaatatgc
    31381 acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt gtttcatcag
    31441 tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc ggatattttc
    31501 ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat ctaatcgagc
    31561 gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc attcctcgaa
    31621 agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag ctcttgctaa
    31681 ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt gtatgcaatt
    31741 caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga tcgacaaaac
    31801 catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc ttgatcgtct
    31861 gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct agaataatat
    31921 cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt aaaacagtta
    31981 gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt gccatctgca
    32041 ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac aagacgactg
    32101 gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg acaatcgttc
    32161 ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga atcccctgaa
    32221 tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc atttttttaa
    32281 ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc gtatcccatt
    32341 cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg attcgaaaag
    32401 cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa aggatatgtt
    32461 tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta gcaggtttgc
    32521 tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc atctatgcgc
    32581 ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa aatgctgtgc
    32641 tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat tctgcgttcg
    32701 tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt aatgcttcaa
    32761 aacgcgtatt tccatggacg attatttttg caatcattga atcataatat ggcgggatcg
    32821 tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct ggaagcagta
    32881 agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct gcattaatcc
    32941 ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc aaaggctcac
    33001 cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt tctgttactg
    33061 gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct tcttcatcca
    33121 tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct cgaacagcag
    33181 cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat tcttccagta
    33241 ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg acatgaccaa
    33301 atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt ttttccaaat
    33361 acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa aaatgctgtg
    33421 ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct gcagctgctt
    33481 tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct actgtagata
    33541 atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat tgacgcgcat
    33601 tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat gtgatattgc
    33661 actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat cctggatgaa
    33721 ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc agatatgaat
    33781 caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta tgaagtgctt
    33841 cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg catgcacgaa
    33901 tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac atatttcacc
    33961 tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac tttagcgatt
    34021 cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag gtataaggta
    34081 tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa ataagccgtc
    34141 tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc cattgcttca
    34201 acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa aaagggctca
    34261 ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc ttccactcga
    34321 tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg tatgttcatt
    34381 gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg ctgtccatat
    34441 tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc ctcactagtt
    34501 atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt ggcaacttga
    34561 tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac gattcccacg
    34621 attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt cattatttct
    34681 ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg caacgtttcg
    34741 tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata caattcaaat
    34801 tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact gactaattct
    34861 ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga accgcattat
    34921 gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt ttgctttcgt
    34981 ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc gtaggtggga
    35041 taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg gctgctccca
    35101 ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa ttttcaccta
    35161 atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt gttccgtgag
    35221 cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca agcttcattg
    35281 ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg tcacagtttg
    35341 caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca tgttctaaag
    35401 attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga ttttcgtcaa
    35461 acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa gctgcaaatc
    35521 cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg acatcagaat
    35581 agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta gcacaagctg
    35641 ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt ccagctgcca
    35701 tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct ttttcatgta
    35761 gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg atgatgacgc
    35821 caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt gcttctaaag
    35881 cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt ttaataaaat
    35941 atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt tctgaagcat
    36001 caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc aaaaagctgt
    36061 ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact cgattcattt
    36121 gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg atgacttgtc
    36181 cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct tctacttgtc
    36241 cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct gctaaaactt
    36301 ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg cctctagttg
    36361 cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta gctgcataat
    36421 ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg atggcccctt
    36481 ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt ccaatcaaat
    36541 tgatatccag acattttttg aaatcttctg catccattcg catcacaagt ttgtcatttg
    36601 tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct gcttctttga
    36661 tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg cattttacac
    36721 cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca ttcaaaatga
    36781 tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct cttgtactac
    36841 ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata gtattgctat
    36901 tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt tatcgatttt
    36961 tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt cgatgccgat
    37021 tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt gctgttcaag
    37081 tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta tgataggaaa
    37141 agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg ctgccggttc
    37201 taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt tgaacccttt
    37261 ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa tcacgatctg
    37321 ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt catgacagct
    37381 ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc ttccagctgg
    37441 ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt cagtgaagga
    37501 tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta aagctgcatc
    37561 tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg ttagaatagc
    37621 tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa gttcagccat
    37681 atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg cttcttcata
    37741 taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga atgctgtttt
    37801 catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca aaaaggccga
    37861 gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat tatgcagttt
    37921 gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct gtttcgattt
    37981 tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag ctgtcagctt
    38041 ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt cctaattctt
    38101 ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc gatattgttt
    38161 gctttttatt tttataattt aacagtctac aactccatga gaatacttcc ccatgttaaa
    38221 cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat ctttccacta
    38281 gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc atatttatcc
    38341 atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt ctcaataatt
    38401 ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat gacttcttgg
    38461 atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt catttgcaaa
    38521 taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg gtagccagcg
    38581 gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa ttttttctgt
    38641 ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc agtcgtacga
    38701 tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc cttagttgag
    38761 ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga gcacgcagca
    38821 ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg tgctgctaca
    38881 gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa atctttaggg
    38941 tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc agaagtctcc
    39001 tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca ttcatcagag
    39061 gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag gacgctggct
    39121 gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc ttgcaagaag
    39181 tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc cattccgtct
    39241 aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag taactttcct
    39301 tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat acgttcgaca
    39361 tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt aattgatagt
    39421 tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc gatcgtgtgc
    39481 atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc ttcgattgtc
    39541 aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc tggttccatg
    39601 ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac tatatcttaa
    39661 cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt tttttaagat
    39721 tttactcatt taaaacaact gataaatcag catttataat ttcttttatt agaaaaatca
    39781 tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat agaattccct
    39841 aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga
    39901 tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa
    39961 agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag
    40021 cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat
    40081 aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca
    40141 attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca gtaattataa
    40201 ttatgtacat attaccaata agggatttgt attaacagat actcctacgg ctcgaacata
    40261 cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg aagaaactta
    40321 tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc ttcctgacct
    40381 acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc aaggccgaaa
    40441 taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc agttgaattt
    40501 cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta ttagacggat
    40561 ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc cattagcagc
    40621 agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag aaacaatgat
    40681 tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc atttatcttt
    40741 gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca gttaccgcaa
    40801 aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag ctgaaaagca
    40861 actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg tcaattatcc
    40921 taaaatttat tggattaagt catagatttc catcgctacg atatcgatat tatcaaattc
    40981 gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt tatcataagt
    41041 tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt cattatctgc
    41101 tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat gtgtcatcgg
    41161 aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac ttgcattgat
    41221 tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct tcaggccacc
    41281 aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc aactcttcat
    41341 atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct gcttcttcta
    41401 atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg taatcttctt
    41461 ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca cctgaaagcg
    41521 tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc atttccgtca
    41581 ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc tgacctttcc
    41641 cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc agtacttgat
    41701 cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt gctcctaagt
    41761 caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct gtttttgcaa
    41821 ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt cctgagatat
    41881 ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg gggtaaccta
    41941 ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct gcccagatag
    42001 ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca gctacttcgt
    42061 gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt ccgataaaac
    42121 caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg ctagtatacg
    42181 ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa tgaatttaat
    42241 tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc ttcttcatga
    42301 taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt agatgaaata
    42361 tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga aatataatca
    42421 ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga aggatgatcg
    42481 aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc tgccatcgct
    42541 tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata atctgccgct
    42601 ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac aaattcttct
    42661 aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt tcctacgata
    42721 actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat caaggcttgg
    42781 atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt ttcctgaaga
    42841 cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg tttgaatttt
    42901 tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata gctgcgtaat
    42961 gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc aaataatcta
    43021 gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata atgaaggtag
    43081 tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt agctaccatc
    43141 ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag ttctagttct
    43201 ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc cttgaaagaa
    43261 ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc attaggatcg
    43321 gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt taatgttttg
    43381 atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa gccgattttc
    43441 atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt tgctagagac
    43501 tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt tattgatact
    43561 tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt gattggtaat
    43621 ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt tcttgctatc
    43681 ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt tgcttgctaa
    43741 agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga atagatctgc
    43801 acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca ggacattttc
    43861 aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca tgggaacatg
    43921 accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga aatcaatgat
    43981 tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg gagacagttt
    44041 gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct ctaagtcgat
    44101 cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca actgttttga
    44161 ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca gatatttttt
    44221 tacgatagga gaaagctggt tcgatccgat aaaggaattt cggaaatctt cctctgtaga
    44281 atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca tattatgact
    44341 attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat ctagtgtata
    44401 agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc cgattccaga
    44461 ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac gtcccccttc
    44521 acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct atttttgtct
    44581 attcttactg acaaaaacgt tttcttaaaa aagaacaaaa aaactggcgt agctctttta
    44641 caagccacac cagcttttga tacgttgttt attttacata ttcatgaaca agttcaacga
    44701 cttcttccat cgtatcgcat tctttcaatg cacgatcagc aagttcagcc attttcgctg
    44761 tgtccaagcg tttcattaaa ctacgcgttt taagaataga tgtcgcactc attgagaact
    44821 catctagacc catacctact agcaatggaa cagctgtttg atctccagcc atttctccac
    44881 acatacctgc ccattttcct tcagcgtgtg ctgcatcaat tacatttttg atcaaacgta
    44941 aaattgatgg gttatatggt tggtacaagt aagaaacgcg ttcgttcata cggtctgctg
    45001 ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa gaaatctact tcttttgcaa
    45061 acttgtctgc taagactgct gctgctggaa tctcgatcat gatgccgact tggatcgtat
    45121 cagatacttc cttgccttca cttatcaatt tttgtttttc ttcttcaaag atcgcttttg
    45181 ctgcgcggaa ttctttgagt gtagctacca tagggaacat gatacgtaaa ttaccatgaa
    45241 cagatgcacg aagcaatgca cgcatttgtg tacggaacat ttcgtcgcct tgttcagata
    45301 aactgatacg caatgcacga tatcctaaga atggattcat ttcatgaggt aattgcagat
    45361 atggtagttc tttatctcca ccgatgtcca ttgtacgaac aactacaggt tttccttcca
    45421 taccttctaa tacagctttg taagcttcat attggtcatc ttctgttggg aagtctggag
    45481 aatccatata caggaattca gtacggtaca gaccaactgc ttctccacca ttattatgga
    45541 caccaactag atcttttggt gttccgatgt ttgcagctaa ttcaaaatgc ttgccgtcag
    45601 ctgtgacagt ttctgcattt tttaattttt cccattctgc tttttgtgct gcgtaatctg
    45661 cacctgcttt ttcaaattca gctttttgtt catctgtagg atcgatgatt acatcgcctt
    45721 cgatcccgtt gacagccaaa atatcgcctt ctttgacttt agcagtaatt tcttttgtcc
    45781 caacgattgc tggaatttct aaagaacgtg ccatgatagc agaatgtgaa gtacgtccac
    45841 caatgtctgt tacaaacgct tttacaaaat tgcgatctaa ttgtgctgta tcacttggag
    45901 tcaagtcatg tgcaacaacg accacttctt cgttgatcat tgatggattt ggtaaagtaa
    45961 cgcctaataa gtgcgctaaa atacgtttag caacgtcacg aatatctgca gcacgctctt
    46021 gcatataagc attatcttcc attgcttcga acataccgat atacatatcc gttacttctt
    46081 ttagcgctga ttcagcattt acgctgttgt ctttaatatt ttgcttgatt tgacctacca
    46141 tttctggatc tgaaagaacc atcaaatggg catcaaatac ttgcgcttct gcttcaccta
    46201 agctttgcgc tgctttttca cgaatttgct gcaattcttc agtagattta gctaatgcag
    46261 catccaaacg agcttcttca gccgcagtat cttctactgt tgccttactg aatgttaaat
    46321 ccggttgaac tagcaggtaa gcttttgcaa cggctacacc gtcactagcg gcaatccctt
    46381 ttagcatttc aaccattatt cagataagcc ttctttcttc attgtttcaa cgattgctgc
    46441 cattgcatca gcttcgtcag caccttctgc agtaatagta acatcagaac cttgacctac
    46501 acctaaagac ataacgccca tgattgattt caagtttact gatttacctt tatactctaa
    46561 gtttacatca gagttaaatt tgcttgctgt ttgtactaat agtgtagctg gacgagcgtg
    46621 gatcccagtt tctgctacta cgtgaaattc tttcttttcc atattaatcg gtctccttta
    46681 agtgagtgtt gtttttatgt gagggttttc ttttttagta attcagagaa tcctaacaaa
    46741 tgccaccctt tcatctgtaa agattaacat tttttttcgt tagatacaac agttttcatt
    46801 caatttcaga taaaagtatt caaattattc cggattcttt ttcatccttt tcttcacatt
    46861 ttataacaaa cgtgtttatt tcctttttct tgtcgctttc tcttctcctg acgtttatca
    46921 ttctttcctt tgaccctctt tgttttcata tgaaacgaat tacttgcttc cctttttatt
    46981 tttagtataa taagagtcaa aggtcaggaa tggtcaatag agatttcacc ttttctacct
    47041 cataaatctc ttaaaaaact aggggttatt cttttacctc atgaaaaacg agatgtaaaa
    47101 tatggatgta cccatttttg tgaaaggacg tgaatgattc atgctttgtc aaaactgcgg
    47161 caagaatgag gcaacgattc acttgtatgc cactgtcaat ggtcagcgga cacaactcga
    47221 ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc tgactaatat
    47281 ggcccaaaat gatccatttg gatttggcag tttggacgat cttttccgtt ccatgtctcg
    47341 ccaaatgcaa cagcaaggaa attatgagca aacaccacca acacaatttg gcggaaacaa
    47401 caacttcaat ggtggtcagc cacctcaagg caatacagaa ggtttattgg gagaatacgg
    47461 aatcaatatc acagaacaag cacgacaagg tgatatcgat cctgtgatcg gacgcgatga
    47521 cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc aagaacaatc ctgtcttgat
    47581 tggagaacca ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa aaatcgtaga
    47641 tggtgatgta ccgcaaaaat taatggacaa agaagtcatt cgtttagatg tcgtatcttt
    47701 agtccaaggt actggtatac gaggacaatt tgaagaacgt atgcaaaaat tgattgaaga
    47761 gatccgtcaa gcagaaaatg tgatcctatt catagatgaa gttcacgaaa tagtcggtgc
    47821 tggttctgct ggtgatggaa atatggatgc tggtaatatt ttaaaaccag ctttagctcg
    47881 aggagaattg caaatggttg gtgctactac cttaaatgag taccgcatca tcgaaaaaga
    47941 cgctgcttta gaacgtcgta tgcaacctgt acgggtagat gaaccaactg tcgaagaaac
    48001 gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg taaaatatac
    48061 tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt tacgtccaag atcgcttctt
    48121 gccggataaa gccatcgatt tgctagatga aacaggttct aagaaaaact tgacgatcca
    48181 aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac aaaaagtttt
    48241 agcttctcga gaagaagatt tcgaaaaagc agcttattat cgcgatcaga tcaataaact
    48301 tcaaaaaatg aaagaacgtc agctcactga agaggaaaca cctgtgatta ccgaaaaaga
    48361 tatggaaaaa atcgttgaac aacggacagg tattccagtg ggtgaattaa aagaaaaaga
    48421 acagacacaa ctgaaaaact tagcagatga cttgaaagct catgtcatcg gacaagataa
    48481 cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt gttggattaa acaaacagaa
    48541 tcgtccaatc ggctccttct tatttgttgg accaactggt gtaggtaaaa cagagcttgc
    48601 gaaacaactt gcctacgaac tattcggttc acaagattca atgattcgtt tcgatatgtc
    48661 tgaatatatg gaaaaacata gtgtctctaa attgattggt tctcctccag gttatgtcgg
    48721 atacgaagaa gctggtcaat taacagaaaa agtacggaga aatccttaca gtcttgtctt
    48781 gttagatgaa gtagaaaaag ctcatccaga tgttttgcat atgttcttgc aaatcttaga
    48841 tgatggtcga ttgacagatg cacaaggaag aacggtcagc tttaaagata cgatcatcat
    48901 tatgacaagt aatgccggaa ctggtaaagt agaagcaaac gttggttttg gtgcggcacg
    48961 cgaaggtgtt actcgttctg tattgaacca attaaacaac tactttacac cagaatttct
    49021 gaaccgtttt gacgggatca tcgaattcag tgcattatca aaagaaaact tgatgacaat
    49081 cgtcacacta atgctagacg atgtcaacca aatgctggca gcacagcaac ttcacatcga
    49141 agttcctacg aatgtcaaag aaaaattagt tgacttaggc tatgatccat ctatgggtgc
    49201 tcgtcctctt cgacgcacga tccaagaaca aatcgaagat ggtatcgctg aattttatct
    49261 tgatcaccct tctatccatg agttgaaagc aaaactggat aaagatggaa aaatcatcgt
    49321 tacttctaaa ccagaacgtt tggctaaaga atcagccgaa gaaacggctg aataaatcca
    49381 gttttatagc gtgagacggt atttctttga gaaatgccgt ctcattcttt tttcagaaaa
    49441 gtcaaaatac tgttcatttt ataaaaaaga aattataata agagaaacaa gcaattaaaa
    49501 atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta acaaacagcc attcccgtct
    49561 tgtattaaac cagcttgaaa acactgatgc ccatctagtc aaggtctaca cagccggaaa
    49621 taccactatt gtctacactg aagcccctga acataatgaa attctgctga tcaatgacaa
    49681 acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat tatttcttag ataaaatcaa
    49741 agacgccgtt taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc ttgttgaaat
    49801 ctcgattcca aaacatgaat taggcgctac attagccgtg taaacaaatt ttgaacataa
    49861 taattaaaga gcgtgggaca aaagtaaa
//
LOCUS       DACEKQ010000008        48334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEKQ010000008 DACEKQ010000000
VERSION     DACEKQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.008"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..2095)
                     /locus_tag="IW671_000423"
     CDS             complement(23..2095)
                     /locus_tag="IW671_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0897439.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IW671_000424"
     CDS             complement(2028..2405)
                     /locus_tag="IW671_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0897440.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IW671_000425"
     CDS             complement(2534..3094)
                     /locus_tag="IW671_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0897441.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IW671_000426"
     CDS             3244..4536
                     /locus_tag="IW671_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0897442.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IW671_000427"
     CDS             complement(4596..5714)
                     /locus_tag="IW671_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0897443.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW671_000428"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW671_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0897444.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW671_000429"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW671_000429"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0897445.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IW671_000430"
     CDS             8920..9519
                     /locus_tag="IW671_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0897446.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW671_000431"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW671_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0897447.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IW671_000432"
     CDS             complement(11214..11558)
                     /locus_tag="IW671_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0897448.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IW671_000433"
     CDS             complement(11695..12831)
                     /locus_tag="IW671_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0897449.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IW671_000434"
     CDS             complement(13020..13994)
                     /locus_tag="IW671_000434"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0897450.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IW671_000435"
     CDS             complement(14074..14949)
                     /locus_tag="IW671_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0897451.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IW671_000436"
     CDS             15079..16986
                     /locus_tag="IW671_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0897452.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IW671_000437"
     CDS             complement(17527..20805)
                     /locus_tag="IW671_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0897453.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IW671_000438"
     CDS             complement(21076..21621)
                     /locus_tag="IW671_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0897454.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IW671_000439"
     CDS             complement(21633..22325)
                     /locus_tag="IW671_000439"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0897455.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IW671_000440"
     CDS             complement(22344..22628)
                     /locus_tag="IW671_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897456.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IW671_000441"
     CDS             complement(22641..23204)
                     /locus_tag="IW671_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0897457.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IW671_000442"
     CDS             23432..24094
                     /locus_tag="IW671_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0897458.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IW671_000443"
     CDS             24218..25414
                     /locus_tag="IW671_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0897459.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IW671_000444"
     CDS             complement(25515..26891)
                     /locus_tag="IW671_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897460.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IW671_000445"
     CDS             complement(26928..27794)
                     /locus_tag="IW671_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0897461.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IW671_000446"
     CDS             complement(27883..28908)
                     /locus_tag="IW671_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0897462.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IW671_000447"
     CDS             complement(28926..29600)
                     /locus_tag="IW671_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897463.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IW671_000448"
     CDS             complement(29784..30677)
                     /locus_tag="IW671_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0897464.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IW671_000449"
     CDS             complement(30751..32448)
                     /locus_tag="IW671_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0897465.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IW671_000450"
     CDS             complement(32441..33754)
                     /locus_tag="IW671_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0897466.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IW671_000451"
     CDS             complement(33754..37272)
                     /locus_tag="IW671_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0897467.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IW671_000452"
     CDS             complement(37565..38023)
                     /locus_tag="IW671_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0897468.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38297..38839)
                     /locus_tag="IW671_000453"
     CDS             complement(38297..38839)
                     /locus_tag="IW671_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0897469.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVICINCTNKA"
     gene            complement(38841..39458)
                     /locus_tag="IW671_000454"
     CDS             complement(38841..39458)
                     /locus_tag="IW671_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0897470.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IW671_000455"
     CDS             complement(39455..40309)
                     /locus_tag="IW671_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897471.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IW671_000456"
     CDS             complement(40306..41217)
                     /locus_tag="IW671_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897472.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IW671_000457"
     CDS             complement(41258..42592)
                     /locus_tag="IW671_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897473.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IW671_000458"
     CDS             42800..43630
                     /locus_tag="IW671_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0897474.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IW671_000459"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IW671_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IW671_000460"
     CDS             complement(44314..44508)
                     /locus_tag="IW671_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897475.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45013..45198)
                     /locus_tag="IW671_000461"
     CDS             complement(45013..45198)
                     /locus_tag="IW671_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897476.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46048..46389)
                     /locus_tag="IW671_000462"
     CDS             complement(46048..46389)
                     /locus_tag="IW671_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0897477.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46389..46619)
                     /locus_tag="IW671_000463"
     CDS             complement(46389..46619)
                     /locus_tag="IW671_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0897478.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            46906..47244
                     /locus_tag="IW671_000464"
     CDS             46906..47244
                     /locus_tag="IW671_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897479.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47449..47688)
                     /locus_tag="IW671_000465"
     CDS             complement(47449..47688)
                     /locus_tag="IW671_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0897480.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47664..48011)
                     /locus_tag="IW671_000466"
     CDS             complement(47664..48011)
                     /locus_tag="IW671_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0897481.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14069 a        10297 c         8454 g        15514 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaaca cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat acagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg ataatcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaaa agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tggaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt
    45241 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca ttatactgga
    45301 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
    45361 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
    45421 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
    45481 gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa aaactagcgg
    45541 tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg caaatttctc
    45601 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
    45661 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
    45721 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
    45781 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
    45841 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
    45901 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc atcgaccaca
    45961 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
    46021 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
    46081 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
    46141 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
    46201 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
    46261 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
    46321 tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
    46381 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
    46441 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
    46501 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
    46561 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
    46621 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
    46681 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
    46741 aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta
    46801 tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat
    46861 aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa
    46921 caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta
    46981 gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg
    47041 gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt
    47101 tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa atggcaacct
    47161 cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga
    47221 ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg
    47281 cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag
    47341 atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct
    47401 gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc
    47461 cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac
    47521 ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac
    47581 tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct
    47641 acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt
    47701 ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc
    47761 tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg
    47821 gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc
    47881 acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt
    47941 ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa
    48001 cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt
    48061 cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac
    48121 tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa
    48181 catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg
    48241 acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
    48301 aatgtagaaa gctattttca caaaattttt ctta
//
LOCUS       DACEKQ010000009        47591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEKQ010000009 DACEKQ010000000
VERSION     DACEKQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.009"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1455
                     /locus_tag="IW671_000467"
     CDS             352..1455
                     /locus_tag="IW671_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0897482.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1739..2419
                     /locus_tag="IW671_000468"
     CDS             1739..2419
                     /locus_tag="IW671_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0897483.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2509..3543
                     /locus_tag="IW671_000469"
     CDS             2509..3543
                     /locus_tag="IW671_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0897484.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKVETT"
     gene            3557..3892
                     /locus_tag="IW671_000470"
     CDS             3557..3892
                     /locus_tag="IW671_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897485.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3876..6338
                     /locus_tag="IW671_000471"
     CDS             3876..6338
                     /locus_tag="IW671_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897486.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGIPLQFDVTANVAKMTENGDKYDTSPN
                     KILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLNPGA
                     YAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGLTEG
                     RFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGLLSP
                     VALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRLYSK
                     GTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYPSFA
                     YKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENVTFT
                     LTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIGDTI
                     HLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPKQEL
                     TLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLFKND
                     QGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVNSST
                     NAQPGATYQGMVTWELTAGPTS"
     gene            6394..7953
                     /locus_tag="IW671_000472"
     CDS             6394..7953
                     /locus_tag="IW671_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897487.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7950..9314
                     /locus_tag="IW671_000473"
     CDS             7950..9314
                     /locus_tag="IW671_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0897488.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9485..10555
                     /locus_tag="IW671_000474"
     CDS             9485..10555
                     /locus_tag="IW671_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897489.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10570..11238
                     /locus_tag="IW671_000475"
     CDS             10570..11238
                     /locus_tag="IW671_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897490.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11258..11836
                     /locus_tag="IW671_000476"
     CDS             11258..11836
                     /locus_tag="IW671_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0897491.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12239..13150
                     /locus_tag="IW671_000477"
     CDS             12239..13150
                     /locus_tag="IW671_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0897492.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13406..14323)
                     /locus_tag="IW671_000478"
     CDS             complement(13406..14323)
                     /locus_tag="IW671_000478"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0897493.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14525..14962
                     /gene="zapA"
                     /locus_tag="IW671_000479"
     CDS             14525..14962
                     /gene="zapA"
                     /locus_tag="IW671_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0897494.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15020..15568
                     /locus_tag="IW671_000480"
     CDS             15020..15568
                     /locus_tag="IW671_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0897495.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15683..18043
                     /locus_tag="IW671_000481"
     CDS             15683..18043
                     /locus_tag="IW671_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0897496.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18130..18444
                     /gene="trxA"
                     /locus_tag="IW671_000482"
     CDS             18130..18444
                     /gene="trxA"
                     /locus_tag="IW671_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0897497.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18626..20404
                     /gene="uvrC"
                     /locus_tag="IW671_000483"
     CDS             18626..20404
                     /gene="uvrC"
                     /locus_tag="IW671_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0897498.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20549..21448
                     /locus_tag="IW671_000484"
     CDS             20549..21448
                     /locus_tag="IW671_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897499.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21441..22703
                     /locus_tag="IW671_000485"
     CDS             21441..22703
                     /locus_tag="IW671_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897500.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22864..24762
                     /locus_tag="IW671_000486"
     CDS             22864..24762
                     /locus_tag="IW671_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0897501.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25061..25780)
                     /locus_tag="IW671_000487"
     CDS             complement(25061..25780)
                     /locus_tag="IW671_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0897502.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26117..26449
                     /locus_tag="IW671_000488"
     CDS             26117..26449
                     /locus_tag="IW671_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897503.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26436..28439
                     /locus_tag="IW671_000489"
     CDS             26436..28439
                     /locus_tag="IW671_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0897504.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28458..28928
                     /locus_tag="IW671_000490"
     CDS             28458..28928
                     /locus_tag="IW671_000490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0897505.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28949..29533
                     /locus_tag="IW671_000491"
     CDS             28949..29533
                     /locus_tag="IW671_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0897506.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29548..30534
                     /locus_tag="IW671_000492"
     CDS             29548..30534
                     /locus_tag="IW671_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0897507.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30524..30835
                     /locus_tag="IW671_000493"
     CDS             30524..30835
                     /locus_tag="IW671_000493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0897508.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30857..32638
                     /locus_tag="IW671_000494"
     CDS             30857..32638
                     /locus_tag="IW671_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0897509.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32631..34007
                     /locus_tag="IW671_000495"
     CDS             32631..34007
                     /locus_tag="IW671_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0897510.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34014..34649
                     /locus_tag="IW671_000496"
     CDS             34014..34649
                     /locus_tag="IW671_000496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0897511.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34679..36034
                     /locus_tag="IW671_000497"
     CDS             34679..36034
                     /locus_tag="IW671_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0897512.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKFAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36226..37620)
                     /locus_tag="IW671_000498"
     CDS             complement(36226..37620)
                     /locus_tag="IW671_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0897513.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37712..38683
                     /locus_tag="IW671_000499"
     CDS             37712..38683
                     /locus_tag="IW671_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0897514.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38680..39461
                     /locus_tag="IW671_000500"
                     /pseudo
     CDS             38680..39461
                     /locus_tag="IW671_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(39630..40001)
                     /locus_tag="IW671_000501"
     CDS             complement(39630..40001)
                     /locus_tag="IW671_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0897515.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            40135..40578
                     /locus_tag="IW671_000502"
     CDS             40135..40578
                     /locus_tag="IW671_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0897516.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40621..41430)
                     /gene="yidA"
                     /locus_tag="IW671_000503"
     CDS             complement(40621..41430)
                     /gene="yidA"
                     /locus_tag="IW671_000503"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0897517.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41449..42816)
                     /locus_tag="IW671_000504"
     CDS             complement(41449..42816)
                     /locus_tag="IW671_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0897518.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42800..43630)
                     /locus_tag="IW671_000505"
     CDS             complement(42800..43630)
                     /locus_tag="IW671_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0897519.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43793..44230
                     /locus_tag="IW671_000506"
     CDS             43793..44230
                     /locus_tag="IW671_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0897520.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44306..44926
                     /locus_tag="IW671_000507"
     CDS             44306..44926
                     /locus_tag="IW671_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0897521.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45115..45621
                     /locus_tag="IW671_000508"
     CDS             45115..45621
                     /locus_tag="IW671_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0897522.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45638..46537
                     /locus_tag="IW671_000509"
     CDS             45638..46537
                     /locus_tag="IW671_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0897523.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46624..47509
                     /locus_tag="IW671_000510"
                     /pseudo
     CDS             46624..47509
                     /locus_tag="IW671_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT        15688 a         8203 c        10073 g        13627 t
ORIGIN      
        1 ttttgaacac cgttttatcg agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa
       61 aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttaata ccgagaaaat
      121 ggcttttgaa caccgtttat tcaaaaaaag ggactttgac actacttttg tcaaagtccc
      181 atcggttttt tatctatttt tattacaatc atttcttcca gagtagtgac atctggaatg
      241 gctgaaatgt aaacagatta tttgcctgta tcaatgtcga agagtaatat aaaaagtgta
      301 aacgctatca tattgttcat cattcaaatt ttatagagag aaggcattga gatggaaaaa
      361 acatatcaag caggtacaaa tgaaggaata gtagatttta tcaatatgga agatttagaa
      421 atcgcggctt cacaagtgat tccagctggt ggttatggtt acatcagcag tggtgctggc
      481 gacttattta cttatcaaga aaatgaacgg gcattcaatc atcgtttgat tattcctcat
      541 gtattaagag atgtagaatt acccgataca actacacatt ttgacgaaga aatgctgact
      601 gcaccaatca tcatggcacc agttgctgct catggattag ctcatgtgaa ggcggagaaa
      661 gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc ttacgcttct
      721 tgtactttag aagagataag agaagcaggc ggggagaaag caccgcaatg gtttcaattt
      781 tatatgagca aagataatgg aatcaatttg gatattcttg aagtggccaa gcgtaatgga
      841 gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga aacagaccgc
      901 agaaacgggt ttaccttccc gctgccaatg ccgatcgttc aggcttatca atcaggagta
      961 ggccaaacga tggatgctgt atataaatct tccaaacaaa aacttagtcc aaaagatgta
     1021 gaatttatcg ctgctcattc tgatttgcca gtctacgtga aaggtgttca atcagaagaa
     1081 gatgtctatc gatcattaga atctggtgct ggtggaattt gggtatctaa ccatggtggc
     1141 cgtcaattag atggggggcc tgctgcgttt gattcgttgc aatatgtggc agaagcagta
     1201 gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gtggacaaca tgttttcaaa
     1261 gcaatcgcat caggtgccga tcttgttgct attggcagac ccgttattta tggattgtct
     1321 ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga attggaaatg
     1381 gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa attgcgtgag
     1441 aatcgtttta tttaagaaat aaatgacgaa aaaaatagga gtgcggcaga tgatgccgta
     1501 ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga aaaaataaaa
     1561 ttgaaatatt gtttcatact taataaaaaa ggaagaatat tcatgtaaac gattttattg
     1621 tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga attcatgagg
     1681 attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt gatttattat
     1741 gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg gcttggcaac
     1801 accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta ctgaaccaac
     1861 aaataccgtt gagcctctta accctactga tccttcaaaa ccagtagagc cagcagatcc
     1921 ggaaaatcca gcaactggcc agacaggttc tttaacattg gatgtagttc ctgaattgcc
     1981 atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa
     2041 cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca
     2101 agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg
     2161 cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg aatccactgg
     2221 accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac caatatttac
     2281 agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag caaatatcac
     2341 actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt ggaacctcgt
     2401 ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga aaatactata
     2461 ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt ggtggacaat gaatcaaagt
     2521 aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt tccgtcaatc
     2581 agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc agagaaccaa
     2641 atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa tcagacacta
     2701 gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg
     2761 gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac gttagatgag
     2821 acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat caatgtatca
     2881 gcacatgcag aaacaactat acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga
     2941 cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga aaatgctaca
     3001 gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt acaagaaagt
     3061 caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga ggtcaacgca
     3121 aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag caatttgata
     3181 tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc taatgaattt
     3241 ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga taaagcaaaa
     3301 gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga atggcgattt
     3361 acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt
     3421 gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc agttctagtg
     3481 ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagt agaaacaaca
     3541 tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg caagtttgct
     3601 gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta cgattacatt
     3661 ttatgatcta agtccagttc cgcatccaag tggttcagtc aatggcgcat caaaacagca
     3721 gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg agacgaatga
     3781 agaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc ttgcgtggtt
     3841 gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat aaattttcat
     3901 tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc ttttcacctt
     3961 cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac acgtatgaaa
     4021 atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa
     4081 ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt tcaaatgatc
     4141 cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta acaaatgtac
     4201 ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg
     4261 aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc tacttgtata
     4321 ttgccggtgc ccccatcaag gttactagcg gtagtttatt atcagcagga agccatacct
     4381 tgcgttttgg gatggggtcg tgggatacaa atactctatt ggcagatatt attgctatta
     4441 ttacgcgagg tatcccgctt caatttgatg ttacagctaa tgtcgcgaag atgacagaaa
     4501 acggagataa atatgatacg agtccaaaca aaattttgac caagggtaaa ttacaacctt
     4561 ctatgtctaa aaaggccgtt ttttctgttg atttttatga ttcgaataat attatccaag
     4621 atcgtatgtt caattcgatt ttagctccag ctgcaggtag attttatcct aaattaactg
     4681 gcggaaactt gaatccagga gcatatgcat tttctgcaag tgcagagata aatacgtgga
     4741 atagttacat ctcaacaagt gattcaacag gaaaatataa tacatttgct gataccacag
     4801 aatacgtgga tggaaataac cagaaccttc caggctctac caatagtcgg acattgacga
     4861 tggggctgac tgaaggacgt tttgatcagt tgccatttag tcgctttgat cgcgtagtca
     4921 actattttac aggaaaaaac gttacacaag gagcaatact gactcatact ccagcaagca
     4981 caagtggatt gaatcagaca gccaatatcg tgtacagtgg gaaagatgcg caaggcggat
     5041 tgctttctcc tgttgcttta aaggtattgg agcagtacaa gctagacgga acggttttac
     5101 caacaaactt aacagccgat agtcagtggg gaaagctcct ttcttctgca tcgtaccaag
     5161 tcacggccaa ttggtcagca actgaactaa aaaagggttc gatccattac aggctttatt
     5221 cgaaaggaac ccaaaaatta gcgacaggat ggacagatca acttttccaa acgatccaat
     5281 caaataacgg gggtgtaaat acagctactt ctacactacc tgcattggct tcagggcaat
     5341 attattttga ttatcgcttg gtggacgatg tacttactaa catgtatccg tcttttgcgt
     5401 acaaatggca gtctgaacaa gctggaatca ccactcttcc tcaaataaca gtggctaatt
     5461 ttccatctgt gagtgcagat agtcagctaa aaaaccttag cagacaacct gagacaggag
     5521 atcccctgtc tgctctatca ggagatacga ttcaagaaaa tgtgacattc acgttaacaa
     5581 aatctgggga tgcgataacc gacaaaaaaa tcacgatctc acttcctgaa aatactactt
     5641 atatcaatgg ttccttaaaa ttaaatggta caactatcgc agataccggc attcaacaag
     5701 gagtttctgt cccagcagat ctattgagca aaataggaga taccattcat cttacttaca
     5761 actatcaatt aaatactgta gatacttctg tacaatccgt cagcatactg acaaaggcag
     5821 ctgtactttc cagcaatatt acattagcag atggtgctaa gcttcctaac ccagttgtcc
     5881 aaacatctgc taaaacaatt cttgtcccca aacaagaatt aacattagtt aatgtaccag
     5941 atgattttac atttggtaac gatctcccca aaccgttgaa gacaagttat tatgaagcaa
     6001 aaggcgattt ttcttttgat gtgcgggata ctcggcttcc aagcacgtct ccttggcaac
     6061 tgacagggac attgacttct ttatttaaga atgatcaagg tcaagaatta agcggaacaa
     6121 aattatactt caatcattct ggatcaaaac aattgattca gcaaggtcaa aacacgttga
     6181 tttatgagag tgatggaaca gctaaaggag aggttctcgt tgactttccc gataccgatg
     6241 gtttactctt agaggtaaat agcagtacga atgctcaacc tggcgcaacg tatcaaggca
     6301 tggtcacatg ggaactgaca gctgggccaa ccagctaagt gattagaatt tgtatggatc
     6361 tgtgataact gtcacagatc ctctttttca aggatgagaa ggaaacttct gctgttaaaa
     6421 tcagaaatgc ttggaggagt ggacttggaa cttttcagtt tgcttgaaaa aaatgaacaa
     6481 cggcaaatca aacttttcca acttttatta gaggaaaaag gagagatttc cgttaataac
     6541 gtacttaccc gcttatcagt tgatcgggct accttgaaag aagatctgtt ttatttacaa
     6601 gctaacttgg aaaatatcaa acaattggaa attgttatcc aagaagggaa tattttatta
     6661 atcagacatg gaaatatagc agcagtcgat gtttattatc attgctactt gcgctattct
     6721 acaaaatatc agatacttct ttatctgttg gaaaatggct catttgatcg gcagaaactt
     6781 gtcatggaac ttaatatcag ttcggctact ttgtctcgca gagtgaagga attaaatgat
     6841 tctttaaatg agtttcatct gaaaataaaa agcgggcggc tgaccggtcc agaaagtcaa
     6901 atttgttatt tttatttcca attattttgg tttggacgtc cttatcttga aaacaaaaaa
     6961 gaatacagtg atttatcagg aaacactttt ttagatctgc tgcaaaaaga gtttagtttt
     7021 actattacag aagaaggacg attgaaattt gaaatatggc tttccattat gaagaagcga
     7081 atgcgctgta cgaaaaaatc caaaccagat tatagcaatc ttcctgttgg attgtttcaa
     7141 acgacacaat ttttattgga cctccataca tttttgaccc gttacttctt tcaatatgcc
     7201 tttccttgga atcaaaatga aacgttggtt ttttatcttt tcatgatcag taactttaca
     7261 ttagatacac ataactcgca tgtccaggag tttttgataa gcgaaaaata caaagacgac
     7321 gtagtagctc aattgaatcg aacgtttaaa caatcgatag aagaatattt ctctttttat
     7381 gtttttccag aagagatttc gcaaaagatt gcgttgacag tgatgcaaaa tcacgctcat
     7441 tttgcctatt ttagaggatg gatgaatatt tttggccaag aaggattaaa acagcggtta
     7501 actgaagtag aagataaaca attcctggtt ttatgtgaaa gattggtcag acaaacattt
     7561 gctattgttg attttactga agtagacgag gaaaatgccc gctttgaatt atttgctcgc
     7621 tatgcatcta ttttgcggac gctgatcaag cagttagaat tacgtctgca aattgcttat
     7681 gatcttcctt atgaaaggct aatggcggat atcgtgaaag aaatcatcca ggaacaattg
     7741 ctgaatacga tcaaatatga tttagtcgaa tacgagaaag aaaagcagta tgatatcatt
     7801 ttgactagcc aattgaaaga gtacccgtca cagaagtccg ctaaagtttt tgtattcact
     7861 agcaacgaat ataattatga tttcccctat ttgaaccaat ttttaaaaga atgttattta
     7921 gaaaaattga atttaaggat gaataaaaca tgaaaatcgt gatcattggt gcttcccatg
     7981 caggcatcac agctgcccta aatctaagaa aacttcaacc gaaagcagaa gttttactga
     8041 tcgatgaaga tcataaagat ggactaggct atgtttcaaa tggaatcaac ctttatttaa
     8101 aaggaaagat acgtagttta gctgaagttg cccataacat gcggactctt caagaaagtg
     8161 gggcaaaact gatcaccgaa tggagagtaa ctgaacttga tccagacaaa catcagctca
     8221 tcctcgcatc caaagaagga aaaaacgaga ccataaccta tgataaatta atcgtagcaa
     8281 caggttcctc tcctgtcacc ttgtataaac aaattgaagc tgaaaatgtc tacacctata
     8341 aaaatctagt gcaatctaag caagtacttg ccgctttaaa agaagcaaaa gaagtagtga
     8401 tcttcggtgc tggttatatt ggattggaat tagcagatgc tttgagaaat aaaggataca
     8461 ttattcatct agttgattat atgccgaatg ttttatcaag atatttcgat aaggatatga
     8521 tcaacagttt tcaaaatcag ctgcaaacta agcaaatcaa tttttatcca aatgaattct
     8581 tgatcgattg gaaaaaatca gaagagaaag tagtatccgt acaacttcta tctcaagcaa
     8641 taaaggcaga tatggtcatt ttttctgccc aaactcgtcc taatacgaca ttgttaaaag
     8701 aaaaagtggc tttgtatgag gatgagactg tgatagtcaa tgaatatctg cagacaagtg
     8761 atccggacat ctatgcgatt ggagatatcg tcccagtcag ttttgacaaa aataaacggc
     8821 atctcttttt acctttagtt actcgagctg tacatatggc aagagcagta gcgctaactt
     8881 tgagtggtca gccaaccgct tacgacctca gacaaaagat aacagcagca gtcattatag
     8941 actacttttt aggaactgta gggctgacag aagatgaagc gccattcttg gaacaaaata
     9001 ccggttcgtg ttcaggagaa tttgatctat ttccacaata cgatgaagaa aacaaaacag
     9061 taaatgcaaa gctgatctac catccggata cattagaaat aattggcgga cagctgataa
     9121 gccaagaatt cttattatca gatttgaacc tgcttgcgga tatcgtgaaa cacaaaacta
     9181 cgattccaca gctagcagtt gaggattttg gatttctgtc tgaacacacc tctcgttttc
     9241 actacctcaa tgagttagct tttaaagtac tagctaagag agcaaatagc aatagagtag
     9301 ataaaagact gtgagaaata tcacggtctt ttattgtgtg gggatataat aaatattaat
     9361 caatcgagaa taaaaaattc atataatgaa atagcggaag aattttttga gcataatatt
     9421 cgccaaaaat ttatcaagtg ataaaattgt tgtataaaat gattatttca ggaggaggag
     9481 ttaaatgttt ttagcattaa atgaaattat gcattcaaag ttacgttatg cgttagttgc
     9541 aggggtgatg tttttgatcg cctacctggt attttttttg actgggttag cttatggctt
     9601 agctcaagat aatcgaacag cagtcgacaa gtgggaggca gattcgattg tgctttcgaa
     9661 agatgcaaat tcaaatctag ggatgtccat gattactaag aagatagcag aagaagtcga
     9721 aggtgggaaa gtggcgtatc ttgcccagac acctggagtc gtgactagta aagacagtac
     9781 agaagaagga aaaatcaatg ttagcttctt cggtatcgat aaaaatcaat tcatcatgcc
     9841 caatcttgtt gaaggaaaag cttttgataa cgatgatgag gcagtaggcg atattagttt
     9901 gaaagaagaa tatggtctag ccgtaggaga tacagttaag ttatctggta gtgacaagac
     9961 gtttaaacta actggcttta cggatcatgc caaattcaat gtctcaccag ttctctatac
    10021 aaccatcaat gcttaccaag agatccgctt cgaaaaagaa gatacaagtg aaaatgcacg
    10081 gattaacgca atagttgtca gaggtaaaat cagtaattta cctgaagatt tagaacagat
    10141 caaaatttct aaattcatca atgaattacc tggatataat gcgcaagtat tgacttttgg
    10201 atttatgatc ggatttttga tcgttattgc ggctattgtg atcgggattt tcatttatgt
    10261 actgacgatg caaaagatta atatctttgg tgtaatgaaa gcacaaggga tcacaggagg
    10321 atttattgcg cgttctgtag tggcacaaac attcatttta tcatttgttg gtattcttct
    10381 tggtctatta ggaacagtgg gaacatccct tgttctacct gatgcggttc cattccaaag
    10441 caattggctg ttttttggcg tcattagctt gttgatgctg gtagtagctg tgttaggcgc
    10501 actattttct gttcggacaa tcgtaaaaat cgatccgttg aaggcaattg gataagaagg
    10561 aggaagagca tgaaagcaat agaatttaaa gaagtagaaa aaagtttcca agatggcgac
    10621 caagtagtta aagcattgaa agaaacaacc atgagtatcg ataaagggga atttgttgca
    10681 atcatcggtc cttctggttc aggtaaaagt acttttttga ctttggcagg cggattgcag
    10741 acgcctagca gtggctctgt atttatcaat gatcaagaat tcagtaatca aaaagaaagt
    10801 gaacgcgtta aactacgttt taaagaaatt ggctttattt tgcaagcttc caatctggtt
    10861 ccttttctga cagtaaaaaa gcagttatta ttagtagata agatcaaaca tgaacatcgt
    10921 caaaaagaag caaaagaatt atttgagcag cttggcgtgg ataagttgat caacaagtat
    10981 ccagaagaac tatctggtgg agaacggcaa agggtcgcaa tcgctcgtgc attgtacaat
    11041 gatccgtcca ttattctagc agatgaacca actgctagtt tggattcaga aaaggcacga
    11101 gaagtagtcg atatcttggc aaaagaatca aaagacaaaa ataaagcaac gatcatggtt
    11161 actcacgata cacggctgat tgacaaatgc gatagaatat ttatgatgga agatggtgta
    11221 ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg gtccatcgat acaacaaaga
    11281 tacacagcaa aaaattttga aagaagcgac tgacttgttt atgtccaagg gatatctcgg
    11341 cacttcaaca agagagatcg cacaaaaagt tgggatcacc caacccaatc tctatcatta
    11401 tttcggtgat aaagaaaaac tatatacggc agttttagaa aaccacttaa aagatgttgg
    11461 aaaagctttg agagaaatcg ttcaaacaag cgaaacggga tttcaagtaa ccttgacaaa
    11521 aatggcgcag tttttgattg agacacatct ggtcgatttg tttctgatgc ttcacgatct
    11581 tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc tttctatgga agaaaaatta
    11641 tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct gtgctaagaa atgggatttc
    11701 aaaagaaatc gcagcaaggc attttttctt agtgttagca ccttacatca ctcggtcgaa
    11761 aagacctgtg gaagaaccgc taaatgtggc gcaattgatc gatttatatt tgaatggtgt
    11821 attaggagaa gcgtaaagct gttatatagg gagaaaaata gttaggtgaa gaagcaagat
    11881 atgacgaaga tcgttatatc ttgttttttt tatataggta gtttttcata aatacaaatt
    11941 tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg ttccaggaag
    12001 tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac tatttattgg
    12061 gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa
    12121 atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc ttttttgcgt
    12181 acttaagctg attttaagtt ttgaccctta gtctggatct acgaggtgag agtcaagaat
    12241 gaaaaaaata attggagctt ttgtatttgc aatcattatc attattatct gttttcaaat
    12301 agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga acagtacggt
    12361 tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca aaatcggaaa
    12421 aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa ctataaacgg
    12481 cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca agacaaatga
    12541 tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac
    12601 aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg gcggaaatga
    12661 tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat taagtacagc
    12721 cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga cccatactac
    12781 agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa aggcagatgt
    12841 ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg gaatcacgac
    12901 atattattac tatggacaag aggaaacatt agcacagaca attgctgact ccttcactga
    12961 tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc gagaaaatct
    13021 tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt taaaggaatt
    13081 agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat tgaccactta
    13141 ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt tcgtttggcc
    13201 gggcttatta tcgcagatgc aagcttttga acaccgttta aggaagaggt tgtgagcctg
    13261 ccgtttaact ccgcgtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgatgact
    13321 attgtgactt gtgaccgagg agttggcatg agaacaccgt ttattcggaa caagggactg
    13381 tgacatgact tttgtcacag ttccattatt tttttagcag cttcaaagct ttttctgtat
    13441 tggcaaaatg aagtttggca tatttttcaa gcataggcat gccgcctttc tccaatagtt
    13501 ttgccgctac ttggtcagat ttcgttccag ctcctgatgg aagggtgatt ccagcttcag
    13561 ctgatgcttt gtgtaactct tccaaaaagc tggcacgact gatgatagaa gcagcagcaa
    13621 cagctaaatg atattgttct cctttagtaa cgaagtacag tttttcttgt aattgatttt
    13681 tttctaaacg cgcatatttt ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg
    13741 cttctggttt ttctggctcg atttttttta gcagcaaatg gatcgcttga ttgtgcaaag
    13801 cgactttcat ccgaacggca ttgtattttg gttggatctc attgtatttt tctggagata
    13861 agacaagcag tttatagggg atggtttttt ttaagatgtc agcaagctgg atgatttggc
    13921 tatcttttaa ctcttttgaa tctctaacac caagtttacg taattctgca agctgatcac
    13981 gtttcacata agcagcacat actgttagcg gaccaaaata gctgccattt ccgacctcat
    14041 cactacctaa aacggataat tgactgatat tttttggcaa agaagaggct gtttttgctg
    14101 gcttggcttg tttatcctgt gcctcccccc atttatcagc ttctattgct gcattcgtac
    14161 cttgaaacat cacttttccg gatgtataag cggtaatggt cgtcccgtct ttttttgcag
    14221 caaagctagt ataaggaatc tgtttattta acaaataaga gcgatagtat gctttcattt
    14281 gactaagttg gtcgtttgtt gctttgataa tgacgttgct cataaaattc tcctcgtttt
    14341 tatacataaa actataccgc aactcacgac ttttcgtttt ctaagagatt gcgagacata
    14401 gcattttttt aaagtttatc atataatagt taagtatgtg aaaaatattt gtggagaacg
    14461 gtgaaagaac caacttcatg gtacactaaa taaaactttc atcaaatagg aggaaatgat
    14521 tcgtatggcg cacgaaaaaa cacgctacaa agcagtgatt gccaaccaaa catatactat
    14581 tatcggacgg gaaaccaagc atcatatgga tatcgtaacg aagctgatca atgaacagtt
    14641 agcagaattg aaacaattat caccacaaat ggataatgaa caagcagcaa tcttgatggc
    14701 tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga atcttggagc tggaagaaga
    14761 aacagcagaa ttaaagaaaa aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg
    14821 cattgaagca atcgagaacg aagctcggga agtattgaaa gaaaatggtc aagcagatca
    14881 taccattaaa aatcatgtgg aagcacaaca gattttaaac gagaaacgaa aaggacaaat
    14941 taaacaaaaa tcttctaatt aagatccttt tatcaaaata agaaaaattt aaaaaagtta
    15001 gaagagaaag gaacgtagca tgctgagttt gctgatttta tttttattgc taattgcgtt
    15061 tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt
    15121 gtcatttatt gctgcccagc atttttacaa accattagca agtcatttgg aattatacat
    15181 tccataccca gctgtcaccc caacatcaaa gttagcattt tttgatcagg tatttgcttt
    15241 tcacctagat gaagcttttt atgcaggaac tgcttttctg attattttgc taattggctg
    15301 gctgcttacc cgatttgtgg gggtttttgt ccatggattg acgtatgtgc cgatcttgag
    15361 acaggtagac tggattgcag gaggcatcct gtcactaatc atggcttacg ttactatatt
    15421 tttgattttg cagttacttg cctttgttcc attagatgtg gtccaaaatc aattcggcga
    15481 gcatagtttc gctcgtttta tcgttgagag aacgccattt cttaccaaca agatgtatga
    15541 gctttgggta acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt
    15601 gtacagagta gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca
    15661 tagtgaaaga taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa
    15721 agtcaaacag atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga
    15781 attggtacca gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg
    15841 attgaaagtg caacgtctgc gaggcgggat ccctatacct aaacttgaaa atatccgtcc
    15901 ccatatgaaa cggatcgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc
    15961 cagagtattg tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga
    16021 aatcgaattt gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag
    16081 ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga
    16141 gttaaagagc ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga
    16201 tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa
    16261 tgaccgctac gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca
    16321 cgatcaaagt gcttcaggac agactttatt tattgaacca aaacaagtgg tggatttgaa
    16381 taatcgtttg cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga
    16441 actttctgct gaacttgttc cccatcgtca agaaatcatc cacaatgctt atgtgattgg
    16501 taaaatggat ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc
    16561 gggaatcagt gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca
    16621 ggaaaaagta gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac
    16681 aggacctaac acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat
    16741 ggggcaagca ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga
    16801 agtatttgcg gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc
    16861 tcatatgacg aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt
    16921 tgacgagctc ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt
    16981 agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa
    17041 agtttatgga tacaatcggg caaatacgat taatgccagc atggagttcg atgtggatac
    17101 actaagtcca acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat
    17161 ctctagccga ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga
    17221 tgagagccaa gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga
    17281 gaccgaatat ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt
    17341 gaaagaagct tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa
    17401 aaaagcaaac gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat
    17461 tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc
    17521 aaagacccaa ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa
    17581 gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg
    17641 ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt
    17701 aaaaatgaat gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca
    17761 acgagttatc catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct
    17821 tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat
    17881 tctagctggt tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa
    17941 gggaattact gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc
    18001 aaaccaaggc ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag
    18061 cagatggttc gctatccaag tatagaaatg ctatactaat catagatttt aaaaatggag
    18121 gtctttgata tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga
    18181 cttgtattga ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt
    18241 ttggatcagt tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg
    18301 gatgaaaatc cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg
    18361 aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt
    18421 caaatgatcg atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga
    18481 atccagttcc gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt
    18541 tgagtataat tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt
    18601 aattgaatat gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact
    18661 tcctgatcag cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg
    18721 caaagccaaa atattaaaaa atcgtgtccg ctcatatttt actggcagcc ataatacgaa
    18781 aacggaacgg cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat
    18841 cgaagcattg ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat
    18901 gttaaaggat gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt
    18961 ggtgattact cgtaaagtac taaaagacaa agcactgtat tttggaccat atcccgatgt
    19021 cggtgcggcg aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa
    19081 acctagtcag acgaaggagc cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta
    19141 ttatttcgac gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaagttttt
    19201 aaacggagga cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga
    19261 aaacatggag tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat
    19321 catgactcgc caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc
    19381 gattgacaaa ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga
    19441 acgagatgtt tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct
    19501 cggtcagttt tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac
    19561 gatcgacaag cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg
    19621 agaaaagaaa aaattagttg aattggcgaa taagaatgcc tcagttgctt tgaatgaacg
    19681 gtttgatctg attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa
    19741 cgcgatgaat atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg
    19801 gacgaatcct gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata
    19861 tcggaaatat aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt
    19921 catctatcgc agatattcac gagtgattcg cgaagggttg cctttacctg atttgatctt
    19981 gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg
    20041 aatcgatatt cctattgctg gcatggcaaa aaacgataaa cataaaacaa atgagttgct
    20101 attcggtata gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact
    20161 ccagcgaatc caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag
    20221 taaaaacagt tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa
    20281 aatgctgctt aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt
    20341 acaagaaatc ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga
    20401 atgaggaaag ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa
    20461 agagcgttta cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata
    20521 tgtgatttct tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt
    20581 tgggaactac acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg
    20641 gctaattggc caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt
    20701 gacacaagac caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga
    20761 tgtcatcggt tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca
    20821 actgctttat tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga
    20881 tttttggatg gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc
    20941 taaaggaaat cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat
    21001 cattttggat gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg
    21061 aatattggaa ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa
    21121 tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg
    21181 aaaagtacat gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt
    21241 aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa
    21301 tacgttagag attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac
    21361 tcagttcggc tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa
    21421 attgaaagca ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa
    21481 aaaatgttaa gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta
    21541 tcatttatgt agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat
    21601 tggctgataa tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt
    21661 tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg
    21721 cgtatttagt agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt
    21781 cattaggaca aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc
    21841 ttcgagcaag tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa
    21901 gtttgactcg acaaaaagtc aattttgatt caaaaggaaa aatgacagtt ggagaagata
    21961 actcgacagt ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc attattattt
    22021 tgacgtatgc gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg
    22081 aagtgatcct gtccagtacg actgcacaaa agcattttta cggaaaacta acaggtgttt
    22141 tacttgttgc attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc
    22201 aattcaaaga tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga
    22261 ttggaccatt cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg
    22321 ttttagcagc tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt
    22381 tgccagtgac ttatctatct ctaggcggat atatgcttgg attgatacta ggagcttcag
    22441 atccaaacaa tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta
    22501 tgccggttcg cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg
    22561 tacttgtagc cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg
    22621 tactcgtgta tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga
    22681 gaaatgaacg aaaaaaagca taaaatatag aaaaatatag aaggaagctg gacatttatt
    22741 gttcccagct tcttttttgt atagacctat ttctattttt ttattatatt ggatatatat
    22801 tcattgaaaa tagagcataa caactttata atgttgagta taccaatttt tggaggcgtt
    22861 ttaatggacc gctatttaga tttactttca gagaagttcc ctacaacgga agatgtcatc
    22921 acggaaatca tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg
    22981 agcgatatac atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat
    23041 cttaaagaaa agatcaaaga ccttttctct gaacaaatga cggaaacgga gatcagccaa
    23101 ttcactattt ttacggctta tccagaatat gtgttgacga ctgattggta caaaaaacaa
    23161 gataaatccc agctgatcca tcgtttgatc gatcttttac ggtttacgac agtcaaatac
    23221 actcgatcaa aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc
    23281 ttgtatatcg atgatcgtat caacgggaaa aaagactatg taaaaaaaat cattgaacaa
    23341 cttgtcttga tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac gatccaaaaa
    23401 tctgtcatcg atcatttgca tatcgtgggt gatatttttg atcgaggaac tcaagcagcc
    23461 aaagtaatgg atcgaatcat ggatttttcc tctgtagata tccagtgggg gaatcatgat
    23521 attttgtgga ttggtgcgta ttgcggctct gaggcatgtt tattgacgct tttacggatt
    23581 gccgcacgct acaactattt atttgaatta gagaaagaat atggattaaa tttacgtcca
    23641 ttattttctt ttgcccatca aacctatcag aagaatccag tatttacccc taaaaacagg
    23701 aaagatctat ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc actttctatc
    23761 ctccaattca aatcagaaca tcagttgttg aatcgacgac ctgaatttaa aatggacgat
    23821 cgtaaattgt tggaaaaaat caactatgag gaatcaacga tcgttttaga aggtcagctc
    23881 tatgagttga aagggacttg ttttcaaacg atccagccag atgatcctaa aaaattcttg
    23941 gaagaggaac aaaaagtgat cgatagtttg atgtattcat tccagcactc gttaagaatg
    24001 caaaaacaca tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat
    24061 gtactgttcc atggctgtat acctgtagat gaaaaggggc agctcttagc atttgaatta
    24121 gaccaatctt atcgcggaaa agaactgctg ggattttttg aaaaacagat aactgaaagc
    24181 gcaaaagatt tgactgcaaa tgaagatttg gcgaccgatt tgatttggta tgcgtggagc
    24241 gggccatttt cgcctttatt cggcaagagt aaaatggcaa cttttgagcg ctattttctc
    24301 acagagcctc atacgcacgt cgaaaaaagc aatccctatt atcacttgcg agatgaagag
    24361 tggttcagtg aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc agcaatcatc
    24421 aatggacaca cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc aaacggaaaa
    24481 gcatttgtta ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg gattgctggc
    24541 tactcattac tatgcaattc ttacggtttc caaatcgtga cacattatcc gttcttgcct
    24601 attgaacaac aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt gatcgagcag
    24661 cagctcccac gcaaacttaa tcggaatacg acaaaaggaa tggaattaca gcaacagatt
    24721 actcaactcc aaaggctgtt ggattatcga aaggacgatt gagaaaaatg ctagccaaca
    24781 ttgctcaata gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc cttcggaatt
    24841 agctcaaaaa acggtaaaat attagaagct atttttctgt ttatttctct aatactcagg
    24901 tcagggcgct gatttcacga cctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    24961 acaagtcaca atatccaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    25021 agagttgaac ggcaggctca cgacctaatc agaaaaacga ttaaaattca ttcacaaaga
    25081 aatcgaataa acgctgttca gatgaatcta cacgatgaga aagttccgga tgccattgga
    25141 caccaaggat tttgtgcata ttatctttac tttggactgc ttcaatgagt ccatcgttgg
    25201 aaaaggcgat ggcttttaac aaaggagaaa gatctttgac tgcctgatga tgataggaat
    25261 tcacaaagta tgaatcaggg agaaggtcac ttaataaact gtccgaaacg attttgactt
    25321 cgtgagttgc aaactgcggg gcagtgggct gttgttcgtg ttttacggac cattttggat
    25381 ataatgaaag atcttgataa agtgttccct ccaacacgac attcaatagt tgaagtcctc
    25441 ggcagactgc aaaaatcggt tttctttgtt tcaatgcttc caagatcaat gcctgttcga
    25501 attggtctcg ttgaatattt gtttccgtca gttttggatg agggtcttcc atgtaaagtt
    25561 gcggactaac atcctgaccg cctgcaagaa tgagtttgtc gattttctca attagttgag
    25621 gcgctgaatc tggagacatg actggcagaa taagaggtaa tccttgagca tctaaaactg
    25681 catccacaaa tccctgcggt gtatagctta cttgatttcc ttggaaagta tctgtcgctc
    25741 gaatcagttg attcccggca atgccgatga ttggtttcat ttttattccc cctaaagtag
    25801 actaattgta acaaggattt aaaaaaattt aaactatgta agacttgccc ctatacttat
    25861 ctgttcttgc tgtgttacta tctgtaaaga gaattttcgt gtgaaattca tgacgatagt
    25921 tgaaaatatt gcttaatttg ttataataaa aagtaagcag aaataaaaag gttgggcgta
    25981 agcttcaagg atctagttct tcaaggggtg agaaggacat tttattgaag gggcctgcct
    26041 atactaagtt atctttagag gtacatactt tcaatgaaaa aagaaagcat gtacctcttt
    26101 tttaaatcga aaggaagtga agcaagtgtt agaagctttg gaacaaatcc gaaaagcaga
    26161 agaagaaaat gagaagcgtg ctcgaaccct acggcagcag ttgaagacct atgcagaaga
    26221 aaaagagcaa gaattggaga taatccaaac gaacagtcgg caacagctga aacagttggt
    26281 agaagaaaaa gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga aattgaaagc
    26341 agaagcagac aaggtgcaag caactttgaa agaccaatat caaaagcatc agagtcaagc
    26401 aatcggtgca atcattgaaa gggtgaaaga aacttatggc cgtcactaag atggaaaaag
    26461 tgaccctcat ttcagataaa agaaatcagg agaggatttt acaagcagta caaggaatcc
    26521 aaaatgttga aatccgggat ctgtatcaaa atagaacaaa caaccagtgg gtagaaaaat
    26581 attttccaga taccacgatt attgataaag aagcaaaatt aagcgcttta ggcaatcggc
    26641 tgaaagacat ccaagaagct atccaattca tacaacacca cggcgatagc cgtcaaaaga
    26701 aactccattt gaaacgtcaa gaactttcct tacaagactt ggaagcaaat tattccgaag
    26761 aaaatttcat gaataaatta gaagaggtcc ttgatttaaa agaaaagtgg gaacagctaa
    26821 ctgaaaaacg tgatcgttta gaagaagagg aagaatggct tcttcattgg caacagctgg
    26881 atattgctcc aagtatgtat gcaagtaaga cgaccatttt tgagatgggg actgtgagtg
    26941 cagcaaattt tgaaagtttc aaggaagaac taagtaagct tgatgacgtt tatatggaag
    27001 aaatagatta tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa aaatcagttg
    27061 ctgctataga agaaattgct gggaaatacg ggttcaataa agaaacgtat ccttatgaac
    27121 aaaatcctaa agaaaaattg caagaaacca aacagcaatt atccattgtt cacgagcagc
    27181 aaaaaaagat aacgacagcg ttaggtcagt gcagcggcta tatccaagaa tttgaatggg
    27241 tagaagaagt cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg tttatccatg
    27301 ccacttatct gatcgttttg caaggttggg tagatgcaga agaaaaacag aatttgatgc
    27361 atgtgttaaa cgaaactgta tcagaagaag atatatacgt tagtttcgaa acaccaaact
    27421 cagaagagat tcagcaggaa gtccctacta aattgaaaaa ccatccgctt atcgaacctt
    27481 ttgaattgct gactgaaatg tacagtctgc caaaatatga agaagtagat ccaacgcctt
    27541 ggatgacacc attttatctt gtcttcttcg ggatgatggt cgcagatgtt ggttacggat
    27601 tgctgatgct cattgggaca atactagctc aaaaactatt tgttttaccc cgcgggatga
    27661 aacgtttcgt aaaattcttt gagattctct cggtaccatc gatcatctgg ggattgatct
    27721 acagttcatt tttcggacag gcattaccaa aagaactatt tggtatccgc ttgcctttcc
    27781 caatcctatc tacgacggat gacgtcaata cgattttgat tttatccgtg attttcggtc
    27841 tgatacagat cttggttgga ttattcgtgg cagcaaagga aaatatcagg aggaaagatt
    27901 acttgagtgc tgtcagtgat ggttttgcct ggcaagggat cctgattggg atcgttattg
    27961 cattgattgg tgtcatgttg ataaaaaata cagcatttgt ttatcttggc gcggggctat
    28021 cgatcattgc tgctttatgt atcttaatcg tgccgattat ccaatcgccg tctaaagtca
    28081 agggtgctgc caaaggagcg tataatcttt acggattgac tggttatatc ggtgatttag
    28141 tcagttatac acgtttgatg gcactaggta tttcaggcgg aagtattggc gcggcattca
    28201 atatgctagt tgctttcatg ccgccagccg ctcgattcag tgtagggatc cttttgatca
    28261 tcgctttaca cgcactgaat atgtttttga cactgttaag tgcctatgtc catggtgcac
    28321 gattgcagta tgtcgaattt tttggaaaat tttatacagg aggcggacgt gcctttgacc
    28381 cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac aataaataat
    28441 tggaggattt ttatacaatg atggattact taattagtca aaatggtgga atggtatttg
    28501 cagtattagc gatggcaaca gcgacgattt tttcagggat tggttcagct aaaggtgttg
    28561 gaatgacagg tgaggcagca gcagcgttaa caacaagtca acccgaaaaa ttcggacagg
    28621 ctttgattct gcaattgctt ccaggtacgc aagggttgta tggattcgtt atcgcctttt
    28681 tgatttttat caacttagga aatgatatgt cagttgtaca aggattgaac tttctaggcg
    28741 cttctctgcc gattgctttt acaggtttgt tttcaggtat cgcccaaggg aaagttgcag
    28801 cagcaggtat ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga atcatttttg
    28861 ctgcgatggt tgaaacatat gcgattttag gtttcgttat ttccttccta ttagtattga
    28921 atgcctaagc ataaaggagg aaaagcagat gaacgccatt gaaaaaatca tctctcagat
    28981 gaatgaagca gcagagcaag aaagagcggc gttagagcaa gaagagcgaa tgaagatcga
    29041 tcaaaacttt gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac aaaaagaaaa
    29101 acaaatcgaa ttacttgaaa aaaaataccg tcagcttagg aatcgccaac aagtagaagt
    29161 acgacaagaa aatttgaatg cgaagcagga atttttacga cgattgtttg cagatgcaat
    29221 agctgaaatg gagaattggg atgaatctga acaaatccag tttatcaaaa attcattgta
    29281 ttcattacct ttgacaggca aagtaacatt cattgctgga gaaaaatcag cagcttatct
    29341 ttctcagaca ttgctggatg aatggaacaa tgaacttccc tttatgatgg ttttaagtga
    29401 tgaaactgtt gctgatcaag cgggttttct gataaacgat caaggagttc agtataattt
    29461 tcttttcagc agccttgtac aagatatcca aggaacgatg agttttgaga ttgccaatca
    29521 attattcgaa taaggaggga tcagtgtatg gaataccacg aactaaatcc cttgattcgc
    29581 ggaagagagc ttgaactttt gtccaatgag acatttgaac ggatgattca aacaaattct
    29641 gtcgaagcac ttggtgaaat attgaaatcc acgatttaca gtccatatat ttatgacgga
    29701 tatgaatatg acttcgaaca gaatctttta aaggagcaga gccgtctatt tcgctggtta
    29761 aaggaatcag caccggagcc tgaaatcgtt tggatctata cgatgcgcta tactttccat
    29821 aatctgaagg ttttgacaaa agccgagatg acaaatcaaa atctggacca tttgtatatc
    29881 gacgatggat tttattcatt agaaaccttg aaggatgcca ttcatacgca agtttcaaat
    29941 gaactccctg aaagcattat ggacagtata cgagaagtac gtgattattt tgaagagtct
    30001 accattcttc agggaattga cgtgatttat gatcgacagt ttttgacaga gcagcgacgg
    30061 cttgccgata agctaggata tcaagaattg ctgacagaag tcattgcttt tattgacttg
    30121 accaatatca caacgatggc tcgaggaata ttgcagaaaa gaacatatgg gttcatgacg
    30181 acggtacttt ccagctcggg aagtatcgaa aaagaagtat ttttgaactt tgtagataaa
    30241 gatatggaag attacacgaa ttttctatta gagacagatt atgctgattt tctgcgccct
    30301 gtcatccgtg agggaaatct tgatctcgta aagcttgaac agttgaaaga tgattatcta
    30361 agttcattgt atcaagaagc acaaacccag gctttcggac cactgccact actagccttt
    30421 ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac tggttatcgg caaaagaagc
    30481 catttcgaac cagaaacaat cagaaagcgg gtgagacaag tctatgacgc ataagatcgg
    30541 tgtagttggc gacaaagatt ctgttttgcc attcaaattg tttggctttg atgttcgtta
    30601 tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga atgagtatgg
    30661 cgtgatttat gtcacagagc aatgtgcagc attgagccca gaaacgatcg aacgttacaa
    30721 ggaacaaatg atacctgcta tcgtattgat tcccaatcat caaggatcat tagggatagg
    30781 gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt caaaatatct tatagtatga
    30841 aaaggagata gaaattttgc aaattggaaa aatcataaaa gtctccggac ctcttgtaat
    30901 ggctgagaac atgtctgatg ccagtatcca agacatgtgt ctagtgggag atctaggagt
    30961 tatcggagaa attatcgaga tgcgtggcga tgtggcgtct attcaagtat atgaagagac
    31021 ttctggaatt ggtccaggcg aaccggttcg ctctactggt gaagctttat ctgttgaatt
    31081 aggaccaggg atcatttcac aaatgtttga cggaatccaa cggccattag atacctttat
    31141 ggaaatcaca caaagtaatt tcttagggcg cggagttcaa ttgcccgcat tggaccatga
    31201 gaaaaaatgg tggttcgaac caacagtaga agccggagaa acggtgagtg ctggagatgt
    31261 tatcgggatc gtagaagaaa caaaagttat aaaacataaa atcatggtgc caaatgggat
    31321 aaaaggtacg atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa cagtatgtgt
    31381 cattgaaacc gaaaatggtg acaaaaattt gacgatgctt caaaaatggc cagtaagacg
    31441 tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc atgatcactg gacagcgagt
    31501 gattgataca ttcttcccag tgacaaaagg aggagcagca gctgtacctg gtccttttgg
    31561 tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg acatggttgt
    31621 ctatgtggga tgtggcgagc gtggaaatga gatgacggat gttttaaatg aattccctga
    31681 attgattgac cctaatacag gagaatcgtt gatggaacgt acggtattga ttgctaatac
    31741 atcaaacatg ccagtagcag ctcgagaagc ttctatctat acgggaatca caattgcgga
    31801 atatttccgt gacatgggtt atgacgttgc tatcatggca gactccactt cacgatgggc
    31861 agaagcactt cgtgagatga gcggacgttt ggaagaaatg cctggtgatg aaggttatcc
    31921 agcttatctt ggttcacggc ttgcggaata ctatgaacga tctggaagag tcattgcatt
    31981 aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt gctgtatcgc cgtctggagg
    32041 ggatatttcg gaaccagtca cacaaaatac attgcgggtc gtaaaagtat tctggggtct
    32101 tgattccagt ttagcccaaa aaagacattt tccttcgatc gattggatac aaagctattc
    32161 tctttattca acagaagtag ggaagtatat ggatcatatc cttcaagctg actgggcatc
    32221 catggtcacc gagggcatgc gtattttgca ggaagaacag cagctgaatg aaatcgtgcg
    32281 tttggtaggg attgattccc tttcagataa tgaccgtctg acattagaag tggcaaaatc
    32341 cattcgtgaa gattatttgc agcaaaatgc ttttgatgat gtcgatacat tcacttcaag
    32401 agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag gacgccgtgc
    32461 gttgtcgtta ggtgcttatt taagtgagat catggacggt accgtatcga ttcgtgaaag
    32521 gatcggacga agcaaatata tcccggaaac agaattagaa aaactagatc aaattattgg
    32581 tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga ggcatgagca atgattaaag
    32641 aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa gttgctggtg
    32701 tcaaatatga agaattaatc gaagtccgta tgcaaaatgg cgagattcgt cgtggacaag
    32761 tattggaagt ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca agtggtatca
    32821 atttacgtga ctcttctgta cgatttttag gccatccttt agaactaggt gtatcggaag
    32881 acatgatcgg tcgagtattc gatggtttgg gacgaccgaa agataacgga ccagagatcc
    32941 tgcctgaaaa gcatttggat attaacggag aagtaatcaa tccagtagct cgggattatc
    33001 cagatgagtt tatccaaaca ggtatctctg caatcgatca tttgaataca ttagttcgtg
    33061 ggcaaaaatt accagtattt tctggctctg gtcttcctca taaagaatta gctgctcaga
    33121 tcgcgcgaca agcaacggta ctcgattcat cagatgattt cgccgtagtc tttgctgcta
    33181 ttggaatcac ttttgaagaa gcagaatttt tcatggaaga ttttcgtcaa acgggcgcaa
    33241 tcgaccgttc tgtcatgttt atgaatttag caaacgaccc tgcaatcgag cgtatcgcta
    33301 ctcctcgtat ggctttgact gctgcagaat atcttgctta tgaaaaggga atgcatgttt
    33361 tggttatcat gacagacatg accaactatg cagaggcatt gcgagagatt tctgcagcac
    33421 ggcgagaagt tccaggaaga agaggatatc ctgggtatct ttacacgaac ttagctactt
    33481 tattcgaacg agcaggaaga atccgcggac taaaaggatc ggttactcaa attccgattc
    33541 taacaatgcc agaagacgat aaaactcatc caatccctga cttgactggt tatattacag
    33601 aaggacagat catcttgact agagagcttt acaagagtgg gattcagcca ccaattgatg
    33661 tactcccgtc tctttcgcgg ttaaaagata agggaactgg acccggaaag acaagagaag
    33721 accatgcagc tacgatgaat cagctttttg cagcctatgc acaaggaaaa caggcaaaag
    33781 aattggccgt agttttaggt gaatctgctt tatctgatgt agacaagatc tatgcgaagt
    33841 ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac cgttcgatta
    33901 ctgaaacact tgatctaggg tgggaactgc tatcgatgtt gccaagaacc gaattaaaac
    33961 gaatcaaaga cgacatgctg gacaaatatc tgccagaagg gaagtgagtt ctgatggcac
    34021 gattgaatgt gaatccgacc cgcatggaat tgactcgatt gaaaaaacag ttgacaactg
    34081 ccacaagagg gcataaatta ttaaaagata agcaagatga actgatgcgt caattcatcc
    34141 tgttgattcg aaagaacaac cagctgcgtc aagaaatgga agagtctatg acagaagcaa
    34201 tgtctgattt tgtattggca aatgcttcga tcacggagcc tttcatcgaa gaattatttg
    34261 ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg agtgtaaaag
    34321 tacctgtgat gaattttgaa tatgatgaac agctgacaga cacacctttg gaatacgggt
    34381 atttgaattc cagtgccgaa ctggatcggt caatcgatcg gtttactacc ctgcttccaa
    34441 aacttttgga actgacagaa atcgaaaaaa cttgtcaact gatggcgagt gaaatcgaaa
    34501 agacacgtcg gcgagtcaat gcattagaat atatgacgat ccctcaatta gaagaaacga
    34561 tctattatat tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca aggttgatca
    34621 aagtaaaaaa tatgggtacc actgactaag tggtatcctt ttttaaaaga ggaaaacgat
    34681 gattaaacaa agcaaacaaa taaagaaacg cttgccggct gttcagcttc tcgcattagg
    34741 attttttctc ctgattttag ttggcggcgg cctacttacc ctaccatttt ttagtaatag
    34801 tgggcatggg acaaatttta tcgatgcgct tttcactgca acttctgctg tctgtgtgac
    34861 gggattgaca acattgaata cagcagagca ttggaacagt gctggtcaat ttttgatcat
    34921 gcttttgatt gagatcggcg gtcttggttt tatgatggtt ccgattctat gctttgcatt
    34981 gataaagaag aaagtcagtc tcagcacacg tattatatta aaagaagcgc tgaatctaga
    35041 agaagtatca ggtgtgatga agctcatgct ttacatcctg aaatttgcct ttgtgatcca
    35101 actgattggt acggttgctt tatcttttgt attcgtgcct gaatttggct ggttgaaagg
    35161 tatctggtac agtttgttcc atgcaatctc tagtttttgt aatgcagggt ttgatttgtt
    35221 aggagacagt ttagccaatc accagcaaaa tgtttatttg atcatggtgg tatctgcttt
    35281 gatcatttct ggtgggttag gcttcatcgt atggcgtgat ttactggaat atcatcagaa
    35341 aaagaagata acgattcatt caaaaatcgc attaaccgtt acaggtaccc ttttattagg
    35401 cggattcgtt gtgttctttt tcacagagcg caatggggaa acgctagtgg aagggaatat
    35461 gtttcaacga ttggcaaaca ccttttttat gagtgtgaca cctagaacgg ctggatatta
    35521 ttcagttgat tacctccata tgagccatgc aggtctgatt ttaacgatgt ttttgatgta
    35581 catcggtgga acatctgggt cgacggccgg cggattgaaa acaactacgt taggtgtgtt
    35641 gctgatccag atgcgttcga tgcttaaagg acgtacgaga gcggaagcat ttggccgtac
    35701 catccgacaa ggcgcagtgt tgcgggcact tactttattt tttgttacgt tgacgttgtg
    35761 tgtagtcgcc atcatgattt tatcggtgac tgaaacgata cctaaaactt ctggcatcga
    35821 atatattgct tttgaggtct tctctgcgtt tggtacagta ggtcttacga tgggattgac
    35881 gccagacttg actgcaatag ggaaattgat cattatagcg cttatgtaca ttggacgagt
    35941 tggtgtattg actgtggtct tctctctgat gacaaaagca caaagaacac aatcaaaata
    36001 caaatatcca gaagaaagtg tcatgattgg ataatttcat cttctgacag aaacctagtg
    36061 atttagtaaa ataagaaaat aaaataattt ttcttgcttc ttggatactt taagtgcaaa
    36121 atgttcgttt attttttgag gcactatccg ttgttgaacc tcgggtagtg cctctttctt
    36181 attgaaagga atcaataaga tttgattcct ttatttatca ataggttata attcatattt
    36241 ttttattttt tcttttacga tacttagtcc agaagctaaa atttcaaggt tattagagga
    36301 tagggaaatt cgaacgaatg catctggttg cgcacgtgta gtaaagcgtc tggaatgata
    36361 aagtcggata ccgttagcaa gaaagtcatt ctcaattatc ttaggatcaa tctcaggatc
    36421 aactggaaga attcgatagt aagggacacg attcgttggt ttttctactt tgaaaagaga
    36481 atcaaaaagc tgattggctc ttttcgtaag acttaattta gtttctaaga tcctatttgc
    36541 ttcatttgaa tggaggattt gagtgatgac ttccacatct aatccagaag ttttcacatt
    36601 tatattaaaa attgcttgtt ctatcttgtt ttggacatgt actggaacta caagataagc
    36661 aactcgcaat ccagaacaaa taaattttgt cattccagct aagtagattg tttggttagg
    36721 taataatttt tggaaaggag gaatgatcgt atgttgatag taagttgtta aaaatgaatg
    36781 gatatcatct tcaataaccc atagaccttc tttttcaatc acttcggcta attgctctcg
    36841 tcttttgtga gagatttgaa accctatagg attattacaa gaaggcataa gaaagacacc
    36901 atgaatcttc ttttttctac attcctgaat caatatatca ggtcgcattc cttgctcgtc
    36961 aaaatcaatt ggaacgatct ctaaatgata taactgagcc aattcaatga aattagcgta
    37021 tgtatatcgg tctgttgcta ttctatcacc tggagagaaa agtgcagaaa gtgtgatagc
    37081 caatccattt tgaacaccgg aaacaataga tagagattct aaggacggaa tgactccttg
    37141 agtttttaac caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg gatcttggta
    37201 attaagcaaa ccaacaagat ttgggttatt actagttgat tgaataaacg ggacaacgaa
    37261 tttattacat tcttcaaaag aactaaccag accaaaatca atgatatgtt cggttacttc
    37321 attggtagag atggtgatgg actcaaagac attgggagaa ataaatgtgc cacttccaat
    37381 attcgtatac agcaaacctt tctcaattcc atatttataa gccttgccaa cggtagtaaa
    37441 atttaaatct aaataatcag ctagctctct ttgggagggt aactgagtgt ttttctgtaa
    37501 tttattcttt aaaatatctt gttctaattg ttgaatcaac gatcgataat aaggacgctt
    37561 caatttttgt ttgtctggtc gccaagttaa cgggtaattg tcataagaat taataggcat
    37621 tttctacact ccttaaaatt gtataccata caattttacc attgattgta ttgattttag
    37681 aagttaatct tttgtgagga gggaattgtc tatgaaaatt attaattttg aggaagtagt
    37741 agcttctttt agttttgaag aagcgattca agtaatgaaa aaatgttttt ttgattatga
    37801 aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt ttacctgacg gaaaagacaa
    37861 aaatatgttt gcgatgatgc ctgcttattt gggggagaac agatattttg gaacaaaagt
    37921 cattaccgct tttccggata atcatcagta tcatttacct tctcatttag gagagatttt
    37981 cttgtttgat gctcaaaacg gactacccaa agcattgata aacgcaaatg cagtcacctg
    38041 gattcgaaca gctgctgtat ctgcgctggc aacagattac ttagcaaatc cgcatgcaga
    38101 aacacttgct ttaattggag ctggtcaaca agcattgtcg catttgactg cgatttctac
    38161 cattcgtccc atcactacag tgtttgttta cgatatttta gaagaaagac gaaatacttt
    38221 tatcgctaaa gctaaaaaac agtatcctaa aataacgttt ataaatgcaa tatcagtaga
    38281 agatgcagta cgtccagcgg aaatcatttg tacgttgacc tctagtaaag aggcatttct
    38341 tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct gtgggcacat ttactcctga
    38401 tacaagagag ataagtacat cgttgatgga aacttctcaa atctatgtag atgattactc
    38461 agctacctta acagaaagcg gggacctgct tatagccatg gcagaaggag atttagagga
    38521 atcttgtatt gttggtacat taggcgaatt agtttctgag aaaaaaacat tttataaagg
    38581 agaatcgagg ccgacgattt tcgacgcagt tggtctagct attgaagatc tttgttgtgc
    38641 agaatatatt tataacaaat tagagaaagg ggctttaaaa tgaacttaga ttattacgta
    38701 gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag cagcggttta tgtgctagaa
    38761 gaatggctgc cagaaggtac gatgcaaaaa attgccattg aaaacaattt atctgaaaca
    38821 gcatttacag tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc agatcgagaa
    38881 atcgatcttt gtggtcacgc gacattagca acagcatttg ttttatttaa ctattacaaa
    38941 atacctgatg aaacgattaa attttctact caaagcggta atctatttgt gacaagaatt
    39001 aaagattatt attacatgga ttttcctagt attatgccga aaaaagtgcc gatattgact
    39061 gaatatgaag aagcaattgg ggctaagatt aaagaagcct acttagcacg tgatttattt
    39121 ttcgtattag aagatgaaaa aacggtggct aaattgcagc cagattttac agctatcaaa
    39181 aattttgaat taggaataga gtcatcgtga cagcagaaag tgtgcaaaaa gactttgttt
    39241 ctagaacctt ctttccaaaa ttaacaatca atgaagatcc agtatgtgga tcagcacatt
    39301 ctaatcttat tccttattgg gcagaaagaa tgaataaaac aaaattgtca gcttatcaaa
    39361 tgtcatcaag gggcggggat ctagaatgtg aattaaaaga agatcgtgtg ataataggag
    39421 gaaaagcagt tttgttttct aaaggtacct cgtttatttg atttctttca aatgaattaa
    39481 aaataaattg gtggagagtg gcagccttat agctacaatg gttgttaaaa aaagacgcaa
    39541 actttttcaa tttaatgaaa agtttgcgtc tttttttaat tattggtgtt tgttttttat
    39601 tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga tgacttcgat tttataacca
    39661 tcagggtcaa tgacaaagta gtaagaaggc gtagtgccag gtaatccttt taagtcagta
    39721 atctcaagac ctgcagcttt atgttgctcg tgtaattttt ctaaatcatc agctgcaata
    39781 gcgatatgac catagccgtc tcctagatca tacgcaccat gatcatagtt gtaagtcaac
    39841 tctaattcat agtcatctcc aggaagtgtc aaataaacaa gtgtaaattt gttttctgga
    39901 aaatcacggc ggcggctttc ttcaaaacca aatgcctttt gataaaattc gattgatgct
    39961 tcaagatctt tcacccgaac acatgtgtga gccattttca taaattcatc tctccttttc
    40021 cttttactca atcataacaa aaagaagtaa gaaaatagtt gatttgtgct tgttcatgag
    40081 aaatttctca tgtaaaatgg atagaaaata atactgttaa ggtggaaaca attcatggat
    40141 aaaccaattt ttggtgaaaa aaagcaatct gctaattatc aaacccgtta tgcagcatat
    40201 attgtcatag aaagagacaa gcggattgcg gtgatcgagg ctccaaatgg tgcttttttc
    40261 cttcctggtg gggaaatcga aggagaagag acgaaagaag aagcgattgt ccgtgagctg
    40321 atagaagaaa tgggaattgt ggcagaaatt tcttcttatc tgggagaagc tgatgagtat
    40381 ttttactcga attatcgtgc aacttattat tataatccag gatatttctt tgttgctgat
    40441 tcttggaagc gtataggaga accaactgaa aagaccaata aaatttggtg ggtgagccct
    40501 caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga gagatggatc
    40561 gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt tctctcagtt tttttattta
    40621 ttacagtaca tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg atgaagtgat
    40681 gacatttgct gcttctttga caagagaaac ggcatttccc attgctactc ctagaccagc
    40741 atattcaatc atggataagt cgttctcgtt atctccgata gccatgattt catcttgatc
    40801 aataccaagg tgctgtgcga gattcgcaac tgccgcacct ttacttgctt ctttatttag
    40861 tacttcgaag taatagggcg cacttttgac cgttgtgtat ttatcacgga aagtttgcgg
    40921 taaacgcgca attgctgcat ctaaaatttc tggttcgtcg atcatcatca ttttgacgat
    40981 gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt agcggcatct tgactaaact
    41041 tgcttcgtgg atcgtataag cgctgatatc tcgattaggc gtgtagatcg ctttgtcatc
    41101 gatcgaatgg agatgactgc cgacacggcg tgccatcact tcgatctcta ggaaatcttc
    41161 atgagtcagt ccgtgacggg cgatgatttt acctgatttc gtttgttgta cgagtgcccc
    41221 gttataagta ataacgtaat cattatcttg aaaaaggttc aactcgttta actgttcctg
    41281 gacacctggt aagggacggc cagtgcataa aacgatcgaa acaccttttg caacagcagc
    41341 ggcaatcgct tcttttactt cttttgttac ttctcttttc tcatttaata aagtaccatc
    41401 gatgtcgata gcgactagtt tgatagacat aatttcctcc aatatatttt aatttatttc
    41461 aatcaatgcg ccattacgga tatggctggc aaattcttga taagtttcgt caaataaatc
    41521 ataatgatca cgcaaggagg gatcaaccat ttcttttgga aagaaaaatc gctcatctcc
    41581 ttgttcttgt cccgctaatg cagcaacaag ctgactaacc tgtgataatt ctatcaaagt
    41641 tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg ggcgataaaa
    41701 atcataaggc agatcgtagc tagaattcac agcagtgtaa taaacagggt tatagccaac
    41761 cttttctact aacaaagtga gttcttggac taattttgaa tcccggttgc cagaaaaagt
    41821 agcagatttc agtggtttac gattcaaaaa acgtttagct aaatcattta aaataggatc
    41881 agcagattca gaccattgag taaagtaagt gcccaagacc ccatcatcaa gtttgaggta
    41941 ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat ggagttcaaa
    42001 actgctggca tcttcttgat acaactcgtg tgcgcgatga agcagatgct ccaaaatcac
    42061 ttccatacca cgggaaacag gatggaagta cacttgcaca tacatttgat atcggctaac
    42121 gatataatct tccactgcat gcatgccact catggaaaaa gcgatcccgc cttcataagg
    42181 acgaatcaca cgtaatattc gggtaagatc aaatgttcca tattctgttc cagtgaaata
    42241 agcatcccga agcaaatagt ccattcgatc tgcatcgatc tgactagaaa tcatttggac
    42301 aacttgtggg ttggggtaag ttttttggat cacactagct accttttcag gaaaaccttc
    42361 ttctactcgg ttaagaatct gatacacttc tgtttctgga gaagtgatga tctctacagt
    42421 gatggcttca tgattcgtat ggaagatatg ttcaaatgta tgagagtaag gaccatgacc
    42481 gacatcatgc aacagtgccg cacataaagt caccagccgt tcctgatcat cccagcctcc
    42541 gttgcctatt ttttcttttg aaaaatttcg agaaaagata tcgcagattc gtcttgaaat
    42601 ctcataaacg cctaatgaat gggtaaaacg gctatgttct gctccgtgaa aagtgaaaga
    42661 agaagtgcct aattgtttaa tgcgtcgtaa acgttggact tctttcgaat tgatcaaatc
    42721 taagattact ttatgctgga catggatata gttatggact ggatcacgaa aaactttttc
    42781 cataggcagc atttgatatt tataaggggt tgtcataatg tagctccttt atgttattac
    42841 gttgttccat tctctggatt tggttgttcc attgctccag ctggatttct ttttttagcc
    42901 agtcggtaga atcctgtaaa tcacttgcag aatacagctt ttcaaaagct tttgcaaatg
    42961 cttctttgac ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt gttgccattg
    43021 tagcaggatc cactgcagga tagccatttg taccaaagtc ttcttttaaa gcagattgat
    43081 aaaatcgctt catgacttgc ccgcgttttt cctgattacc gttgacactt aagtacatca
    43141 taacagaaat cccatttttg atccgacgct gagcgatacc ggcaaatttt ttgccatgga
    43201 tgcttaaatc ataagttcca ggacaataag aatgggtgat ttcgaatgct tgtatcggtg
    43261 cctttggaaa agcgtattta gtcaatagga gcatttgctc gtaagcgtca tctatggtta
    43321 ttttttttgt gtcactttga gggaaaatca aagaaatatt taaaattcct tcatctgata
    43381 cgatccctaa tccacctgaa tttctgatca ctggagcgta acctgcatct ttcagtgttt
    43441 ggatcccgtc acttagaaaa ggagttcgaa catccttcat acctaagatg actgtgcagt
    43501 catattgcca gaaatgtaaa aagatttgct ggtgttcttt agaatattcc gttaatatat
    43561 ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat gtgctgatct agtcgaatcg
    43621 cagggttcat tcaaccggct cctttgtata aattcatatt ttctacaata ttatactcta
    43681 aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt tggttgacaa
    43741 aacaaaagaa actaggaaca atgtagctat gagaagtaaa ggagaatgaa taatggaatt
    43801 atcacaagga acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc aaggggaagt
    43861 gcaagatttt tattttgatt tgaaagggca aatggtgaca attggcgata cgctgtacat
    43921 acgttataaa gagattcagg aggagacggg cgatgagatt cctgtaacga tcaaattagt
    43981 tccagatggt catgtacagc tgatccgtgc aggagaaatg cggatgcgtt tgaaatttgg
    44041 ctataaagaa cggttagaaa catcctatcg aacaccatac ggtatgatcc agattgctac
    44101 ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc acagcaggga aggttcgaat
    44161 cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa tattatttga ccttggattt
    44221 tacagcgtaa atataaaaga aaagtttgat tttttgtgtc tgattttgta tgataaggag
    44281 tagactgtga aaggacgtgt cctgattgga aattagtgta tttgaaggtg caaacaaaag
    44341 cgaattatca atgatcgaag tagcacacgc gatcttagaa caacgtggcg atgtcatgga
    44401 tttttctgat ttagctaacc agatccaaaa ctatcttgag aaatcagaca gtgaaattcg
    44461 tgattcactc gcacaatttt atactgactt gaatattgac ggcagcttta tctcattagg
    44521 ggataatcgt tggggcttgc gttcatggta tgctatcgat tcgatcgatg aagaagtaaa
    44581 ccatggaatg gacgaagagg atgaagatac tccacgtcgc cgtaaacgca agaaagtcaa
    44641 cgcctttatc aatgatgatg aagacgcgat cgattacaat gacgatgacc cagaagatac
    44701 agatctgaca gaagaagatg acgatgatct gtttgatgac gatgatgatg aagatgaaga
    44761 aatcgctgct tataattctg acttgcaaga aatcggagca gatgacccga cagatgatga
    44821 agaagacttg ccaggaaata tcgaagaaga tttaagtatc atagacgaag acgatgatga
    44881 tgaagattat gaagaggaag aattttctga ctctgaaaaa gaataaacat tattttctta
    44941 cgagaggttg tgacagagac gatcagccta tcagaataag gaggtgtgac acgatttttg
    45001 tcacaccttc ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg
    45061 aaacttttag cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt
    45121 tttactggat ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc
    45181 atcgaaactg attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa
    45241 gaggccttag atgtgatgca agatagtttt cataaagcct tgaactattt tgaaaaagaa
    45301 aaagagatcg agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat
    45361 agttggcgca gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa
    45421 cagttacctt tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt
    45481 ccggaaaaag agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgca
    45541 actatcttgg atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct
    45601 ttacgtcaaa tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt
    45661 aaaaaaacta aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat
    45721 ccgccagcgt tttattatcg aagagcaatc atttataaaa gcaaggcgga gaaagagacg
    45781 tctccaaggg ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt
    45841 taatagtcaa gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt
    45901 gatcttaggc gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat
    45961 caatacacaa aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg
    46021 cgaaggacaa gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca
    46081 ttaccaagtt ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga
    46141 aaggcagatc acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga
    46201 caagaaaaac agtgtccagc tctcacttcc acttttattc aaagatgaca cttatctatc
    46261 tgttttgaca aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa
    46321 gtactattgg actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac
    46381 gcctacacga agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata
    46441 cgaaattgct cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa
    46501 aacgattttg gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca
    46561 gtttcaattt tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat
    46621 atagtgaaaa gaaattattt agtcatcagt attattgctt tatccgtgct tttgtttttc
    46681 gaaattactt cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag
    46741 cattcgtcac aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa
    46801 acagctaacg tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcaatcatca
    46861 caatcaacac aagacagtac agaagcaatc caagacccga ctgtcaaaag cgtcgcaatc
    46921 agctttgatg atggaccagg agcgaccaca acgccgcagc ttctgcgaat cttaaaagaa
    46981 aaaaacgtgc atgtcacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc
    47041 gtgaagcaga cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat
    47101 ctggccactc tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc
    47161 aaaaaaagtc actggcaaaa cccccgcttt tgttcgtccg ccctatggtt ctgtcacgaa
    47221 tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg
    47281 gaagacaagg aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatgggtc
    47341 aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga
    47401 ttatctgaaa gagcaaggct atcgcatcac gacagttagc gatttattag ggcatccaac
    47461 ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt
    47521 atctttaata aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa
    47581 ggcttaacaa t
//
LOCUS       DACEKQ010000010        42497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEKQ010000010 DACEKQ010000000
VERSION     DACEKQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.010"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..1087
                     /locus_tag="IW671_000511"
     CDS             5..1087
                     /locus_tag="IW671_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897524.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IW671_000512"
     CDS             complement(1305..2447)
                     /locus_tag="IW671_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897525.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IW671_000513"
     CDS             complement(2452..2637)
                     /locus_tag="IW671_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0897526.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IW671_000514"
     CDS             complement(2991..3236)
                     /locus_tag="IW671_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897527.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IW671_000515"
     CDS             complement(3230..3631)
                     /locus_tag="IW671_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0897528.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="IW671_000516"
     CDS             complement(3816..5624)
                     /gene="uidA"
                     /locus_tag="IW671_000516"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0897529.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5647..7413)
                     /locus_tag="IW671_000517"
     CDS             complement(5647..7413)
                     /locus_tag="IW671_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0897530.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7426..8220)
                     /locus_tag="IW671_000518"
     CDS             complement(7426..8220)
                     /locus_tag="IW671_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0897531.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(8230..9606)
                     /locus_tag="IW671_000519"
     CDS             complement(8230..9606)
                     /locus_tag="IW671_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0897532.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLVLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(9726..10373)
                     /gene="eda"
                     /locus_tag="IW671_000520"
     CDS             complement(9726..10373)
                     /gene="eda"
                     /locus_tag="IW671_000520"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0897533.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10466..11893)
                     /gene="uxaC"
                     /locus_tag="IW671_000521"
     CDS             complement(10466..11893)
                     /gene="uxaC"
                     /locus_tag="IW671_000521"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0897534.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11967..13046)
                     /gene="uxuA"
                     /locus_tag="IW671_000522"
     CDS             complement(11967..13046)
                     /gene="uxuA"
                     /locus_tag="IW671_000522"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0897535.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13049..14680)
                     /locus_tag="IW671_000523"
     CDS             complement(13049..14680)
                     /locus_tag="IW671_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0897536.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14986..15657
                     /locus_tag="IW671_000524"
     CDS             14986..15657
                     /locus_tag="IW671_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0897537.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKILEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(16360..16715)
                     /locus_tag="IW671_000525"
                     /pseudo
     CDS             complement(16360..16715)
                     /locus_tag="IW671_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16899..17306)
                     /locus_tag="IW671_000526"
     CDS             complement(16899..17306)
                     /locus_tag="IW671_000526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897538.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(17807..18001)
                     /locus_tag="IW671_000527"
     CDS             complement(17807..18001)
                     /locus_tag="IW671_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897539.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(18015..18353)
                     /locus_tag="IW671_000528"
     CDS             complement(18015..18353)
                     /locus_tag="IW671_000528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897540.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(18397..18729)
                     /locus_tag="IW671_000529"
     CDS             complement(18397..18729)
                     /locus_tag="IW671_000529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897541.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18743..18973)
                     /locus_tag="IW671_000530"
     CDS             complement(18743..18973)
                     /locus_tag="IW671_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897542.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKVYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(19195..19731)
                     /locus_tag="IW671_000531"
     CDS             complement(19195..19731)
                     /locus_tag="IW671_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897543.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(19752..20843)
                     /locus_tag="IW671_000532"
     CDS             complement(19752..20843)
                     /locus_tag="IW671_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0897544.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20851..23061)
                     /locus_tag="IW671_000533"
     CDS             complement(20851..23061)
                     /locus_tag="IW671_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897545.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(23077..25605)
                     /locus_tag="IW671_000534"
     CDS             complement(23077..25605)
                     /locus_tag="IW671_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0897546.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(25659..26069)
                     /locus_tag="IW671_000535"
     CDS             complement(25659..26069)
                     /locus_tag="IW671_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897547.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(26082..26300)
                     /locus_tag="IW671_000536"
     CDS             complement(26082..26300)
                     /locus_tag="IW671_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897548.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(26297..27289)
                     /locus_tag="IW671_000537"
     CDS             complement(26297..27289)
                     /locus_tag="IW671_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897549.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(27368..28279)
                     /locus_tag="IW671_000538"
     CDS             complement(27368..28279)
                     /locus_tag="IW671_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0897550.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(28298..28642)
                     /locus_tag="IW671_000539"
     CDS             complement(28298..28642)
                     /locus_tag="IW671_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897551.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28662..29219)
                     /locus_tag="IW671_000540"
     CDS             complement(28662..29219)
                     /locus_tag="IW671_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897552.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(29299..29799)
                     /locus_tag="IW671_000541"
     CDS             complement(29299..29799)
                     /locus_tag="IW671_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897553.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(29800..30345)
                     /locus_tag="IW671_000542"
     CDS             complement(29800..30345)
                     /locus_tag="IW671_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897554.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(30342..31598)
                     /locus_tag="IW671_000543"
     CDS             complement(30342..31598)
                     /locus_tag="IW671_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0897555.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(31961..32275)
                     /locus_tag="IW671_000544"
     CDS             complement(31961..32275)
                     /locus_tag="IW671_000544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897556.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(32402..33595)
                     /locus_tag="IW671_000545"
                     /pseudo
     CDS             complement(32402..33595)
                     /locus_tag="IW671_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(33785..34141)
                     /locus_tag="IW671_000546"
     CDS             complement(33785..34141)
                     /locus_tag="IW671_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897557.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(34207..34659)
                     /locus_tag="IW671_000547"
     CDS             complement(34207..34659)
                     /locus_tag="IW671_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897558.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(34661..35026)
                     /locus_tag="IW671_000548"
     CDS             complement(34661..35026)
                     /locus_tag="IW671_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897559.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(35043..35237)
                     /locus_tag="IW671_000549"
     CDS             complement(35043..35237)
                     /locus_tag="IW671_000549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897560.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            complement(35254..35499)
                     /locus_tag="IW671_000550"
     CDS             complement(35254..35499)
                     /locus_tag="IW671_000550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897561.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            complement(36215..40510)
                     /locus_tag="IW671_000551"
     CDS             complement(36215..40510)
                     /locus_tag="IW671_000551"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0897562.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(40657..40896)
                     /locus_tag="IW671_000552"
     CDS             complement(40657..40896)
                     /locus_tag="IW671_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897563.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(40986..41258)
                     /locus_tag="IW671_000553"
     CDS             complement(40986..41258)
                     /locus_tag="IW671_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897564.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(41341..41592)
                     /locus_tag="IW671_000554"
     CDS             complement(41341..41592)
                     /locus_tag="IW671_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897565.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT        13141 a         8763 c         5956 g        14637 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaaaa
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctaatttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gacaaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaaggag atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taactatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tggatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcatttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagagag cccctttttt tcgaaaaaac tagaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaacgat taggctaggc
     3781 taatcgtttt ttgaacattc ttaaaaaaat ccaaattatt ttttataact gaaattgggt
     3841 attgatgacc atcgtttctg tagatagtga gcaatcattt taggttttct atctctagta
     3901 aagattcctt tcttatttcc ttgcactctt aaaatccctt gactcgttgc aaaatctgca
     3961 aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc gtgatttgct
     4021 tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc atgaaatcca
     4081 gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca tctttctttc
     4141 catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc tcccgaaaca
     4201 taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac cttacaaata
     4261 tctggcgttg attttagata agtaactatt gttacaggtc ttttctgcgg atcaagttct
     4321 ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc cgaatctggt
     4381 tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc tatcaattcc
     4441 ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt atgtttttcc
     4501 acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc aggtacttca
     4561 tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga gtaaggataa
     4621 tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc tttaatattt
     4681 acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc aaatccttta
     4741 aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac actgcgaata
     4801 ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact aattttcata
     4861 ctatataaat aagcatctag aggttcccaa aaaattggat tcttaagctg aatcattcct
     4921 ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc tattaagatt
     4981 tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc agttattctt
     5041 gtattaatag taatatcact tatatagtct ttcggagttg tatataactt aacaggtcta
     5101 tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt tatatcccca
     5161 ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc aataatattg
     5221 tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat ttcagactca
     5281 aatggtgtaa acccaccttt gtgttgagta atcaactttc cattcaaaaa aacttttgct
     5341 tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct tttaggaaga
     5401 gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc tgtttcaaat
     5461 aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt tattggttct
     5521 aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca gagaccgctt
     5581 aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac cactcctatc
     5641 taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac ttactccctt
     5701 aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt gttttccatc
     5761 ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg gacactgtac
     5821 agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag ttcctttact
     5881 tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat taatgatcaa
     5941 gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc cagctatatt
     6001 tttaattttt tcttcttgcg ataatccttt caactcttct tcaggtgaaa tgtatttttc
     6061 aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt gaggtctatt
     6121 tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta gtaaaatcct
     6181 tttatatttt tctggttcaa taggccaaat atcttgttta tctttaacta atgtaattgc
     6241 ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt tttccagagc
     6301 ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca atttagcttt
     6361 taaccctaaa attcttttta aggcatctaa taaccgatca tcagagataa ttcctttacg
     6421 ataaccttcc atcatataat taaagtcttc atctggatca ttaaaaaata gaaatagatc
     6481 acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc cagtcattcc
     6541 taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc ctctaagtaa
     6601 gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt tcagatcagg
     6661 attaaaatgt tttgaataag ctggcatatg gatatgacct gccataatcg aaggtaatcc
     6721 agcttctatt aaacctgagt atacttttcc aaaagaatta tcccattctt ccgtacctaa
     6781 actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac cagggaaatg
     6841 tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta aactaagttc
     6901 taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat ttctccaatt
     6961 tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg atgcttcaat
     7021 cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac caatttttac
     7081 ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt ccgtattagc
     7141 agcaattaat aaaggaattt tgctttctgt ttgtaggata tagttttgat catatacctc
     7201 atcagcagtt cctgggttat atcgtacagc tccaatatgg tactgattga ccatattact
     7261 caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta ttttttcttc
     7321 caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat ctaaattata
     7381 tggttttttt cgtaagtcta cttttatagt cattttcttt ctcctctact tattagtaag
     7441 atttttccaa aaagtatcta taaatttttg ttcaatatct acatactctc ccgataaaca
     7501 tttaaataaa acatcagttc ttaagttttc ccatgattct ttttctttat taaccaaaat
     7561 aggataaaag aaaactttat ttttatttgc agcttctaga tcaccaggag aatcaccaat
     7621 cattaatatc ttatccaaat cacaaccatt ttcaattaca cttcgaataa tgtcttcttt
     7681 ttttccttta tcttgacaac acagttcatc aactaatgat aataattcat gtctttccca
     7741 ttcttcttga acagcttcct tattagctga gcttacaata attatcttcc catatttttt
     7801 cagagctttt atagtatctc tagcacttaa aaagggttta tctaatcctt tagcttgact
     7861 aatctgttga ttgacttctt ccgaccattt taaagctttt aataaatcct ctgactgata
     7921 atattctatt tccctcttca aagatggatt agacaattct tttgttagag atacccagtg
     7981 ctttaatcga ttaatggatg ttacatcatt tttttctaat gttttaacta aacctaaaaa
     8041 tctgtttatt ccacgagtct ctgaatataa attaatatta ttccaatcct gtaggaattt
     8101 ttctctatga tgaacatcaa aaatctctgc agcaattggt ccgaaaaaga gattgtgttt
     8161 ataagtcatc gtatccataa cacagccatc tgaatcaata caaaatatcc aattattcat
     8221 atttctctcc tatttatatt tttgttttaa aagctcgcaa ttttctaaga ctttatcttt
     8281 agataatgga taaaggaaaa ataaaattaa tcctacaata aaaaaacaaa ttgatggaat
     8341 taatgttgaa gcgttatata acttttctaa aacatctccc gtttgtatat ttgctaaaga
     8401 gtcatagcct ataatagaaa gagtccaacc tccgacgcct ccagctagag cttgacctat
     8461 ttttctagca aaagaatata ccgcatatac ggtcccatct tctctttgat cagactttac
     8521 ttcttgatca tcaataacat cagtaatatt tgcccaaata acagtattaa acacacccaa
     8581 acccatatag ccaaccgaac taagaattaa gaatacatac atattactag tttttatcaa
     8641 aaataagatc aaatatacta ttgctgaaaa gaacattcct actgatgcca gttctttttt
     8701 tccatatttt ttacttaaaa acaaagttaa aggtgaaaca actattaaaa ccaaaatagg
     8761 attgataaaa ttcattaaaa ctattccctg tgcatcatta tagtaatcag gaaatacata
     8821 attattcata ctcataatta ataattgagc aaggactaag aaaatagccg caccaattat
     8881 agaaagtaat gccctatttt taactaaccc ttttaaagat tttaacaatg aaactttgaa
     8941 ttgagattgg tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat
     9001 aagtgcacat atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat
     9061 ttgagcgcct gaatcatctc ttgtataaat aaataatggt gttaatacac caatgatcaa
     9121 acttgctaaa gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat
     9181 agaagtaata gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact
     9241 cccccataat atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat
     9301 catagaagtt tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat
     9361 ccacggttta aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt
     9421 tacatcagta actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc
     9481 gcttatcccc aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt
     9541 aaaatcgttt ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct
     9601 ttccatacaa ttctctccct tcttttgtta agcgttttca ttcttatgaa agtacgtact
     9661 atactctgaa gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt
     9721 ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc tactcgactt
     9781 acttcttcaa aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca
     9841 acaactccta cttcaaacca atcttctaag ttctctagac ttactccgcc agtaggcatt
     9901 atatttacgt gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa
     9961 cctgggaata gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt
    10021 gtcatacaac ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga
    10081 tcaaaagatg gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac
    10141 gcatctaaaa ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca
    10201 atttctttga ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt
    10261 ccacccgcaa taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca
    10321 accgcaatca ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc
    10381 tcctctatct ccttctttta tggatacata ctttgtctag ctgtactaac ttaaaaagaa
    10441 taaatttaag cttttatttc aagtattatt ttctacttcc gatttcaaaa aaattaaaat
    10501 aattatacgc attacgataa caaatattct ccactatctg actaagatac atttcatcat
    10561 ctggaattcg gcctgcttta acccactcgc ttagtaattg acataatact cgacgaaaat
    10621 actcatggcg aggatagctt agaaaacttc gagaatcagt caacatacct gtaaaattcg
    10681 acaacaaact ttgttgcgcc atcgtagtca actgttgacg cattccttca gctgtatcat
    10741 tgaaccacca agcacatccc aattggattt tttgaatcat acccccttgg aagctttgca
    10801 ttgctgttgc tagtggaagc caatcagttg gatttaatga atataaaatt gtttttggta
    10861 agcacccact cttttcagcg gtatcgaaca atgctacaac attttgtgtc aaactagctt
    10921 gatcccccat cgaatcacca ccgacatcta ctccaatttg ttcctttaat atacttgaat
    10981 tatttcttaa agcatttaaa tgcatttgca tcgtccaacc atatcttgta tactcagaca
    11041 tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc tgataaatgg aaatccgttt
    11101 ttcctgctaa agcttttatt aacagattgt cgagtatttt ttcatctgct atttcataaa
    11161 aataagtatt caaaccgtga tctgctaatc gacagccaat ttgatgaaaa tgatccactc
    11221 gttggctcaa tgcttgcttc aacccattaa agtctttgat tggtatagcc gctgcttgac
    11281 taagttttaa aatatagttc gaaaagcctg atgatttgat actccagacc ttatctggac
    11341 ggaaagtagg taataccttg aaagaattat cctgtttaga aagctcagta tgatacccca
    11401 agtcatctgt aggatcatcc gttgtacaga tggctttcac atccatcatc tccaacaatt
    11461 ctttcgggcg ataggaatcc gtttggattt tttcgttagc acgtcgccaa atttctggtg
    11521 cattcttctc agaaattgtc aaatcaatat caaagtaacg ttgtaattcc aaatgagacc
    11581 attcaaaaat aggattacct ggcgccttct ccactgtttt tacaaaagct aaaaattttt
    11641 cataatcatc acctggacca ctgatcaact cttcttctat accattagct cgcatcaatc
    11701 gccatttata atgatctcca gctcctttat catttaacca tacctgagtc aaattcttgt
    11761 attgcttatt ttctagcaaa tatgctgggt ctaaatgaca atgatagtcg ataattggtt
    11821 ggtgttttgc aaatttatga aaaagtcttt tagctatttc attctctaat aaaaagttct
    11881 tatctaaaaa catatttaaa cactcctttc cttccgttag tttttagaag agaaaatttt
    11941 tcctctaaat aaactccatt atataattat ttactcgccc ccttttcaag cgcttcccaa
    12001 ataccattta aataaactgc acctagagct cggtcatata atccataacc aggtctaccg
    12061 gtttctcccc aaatcatccg accatgatct ggacgaatat agccattaaa tccattatcg
    12121 tacattgttt ttaatatctt atacatatct agagaaccat attctgattt atgcggcgct
    12181 tcgtaaaaat cgagtccatt gtcacttaaa tatttgatat tacgaacatg tgcaaaagga
    12241 atccgatccc gttgacaaaa ttcagataga atttcataga catcattatc tggtcgttct
    12301 gcaatacttc cagtacataa tgtaatacca ttagaaggag aatccactac attacacaac
    12361 caatctaaat cgtcacgatt tttaacaata cgaggtaatc cgaaaatgga atatggtgga
    12421 tcatctgggt gaactgccat tttaatccca acttcttcac atgtcgggat aattgctttg
    12481 agaaaatatg caaagttctc tcgtaatttc tcttcatcaa cttctttata ggcttctaat
    12541 aattgcttaa tttgtgataa ccgatgaggt tcccaacccg gcaattcaaa attattagta
    12601 gaattttcta cttttctaat aatttcctcc ggatcagtag ggatattttt tgaaatatag
    12661 gctaacgtag aagacccatc atctaaacga taatccaaat ccgattttac ccaatcaaat
    12721 actggcataa aattataaca gataacctca atcccaaatt ctgccaaatt gcgaatcgtc
    12781 tgtttataat tctcaatgta ttgatctcgt gtttgtaaac caattttgat atcatcatga
    12841 acattaacac tctcaataac tttcaaggta agtccattgg cttcgacttc atcttttaaa
    12901 gatttaattc gttctctcgg ccaaacctct ccggctggta tatccattaa tgttccaaca
    12961 attcctttca ttgctgggat ctgacgaatt tcttgtagtg agattttttc ttcatttgcc
    13021 ccataccatc taaaagtcat ttccatagtt actttcctcc aacttctgtt atatatttat
    13081 tgattgtttt acgaactgct tgaggaccta ctagcatttc gcaaaagatt ttttcagttt
    13141 tctttcctac cccaacttga tataaatcta aaccaaaaat ttctttattt tctaaaattt
    13201 cctgtaccgc acaatgaata tttgaaggat cttgttcacc taatttcaat actgataatt
    13261 tatcctgcaa ctctaccaat aacggatcag gacttggcac aaaattttcc ccttgatcat
    13321 cgactgccag taaataacgt aaccaagcag caatagcgat aggaataaat gttagatttt
    13381 ctgcatttcc gtctttttcg acatactttt taattgtttc accatagcga atcgctactt
    13441 tttgagaagt atctgttgca attcgttgtg gtgtatctgg aatataggga ttaggcaaac
    13501 gtttattaat tacttcatca ataaattgtt ttggatcaat aatgcctgga ttttcaacta
    13561 ctggtaaacc ttctaaataa cctattcctt tgattaaacc aaccaaatca tcatcctgca
    13621 tctctgctga aattgattga tatcctagca gacaacctgt tactgccaaa gcagtatgaa
    13681 gtggattcaa gcttgtcgta accttcatga tgtctgcttt atcaaccgtc tctctatctg
    13741 tcataatcac accagccttt tccaatttag gccggccatt tggaaaacta tcttcaataa
    13801 ctaaataatg aactacctca gtattagtga aagcagcaat attggtatgt ttttttgtgt
    13861 gaatgatatc taaatcctcg agccccatca tcaatagatc ttcctttaca ttctctgaag
    13921 gatttggcgt aattcgatca atcatactcc aaggaaaagt cactttttct ttatccttta
    13981 aataagcaat aaatttttca gaaacatatc cttctttttg ccaagcttct gcaatcgtaa
    14041 aaatactatc ttgaaaacgt tgaccattct gtgagaaatt atcagtagaa accatcgcaa
    14101 tcggcaactc tccagcatta aatctgctat ataacaagga tgttacaata ctcatcgtat
    14161 gttgaggatt gctaggtcca gatttgatat cagctttgac taccgaaaag aattgccctt
    14221 tagaatcttt taaactgtaa cctttttcag taatagtaaa cgttgtaaac tgtaatttag
    14281 gatcttcaaa atatttaacc actctttgat agtccgctat attcttcgga ttgcaatata
    14341 aactatcagc cgttactgcc aaaagccttt ttttgagttc accatctcca ctcataacaa
    14401 tttccaaaat gtcattgttg tagtcatgat aagctttttc cactacttct tcatcaaatg
    14461 tttcacacac aactactcct gtttctaatt cattttgatc tgctaagcat tgagcaattt
    14521 ctgcatgaaa tcctcgataa agatttcctc caccaaaatg aagccatact ggatgttgct
    14581 ggccttcttg cttaacttct ttgacatcaa aacacggcag tagaatctgc atttcattga
    14641 attcttcgat agaattttgc caatttttaa tcgaaaccat aattaacctc cttaaactaa
    14701 tatgttagtt ttgatacatt ttatatttta aaacccgttg taaccgttgt caatataaaa
    14761 atggaataaa cttgcttttt ttctaccata tcagaattat tgtatttttt attcagaaaa
    14821 ataaaatact ccttcttttt ggactgattt tatcgaaact tatttttttc tttttttcat
    14881 gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa
    14941 cagaattaaa atatcagcta aaataaaagt taaggagcat tgagtatgac aattaaaact
    15001 agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa
    15061 cctggaaaaa aagtatcaga aaaaatatta gaagaaacac tttctattgg tcgtacacca
    15121 atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc
    15181 ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa
    15241 catttggaac gacaaattat gttagagtgt tgtgccaaaa ttaataacca aaaaaaacgg
    15301 attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt
    15361 ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa
    15421 gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgttg gctacgagta
    15481 aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca
    15541 ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg
    15601 cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaag tatataactt
    15661 ttaactcttt ttaaattaaa tgattgttct gatcagaaaa atatcgcttg ttttcttatt
    15721 atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg
    15781 ataactaatt atgactgctg ataacttttg atattaaact agtatctcca atccaaataa
    15841 aaaatagata tcttgttcat caaaattagt tcatggatac ttaccttcat ttacatcatt
    15901 cagcggtttc tcattttctt ttattctatt atttcatttt ctaatagact tggcttcatt
    15961 tcgcaatact tttttacaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt
    16021 ttgtgacatg ataagtatgc tcctttttgt cttttccact aatacaacga tcaaagggtg
    16081 cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg
    16141 tttcaactac atcttatatt tcataataga ataaatagcc ttttgcatag ctgcttaact
    16201 tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat
    16261 tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta
    16321 ggattgatgc cgattactta tattctacgt atagttttag tattcttttt ttatcaccag
    16381 taaccaattg agaacagtcc taaagcagaa tagtctttta tctcaaaacg cttaatttca
    16441 ggcttaggaa tatgaataat cttttgagta tattctttct cttttaatct tactgaagaa
    16501 ataccactgc ctatcttttt tacaaatatt tcatttggtg tacagactaa taaaacaata
    16561 tttgtaatca atccttgcac taaagaatta aaaggaagac tatttaaaac agggtgtgca
    16621 taactataac atgtgaagta gttattttga actttaactt ttaaaaactt taaaaataat
    16681 cttatttgtt cttctaaaca atcatctata ctcatataat cactccacta attacttccc
    16741 ccaacaagaa atagcattaa tagctatacc agctgcaata gttgcagcat tgaattttgc
    16801 aaattgtgca ggagttaaac ttctttttgc tacatctaaa agtattttaa cagcataatc
    16861 ataatttttc ccttgcaacg cattaattaa agtttgcatt agattttcac gattaagtat
    16921 atcccaaaca acacaccaaa tggtaatgag ttcataacaa cacatgcagc atacttttga
    16981 agatcacgtt tttgacgctt tcctgttggc tgcacaaaat tttctaaaat aaatttccct
    17041 gctatacctt cagagccatc taattcagct tttttctgta tagcttctaa atcagtaact
    17101 atatattgat gtgtttctgt atcaacataa ccagcttctt ctaaatagaa tttcaaactc
    17161 tctactgcct gttgtctttg tgtttctata tcaggaactt cattcacttt ttcagcagca
    17221 taaacactgg ttccggcacc gactaacgta cttaatccaa ttaacattac acctaataat
    17281 gaaaaaaact taaaaaaacg actcattttt atccctcctg aaaagtattg ttgattatca
    17341 atttttatcc ttttaagctg ttcaataaga cgtttttacg ttccttccgt ttctatttta
    17401 ttctttctgt cttataacag cttttttcaa gtaaagaacg atataattct tcttaaaaaa
    17461 cagtctattt tttcggtata taataaaatt gtattgttac ctacatagcg aaacagtacc
    17521 aacatcattc aaatgaagat agcaaatttg cttcttcctt tttcttaagt gactatttaa
    17581 ataaccagaa aaaaactaaa atcacactga tatcccaaat aaattgaaat aaaggaaaaa
    17641 gtttttttct accatgatag tttttcaaaa aatagaaact cgtactattt aaaagtaaaa
    17701 taactagcat cgtaaagact actgatttat ctaaacttct ataaaaaagt gggctaaata
    17761 ataaagtaat taaaaaatga actatttgat attgtttttt ttcatattaa cagatcgcag
    17821 ctcctgcgat tccataggct gttcctacag caaatggaat aacggctgca cctactcccg
    17881 taaggcttaa acctagagca gcagctgtat aaatcccaga atgtagaagt ccagccccag
    17941 tgaaagccca actgcaggca cttcgttcac cagttttatt atttgttttt tgatctttca
    18001 tataattctc aacttcatga agctgatcgg ttagccatcc atggccattt tttttgaata
    18061 gaacgattaa attcatttag atactgttgg atttcagaaa ctgtaatttc agaattaatt
    18121 ctatctaaac ttgattttaa atcactctca tcaataatat atactaatcg atttccttta
    18181 gaatctttat gtgtggctaa attattttga atgatattta tttcagtttc ttgttgtttt
    18241 actaaaaaat tatttgtttc agaagcatcg acacttattc cagcgctaac aaaagtactt
    18301 aaacctatta agaaaacacc taaaatacca aatattttaa ctattctttt cataattttt
    18361 cctacttaaa aataagttat ttttaaaata tcttgattat gaattcatcc cccaaaggcc
    18421 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcatata agtcttttaa
    18481 atgatcccat accagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18541 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18601 atcttggatt tcaataatag tatcatcaat tagtttttga atttctttcc tatcaacata
    18661 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18721 ttcagacatt ctcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18781 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18841 gatttgtcgg tccatacgga tagacttttt gacattcaat gttcggatta taccagatag
    18901 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18961 aaccaataca catcacacta tagccaatta attttttcat ctcgtatcca tctcctaaaa
    19021 aatagaatag ttcttgtagt tattcataag acatattttt tccgtttttg ttttatcctt
    19081 tctgtcctat aacaactttt ttcaagtaaa gaatgacata cttcttctta aaaaaacagt
    19141 ctgttttttt ctattttttc ggtgcataat aaatatgagt gttcattttt tattttaata
    19201 cacacttcgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    19261 caattgaagt aatacaaatt gagtgacctc attcgttgtt tcttctccaa ataacaaata
    19321 ttgatccgga ttctctatat cttgtgtcac ctggtgaagt ttcccagttg ttttcatttg
    19381 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatccgtca tatagtccct
    19441 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    19501 taagaaattt tttgtcattt catctatttg ttgcgctgtc tcgtcttcta atgaaccaat
    19561 ttgactgtta ctttcagtac tttgaagagt tccacttgtt tcttttactg cttcgattgt
    19621 ttcttctgtc gttgtcaatt gtgttttttg tttttcagaa gaaaaccaat gacaaccagt
    19681 aagacctatc aaacataacc aactaaatac taaaattatt ccttttttca cgcttctaga
    19741 cactgccttt cttatttcac cttacgaacc agactacgaa tttttgtttg attgtacgtg
    19801 cgttgatacc gagccataat ccgtccttgc tcggcattct gttccacact agagaaatgt
    19861 tgtccgccat ctgaaacatc tgttataatt ccggtgtgac catagattcc aggagacaat
    19921 gcaccacctg cttgccaatt caccacatct cctgcttgta aatcacttgg ttttgggttc
    19981 atgatcactt gccaaccata ggctgtccaa tcataatctt cccctattaa catcgcaaac
    20041 atgtgtccag aacccattaa ctgtgggcca cctagttggg atacatacca cgccgtgcca
    20101 ccataacact ctccattatt taccgatcgt ccaatgatcc gatctaaaac tttcgcatta
    20161 cctttacttt tccctgcaac aagtgacgat ttttttgttg gtaggctatt ccaatgtttg
    20221 atcgcaaaag tatcatattc tgttaagtta taagacttta tgatactcat cgctccttgg
    20281 taatacggac tgccttcggt aatacctgcc gccgcatagc ctccacgacc tagaatcaat
    20341 aaacaactcc caggatcttt attgcccaca cattgattaa agttgctttg actcgccacc
    20401 aaaaagccgt aatagtcata acaatctttc aggttcggaa acttcatata gaagccacct
    20461 tcagaaccac caatcccccg tgcagaacct tgtggatagg gacaaccagg aaaccaggta
    20521 atcccaaaga agtttgtatc gtttcttcca gattgtgatt gtccataagt cgattcataa
    20581 cctaattgag aaagaatcac acttggtaat aactgatact ttaccgctgc accgactgct
    20641 gcttgcgcat gttcatctgt gatcggacca cgtcctttaa tctctgaaaa aatcggtaat
    20701 ccttcggtat attcccaatt aaaagcgaca gctagacctg tagcctctcc tccatcctcg
    20761 gaaagttctc ctgaaaaaaa gaatggcaat agaaaaaagg gaagaataaa gaaaagcaga
    20821 caacctagac accctttctt catttcttcc tcatcctttc ttcgataatg gtttttgaac
    20881 tgttcgtcct tttggtaaag taaaatcggt tcctggtttt gcgtccaaaa tcggcaatgt
    20941 ttcatttttc ttttctattt ttttcgattg ctctactgga ttattaaaag aagaaaatgc
    21001 aggttgtaag gattttggta cggaaatcga cggtttactt tcagacgaac gttgtatctg
    21061 tcgttgcaca gaacgatttc ctgatagagt ggctaccgac gacttaggaa cagagtattg
    21121 attcaataac tgctgcattg ctggataact cccatttttc gcctgatttt tcacttggtg
    21181 aatctgttgt gaaagagaac cagcagctgt taattgtggc acgggtactt ttccagcagg
    21241 caacggaatc gattgaccaa caaaagcgat acctttattt aacaatggtt ttctttgatt
    21301 attcccttgt tgcttactcg attgtgccaa cgattgtacc tgttgtttcg gtcttactga
    21361 attcatggac gaatttggaa tcaaaccatt tgaaagacca gaattccact ctgtttttga
    21421 tttggtagaa gacatttcag gctctacccc atgataacga acatgacgac cactataagg
    21481 caagtcttga tttgaacgcc cacctagttc aaagggatta atcacttgcc ccccttttac
    21541 cgattcttgt ggtgtacgtt tttgttgaaa cttttgaaat ccatgtatcg ctgcagaagc
    21601 gagtggattc gttgctcggt acacattccc tgctaatcct gcacctttga ttgccaaatt
    21661 ttgcccagca gttgatagtt gaccaggcag ttgtttcatt tgagacaaag aatgacttgc
    21721 tatgtttttc gtgttattta tggtttgatc tgcagctcct aaccctagtt tctttaataa
    21781 atctttccgt tttacaatga gtaaaattga taggagtgct ttgacaaaca tatttcctaa
    21841 aaaaccaaaa acagtattca ttagaattaa atcgtctact atgttataga tcaaaaatgc
    21901 caccaaaata atcacactgt aaaaagattt ttgaaacatc aagctcaaaa aggactttaa
    21961 ggtattcatg atcatataat caaatgctgg aacaaaagaa gcgagtaatg aaaaaggaat
    22021 cacaagtaat aaacacaaca ccaacatttg aaagataaaa cgaatcatgc caatcactac
    22081 aagtggtaat ccaatactta gattaagcaa tggcaccata gaaccaacga ctgctttata
    22141 aacagcttta gttggttgaa tatagacacg ataagccttc tttttccctc tatcttctcc
    22201 tgcagcagtg gtcacttttg tctcaatatc agataattct tcacttgacg cttccttggc
    22261 tagaaattct tgtggttcaa tctctgcttg atacaattct tctaaatttg ttgtcccata
    22321 atttaacaat agatacggat caacaacagc cgctttataa taaagttggc gcactttttg
    22381 aatggcttcc ttttgtgtcg ttggcactaa aacaccattg acatcttttg tcgccaattc
    22441 atctagacta tttgtagcag aaaaaataaa gccttctacc tccacggaca aatcatttaa
    22501 ccctttagta aaagcttctc ctttactagg tacgttacta ccatagccaa accaaaccat
    22561 tgaaaaaatg acaacaaaga gaaatcgaat aaacaccatt tttgcgtaat gtttccctct
    22621 aaatatacga agagtaaata gatataacac atacaagacg acaatccccg caccaatagg
    22681 atcgtatatt ttcgaataaa tagtatccga aaaagtgaaa aactggtgaa ttaactcgtt
    22741 cattgcagct ccttcataga gcttctcaat gacaaaatca aggccttgcc aaatcaattt
    22801 acttaaacta aagaacatat tcgtcatcat ttttagaaaa tctgtgccat ttgctccaaa
    22861 taacattttg cctaatgaaa attcactatc agataaataa gaagaaaagg tatcaatatg
    22921 aaatttcccg atctcataga tcgattgatc cacctgtttt tctaccgcat ataccaatcc
    22981 accaacaaaa aacagtccga atccaaccgt taaaaaaagc caaacgcacc atttcttttc
    23041 cttacgcttt atataagcca ttgtttcacc tcgtttctat tgcatgatta acgtatcctg
    23101 ttcttctatt tgagagtgac ttgttttttc cgttgtttgt aatgcagcgg tcatttcttc
    23161 aaacaaactg tgtacattga gttttcctac acgtccatag ggatctaaca tccaacactg
    23221 tcctttaatc atatggctca tttcagataa gttcgcttca tttacttcca aacgtaaatg
    23281 agtcaaaatc ttttctcgct cactgtcttc atcaaaagcg aagacaacac caaaattccc
    23341 tttttcttta tctgaaccta agtcgccaat aaattgtgta tctagcacta attgattcat
    23401 ctgtgaacgc cccacacgcg ccattgctgc aataatacta cgtcccgcgt tcgaattatt
    23461 aaatacccat gcttctgtaa aataaatcgc tgttttctta ctactatctc gttttccaaa
    23521 catttcacaa aaacgaccta aactaatcat caaacacaaa gactttcgat ccgcatctgt
    23581 atatagttct ggtcggacat tgtcatttgg taatttcaaa tcttggatct ctaaaatcgt
    23641 tgttttggca ttaaagtcta atcccgggtt ttctccataa gaaaatccta aacgtaaaat
    23701 cgaatcttct accgtcaagc gtaaaaactc accatattct cgaacctctt tttgttccat
    23761 ttgttctaaa tactcgacca catgcatcaa gccaacttgt tgtccggctt ctttttgaac
    23821 taaaacttct tggattccct ttaataccgc cccttttaaa aggtgattgg tacctaacgg
    23881 acttaactca tcaatcatat cctgggctac ttgtttcgca tctactcggg ataaacaaac
    23941 aattggatct aatacgccat aattacgggg atttgtagca tctaacgtga cataactcat
    24001 tttttgcaaa tgttccacaa acaaagggta atgtttctga taatacgggt cttgaatgag
    24061 tttcgtaaac catttgcgct tttctccttt gggatccaca aataaacttt gtacattcat
    24121 aaacgaacta tacaaaaaga gcaatccaac taaaaaggtc ttccctttac cggtttttcc
    24181 agttactgct acatgaggcg catcaaacaa cgcacctttg attccttccg caatagctag
    24241 aagattcaaa aagacaaaac gatttgacgc ataaattaat tcttctaatg gtgttttttc
    24301 tcctcgagaa ccaccatccc ctaatgtatc ttgccgacca atataccagc cggattccat
    24361 acctaattct gatactgctg aaaacaacca ttcgccaatt gcttctacag ttgttcggtg
    24421 cttccaatgc aactgtcgac ccaatggttg ccctaataaa catttataga acaattctcg
    24481 ttgatcattt tttgcacggt ggatatggat agtcgtatga agctgttgaa taatcgttgc
    24541 actattttga cgaacttctt caaccgtttg accgtataca ataaaacatg cgatccaatc
    24601 gacgacaggt tggccattat ctactcgttg ctttaaatgt tttactaatc cccgaacagt
    24661 taataagcgt tcgctaggtt cttcatcgtt ttgaaccaat tccttatgtt ctgttttgaa
    24721 ccgttccgat agccaattta gttttaaacc aatacctccc tgtcctttca agcgtggata
    24781 gtgaattttc attcgtaatt ctgctgggaa agaaagatcc tgtgcaaatt gaaatagatc
    24841 acagtttctc atattttcag gtgtttctcc aataggtaac aacgctaaat aactagtccc
    24901 caactcatct tctaattgaa gaaggccttt atgttccttc ggcatacgta catacgttcc
    24961 ttgattgtct ttcagtactt cccaattatt ttgttcttct actttatgag ccattcctcg
    25021 aatatattgc agacgattga gatacaacag ctcttttttt actaagggac gaatagaaaa
    25081 gctataaagt aattgtgtca cttcttttac ataaggatca ctttcagaaa acaaagtttc
    25141 taaatcatct acttctaatc caaataagcg tgctaaactt cctgctacat ctaaccagat
    25201 tagatttagt acatcataaa aactctccac ggttgctttt tctttgactt tgattcctaa
    25261 aataaaggaa ggacgataga cttgaccttc tcgttccaac aatgtcactg tttcttttgc
    25321 ataatacgca ccaattgctt gactatcggg agaaaatccc tttcctaatg tgtcaaattt
    25381 cgtccgtaaa ttcattgttc gtggatacat caccaactcc acatcttcat aatctgctaa
    25441 aagtaacagt aaacgagcca tcatttcttt tatttcctgt gtcttttttg gtgtgttttt
    25501 tgaaatggtt cgtggaaaaa catgatagta ggcccagata caatcctcgc catttaacaa
    25561 acaatttttc tttaatgctt tcactggttc ttttacaagt accaatagat ctcacctccc
    25621 aattaaaaaa aggaacaacc agttgattgt ccctttttct aatcctctaa ataaacatac
    25681 ttattcgcaa aaagtaattc ttccaatgca ttatttgata ccttgtccca gttataatac
    25741 acacaatcac cattcatcat acgcactaca taacaaaacc aatcccataa ataaaaatgc
    25801 actttttttc catcaggttt tagttttgcc agtgctaaca cacataaata gggtagccct
    25861 ataaaaaaaa caacccctgc tggtggaaaa aaagaaataa cccaaccaat aaatgaacgt
    25921 aaaaataaat attcaaagat tacgaccaat aaaaaagttg ctgcattgac taatgcaact
    25981 ggaaatggaa gacgccaatt gcttgtaatc tgttgtacgc gataaggctc cgcataaaca
    26041 atcgaataat cgtaaggttc ccaatccact tcactatcct cctaatccat aaataatttt
    26101 tcaatgattt taccaaacgc atcaaataca cgcgttggat catttaagaa gaaatagact
    26161 gcggcacctg ctgcgataga tattaatgca tttcctactt ttgatttcat aaaatttcgc
    26221 aatcccagat aaacaaccac cattattaaa aaatagccac cttgcgttaa tataaaatct
    26281 acaattccct tcagtgtcat ttccgttcgt ctcctttcct atccaaccaa ttatgtgtga
    26341 gtttttcaac ataatactga tttgtttttt tcgtaataac aagcgtcatt tgttcttttt
    26401 gtaaaagtga cgtatcacca tctcgaaaga cgactgttgc ttttacaata aaagtttttt
    26461 tcgtttcttt ataacgcaat actttcatct ctacttgatc aactttagct gtctgattga
    26521 gtccttcagg ttctttcatc atgtatgaca tatcttttgc attgtttttc gcatacttat
    26581 ctaagaaatc ttccacaaat ccttccacat ctttcgcttc cgacgcatca accgtttcgt
    26641 ataatgtgtc atcataagta atcattcgtt gtttttctgt attctttggt agtgccgtaa
    26701 aataaggata agcaataata cgacagccct tgtttgaaat agaaaaggga agattgagta
    26761 cttgtgtgat tgtcacactt gtttctttct tattctctgg atccatcgag cgaatatcat
    26821 aagttacttt ataactagct gttggaatgg tttcaatttc ttttaatcca ataaacgaag
    26881 cagcagttaa ttttcgaata cctgaaacac ttcctggttc ttgaataaag ttttgtgcat
    26941 aaaactcttt ttttaatatt tcttgacgtt gttgttcttg ttcatgatta tcgaatgtat
    27001 tcatatacaa agcaacaaaa cgattcataa aggattcaat ttcaggtgtg tacgttaatt
    27061 ctgactgact ttgtacttct ttcttttgta attgttggat ttcatctttc aaaaacgaca
    27121 cttgaacctt cgttttcatg gattgaaaaa atgccaagcc ccctgaaaaa caaagaaata
    27181 aaaccaaaca ccaaatccat ttcactgctt tttttgaatg gattttcctt tgattcggta
    27241 ctttctctgt ataaactttt tctttccgcc gtagttgtcg ttttttcata agctactcct
    27301 tctagtatgt ttttactagt acgtacgatt gacaacgata agcttgtttt tctgaaaaat
    27361 agtccattca attaccctca ttttctattc cttttctctt tcttttatat ccaacaaatc
    27421 cagcaatact tgtcaacact cctcctagaa cagaaagtac aacatgtatt tcttcaccag
    27481 tctttggtaa gacttttgtt tttccatctg ctgttttgac ttcaatattg ccacttggaa
    27541 gtttctttac aggaatcgac aaatccttta ccaaattccc ttcactatct tggattaatg
    27601 gaattccatc ttttacacca acaattgttt gtccaccttc tgcttttagt ggagttgttg
    27661 gtgttggaat atgggtcaat tcctctacat tactagtgac aatcggtaca ctcattcctt
    27721 gagaattatt cgtcgtaatt tcaccgtttg gggttaccga tactgtgact tcttttgctg
    27781 gaacaatagg tttattttct tttggaattg cttctgattt ttcagcaggt tgtttcggtt
    27841 cttcttttgg ttcttcactg gtaaacgaca tcgttggctg ttccgtactt gacgaatcgg
    27901 tattcgtttc agaagaactg gttgttcctg attgttctgg agttgtgact atacttgaat
    27961 ccgttgttgt tgaactgtgc acaccttctt cttcaattgg atcagtcggc aaagttgatt
    28021 caggaatctc tggttctggt gccactggct ctttttcagg tttctctggc tgcaaatgtt
    28081 caggttctgc tggtttttcc gttgttcctc catttagctc tgtctcaaca tctgataatc
    28141 cttcccctgg caatgtttct acaatttctg acttaggtgt ttctacagtt tcatctgcat
    28201 aaaccaatga actccctaat tgaaacgaaa ataatgaaat tcctagtgtt gttacgactg
    28261 ctaatgttgt ttttttcatg attattcctc caatttttta tgcaattcgt gttttgttca
    28321 tagacgccgc actaatatta tctgaacgat aaaagaactc ccgtccgttt tcttctaacc
    28381 caacatatgg attatttttg aataattgaa tcaatagttc actggtgttg tattttggat
    28441 agtttgcaaa aaaataatac tgtgcttgtc gcccatccga aaattcaaca agtaattcat
    28501 aacgataccg cttcaatcct tttcgttgtt ttaaaggatc tctataccaa cttgtccatc
    28561 gttccgttcg tggaccgaga tagacacgat acacatagaa caatgccata ccacatacca
    28621 ccaaaaataa taatcgccac ataaaaattc ccccttttct tctacctttc ttgaatcacc
    28681 gaacattcat accctcggta gagatccgaa aaatccatca ttaatttttc gtataaacgt
    28741 tgttgcaaat agcgattgtt tcgaatacat aattcttcat agccatcaat cacttgatct
    28801 tctttctcat agcatcttgt agctctatga cgctcagctt gcatgacata accaaactct
    28861 gcaccatatc gttctaccgt taataactgt tgactacgca ataatgcaat ataacaatca
    28921 caaaaatgtt cagccgacat ttgatttttc tcacgtttgt gaaataggta ttctttagaa
    28981 agcacattat tcgttaaaca atctgataaa tagcgataat ctggcggtcg gttattttca
    29041 tctgtgaatt tttcccataa taaactgatt tttattcgtt cagcggctag atagtcttgt
    29101 tcgcgtggat aaaattcatc aatcgtacaa agctttttta tcacccgaat aaacaaatcc
    29161 atataatgcg aaacgaactc ttccttttca attggctctc ctaaaaattc cagtgacata
    29221 ccatcttctc cttccttaaa aaataaaaaa gcagaaaccg atttggcttc tacttttctt
    29281 tccttttata gctctttttt acggaagaat ccgcaaatga cattttttca aaaaattaat
    29341 cggcacaagt tcacgaaaac ttggagcaat tttagataga atcacttccc catcttgtaa
    29401 aatacaatca taaaaatcat cagttacttg atacttacgc ttcttacaat aattgataaa
    29461 atagtagcga tcattttcta tttccattcg ttgttctact aagcgcatac gaacatcatc
    29521 atagaaataa gccacaaatc gcccttcaag ttcacgtttt tcaatgggat actcttttgg
    29581 taacaatcta ttttttcgtc gtttatacat tgctggatac cttagaagaa gttcatccaa
    29641 acgtctgtca gtatatggca actgacgaat caatgattcc aatcgttctt tatacagata
    29701 gaataattgg cgcaagcaat agtgtactgc ttctggattt tctaatactg ttggatcacg
    29761 cgaccaatac gcaccaacat aaatcataat ttttttcatt tagacaatca caccagcaat
    29821 actaatcacc gatccacaat aggtagaaca agtggcatag cggccgattt cctctaattc
    29881 cagctcaatc tcttctaaag aaagatacgt ctgttcttct gttggctggc gattcatttc
    29941 ccaagtaaca gttgccaacg agatttcttc cacaagaggt ttttcgttgc taagtgctgc
    30001 cacacagttt tcgtatatct tcattttatc gcagcctttt tttaccgcta aaatcgcagg
    30061 taccggcaat gaaaatcgat aaaagcgaat gaattttagc gttccttttc ttcggctaaa
    30121 ttccaactct tcgagttctt gttcgtaccc cttacgaact tctaataagc gtgcaatttc
    30181 ttgccagttt tctttttcct gataacgtcc agcctttacc gcttgttcaa tataaggaac
    30241 tcgaaaacga aaataagaca aatcaaccat cttttcattt aatgcttcaa ttgcagcctt
    30301 taaatccaaa agacgcagcc attctgttgt cgtatatgca ttcatgcaat tttccctcct
    30361 gacgttccca tccattctag atagctttgt tgctcgtcat ttagttcagc attctcaatt
    30421 ccttcttgta caaagtctct tcctaaaata cgatctgctt cttgtaataa ctttaatgtc
    30481 ggagctactt gtttcaaaaa ccagcgttct gaacgatcta atgagaattt ttctggcttt
    30541 acggtaaaat ccaattcttc catcgaacga aacaattttg cccatcgttc atcaacaatt
    30601 cgtgcatcac tttccagtcg atcccaaaca ataagttttg cattaattaa accaacgcca
    30661 atctctccta aacgtttagg ggaatgatcc aaaataaacg cttctactgc ttgtgttgct
    30721 ttttcattag ctaggcgtaa ctcatatcga ttataaatgc catatttcgt taatgcttct
    30781 atttctgaaa ttttctcacg attcgcaatt tcaaaacgtt tttcgtagaa attgaaaaac
    30841 aatggactct ttcgcgatcc aaaatacaag gataaccctt cgcttcggaa ataaccatca
    30901 tctaaaaaac aaccgccaat attctgaaat ttcttaaagt ccatttctaa taaacctgct
    30961 ttttgttttt ctagtaattc aaagagatca aaatgttctt ctgtttccaa tccccaaagt
    31021 tcatctaacg caatatcgaa tcgactacat tgtactcgtt tataaccagc ttccaacttt
    31081 ccttgatttt tccaaatgga atatagccat gttcgccaat ctgttcgttg ttcacgcatg
    31141 acttcctcat attctcggca accttgaccc gaaaactgta caagtacatt ttgtgttaca
    31201 gtttgttttg aatgccagaa tacccagata tttccacgaa cagaaagaca atcaaaaaga
    31261 tacaagcccg tcggacgtgt ttcaaattct gttagatcca ttcgtaacac acgttgaatc
    31321 actattttgt agtctgtccc aaaaaaggag agacgcaaat agtcgatcat tgctcgaaga
    31381 tctattccgc ttttcatata caatcgaaag cgttccttga ttcgtttttc gaaatcctct
    31441 gggattttct tttttccttg ttccatcttc tttatataat ctaccgagca tcccaataac
    31501 tgtgcaaaac gtttttgaga gagtaatttt ttccctccac ctgaagcgtg aatagcactc
    31561 aatttttttc gataatttcg caattcaata ggtgtcatat tctacctcct ttataaaata
    31621 acaaatttcg attttcttaa tctgctagaa atcccgatat aacaggtggt accccctaat
    31681 ttttctaaaa aggagtactc cttctaacct tagctaaagc cttgatataa ctgatttaaa
    31741 tacacttttt tatttgtgtt atgttatttc tgccccccat attatatatc ggggggcttt
    31801 acacaaagag gccgtgcgca agcgcacgac ctctttgtgg gcacacatgc caagcatgtg
    31861 ctgccacgac tggtctcggt ggcttcgccg acacaagccg cggcagcaca tactcggaca
    31921 atgtgttctc ccatagtccc tatgaaagcc accccgatat ctaaaccaat gaatcttctt
    31981 tttcttgtaa ttctttatac tgttcaggag caaaacactg taaaatcgct aaacgtaact
    32041 gtatgttttt ctctttgcat aaccgatata cctgacgaaa acgcaatata tcctcttttg
    32101 tcaaaacaat ctgatttctc gtaacaaaca atgtctcgcg ctcaattcgt cggaaaacat
    32161 aagaaccttt tgtgtagtat tctactttgt taatccactc gtataatctt cgaaccgcaa
    32221 aatcaggtga aatttcaccc gcagctgaag aaacacgata caaaacttca cccatacttc
    32281 accacttcct tctaaattat aaaagtgtat aactttcctc atctacccca tttgttttcg
    32341 tatttccttt taacgattgc gcttgacgat caatttgttc ttcgtaacta gtaaacgcct
    32401 cttctttcag tacagaaaaa tcttgcacaa tcattgtgtc aaatttttta aattcctcaa
    32461 taaagtgata gtctttgtaa acaaatggcg caaagaattc tacaggcaca tcaaaaccag
    32521 gaaaagcata cccacgacca ggttgatctt tgttataaaa cggcttattc ttaaaatcct
    32581 ctccaaaagt cattaaatag ccaatgggag ataaacggcc aacagaaata cggaacgtta
    32641 aactatctcg aacaccactt ggaaaatatt ccgcatctgg tcgctgcatc gctaaaataa
    32701 catacatgcc tgcttgacga ccttttaata ctaatggctg aattaattta tcaactttgt
    32761 ttctcgtttc catatccaac gaaccataga aagctgccca ttcatcaaaa aagataaaat
    32821 gcggcggtat cccataataa gcataatttt tccctggttc atacttaggc tctttcatcg
    32881 ttttcatata ggtatatcgc tgattcattt cttgtaacgc atttcgtaag caaataatca
    32941 tttcttcatt cgttttgtaa aacacatgtc ctttgaatac atcaatatca cctaatgccg
    33001 ctaaatctgc cgctttccca tcgcaaatat ctactgtccc tacatctaaa caagctttga
    33061 tcaatgaata caataaatag gtttttccgc caccaatcgc accagaaatt aatagatttg
    33121 gcgattgatc atatacccaa tccaatgttt tcattaaatg aatacgtcca ttttctgcta
    33181 tacattgatc aataggaatc cgaccagaat cggaaagaaa tacataattg attcgggctt
    33241 tttctcgttc ttcacgaatt aaatcacgca aaaatacttc ggataataag ctacccattt
    33301 ttaaaaatcg ttcgtggaaa cggccaccat ctaaaggaaa agaaaccact aattcttgtt
    33361 tcttttgata aaaataaact tttggtaatc tcattttttc cttcgtttgt ttctcaccaa
    33421 cttgcttctt tgtataaaca aaccgattgt caattaacat tcgagcaagt aactgacgcc
    33481 aataaacaga agcaaaatat ccttccttta gtcgcgacca acgcatcaaa cctaaaatta
    33541 atccactaac cagcaacaaa tacatcacaa tactgattcc aggaataaga tacatttgcc
    33601 aattcgaata cgtatttaaa cgaggataaa atactttcca actaaaccaa cttaatagaa
    33661 tcaaaaatgg taacagaaag aaaccataat aacaaaacca taaataacga ttgaaataac
    33721 gaattcgtcg accacgatac ttaaaaaaat gaggttccaa ttgtcacatt cctcctttct
    33781 gtttttactt ctcctgtgct aaaaattttt gacaaaaagc aactaatact ggaatatcac
    33841 atttaataaa aaaccataat cgaaaaagat ttaatcgatt tttatatggg tgtgcacaca
    33901 tattacatat tcgtactaca cgaatccaaa ccacttcctg atggtcttgt ttccactgat
    33961 acggtaatga aaaacgaaca agatcacgta aaaacataca ctgcccaata accgcacaaa
    34021 tatactcatt tgtttgtata aaaatcgttg gattgtctcc aaaatactca atcaattgtt
    34081 ggatattttg acaacaaacc ataatctctc gaagtgtttg attggttttg tccagattca
    34141 aaatctcccc ttccttctac cttaaataga aagagtagga agacttcctc ctactcttta
    34201 aaaaacttat tttttctctt tttccttaga atcagcactt ggttgttttt gatctaaacc
    34261 tttttcttgt gattgtttcg gattagaatt atttgttgga gcttctactt tctctaatcc
    34321 tttcgcataa atcactaaat ctgaatattc acgattgcca tcttgtgctg gaatatatac
    34381 cacatgatat tctggatcaa ttaatttcac aggatcacgt cgtttaaaac cagtcaaatc
    34441 tacatctgct ggaaatttaa ctcgtcgtgc aatctttggt tcatctggat ttccaaaaac
    34501 taattgtgct cgctgtgatc gaatttctcc cgtataatcg cctgtttcat tatcaaatac
    34561 acgatcggac tcttctaatg cgccaaagcg tacggctcct atacaagttt catctaatgc
    34621 ttctgtaata tcataattat ttggaaattt taatcccatc ttacaaaacc tcctcaattt
    34681 ttacaccagt taaaaagaca ccgacatctt gatagccagt tgttcgacgc tctgtttgtc
    34741 ccatatcatt tgtttcacga ctattcactc tttctttacg aaaaacaaaa taagcttctt
    34801 caaatgaaat acgtacctct ttttctggta atcgtaatct tttatcctct tcagctgtga
    34861 gataaacaaa aaaagcaggc tgttcttctc gcgaacattc tgcttgtaat tgaacatatc
    34921 gcccagacac accattaatt tcctttggtg gattaggttc ccgtgaaccc aataaaaata
    34981 cctgacctaa aaactctttc ttcatacgca atgctttttc tgccatatca tttttcctcc
    35041 tcttaaaatt cacaatcaat tctaaacgac tctatattac acaatggaaa gtaatgttta
    35101 acaacaaatg gctcatcact aggagacata tctactatta acatcccgtc tcgttcatgc
    35161 acagaaatat cagtacactc ctcatacgta cattcttttc cactcaaaaa acaaatattt
    35221 atatttttct tttccatatt cttttttcct ccattatttt cttttctgta aaaaccaagt
    35281 aataattcct tttccatctt ccaacacaaa ctgatgatat atttccctag caagaatcca
    35341 cacacatgct aaaataaaga aaataataat actactaaag atgcactcat ccttgctgtc
    35401 aaacggaaaa agatatatca taattgcctc ccccataatt aagaaaccaa ttgatacaaa
    35461 caaaaactct accatcatac aaaagaatat ttcttccaac tcttttacat cctttctaac
    35521 tttttataaa aaaggagatt atttttataa acacttttat tcaacaacta ttttcaaatg
    35581 aattacacat tatcatctca agtatagttt caatcgtcac tattgtttgg tccatttcta
    35641 gcttttattt aaaacatcgc tatacaagaa aaaagaaaag cactgaaacc ctaacaaaag
    35701 aaatgtttat tccatttaaa tcttcaattg aaaattatct atttcaaaaa attacaagaa
    35761 aaaatattct agaacgaaaa caaacaattc aacatctaat ccaaacagtt gaaaataaaa
    35821 atatagactt ttatttgcct tatgaactta tttattttgc caaagaaata caaaaaataa
    35881 ttaacacaaa taaacattcg aaaaaaatcc tcttcaaagt acaaagaatg tattttgaat
    35941 tctcatactg ctacctagca gaactaaata aagtaagaaa gcaattagga gtagctaaac
    36001 gtgatttctt ctatagaaaa aaagaaactg tattatcaca taattatatt ctatctcttt
    36061 tggatcctta aattcttatt tattacactc agtatttctt ggtgtatcct catgatcatt
    36121 tactactcaa cacaataatt tatttgcctt tgataaaaaa ttctttctaa aaactaaaca
    36181 agcggctagt tgcctaaccg cttgttcgtc ctctttagtc tctgacttta tacaaatcat
    36241 aaccaatcaa tcctgccgca aggacaaaga tcataccaat catgatccat aatacattgt
    36301 cttcagagcc tgtttttggc aacataggta ccattgcagc ttgtttcact tcttttacag
    36361 gttgtggttc gtttggtttt actggttgcg gtggttttgg tgcctccaca aattgaaccg
    36421 tttgatcttt gtttgttaga tcgacgttat gttttgcgac ttcttcaaac gtttcttttt
    36481 catcttctgt tttggcttgg agccattccg caaaactcaa tccttttcct gcaaggttgc
    36541 gaccgtcaac gatgaattca aacaagaatt ccatcatttc tttatcagcc ataaagactt
    36601 tttccgcatg tgttccttca aatggggtgc cgtctggtgt taagtagcct tgaacaatca
    36661 gtttgtaaat ttgaccgatt gctaacccat taacttgaac ccattcgaca accttttgat
    36721 ttggtaaagc taagatctct tttccaccat ttgcattggt agcttttgtc gtaatggttg
    36781 gactgacgat tttgatggtt tggtctttgt cggtaatatc tgcgtgagaa gccaataata
    36841 cgccttctct aaacagatct tcaaaaacta cgacttcttt gcttcgcaac gcatgtgcgt
    36901 ctaggataaa gtctagattg actgtaccat cagttgtttc tgccacaaac gttaaggtac
    36961 ttgagacttc ttttccatct actagcactg gtttttccgt tactttgtcc attagaacac
    37021 cgttgactgt gtatgttttt ccaacgatca aatcgtggta tttcacagcg tctgtaatgg
    37081 tgactttcgt taatggatta acctctttca agccagtggg tttatgtgta gccgttgtac
    37141 gtacacttgg gttcgttact cgtaccgttt gacctttgtc gttgatgtct ttatggtttg
    37201 ctagttcttc gtcatttcta aaaagacttt caaacacgac catttcacga ccatttaaac
    37261 ctgtcaaatc aaaggtaaat ggtacgatca ccgtttcatt ttctgctttg gcaataaagc
    37321 caacagcact ttctaccggt tgaccatgaa caagtaaggc ttcaccagtt gctttatcca
    37381 ttaagattcc tttgaccaag taagcttttc ctggaaggat ctcattaatt tcaacggtat
    37441 catttaaggt gaacagcccc gttgggtctg cgatcgtcaa cccgtcttca aatacagctg
    37501 tggtatgaac cgttggattg tttacccgaa cactttgatt gtcatcattg atatccacat
    37561 gtaccgcaac ttcaatgcca tcacggaaaa gtttttcaaa gacaacgatt gtttgacctt
    37621 tcagaacact agcattaacc acaaattcaa gatcttttgt gccattggct ttgtctgcaa
    37681 taaaggtcaa gatacttgtg atttctttgc cttcttgtaa aattggcgca ttcgttgcct
    37741 tgttcatcaa tacacctgaa atagtgtatt ctttgccgac aaacaggttg gtataagaaa
    37801 cactatcttt aatgacgata gtttctaatg gatcaaccac tttttcacca tcggctttat
    37861 ttgacgcttg tgtttctaat tttggtggaa tcaaatagtt acgtacagaa accaataacg
    37921 tttccccatc atctggcgca ttttgtctga atgtaatagt gaatgcttgt ttgcctgtat
    37981 ttggttggaa actttctaat ggtacatttt cggtaaagta atagttattg gcaaccagac
    38041 cttttactaa aatctcacca ttttgatcgg tggtatattt accaattgct tgaccttttc
    38101 cgttatatag cgtaaacgtc acaccggcaa gtggtgtttt cttcttgttc cattcttcat
    38161 acttgaacaa ttttacactt ttccgtgctt gacggttcgc aaaatggatc actttgatcg
    38221 gttctgtatc attggtaatc gagaacacta atgggttttg attcaaaacg taatttacgg
    38281 gtgctttgat ttcttccaaa cgatagcgct cttttccata agtaagtgta ccgttagtta
    38341 ggaaaaagcc tttttcattg gttttaaaga tatccgttgt ttctgtatca tttggaatct
    38401 ccattgtgac ataacgattg gctaaagtat caaaaatctt gaattctgta ttggcaagtg
    38461 gaatcacttt tcctgtttct tgatccactt tcaccacttt aagacgttgt tcaattactt
    38521 tatcttctaa aacataatgg aaagtttgcc cattttcaga aattgtgact tcaagtggtt
    38581 caaatagttt atacccttct tttcctttgg tttcagaaag aatgtaggta tcaaagacaa
    38641 cagtattttt accaaaagta gcgtaaccat cattgtctgt cacaacggtt ttcaccactt
    38701 tgccagttgt ttttgacgtc aaagagattt caatgccagc taattttggt ttgttatctg
    38761 gattggttga atcgactaaa tctttattag caattttcac taaatcaatg gttcctttga
    38821 tcacttgatc ttttgcggtc gcattgacag tttttcctgg ttcaacaaca atttcaattg
    38881 tttcatcaga aagtgcatag cctttcggcg ctttgatttc ttttaaaaca tatttgccag
    38941 gatcaagatc tttaatttct gcctgtactt tgccgttcat atttttcaag gtaatttctt
    39001 tgatcttttt cccatctgct tgatacaagc catacaccgc attttctaaa gaagctgcgc
    39061 cttgtgcttt cacaccggtt tctgcatctt ctttaaagac acgaatgttt ccctgtttca
    39121 aaatagtcaa attgacaaca gagtcaccag gttcaaaagg attcaatata ccaattgttt
    39181 gggcacctgg acgacgatac gctaatggcg tacccaatac ttcaatctga ttgaaatgaa
    39241 tttttgcctt ttctggtgct tcttttgtga ctgtgatcca tgcttcattc ccatgaatag
    39301 atacattgat cccttttggc ttttgaatgc cttttacacg ttgtagaaag tcattgccac
    39361 tttttacttt taacgtttcg ccaattttta tcgtatgtgt ttgattatga aaagtagctc
    39421 ttacatcaaa attattaagc agacgttgaa tttctgcttt tcgacgatca taatcaacac
    39481 ggatattttt gattgggatt tttgtgccca aagcttccca aatcataaat tgtgccgcaa
    39541 tgtattgttc atcggatttg tctttatttg tgacatatcc taaataagag atcaagcttg
    39601 tttttttccg tacatttgaa tttttcaaat agttttccaa agcagtagct gtataaactt
    39661 gaccttctgc ggctgagaca ccaggctcaa tacaaaacgc cacttgtcca ttaatctcaa
    39721 aacgaaccat ataatttaaa gtgaagtttg gattgttcgg actagaagta acaccaatat
    39781 tttcgcccca atcaattttc cgaccaccta aaacagctgt attggttgca cggcgaacac
    39841 gaaccgaacg attggttggt actgttacta acagtgtagg tgtacctgtt tttggaatgt
    39901 cctgctcttt tttcttttga tcttcaatac tagccgtaga ttctttgttt agtttcgttg
    39961 gttgattttg actagctgcg gttgaagaga ttgttttttc aacgttttcc tcattttttc
    40021 cttctttgtc agcagtagcc gctggttgtt ccactgtttc ttctattcgt tgcttttctt
    40081 ccgcttgttt ctcagaagac tctggcttca cagaaggcgt tgcttcggca gctttttctc
    40141 cttctggagt ggacgtttct gaagcagggg ttgtaattga ggcagtttct tcaactggtt
    40201 gtgaagtaac aggactattc cctgggtgtt gtgtttcccc aatcgtctga tcgagagtcg
    40261 ttttggaatt aaccgttggc gcttctgtca caggggcctg ttcaacagtg gcttcaccag
    40321 ctgggacccc ggcttctgct ggttggactt ctgtcacttc agaagcgact tgggcaatgg
    40381 cttccaccgt aggttgtgta gccgtcgttt cttctgcatg tgcttgagca gttcccacca
    40441 agaaacagaa ttgagataag gccaccaatg cgagtgcttt caaccaattc attttcgttt
    40501 ttgcatacac aagaattcct ccttttcctg agaattttat ataataaaac aaaacaccgt
    40561 taccaaaagt gataacgacg tgaactgtct cttctatttt tatctggcgc tcttatctga
    40621 acacatttca tcaatgattt taccctccca ctataattac agaacaaaaa aagaacctgc
    40681 ttcgtggaaa tcaactgtcc atttcccaat tggaatacca gctttattgg caacttcacc
    40741 atcaccaaat atcatgccat accaattact accttcatcc ttaatttctt tccaaccata
    40801 tgacatagct tcatctaacg aatcaaaaat catgccactg ttgccaattg ttgcaggatc
    40861 tggcttaaga tcttctggaa tagaaatatc aatcacacca taatgatccg attccccagt
    40921 attattgtca ttactagcta ctggtttggt tgttctactg acttattttg atacaacaag
    40981 tacgcttatt cttcttttac ttctccgttt tcaatttccc attcaaacca ctccgcaaag
    41041 tcttgcttaa aaccatctac tcgataccta ttttgtaaat aggcatcttc tgacgtcgta
    41101 ggatctaaat aggtaggttt attctctgga cgagcataga cacctaccca tgtttcgtaa
    41161 tctccttcaa aataactatc taccaaatag cctttttttc taagaatctc ccgaacttga
    41221 tttttaatta ttgatacttg ttcaaaacca tcactcattc tatcacctca aataactcta
    41281 gattctcata aatattcccg acaatttcaa tcctatcaat gatctctaac aaatcgctac
    41341 ttatgcttcc ccattcgtat aagaatttcc cttccgcaaa ataaattttc cataaacttc
    41401 ttcatgatcg tcccaaccaa tatcactctc gacaaattcc acgttgttct tatcttttag
    41461 ctcagtaaat gccataagtt caacatgttt tccaacctcg aaacaatcac accacccatc
    41521 aaccgttaca cgtgtgcctt caatacctaa gaaactttgt cctcgtgtat taaacactaa
    41581 aactttccgc attttttgct cattaattaa tcacgcgcga aatctaggaa tcacaacttg
    41641 ttcgcttctt ctttataaaa taaaaaaact ccaataatcg gaaattacta tcttttcctc
    41701 attccttttt taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt
    41761 ttgaagaaaa actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc
    41821 ttcagatcct tttactagaa cttgtataca aaaattgttg aatgctgata caatttgcgg
    41881 ttacttgaaa aaacgataga tgtaaaaaga taaatagaat cattttttta ttctatatca
    41941 gtctatgatt cattgcttat caatcaccca acccattcga tgccgttttt ttaagagaaa
    42001 tttaaaaaag accgtacgaa tctcgcattg tccgattact tttctagaaa agatccccaa
    42061 ttctgtataa ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca
    42121 atctacccac tatgaccatt actacgtcca acacaaccga catattaacc acgtaagtat
    42181 tccctcacag atctccgaat ttccatggta tggattccca tcctgacgct gcagaggaca
    42241 acatatttca tgtggctctg tacccttttg atttggaaag actacaaatc aatactcatc
    42301 tataacttat caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca
    42361 tacctagccc attttatcta gctggtattc ataacagtta tttcttgtat tacttcttcc
    42421 gcttgtgttg ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa
    42481 ctttcaacaa acccttt
//
LOCUS       DACEKQ010000011        41193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEKQ010000011 DACEKQ010000000
VERSION     DACEKQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.011"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..423
                     /locus_tag="IW671_000555"
     CDS             139..423
                     /locus_tag="IW671_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0897566.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(704..1123)
                     /locus_tag="IW671_000556"
     CDS             complement(704..1123)
                     /locus_tag="IW671_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0897567.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1125..1709)
                     /locus_tag="IW671_000557"
     CDS             complement(1125..1709)
                     /locus_tag="IW671_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0897568.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1756..3231)
                     /locus_tag="IW671_000558"
     CDS             complement(1756..3231)
                     /locus_tag="IW671_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0897569.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3412..3723
                     /locus_tag="IW671_000559"
     CDS             3412..3723
                     /locus_tag="IW671_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897570.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3838..6174)
                     /locus_tag="IW671_000560"
     CDS             complement(3838..6174)
                     /locus_tag="IW671_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0897571.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6316..6984
                     /locus_tag="IW671_000561"
     CDS             6316..6984
                     /locus_tag="IW671_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897572.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6987..8030
                     /locus_tag="IW671_000562"
     CDS             6987..8030
                     /locus_tag="IW671_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0897573.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8267..9148)
                     /locus_tag="IW671_000563"
     CDS             complement(8267..9148)
                     /locus_tag="IW671_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0897574.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9307..10035
                     /locus_tag="IW671_000564"
     CDS             9307..10035
                     /locus_tag="IW671_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0897575.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10191..10508
                     /locus_tag="IW671_000565"
     CDS             10191..10508
                     /locus_tag="IW671_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0897576.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10501..11109
                     /locus_tag="IW671_000566"
     CDS             10501..11109
                     /locus_tag="IW671_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0897577.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11125..11955
                     /locus_tag="IW671_000567"
     CDS             11125..11955
                     /locus_tag="IW671_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0897578.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12093..12581
                     /locus_tag="IW671_000568"
     CDS             12093..12581
                     /locus_tag="IW671_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0897579.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12632..13504
                     /locus_tag="IW671_000569"
     CDS             12632..13504
                     /locus_tag="IW671_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0897580.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13680..14042
                     /locus_tag="IW671_000570"
     CDS             13680..14042
                     /locus_tag="IW671_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0897581.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14051..14803
                     /locus_tag="IW671_000571"
     CDS             14051..14803
                     /locus_tag="IW671_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897582.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14817..16619
                     /locus_tag="IW671_000572"
     CDS             14817..16619
                     /locus_tag="IW671_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897583.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16896..17936
                     /locus_tag="IW671_000573"
     CDS             16896..17936
                     /locus_tag="IW671_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0897584.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17988..20132)
                     /locus_tag="IW671_000574"
     CDS             complement(17988..20132)
                     /locus_tag="IW671_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0897585.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20489..21862)
                     /locus_tag="IW671_000575"
     CDS             complement(20489..21862)
                     /locus_tag="IW671_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0897586.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IW671_000576"
     CDS             complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IW671_000576"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0897587.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IW671_000577"
     CDS             complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IW671_000577"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0897588.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23310..24323)
                     /locus_tag="IW671_000578"
     CDS             complement(23310..24323)
                     /locus_tag="IW671_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0897589.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24404..25003)
                     /locus_tag="IW671_000579"
     CDS             complement(24404..25003)
                     /locus_tag="IW671_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0897590.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25189..25890
                     /locus_tag="IW671_000580"
     CDS             25189..25890
                     /locus_tag="IW671_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897591.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25900..28611
                     /locus_tag="IW671_000581"
     CDS             25900..28611
                     /locus_tag="IW671_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0897592.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLNKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29062..30690
                     /gene="treC"
                     /locus_tag="IW671_000582"
     CDS             29062..30690
                     /gene="treC"
                     /locus_tag="IW671_000582"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0897593.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30750..31931)
                     /locus_tag="IW671_000583"
     CDS             complement(30750..31931)
                     /locus_tag="IW671_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0897594.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32183..33706
                     /locus_tag="IW671_000584"
     CDS             32183..33706
                     /locus_tag="IW671_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0897595.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33775..34503)
                     /locus_tag="IW671_000585"
     CDS             complement(33775..34503)
                     /locus_tag="IW671_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897596.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKFFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34516..35796)
                     /locus_tag="IW671_000586"
     CDS             complement(34516..35796)
                     /locus_tag="IW671_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0897597.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35937..36539)
                     /locus_tag="IW671_000587"
     CDS             complement(35937..36539)
                     /locus_tag="IW671_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0897598.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36784..36990
                     /locus_tag="IW671_000588"
     CDS             36784..36990
                     /locus_tag="IW671_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897599.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37302..38021
                     /locus_tag="IW671_000589"
     CDS             37302..38021
                     /locus_tag="IW671_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0897600.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38102..38785)
                     /locus_tag="IW671_000590"
     CDS             complement(38102..38785)
                     /locus_tag="IW671_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0897601.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39003..41189
                     /locus_tag="IW671_000591"
     CDS             39003..41189
                     /locus_tag="IW671_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0897602.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13364 a         7307 c         8259 g        12263 t
ORIGIN      
        1 tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg
       61 aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat actagatgta
      121 aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc
      181 gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt
      241 gctggaagag ttaaagtcca agcaactttt gacaatgtgc catatgaagg cagtatcgtg
      301 aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat
      361 attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa
      421 taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca
      481 gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt
      541 tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag
      601 ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt
      661 ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac
      721 catctttata ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa
      781 tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat
      841 ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca
      901 cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac
      961 gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat
     1021 ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg
     1081 tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc accctgccaa
     1141 cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta
     1201 acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc
     1261 acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca
     1321 cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc
     1381 aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatgcttgc cggtacgatg
     1441 atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa
     1501 taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag
     1561 ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt
     1621 ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt
     1681 tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca
     1741 gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc
     1801 acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact
     1861 tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg
     1921 atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa
     1981 agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt
     2041 cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc
     2101 cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg
     2161 attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc
     2221 ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat
     2281 gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt
     2341 agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc
     2401 ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat
     2461 acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc
     2521 tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat
     2581 ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc
     2641 gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt
     2701 atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc
     2761 tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg
     2821 tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg
     2881 tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt
     2941 caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact
     3001 agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat
     3061 agttggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac
     3121 accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag
     3181 tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc
     3241 ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact
     3301 tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat
     3361 gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa
     3421 atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt
     3481 acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat
     3541 gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt
     3601 aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga
     3661 aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta
     3721 taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc
     3781 tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta
     3841 ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc
     3901 gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag
     3961 ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc
     4021 tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc
     4081 gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc
     4141 ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac
     4201 taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt
     4261 cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc
     4321 tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata
     4381 aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc
     4441 attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac
     4501 gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat
     4561 tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt
     4621 caattcatca gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc
     4681 agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat
     4741 atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt
     4801 gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata
     4861 acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg
     4921 tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag
     4981 agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc
     5041 acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc
     5101 gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc
     5161 ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc
     5221 caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt
     5281 cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag
     5341 aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc
     5401 tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt
     5461 tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa
     5521 gatcttacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt
     5581 atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg
     5641 gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc
     5701 attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg
     5761 ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg
     5821 tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc
     5881 tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc
     5941 atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga
     6001 atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg
     6061 acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt
     6121 ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac
     6181 ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt
     6241 atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa
     6301 aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg
     6361 ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag
     6421 gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg
     6481 ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct
     6541 gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga
     6601 gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca
     6661 ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta
     6721 gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa
     6781 tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg
     6841 atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc
     6901 aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga
     6961 ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct
     7021 attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta
     7081 ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat
     7141 acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg
     7201 ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa
     7261 cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt
     7321 ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact
     7381 ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc
     7441 aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta
     7501 tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg
     7561 aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag
     7621 taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg
     7681 ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg
     7741 ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag
     7801 ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag
     7861 atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc
     7921 taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca
     7981 gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat
     8041 aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta
     8101 gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag
     8161 aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc
     8221 gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt
     8281 gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa
     8341 atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata
     8401 gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga
     8461 tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac
     8521 acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct
     8581 ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa
     8641 ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga
     8701 aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga
     8761 cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga
     8821 ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat
     8881 tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat
     8941 tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc
     9001 gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc
     9061 cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa
     9121 gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca ataaagaata
     9181 ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc
     9241 gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt
     9301 tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt
     9361 ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt
     9421 ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct
     9481 gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg
     9541 ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag
     9601 cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt
     9661 gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt
     9721 ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg
     9781 tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg
     9841 gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc
     9901 ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct
     9961 gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt
    10021 atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa
    10081 atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt
    10141 ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc
    10201 agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt
    10261 atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat
    10321 atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg
    10381 aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa
    10441 ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa
    10501 atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt
    10561 tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag
    10621 atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt
    10681 tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg
    10741 gtttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg
    10801 tttatattgg ttattggggg actattggtt gctttactag cgctcatttt tagtatctat
    10861 gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg
    10921 tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga
    10981 agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg
    11041 atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg
    11101 ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt
    11161 ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt
    11221 ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag
    11281 gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg
    11341 gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat
    11401 ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac
    11461 acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc
    11521 gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag
    11581 acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat
    11641 gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa
    11701 aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac
    11761 ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt
    11821 gaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag
    11881 aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac
    11941 aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct
    12001 tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa
    12061 atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa
    12121 agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct
    12181 atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat
    12241 tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt
    12301 attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat
    12361 ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct
    12421 tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac
    12481 cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag
    12541 aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt
    12601 tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa
    12661 tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta
    12721 tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat
    12781 cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt
    12841 gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt
    12901 ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt
    12961 caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga
    13021 tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga
    13081 gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt
    13141 gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg
    13201 tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac
    13261 agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca
    13321 aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg
    13381 acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc
    13441 aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa
    13501 gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc
    13561 tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa
    13621 ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag
    13681 tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa
    13741 tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta
    13801 caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc
    13861 ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac
    13921 cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt
    13981 atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt
    14041 agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa
    14101 caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt
    14161 tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac
    14221 attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa
    14281 aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa
    14341 tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt
    14401 cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca
    14461 tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc
    14521 acgtgcattg atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga
    14581 ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt
    14641 atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt
    14701 catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt
    14761 ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc
    14821 tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct
    14881 caggattagt tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag
    14941 cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg
    15001 ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga
    15061 tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac
    15121 agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg
    15181 tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata
    15241 tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg
    15301 ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag
    15361 cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc
    15421 ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg
    15481 ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt
    15541 tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt
    15601 cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata
    15661 cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag
    15721 gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag
    15781 tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt
    15841 ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg
    15901 cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag
    15961 aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg
    16021 aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac
    16081 tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga
    16141 aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag
    16201 agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg
    16261 aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt
    16321 tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag
    16381 gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag
    16441 caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca
    16501 tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca
    16561 tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa
    16621 aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc
    16681 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga
    16741 ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa
    16801 attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg tatgctggaa
    16861 tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg
    16921 gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg
    16981 atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga
    17041 tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa
    17101 tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg
    17161 aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag
    17221 ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc
    17281 agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata
    17341 cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag
    17401 tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg
    17461 acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc
    17521 ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat
    17581 taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc
    17641 catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg
    17701 aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt
    17761 ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat
    17821 ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg
    17881 aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata
    17941 aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa
    18001 ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca
    18061 gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa
    18121 tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa
    18181 aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc
    18241 ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt
    18301 tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg
    18361 cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc
    18421 gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg
    18481 gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg
    18541 tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat
    18601 gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat
    18661 ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg
    18721 atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg
    18781 aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa
    18841 gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata
    18901 gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata
    18961 tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt
    19021 tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg
    19081 atataatgac cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta
    19141 tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg
    19201 attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca
    19261 acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga
    19321 cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct
    19381 ctctgagtat ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg
    19441 ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta
    19501 cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga
    19561 tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa
    19621 ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca
    19681 gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc
    19741 gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa
    19801 tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa
    19861 tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg
    19921 gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt
    19981 tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga
    20041 ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca
    20101 gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga
    20161 ttttctgtct tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag
    20221 ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac
    20281 gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg
    20341 ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt
    20401 atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa
    20461 catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc
    20521 cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc
    20581 agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga
    20641 tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag
    20701 caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg
    20761 caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc
    20821 gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa
    20881 cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt
    20941 ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc
    21001 cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg
    21061 caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa
    21121 atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa
    21181 agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa
    21241 tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc agattcggtt
    21301 caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag
    21361 cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa
    21421 tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac
    21481 cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca
    21541 aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac
    21601 gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat
    21661 cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta
    21721 ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat
    21781 tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt
    21841 caggattcat tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta
    21901 aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc
    21961 ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat
    22021 ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc
    22081 taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat
    22141 tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg
    22201 ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt
    22261 tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg
    22321 gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag
    22381 gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa
    22441 tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg
    22501 ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat
    22561 acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat
    22621 tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag
    22681 ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc
    22741 ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat
    22801 aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt
    22861 atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc
    22921 ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt
    22981 cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt
    23041 ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg
    23101 aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt
    23161 agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta
    23221 tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag
    23281 ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt
    23341 tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct
    23401 aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt
    23461 ttcggcttga tatactcgca catatctata ttatcatagc caataatgct gtagtcttca
    23521 gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt
    23581 aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct
    23641 tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca
    23701 agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag
    23761 acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg
    23821 cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca
    23881 atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa
    23941 gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct
    24001 ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt atacaagata
    24061 tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca
    24121 ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct
    24181 aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt
    24241 ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg
    24301 attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc
    24361 atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga
    24421 tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta
    24481 tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat
    24541 ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac
    24601 cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa
    24661 aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat
    24721 ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag
    24781 tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag
    24841 cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt
    24901 gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag
    24961 tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa
    25021 ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg
    25081 attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat
    25141 tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc
    25201 gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac
    25261 gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca
    25321 ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc
    25381 attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga
    25441 acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa
    25501 aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg
    25561 caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag
    25621 cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa
    25681 ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct
    25741 agcagacaac acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc
    25801 gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat
    25861 ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt
    25921 aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt
    25981 gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa
    26041 acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt
    26101 ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca
    26161 ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat
    26221 aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca
    26281 aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat
    26341 cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct
    26401 ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat
    26461 tattttgtct atttacctga atccgttttt aaaacagata tttattctgt cttatatctt
    26521 gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag
    26581 caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag
    26641 ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa
    26701 gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag
    26761 tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct
    26821 caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg
    26881 gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aaataaacca
    26941 tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct
    27001 gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg
    27061 atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta
    27121 aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta
    27181 gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc
    27241 atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg
    27301 ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc
    27361 gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag
    27421 ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat
    27481 caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc
    27541 ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt
    27601 tctgtttcag ccaaacaatt tggaacgatc attgactatc aagcaatcgt tacattggat
    27661 tcatccacgg aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta
    27721 aatgagatcc tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg
    27781 caaagtctga cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta
    27841 actgatcttg aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa
    27901 gtagcagaac tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa
    27961 aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat
    28021 atgtcccctg aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg
    28081 ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc
    28141 acagacgctg tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca
    28201 atggcgaact tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt
    28261 atcgtcttgt ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca
    28321 ataaaagtcc ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc
    28381 atctttactc ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc
    28441 atcttgcaac aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct
    28501 tatgtcttat cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat
    28561 ttcaaattaa agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg
    28621 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat
    28681 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg
    28741 ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat
    28801 gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa
    28861 gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt
    28921 gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc
    28981 ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg taataaatca
    29041 gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc
    29101 aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa
    29161 ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc
    29221 cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt
    29281 acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg
    29341 ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca
    29401 ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca
    29461 aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta
    29521 tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc
    29581 aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat
    29641 gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat
    29701 acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg
    29761 gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg
    29821 tatacagaac ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc
    29881 gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga attaaagcga
    29941 ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt
    30001 aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa
    30061 caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat
    30121 atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac
    30181 gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg
    30241 tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac
    30301 acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa
    30361 gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa
    30421 ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa
    30481 gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta
    30541 ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc
    30601 aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca
    30661 taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag
    30721 ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa
    30781 gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg
    30841 aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc
    30901 acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc
    30961 tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca
    31021 aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa
    31081 cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa
    31141 atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct
    31201 ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca
    31261 atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac
    31321 tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa
    31381 gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc
    31441 caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat
    31501 tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa
    31561 tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga
    31621 ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact
    31681 gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac
    31741 aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg
    31801 aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct
    31861 aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt
    31921 tcttttttca ctctttttcc tcctttatct ataacttcat tatagacaaa agcgggtata
    31981 aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc
    32041 atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg
    32101 ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa
    32161 gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt
    32221 agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt
    32281 gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga
    32341 aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc
    32401 taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga
    32461 aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc
    32521 gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca
    32581 cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc
    32641 aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga
    32701 agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta
    32761 tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa
    32821 ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga
    32881 agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa
    32941 ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc
    33001 aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa
    33061 tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga
    33121 gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg
    33181 ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca
    33241 aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa
    33301 attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac
    33361 agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac
    33421 aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa
    33481 ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat
    33541 tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg
    33601 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
    33661 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
    33721 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
    33781 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
    33841 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
    33901 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
    33961 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
    34021 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
    34081 tttttggatt tgggacattt gaaaatcttc gaaaaatttg ctcaggatat gctcgacatt
    34141 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
    34201 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
    34261 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
    34321 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
    34381 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
    34441 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
    34501 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
    34561 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
    34621 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
    34681 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
    34741 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
    34801 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
    34861 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
    34921 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
    34981 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
    35041 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
    35101 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
    35161 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
    35221 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
    35281 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
    35341 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
    35401 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
    35461 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
    35521 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
    35581 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
    35641 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
    35701 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
    35761 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
    35821 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
    35881 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
    35941 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
    36001 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
    36061 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
    36121 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
    36181 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
    36241 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
    36301 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
    36361 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
    36421 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
    36481 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
    36541 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
    36601 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
    36661 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
    36721 gctttttcga gtgatttatg ttaaaaaatt actgaccttg taaaaggtga tggagggatt
    36781 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
    36841 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
    36901 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
    36961 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
    37021 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
    37081 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
    37141 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
    37201 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
    37261 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
    37321 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
    37381 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
    37441 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
    37501 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
    37561 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
    37621 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
    37681 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
    37741 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
    37801 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
    37861 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
    37921 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
    37981 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
    38041 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
    38101 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
    38161 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
    38221 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
    38281 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
    38341 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
    38401 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
    38461 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
    38521 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
    38581 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
    38641 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
    38701 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
    38761 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
    38821 cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc
    38881 gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac
    38941 tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc
    39001 agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt
    39061 ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg
    39121 cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa
    39181 acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa
    39241 aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg
    39301 gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg
    39361 aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag
    39421 tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt
    39481 ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag
    39541 gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta
    39601 aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact
    39661 attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg
    39721 attccattga taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg
    39781 aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc
    39841 tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc
    39901 tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga
    39961 caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat
    40021 tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc
    40081 ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga
    40141 tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag
    40201 gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac
    40261 aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat
    40321 cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac
    40381 tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc
    40441 aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc
    40501 aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact
    40561 tttctcctaa atatcaaaat atatccgttg cagattatat tttgtcgata agccccaatt
    40621 taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc
    40681 atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa
    40741 tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa
    40801 gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga
    40861 ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag
    40921 gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg
    40981 gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca gtaacgacga
    41041 tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc
    41101 tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa
    41161 ccgggagtcg tgtgaaaaaa ggaaagtgat aga
//
LOCUS       DACEKQ010000012        40677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEKQ010000012 DACEKQ010000000
VERSION     DACEKQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.012"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..963)
                     /locus_tag="IW671_000592"
     CDS             complement(7..963)
                     /locus_tag="IW671_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0897603.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IW671_000593"
     CDS             1137..1697
                     /locus_tag="IW671_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897604.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IW671_000594"
     CDS             1690..2514
                     /locus_tag="IW671_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0897605.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IW671_000595"
     CDS             2524..3141
                     /locus_tag="IW671_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0897606.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="IW671_000596"
     CDS             3159..3842
                     /locus_tag="IW671_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0897607.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5808
                     /locus_tag="IW671_000597"
     CDS             4525..5808
                     /locus_tag="IW671_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897608.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5824..6711
                     /locus_tag="IW671_000598"
     CDS             5824..6711
                     /locus_tag="IW671_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897609.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6728..7564
                     /locus_tag="IW671_000599"
     CDS             6728..7564
                     /locus_tag="IW671_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897610.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7567..8190
                     /locus_tag="IW671_000600"
     CDS             7567..8190
                     /locus_tag="IW671_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897611.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8187..9479
                     /locus_tag="IW671_000601"
     CDS             8187..9479
                     /locus_tag="IW671_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0897612.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9838..9935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10051..10632
                     /locus_tag="IW671_000602"
     CDS             10051..10632
                     /locus_tag="IW671_000602"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0897613.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10635..11942
                     /locus_tag="IW671_000603"
     CDS             10635..11942
                     /locus_tag="IW671_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0897614.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12358..12846
                     /gene="purE"
                     /locus_tag="IW671_000604"
     CDS             12358..12846
                     /gene="purE"
                     /locus_tag="IW671_000604"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0897615.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12830..13963
                     /gene="purK"
                     /locus_tag="IW671_000605"
     CDS             12830..13963
                     /gene="purK"
                     /locus_tag="IW671_000605"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0897616.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13976..15271
                     /gene="purB"
                     /locus_tag="IW671_000606"
     CDS             13976..15271
                     /gene="purB"
                     /locus_tag="IW671_000606"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0897617.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15451..15828)
                     /locus_tag="IW671_000607"
     CDS             complement(15451..15828)
                     /locus_tag="IW671_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897618.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16084..16812
                     /locus_tag="IW671_000608"
     CDS             16084..16812
                     /locus_tag="IW671_000608"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0897619.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16812..17066
                     /gene="purS"
                     /locus_tag="IW671_000609"
     CDS             16812..17066
                     /gene="purS"
                     /locus_tag="IW671_000609"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0897620.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17068..17739
                     /gene="purQ"
                     /locus_tag="IW671_000610"
     CDS             17068..17739
                     /gene="purQ"
                     /locus_tag="IW671_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0897621.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17740..19962
                     /gene="purL"
                     /locus_tag="IW671_000611"
     CDS             17740..19962
                     /gene="purL"
                     /locus_tag="IW671_000611"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0897622.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19947..21386
                     /locus_tag="IW671_000612"
     CDS             19947..21386
                     /locus_tag="IW671_000612"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0897623.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21418..22461
                     /locus_tag="IW671_000613"
     CDS             21418..22461
                     /locus_tag="IW671_000613"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0897624.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22458..23036
                     /gene="purN"
                     /locus_tag="IW671_000614"
     CDS             22458..23036
                     /gene="purN"
                     /locus_tag="IW671_000614"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0897625.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23033..24568
                     /gene="purH"
                     /locus_tag="IW671_000615"
     CDS             23033..24568
                     /gene="purH"
                     /locus_tag="IW671_000615"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0897626.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24584..25858
                     /gene="purD"
                     /locus_tag="IW671_000616"
     CDS             24584..25858
                     /gene="purD"
                     /locus_tag="IW671_000616"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0897627.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25980..26894
                     /locus_tag="IW671_000617"
     CDS             25980..26894
                     /locus_tag="IW671_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0897628.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27027..28592)
                     /locus_tag="IW671_000618"
     CDS             complement(27027..28592)
                     /locus_tag="IW671_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0897629.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28585..30036)
                     /locus_tag="IW671_000619"
     CDS             complement(28585..30036)
                     /locus_tag="IW671_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0897630.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30050..31066)
                     /locus_tag="IW671_000620"
     CDS             complement(30050..31066)
                     /locus_tag="IW671_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897631.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31286..32374
                     /locus_tag="IW671_000621"
     CDS             31286..32374
                     /locus_tag="IW671_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897632.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32428..33954
                     /locus_tag="IW671_000622"
     CDS             32428..33954
                     /locus_tag="IW671_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897633.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33956..35161
                     /locus_tag="IW671_000623"
     CDS             33956..35161
                     /locus_tag="IW671_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897634.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35418..36395
                     /gene="guaC"
                     /locus_tag="IW671_000624"
     CDS             35418..36395
                     /gene="guaC"
                     /locus_tag="IW671_000624"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0897635.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36659..38083
                     /locus_tag="IW671_000625"
     CDS             36659..38083
                     /locus_tag="IW671_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0897636.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38544..39653
                     /gene="pdhA"
                     /locus_tag="IW671_000626"
     CDS             38544..39653
                     /gene="pdhA"
                     /locus_tag="IW671_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0897637.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39656..40633
                     /locus_tag="IW671_000627"
     CDS             39656..40633
                     /locus_tag="IW671_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0897638.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        12985 a         6937 c         9113 g        11642 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca gcaggaggaa
     5821 ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt cggttacgta
     5881 tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat gatcttgtca
     5941 ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat cggcttggat
     6001 aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt cacttttctc
     6061 tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc ttatctttta
     6121 acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc tagtttgatc
     6181 ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa cgggttagtg
     6241 aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga tcaaaaactg
     6301 gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat gatcatcttt
     6361 gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat cgatggagcg
     6421 aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat cattttgtat
     6481 aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt tatcattacc
     6541 caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa ccaagctttt
     6601 tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt gattatggga
     6661 gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg aatgagagga
     6721 ggaagaaatg aataatacag taacgaaatg gtttcaatac ggttttattt tgctattagg
     6781 tctagtcatg atttatccgg tcttatggat gatcgcaggt tcttttaaaa cggatcaaga
     6841 gattatgagt ggttcgctga atttgattcc agagactttt caatgggaaa attacgcaaa
     6901 aggatgggcg gggtttgctg gaatttcatt ttttactttc tttaggaatt cgtttgtcat
     6961 ctctgcagtt tcgacgatag ggacagttct gagctcgact tgtattgctt acgcattatc
     7021 gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc atgttagtaa cgatgatgat
     7081 tcctgcacaa gtgattttga ttccgcagtt tattatttac aatcggctga atttggttgg
     7141 gacgtatgtg ccgctgattt taccacattt ttttggacaa gcatttttca tttaccagat
     7201 catgcaattc atggtcagca tccccaaaga gttagatgaa tcagcgatta ttgacggatg
     7261 cagcaagtat agtgtcttta ccaaaattat ttttcctttg ctgaaacctt cgatcatcac
     7321 gacgatcatc atccagtttt attggaaatg ggacgacttc atgggaccat tgatttattt
     7381 aaacaagccg agatcttaca cggtatcgat tgccatcaaa ttatttgcag acgctggatc
     7441 tagtacgact tacgcgagta tgtttgcgat gtctacgttg tcgctgctac cggtttttct
     7501 gattttctta ttcttcaata aatatcttgt ccaggggatc agtacaagcg gtctgaaggg
     7561 ctgaatatga aaaagccaat agaagagatg tttctttaca agctcaacta ctatgtctgt
     7621 cggatttttt taaccagtct tatcagtaat gctcttttcg ctttagttct aggatgtttg
     7681 ttcactttag gattagtgct gtctgctcgt gttgtctatc tgatttattt tttgatgatg
     7741 tatccttctt attatctatc tgtccattac ttggcagaaa aaaaggtatg ttggaggaaa
     7801 ggaaaaatca aacggatgtg tttcatcatt acgttgtttc agctgtttgt cattattttt
     7861 gcagttgatt ggtatttatt agtgggaaag attggagctt ggtatctgat tccttttcta
     7921 ttgtgtctgt tgctttttgt atcccatagt ttttttagta cgctaattga tatctgcaaa
     7981 gaagataaag cagatagtct tgtttatttg atcaaaagga atatctggct gttgtcaaaa
     8041 aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg gactgttctg gtgtatacag
     8101 catgattttt tactcactgc attactgtat ccagggctgc catttgtcac agtattttgc
     8161 ttaaaagaaa aggagaatga ctttaaatga agaaaaaata tgtacaagtc ggacttggtg
     8221 ggagagcgcg ttttttttat gaggcaattg cctctacgta tcatcaaact tgtgaactag
     8281 tcggtttttg tgatgtatct caaacaagga tggattatgc aaatcggata cttgtgaaca
     8341 agtatggaca gaaaaaagta aagacatgta tgccatcaca atttgagcaa ctactggatg
     8401 acgtacggcc ggctgaagtc attattacgt cagtagatcg aacgcatcat gattatatca
     8461 ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa acctctgact atcgatgagg
     8521 aaaaagctca agaaataata acggtccaaa aacagacggg aagaagcctg cgagtcactt
     8581 ttaattatcg ttatgcaccg tatcacacga agataaagga actgatagaa agtgatgtga
     8641 ttggtgatgt gttttctatc cacttcgaat ggctgttaaa tacgcaacac ggggcagatt
     8701 atttcagaag atggcatcga aataagcaca atagcggcgg gcttcttgtt cataaatcta
     8761 cccatcattt tgatctagtg aatttttggt tgaacagccg tccccaaaag gtatttgcat
     8821 tcggtggatt aaacttttat gggatgcata atgcggagaa acgcggagta gaaatatttt
     8881 acagtcgatg ctatggaagc gaactagcta aagctgatcc atttgctata gacctgtcag
     8941 ccaatgagga attgcgagaa atgtatttag aagcagaaaa agacagtgga tatattcgtg
     9001 accgcagtgt attttccgat gatatctcta ttgaagatac actaggcgta acggtcaaat
     9061 atgaaactgg tgcgattttg acgtattcgc taaacgcgta tatgccgtgg gaaggattca
     9121 atgtttcttt caatggcagc aaaggacgga tcgaaatgaa agtgatcgaa aagtcttaca
     9181 tcaatgcagg tggagataaa aaggatgaag gagcagccgt tcttcagtcc atccatgtct
     9241 atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca agaagcgggc ggacatggcg
     9301 gaggagatcc gcgtttatta gatgatctat ttggtgaaga aaaagaagaa gatccgtgga
     9361 atcgggcagc aactcacgta gatggtattt tgtcgatttt gacaggaatc gcagcaaatc
     9421 attctattgc ttcaggaaaa gcagtagtga ttgacgagct tgtcagctta aaagaataag
     9481 aatagattta cggaaattac ttcccgtagt ttataaaatc ttgccttatt gccgataggc
     9541 tgtcttttga acactattta ttcggaaaaa ggggctatga catgcttttt gtcatgcctt
     9601 ctttttatgt gggctgcttt tttagatagg agaaaataaa aataactatt taaacccgaa
     9661 caaaaaggaa taatgcatta aaaatcatta gattttacaa aaaaataagt aaaccacgtt
     9721 gtcagtttca gaaagtagtg ttagactgtt ttcaatattt gaaacaacat tttttattca
     9781 aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt acactattat
     9841 tagttttaca tacctactta tatatcgtca taatatggat gacagtttct agccagtacc
     9901 gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct tatgataaag
     9961 tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc ggttatttcg
    10021 gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg tattcaaaaa
    10081 gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac tcaccaagta
    10141 gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa agaacaagga
    10201 atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt tgctgcaaga
    10261 gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat ggatgaagaa
    10321 ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat ttctatttct
    10381 cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt ggcaaacgga
    10441 caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt agaaggaatc
    10501 gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga aatgggattg
    10561 agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga gtttcttgag
    10621 gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt ttagggctgc
    10681 agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc ggtacaggac
    10741 taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt atgtgtggat
    10801 tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg cctgtcgttt
    10861 taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat gaaggagtag
    10921 gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt tctggtgtct
    10981 tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc accgttattg
    11041 gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca acagcagctg
    11101 attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta attgtaggcg
    11161 tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt ttagtcggag
    11221 gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga gatgcgccgt
    11281 tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta tggtcgattt
    11341 tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta tattttgcac
    11401 ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga tatcgtgcag
    11461 aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact ggcttttctc
    11521 aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc tatttctctg
    11581 cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca caaatcatcc
    11641 ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca gtccaaggaa
    11701 tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt gttgctgttt
    11761 ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa atgccgcaaa
    11821 cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct atcatattga
    11881 atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc ttcaagaagt
    11941 aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag agaaacaaac
    12001 ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg tcagctgatt
    12061 gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa
    12121 tttcaaaaaa aagcttctca tgtttgttga atattgttcc ttgtgaccga ggagttggca
    12181 ggagaacacc gtttatccat aaaaaggaat gtgacctaac ttttgtcaca ttcctttttt
    12241 ttatctttaa taaacacgaa caataaaatg tttttctttg ttaacattcg aaatttcttt
    12301 gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa aactgggagg aaaaacaatg
    12361 acagcaaaaa tctctgtgat tatggggagt acttcagatt gggaaacaat gaaagaagca
    12421 tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc tgctcatcga
    12481 accccagatt tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat caaaatcatt
    12541 attgcaggag cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa aacaactttg
    12601 ccggttatcg gtgtaccggt ccaatcacga acattaaatg gtttggattc gcttctttcg
    12661 attgtccaaa tgcctggagg agtacctgta gctactacag caatcggaaa agcgggagca
    12721 attaatgctg gactacttgc gatacagatg ctttccatgt atgatttaga attggaggct
    12781 aaattggctg aaagacgttc aatgttggct aaaactgtta tagaaagtag tgatcaactt
    12841 ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc aattaggtcg
    12901 tatgatggcg attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg atcctgtgag
    12961 cgattgtcca gccgcacaag ttgcagattg gcacattgaa agtgtctatg atgacacctt
    13021 tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc tatgaattcg aaaacgtcag
    13081 tgtagaggcg ttaaatgcga ttttacccat gtcatttatt cctcaaggga cagacttgct
    13141 tgcgattacg caagaccgcc tgttagaaaa atcttttttg gaaacgaata acatcgtgat
    13201 tgctccttat gcaacgatcg tcagtccgac ggatatccaa gatgcgattg acgggatcgg
    13261 ctatccttgt gttttgaaaa ccactcgagg cggctatgat ggcaaggggc agtatgtttt
    13321 gaaaagtcgg gcggatctag ctcctgccat ggacctttta agagaaggaa cctgtgtttt
    13381 agaagcatgg atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa atggtcaagg
    13441 agatttcacg acttttccgg tcgtggaaaa catccaccac aacaatattc ttcatgaaac
    13501 gattgctcca gccgcgattg atcaggatgt gatcgaggag gctgagcgta ttgcccgtgt
    13561 gattgcagaa gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt taacaaaaac
    13621 aggcgggcta tatgtgaatg aattagctcc tcgtcctcac aattctggac attattctat
    13681 tgaagcttgt tctatgagtc agttcgatgc ccatattcga ggtatttgtg gttggccatt
    13741 aggagaaaaa gtacgtttga taagcaatgc agtaatggtc aatgttttgg gagaacaact
    13801 gctggaaagc tatcgcttga ttgagaataa gccagactgg caattccatt tctatggaaa
    13861 aaaacaagca aaaaaaggtc gcaagatggg acatatcact atcttgacag aagacatcca
    13921 gcaaacgctg gaagaaatca aacaaacgaa tatttgggat tagaggagaa aaaacatgtt
    13981 agaacgttat acacgccctg aaatggggaa tatctggacg gaagagaaca aataccgtgc
    14041 gtggctcgaa gtcgagattt tagcagatga agcttgggca gaacttggtg aaatcccgca
    14101 agaagatgtg aaaaaaattc gtgagcatgc atcatttgat atcgatcgga tcttggaaat
    14161 cgaaaaatta acacgccatg atgtcgttgc gtttacacga gcggtttctg aaacattggg
    14221 agaagaaaga aaatgggtcc attatggtct aacaagtacg gatgtcgtag atactgctta
    14281 tggctatctc atcaagcaag caaatgatat tttacgggaa gacttgaaac gttttacgga
    14341 aatcattggc gaaaaagcaa aagaacacaa atatacagtg atgatgggtc ggacacacgg
    14401 tgtccatgct gaaccaacta cttttggatt gaaacttgcc acatggtatt cagaaatgaa
    14461 acggaacatc gaacgatttg aacatgcggc aaaaggagta gaagcaggaa aaatctcagg
    14521 agcagtggga acgttcgcaa atatcccgcc atttattgaa gagtatgtat gtaaacaatt
    14581 ggggatccgt ccacaagaaa tttctactca agttctccct cgggatctac atgctgaata
    14641 tttctcagcg atggcgctta ttgctacaag tattgaacgt tttgcaacag agatacgcgg
    14701 attgcaaaaa tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc aaaaaggttc
    14761 ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa aatatggctg gtctagccag
    14821 agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc acattatggc atgaaagaga
    14881 tatttctcat tcttcagcag aacgaatcat cttgcctgat accacgattt tattgaatta
    14941 catgttaaat cgttttggca atatcgttaa aaatctaacg gtctttcctg aaaacatgaa
    15001 gcggaatatg tctgcaacat ttggtttgat ttatagccag cgcgttatgc tgaaattgat
    15061 cgataaggga atgacaagag aagaagctta tgatcttgtt cagccaaaaa ctgcgtacgc
    15121 ttgggacaac caagtgcagt tccgcccgct gttagaagaa gatccaacca tcacatcggt
    15181 attatctaaa gaagaaatag acgatgcctt tgattatcat catcatttga aaaacgttga
    15241 cgtgatattc aaacgagtag gtttggaata aagaagagtg agttgcaaaa ttcaattaaa
    15301 atagaatagc aaaaataacc aaaaaagaag cagatccgtt agcggttctg cttcttttag
    15361 tttattatat tttcttatca ataataggaa cctgatttta aaagcggcta cctctttacc
    15421 gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa atggcttggc tattataact
    15481 aaaatgctgg ttatggagat gttgcagcaa gttttcgaga aaagcgtatt ccatctcttg
    15541 tccattgaat aagatctgga tacgataatg atttttgaaa cgcttctgtt catgaatctg
    15601 actgattgaa agttcgcctt tgtgttctgt ctgataaaga aaagtccttt tacggcattc
    15661 agcacagcgc ttggttgctc ggcggatttt tcggaatgta taaaaactag tatctttatt
    15721 gcataaataa acgtctttga tcgacagcaa atcattatat agacaggaac gttcttcagc
    15781 tgttaattct gataaaaaaa gctgaccttg ttttattgca tcttccatcg gcaagtgcct
    15841 cctcgaaccc attatccaaa tttttagtta attctaatat accatcatta agtataaatg
    15901 taaacgcttg ttgatggtat aagattttcg tttctgataa aacacgaaca ttaaaagaat
    15961 ctaatgcctt aaatgtacat atttttgttg atttataaaa agatgattgc taaaatttaa
    16021 agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat gtaaataaaa aggagagacg
    16081 tacatggaga agaaagaact tttgtatgaa ggaaaagcca aaaaaatata tcttacttct
    16141 gatgagaact tgatttggat agaatattta gatcaagcaa ctgcgttgaa tggcgcgagg
    16201 aaagaccaga taagtggaaa aggagaattg aataatcaaa tcacaagtgc aattttccgt
    16261 tatctgactc aaagaggaat aaaaaatcat tttattgaac aattgtctaa aaccgaacaa
    16321 ttagcaaaaa aagtacaaat tatccctctg gaagtggtta tccgaaatat tgcagcagga
    16381 agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat tcatcaaacc aatcattgaa
    16441 ttctactata aggatgacca gttggatgat ccatttatca atgaagagca tatctatttc
    16501 ttacaacttg caaagccgga agaaatcagt gagctgaaag cccaagcgtt ggctattaat
    16561 gaaattttga ccgaattatt tgctaaggtc ggtttggttt tagtagattt caagctagaa
    16621 tttggccggt cgaatggaca aattttgctt gcggatgaag taacaccgga tacttgccga
    16681 ttatgggatg aacgtacgaa tgaacatatg gacaaagacg tttaccgcag agatctaggt
    16741 gatttgatcc cagtctatca agaagtatat aaccgtctat cggaactaac tgaagaaaag
    16801 gagcgagtat gatgtatttc gtaaaagtat atgtaaccta taaagattca gtgttagatc
    16861 ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg atatgaaaca atcgaagata
    16921 tccgaatagg aaaatacttt gagatacaac ttgctgatgc agaagaacca atcgatacag
    16981 tgattgaaac gatttgtgac aagctgcttg caaatgtaaa tatggaaaca taccgatatg
    17041 aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg tcatcgtttt tccaggttca
    17101 aattgtgacg cagatatgtt atgggcagtt cgcgagatca tgggcgctga tgccgaattt
    17161 gtacggcatg atgagtcctc tttagaagga tttgatgggg ttttactgcc gggaggattc
    17221 tcctatggtg actatttgag atgcggtgcg attgctcggt tttctacaat catggaagaa
    17281 gtgattcgtt ttgctgaaga agggaaacca gtattcggta catgcaatgg attccagata
    17341 ttgacagaag caggactttt gccaggtgta ctgagacgaa atgagtcttt gcattttatt
    17401 tgcaaaactg taccattgaa agtcaacccc aagacggcct ttacttcaga gtatgaagcc
    17461 gacgaaataa tctatcttcc agttgctcat ggggaaggaa attatttctg tgacgaagaa
    17521 acacttcaag aactaaaaaa aaataatcag attgccttta cttataatga caacatcaac
    17581 ggcagcgtgg aaaacattgc agggattacg aacaaagcag ggaatgtgtt aggaatgatg
    17641 cctcatccag aacgtgcagt agaagacttg ctaggatcag aagatgggaa acggttcttt
    17701 gcttccattt taaaaaactt cggaaaggtg accgtataga tgaaaacatt gaaagaaccg
    17761 acagctgaag agatcaagaa aaaaagaatc tatgccgaat ggggattgac tgatgaagaa
    17821 tatcgaatga tcgaagaaga tatcttagga agaatgccta attatacgga aactggactg
    17881 ttttctgtta tgtggagcga acattgttcc tataaaaatt ctaaaccggt tctacgcaaa
    17941 tttcctacta gtggacctca tgtgttgcaa ggccctggag aaggcgcagg catcgtcgac
    18001 atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc acaatcatcc ttcagccgtg
    18061 gagccttatg aaggagctgc gacaggagtt ggcggaatca tcagagatat tttcagcatg
    18121 ggggctcgtc caattgcgtt gctagattca ttgaggttcg gcgaattaga taatccaaga
    18181 acgaaatatt tactagaaga agtagttgca ggtatcggcg gatacggcaa ttgtatcggc
    18241 ataccgactg taggcggcga aattgcattt gatccttgtt atgaaggaaa cccattagtc
    18301 aatgcaatgt gcgtaggttt gattgaacac aaacatatcc agaaagggca agccgctggc
    18361 gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc gcgacggtat ccacggagca
    18421 acttttgcct cagaagaatt taatcaagaa gaagaacagc aacgttctgc tgtacaagta
    18481 ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt tggaattaat cttgcatcat
    18541 tcggattctc taataggtat acaggatatg ggagcagcag gacttgtttc atctagtgcg
    18601 gaaatggctt caaaagctgg ctcaggactt atattgacat tagatgaagt cccacaaaga
    18661 gaaactggga tgacacctta tgaaatgatg ttatcggaat cacaagaacg gatgttgatt
    18721 tgtgttaaat caggagagga agaaaaaatc caagaactat ttcagaagta tgatctagat
    18781 gcagtcacta tcggtaaagt aacagacgac gggcaatatc ggctatatca tcaaggagaa
    18841 gaagtagcaa atctgccagt cgatgcatta gctgaagatg caccagttta tcataaagaa
    18901 atgaaagaac cagctcgaat cgtagaattt cagcagttag ctccttatca gccagttatc
    18961 gaagaacctg gacaagtatt gcttgatctg ctgcagcaac cgacgattgc ttcaaaacgt
    19021 tcaatctacg aaacttatga ttcacaagta caaaccaata cagttgtccg tccaggaagc
    19081 gatgctgctg tgatgagggt tagagggacg aataaagcgt tagctatgac cacagactgc
    19141 aacgcgcgct atctgtattt ggatccaaaa atcggaggac aaatagcagt ggcagaagct
    19201 gctagaaata ttattgctag cggtggtaag cccttggcga tcaccgactg cctgaactat
    19261 ggttctcctg ataaaccaga agttttttgg gaattatcga cttcagctga cggaattgct
    19321 gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa atgtctcttt atataatgaa
    19381 acggatggtc aagcaatcta tccaacacca atgattggta tggtcggatt gattgaggat
    19441 cataaacata tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt
    19501 aagacgtttg cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa
    19561 ggtgtgatcc gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca
    19621 gccatccaaa acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt
    19681 gcactggcag aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc
    19741 tctgctcaat tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat
    19801 aagacacgat tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat
    19861 gaagcgataa tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca
    19921 agaaagtgct gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat
    19981 gcgggatctt cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc
    20041 atagtcttca gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc
    20101 ttaaggggca tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc
    20161 agcagttaac aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa
    20221 gtgtggacaa tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc
    20281 ataatggtaa cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga
    20341 tcttccattc taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa
    20401 cagatttttt aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt
    20461 tattaatgac agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag
    20521 caatcggaca aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag
    20581 ttattggggc acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg
    20641 aaggaatcca gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat
    20701 atatctattt tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga
    20761 aaaatatggg acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg
    20821 tacctaattc ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg
    20881 aaatcggatt agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc
    20941 taagagagca aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa
    21001 aggtgattat ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc
    21061 ttttgaaaga agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat
    21121 atccatgttt ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca
    21181 cgattgatga aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg
    21241 gtctgatcga atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt
    21301 atttcaacgg agattatccg acgccgttat atgactatga agaaaaatat caggaatcac
    21361 taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg
    21421 gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg
    21481 atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc
    21541 ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc
    21601 gatggcgtgg ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg
    21661 atcgactgcg tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat
    21721 ttcttagact acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa
    21781 ggtgtagcag atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa
    21841 atgcctggta tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct
    21901 gaaaaatctc aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata
    21961 gcatctagcg ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag
    22021 cagcagtttt ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg
    22081 cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata
    22141 aaaggagctg cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca
    22201 gaatctatgg ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt
    22261 ttacaaaaat atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc
    22321 ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa
    22381 gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg
    22441 tttataggag aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc
    22501 aagcgctagc cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt
    22561 gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt
    22621 gtttttcacc taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat
    22681 tgaaagaaaa aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg
    22741 tattgctgaa aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct
    22801 tccctggttt gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca
    22861 caattcatta tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga
    22921 gaattgaaca ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc
    22981 gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag
    23041 agcattgatc agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga
    23101 acaaggagta gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat
    23161 caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa
    23221 aacacttcat cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat
    23281 ggaggcaatg gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc
    23341 tttcaaagaa acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga
    23401 tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt
    23461 tgttgatcca tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc
    23521 actttctttc agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc
    23581 actgatcgct caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact
    23641 tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt
    23701 ttatcagtcc gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa
    23761 agaactttct tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata
    23821 tgacgagcca acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc
    23881 tacgatttta gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg
    23941 gatcatcgtt ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt
    24001 tctagaaatc atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa
    24061 gaaaaacatc cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga
    24121 aacggtctca gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga
    24181 agaagagtgg aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagt
    24241 gtttgcttgg agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg
    24301 tcagacagta ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat
    24361 tgaacaagcg caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat
    24421 ggatgattcg gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg
    24481 aagcatcaaa gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt
    24541 tactgatgtt cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat
    24601 cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga
    24661 tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc
    24721 tatcagtgag cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg
    24781 gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga
    24841 aggattgaaa atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt
    24901 tgctaaacaa ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg
    24961 ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga
    25021 cggcttagca gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc
    25081 ggcagaacaa atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga
    25141 agagtttttg gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta
    25201 tccgatggtc atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac
    25261 aggcggaatg ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc
    25321 tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac
    25381 gggtgtttta tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa
    25441 tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc
    25501 gcaagtaatc gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg
    25561 atacaatgta ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat
    25621 ggttctacca gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa
    25681 taacggcaaa ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac
    25741 gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac
    25801 attttatcgt acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat
    25861 cagctgcttc catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa
    25921 gccttttcat aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga
    25981 tgaaaaaaat tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta
    26041 tgcgtatcaa caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca
    26101 ttgatttttt tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag
    26161 atgctctagc aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa
    26221 tcgttccagg tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa
    26281 gtttgacccg tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat
    26341 tggtagaacc agaggaagta gctgaagcgt ttgatgaatt ggaacggtca ggaaaagttc
    26401 gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc
    26461 gtcaaccatt agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc
    26521 aagggattca tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg
    26581 attattcccg attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt
    26641 ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac
    26701 tagcagctaa atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc
    26761 cagccaatat gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc
    26821 aagcttcaga aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa
    26881 acattttacc ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga
    26941 ataacttgaa agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa
    27001 gagtatcatt gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc
    27061 cgatattctt ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg
    27121 tcattataac caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat
    27181 ttttttgcta attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat
    27241 tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa cttgttgagc gacccaagtt
    27301 aaggacatat ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt
    27361 ggctgatatt tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata
    27421 taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat
    27481 gtgcaatgaa ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc
    27541 ttagtcaaga caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc
    27601 aagtgttcga tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg
    27661 tcaaagtaaa agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg
    27721 ataatttgtt ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat
    27781 gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt
    27841 tgtccctttt ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca
    27901 ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag
    27961 atcacttgtt gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct
    28021 tcaaagtctg tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa
    28081 gagcgattga aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac
    28141 aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt
    28201 tctttttgct gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta
    28261 cttttaatcg gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa
    28321 tcgaaatcgt cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct
    28381 tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg
    28441 ggctgtaagt cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa
    28501 gtgattggat aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg
    28561 tattcgtctt cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga
    28621 tagagggatc atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg
    28681 tttttgatca tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc
    28741 gatactgaaa tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc
    28801 tgcagggatg ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca
    28861 tgtggtaata cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt
    28921 ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat
    28981 atcgatctta tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt
    29041 gatatggcta atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg
    29101 aaaaaagccg gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat
    29161 caaagaaata atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa
    29221 tacacgaact tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg
    29281 gtttagatct tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc
    29341 cggcagggtt aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag
    29401 ctgattgatc ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa
    29461 aaaaatggta ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag
    29521 tgtcgtattt tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc
    29581 tgacagcagt tgagtgacag aatctacctg attgatgatc tcttcattgg tatccataaa
    29641 atcttttgcc aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt
    29701 acgtagcgat acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc
    29761 tcgaatatca ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac
    29821 ttggccagaa atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat
    29881 tgcatctgct ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata
    29941 taacagtatg ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg
    30001 ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc
    30061 tggttgggat gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt
    30121 taggtaatgt ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat
    30181 accagccgac atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct
    30241 cgatcatatc ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg
    30301 atgtttcttt tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata
    30361 catcgatttc ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca
    30421 tccgaataaa atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg
    30481 ttccatcttt ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta
    30541 gcttggtcgg tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct
    30601 ttgaatttgt gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac
    30661 tggggccaat gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa
    30721 gtatgacagg gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt
    30781 gcaaaggagt aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga
    30841 tttgccgttc ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa
    30901 gcgcagtgat aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg
    30961 aaaaaccaac gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt
    31021 ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca
    31081 tctggatttg ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca
    31141 atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat
    31201 ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg
    31261 tatcaattga aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat
    31321 cgctattttg accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga
    31381 tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga
    31441 tgggaacaac atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg
    31501 agaagataaa gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga
    31561 tacacttgtg attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa
    31621 agaagcagat atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga
    31681 ttattacgcg acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga
    31741 gcacttagga ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc
    31801 tgacgacaac aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat
    31861 ggacaacggt cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct
    31921 tcgctgggat ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac
    31981 tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc
    32041 gtcattaaaa ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca
    32101 agatgcgaca gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt
    32161 taaagacaca cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg
    32221 agaagaggtt ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac
    32281 gtatttagca aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac
    32341 cgattattat aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag
    32401 atctaagtca gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc
    32461 accaaaaaat tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg
    32521 gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg
    32581 agcggcttgt atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt
    32641 caatttaaag atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta
    32701 gcactaagtc cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa
    32761 agaggcgtga ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc
    32821 ggtttgtcta tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta
    32881 gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc
    32941 gctgcattga atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag
    33001 caaggtgtaa cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat
    33061 cgaatcacca ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc
    33121 gaagagcata ttattcgcgg aatggtgggc agagatttag ttgatcgtta tcctgaaaga
    33181 aaaccggata ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt
    33241 gatcacgagc gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta
    33301 ggaatagctg gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat
    33361 tcttatggaa gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag
    33421 accgttcctt cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta
    33481 ggactgaatc tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata
    33541 agtcaaaaag gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa
    33601 aagatgcgta taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat
    33661 caacaaaaag tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat
    33721 gagccaaccc gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa
    33781 atggcagcaa atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg
    33841 atgtgtgatc gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa
    33901 gatgcagatc aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga
    33961 acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc
    34021 tgatcttttt ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca
    34081 attgatgacg gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa
    34141 tagccatata ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga
    34201 tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa
    34261 tcgccaaatg aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg
    34321 cgcatggcaa ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc
    34381 aggattgctg atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc
    34441 ttttccagct gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag
    34501 cagccttcac ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg
    34561 tcaatggcga acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc
    34621 tttcttggta aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc
    34681 ctcttatcaa gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg
    34741 gtttttgaca aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa
    34801 agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat
    34861 gggagtcatg gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc
    34921 tcaagcaggt acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc
    34981 gacttccggc ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt
    35041 attgaacaat ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg
    35101 attgatcttg cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta
    35161 ggagaacgcc ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt
    35221 cgctaaaagc gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt
    35281 accattcgta taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca
    35341 tagggcgcta aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac
    35401 atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata
    35461 aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat
    35521 tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat
    35581 tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc
    35641 cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga
    35701 aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca
    35761 ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa
    35821 aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg
    35881 aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta
    35941 ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct cttagatggt
    36001 gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata
    36061 ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc
    36121 acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg
    36181 gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga
    36241 tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat
    36301 ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg
    36361 tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt
    36421 gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat
    36481 gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat
    36541 aagggactgt gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt
    36601 ttttctcgta aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat
    36661 gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat
    36721 gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact
    36781 agggcaactt tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga
    36841 gcttggggta ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg
    36901 gaaattcgct gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac
    36961 ggactggtat gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga
    37021 tttatacgct agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc
    37081 tcttgaagaa atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg
    37141 taacaaaatc aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct
    37201 tcttgtgact ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc
    37261 tggagagggg atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc
    37321 ttcaggaacg cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact
    37381 gaaagaatcg atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa
    37441 ccctacagga aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca
    37501 gtatcggctt ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga
    37561 agaagtcctt cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc
    37621 tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc
    37681 gcaactagct caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac
    37741 agtggctaat gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt
    37801 gaaagaaaaa agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca
    37861 ttcaatttta ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga
    37921 actagcagaa aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca
    37981 agatataaat ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt
    38041 ttattcaaag atgaaaattc tagaaaagtg gttaacagaa taatttcaca aagtaaatag
    38101 attaaagaat ataacagctt ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag
    38161 aatacgatat aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa
    38221 ataaaataaa tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg
    38281 gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat
    38341 tgtgatttta ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga
    38401 aaatttgtgc ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg
    38461 ttaaatagtg atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata
    38521 gatgagagag gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac
    38581 tgctagatga agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg
    38641 tagttaacaa agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc
    38701 atatggtatg gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc
    38761 taggcttttt tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat
    38821 ttgataaaga agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg
    38881 gtttaccttt atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc
    38941 cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag
    39001 ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg
    39061 gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc
    39121 atgcaaatgc cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa
    39181 aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc
    39241 aagtcgatgg aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg
    39301 cgatcagtgg aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata
    39361 cactatctgg tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga
    39421 aaaaagatcc attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag
    39481 aaaaagaaga acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag
    39541 ccgacaaagt accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc
    39601 cacaatcaat caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc
    39661 acaaaaaaca atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga
    39721 gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga
    39781 aggattgcag gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg
    39841 aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca
    39901 attctttggt tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag
    39961 atatcgaatg agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg
    40021 tgttcataca ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg
    40081 tattcgagta gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat
    40141 tagaagtaac gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga
    40201 agaagtgccg gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg
    40261 aacagatgta tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga
    40321 caatcttgca aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt
    40381 agatgtagaa acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga
    40441 agcacaacga caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat
    40501 cctttcatta gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg
    40561 acaagcagaa aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat
    40621 cgcggaattt taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaatt
//
LOCUS       DACEKQ010000013        39464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEKQ010000013 DACEKQ010000000
VERSION     DACEKQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.013"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            155..2344
                     /locus_tag="IW671_000628"
     CDS             155..2344
                     /locus_tag="IW671_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0897639.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..4134
                     /locus_tag="IW671_000629"
     CDS             2341..4134
                     /locus_tag="IW671_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0897640.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4334..4648
                     /locus_tag="IW671_000630"
     CDS             4334..4648
                     /locus_tag="IW671_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0897641.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4669..5046
                     /locus_tag="IW671_000631"
     CDS             4669..5046
                     /locus_tag="IW671_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0897642.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            5056..5829
                     /locus_tag="IW671_000632"
     CDS             5056..5829
                     /locus_tag="IW671_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897643.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5851..7254
                     /locus_tag="IW671_000633"
     CDS             5851..7254
                     /locus_tag="IW671_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0897644.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7436..8620
                     /locus_tag="IW671_000634"
     CDS             7436..8620
                     /locus_tag="IW671_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0897645.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8617..8907
                     /locus_tag="IW671_000635"
     CDS             8617..8907
                     /locus_tag="IW671_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897646.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8904..9125
                     /locus_tag="IW671_000636"
     CDS             8904..9125
                     /locus_tag="IW671_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897647.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9167..9946
                     /locus_tag="IW671_000637"
     CDS             9167..9946
                     /locus_tag="IW671_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0897648.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            10013..10507
                     /locus_tag="IW671_000638"
     CDS             10013..10507
                     /locus_tag="IW671_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0897649.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            10563..11201
                     /locus_tag="IW671_000639"
     CDS             10563..11201
                     /locus_tag="IW671_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897650.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            11273..11668
                     /locus_tag="IW671_000640"
     CDS             11273..11668
                     /locus_tag="IW671_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897651.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            11652..14105
                     /locus_tag="IW671_000641"
     CDS             11652..14105
                     /locus_tag="IW671_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0897652.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            14102..16129
                     /locus_tag="IW671_000642"
     CDS             14102..16129
                     /locus_tag="IW671_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897653.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            16126..17148
                     /locus_tag="IW671_000643"
     CDS             16126..17148
                     /locus_tag="IW671_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0897654.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            17165..18094
                     /locus_tag="IW671_000644"
     CDS             17165..18094
                     /locus_tag="IW671_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897655.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            18339..18455
                     /locus_tag="IW671_000645"
     CDS             18339..18455
                     /locus_tag="IW671_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0897656.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            18471..20390
                     /gene="tet(M)"
                     /locus_tag="IW671_000646"
     CDS             18471..20390
                     /gene="tet(M)"
                     /locus_tag="IW671_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0897657.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            20488..20676
                     /locus_tag="IW671_000647"
     CDS             20488..20676
                     /locus_tag="IW671_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897658.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20734..21087)
                     /locus_tag="IW671_000648"
     CDS             complement(20734..21087)
                     /locus_tag="IW671_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897659.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21618..22040
                     /locus_tag="IW671_000649"
     CDS             21618..22040
                     /locus_tag="IW671_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0897660.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            22037..22267
                     /locus_tag="IW671_000650"
     CDS             22037..22267
                     /locus_tag="IW671_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897661.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22765..22965
                     /locus_tag="IW671_000651"
     CDS             22765..22965
                     /locus_tag="IW671_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0897662.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22992..24185
                     /locus_tag="IW671_000652"
     CDS             22992..24185
                     /locus_tag="IW671_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897663.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(24253..25818)
                     /gene="guaA"
                     /locus_tag="IW671_000653"
     CDS             complement(24253..25818)
                     /gene="guaA"
                     /locus_tag="IW671_000653"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0897664.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25877..26800)
                     /locus_tag="IW671_000654"
     CDS             complement(25877..26800)
                     /locus_tag="IW671_000654"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0897665.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26996..27601
                     /locus_tag="IW671_000655"
     CDS             26996..27601
                     /locus_tag="IW671_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0897666.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27909..28925
                     /locus_tag="IW671_000656"
     CDS             27909..28925
                     /locus_tag="IW671_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0897667.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            29066..30367
                     /locus_tag="IW671_000657"
     CDS             29066..30367
                     /locus_tag="IW671_000657"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0897668.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30413..31075
                     /gene="deoC"
                     /locus_tag="IW671_000658"
     CDS             30413..31075
                     /gene="deoC"
                     /locus_tag="IW671_000658"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0897669.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            31096..31494
                     /locus_tag="IW671_000659"
     CDS             31096..31494
                     /locus_tag="IW671_000659"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0897670.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31560..32642
                     /locus_tag="IW671_000660"
     CDS             31560..32642
                     /locus_tag="IW671_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897671.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32854..34416
                     /locus_tag="IW671_000661"
     CDS             32854..34416
                     /locus_tag="IW671_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897672.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34409..35542
                     /locus_tag="IW671_000662"
     CDS             34409..35542
                     /locus_tag="IW671_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897673.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35542..36498
                     /locus_tag="IW671_000663"
     CDS             35542..36498
                     /locus_tag="IW671_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897674.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36597..37763
                     /gene="deoB"
                     /locus_tag="IW671_000664"
     CDS             36597..37763
                     /gene="deoB"
                     /locus_tag="IW671_000664"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0897675.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37787..38605
                     /locus_tag="IW671_000665"
     CDS             37787..38605
                     /locus_tag="IW671_000665"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0897676.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38622..39329
                     /gene="deoD"
                     /locus_tag="IW671_000666"
     CDS             38622..39329
                     /gene="deoD"
                     /locus_tag="IW671_000666"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0897677.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        13171 a         6704 c         8039 g        11550 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg aatagaagtt
     3601 aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa aagaatgtat
     3661 agtggagacg acaacggtgt tgatttatac aaatttgtta ccaaacatat atttgttaat
     3721 cttaatattg acgagaaaaa ttacccgtct atttttaagc actatctatt ttttattaac
     3781 gaagtagaag atcggataaa aaataattca ttatctagta gtgtatctag ttttaagaag
     3841 atgtttaaat ctcgagatgg tatcacactt actacagcac ataaagttaa aggaaatgag
     3901 tatgatactg ttatcgctat taatcttcta aacgggaaaa ttcctcattg gaatgaaatt
     3961 tttaatgttt ctgataaagg aattagttca gcgagaaaac tactatacgt aatttgttca
     4021 agagcaaaaa ataatttatt tcttttctca gaggtgggtt attttacgag taaaggagac
     4081 ttgctttatc ccactcaatt attagataaa atagaatttg attataatat ttaaaatacc
     4141 taggtagtcg ttgttttaaa tatagtagga atcggacgct cagtacggag cgtctatttt
     4201 tttacccaat tttgaaagga agtgaactta tgaaaataaa aaatcaagaa tcaaaaggtc
     4261 gctctccccc ctttaaaacc atcacacaaa taagattcat tcaaacataa aaacagaaag
     4321 gataggtaaa aatatggaac ttaaatatgt cattcccaat atggaaaaaa catttggaca
     4381 attagaatat gctggtgaag ggaacattga acaacgtcga ataaatggca gaccaaccat
     4441 tctctctcgt agttacaatt tatactcaac gattcaacga gcagatgata ttgttgtcat
     4501 tcttccctcc gaagctggcg agaaacattt tgaatccgac gaaaaagtca aactcatcaa
     4561 cccacatatt accgcagaag gttataaaat tggcaatcga ggatttacca attacattat
     4621 gcacgcagac gatatggtga aagcataaga aaggaaaggt gaaaaaatat gagattagca
     4681 gaaggtattg tgattgaaaa agaccagaca tttggcacat tgaaattttc cgccttgcgt
     4741 cgtgaggttc gtatccagaa tgaagatggt acggtttccg aagaaattaa agaacgtacc
     4801 tatgacttaa aatcaaaagg acaaggacgc atgatacaag tcagtatccc cgcttctgtt
     4861 cctttgaaag aatttgacta caacgcagaa gtggaattaa tcaatcctgt ggcagatacc
     4921 gtagcgacgg ctacttttcg aggggcagac gttgattggt acgtgaaagc agacgatctt
     4981 gtattaaaag gacagacaaa acctcatcag aaatataaaa aagaagcgtc cgaaagtgaa
     5041 aaataggtgt tgcttatgac aaacacacca ttgattttga aagaaattcc gaaggatgaa
     5101 gccatttctt ttattcggca ataccattat tcaaagatac tccctagatt gtgtaaatac
     5161 tttctaggga ttttttcaga agaaaaatta ttaggcgttg tggaattagg ctggggtacg
     5221 caacccctac aaaccatacg caaactcttt cccgacagtt cccttcaaac cacagattat
     5281 ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta ttttggcagt
     5341 caagctcttt cagctctaat taagtggttg aaagaacata cggactgtca ttttttatat
     5401 acactggcag atggaattga gggaaaatgt ggctatgtct atcaagcgtc caacttcttt
     5461 tactgcgggt atttcaagac aagtgtctat cgtgataagc agtcatggga aaagatacac
     5521 cctcgaagtg ctcgcctttt attagaggaa aatgctcgct ttgaacaggt agagaaaaag
     5581 cattggcttt cccaggcatt ttgcgaatac aaagggattg agaaaatcaa tggacgcatg
     5641 tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca cactctctat
     5701 cgacgtcatt attatccgaa agaaaaaaat ttacgctttg aaaagcgaat cgcttatcag
     5761 aaatatgaag ccatttctca accaactttt gataaacagg ctcgtatcta caatacacaa
     5821 ttattttgat aggtggaaag gaggattttt atgccattgc ttcatgacag aggagagcga
     5881 atcaaagcca acgataagca cttggtctat cattttgtac taatttcatt gctggtagtt
     5941 tttctagtcg tcctactccc ttttcattgg agagcgttac aatcggtgaa ctggcaagca
     6001 gttcagttag atacattttt ctatcaactt catacgccat acttttggag cagtattctc
     6061 actgctcttt ttgtatgcgg acttatcagc tggctttatt atcactatcg tatagattct
     6121 ataaaacagt tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa tcattggtat
     6181 gaagccgaag aaactcaatc agagggattc tttaaagatt tacccagcaa gaaaaccaaa
     6241 caacgaatcc gccattttcc taaaatatat tatcgaatga aagatggact aatccatatt
     6301 catgtagaaa tcaccttagg gaaatatcaa gatcagcttt tgcacctaga aaacaaacta
     6361 gaaacaggat tgtattgtga attagtggaa aaaacgttga aagattctta tgtagagtat
     6421 gtcctacttt atgatactat tggcaatcga attggcattg aagacgtggt agctcaagat
     6481 ggacgtgtca aattgatgga atcggtctat tgggagttcg attctctccc tcacatgtta
     6541 attgctgggg gcactggtag tggaaaatcc tattttattc tcacattgat tgaatctctc
     6601 ctgcatacaa atgcaaagct ttatattctt gaccctaaaa atgctgattt ggcagactta
     6661 ggggcggtca tgaataatgt ctattaccgt aaagaagata tgttggcatg tatcaatcaa
     6721 ttttacgaag atatgattga tcgcagtgaa accatgaaac aacaccctaa ctataaaaca
     6781 ggagaaaatt atgcttatct tggtttgtct gccaattttc ttatttttga tgaatatgtg
     6841 gcaatgatgg atatgctagg tcgtgaaagt accaccgtta ttcataaact caaacaaatt
     6901 gtaatgttag gacgacaagc aggcttcttt ttaattcttg cctgtcaacg tccagacgca
     6961 aaatatttta gtgatgggat tcgggacaac tttaacttcc gtgtggcttt gggtcgtatg
     7021 tctgaaatgg gctttggtat gatgttcgga agcgatacac aaaaacaatt tttcttaaaa
     7081 cccatcaagg gaagaggtta cgtggataca ggaaaaagtg tgattagtga gttttataca
     7141 ccccttgtac caaaaaggta cgatttctta ggagaaatag gaaaagtcat tcaaaaaaag
     7201 caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct ttgattggtg
     7261 tggcgttagc cacgccaatc ctttagcccc ctctatctaa caggggggca caaatacaac
     7321 tgaaaacagg caattctcat caaccaatgt tgatggtaca aaggattgcg gaaaatatga
     7381 aaggtgtaaa ctcaagggtc atagtttaca tcttttttag attggaggtt tgcgaatgga
     7441 aaacgaaaaa atttggatta aggaattgaa agaaaagcga ctggcttatg gcgtttctca
     7501 aaataaactg gcggttgctt ctcatattac cagaccttac ctatcggata ttgaaacagg
     7561 caaggcagtc cctactcaac aggtgaaaga agatttactg aacgctttgg aacgcttcaa
     7621 tcccgataac ccattggaga tgttattcga ctatgtacgg attcgattcc ctaccaatga
     7681 tgtggctcaa attatcggtg aggttttacg tttgaatatg gattatatgc tacacgaaga
     7741 ctttggctat tattcttatc ccgaacacta tcgtttcggt gatattgttg tcctagtctc
     7801 tcatgatgta agtaaaggtg ttttattaga actgaaagga aaaggctgtc ggcaatttga
     7861 aaactttcta ttggctcaac atcggagctg gtatgatttc tttcaagatt gcatggaaca
     7921 taaaggtatt ttcaaacgct tggatttagc tatcaacgat aaaacaggga ttttgaatat
     7981 tccagatttg gcaaagaaat gtaaacagga agaatgcatt tccgtctttc gaagctttaa
     8041 aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg gaaacacact
     8101 ttatattggc tcattaaaaa gtgaagtcta tttctgtata tatgagaaag attatgagca
     8161 atacgtgaaa aatgatgttc ccttagaaga cgcagaagta aagaatcggt ttgaaattcg
     8221 gttaaaaaat gaccgagcca cccaagccat gaaagattta ttggcacatc aacaggcgga
     8281 aaaaacagcc tttgaaatta tcaatcgcta tatccgcttt gcagataaag acgacactaa
     8341 acgtcggagt gattggaaaa ctaatgaacg ttgggaatgg tttatcggta aaaatcgtgg
     8401 agcattacgc ctaaccacac agccagagcc ttattctttt gaacgaacat tgaactggtt
     8461 acatcatcaa gtcgccccta cattaaaaat cgcaagcatt ttagatgttc tcaacggtac
     8521 aaccattatc tccaccatga ttcaggaagc caaactgaca gaaaaacatg agaaattaat
     8581 tgaacaacaa catctagcca tggaggattt gattacatga gccttttaac aaaagaatta
     8641 aagaagttag ggtttcagtg tggcattgag tttcaagcgt atatacaaaa tacagggaaa
     8701 tatacgtctc tcattattga gggaaaacgg caagctggtg ataccatcta tacctatgat
     8761 ttttacaagg tgagatttta taataattat accaatcgag taaccgtcta tggagagcat
     8821 ttaacccctt ttcaattatt aaggcgtgta aaaagctata tctattatcg agaaaaatat
     8881 cttaaggaaa ggaggacaat cacatgaatt ttgggcagaa tttgtacaac tggttcttgg
     8941 ataatgcaca gtcattggta ttgttagcga ttgttgtcat tggactgttt ttaggtttta
     9001 aacgagaatt ttcaaaactc attggcttct tgattattgc tctaatcgcc gtcggacttg
     9061 tgttcaatgc agctggcgtg aaagatgtgt tattaaactt atttaaccga atcattggtg
     9121 cttaacagat atttttcatg ataaaatagg aagtatatat tttgttatga aaggatttat
     9181 gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag
     9241 ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt
     9301 aactttagat gagactgacg attttactta ttataaagga tataaaaagg gctggaatac
     9361 gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca
     9421 taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt
     9481 agtggatact attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt
     9541 gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg
     9601 tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat
     9661 actaaaaaaa tatgaaatta cagttcctag agacgaaaat ctatctaaat tatatagtag
     9721 gatacaacaa ttaattgaaa aagaaattca acctactgaa cttgctgata tagtgaagac
     9781 aacgataagg tcatcaaatg gaatgattag ctctcttaat gaagcaagaa ataggcattc
     9841 acttgcacac cctaatacaa atattattgg aaagagagaa gcaaaactaa taataggtat
     9901 ttcaagtaca gttacagatt atattagcgg ataccttgat aagtagcgtc attaactgat
     9961 tgctactttt tttatgacta tttttataaa aaggatgtga taagaattgg aaatgaaagt
    10021 ctatgtggca aatttaggac ggtataatga aggtgaatta gtcggagcat ggtttactcc
    10081 accaattgat gaggaagaaa tggcagaaag aatcggttta aatgaggact acgaagaata
    10141 tgcaatacat gattttgagc ttccttttga tgtggacgaa tacacgccga ttagtgaaat
    10201 caaccgtcta tgtgaagcca ttcaagaaat cgaaggtaca cctatttata atgaactcaa
    10261 agaaattcaa ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca aagaggatat
    10321 tcattgttat agtgattgtg attcgatgga agatgtcgcc cgttattacg tggaagaaac
    10381 aggacagctt ggtgaagtcc cttccaattt acaaaattat attgactacc aatctttggg
    10441 acgtgatatg gaaatagagg gtaattacct agttacctct catggggtat ttgaatattg
    10501 tcagtaagtt taacaatccg tgttataatt aaattaataa atagcacgga gaggtataaa
    10561 ttatggtaaa tgtatttgaa aatcttaaac tattaaaact agacatgaaa gataaaggct
    10621 gggtgataga ttccttttac tttcgttaca aacagcaaaa ttatattgta cttgttaagc
    10681 tatttgaaaa agaggaaaaa gttccagaat atgctctgtt aaaactagaa tttttaaagg
    10741 aaaacgattt ttcagatatg ctagctgtct atgctaattc ggtaaaatta tatacagaca
    10801 cgaaaacaat aagagaatat tttggtatcg aatacagtag taacttggga gatgttttat
    10861 ttcaatttag ccaaactctt gctcgattta ttccgacaga agttagtgag aagaaaaatg
    10921 aagaccaaaa agaagccatg tgtttctcat taagtcaaag tgattcagaa gatcccagaa
    10981 aaaaatattg tttttcagtt agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac
    11041 gaagtccttt taatgacaat aaaacgagat tgtatagacc cagcttatat gaacgattag
    11101 ggtcagatac caatttaagt ttcagatatt caatgaatcc agatgatgaa gaaactgatg
    11161 aaggtattat tgcaaaatgg acaaaaaata aaattgaata gttaaagcat tgaaagagca
    11221 atcacctatt ttgattgttc tttttctatt tttacggaaa ggacgtgaag acatgaaaaa
    11281 aataagaagt tacaccagta tttggtcagt agaaaaagtg atttatgcga ttaatgattt
    11341 acaactccct ttcccaatta cctttacgca aatgacatgg tttatcgttt ctttgtttgt
    11401 cgtcatactc tttggcagtt taccaccact ttctctgatt gacggagcac tactcaaata
    11461 cttagggatt cccattgttc tcactgggtt catgtgccaa aagacctttg acggaaagaa
    11521 accctatgga tttctaaagt ctatcattac ctatgctcta cgccctaaag taacctacgc
    11581 tggcaaacaa atacgattaa aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag
    11641 tgaacaacta gatggcatat ccgattaaat atatggaaga taatctcgta tttaatcatg
    11701 atggcgaagt attcgcttat tatgaattaa ttccctataa ttacagtttt ttaagttccg
    11761 atgaaaaaat tcaagttcat gataatttct gtcaattgat tgcccaaaat cgtgatggca
    11821 agattcatgc tctgcaacta gcaaccgaat caagtattcg agccacacag gaacaatcga
    11881 aaaaagaaat cactggacga ttaaaagaga tagcttacga aaaaatagat gaacagaccg
    11941 aagcgttaat ttctatgatt ggtgacaatc aagtagacta tcgctttttt attggtttta
    12001 aactctacct caatgaacag gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag
    12061 catttgcgga attctttcat ggtgtgaatc acaaattaat gggcgatttt gtttctatgc
    12121 ctatggcgga aatcgaacgc ttttctaaga tggaacagtt gttagaaagt aaattatcca
    12181 gacgctttca gattcgtaaa ttggagaaag atgattttgg ttatattatt gaacatcttt
    12241 acgggcaaac aggtgtccct tttgatgact acgactacca tttaccgaaa cgatacgaag
    12301 ataaaaaagt gttggtaaag aaatacgata ttctcaaacc cactcgctgt ttaatcgaag
    12361 aaaatcaacg ctatttacgg attgaacagg aagataccac tacttatgtg gcgtatttta
    12421 caattaatag tattgttggt gaattggatt tccctaatag tgaaattttt tattaccagc
    12481 aacagcagtt tactttcccc gttgatacca gtatgaatgt tgaaattgtc gccaataaaa
    12541 aagccttatc taccgttcgc aataaaaaga aagaactgaa agatttagat agccacgcat
    12601 gggaaagcga caacgaaacc agtactaatg tggtggacgc tttagaatct gtcgatgaat
    12661 tagaaagtaa tttagaccaa accaaagaat ctatgtacaa actaagctat gtggtacggg
    12721 tatcagcaaa gaatttggac gagctgaaac aacgttgtga tgaagtaaaa gatttttatg
    12781 atgatctgtc tatcaaatta gtacgacctt ttggcgatat gctaggcttg cataatgaat
    12841 ttattccctc aagcaagcga tatatcaatg actatattca atatgtcacg tcggattttt
    12901 tggctggtct tggtttcggt gctacacaaa tgattggtga aaattctggt atttacgtcg
    12961 gttacaatct cgatacaggc agaaatattt atctccaacc tagccttgca agtcaaggaa
    13021 ttaaaggctc tgtaacaaat gcattagcgt ccgcttttat tggttcatta ggtggcggaa
    13081 aatcattctc taacaatatg ctggtctatt attcagtact ctttggcgga caggcaacca
    13141 ttgttgaccc aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat attgcccatg
    13201 aaatcaatat tgttaatctc acgagtgaga acgacaataa agggttactt gacccttatg
    13261 tgattttaaa actcccgaaa gattcagaaa gccttgccat tgatattctc acgttcttaa
    13321 caggtatttc cagccgagat agcgagaagt ttcctgtcct gcgtaaagct attcggagtg
    13381 taactcaaag caaacaacga gggttgttat tggtgattga tgaattgcga aaagaagata
    13441 cgcccatcag caatagtatt gctgaccata tcgaaagctt tgtcgattat gattttgccc
    13501 atctgttatt cagtgatggc agtgtcaccc aatccattag cttggaaaaa caactcaata
    13561 ttattcaagt ggcagatttg gtcttacccg attccaatag ctcttttgaa gaatacacta
    13621 ccatggaatt attaagtgtg gcaatgctga ttgtcattag tacctttgct ttagacttta
    13681 tccactctga ccgttccatt tttaaaattg ttgaccttga tgaggcttgg agttttttac
    13741 aagtggcaca aggaaaagct ctatctatgc gattggttcg agcaggacgt gccatgaatg
    13801 caggggtcta tttcgttact caaaacgcgg acgatttact agatgaaaag atgaagaata
    13861 atattggctt gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa aagaccttag
    13921 aattttttgg tgtggacaag gaagatgaaa acaatcagaa acgcttacgt gaattagaaa
    13981 atggacaatg tttagtttca gacatctatg gtcgtgtagg ggttatgcag tttcacccga
    14041 tttttgaaga gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat gaggtggaag
    14101 aatgacacaa aaacaaaaga aatttcttca ctatagttgg ataacgctcc tttgtgtggg
    14161 cgttatttta cttttattag gcacgctggg caatgcagta caagcgacag gcttagttga
    14221 tgaaaccatt gatacaagca atgaatactc caagtatggt ttgaatcaat atcagcttga
    14281 ttattatgtg gacaatagct ggggctggct tccatggaat tggagtgatg gcataggaca
    14341 atcggtgatg tatggattgt atgccattac taattttatt tggacaattt ctctttatct
    14401 atcgaacgcc acaggttatt tggtgaaaga agcctatgcc ttagatttca ttagtcaaac
    14461 atcccaagca attggcgaaa acatgcagac gattgcaggc attacaccga atggcttttc
    14521 cagttcaggg ttttatgtcg gtttcctcct tctttttatt ctcattgttg gaatctatgt
    14581 tgcctataca ggattggtta agcgagaaac gacgaaagtg atacgagctg ttttaaactt
    14641 tgtcgtggtg tttattctat cggcttcctt tattgcgtat gcacccgatt atattgcgaa
    14701 aatcaatgat ttttccaaag atgtcagtac cgccagtctg aatattggga caaaaattgt
    14761 tcttcctaac acggatagtc aagggaaaga cagtgtggac atgatacgtg acagcttatt
    14821 ctctgttcaa gtaaagcaac catggctatt gcttcaatat ggctccactg atgtagaagc
    14881 gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa acaatggtga
    14941 agatagagaa aatgtagtga ttgaagaaat tgaagatcat gacaatacct atttaaccct
    15001 accgaagacg atttccagat taggaacagt cttctttttg tttgtattca atattggtat
    15061 ctcgattttt gttttccttc taacaggaat catgattttt tcgcaagtgc tatttattat
    15121 tttcgctatg tttttgccaa tcagtttcct attaagtatg ttgccgagtt ttgatggcat
    15181 gggcaggcat gccattacca agttatttaa tgtgattatg acaagggctg gtattacctt
    15241 gattattacc gttgccttta gtatttccac tatgctttat tcgctgtcag caagttctcc
    15301 ctttttcatg attatgtttt tacaaattgt gacctttgca gggatttatt ttaaactggg
    15361 cgatttaatg agcctattca ctttacaaag caacgactct caaaacatgg gcagacaagt
    15421 gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac gcagtatgag
    15481 taagattttt cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa caaagcagaa
    15541 tcaaaccaat aaagctaacc atactcgtca aaatgaacgg actacaccaa agaaagaaac
    15601 agcactttcc aatgtgaaaa agcaacaagg cggtatcggg caacgattag gtgaaaagac
    15661 agcccatgta ttggacacga aaaatcgtct agtcaataaa agaaagcagg caaaacaaca
    15721 gatacaggcg acaccaaccc atgtcaaata tgcccttcat caaggaaaag agaatgtcac
    15781 tcataatgta acagacttta aacaatctat ggcagatacc aaagctcaac gcaaacaaca
    15841 gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt tggagctgga
    15901 taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga ataaccagca
    15961 gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca ctaaacgtga
    16021 aaatcatcag cgaccattga aaaaagagca aaaacaaaag acaccaccga agcaattcaa
    16081 tcagaggaag ctaaataaaa gaacggataa gcgaggacaa caaacatgaa gctaaaaata
    16141 acagtgattt gtagtgtggt cttctctctc ttcttttttc tcttattatt cctagtcatt
    16201 ttctttgcag atgatacgga cagtggcgaa aacaacaagg attcttctat tccacaaggc
    16261 ggtgtgaccg tttcaccaga agtgttggca caccgtccgt tgattgaaaa atatggtaag
    16321 gaatatggga ttgaagacta tgtttcttat atcttagcca ttatgcaagt ggaatcgggc
    16381 ggtacagcag aagatgttat gcaaagttcc gaatctttgg gtttaccgcc caacagtttg
    16441 agtaccgaag agtccatcaa acaaggggtt aaatatttca gtgaattatt aaccagtgca
    16501 gaacaacaag gagtggatat agattctgtt atccaatctt ataattatgg cggtggcttt
    16561 ttaaattatg tgaggagtca tgggaaaaaa tatacctatg aattagccga acagttttct
    16621 aaagagaagt cgggtggtca aaaagcagac tatcccaatc ctatagccat acctgtgaat
    16681 ggtggttggc gatataacta tggaaatcag ttttatgttc agttggtttc acaataccta
    16741 accgacactt ccccaacaga atttgatggt gaaaccgtgc aagtcattat ggacgaagcc
    16801 ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac ctcttttgat
    16861 tgtagtggct taatacaatg ggtgtatgac aaagctggca tttctctccc tcgtgtcgcc
    16921 caagatcagt atgacgctac gcaagaaatc tctatggaag aagcccaagc aggcgacctt
    16981 atcttctttc attcaaccta taatgcaggc acatatgtga ctcatgtggc gatatattta
    17041 gagggaaatc gcttttatca tgcaggcgac ccaatcggtt atggcgactt aagcagtcgt
    17101 tattggcaag accatctgat tggggctaga cgtgttattc ataactaaga aaggaatgaa
    17161 tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga aatctaaaaa
    17221 accgagcaag caaagaaaag ttccgatgat taaagttggc acgcataaga aattaacctt
    17281 tgttttatgg gttttattga ttggtagtgt cggatttgga atctataaaa attttactgc
    17341 cattgacact catactgtcc gtgaaacaga aatcattaaa cagcagattg tcgataccaa
    17401 ccaagtagaa agttttgtga aatcctttgc gaaagactac ttttcgtggc aacaatccca
    17461 agaagccatt gataaacgaa atgaaaaatt gacccattat ttgacggaag aattgcaggt
    17521 attaaatgaa gaaatgattc gtaaggatat tcctactagt tctagtgtga acgatataca
    17581 ggtgtggcag gtttctcaag tgaatgagaa cacctttgag gtactgtttt ctgttgaaca
    17641 agttattacc gaagacaaag acaaggaaac tatatcgtct agctttcatg tggttgtcca
    17701 tatagatgaa tcagataata tggttattat caaaaatccg acaatgagta agaaacctca
    17761 aaaatcggac taccaaccca aacagctaga aagtgaccat acagtagata cagaaacgat
    17821 ggacgaaatc actagctttt tagaaacgtt cttccagctc tatcctaccg ccacagaaaa
    17881 agaattgact tactatgtaa gtaatcatgt gttacctatg attaacaagg aatatgtttt
    17941 cgaggaattg gtgaatccta tctttaccag aaaagataac caagtaatcg tgaatgtcgc
    18001 tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg agttgatatt
    18061 agaaaaacaa gataattgga agattgtaaa ataacaaata ttggtacatg attacagata
    18121 ctttgtaatc atgtactctt tttgataaaa aattggagat tcctttacaa atatgctctt
    18181 acgtgctatt atttaagtga ctatttaaaa ggagttaata aatatgcggc aaggtattct
    18241 taaataaact gtcaatttga tagcgggaac aaataattag atgtcctttt ttaggagggc
    18301 ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag tatccagaga
    18361 atatctgtat gctttgtata cctatggtta tgcataaaaa tcccagtgat aaaagtattt
    18421 atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac atgaaaatta
    18481 ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca gaaagcttat
    18541 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
    18601 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
    18661 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
    18721 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
    18781 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
    18841 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
    18901 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
    18961 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
    19021 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
    19081 aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt gcaaaaagta
    19141 atatagggat tgataacctt atagaagtta ttactaataa attttattca tcaacacatc
    19201 gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa aaaagacaac
    19261 gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
    19321 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
    19381 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
    19441 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
    19501 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
    19561 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
    19621 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
    19681 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
    19741 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
    19801 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
    19861 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
    19921 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
    19981 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
    20041 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
    20101 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
    20161 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
    20221 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
    20281 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
    20341 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
    20401 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
    20461 aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt
    20521 tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta
    20581 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
    20641 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
    20701 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
    20761 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
    20821 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
    20881 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
    20941 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
    21001 ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa
    21061 atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta
    21121 tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac
    21181 tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta
    21241 cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta
    21301 atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc
    21361 ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag
    21421 attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt
    21481 tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca aatccccctt
    21541 tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca
    21601 gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg
    21661 actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca
    21721 gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc aactcttttt
    21781 ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga ttaagaatca
    21841 atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca
    21901 ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag ttactaaatt
    21961 tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt aaagaattca
    22021 tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc tcattgtaaa
    22081 ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc gagggtatac
    22141 ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata tggttattga
    22201 tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat cgttcgaaat
    22261 taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg
    22321 aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag gctaacaagc
    22381 caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag aaaagagctg
    22441 atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt aattcgaaag
    22501 taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac tcccgtatag
    22561 ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga gaaccatgca
    22621 gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat tcttctcttg
    22681 tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga gttttcctag
    22741 cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt agttgagcta
    22801 ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt gatgattaca
    22861 aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga agctgttgaa
    22921 gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt gcacagtaag
    22981 gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc tattattttc
    23041 gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt agtggattca
    23101 ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa ccagaagaat
    23161 taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt ttccttccat
    23221 ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact attaagaagc
    23281 atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat gtgcaaaatt
    23341 ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt caaggactat
    23401 tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat ccatctaaga
    23461 ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa gaagaatttg
    23521 aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta tttatctctc
    23581 tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt caatgggaag
    23641 atattgattt tgactcagga gtactaacca tcaacaaaac actctattac aagaatcaat
    23701 cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata
    23761 aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta attcagacag
    23821 actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca tatgctattg
    23881 aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta cggcattctc
    23941 atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat cgtctaggac
    24001 acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac agtaatttcg
    24061 aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa aattatgata
    24121 cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg aaaaaagtgc
    24181 aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag aggatttcta
    24241 gctctcctac aactattccc actccactgt tgctggtggt ttagaagtga tgtcatacac
    24301 gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt gcaagacttc
    24361 ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag cacggatacc
    24421 gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga tccctggaag
    24481 aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt cttcacgcaa
    24541 gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc caagaacacg
    24601 aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag gcatgcctaa
    24661 ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt cgatcaattc
    24721 aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg tctgtgctgt
    24781 ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga aagagacgcc
    24841 ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat
    24901 gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga agcgatctct
    24961 tgcatcgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca ttacttgttc
    25021 cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat
    25081 cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa gaccaagcaa
    25141 gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga taaagttatc
    25201 cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca gcaattcgat
    25261 tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat tacgtgttgt
    25321 gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa agccttcagg
    25381 aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg gtgtttcttt
    25441 aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt cgcggttttt
    25501 agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat agcaaatccc
    25561 taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat tctcatcata
    25621 tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt ttacttcttc
    25681 tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa tacgtcttgt
    25741 gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa cgttcgtcat
    25801 atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct caaaatagtc
    25861 aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata tgattttcag
    25921 ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt
    25981 tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca cgatctccga
    26041 tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt aacgcaccaa
    26101 aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag acagaaaaat
    26161 caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct
    26221 caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg atatcataca
    26281 catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca cggttcaaga
    26341 aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc atgattccac
    26401 gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc
    26461 ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta ctttttccaa
    26521 cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat ttatgaagaa
    26581 ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa atcagatgag
    26641 caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc aaacttttga
    26701 tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat tctcgccatt
    26761 cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc tgcgttctcc
    26821 catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa gagagattgc
    26881 cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt gacttgcgca
    26941 cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg aagacatgac
    27001 aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat gggcttttga
    27061 gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac gtgaaacagt
    27121 ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac ctgatgaagg
    27181 aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg catttgcgac
    27241 acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa
    27301 cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct atgaagcagt
    27361 tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt
    27421 tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta cactgacaat
    27481 tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg
    27541 taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg tgaaagaata
    27601 actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg
    27661 tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt tcttcaaaaa
    27721 gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata cagaacttgt
    27781 atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat actttccttt
    27841 tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga tagaagggag
    27901 ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta tcagtaacaa
    27961 ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca agaaaaaaag
    28021 tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca aatgtgatca
    28081 atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc ttcttctcca
    28141 aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc atgttatgca
    28201 attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc caccgctcag
    28261 tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca ttagacagcg
    28321 gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt cgggataacg
    28381 gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg tttattgaaa
    28441 atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa ttagaagaac
    28501 gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat gggaaatttg
    28561 ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac
    28621 ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga tgctatcagg
    28681 cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga ttcgatggat
    28741 taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca agttttgata
    28801 ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa agaaaagttc
    28861 cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg
    28921 gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag tgcttttttt
    28981 atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat gtataatacc
    29041 gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa aaaagaggga
    29101 tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata cgaatggaaa
    29161 gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag acatgacaaa
    29221 tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca tcgatttgag
    29281 ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg acacgaccac
    29341 attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa tgtctggacg
    29401 cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg gatttcagat
    29461 cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg
    29521 tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg acgtcacagc
    29581 gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg
    29641 agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga agaacatcga
    29701 agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta accgagaaac
    29761 agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa atagtctaga
    29821 agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag tagaaatgtg
    29881 ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa ccgatgaagc
    29941 aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat
    30001 gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa ttttgactgc
    30061 acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa
    30121 tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag aagaagatat
    30181 tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga caaaaggtga
    30241 gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg aattattata
    30301 taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg acatcatcac
    30361 agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag aaatggaact
    30421 aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag ctgtgatgca
    30481 aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc cagcatgggt
    30541 tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag taatcgggtt
    30601 tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg ccatcaataa
    30661 cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca
    30721 acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa
    30781 agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt gcgaaatagc
    30841 aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa
    30901 ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc
    30961 cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg ctacaagaat
    31021 cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct attaattttt
    31081 tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc tattgaagcg
    31141 ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct cgtaacggaa
    31201 tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt aacgaattgt
    31261 gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt tacgcatcta
    31321 gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg
    31381 gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg tgtgacgaag
    31441 gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct ataactcatg
    31501 ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc tttacagaaa
    31561 tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg ttatttttag
    31621 gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca tcagacacaa
    31681 caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc aaccagtcag
    31741 cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga aatgatggct
    31801 tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac caagcattga
    31861 atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg atccaagaac
    31921 aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc gatggattag
    31981 ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag
    32041 cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa
    32101 tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa
    32161 aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat aaaggacgtt
    32221 caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct tcaggcggta
    32281 caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac aaaaaagttt
    32341 gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta aacggcgaaa
    32401 agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta gaagatttgg
    32461 cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat ggattgaaag
    32521 aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa gcagttgatg
    32581 aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct gaagaaaact
    32641 aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt tgattagaac
    32701 aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa caactccctg
    32761 aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt caatcgttgc
    32821 cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa
    32881 atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat caatttacaa
    32941 gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa atctacattg
    33001 atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat gcgtgggaaa
    33061 gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat ggtccatcag
    33121 cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg aagcgagcct
    33181 agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa
    33241 cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt tggaatggaa
    33301 caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat ttttgatgag
    33361 ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta
    33421 gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat caaagcagta
    33481 gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac
    33541 gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt caaaacgatg
    33601 aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa
    33661 aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt
    33721 ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt gactggtttg
    33781 cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa taaaaaaccc
    33841 cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa atacgggtta
    33901 gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca aaaaccttac
    33961 agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga attgatcgaa
    34021 gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc aggcggaaat
    34081 cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca
    34141 aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa
    34201 caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga aattttaaat
    34261 gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa
    34321 gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga agaagcacga
    34381 aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt
    34441 attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat tgatggctgc
    34501 ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc ttggttcgca
    34561 aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag cattaggttt
    34621 ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag cactttgcgg
    34681 gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag ttgttttgct
    34741 tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg ttccaggttt
    34801 acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga actatatctt
    34861 attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc gctcaagttt
    34921 ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc
    34981 ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc ttattttcgt
    35041 ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg gattgaatcc
    35101 ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt cgatggtcat
    35161 ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt
    35221 ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt ccttgcttgg
    35281 ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt tgaagatcgg
    35341 tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc
    35401 tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt
    35461 ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa
    35521 ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat ccttacttct
    35581 acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt ttctgaacga
    35641 agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt tagttccatc
    35701 gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat cgctatttta
    35761 gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac agtcagtttg
    35821 cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc tttaggtgtt
    35881 ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca gaatctgggg
    35941 tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt ctttaaaaat
    36001 acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt gatcatcttc
    36061 aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc tgccgacaca
    36121 ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg cttgttaggc
    36181 ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc tggtacgatc
    36241 gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa tcctttaggg
    36301 gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat tggcgcacaa
    36361 ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata tttgctgaca
    36421 atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa cgggaagaac
    36481 tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg aagtgttttt
    36541 cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga ataatcatgt
    36601 ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca ccagatgccg
    36661 ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa gcaggattga
    36721 acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa ggtgtggaat
    36781 cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta gggaaagata
    36841 cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt cgtgtgtttc
    36901 ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg
    36961 tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa caccaaatga
    37021 aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc gcggcacatg
    37081 aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt gatataacaa
    37141 aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag
    37201 acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga cacacagtcc
    37261 ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc aacgatattt
    37321 tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat ggcgttgacc
    37381 agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat ttagtagatt
    37441 ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat
    37501 ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg ctaatgatta
    37561 cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga gaatatgtac
    37621 cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa ggatattatt
    37681 cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa
    37741 gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga ccaagttaag
    37801 cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag aaattgattt
    37861 tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag atgctgtaat
    37921 gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa
    37981 gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga gattccacta
    38041 ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg ccggactagg
    38101 ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg
    38161 agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt tgatcggtcc
    38221 gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata cgcctgacta
    38281 tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag aaggcgtata
    38341 tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt ctcggacaat
    38401 aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg
    38461 attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc aagcgaatct
    38521 gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta aagcattggt
    38581 taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg catattgaag
    38641 cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg cgagctaaat
    38701 atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc ggtatgttag
    38761 gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga atgggaatgc
    38821 catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag aaattgattc
    38881 gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta gtactagcac
    38941 aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt cattttcctc
    39001 caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa aagggctata
    39061 cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat ttgacagaga
    39121 cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg gctctttatt
    39181 acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc gaccatttga
    39241 tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa atgatcgaag
    39301 taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc ctgcattaag
    39361 taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg gacttattat
    39421 tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaa
//
LOCUS       DACEKQ010000014        38669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEKQ010000014 DACEKQ010000000
VERSION     DACEKQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.014"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(369..995)
                     /locus_tag="IW671_000667"
     CDS             complement(369..995)
                     /locus_tag="IW671_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897678.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="IW671_000668"
     CDS             complement(1290..1625)
                     /locus_tag="IW671_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0897679.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="IW671_000669"
     CDS             complement(1612..1896)
                     /locus_tag="IW671_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0897680.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="IW671_000670"
     CDS             complement(1952..3475)
                     /locus_tag="IW671_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0897681.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="IW671_000671"
     CDS             complement(3468..4643)
                     /locus_tag="IW671_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0897682.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="IW671_000672"
     CDS             complement(4647..4832)
                     /locus_tag="IW671_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897683.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="IW671_000673"
     CDS             complement(4798..6492)
                     /locus_tag="IW671_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0897684.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="IW671_000674"
     CDS             complement(6489..6962)
                     /locus_tag="IW671_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0897685.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="IW671_000675"
     CDS             complement(7031..7186)
                     /locus_tag="IW671_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ0897686.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="IW671_000676"
     CDS             complement(7320..7700)
                     /locus_tag="IW671_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0897687.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="IW671_000677"
     CDS             complement(7704..7919)
                     /locus_tag="IW671_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897688.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="IW671_000678"
     CDS             complement(7922..8329)
                     /locus_tag="IW671_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ0897689.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="IW671_000679"
     CDS             complement(8595..10070)
                     /locus_tag="IW671_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ0897690.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="IW671_000680"
     CDS             complement(10060..10908)
                     /locus_tag="IW671_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0897691.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="IW671_000681"
     CDS             complement(10945..11307)
                     /locus_tag="IW671_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897692.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="IW671_000682"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="IW671_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="IW671_000683"
     CDS             complement(12228..12539)
                     /locus_tag="IW671_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897693.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="IW671_000684"
     CDS             complement(12583..12876)
                     /locus_tag="IW671_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897694.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="IW671_000685"
     CDS             13070..13717
                     /locus_tag="IW671_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897695.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="IW671_000686"
     CDS             13778..14923
                     /locus_tag="IW671_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897696.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="IW671_000687"
     CDS             complement(15004..15309)
                     /locus_tag="IW671_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0897697.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="IW671_000688"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="IW671_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0897698.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="IW671_000689"
     CDS             complement(15762..16028)
                     /locus_tag="IW671_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0897699.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="IW671_000690"
     CDS             complement(16283..17008)
                     /locus_tag="IW671_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0897700.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="IW671_000691"
     tRNA            complement(17369..17439)
                     /locus_tag="IW671_000691"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="IW671_000692"
     CDS             17556..18308
                     /locus_tag="IW671_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0897701.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="IW671_000693"
     CDS             18324..18803
                     /locus_tag="IW671_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0897702.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="IW671_000694"
     CDS             complement(19054..20634)
                     /locus_tag="IW671_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0897703.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="IW671_000695"
     CDS             complement(20627..21370)
                     /locus_tag="IW671_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897704.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..22322)
                     /locus_tag="IW671_000696"
     CDS             complement(21546..22322)
                     /locus_tag="IW671_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897705.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22570..23406)
                     /locus_tag="IW671_000697"
     CDS             complement(22570..23406)
                     /locus_tag="IW671_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0897706.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23417..24346)
                     /locus_tag="IW671_000698"
     CDS             complement(23417..24346)
                     /locus_tag="IW671_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0897707.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24538..24891
                     /locus_tag="IW671_000699"
     CDS             24538..24891
                     /locus_tag="IW671_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0897708.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24965..25621)
                     /locus_tag="IW671_000700"
     CDS             complement(24965..25621)
                     /locus_tag="IW671_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0897709.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25847..27781
                     /locus_tag="IW671_000701"
     CDS             25847..27781
                     /locus_tag="IW671_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0897710.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27867..29090)
                     /locus_tag="IW671_000702"
     CDS             complement(27867..29090)
                     /locus_tag="IW671_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0897711.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29084..30982)
                     /locus_tag="IW671_000703"
     CDS             complement(29084..30982)
                     /locus_tag="IW671_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0897712.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30976..32094)
                     /locus_tag="IW671_000704"
     CDS             complement(30976..32094)
                     /locus_tag="IW671_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0897713.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32177..33010)
                     /locus_tag="IW671_000705"
     CDS             complement(32177..33010)
                     /locus_tag="IW671_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897714.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33003..33920)
                     /locus_tag="IW671_000706"
     CDS             complement(33003..33920)
                     /locus_tag="IW671_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897715.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34147..35271)
                     /locus_tag="IW671_000707"
     CDS             complement(34147..35271)
                     /locus_tag="IW671_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0897716.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35454..36362
                     /locus_tag="IW671_000708"
     CDS             35454..36362
                     /locus_tag="IW671_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897717.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36503..38116
                     /locus_tag="IW671_000709"
     CDS             36503..38116
                     /locus_tag="IW671_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0897718.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11703 a         7709 c         6344 g        12913 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata
    22021 acagttattg ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt
    22081 tctctaagca tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg
    22141 aaaaaaatat ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg
    22201 ttcaatccct gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa
    22261 atttctacta gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc
    22321 ataaaaagta cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca
    22381 tacatgttag gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga
    22441 tatcaaatac tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg
    22501 caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat
    22561 tttgttcttt taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc
    22621 gtttgtaaca actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat
    22681 ggttccttct attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt
    22741 caacaaacca aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa
    22801 gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt
    22861 gccaatgaat tcgctagggt aattataaat tagctccata acttctaaat agttttcatc
    22921 tgtgatcttg atagtttgct tatccccata cttttctttc aactcattca tttggtctaa
    22981 gtagtctgat ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc
    23041 cggatcccct actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt
    23101 agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac
    23161 ataagcgatt ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc
    23221 cttttcttct gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc
    23281 agataaaatc attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag
    23341 tttgccagaa acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg
    23401 cagcatattt tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc
    23461 cgatcagcga taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga
    23521 tatcgaccat tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta
    23581 acaaagaaga tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata
    23641 acagcatgat gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg
    23701 gtaaataagt ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat
    23761 caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt
    23821 ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta
    23881 ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac
    23941 caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat
    24001 tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca
    24061 taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta
    24121 cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga
    24181 agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg
    24241 cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga
    24301 aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt
    24361 ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc
    24421 tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta
    24481 ttcccattaa attaaggtct catacatatc acttctatca taaaagggag aataaaaatg
    24541 aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc
    24601 atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa
    24661 cttctcacac attatgatat tccggcactt catttggatg catttgaaac accagaagta
    24721 gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa
    24781 gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg
    24841 acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc
    24901 aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt
    24961 atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca
    25021 aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca
    25081 atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta
    25141 cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga
    25201 tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga
    25261 tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt
    25321 attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat
    25381 cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac
    25441 gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa
    25501 cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta
    25561 aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca
    25621 cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt
    25681 ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat
    25741 ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa
    25801 tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca
    25861 agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga
    25921 aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt
    25981 attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac
    26041 agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa
    26101 cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga
    26161 gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata
    26221 cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg
    26281 ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt
    26341 atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat
    26401 tttgattcta gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa
    26461 ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga
    26521 taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa
    26581 ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga
    26641 aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc
    26701 ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga
    26761 tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg
    26821 aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga
    26881 accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat
    26941 cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga
    27001 aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac
    27061 aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat
    27121 ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct
    27181 gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa
    27241 ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga
    27301 aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat
    27361 caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg
    27421 agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc
    27481 acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa
    27541 agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat
    27601 ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct
    27661 ggaagatcac gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca
    27721 agaaacaaaa ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta
    27781 acgaaaaaac caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc
    27841 aaacaaaaat tccatattta cctttttcac caataagaag accaattact agtcattctc
    27901 actaaagctt ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt
    27961 ttgggcttat tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat
    28021 ctttctttca attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt
    28081 ttgtctaata gacctgattc tattaattct aataatccac aaaccatgat caccatacta
    28141 gatgtatctt tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta
    28201 tcttctggca accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt
    28261 tgttctttca aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta
    28321 gcccaacagg aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct
    28381 ttgacatcca tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct
    28441 gcattttgaa tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc
    28501 gcccaataca ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa
    28561 ggatcactag gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt
    28621 aatgtttctg cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa
    28681 tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga
    28741 cagtcaatcc tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga
    28801 tcacccgtct tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca
    28861 atattgtctc ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga
    28921 ctgttactga tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta
    28981 ttccattctt tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt
    29041 atctcttcaa acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc
    29101 tttttttatt gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg
    29161 gacgattgtt ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc
    29221 cgagccaatg ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc
    29281 atgtcatcaa gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat
    29341 cgtcacttaa acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt
    29401 cttcttctat gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat
    29461 atagaacata ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag
    29521 gaataagcca agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct
    29581 ctttcttaca tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga
    29641 tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg
    29701 aatccattgc catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac
    29761 tagaataagc aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt
    29821 gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg
    29881 aaagaagttt agttccttca cgaataggca taggcatttc tttagccgtc caatactcat
    29941 ggtcttcagg tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac
    30001 tattataggg ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt
    30061 tttcatcaaa tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga
    30121 tttcacctaa tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa
    30181 ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa
    30241 aatgaatata gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct
    30301 tttctggttc tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat
    30361 aatcttggcg acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta
    30421 tattatcaaa tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa
    30481 ttgttccgac aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa
    30541 tttgcgagaa ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata
    30601 attgttcatg atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc
    30661 tttcatgtat ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg
    30721 atagaattaa tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata
    30781 aaacccgaga gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta
    30841 tataatcaat ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt
    30901 taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat
    30961 tttctcctct ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc
    31021 aataaaaaaa taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc
    31081 ttctgctccg cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc
    31141 tgtcttttgg agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac
    31201 atatcctgct aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt
    31261 cgaatctttt acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat
    31321 aaaatttact gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata
    31381 acttaacgcg aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt
    31441 gtatccttgc ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat
    31501 atgcccggtt gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg
    31561 agcaatttta gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa
    31621 attcatcata ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata
    31681 gttccctgaa atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg
    31741 actatgtttg ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc
    31801 tacatcatga tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg
    31861 tattgctgcc tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc
    31921 actccaaaaa ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt
    31981 tccgttgtaa gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt
    32041 ttcagaagct acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat
    32101 cccgccattc ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta
    32161 acattcgtta ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact
    32221 gcgcccacac ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg
    32281 cgccataatt aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa
    32341 ccgtatattt atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc
    32401 ccataaagat aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt
    32461 tcgtaaaaat cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag
    32521 gaaattgact gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga
    32581 tgatcacacc tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa
    32641 tcaatgtggt aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat
    32701 tccccataaa acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta
    32761 aaactgtact gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc
    32821 tcgccatgat ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc
    32881 cacttaaaat ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca
    32941 aaaatattaa ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt
    33001 gtttagtcat ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca
    33061 aacaccatcg tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt
    33121 ttatataaat tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag
    33181 aatatatttg ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt
    33241 aaaatgattg gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct
    33301 ccatctattt cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat
    33361 aaaatagtgt tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc
    33421 caaaaaggat ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg
    33481 aataacccag tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta
    33541 atggttggaa tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc
    33601 aataaaacag caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata
    33661 atcaaaacat tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg
    33721 tcaaatccaa caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata
    33781 atgatgggaa tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa
    33841 gcactttttt tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt
    33901 ggtaacgaag attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt
    33961 tacgccgtac ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc
    34021 tgtttgccaa tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc
    34081 tggtaaaaca gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg
    34141 tcctccttag tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga
    34201 tgtagccacg cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt
    34261 aagaaattgt tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc
    34321 tcctgatact gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg
    34381 ccatttgtca gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc
    34441 aacaaacatt gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac
    34501 aatatctgag tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt
    34561 cagacggata tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat
    34621 aaacaattct tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt
    34681 taatgctttt gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc
    34741 tatttcagca aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca
    34801 aggaataatc gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa
    34861 ctgatcgcca tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa
    34921 tttttccagc acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc
    34981 gtgtgttacc aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac
    35041 aatttttaag tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac
    35101 atagtttgtc agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat
    35161 tggatctgcg atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa
    35221 taatagggta atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc
    35281 ttctttcttt tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt
    35341 aacacaaata tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta
    35401 taatagtcat taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc
    35461 ttttgttaac aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag
    35521 atacccatct tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta
    35581 atttatatgc tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca
    35641 ttgataaaca gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca
    35701 ttatccagtt ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta
    35761 tagatcaaga aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt
    35821 atctaaaaca tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac
    35881 tatgccaaca gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa
    35941 ttcaattgtc ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt
    36001 caaatgagaa agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc
    36061 caatcatacg atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc
    36121 aaattttttt tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa
    36181 gtgttattag ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa
    36241 atgaattaac ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt
    36301 cttcagcttt taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat
    36361 agaaaaaaat ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa
    36421 ataaacaata attttccggc acaattattt gctgtttttt accataacat agtacgatca
    36481 acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc
    36541 ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg
    36601 cggagaaaat atttcttatg caagttgtgc tttgccttat tatttaggag gtgtgatcac
    36661 tgaccgtgac tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa acaacatcga
    36721 tgtctttact aaacacgaag taacagcaat cgatccttct actaagcgat taacagttaa
    36781 agacctatcc acaaatgaag aaataaaaac agattacgat aagttgatca tctcttctgg
    36841 tgctagacca gattatccgg atattcccgg agtttttgaa gcagaaaacg gctttgtcct
    36901 ccgtagtgtg acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca
    36961 tgtcgtcatt cttggtgcag gtgttatggg tctggaatta gctgagaatt tcaagcatcg
    37021 cggcttagac gtgactttaa tcgatcaatt gccacaagtc gctttccctt atgatccaga
    37081 aattgctaat ttagtttatg acaaattgct gaaagaagga ttagccgttc atttagaaac
    37141 aagagttact gagatccgtg ataaaggtcg agaaatagta ttatcagatg gctccgtcct
    37201 ttctgctgat atgctaattt ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc
    37261 aaccggcata caattatcag atacaggtca gatcattgtt gatgaccagt tacaaaccaa
    37321 tctgccggac atctatgcga ttggcgatat tatcgaaacg actagtgtag tgactggtca
    37381 gccgatccaa agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc tggcagatat
    37441 tttaaatggt acgcctatgc gctatcgcgg atatattggt gcaggtgtcg caaaaatctt
    37501 tgatcataca gcaagttatg cgggaatgac agaacatgaa ttaaaagcat caggcataac
    37561 aaattataaa actgttttta tcactccctt tgaccatgcc tatttctatc cagaagctac
    37621 aagattaaat ctaaagctga tttttgatgc agatagcggt cgtattttag gtggacaagc
    37681 atttggagaa aaaggtgtcg ataaacggat gggagaactt tctgtagcaa tcaccggaaa
    37741 cttgacagtc tttgatttgc ctgatttgga gttgccttac tctccaccgt attctactac
    37801 ccgtgacccg ttgaatatag ctggttatgt cgcaatcaat caaatgacga atatcgtaga
    37861 aacgatcaaa gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct ttttagatat
    37921 acgtgaacct aataaagcac cttctggcag tatctcggca acaaaaaata ttccaatgaa
    37981 tgagttacga gatcgaatca atgaaatccc aaaagataaa aaagtttaca ttactttcag
    38041 aagaggattg aatacttata cttctgcccg aatcttggca ggtttgggta tcaaagcgat
    38101 tttgattgaa gaataaaaaa acgaccgcgg tgggtagccg cggtctagga gttattttta
    38161 cttgaggagt aaaaatgaaa aagtatatag ggttgttggt tggtatgtat atagaataga
    38221 agataaatgt gaagaaattg tgcattattt cttcacattt attaggttat agtttaagaa
    38281 agtttaaaca ttgcgcaatt taattatttt agctgtttct tttcataaaa aaacatgttg
    38341 tgactctgag acaaatcata gtccatagtg atgtctctag agtaactacg cctaatatta
    38401 cgaggcatca tattaatcta tcagcactag tacctaaact agcatccctt tcgaatattt
    38461 tttcttatat aataaagttt gctttcactc ctctaatctc accaaatcga ctggcttgcg
    38521 ttgtgtgata ctaggaactg attggtttag aacgactttt ctattgtaat catatttttc
    38581 gccatcctcg ttgtcttcta atccttcaat gaatgatccc taatgtgtgt attaaataat
    38641 ttaatttttt tgttcctatt tatttaaca
//
LOCUS       DACEKQ010000015        37943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEKQ010000015 DACEKQ010000000
VERSION     DACEKQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.015"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW671_000710"
     CDS             complement(159..1469)
                     /locus_tag="IW671_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0897719.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW671_000711"
     CDS             complement(1481..2899)
                     /locus_tag="IW671_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0897720.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW671_000712"
     CDS             complement(3139..4707)
                     /locus_tag="IW671_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0897721.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW671_000713"
     CDS             complement(4711..5121)
                     /locus_tag="IW671_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0897722.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW671_000714"
     CDS             complement(5118..6041)
                     /locus_tag="IW671_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0897723.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW671_000715"
     CDS             complement(6038..6916)
                     /locus_tag="IW671_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0897724.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW671_000716"
     CDS             complement(6903..8240)
                     /locus_tag="IW671_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0897725.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW671_000717"
     CDS             complement(8243..9346)
                     /locus_tag="IW671_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0897726.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW671_000718"
     CDS             complement(9420..10928)
                     /locus_tag="IW671_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0897727.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW671_000719"
     CDS             complement(10894..11586)
                     /locus_tag="IW671_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0897728.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW671_000720"
     CDS             complement(11608..12582)
                     /locus_tag="IW671_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897729.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW671_000721"
     CDS             12882..13754
                     /locus_tag="IW671_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897730.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IW671_000722"
     CDS             complement(14212..16410)
                     /locus_tag="IW671_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0897731.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IW671_000723"
     CDS             16642..17634
                     /locus_tag="IW671_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0897732.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IW671_000724"
     CDS             17694..19115
                     /locus_tag="IW671_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0897733.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IW671_000725"
     CDS             19108..20607
                     /locus_tag="IW671_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0897734.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IW671_000726"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IW671_000726"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0897735.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IW671_000727"
     CDS             22199..22882
                     /locus_tag="IW671_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0897736.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IW671_000728"
     CDS             22884..23720
                     /locus_tag="IW671_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0897737.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IW671_000729"
     CDS             23731..25485
                     /locus_tag="IW671_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897738.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IW671_000730"
     CDS             complement(25761..26102)
                     /locus_tag="IW671_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0897739.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IW671_000731"
     CDS             complement(26170..28341)
                     /locus_tag="IW671_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0897740.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IW671_000732"
     CDS             28552..29409
                     /locus_tag="IW671_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0897741.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IW671_000733"
     CDS             complement(29480..30337)
                     /locus_tag="IW671_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0897742.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IW671_000734"
     CDS             complement(30322..33024)
                     /locus_tag="IW671_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0897743.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IW671_000735"
     CDS             complement(33037..34326)
                     /locus_tag="IW671_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0897744.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IW671_000736"
     CDS             34476..35522
                     /locus_tag="IW671_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0897745.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IW671_000737"
     CDS             35524..37677
                     /locus_tag="IW671_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0897746.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11210 a         7811 c         6757 g        12165 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggctc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctg
//
LOCUS       DACEKQ010000016        37157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEKQ010000016 DACEKQ010000000
VERSION     DACEKQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.016"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            115..762
                     /locus_tag="IW671_000738"
     CDS             115..762
                     /locus_tag="IW671_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0897747.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1155..3800
                     /locus_tag="IW671_000739"
     CDS             1155..3800
                     /locus_tag="IW671_000739"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0897748.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4110..5423
                     /locus_tag="IW671_000740"
     CDS             4110..5423
                     /locus_tag="IW671_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0897749.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5413..6066
                     /locus_tag="IW671_000741"
     CDS             5413..6066
                     /locus_tag="IW671_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0897750.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6121..6792
                     /gene="radC"
                     /locus_tag="IW671_000742"
     CDS             6121..6792
                     /gene="radC"
                     /locus_tag="IW671_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0897751.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6687..8039)
                     /locus_tag="IW671_000743"
     CDS             complement(6687..8039)
                     /locus_tag="IW671_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0897752.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8105..9394)
                     /locus_tag="IW671_000744"
     CDS             complement(8105..9394)
                     /locus_tag="IW671_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0897753.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9481..10185)
                     /locus_tag="IW671_000745"
     CDS             complement(9481..10185)
                     /locus_tag="IW671_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0897754.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10385..10651
                     /locus_tag="IW671_000746"
     CDS             10385..10651
                     /locus_tag="IW671_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0897755.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10819..11049)
                     /locus_tag="IW671_000747"
     CDS             complement(10819..11049)
                     /locus_tag="IW671_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0897756.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11121..11276
                     /locus_tag="IW671_000748"
     CDS             11121..11276
                     /locus_tag="IW671_000748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897757.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11585..11809)
                     /locus_tag="IW671_000749"
     CDS             complement(11585..11809)
                     /locus_tag="IW671_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0897758.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12076..12411
                     /locus_tag="IW671_000750"
     CDS             12076..12411
                     /locus_tag="IW671_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897759.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12644..13585
                     /locus_tag="IW671_000751"
     CDS             12644..13585
                     /locus_tag="IW671_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0897760.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13587..14477
                     /locus_tag="IW671_000752"
     CDS             13587..14477
                     /locus_tag="IW671_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0897761.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14520..15320
                     /locus_tag="IW671_000753"
     CDS             14520..15320
                     /locus_tag="IW671_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0897762.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15335..16186
                     /locus_tag="IW671_000754"
     CDS             15335..16186
                     /locus_tag="IW671_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0897763.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16183..16542
                     /locus_tag="IW671_000755"
     CDS             16183..16542
                     /locus_tag="IW671_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0897764.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16557..16718
                     /locus_tag="IW671_000756"
     CDS             16557..16718
                     /locus_tag="IW671_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0897765.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16715..17041
                     /locus_tag="IW671_000757"
     CDS             16715..17041
                     /locus_tag="IW671_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897766.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17038..17853
                     /locus_tag="IW671_000758"
     CDS             17038..17853
                     /locus_tag="IW671_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0897767.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17992..18303
                     /locus_tag="IW671_000759"
     CDS             17992..18303
                     /locus_tag="IW671_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897768.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18300..18530
                     /locus_tag="IW671_000760"
     CDS             18300..18530
                     /locus_tag="IW671_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897769.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            18537..18740
                     /locus_tag="IW671_000761"
     CDS             18537..18740
                     /locus_tag="IW671_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0897770.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            18737..19036
                     /locus_tag="IW671_000762"
     CDS             18737..19036
                     /locus_tag="IW671_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897771.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            19279..19692
                     /locus_tag="IW671_000763"
     CDS             19279..19692
                     /locus_tag="IW671_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0897772.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            19705..20091
                     /locus_tag="IW671_000764"
     CDS             19705..20091
                     /locus_tag="IW671_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897773.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            20642..20866
                     /locus_tag="IW671_000765"
     CDS             20642..20866
                     /locus_tag="IW671_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897774.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            20884..21312
                     /locus_tag="IW671_000766"
     CDS             20884..21312
                     /locus_tag="IW671_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897775.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            21290..22708
                     /gene="terL"
                     /locus_tag="IW671_000767"
     CDS             21290..22708
                     /gene="terL"
                     /locus_tag="IW671_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0897776.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            22720..24255
                     /locus_tag="IW671_000768"
     CDS             22720..24255
                     /locus_tag="IW671_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0897777.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            24335..25213
                     /locus_tag="IW671_000769"
     CDS             24335..25213
                     /locus_tag="IW671_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0897778.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            25215..25532
                     /locus_tag="IW671_000770"
     CDS             25215..25532
                     /locus_tag="IW671_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897779.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            25581..26282
                     /locus_tag="IW671_000771"
     CDS             25581..26282
                     /locus_tag="IW671_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0897780.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            26297..27187
                     /locus_tag="IW671_000772"
     CDS             26297..27187
                     /locus_tag="IW671_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0897781.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            27210..27425
                     /locus_tag="IW671_000773"
     CDS             27210..27425
                     /locus_tag="IW671_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897782.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            27437..27778
                     /locus_tag="IW671_000774"
     CDS             27437..27778
                     /locus_tag="IW671_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897783.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            27778..28149
                     /locus_tag="IW671_000775"
     CDS             27778..28149
                     /locus_tag="IW671_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897784.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            28142..28537
                     /locus_tag="IW671_000776"
     CDS             28142..28537
                     /locus_tag="IW671_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0897785.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            28539..28916
                     /locus_tag="IW671_000777"
     CDS             28539..28916
                     /locus_tag="IW671_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897786.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            28917..29525
                     /locus_tag="IW671_000778"
     CDS             28917..29525
                     /locus_tag="IW671_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0897787.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            29525..29866
                     /locus_tag="IW671_000779"
     CDS             29525..29866
                     /locus_tag="IW671_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897788.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            30108..32897
                     /locus_tag="IW671_000780"
     CDS             30108..32897
                     /locus_tag="IW671_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0897789.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNGGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVASSMESFLKVIAANIPRAVSFLSNLFDAVQKFGSALKFMMPFLVPA
                     TTAFGALMFQLKGIPTIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIVGAVVGAI
                     AVFGYFMATNEEVRNKVISVWNDVKDSVLGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            32887..33627
                     /locus_tag="IW671_000781"
     CDS             32887..33627
                     /locus_tag="IW671_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0897790.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            33624..36335
                     /locus_tag="IW671_000782"
     CDS             33624..36335
                     /locus_tag="IW671_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0897791.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            36348..>37157
                     /locus_tag="IW671_000783"
     CDS             36348..>37157
                     /locus_tag="IW671_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0897792.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKIE"
BASE COUNT        12858 a         5737 c         7698 g        10864 t
ORIGIN      
        1 ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa ccttgctccc
       61 gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt cgttatgcga
      121 gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata tttacgctac
      181 ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt cagcgaaatc
      241 acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga gctaggcaga
      301 agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat ccttaatact
      361 aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc attgaaacac
      421 aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa tgatccagca
      481 ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt cgcggagata
      541 gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata ttcacaatct
      601 gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc gcctgacaga
      661 gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga gttgcagacg
      721 cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt gagtaggctt
      781 attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg
      841 gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga agcataacgc
      901 tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg ctgaaaggtt
      961 ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag tagagataga
     1021 cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc aatttaggtg
     1081 gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt tttagtttaa
     1141 aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat ccgcaagaag
     1201 ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg agcggagata
     1261 aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga aaacttcatt
     1321 tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag agaatgcaag
     1381 gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca caagcaaaag
     1441 tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt gaaaagttca
     1501 tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa cagtgggcta
     1561 aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat ggattatctg
     1621 aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat cgtggtgaat
     1681 atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa gttattcata
     1741 aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga tcaggcgtag
     1801 tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt gctgttcatc
     1861 cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct ttagtcaata
     1921 aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca ggtgtagtaa
     1981 aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat gatcttccac
     2041 gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa tatgctggaa
     2101 tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga
     2161 ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt gtcgttgttg
     2221 aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg
     2281 ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat caaacgtttt
     2341 tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg tggggacatc
     2401 aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg gaagcaccag
     2461 cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt agttcagctt
     2521 tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat aagcgatact
     2581 tccctactag cactttagta accggttatg acatcatttt cttctgggta agcagaatga
     2641 ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg attcatggat
     2701 tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga atcgatccga
     2761 tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg aatggttctg
     2821 caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg aactttatca
     2881 acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg accgtagaag
     2941 agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact cgtttgaatg
     3001 aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa gcaggtcgac
     3061 aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg agtaaagaag
     3121 tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta gtccatacac
     3181 ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa gaaatatggg
     3241 aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt gtacgtccag
     3301 agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg attcgagccg
     3361 ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg ttgctaatta
     3421 aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat atcgaacgct
     3481 tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat ctagcgatgt
     3541 cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc aatattgaag
     3601 aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc
     3661 aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag
     3721 agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa
     3781 gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta gctattacta
     3841 aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct tagcttcgat
     3901 tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact cttattatgt
     3961 aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga taaaatcgtt
     4021 cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt tctatttgtt
     4081 ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata gaatggatcc
     4141 atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct ttattagaag
     4201 tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca aacggaaaag
     4261 gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca gtaggtacat
     4321 ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc aaaagcattc
     4381 cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa caacttgatc
     4441 aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg gcatttgatt
     4501 attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc ggcttattgg
     4561 attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga cttgatcata
     4621 ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga atcatcaaac
     4681 aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc atccaacaaa
     4741 cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa ataaactatc
     4801 tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata ggaaagatcc
     4861 ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga gtagccatcg
     4921 aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa gatgttttga
     4981 agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac gagccattag
     5041 ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag aatttgaaaa
     5101 aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact aaaaatgtta
     5161 gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg acgacctttg
     5221 aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag agagtatcaa
     5281 tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca aatgaagacg
     5341 tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta ttaatacaat
     5401 taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt gctgttcgac
     5461 acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg ttttccttat
     5521 gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg ggcgaattat
     5581 catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga tgattcatac
     5641 caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa
     5701 ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag taaccaacga
     5761 gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga gacgattgtt
     5821 tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt agctaatcag
     5881 aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa tggtatttta
     5941 gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc accatcccaa
     6001 gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc tgcattgtta
     6061 aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt
     6121 ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa
     6181 atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt
     6241 caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg
     6301 gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg
     6361 aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa
     6421 agagcggctc caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg
     6481 aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc
     6541 ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt
     6601 ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttcc
     6661 ggaatatata agttagtaca cataaattat ataaggaaat ttactgttat attgccatct
     6721 ggaaatacta ttatgttatc gataattgaa cgccataatg catttttttc ttcttccgaa
     6781 gattctttat aaatttcatc gaattctttt gatagaataa attcgacatt ttttatatct
     6841 attttagctg gctctacttc aatttcattt aattctttca ttaaagcttc tcgatctttt
     6901 ttgtattcct ctaagtctat aacttcattc aagtaggctt tcttcaatct atctaattta
     6961 ttctcaatat ttttcttact atttttcttt ttaataatct ttggttgttt ttttgaatag
     7021 tttatttgta tttgttcaaa tttttgttta gtagcttcaa aaatataacg ttctaaattt
     7081 ttttcgtaaa aagcgcgctt atttgtgcaa cgtaatttat tatttcttcg ttttacacat
     7141 ctataacaag tcctttcgta tatagattct gtaccgtctt ttttcttata tcttgcttta
     7201 atagtttgac cagctactga tccctgacag tcagaacatt ttaataaacc tttaaaaatg
     7261 taatcacgtt ttgcatttgt ttttaaattt ttaggtaagg ctttttgaac agcataaaaa
     7321 agttttttat caataatagg aggacagtaa tttttattat ttcttaattc accaatgtat
     7381 tttttgttcg tcaacatatt tttaacactt tgataatctc taacgattcc atactgagag
     7441 cctaaataat aaacagtttt tcttaaacta ccactagaaa gaaagtagtt gaatatatct
     7501 atcacgatag gagcatcatc atttattact aatttcttat tttctataga gtagcctaac
     7561 ggagttttcc cgcttactac ttcaccttgt gcaattttat ttgccataac agctttgatg
     7621 cgttctgaat caatttgggc ttccaattca gcaaaactca ttacttgtgc aatgaaagtt
     7681 ctgccatacg ctgtagttgt atcataatat tgttgagaaa cagcattcca agaaacattg
     7741 tgtttttcta aaatttcttg agtgtttaaa taatgtcgta gatttctaaa ccatctatct
     7801 aattttgtaa ataaaattag atctatttta ttttctttta catcttgtaa tagtttttga
     7861 aattcatcac gatttatttt ttgaccagaa ataccaccgt ctacatattc attaactact
     7921 ttcaaatcct tgtgttgttc gatatattca tagagggttt ctttttgttc tctaagacta
     7981 tctccatgtt tggcttgttg ttcagttgat actctcatat ataatgctac tcgtttcatg
     8041 ttatctcctt tcgaatgtat gttcttatcg ttgtaaaaga aaagcccgaa ggctaatctt
     8101 tttactacac atctgttaat tctaatgatc ttctgtattg ttcagcttga gctagtttag
     8161 taaaagatac agttccgtta taattttcta aaacaacttt ttcaatttca tcaagagata
     8221 ctttaaagaa ttcttttcgt tgattaactt tattgacttc gttttttcta aaatgcgaat
     8281 gtagttgatt ttctaatgtt ggtgcatctt ctgaaaatat cattgcatga acatcaaatt
     8341 caaaaggtac agaagcgcta ctaagttcct taattctatc cataggttct aaccgtctag
     8401 tcattcctat tttatagaca tcttcaccga aagatccgat attactgatg acataaacat
     8461 agccagctct agtattaagt tctctttgca atacatcttc tttgacagtt tctagttctc
     8521 ttaatttctc ttccagttct ttaattttat cagcatagag ttctttttca acatcagcac
     8581 tagctttttg catgtatgcc attaacttag ttatctctga gttaaactgt cgttcttctt
     8641 tatcaatgcg tttcttttca cgttctattt cacgtcttac cttttcttct tcaatcattt
     8701 gttcttttat tgctttttgc tgttcttttt catcgttaac tttaactatg tatgaatagt
     8761 tgagttctaa ttcttgaagt tttaattcaa gtaattcttt tttgaattct gcttcatcgt
     8821 ttttaaaaag cttgtttatt ttatcaaaag ttcttataat tttttctcta gttgaatcta
     8881 cgttagaaat agtaagttta ttgagaagtc cagttatttc tgattctaat ggtataagca
     8941 ttttcttttt aatattatta gcttctctat tattcaaata agaaggaata ttaattgcac
     9001 cgtctaaaat aaaatccttt tctttaatct ttaataaaga aagtttgttt tttatttcgg
     9061 atgaagaagt ttcatctata ttcggaataa ttactgattt atagatagtg accagagatg
     9121 tgtcatattt ttgattaatt tcatcgtcta accgctggct ttcagcatta agaatatcaa
     9181 ttttgttttc tagcgttgac aagtttttat tcaatttact tatgtttttc tctttttcag
     9241 atataaacgt ttccctagca tttaattctt gagaatattc ctttacctta ctctctaagg
     9301 cagaagtttt atcttttaat tttttagctt cagataattt gctactaccc ataattatta
     9361 aaactattcc aactaaaaac aatagtactc ccattcaaaa cgcctcgctt cttttataaa
     9421 ataattgtca aaatcatata aacccttacc gaagttgacg gctccggcat gggaattttt
     9481 ttaaaataca acttttccga taattctaat ctgttctggc gatacaattc tatcttcgta
     9541 taagtcgttt atagaacgta aaatgattgt ttgattttcg taatctttat atatctcttt
     9601 acatgtaact ccgtcaccac ctaattccac aatagcaatt tccccatctt ctacgtccgg
     9661 ttgttcttta acgaatacga attgtccatt cctgataaca ggttccattg aatcgcctat
     9721 aataggtact gcgaagtcag cagcacttgg aactctctca aattcttcct cttcgacaac
     9781 cgtatcaccg taagtaagtt ctgttggatt ggcagccgaa gccgaattgt gaacagttgg
     9841 gattttttct tttttaggaa aatctaggac attttcttgt ttttgttctt ctaatcgatt
     9901 acttgcaaat tcataggtag caactttatt ttcgtcgtta agctgattat atataggaag
     9961 gatattatat atctgattag atatagataa atcaatattc atcaaatcat ctatatcaac
    10021 attgaatatc ttagctatct catttaaggt ttttaatttt ggtgtatatt ttcctttttc
    10081 ccattcgctt attgaggatg aactttttct tcctagtttt tcggctagtt cttgttgatc
    10141 aatattatac ttttctctaa gaaactttag attttcagcg aacatagtaa cacctccttg
    10201 agttaattgt aacaccgttt cggaaaaaat gtaacaggtt tcttagaaaa tattttagtg
    10261 tttttaaaga agtatttacg caaaatagaa atcgatttca ggaaaactga aatatgcact
    10321 tgacttcgga aaaaccgaag tatataattt agtcagaaag ttaaagagag gaggcgaata
    10381 aaatatggat accgcgaaga aagaaaaata tacattgaga caattaagag ctttaaaggg
    10441 ttattcaaaa gaagaactgt caaggaaaag caaagtgaca tcaagaacaa tatttattta
    10501 cgaaaacgat gtagataaaa tgaggaacgg aaagtacgcc actctagaaa aaattgctaa
    10561 ggcattgggt gttagagttt ccgatatttt tttagaccct gattcggaaa aaccgaaatc
    10621 aataacaggt gaaacaaaac aagtcagcta ggaggtgtga atagtggaag agaagaatat
    10681 aaaagtaccg actcttgaag aatatacaaa aattgttgtc gaaacagacg agcagaaccc
    10741 gaaaacaatc gcagttatta cggcgaatga tattgaatca tgcgaaggtt ttcgagttcg
    10801 aatgacccct agatatgatt aatgttcttt atcacgtgga ggtataggat cgtttccaaa
    10861 actatctttt gattgtattt gtccattctt tttttggaca ataaactctg attgctgttt
    10921 tttagaaatc tttttaccat actcaacagc atctgattta ttatcaaata gttttgtatc
    10981 ttcggaatta ccttcaccac gtacagccca tttgccattt ctaggagata cccattgatt
    11041 tttacccata acaattcacc tcgctttcaa gttaattcta ttaagaggtg aaaagaaaac
    11101 aaactgatgg aggtgagatt gtgagtaaca agccaaaaat cactttgaat ggcacaccta
    11161 atcttcaaag attagcagat gaaatggcta gaatacgttc tagacattgg ggagttactg
    11221 tggtggcaac cataattgaa caaaaagaaa agcccacaca gccggcaagc aagtagtggg
    11281 caccatagaa caataatatt atatcacaca ctcgccactt ccgaaaagag gagttgctgc
    11341 tctcctttca agtttgccaa agtccacgtt tcttcatttt ggcaactcct cttcttggag
    11401 gtggtgacgg cgagagacga aggagggatg acatgaagga agaatcaaaa atagggattt
    11461 tgaatgagaa caattacaca agattaatta ttgaaacaga agaaggggaa aaagtagcag
    11521 aagttacatt gaccgatgca actcctgctg acggttatcg tattcgatta actcctaaat
    11581 atgattaacc ttttggtggg tgtggatcgt ttccgtgact atcacggctt tgaatttttc
    11641 catctctgcc atgaataaat agctcagatc cttgattttt agaaatctct ctagcgattt
    11701 ttactgcttc tgattttgta ttagtatgtg cagtcgcttt agaatttcca gcacctttga
    11761 cattccaacc accattggaa ttaggtacaa catgttgatt ttttcccata gttttcacct
    11821 ccatttctat agaaatattt tatcaaatag aaatggagaa attcaataaa cgaaaggagg
    11881 gatcaaatga cattacaaga gcatcaacac ttgatgcgaa aactaaatca ggaataccac
    11941 gaggaaatga aacctagatt gctaggggaa caatggcgtg aacgccagaa gaagtggtta
    12001 gaaataaaaa agtgactcag ccgtccaaag caatgagtca caaaccaaaa attataccta
    12061 aggagatttt atcacatgaa taagaaaatt gaaaatctta tcgaagaatt gaaacgtgaa
    12121 tgccaaaagc aaggtgtttc tattatttgt acagcccaaa aagaagggga acttaaaagt
    12181 ctcgtttatg gtgagacaac agaaatttta ctttgtcttg ctatgcaaga agaacattta
    12241 gatgaaaatc tcccattgtc agcacatatt atgcgtagaa ttgctgtgga tgcttacgaa
    12301 caagcgaaaa atgaagaaga aaatcaacct tcaaatcata cttttgttat taataacaag
    12361 gaagatttag ctgatgtgat gactcgtatt ctcaaggggg aatttcaatg atgaatgaaa
    12421 aaacaattga tctactcatt attttatttt tcacgttagt gattgtagcg aaaagttatg
    12481 taatctttgg cttgttattc gcaattacca tgcttgtgct ttgcgtgtta gcaagcagaa
    12541 aagaggttaa acatgaacga gttagcaaat cttgacaact atttaactga tcctgattat
    12601 gctaaaccac catatgaagc gccaattgat gaggaggatg aagatgagta aatctacctt
    12661 agaaatgagc catcaagaat ggctcgaaga ccgcaaaaga ggtattggtg gctcagatgt
    12721 tgcaaccgta cttggattaa acaaatacaa atctccttat caattgtggc ttgagaaaac
    12781 gggtcaaatt gaattgaaag atttagaaag cgaaccagct tattggggta atgttttaga
    12841 agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa aaagtacgca gaagaaacca
    12901 agtatttgaa catccattac atccattttt aagagcaaat attgatcggg aagtagtggg
    12961 agaaaatgcc attctggaat gtaaaacagc caatcaattt ctcggaaaag agtgggaagg
    13021 tgaagaggta ccgcttagct atctctgtca agttcagcat tacatgaacg ttttaaacaa
    13081 agactattgt tatatcgctg ttttgatcgg tggacaaaaa ttcatttgga agcggattga
    13141 acgagatcaa gagctgatcg atacaatcac tgaacaatta gtagagtttt gggaaacgaa
    13201 cgttcttgga ggtatcgagc ctgtaattga cggaagtgga gcgactgctg acttcttaaa
    13261 agaaaaatat gcagatgtag aagaaaatca aacagctcta ccatcacgtt ttgatgaact
    13321 tatcgagcaa aaaagagaac tcaagcggac gaaaaaagaa attgaatcat ctattcgtca
    13381 agtagacaac gagatcatca gtgagctagg taaacgtgaa gccagtatcg gtatcacaca
    13441 aagaaacatt atcagctgga aacttgttcg tacaagacgg atgaacacga agaaactagc
    13501 agagaaatat ccagatgtcg caaatgatga agagatttat aacgttactg aatcaagaag
    13561 gctaaccgaa aaggagatca aataatatgg caacaaatga atcgttaaaa aatcaattgg
    13621 cagaaaagcc acagaaacaa gttgcaccag gacagttagg gcttaaagct ctaatgaata
    13681 caccaacaat gagaaagaaa tttgaagaag tacttcatga caatgctaat gcttttatgt
    13741 cgaatgttat gactcttgta tctaatgaca gttatcttgc agatagtgaa ccgatgtcta
    13801 tcatgagtgg tgcgttaact gctgcaacat taaatcttgg gctagataag aatttaggtt
    13861 atgcatattt agttccattc aatagtaaaa acaagcaaac aggaaaatgg gaaaagaaag
    13921 ctcaattcat gcttggctat aaaggatata tccaattagc ccaacgatca ggtaaataca
    13981 aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa aagctggaac cgactgacag
    14041 aagagtttga gtttgatcca aatggtagaa cgtctgatga agtcattgga tatgttggct
    14101 atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa gaaatcgaag
    14161 ctcatcggat tgctaacaat aaagatcgcg ataagacaaa gttaagtggt gtgtgggcat
    14221 ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa tcttctttct aaatggggaa
    14281 tcttgtccat cgaaatgcaa gaagccacca catcggatga gagagtccaa agggttcaag
    14341 aagacggcag cattattgct gaaacagaag ttgaagaaga tattcctgaa agaaaagaag
    14401 cagaggttat atctgaagaa aatgaagatg tacaaactgg attatttgat gcatctaatc
    14461 cgccgttaaa caaataatga gggagctttc tcccttgctt ttctagagga gaaatacgaa
    14521 tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt tggaccaatc
    14581 cttatatgta taaattatgg agcttgtgtt tgatgaaagc gagtcatgag aatcgcaaga
    14641 tactttttaa tggaaaagag atacaagtga acagcggaga attcgtcaca gggcgcgatg
    14701 ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac agcggttctg
    14761 tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa tcaactacga
    14821 aatacagcgt tatatcaata aaaaactggt ctttgtacca aagcagtgaa caacaaatga
    14881 acatcaaacg cacaacaagt gaacaacaag tgcacacaaa caagaatgaa aagaattata
    14941 agaatgaaaa gaataataaa acatcatcgt cgcaaccacg caaaaagcgt gtttacgaca
    15001 ccgactcagt ttactacatt ctcgcggagg agttattcaa acagatttgt cagaatcagg
    15061 aaatcaaaaa gccgaatctg caaagttggg cagataacat tcggaaaatg atcgagatcg
    15121 acaagcggac cgaaaaccag gtacggggaa tgattgaatg gagtcagcac aatgtgtttt
    15181 gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa atacgacaca atggcagcgc
    15241 aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct tgaatacgag aaatttagca
    15301 cagatgagtt gcctatttga gaggaggcgt aggcatggaa actgttgggg aaatcatgga
    15361 aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg gacagccttt
    15421 gtatggatgg cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa cctgtatgca
    15481 atgtgggtat aaagcattgc gagttcagga agacttacaa acagaacgaa tttacaacga
    15541 gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc ctaatcaagc
    15601 gttgtttgat tgcacattgc agaattatca aattgtcgat caagaaacaa gacaagcggt
    15661 tgaagtaacc aaacgctttg ttaattcagt cttgttagga aatccaagtc accttgtttt
    15721 aactggaaaa caaggaacag gtaaaagtca cctagcgatg gcagcggctt gggaagtctt
    15781 gaagcgatca aactacgata aaaaaatctt atttatcggg ttacaggaaa tgctggatca
    15841 aatcaaattt tcgtacaaca atcctgaact cagaaaaacg attgagggat cgttgattgc
    15901 agatatcaaa acagcggatt tagtcatcat tgatgatatc ggttcagaac taggaaaaga
    15961 tgcatcagat agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg atgcacgaca
    16021 gaacctagca acgattatca caacgaactt gcttggtgaa gaactgaaaa aagcttacgg
    16081 tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg tattttctca
    16141 aacagcagac aagcgcataa aaccagtgaa aggtagtatc gcatgaataa ataccgtaat
    16201 aaaaaaactg ttcatcgagg tatcaagttt gattctatcg cggaagcaga gtattacgat
    16261 ctagccttgt ggcaagctga agcgaatggt tggaaagtga agctccagga aaggtttgag
    16321 ctgatgccga agtttgaact agagggcaag aagtatcgca agatcgagta tattcccgac
    16381 ttcacatttt acaaaagcgg caagctggtc aaagtcgtag atgttaaggg gatgcagaca
    16441 aaagacttta agatcaaggc aaagttgttc tgtcatcaat atcaagtgcc gttgatatta
    16501 gccaaaaaat atcggaatac gttcaaggaa gagcgttttt aacgaggtgg tccatcatga
    16561 caacagaaga agtgattcaa atgcgaattc gaagccttcg gcgtgagatt gacgatctgg
    16621 aacgaacaaa ggcagtgatg gtcaatgaaa cggctagaaa ggcaatcgat ttgcacatag
    16681 agaatttaag aagggaaatc cgtagattgg aggaatgagc gtggataagg aagcggcttg
    16741 gcgaaaatta atgttgctga ttcaagatga gaactggcaa gaagatgaag cagtggttgc
    16801 tgaagttcag cgtctaggaa agattgctaa cggacgtata cgaaaaaagc cagacaaaag
    16861 aaaacagcgc aaagggaaaa tcgtcgttgt tttacacgaa ggcaaaattt tgatgcaagg
    16921 aacagctagt gagctgtctg cagaaactgg atatacgcgt gggactattc gaacgtacgc
    16981 ttggcgaaat cacaccgatc gaaaagggca cgaatataag tatttggagg aagaaaaatg
    17041 aacgaaaaca aattaatcaa attgggtgta gcaggagcag taatcgtagg tattggagtt
    17101 atcggaggat ttaagttctt cgaaaaaatc gataatggat atgtgggtgt gcgctattca
    17161 atgaacggcg gtatcaaaga tgaagcactg acgcaaggtg tgaaatttgt agggattgac
    17221 aaagtgatcc aatatccaat tcgcttgcaa actatccaat caaaaaatat ttcagtatct
    17281 acaagcgacg gcaaaaagac aacgattgat atcaaatatg actacaaagt tgattcaact
    17341 aaagcagcaa aaatgtacaa ggaatttggg aatatcactt cggaagatat cgaaagtgga
    17401 tggttaaaat ctaagcttca aaaggtcgct cgtgaagttt atgcgaaata tagtctgctt
    17461 gatgtccttt caggagattc ctctaaagtt gaagctgagg tattaacgaa ctttgctaaa
    17521 tcagttgaat ctaaagggtt tgaagtcgaa gacgtaacac ttggtgttcc agatgtcgat
    17581 aaagaaacac aaaaatcaat cgatgcgatc attcgagctg gtcaagaaaa tgaaaaagcg
    17641 aagctagacg cagaaactgc aaaaactcaa gctgatagtg aagcttacaa gaaaacaaaa
    17701 gctgcagaag cagaggcaga atctaatcgc aaagtcgccg aatcagtaac agacaatttg
    17761 attcgttatg aagaagctca agctcgcaaa aagcatggat gggtaacagt aaatggagca
    17821 gatactgtag ttacggatga agcaggcaaa taatatggga ttctttatgg ctaaaattct
    17881 cttgttctta ggtttagtag gagcagcata tctcgtgtat gcgctccttt cccaaactga
    17941 tgacaaagaa gatgacaaca acgatgaaat gaaattttag gaggagaaat aatggacgaa
    18001 ctaatcacaa aagtagagca gtgggctaaa gacaagggac tggatcaagc agatccaaaa
    18061 gcacagtttt tgaaagtagc tgaggaattc ggggaaatag cttcggcgat ggcaagaagt
    18121 aatgatgaac tatttaaaga tagcgtagga gacgttatcg tcacgctgat tatcctttcc
    18181 atgcaaaaag ggacaaacgt acaagagtgt ttagaaatgg catacaacga aatcaaagga
    18241 cgcacaggga aaatggtaga tggtgtattc gtgaagtcga gtgatttgga ggatgtgaaa
    18301 tgaaatacga aataccacta agtgaagcgg gcattcaagc aattatcaat ggtcgggagg
    18361 ttaacataga acttcctgat ggtactgaat tagtcatcag acaaagttat ttgaaagata
    18421 tggcagctcc agtattaatt gatcgtttta acgtgactga ttctgtggta gagaaccact
    18481 taaaagaatt tcgatcaagt atagacgaca ctttcagatt agggagttga ttgacaatga
    18541 acaacagaca tcgcagaata acaaaactaa gaaaacagga actgaatgta ctaaagacaa
    18601 agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca agtcagtttg
    18661 ttgctgatgc aagtgatgct attcgtaagt ttgggatttc gatattaaat gatgatcgta
    18721 aatgggagga aatgagatga aactaaaaga cggattttac gctagtagtc acggcatcgg
    18781 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtaagaaac caaaagtcaa
    18841 agtcggtgac atggttcgct gtgaagcaga agagttcatt tatccgtttc gtggatatgt
    18901 agaacacgtc tataatcact cagcgatcat tcgtattgaa aacacaatgg aatgcgataa
    18961 gtggttagcg aaaagcaaag agaatttagc tgtagcgaga ttggtggata tggaggttat
    19021 aaacaatgaa gtttaaaatc tttgaagagg acactcgtta taaattagaa aaagaattaa
    19081 acgattttgc gaaaaacaat gagattcagc atatatcttt agcaacttct aagagaggtt
    19141 atgcaaatta ctatgcagct gttgtgagct atgtaagtcg agaagtgtaa ctcggcaaat
    19201 aaaaaaagcc ggatcgctcc gactgattca ataaatccaa cacatttatt atatcacata
    19261 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
    19321 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
    19381 atagatatta agtcgccgat tattacggat atgccgaagg caccgaagca cggcaataag
    19441 gcagaagacg cgatcattca aatgatggat atagaagcgg agagagacgc gattctagtg
    19501 gctttgatgg ctcttagtct gattagtcgt cagatactct actacagctt ctgtgtgcca
    19561 gacagcttct caaactacag aattagccgt gaagtgggtt attcagaaag aagtatacaa
    19621 cggatgaaat cggaagctct aatagagttt gcagaagcat ataaacacgg aagaataatt
    19681 gcttataaat agggggacca tataatgtgg aaagattatg tatcgttgaa agagttgaaa
    19741 aaagatcttg ttttcaaaag aattgtagaa tggtcagaga gtgaattaat tttagaagat
    19801 ggaaccaaaa tggaagttgt atgtagcgaa tcagattgct gcgcatgggc cgaaggtgaa
    19861 ttcaaaaatg taaagcttga tgcagtgatt acagatataa agatttttga taaaggtaac
    19921 cgtctctata atggtgacgg acatacctct tatgctgaag tcgttgttta tcataatcgt
    19981 aatgagatcg ctaaagcaga atgcacagcg aatgatggga atggcggcta ctattatagt
    20041 gtttgtgctt taaaggtcaa agacaaactt tgtatagtga ctgacgcata aaaaaatggc
    20101 ggttttttgg cggaatgatg gcggttttta gccatttatc agtgatatta tggtagtgtc
    20161 gaaagattag tgataggtct gagacaaaat aataataaaa ggaacatcgt tttattattg
    20221 tttcacaatt aagcttcgat agacagcagc ggaaatatta agaataagga tgtgaatttt
    20281 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt tacatacaga
    20341 tcgcttaggc ggtcttttta ttttggccga aaacctacat tttcgatggc cgattattgg
    20401 aggaggaata gcatgttcaa actatctgaa atcattaaga agtccgacgt tgagaaatta
    20461 gagatgttaa aaaagaagtt gaagaaatag cagtctcttc gtgaggctgt tttattttgc
    20521 tcacaaaaat agaccactat cgggtaatag tggtcaggaa ttaaatgaaa aagatgttag
    20581 ggttgttagc taagtataac atcataacgc ttacaaaaca atgcaagaag gataaattat
    20641 tatgagaact tactggtatg tgtcattaaa caataaatat ccgaagccaa tgaaaggaca
    20701 gcataggcgt gtagtcatgt ctgttcaaat gaaggcgaag tattcgattg tagaaatgat
    20761 cagagaggca acgccagtag agattgatta ttgcaagcta gtctattgtg ggtgcggccg
    20821 ttggaaagag gatcatgtac aaaaaaatat cagcaagtac atatagagag gcggtgatta
    20881 aggatggaaa aaagcgatac gaaagcgaac agatacaaac ctactgctgc agagaaaaaa
    20941 ctcttggaag tattgataaa tccagataac ttaggcaaac cggtgcaaga gttgtgttca
    21001 ctggcgaaaa taggtaggac caaatattac gacgccatga gcaaagatgg ctttgttgat
    21061 ctagttaacg aaacaacaat ggatctgatc aaaggaaaag cttctgatgt ccttaatgcc
    21121 acttacaagt atgcattgac tgaaaaaggt catcaggata ggaaaatgat tctgactatt
    21181 gctggaatct atgcagacaa acaagaactc aaacattctg gcggcgtcga tatccgaaaa
    21241 cagtatgaga aaatgtccga tgaagaatta gaagagttgg tgaaacggta tgaaaaaatc
    21301 aacgactctt aatcgtgttg agtatgccgt tgttcaaaga gagctagcca ttcgaaaagc
    21361 taggaaaagt ttttggctat tctgccagct tctttatcca gagttttaca aagatgatcg
    21421 gttgtatttg aaagatttat gtcatacgct acaagatttt tacgaagata aaatcgacaa
    21481 aaaaattttg gttattaaca tgccgcctcg acatgggaag acttttacag cgcggctatt
    21541 cgttttatgg atgtttggac aaaatccaaa gacgaaaatt attaccggat cttataacca
    21601 aatcctgtca tctttatttg cgcaacaaac acgtgatgga atcatggttg aaaacgaaaa
    21661 tgttaaggct gaatacttta aagatatttt tccagaggtg caaatcaaat atggagatgc
    21721 tgcaaagggt ttctggtctt tagatggttc agaagaaaag aattatttag cgacttctcc
    21781 tggtggaaca tccactggta ttggcgcgaa ttatgtaatt gttgatgaca tcattaaaac
    21841 cgcagaagaa gcctataacg agcgtgtttt agattctcac tgggaatggt ataacaacac
    21901 gttggcccaa cgaatggaac ggccacgtaa acagatattg attatgacac gttggtcaag
    21961 taatgattta gccggaaaaa tgttgacgag acgaaaaaat aatgttcatc agatctgtta
    22021 taaagcagta caagaagacg gctcgatgtt gtgcaacgag attatgaccc acgatgagta
    22081 cttagatgtg gtacaagaaa tgaatgtaga tattgcagaa gccaactatc agcaagaacc
    22141 aattgaccaa aaaggcaggc tatatcagaa gtttcttact tatgatacgt tgcctgataa
    22201 cattatcaaa atatggaact acaccgacac cgccgacaag ggtgccgatt actttgcgtc
    22261 tcctgttttt gctgagacgt ctgatcatga tgcttatatt atcgatgtca tgtatacaaa
    22321 agagccgatg gaaacaactg aaatagccca cgctaatatg attattcgca atcgagttaa
    22381 taaggtacgg attgaaggaa acaatggcgg gcgtggattc agaagaaatt cagaaagaat
    22441 tacaaaagag cgaggctatc gaggtgcagt atttgaagac tttcatcaat cggataataa
    22501 actgtctaga atactggcga atagtgcttg ggtggaaaat catgtttact atccagatga
    22561 ttgggcgaca cgttggccgg acttttatga ggctatggtt agttatcaac gtgaaggtaa
    22621 gaacgctcat gatgatgctc cggatgcagt tacaggaatt gcagaaacga ttatagaagc
    22681 agatgacttc gagtttattg cttattagga ggtgggtctt tgttttattc tggaatcaca
    22741 ttgaaaaaat acaaacgttt aagaacgaaa tattcaacgc aaatcgaaga aggattattc
    22801 gatcctaacg cttttatgga agatatgaaa ccctttttcg atgatcgaga aagaaagtat
    22861 cttgcttata ctagcgagaa aaacgagatc gacagtcgtg agaaaccaaa aacaaaaatc
    22921 attaaggtca ataacaagat tcatgcggga atgtattcag tggtcgttga tcaagcagtt
    22981 aaccatttca caggaatacc aattaaatgg gattatgatg tttcggaaca gaaaaggaca
    23041 ttcattcaac gaatgagaga caaattactg ggtaatgata tcagcgcccc ttctacccca
    23101 gaagaatttg aaacactcac gagacaatta agtactatgc ggtttggaat gttagacccc
    23161 gatactgcaa catttcaagg agcgacaggg cttgctttca gattactgga gcctgttgag
    23221 acaaatgatg gttgggaact aagagcaagc aacattgagc cgtggaaagc tgaaaagtac
    23281 gaaaacggcg cagtgtacat taaagagaaa tacgatccat atcaaaagaa gttttatgaa
    23341 gagatgaagg ttgtcacgcc gaaatcaata tttacttatg ctcgatatac tgattaccct
    23401 ggcacggtac aaggagcatt taaaacgctg ggtaatgttc ctaatccttt aggcgttatc
    23461 aatcttacgg agtttaaaaa caatacaaac cgttactgtg actttgaagt tgcagaggag
    23521 attagtgatg ctattgatcg tgttttatct gatcagcaga acgagattga acaattcaag
    23581 ttagcttata tgctgatcac tggggcagat ttgtccgagg ataaagccaa gaagatgatg
    23641 gagcaactag ggatcatcaa tctgaaagac cctaatgcga aagccggcta cgtgacaaag
    23701 gatctacaaa aggatttcaa cgagtatcat tttgatcttc ttaaaaagca attctacacg
    23761 gtgtgtaaag caattgattt caatgacgaa gtctttaagt cgaatagttc tggggaagcc
    23821 cgaaagtggc aaatcattag tttagaagct aagacaaata ccaaagaaca gtactttcga
    23881 gaaggattga aagagtgtgc cgaggttatg gctgcttatt tggattttgc cgagaaagtt
    23941 acaattgatc cggaaaagat cattttcacc ttctctcgtt cgcttccaac agatttaagc
    24001 tacctagctg aggcattgcc taagttggca ccatacgttt ctaagaggac gattcaaagt
    24061 cagatcccgt tcgtcacaga tgttgattat gaaaacgaaa tgattgacgt cgaaagcaga
    24121 caatcttacc cagacggcga gtatggaggt ggcatcgttg agtcaaatag aaacgaagta
    24181 ctggaccaaa cgtcgagaac tggaagattt agcaagactc aaaatggaaa atcaaacgct
    24241 aaagaaacta agtagtgtct ttcctgatgc gttgaaagaa attcaagcgg agttattatc
    24301 ccaagctgat ttacacgata ttacctatgc tgagatgatg gaattttata gtacaagtga
    24361 tcagaaaaaa tatcgtgaat acgttgaaaa gaactacaaa tcactgaaga tgtatgatgc
    24421 aaaatacaaa gagttcatcg aagaatactt tccttctttc gactacgcca aagtgaatcg
    24481 gctgcttcaa atgaggtcag atatttttcg aattcttgca gagagtgcaa tagatgcaga
    24541 tgtgaataaa tatttttcgg atcggttaga agatatcttg caacgttcgt atgtatcaaa
    24601 cgcaaacgta tttgcccaga ttttgaacgt agatttgccg aattacttat caaaggaaga
    24661 acttgaacgt tacctgaact atccttggtc aggtaagaca ttctcgcgtc gtttatgggg
    24721 caatatttca tctctagaac aaaagctatc taacgccatt acaactagtg tagcaagtgg
    24781 cgaaggagtt ctcgtggcat tacgaacaat gcgagaagac ccagaaatat gcgatatgtt
    24841 caaaagggag gaatcgaagt acaacaaggc gatcgaaaat cttgtgagaa cagaatacgc
    24901 taaattcgct caagatggta ttgagaaatc ttatattgaa actggcgtag acgaatacaa
    24961 tgtcttaact gcaaaagatg aaaaggtatg ttcaatatgc ggtggcaaag caaagaacaa
    25021 cccatacaag ttgagtgaag cgatcattgg tgagaaccgt gcgccttttc atggccgatg
    25081 cagatgtaca gatgttccta atatgccgaa attaggaaaa gatattgacg aagagtatga
    25141 gcgtttattt ggcgatttat tagatgagtt tgcacatgat tcttttggaa ttaatttgaa
    25201 acggaggaag tagaatgaaa gatttttttg aagcagtact aacaattaat gtaaatgctg
    25261 atattgcaga agcctacaaa acagctattg agtctgagaa ccatcctaat ggcttgagag
    25321 accattggaa tggcaattat gcctacgtgg ttattggcga tcaaactgtt aattatcaag
    25381 ataatactcc agttgataag aataccgtta atttaacgat tcaattatta tctcattcat
    25441 taccaaattt aaaagaaaca gttgattggt atgaaaatat gggatgtatt gttgttagaa
    25501 ctgactacaa agaaggaaag tctagtaatt aggctttttt attttgtccg aaatgacgtt
    25561 aaactagcgc aatactgggc ttggttgaat ggtggggcgc aactattaaa actcaaagca
    25621 atgcggggcg tgaaaacgaa tcgtggggcg aaaggagaat gattatgaaa cacaaatcat
    25681 taatgccaat gaatttgcaa tactttgccg aaggtgacga tcaaaagttt tcttttgatg
    25741 acttcaaatc ttttgtggaa tccaatgaag aagctcaaaa atttattcag tcacaatctc
    25801 aaagtgttgc cgacaaacaa ttggaagctt ggaaacaaaa taacttagat aaaattaaac
    25861 aggataccat caaggaatat gaggaatcta agaaaaacaa gtcacctgag caaattcaac
    25921 tggaaaaact acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata
    25981 aagcatttgt tgcagaacaa attgctggat tagaactaga tggagagtta aaagagtcta
    26041 tttctcaatt tatgctaaat aatcttgtta gttcggatac agatttcact aagaatgctg
    26101 ttgaaggttt tacaagtgtt ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag
    26161 aactacaaat gaagtctgca tttggtggaa ctcaacaatc gaataaccag gttcagcaga
    26221 acaatgaaac attaacaaat ccagaagaac aattaggaca aattcttcaa caatttaact
    26281 aggagagtga aaaattatga aaaaaacatc tttaaataat ttagagtatt tggatatttc
    26341 accagcgatt aatgctatgc aagtaccaaa tacacctttt ttaagctatt tacttggtgc
    26401 tggaaaaaca gagccagcaa actcgacaga aattaaatgg cgtgaatatg atatcaacaa
    26461 cgatgattct tctgaaaaac ttgagggcgg agaatatcca gatgctgaat caggtcgaac
    26521 ttggtttaac aactatactg aaatttttag aaaatcaacc tctgtatctg gtacattaga
    26581 tgctattaat gtgaatggag tcggaaatga attaactaat caagtagcac tacgtggtat
    26641 ggaaatgaaa attgatttga accgaaaatt gattactggt gtaaaagctg atgaaaatgg
    26701 ttctaaaggt cgtcgaatga atgggatttt gaacttgatc aattcagcaa ataaggcaga
    26761 aacagccact gcgggtgcag taacaagaaa agatatcgat actttattta aattgatgta
    26821 tgaaaaaggt tatatgggag aaaaactatg cttgatttct ccagatatgc aggagttaat
    26881 gactgatgag ttagatggaa aatcaacaaa aattgttcag ttcggggaaa gagtaacttt
    26941 tggattgcaa attggaaata tcgtgtctaa ttacggtaca ggtattgctc tactagaacc
    27001 atcattgcca aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac tacgtccatt
    27061 acgtgaatgg agagcagaag aacttgcaaa aacaacggat tctagacgta tcggtcttgt
    27121 aggtgaatac tctcttgaat acaatgcttc aaactctggg gcaattttaa atttaaagtc
    27181 tgaataaaag ggagttagta ctcccttttt tgataggagg aattacagtg gtaaaaaaag
    27241 atgaaactaa aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa aattttgttg
    27301 ggtttgttca tcctaaaact cgtagattta tcacagcaga ttcaaataac gaatttatca
    27361 tttctataga tgataaagaa gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa
    27421 tttaggaagt gatctgatgg atgaatcgct aaaaacggaa atcattgagt ctacaaaaga
    27481 agattttcca gatttgagtg aagaacgcat aactaattta ttagaaataa ttttgctaga
    27541 aattgaatca tacaacactt gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat
    27601 tagtgaagtg ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat cttcagttag
    27661 acgtggcgat acaacgatta gttatgcttc aacaacaaat gatgttagtg agttacttct
    27721 aggctatggt gatttgatac gaagagttat tggttgtgga ggattggagt ttttttaatg
    27781 aatgaagcag atattttaga aactacttat gaagatagtt gtattattga aagactgacg
    27841 gacattgaag attctaatac aaatattact attcaagatt acaaaaaagt atatgataat
    27901 cctatttctt gcgctctttc acaaggtcaa atcgatggac tagcagtcat agaagatgga
    27961 gagatggtaa atgtttcaac tgacacatat aaattatttg ttcatcctaa gattaaacta
    28021 aaaaaaggag atcgaataac aataactcaa aaggccagtg gcttaatttt ttctctattt
    28081 gctactaagc ctttttacta tcctagtcat tgtgaagtga atttgatagg aagtgagaaa
    28141 aatgggtaat ctcaaatatg aatcaaatgc agaaaagatc attgagaatt ttaaaaatat
    28201 gactgtaatt gctcaaaaag aaggaatatc cttcgttaac gattcaatga ataaagtcgt
    28261 tagtttagct aaaccattaa ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt
    28321 agtaaaagct agaaaactat caagcggtcg aattgttgga gcagttataa acaatgaaca
    28381 ttattttaaa tatgtagaag aaggtcatag gactaaaaat ggtggctttg tcaaagggaa
    28441 attcatgtta actcgtgcaa cgaatcttgc aaatatgtct tatattcctc gaagatttaa
    28501 acaaatggca ataaaaatcg ttaagaaagg aaagtaacat gaaagatgaa atcattgctg
    28561 caatcagtag caaattaaaa gaaatctatc ctgatgggac aatatatctt gattcagtta
    28621 tgcagtcaac taaagatttt tatttcgttt tatcagtaat ggaatctgga actgaaaatg
    28681 taggaattga tattcaaaat gtttctttct taattgatat tgcattgatt gataataaac
    28741 ctaatagaaa tttaattaat gaattagtct cacgttgtgg gacttttttt aatacgatta
    28801 caatcgatga gcaaatatta tttccaaaag cctatttacc tgatgaagca gatggtgttc
    28861 aacatattcg ttttacatta gaatttccac aatatattga atggagtgaa agataaatgg
    28921 gagaaaaaag aagtaaaacc ggaattattt ctgtcgaaaa acctacttgg tttcctttgg
    28981 aagatgagac agggaatttc ccggtatatg gtactgcgtt gccaatggga acagcagtaa
    29041 gtatcaaacc aacagctaat tatgaaacaa cacaagatta tggggattca gtggttcaag
    29101 atcaatttac agcttttggt ggtgcagaag ttgaactgga agcaaatgga tatactcata
    29161 aagttttatc tacaattact ggagggaaaa ttgttaaagg tggtgccttg cgttctggag
    29221 aagatattgc gcaagatggt gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc
    29281 gttatacggt cttttataaa ggacagtttg ctctagattc agatgaaacg tcaacgattg
    29341 aaggaagtaa agtaagtttt actcatccaa cttggaaggg ttcatttgta gatgtaccag
    29401 gacttggata tatgtattca gttgatgaag atgatgaagg cgtggataaa gcaatgatcg
    29461 gaaattggtt tacaaaagta gcaattccaa ttgaagaagc agaactttca ggaggtacag
    29521 aataatgtca aaatatcaaa caacgattaa attaacgaaa aaagatgaag aaggtaaata
    29581 tgagcaagta caatttaaat ctgctgaatt tttaccagga acagttgtag aagatgctgc
    29641 aggagttatg gaagaaatgc aaacagcgac tgataaacaa tcagttaaaa aagctttaag
    29701 tcgtgcttat tcatttattg cagatacttt atttgaggga caatttactg gtgaagatta
    29761 ttgcaaagga attgatgctc gtgagattgc ctcattgaca ggaaaattat tgaagtctgt
    29821 tactgcaggt tttgatgaaa cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag
    29881 tcaccttcat ttaagtattc gattacttac cgagaattag atataaagac gcaattactc
    29941 gaagcaggtt ggacgttacc agaaattgaa cgtactgact tggatgagtt aatgcgtctt
    30001 tttgctttta gagatgcggt aaaagagcat gaagatgttg agtactatga taatttcact
    30061 caattttagg aggtgatact ttgaataacg atgacctaat tctgaagatg atactagatg
    30121 aatctggttt tactgccggt atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa
    30181 caatcgaaag aaaaagtaga aatggtggca gttcattagg tagtatttgg aaaatatttg
    30241 ctggaagttt tctagctagc ggagtaacta gaatcgtagg agctggcttt gatctaatca
    30301 agggttccat aagtggagcg gttgatcgag tagatacgat gaataacgct ctacgaaatt
    30361 tccaaaatat gggatttagt aattcagaaa ttatgaagaa tatcggaaag aatgggcttt
    30421 tatctcaagg tattcaagga cttcctactg ctttgaatga tgcgataagt catgttcaac
    30481 ttcttgcttc ttctacaggt gatttaactc gttcaactca aatatttaaa gctttaaatg
    30541 atggaattct tggtttcggt ggttcaactg atcaagttaa tgaagctgtt attcaattat
    30601 ctcaaagttt ctcaaacgga aaagtagacg cacaaacttg gaactcaatg atcaacgctc
    30661 aacttggacc tactctttct gctattgcta aaaagatggg aattacaatg ggagatctga
    30721 aagaaggttt atctcaaggt aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa
    30781 tggataccaa aggtggcgga ggacttaaat cattaagtca aatcgctaaa gattctacta
    30841 agggaattaa aacctctata caaaatgcaa aaacagctgt tacacgtggt gttggtgagg
    30901 taatagaagg attaaataaa gcattagtag attcagattt aggtggattt aaggggatta
    30961 ttgataaagt tgccagttcc atggaatctt ttttgaaagt aattgctgca aatattccta
    31021 gagcagtgtc ttttttaagt aatctttttg atgcagttca aaaatttggc tctgcattga
    31081 aattcatgat gccatttctg gttcctgcaa ctactgcttt tggcgcatta atgtttcaac
    31141 ttaaaggaat accaacaatt ataaaaagct tcaataattt taagaatgcc ataatcggtg
    31201 ttggaaattc actaaagatt atgggggcta tagccgccgc aaatccgttt gttttgattg
    31261 ttggagccgt tgttggagcg attgctgtat ttggctattt tatggcaacc aacgaagagg
    31321 ttagaaacaa agttatatcc gtttggaatg atgtaaaaga ttccgtactt ggtgtattaa
    31381 agaatataaa agattgggga attgatactt ggaattctgc taaagaaatg gcatcaaatg
    31441 ctgttgagag tgtcaaggat gcttggtctg gaataaagga atggttttca aatacttggc
    31501 aaggcattaa aaatggagca acaggtttat ttgataaaac agtagaaaca tctatgaatg
    31561 cggttgatag tgtaaaaaac gcatggtcaa atacaaagca atggttttct gatatttggc
    31621 agagcataaa agattcagca actgaaaaat ggaatgaaat taaaggttct atcatggaag
    31681 ttgctggtcc attgattaca ggtttaaaga acgcattctt gcatgtcact ttttatttag
    31741 agaccttatg gaataatcta gttgagattg gtaaaaatgt ttttgagatt ttaaaaaatg
    31801 ttatacttac gccagttttg tttattacct ccatgatttc tggtggatgg gaagaaacaa
    31861 aaaacaacat gattggtgta tggaataata ttaaagaaag tgctctaaat atttgggagt
    31921 ctataaaaaa tatttttgtt agttacatta caaatattta ttttgctgcg cttaatattt
    31981 ggacagggtt caaacttact ttgataaaca tttggaatga agtagtaagt caagctaaat
    32041 caatttggat taatgtaaaa tactttttta ttaatctttg gattgacatt aagtattttg
    32101 ctattcaaaa atggattgaa ttaaaattcg ggattattca aacttggatt gatttaaaat
    32161 ataatgctat tactacttgg aacaatatta aacagttctt caaagatact tggcagaaca
    32221 ttaaagatat agcatacaat acatggattt ctataaagaa ttccatgatc aatacttgga
    32281 ataacatcaa ggaatctttc tggaatattg ttactggaat tgttaactcc gctgaaaatg
    32341 catggactaa tctaaaaaat ggtgtttcaa aagcaattaa tcgggtgaaa gaaatctttg
    32401 attcattaag ggaaatcaat ttattcgaaa tcggtaagaa tatcattgat ggacttatca
    32461 atggtgtagt agaaaaatgg aatgcattga aaaagactat taaaggaata gctggaagta
    32521 tcaaagattc tattaagggt gcattaggta ttcattctcc atctagatgg atgagggata
    32581 tggtaggtaa aaatattgtt caaggtatta tcgttggtat tgataaagaa aaaagtaaat
    32641 tggatcagac aatgactgat ttagtgaaaa ctccgtcggt tcaaccagtt attacagggt
    32701 ctaatagtca accagtggta caagctaaac aaaatacatc ttcaaatgca gtgaatgaaa
    32761 ttcacttgca tttaaatgtt tatggagatc tacctgattc gatgattaaa caaatcgcca
    32821 agaagatgaa aacagaattg acgagacaaa tgaaacgaga tgctgatgca gtaggaggga
    32881 cattatatgc aacttaaaag aggacaattt tttatcaatc aacattattc ttctgagttt
    32941 aacgtatata ttcaaaatag gcctgcctct gtttcagcta gtcgtgtaat tgaattgaga
    33001 gaacgtgaag gcaatgactc aattattatc gataaagcct attacaaaaa cgttactaga
    33061 aaaattgaat gttattacaa agcaccatcg attgatttag tgcaggaatg ggaagatcga
    33121 attactgaat ggttagatat gaactcttac agtgatttta ttctttatta cgatcaacaa
    33181 tatatctatc aagctatagt aaccgaagcg ccagaattta aaggaacaag aaaaacaggg
    33241 aacatagttc catttgaatt tacagttagt cttcgaccat tcaaagaaaa ttatagtggt
    33301 cgttttgcta ttcaacagat aaaagctttc gagttattta atccagagaa gtatgcttca
    33361 aaaccgctta ttaaattgag tggttctgga gatgcttctt tttatattaa taatgataaa
    33421 tatgatttga aactattaga tagagaatta tatatagatt ctaaactaga agagtcttat
    33481 cgaaagctag atgacaattt agaacatcaa gatcatgtca ctttattttt agattttcca
    33541 tttctatacc ctggaaaaaa tgaaatcaaa tggacgaata acatacattc gtttgagata
    33601 ataccaaggt ggtggagaaa agtatgatac caagaatata tagtcctacc gagacagatt
    33661 tttctacgaa tggtttaggg attttgaagg acaccacaaa atgtgagata tatgaagtag
    33721 caaatggaaa atatgaatta gagttggagt atccattagg tactcgattt gatgaatatt
    33781 ttgaaaatga ctatcaaata aaagcaaagt caaatgatca agaagagtat catatctttt
    33841 ttattgatga taaagatatt gatacctttt tagataccgt aactatttat gcccagagtc
    33901 gtacaaatcg gcttggaaga cgagcggtca cttttgctgg agtcgactct aaaactggtc
    33961 gagaagcaat ggcaattatt gaaaacaata tggataaaaa atcagatata cgcttatatt
    34021 ctgatattac aactgtatca agtacaactt ttgaagcaag aaatgtttta aattgtattg
    34081 ctggtgaaca aggatcttta cttcagtatt ggggcggaga aattaaacgt gagcctttta
    34141 agctatcttt attaaagcga agaggtcgtg ataatattgg cacaatccga tatggaaaag
    34201 atttatctgg gttaaaagtt aagttagatt ggacaggcgt gaaaacaaga attattccgt
    34261 atgctgatcc tcaaagtgat gcaggtacaa ctagccgaat ttatggctcg ccagtagata
    34321 gcgaatatat taataattat cctgatgtat atacagaaca cgttcaattc acagaagaac
    34381 agggagttaa agacgttaat agcttaaata aaatagctaa gaattatttc aaaacaatta
    34441 atccaggctg tgataaaccg aagatttcta ttaccgttga gttcgataaa ttaacggaca
    34501 ccgaagaagg aaaagaattc gctaagataa gaaattatgg gttatttgat acgtttaaaa
    34561 tatatcatcg aaaatataaa ctatattttg aatcgaaagt tagcggtgtt caatatgatt
    34621 cattatctga aaaggttttg aaattggaag ctggggatgc tcaagttgct ttttatcaac
    34681 aacaagctgt tactattcaa gataaattaa aagattatgc tacgaataat tatatgagta
    34741 gttttaatga ttatgtgtcc tctatgattg caggtcaagg aaatgcaggc ggttatgtag
    34801 ttttatggcc aaaagaaaaa ccttcaaata tttttataat ggatagccct gatttaaaca
    34861 aggcaaaaga agtattaaga atgaacaaaa atggaattgc cttttctaaa aatggttgga
    34921 atggtccttt taattccgct tggacattgg atagtatatt caatgctaat tttattcaaa
    34981 caggattaat aaaggcagat atctttcaaa actcttttaa taaaactgga gatgtattga
    35041 aattagttaa tggactactt caaatttgga ataacaaaaa gaaaataatg gagttaacca
    35101 aaaaaggaat ggagttttgg aattctaata gttcaattgg aacgattgga acaactgatt
    35161 ctgctggtaa tccttttcct ggagcttcta ctcccacacc tattgaagat aattctttag
    35221 ttattcgtac aaatggagac ggcaaatata ttttgatttc tcctaaagtt ggtaaaggat
    35281 tagttttatt aggaaatggt aaagcaattt attttggaga cttagatgta caaggcaaac
    35341 tcacagttaa cggaaaagaa atcacaggga acaacagtgg aggaagtgat cctggaacta
    35401 tccctcctca attgacgacg gaagctgaaa aaagagcatg gaaaatttgg acaatgctga
    35461 aagctcgtgg ctattctgaa tatgcagctg caggtattct aggaaatatt cagggggagg
    35521 ttggagcaag tatgaaccct gatacagaac aacttggtgg tccagcttat gggattgttc
    35581 aatgggatgg ttctgcttat ccgttagtcg gatcaccaac ttggaacgga cgagagtatg
    35641 ttcagcgttt gatgaacacc gcagggattc aagaagatta tcgaagtatt gaagcccaag
    35701 taaaattatt agattggtgt atgttcaacg gtcaatggct cggaaaagta aatccaacca
    35761 cagtatcagg atttaaatcg atcaatgatg ccaaaagtgc agcgtatgct tttgaaatga
    35821 acttcgaacg cccagcttct gcacatccag aacgccaaaa ttatgcccaa tcttggtata
    35881 acaaattaca tggattaact agtccagaac ctggagggaa tttcatttgt ccaattcaaa
    35941 aaccagtgac agttacttca gaatgtggat ggagaactag tccaataaat ggcggccaag
    36001 aatttcataa tggaattgat cttgtaaatg gaaatcctaa tacacctgtt tttgcagcat
    36061 tagatgggga agttgttcag gctggtgcta attattatga ctggtatggt aattacgtgg
    36121 ttattaaaca taataatggg aagtggacag gttatgctca tttgtctcgt attgatgttt
    36181 ctgttggaca aaaagtccag aaaggtgctc aaataggctt gatgggaaca actggtccat
    36241 ctacaggaga acatctacat tttcaaatta tgaaaaatta ttggccacag ccagttgttg
    36301 attttgagaa tccaagaaat tatatccaat tttaaggtgg tgattctatg agtaaatgga
    36361 atgtcgtttt aagtacaaca gaaccatata attatgtggg gatgattcaa gttcgacaag
    36421 gcaataagaa tacagaggtt atggaagcga ctatagttga aaatggtctt ccctacgatt
    36481 tatcagaatg taaggtatat tttgaatcag ttgtaggtgg gaaatatcca gtccaattag
    36541 aaacaaaaat tgtggatgct aaaaaaggga aaattaacta tatttttgat aaatattcca
    36601 tgcagtgttt gcatcgacaa acagccaatt tcattatatt taaaggagaa gacttgattg
    36661 gaacaactca agacttctcc tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa
    36721 tgggttctta ttggcaatca attgaagatt taattgctga tatgacagat tttattaatg
    36781 aaaataaagg cgattttacg gactggatga atgaaaggaa agaagaattt gatcgctgga
    36841 gagaagaaca ggagaatagt ttccaagatt ggagagaagg acaagaatca gattatttgt
    36901 catggtttga atcaatcaag gatattttga agtctatcga tccaggtgga gtaatgttag
    36961 ccgaattaat ggatgcacgt gttgatattc aaggagttcg tcatgcgtct atttcagagc
    37021 gcttgctggc agatatggat tatctatatc agaaattacg agcaacactt ttcacgattg
    37081 aatacggtga aattgaagtg actgatattc ttcaagacga tctcttttca gataatcacg
    37141 aagtcgaaaa aattgaa
//
LOCUS       DACEKQ010000017        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEKQ010000017 DACEKQ010000000
VERSION     DACEKQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.017"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW671_000784"
     CDS             <1..1152
                     /locus_tag="IW671_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897793.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW671_000785"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW671_000785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0897794.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW671_000786"
     CDS             2728..3003
                     /locus_tag="IW671_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0897795.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW671_000787"
     CDS             3009..3776
                     /locus_tag="IW671_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0897796.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRIYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW671_000788"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW671_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0897797.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW671_000789"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW671_000789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0897798.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW671_000790"
     CDS             7920..8222
                     /locus_tag="IW671_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0897799.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW671_000791"
     CDS             8225..9388
                     /locus_tag="IW671_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0897800.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW671_000792"
     CDS             9414..12842
                     /locus_tag="IW671_000792"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0897801.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW671_000793"
     CDS             12863..13987
                     /locus_tag="IW671_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897802.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW671_000794"
     CDS             13977..14414
                     /locus_tag="IW671_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0897803.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW671_000795"
     CDS             14398..14736
                     /locus_tag="IW671_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0897804.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW671_000796"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW671_000796"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0897805.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW671_000797"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW671_000797"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0897806.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW671_000798"
     CDS             15893..16936
                     /locus_tag="IW671_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0897807.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW671_000799"
     CDS             17095..17823
                     /locus_tag="IW671_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0897808.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW671_000800"
     CDS             17939..18184
                     /locus_tag="IW671_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0897809.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW671_000801"
     CDS             18181..18399
                     /locus_tag="IW671_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0897810.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW671_000802"
     CDS             18535..19074
                     /locus_tag="IW671_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0897811.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW671_000803"
     CDS             19067..21340
                     /locus_tag="IW671_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0897812.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW671_000804"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW671_000804"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0897813.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW671_000805"
     CDS             complement(22513..22767)
                     /locus_tag="IW671_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0897814.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW671_000806"
     CDS             complement(23167..23436)
                     /locus_tag="IW671_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897815.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW671_000807"
     CDS             complement(23906..24361)
                     /locus_tag="IW671_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0897816.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW671_000808"
     CDS             complement(24499..26427)
                     /locus_tag="IW671_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0897817.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW671_000809"
     CDS             complement(26624..27457)
                     /locus_tag="IW671_000809"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0897818.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW671_000810"
     CDS             complement(27649..28353)
                     /locus_tag="IW671_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0897819.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW671_000811"
     CDS             complement(28376..29002)
                     /locus_tag="IW671_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0897820.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW671_000812"
     CDS             29328..31703
                     /locus_tag="IW671_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0897821.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW671_000813"
     CDS             complement(31727..32218)
                     /locus_tag="IW671_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0897822.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW671_000814"
     CDS             32405..33325
                     /locus_tag="IW671_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0897823.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW671_000815"
     CDS             33479..33823
                     /locus_tag="IW671_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0897824.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW671_000816"
     CDS             complement(34013..34513)
                     /locus_tag="IW671_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897825.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW671_000817"
     tRNA            35041..35124
                     /locus_tag="IW671_000817"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11777 a         6169 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tatacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtaga
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEKQ010000018        35175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEKQ010000018 DACEKQ010000000
VERSION     DACEKQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.018"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            162..272
                     /locus_tag="IW671_000818"
                     /pseudo
     CDS             162..272
                     /locus_tag="IW671_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(369..845)
                     /locus_tag="IW671_000819"
     CDS             complement(369..845)
                     /locus_tag="IW671_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0897826.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(858..2360)
                     /locus_tag="IW671_000820"
     CDS             complement(858..2360)
                     /locus_tag="IW671_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0897827.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            complement(2374..3063)
                     /locus_tag="IW671_000821"
     CDS             complement(2374..3063)
                     /locus_tag="IW671_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0897828.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3224..4042
                     /locus_tag="IW671_000822"
     CDS             3224..4042
                     /locus_tag="IW671_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0897829.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4272..4502
                     /locus_tag="IW671_000823"
     CDS             4272..4502
                     /locus_tag="IW671_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0897830.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(4725..4823)
                     /locus_tag="IW671_000824"
                     /pseudo
     CDS             complement(4725..4823)
                     /locus_tag="IW671_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(5088..5543)
                     /gene="tnpA"
                     /locus_tag="IW671_000825"
     CDS             complement(5088..5543)
                     /gene="tnpA"
                     /locus_tag="IW671_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0897831.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            5786..7212
                     /locus_tag="IW671_000826"
     CDS             join(5786..6359,6359..7212)
                     /locus_tag="IW671_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0897832.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            7401..8069
                     /locus_tag="IW671_000827"
     CDS             7401..8069
                     /locus_tag="IW671_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0897833.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(8269..8655)
                     /locus_tag="IW671_000828"
     CDS             complement(8269..8655)
                     /locus_tag="IW671_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897834.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(8627..10009)
                     /locus_tag="IW671_000829"
     CDS             complement(8627..10009)
                     /locus_tag="IW671_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0897835.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(10165..11352)
                     /locus_tag="IW671_000830"
     CDS             complement(10165..11352)
                     /locus_tag="IW671_000830"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0897836.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(11359..12390)
                     /locus_tag="IW671_000831"
     CDS             complement(11359..12390)
                     /locus_tag="IW671_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0897837.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(12406..13860)
                     /locus_tag="IW671_000832"
     CDS             complement(12406..13860)
                     /locus_tag="IW671_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0897838.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            14035..14940
                     /locus_tag="IW671_000833"
     CDS             14035..14940
                     /locus_tag="IW671_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0897839.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            15031..15708
                     /locus_tag="IW671_000834"
     CDS             15031..15708
                     /locus_tag="IW671_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0897840.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            15989..16885
                     /locus_tag="IW671_000835"
     CDS             15989..16885
                     /locus_tag="IW671_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0897841.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(16940..18310)
                     /locus_tag="IW671_000836"
     CDS             complement(16940..18310)
                     /locus_tag="IW671_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897842.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIR
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            18548..19294
                     /locus_tag="IW671_000837"
     CDS             18548..19294
                     /locus_tag="IW671_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897843.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19291..20130
                     /locus_tag="IW671_000838"
     CDS             19291..20130
                     /locus_tag="IW671_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0897844.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            20131..21081
                     /locus_tag="IW671_000839"
     CDS             20131..21081
                     /locus_tag="IW671_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897845.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            21222..21974
                     /locus_tag="IW671_000840"
     CDS             21222..21974
                     /locus_tag="IW671_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897846.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            21993..23144
                     /gene="queG"
                     /locus_tag="IW671_000841"
     CDS             21993..23144
                     /gene="queG"
                     /locus_tag="IW671_000841"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0897847.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            23529..24137
                     /locus_tag="IW671_000842"
     CDS             23529..24137
                     /locus_tag="IW671_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0897848.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            24305..25942
                     /locus_tag="IW671_000843"
     CDS             24305..25942
                     /locus_tag="IW671_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0897849.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(26014..27126)
                     /locus_tag="IW671_000844"
     CDS             complement(26014..27126)
                     /locus_tag="IW671_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0897850.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(27201..28574)
                     /locus_tag="IW671_000845"
     CDS             complement(27201..28574)
                     /locus_tag="IW671_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0897851.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            28795..29412
                     /locus_tag="IW671_000846"
     CDS             28795..29412
                     /locus_tag="IW671_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897852.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            29510..29965
                     /gene="argR"
                     /locus_tag="IW671_000847"
     CDS             29510..29965
                     /gene="argR"
                     /locus_tag="IW671_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0897853.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            29983..31485
                     /locus_tag="IW671_000848"
     CDS             29983..31485
                     /locus_tag="IW671_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0897854.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            31580..31786
                     /locus_tag="IW671_000849"
     CDS             31580..31786
                     /locus_tag="IW671_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897855.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            31872..32273
                     /locus_tag="IW671_000850"
     CDS             31872..32273
                     /locus_tag="IW671_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0897856.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            32428..33759
                     /locus_tag="IW671_000851"
     CDS             32428..33759
                     /locus_tag="IW671_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0897857.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            33781..34407
                     /locus_tag="IW671_000852"
     CDS             33781..34407
                     /locus_tag="IW671_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0897858.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            34590..35171
                     /locus_tag="IW671_000853"
     CDS             34590..35171
                     /locus_tag="IW671_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0897859.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
BASE COUNT        11754 a         6443 c         6861 g        10117 t
ORIGIN      
        1 acatctttca gcattttact atatttggta tatagtgcta aggttcatat tcgattgaca
       61 agagaatata aattgatcta tttctatttc ctaagttgag taaacataac tttgcccttt
      121 ataaatatct tccctctctc tcttctctct atattttata tttgaataat catatcaagg
      181 tattccaacg aaatgccaat ggatttcgaa gcttttataa ctttaaatta cggatcatga
      241 tttggcatgg ggaatcatta ctaataaaat aaaaaaatga acagaaagaa aacagttctt
      301 tctgttcacc aacttcattt gacaaatatc ctatttagct tattttatag ctttttatat
      361 acactttttt aaaaagtgat ttttccacat gaattcccat gagcaattcg accattatct
      421 attattaaat cattgaactg atttttctcc ggaaaaacaa ctataacaga agtatcttta
      481 ccagatttta ttattttgtc aaaatccaca tcaaccaacg aggtttcagg agtaacctca
      541 tcattttctt gaacacaaat cgaaaaaggc tctccttgta attcaactgt atcaattcca
      601 atatgaatca aataatcaat gcctgagttg ctttttatat taatagcatg tttagtagga
      661 aatatatttg ttactcgacc agccaatgga gcataaattt ttgaaccatc aggtatgacg
      721 gcaaaaccat cacctagtaa tttttgtgaa aagacaggat catttacttc tgtgatggaa
      781 atgatttttc cggaacaagg tgcataaaaa gtttcttgtt ttttcttttt gaaaatttca
      841 aacatattaa aaacctccta attatctttt cctaatattt gattgatttc ttgacttaat
      901 aagtcagctt tccctccaaa aacagcttgg atgccaccct tcacttcgaa gacagcagtt
      961 gcaccagtgt cttttattgt ttgtttttga ataatctccc cattcgtaac agcaacacgc
     1021 aaacgagttg cacaagcttc aacagactca atattttctt ttccaccaag agcttcaata
     1081 atgataagag actcttcatg taatgatgta tttttgttta ttactttttc agaatgaact
     1141 tctgattctt cttccattcc aggaatagct accttaaatt ttttgataaa gaatgtaaaa
     1201 acaaagaaat atataaatgc ccatgcaata ccaaaaggaa ttaattttaa ccaattagtt
     1261 ttgtcattcc cttgaagtac accgaataat aaaaagtcga tcaaaccacc tgaaaaggaa
     1321 ttccctacac gaatatttaa gatatcagca aaataaaaag ataatccatc taagaatgca
     1381 tgaatcacat aaagccaagg agcaacaaat aaaaacatat attcaactgg ttcagtaatt
     1441 ccagttaaaa atgatgtaag ggccccgcta aaataaagcc caccattctt tttacgattt
     1501 ttcttaggaa ttgcacgata cattgcataa catgctgctg gtaatccaaa catcattgtt
     1561 gcaaatcttc ctgcaaaaaa tctagtacca taagtaaaca gaccagtgtg gtttggatca
     1621 gctagttggg caaagaaaat attttgggct ccagcaattg ttgatccagc tacagtttcg
     1681 gtaccaccta aagatgtata ccagaacaaa gggtaaatgg tgtggtgtag tccaacagca
     1741 cctgttaaac gcaataaaaa accatataaa aaagttccta aactacccat ttgggcaatt
     1801 tgttcaccag caactactaa accattttga attggtggcc aaattaaata aaagaaagaa
     1861 ccaataatga ttgcagctaa actagaaaca atcggaataa atcttgatcc tccaaaaaaa
     1921 cctaagaatt gtggtaattc aatttttcta taacgattgt gtaaaaatgc aacagtacca
     1981 ccaacaacaa ttgatcccac tacacctgta tctagtgttg catcttttgc tgaaaaaagt
     2041 tcaagaaacc cactaatagt tgcagtatat actagataag ctactccacc agctaaacca
     2101 gctgttcctt tatctccatt tgcaagtcct acagcaatac caattgcaaa aattagtgct
     2161 aggtttgcaa aaactgcatt tccagcataa ctcataatac ttaaaattgt atgcaaccac
     2221 ggttgatcta aaaatggata agcatttata gcactctcat ttgttaacgc tcctcccagt
     2281 cccaatagta atccggctac tggtaatata gcaattggta acatgaaagc gcgtcctaat
     2341 tgtgaaaatt ttttaaacat tttatttctc cccctaactt aatgctttaa caaatcttga
     2401 tgcaatttcc tgtggtcgag taatagcacc acccacaaca atacctgcaa ctcctatttt
     2461 ttttatctct tttgcttgtt ctggagtatg aatccttccc tcagcaatta cacaaatccc
     2521 ttcttctacc aattttttaa tcaaatctat atctggccca tcttgttttt tactttcctc
     2581 agtatatcca cttaaagtag ttcctacaaa atctattcct gctttatatg catttttgcc
     2641 ttcttcaaat gtagaaatat ctgccataaa taactgatga ggatattttt cttttatttt
     2701 agtaataaaa gaatttatag atagtccatc atgtcgattt ctaagagtgc aatcaagagc
     2761 gattacctca acaccggttt ctactaattc atctacttct ctcatagtgg cagtaatgaa
     2821 tggagattct ggtggataat ctcttttaat aattccaatc attggtaatg atacacactt
     2881 tctaatttca ataatatctc gaacagaatt tgcacgtata ccaactgctc cagccctttg
     2941 agcagccaaa gcaaaataag gcattaaact ttcttcctct ttgtaaaatg gttcaccagg
     3001 taatgcttga caggaaacaa tcaaaccttt tgaaataaga tctaaaaacc tctttttatc
     3061 cattatccta aacactccaa ttttttatta tttttggata acaactccaa aaacaatata
     3121 gcatccgctt tcataaaagt aaagataaaa atggagtatt taggagtttt ttttattatt
     3181 ttggagtata atggagaaaa gttgaaattg tagaggagtt tgtatgagtt atatctattt
     3241 aattaaagaa aattatccat ttttaacgaa gtctgaaaaa aaagtcgctg attttatttt
     3301 aaattctggg aaaactatta tttatagtac gatgagtgat attaagaaaa acacaaaagt
     3361 tggcgatgct actattattc gcttttgtca aaaaattggt ttttctggtt tttctgattt
     3421 aaaaattgaa attgcaaagg aagactttag tcaaaaaaaa gaacatccga ctagtgtaaa
     3481 gtactatgat gaaattgcta atagtttaac agaagctctc tattccacta ttaggctttt
     3541 agacgaagaa aaattgaatg aagccattaa aatgatcact caatcaaaaa acatatatat
     3601 atttggagtg ggatctagtg gaaatactag tcttgattta gaaaatatgt ttcttcgcgt
     3661 aggagttcaa gcaaaagctg tactagaccc tcattttcaa gcccaagtcg cttctttatt
     3721 aacagttaat gatttagtaa tcattttttc tttatctggg aaaacgaaag atacttatga
     3781 ttcattgaaa atagctaaaa aaaatggcgc aaaaattatt gctataacta actatattca
     3841 ttcacctata ggaaagtctg cagacttagt tttacaaaca gccattgaag aatttcttaa
     3901 tggtggatct ttagctggaa aaatatcaca actttatatt tgtgatctat tagttcatgg
     3961 ttatgaacaa aacaataata ttgattcagt tgaattaaga gaaaaagttt tgcgctctat
     4021 tattgataaa agtattgagt gaactacccc cacttagcta aacctaacgg tttttaacga
     4081 ttgaagtagg caattcttgg gatgagagca tacttgatag cttatttttt actaatagcg
     4141 gtggctctta ttgaccaaat atccctgtag ttcctacggt tcttgatttt atctaaagat
     4201 atttttatcg tggaagctat tgatcaactt gaaagaaaaa cgtgtagctc attatcacta
     4261 gtttatcgag tatggatgaa gcaatggatc atcctctctt aaataaattt attaaggatc
     4321 tgatccttca aattttttcc atgatcgatg agcaggaacc tactgaatct aaacgacatc
     4381 gggcacaagg aattattaag attgctaagc gaatgttttc tataaagggt gttctaagct
     4441 atacagtgtt aattcaaaag attcccaatc atcttgttta taagaacaac gttgaagatt
     4501 aaaaaagtgt ttcaatgcta aaattgcaaa atacttcaat gttatgagac aaactgtcta
     4561 taagaatcaa tttccaccca tttcatcact tgttaaaagt ttatgatcct tttgattttt
     4621 atattaattt atctgtattt aatacagatt ttatttatta gttcatctgc ctattgtatt
     4681 ttattttgtt gccttaccat ctgaagtaaa tatactctta attgtcaaat taagttagac
     4741 agatcatcaa tacttacgta actcaaaaat acttccaaag gtgttcgata atccagtgat
     4801 tttctaggaa gattatttct gcgccagcat tttctaatgt ttgaatttgt gttttgtaat
     4861 cttggttgct tgtgctaatt cttgaatagc cgtaaattgc caaaatttca tcaccctttc
     4921 tggacataag tttctgacac cagttaaaat caagtctatc agtaacccat acacccgtga
     4981 ggacgggtgt atttttaatc cccctagaag gggtcgacac tcgctgcccc ttaaaagggg
     5041 atttttaaaa gaccgccaac tgctcatctt cactcactta cttctcttta ttttttaaat
     5101 ggttctacgt actctctttt acttataata tatcgaatgc gatcatcagc ttcttgttca
     5161 cggatgtatt tttcatttgt cttttgattc aatccgactg tactcacata gtatctcttc
     5221 gaccaaaagg atttgtttcc gtatttatat ttgagatttg cgtgctgctc gtggattagt
     5281 accgcactac gacctttcag ataccccata aaactggata tacatatttt tggtggtact
     5341 cgaacaagca tgtgaatatg atctagcatt gcatgtgctt ctatgatttc cacatctttc
     5401 atttcgcaca acttcctaag tatttttcca agatcttttc gtaattgccc atagattact
     5461 tttcgttgat attttggtgt aaaaataata tgatacttac aattccaacg agtatatgct
     5521 aaacttttat cgtcatttga catgaaaaaa ctcctttcga tctgatgagc ggttggtagc
     5581 cactcactag tatatcgaaa ggagttttta tcgctaaaga tacttcttcc acgcgtagaa
     5641 acgcgcggtt ttcttgtttc gcacgcttta cgcacaaaac tgactgaagt cattaagaaa
     5701 ataaaagggt ctaaaacttt taagttttgt accgaccccc aaaagttaga ccaaaaaatc
     5761 taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat
     5821 cgttcatgat tacttggatg gtaaaggcgg aagtgattat ctggcaaaga aatattccat
     5881 taaggctcca tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg
     5941 tctagtaaga aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga
     6001 gttatatcta acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa
     6061 ccctaatttg atcgctagtt ggatgcgtca attccgtgag ggtgggatcg atggactctc
     6121 aaaaacgaaa ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa
     6181 atctacaact aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt
     6241 ccgttccctt caaattgaaa atgccttttt aaaagaattg aggaagctgc gaaaacagga
     6301 agcccaacaa cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt
     6361 aaattagttg agcttctaga aactttaaag ttccctaagg caatctttat gtactggcaa
     6421 aaacgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaagttcgg
     6481 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
     6541 tctatcaata aaaagaaagt tcagcgcctg atcaagaagc taggaataaa agtaacgaca
     6601 tacacacgta aatcacggcg ctacaattct tatcgaggac aagtcggagc agtagccaaa
     6661 aatcggatcc atcgtcgttt ctacaccagt atctgccatc agaaaatcac gacggataca
     6721 actgaattta aataccatga agtggatgcc aaaggcatta tccgtcaaaa gaaattgtat
     6781 ctagatcctt ttatggatat gttcaatagt gagattttat cctatcgtat ctctgaaaag
     6841 cccaatgcat tagcggtaat ggaaggtttg gaggaagcta tccagaccac taacgattgt
     6901 ccctatcgtc gtacgtttca ctcagatcaa ggttgggcgt atcaaatgaa agcttataga
     6961 aacaaattga aggagtacaa aatctttcaa agcatgtctc ggaaagggaa ctgcttagat
     7021 aattcactta tggagaattt ttttggctta ttaaaacaag agatttttca cgggaaagtt
     7081 tataactgtt tcgaggaatt aaaatcagcc atcgatagct atatctatta ctacaataat
     7141 gaacggataa aacagaaatt aaactggcaa agtctcgtcc aatttcgtaa aacaaccgta
     7201 acggttgtgt aaaaatatca ttttagagca taaaaaaaag gagtggattt ctccactcca
     7261 aaaaagtcta acttttgggg gtcactacag tgtcacagtc ctttttttag taaaaggata
     7321 catttcttcc ttgtatattc catgcgtgtt cgtgagaaag catatataat ttaattaaca
     7381 gatagttagg gggagaaaaa atgatttttt caattacaat gaaactggtg ttcgggttga
     7441 tcggtttatt attagtggta cgtttgcttg ggaagaaatc catgtcagaa atcacaccgt
     7501 ttgatttagt ctatacgctt gttttaggtg ggatcttaga agaatcaacc tatgatgaca
     7561 atgtccatgt agggcatgtc ctatttgcca ttgcgctttg ggcagtaatg atttatggta
     7621 tcgaacgtat cgtccaaaaa aatgaaaagg tcaatcgttg ggtcaaaggc gagccatctg
     7681 ttctaatcaa agacggcgtc atcaacatga cagagttgac aaataaccat atcgagatgg
     7741 agcagttgcg agcaatctta agacagcaag aatgcttttt tcttgaaaat gccaaacatg
     7801 ttatcctaga gaatgccggt caaatgagtg tattgaaaaa atcagatgaa gacaaagctt
     7861 tatcgatcct gcttgtagat gaagggcaaa ttcagcataa agtgcttcag tccaatcaat
     7921 tgactgaagc atggcttatg gagaacttga agaaagaagg ctatgcagat gtaaaacagc
     7981 tgatttatgt tgaatggtca gaagaaaaag ggctctacat actaaataaa gaagacgtca
     8041 tgcatgaatc ttaccgaata gatggataga tagacagagg ttgtgaaatc agcgccttgg
     8101 cttgttactc aaaactaagc gactttttca caacctctga tatctaatat acgttgttcc
     8161 agaagttgct tcttcgaaaa ataagccaaa attccacaaa aatttaagaa gcaattttcg
     8221 aaaaatcctt tcttattttt tgaagttgat cacttctgtc acaacctctc atgtcacaaa
     8281 ccattttttt gtttccttta actcaatata ccgtacgtct tcccacaata tcggctgatg
     8341 gtcaatcata aagtaatctt cataggcttc accaatgaag tgcccttcga cctcatccaa
     8401 gagtcttcca aattcatctt ttaaattgag ctgaatgatt actgctttac gagataaaaa
     8461 ggcttctgtc agtatactat cgatttcttg caatgtcatt tgttgtttag gaagaggctg
     8521 tttgcgtgct tcttcttcat tttttttgat accgttgact agttcttcca tcgcaaaagc
     8581 agtcccccat tttaatccaa atggccgatc tcgatactcg ttatagtcaa tgaatttttt
     8641 tgctgtccgt ctcatctttt ccaccttcta atccactcat accacctgca tgcccgccaa
     8701 cgagagaact acgtgcgatg gcgcgtcctc cttgcaagag actatgcgca tggatgattg
     8761 attgaaagcc gtattttttt ctaatcgtat cgacaacaaa atcaactttc gcttcattta
     8821 tctgcttttc aggatcttca aaaaggttaa gctgtaaacc tgtcgcgtat acgaggtcgg
     8881 aacagttcac gcctacacgc cgaatatcct ggtagtggta atgctggtgg aaaatggtca
     8941 ataaagcatc tgctagatct ctgctagcat ttgtcgcttc gattttcatt tgctggtgaa
     9001 acccggtctt gccatttgga tcaagatagc ctaaagagta tcctacccat aagctgacta
     9061 cttgtgtttt tgctgcagat cgtctcaagc gagtgccgac ttggtcggcc atttctttga
     9121 taacgatctc gatttcttct cgtcttgtat aatctctatc taggatttga ctatttccga
     9181 tactttttga ttttggtgta tattcttgtc ccaaaaaact tcgatcgatg ccccaagcat
     9241 gggcataaag ctgagtaccg atgaccccta gcttgctttt caagtgataa taatccgcgt
     9301 gagctaaatc ataaacagaa tgtataccca tttgtttcag acgttttgct gttcgtttgc
     9361 cgattcccca aaagtcagtg atatctgaga ttttccataa cttttctggt acatcttcat
     9421 aacgccattc agcaaacatt tgctttccgt cttttgagcc attgtctaag gctagtttag
     9481 caagtagcgg gttatcgcca attcctatag tcgtgtagat tccggtctgc tcaaacacat
     9541 ctttatggat cagtttagcc atgtcgtacg catttgtttt accaaagagt gccatgctat
     9601 ttgttacatc aaggaagctt tcatcgacgg aatagacatg atggttcgct tcgtctgcat
     9661 attttttata gatagaattg atttctctgt tcttttccat ataataggcc atccttgggg
     9721 caacaatata taaatcactt ggatagggga agggaagatc tctggcacgg ctgatattgg
     9781 tgatgccgta agcttttttt gctgcaggac tggaagccaa gattaatccg ctaccccgtg
     9841 tttctgtcga atcggacgga taggacatga ctactaattt agttgtaagg ggatcaagat
     9901 ccttttcaac gcactctacg gaggcgtaaa aagatttgca gtcaatacat aaaatatctc
     9961 ttgaaggttc tttttcataa acaaataccg gattttgatt cgcccacata acaatcacct
    10021 cttcaacaca attagaacac acgttctctt tccgtgtcaa taaaaaacag caattttcaa
    10081 atacgagcaa aaggctcaca tttggaaatt gctgagaggt tatttttatc tatttgtttt
    10141 tagctgagat cgttgatttt tttatcaaac atgaacagct tctggatcga tctttgttgt
    10201 cccatttcca atgtgtgcaa cataaaattc cggagcatag ccgacaactt tcatgtattc
    10261 atcatagaca ttgtttttga aggcaggtat attttcttct ttgacaagag caatcgcaca
    10321 gccaccaaat ccagctcctg tcattcgtgc acctaagaca ccttcttgtt tttgagcagc
    10381 atctactagt gtatctaatt cgatcccagt tacttcataa tcatagcgaa gagagtggtg
    10441 agaatcattt aacagttgac caaatgcttc taagttccca gcttctaatt cagcttttgc
    10501 cttcaaagtc cgctgatttt cagaaacggc atggcgagct ctgcgtatca atgtatcatc
    10561 gccgatttgg tcaatagttg attcaaattc ttcttcatct aagtctccta aagaagagat
    10621 aggcaaagtt ttttgcaaac gtgccaaggc ttcttcgcat tcgctacggc gttcattgta
    10681 ttttgaatct gccaattccc gccgcttatt cgtattcatg atgacgattg cataaccatc
    10741 tagtttcact ggaaccattt cgtatttcag cgtatttgta tctaataaga ttgcatgatc
    10801 tagctcaccg aagccgatgg caaactgatc catgatacct gaattgacac cgatgaattc
    10861 attttctact tttttacctg tttggactag ttccaagcga tctgttttta aatcaaacag
    10921 atcttctaat acaacaccta ctaataattc taatgaagcg ctactggaaa gtcctgcacc
    10981 attagggatt gttccttcca ctaataactc aaacccatga tcgattgagt gtccagcttc
    11041 ttttaattta aggaccattc cttttacgta atttgcccag tttgcttttt tatcatattc
    11101 tagctcatct aaagtaaaac taatgacacc gatatcgtca aagtttgtgg aataaactaa
    11161 tattttatta tcgtctcgag gtgctgctac accttgcgtt ccataagtga tggaagcggg
    11221 gaagacatgg cctccattgt aatctgtgtg ttcaccaatc aaattgatgc gtcctggtga
    11281 gaagtattgt gtagtttttt taggtccgaa tgtttcggca aattttgtgt ttaagcttct
    11341 tttgatttcc atgatgagtt attcctttct tgttttgatt ttgaattctg tcactgtttc
    11401 aaatactgat ccggctttta aatgaatcga accaaatgct gagaagcgct ctgcacctgg
    11461 agctgtttga gtctcaaatg tgatgccacc gtgatgtgcc agttttcggt tgcgcatttc
    11521 tggtgtttct gtgccgaaat ttgcagtgaa aataaccaca ctagaagcat ctgtagcaat
    11581 atctacttgg attcttttat cggggctagt caagcgagcg gcttctttac ccaagccggt
    11641 ttcttttaag aaaaatggat gatcgattcc gtcaacaaga tttttttgat ccaaatcact
    11701 agcaaaaacg gacgctaatt ttttaggtat ttgaaaatcg aaagcacttc ctgcagcatt
    11761 ttcttttacc cctataggaa tactatctgt tcgcaaagga gcataagctt cgctattgag
    11821 ccaaagctca tgttgatcga ttgactgaga agcatctccc gtcaagttga agtaaacatg
    11881 atttgtcgga ttgaataaag tatcttggtc actggtgccg cgagaggtca ctcgccagat
    11941 attatctttt gtcaaagtat agcggatttc cacatccatt gtacctggaa aaccatgttc
    12001 tccgtcggga cttgtagtag taaaaatgac agaagcttca ttttcgccat tcaaaatgac
    12061 ataagaccat ttttttaatt caaatccagg agcaccacca tgcagattat gtccagtttt
    12121 tggatctgtg gctaattggt aagtttttcc attgagagaa aagtggccat tttcgattct
    12181 ccctgcggtg cgaccgatag aagcaccgat atacggatct ttttcgatat attcttcagc
    12241 ggtgtcaaat cccaaaacta attctcgatc atttgatttt aaactgacga ttctagcacc
    12301 taaatcagag atagaaagtg ctagcttgtt tttgttctcc aaggttatca agtgataacc
    12361 ttggccaaaa tctttttctg taatcttcat tttcaagatc ctttcttaat gggaaggatc
    12421 tactgcatcg tgaagttcat cttccattgt atcagctgga gccaatttgt tttccaattt
    12481 ctcaacgatt tgtgcatgtt ttttctctgt caatgtaatc ttccaagcaa aaataatagc
    12541 ggagaaaatc atcaaagcag caggagcata gaatgcataa gctttgaaag tcgttacgcc
    12601 atgagcagtg atatcagcag cagtagcatt accagtcatc ccagaagcga tagcgacaaa
    12661 ccctacgatc ccgttagaca aagcaccagc gattttatct agtaaagggc gaattgatag
    12721 agtaacagct tcgttacgga taccattttt ccattgacca tattcaacag agtctgtgat
    12781 cgtcatcaaa gcggataaga aaatcaattg ttgagggaag tagaacaata ctagcccgat
    12841 gataacgatt ggcaaacttg aacctgcgat cgtaaagaaa aagtaagaag aaatcatcat
    12901 tgcgatccct ccaaggtaaa cgtagcgacg agagatgacg cgtgtaagca ttgggaatag
    12961 tggaacagct actaaaccaa gtaaggcagc aactagacca acgatccaaa attcagttgg
    13021 ttggccaagt acatatttga agaagtagaa taatacggca gttgttgctg cattggcaat
    13081 cgcataagcg atataagcaa gggcaagcca catcaactga tcattttgag caactgcttt
    13141 gaacacatct ttgatcccag ctttttcatt ttgctgacgg atcgcagtat cgacttcttt
    13201 tgtgcccaag gcagtgataa tcgcagtacc gcctgaaata acagcagcaa tcacaccgaa
    13261 ccagaaccag cctgaagcgc cttcaatatg tttatgagta acaagatagg taaagaaagt
    13321 aacgataggg acaacgacta gagtagtacc atttgcacct agagaagagc cgaaacgagc
    13381 aaacgtagca aatttttcac gttcctttgt ttccgtactc aaagctggaa tcattgacca
    13441 aaaagcgata tccttaaatg aataaaaact atccaataag atgaaaacga ttgcaaataa
    13501 tatgataaat aaagtcgtat tatgagtagc taagccaaag aaattggtaa agattgctac
    13561 caagagtacg gaactaacaa cgccgccgat aactaaccat ggtttgaatt taccgtattt
    13621 tgtacgagta ttatcgatga tgccaccaat caaaggatca aaaataattt caactaagcg
    13681 gatacctaca actaagcttg taactgtagc aatcattttt gcattcgttg ccttatctgt
    13741 tcctgcgaac atctggcttg tcacgaatac cataaaataa gtgcttaatg taacatagaa
    13801 tgtgtcgtgt ccaaaagcgc ctaaagcgta ggacacacgt tggatcattt tgcttttcat
    13861 aatctgtatt cctctcctgt tcaatatata atactaagta aaccggttac aacgaataac
    13921 aaagatcttt taatgactta aacttaacaa gaaatcgctt cctttttaat atatttctgt
    13981 gacttttatt taactttttg ttgcattata ctaaaggagt aaggagggat agacgtggag
    14041 caatcgattt ttttaaaaaa ggaacatcaa caagtagaaa aagaattgta ttttgatttt
    14101 tgtggtttct ctaaaacgat gccgtttcat tcttttggtc ctgctattcg caatgattac
    14161 atcctgcatg tcgtgatgga aggcaaagga tcttatcatg tgaaaaatca acaattccaa
    14221 ttgaaaaaag gtgatctgtt cttgattcgt ccaggagact cgacgtttta cttatcagat
    14281 ggagaagatc cgtggatgta ttgctggctt tcatttggtg ggagtgctgc agaagaaatc
    14341 attcggcagt cgcttttcag agaagaccaa tacacgatgg tctcttctga catacaaaaa
    14401 tatatcgaca ttattttaga atgcatgaaa gatacggaag aaaccttgga aaatgaatta
    14461 cagttaacag cattaaccta ccgtttttta tcagtccttt taaaagatgg cggaaaggtc
    14521 agcggtggtg tacaaaaggt tcattcctct ttagctgttg aaacagtcaa atacatcgag
    14581 gcacattatg ctgaaaaatt gtctgttgag gaaatcgccc agcatttagc ggtcaatcgg
    14641 agccatttgt cgcgagtatt caaaaatcat atgggtacca gtatcaagga atatttgatc
    14701 ggggtacgga tcaatcgagg ggcgtttctt ctttctctga cggatgaatc cgtggaaagt
    14761 attgcctatc aagtagggtt caatagtcta gtcgtattta gccgaatgtt caaaaagagt
    14821 accggagaaa cggctacgaa ctatcgtaaa cgtatgaaaa acaaaaattt taagaatatt
    14881 gctttggata ccttgaaaaa acagttggaa aaacaaacca cagtttcttg gacaacctga
    14941 ttagtctggt taaaatgaca agcatcggca aaagaaactt gtggcggtta tgctacactg
    15001 taaagcgaag actaaaaagg aggaaaaggg atgcgtacac ttatcatcat tagccatcca
    15061 acgatcgaag aatctttaaa tcagcaattt ttcaaagaag cttctgctgg tttaccagct
    15121 acttggcatc atttggaatc aagttatcca gacggaaaaa tagatataga gactgaacta
    15181 cagctcttgg cccagcatga tcggatcatt ttccagtttc cgttttactg gtatagttct
    15241 cctgctcatt taaaaatttg gcaagataat gtcttggaac gagcagcaca tgtattaaaa
    15301 gggaaagaac taggaatcgt attgacaaca ggtgtggcgg aaaaagaata ccaagctgga
    15361 ggaaaagaag aatatacgat ttccgaattt ttgaggccgt atcagcgaat cgcaaataaa
    15421 ttccatatga cctatcttcc gccattcgtt ttagcacaat ttatgtattt atcccaggaa
    15481 aaacgttggg agaagctaat tgcttatcag cagtatctct cattggaagg caaaccatcg
    15541 ctgactcagc ggattgactg gtttattcaa agagtacagg aaaatcagaa aatgcaggaa
    15601 gaagaatctg aaaaacaagc atatatcgta gaagcactga ctgaggcaaa agagcagatc
    15661 gaagacttga cctttacact gcaagaaatg aaaggaactt cattatgaca aatgattggc
    15721 tgatccaaca attagatgaa ttaagcgaaa aatcagaata tcaagaccgt gcgctcttat
    15781 atgaaataaa gaaactgatg gctgagatag acgaacgaac aatgcagctg caaggagaaa
    15841 tcgacggaca atcttggaat catctcaact ggtgaaattt tcttataaat aacaaagata
    15901 aaaatgtgca cagcgacaat tatttgctac aataagaata tgaagcgttt gataacgcta
    15961 ttatacataa aaattggtgg tgaaaaaaat gtatgatgaa aatgagtggg gagaaaatgt
    16021 tcggatgacc cgaagaagac agcaaaaaca ggcagctcgt aagcggatga tcatgattgg
    16081 ggcagcagtt gccgtgtttt tgatcatagc aggaggagtt gcgggtctat attttcatgg
    16141 aaaaaattcg tcagaagcga aaagtacgac cataacggaa acaacgacaa agaaaacaga
    16201 gacgaaacaa acaagtgagt catcaactaa aacaaaagag acacaaactt ctgaagaaaa
    16261 aacagcaaat ggtttgccag agcaacttca agcaatcgta aatgattatt cgaaaaagat
    16321 cgaagaaaag acgcctgcat tgattgaaga ataccagaca gaaattcagg gaaatcaaga
    16381 gggaatagct ggtttatcag cagttgctaa ccaaaaagca cgtgagcttc aagcaatctc
    16441 cgacgaaggg atcagaaaat tacgtgcagc gtatcaggca gcagaaaata aagacggtgt
    16501 agacttagat actttgatca atcaattatc ggctaattat acgaatcatg tcgctaagat
    16561 ttccgatatt tatttgcaga ctagcgcctc tcttcaagca gagtcaacaa gcacacaaga
    16621 cacaacatct tctgattctg agacagcaga gtcggtagaa gcttctgaaa tgcaagctcg
    16681 aacaacacaa gacacaacag attcctctga aactaatacc aacagcgcga cttcacaggc
    16741 tgcgacaacc gtagttttgg aaggagaagg tccaaatcaa atagccgaac gaactggcgt
    16801 accagtagaa acgatcttgt cgctgaatgg gatgagcatg gagaattatt tcctgactcc
    16861 cggtgagacg ctaaaattga attgataaaa gcaataaaaa agaacgaagc ttccactaag
    16921 gtgaagcttc gttctttttt tattttccgg ttcttttttc gcgtacaaag taatggatcg
    16981 tcatgtaaag caagaaaacg agtccaataa tcaatgagat tctcgtttca ggattgataa
    17041 acatgaaaat caatacgata gctaaaaaag caagtgtcac ataattcgta taaggagata
    17101 gtggcatttt aaaaggatga tcatgcattt tatctgcgtg cttctttttg aagcgaatct
    17161 cgctgatcaa aatcactacc caaggaacca taccaggcaa aatacttgag ctgtatacga
    17221 aaacgaaaat attttctgac tggttgatga acattggtag aagataattt aagatcagac
    17281 caattaagat tccacctgag attgctacga caggataaaa aggcgcaccg tgacgagata
    17341 gtttcagaaa ttttttagac atctttccat ttatggcaag tgtatagacc atacgacttg
    17401 cgctgaaaat ccctgaattg caacctgata atgccgctgt aatcacgaca aaattaatca
    17461 gtcctgcagc aaatgtaatg ccgactttag aaaaagtttg tacaaaaggt gaaccaatct
    17521 cactcaattg attccacgga tagatactca caatgataaa aatagcacca atataaaaaa
    17581 tcaagattcg accgatagtg gactggacag cttcaatgat cgtcttttta ggattctctg
    17641 cttcaccagc agtcattctg atcagttcga ttccttgata ggaagcaaca acgatcgata
    17701 atgcaaagaa aaatcctttg acgcctccag tgaagaatcc accatttttc caaagatttg
    17761 aaatccctac tgcctgacca tgattaccaa atccaaataa gattacccca agacctgcaa
    17821 tgatcatcaa aatgatcgtt atgactttga tgatcgaaaa ccaaaattct aattcgccaa
    17881 acgccttgac agaaataaga tttgccagca taagaaacaa gacgacaatg attccgggaa
    17941 taattgccgg caaatcaggc caccaataat tcatataaga tcctacagca atcacttcac
    18001 ttattcctac gacaatatat tgaaaaatat tactccaagc ggtcaagtag ccagccacag
    18061 gatggatgta ctcgctcgcg taatttgcaa aagaaccagt cgaaggatca acgtaaagca
    18121 tttcgcctaa agcacgcata atcaaataca aaaagaatcc tgcaatcgca taggctccca
    18181 tcactgaggg ccctgtccaa cgtatggttg aggaggcacc cataaacaga ccgacaccga
    18241 tcgttccacc tagtgcgatc atttggatat gacgggcact aagacttcga gttaactttt
    18301 cttcttccat acaaaaactc ctttcgttgt tgagtcaaca agaaaaaata atcaaacgtt
    18361 tcaacacatg tttttcatta ttaggttagc cttattgaac tcttgatgtg attctacaga
    18421 aaattttcat aaaatgcgag gaaaattcga aaaaaacttt ttcatcattc tacttgaaaa
    18481 tgctttccat tttagttaaa ctatatttaa ataaaaagtt aggtaaacct aaaaaggagt
    18541 gtaagatttg gaacgtacgg ccattgtaat tcaagacctt tcagccgctt atcaagggaa
    18601 aactgttttg aaaactatca acttgacgat aaatccacaa aaaattaccg gtattatcgg
    18661 accaaacggt gcaggaaaat caacatttat gaaatctttg ctagagttga taccagctac
    18721 tactggtaaa gtcacctttt ctggacagtc ggtaaactca gtccgtaaaa aaatagccta
    18781 cgtggaacag cgaagtgaat tggatctttc ctttccaatt gatgtattag gcgttgtact
    18841 tttaggaaca tatccatctt tacgaattgg acaaagacct gggaaaaaag aaaaagaacg
    18901 tgcaagacaa gctttgaaaa aagtagggat ggaagaatat gccaaaagac agatcagcga
    18961 actatcgggt ggacagcttc agagagtttt tattgcaaga gctctagccc aaggagcaga
    19021 atggatcttt ttagatgaac cattcgtagg gattgatgcg ttaagtgaac gaaagatctt
    19081 tgacatcttg caggaattga agaattcagg aaaaacgatt ttgatcgtcc atcatgatct
    19141 tcataaagta gaagaatatt tcgatgaggt tattctttta aataaacagc tgatcgcttc
    19201 cggtccagta caagaagtct ttacatcgaa gaaccttcaa ttggcctatg gtgagatcat
    19261 cagacattta gtaaaaggag gagagaaaaa atgatccaat cctttattga tggattaatg
    19321 aattatcaat tcctgcaata cgctttggtc acttccatgc ttgtaggact tgcttctgga
    19381 gtcatcggtt cttttattat tttacgaggg atgtctttga tgggagacgc tatctctcac
    19441 gcagttcttc caggtgtagc catttcttat atgtttggtt ttagttacat ctttggagcc
    19501 actgcttttg ggatgctaac agcagcagtg attggtttcg tgactcagcg gagccgattg
    19561 aaaaatgata cagcgattgg aattgtcttt agctcctttt ttgcattagg gattatcttg
    19621 atttcatatg cccagagtgc gacagatttg tatcatattt tatttgggaa tgtcttagct
    19681 gttagggaaa gcgatttgtt gctgacagca ctcgttagtg gaatcgtctt gatatttgtc
    19741 tttttctttt ataaagaatt gaagatcaca tcattcgatc cgacaatggc aaaagcttat
    19801 gggctgaaca catcgctgat ccattatctt ttgatgttct ttttgacatt agtggctgta
    19861 gtcagtttac agacagtagg aacaatcttg gtgattgcca tgttgatcac accagccgcc
    19921 acggcttact tgctaacgaa ccatttactg aaaatgatca ttacagctgc aggaatcggt
    19981 atgctaagtg cagttgtcgg tgtgtttttc agttatagtt acaattggcc atcaggagct
    20041 acgatcgtgt tagcatgtac cgcatttttt atccttgctt ttttattttc tccaacaaaa
    20101 ggaattttat ttcagaaagg aagcagatag atgaaaacaa aaaaatcact ctttttaatc
    20161 ttggcagtta gtttcttggt attagccggt tgcggaaagc aggcttctga ccaagcggat
    20221 gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt cgatcttggc ggacatggca
    20281 aaagaggtcg gaacagatca aatagatatc cacagtatcg taccagtcgg aacagatccg
    20341 catgaatatg aagtactacc agaagacatc aaaaaggcaa gcgatgcaga tgttatttta
    20401 tacaacggtt tgaatcttga aacaggtaac agctggttcg ataacttgat ggaaacggct
    20461 aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa atgtagaacc tctttattta
    20521 actagcggtg aagaacatac aaaagcagat ccccatgctt ggctagacct atctaacgga
    20581 ataaaatatg tggaggaaat cgcacgtata ttctctgaaa aagatgcaga aaatgcgaca
    20641 ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa aagaattaga tacacaagcg
    20701 aaggaaagtt ttgcttctat cgaagagaac aaaaaattat tagtaacaag tgaaggggca
    20761 ttcaagtatt tttcacgagc atatgatttg ccagcagctt atatatggga gatcaataca
    20821 gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg atcagataag agcaaaagaa
    20881 gtaccggttt tattcgtgga aaccagtgta gactcaagaa gcatggaacg ggtagcaaaa
    20941 gaaacgggtt tgaaaatcta tgataaactg ttcactgatt ccatagcaaa agaaggagaa
    21001 caaggagatt cttactatca gatgatgaaa tggaatattg aaacgatcca tgaaggtttg
    21061 tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt gaggagcaga ccttgcattt
    21121 tttataagag atatgaaaaa aaattatgct gtttttcgtc gtacagatac cttttttgaa
    21181 gaaattccgt tatagttaga gtaaggaagg aaggtgtggc tatgaatttt acacgtaaag
    21241 ataaatatac gattaaagaa gaagtctttc gattgatcag ccagcaggaa aataccgaca
    21301 aggatcaaga ccttcaattt cttgaagcag aattagctaa attattcata gcaaatcaaa
    21361 atgagacaac agaaccagat gaagagctgt acataggcaa atggttgttt gatgaatttc
    21421 agcctttaga tgaaaatacc ggtatcggtt attttgtgtt cctttctcca gtcggcttca
    21481 cacctggcgt attagttaaa caaaacgacg aaataaccgt caaagcatgg cctgcattat
    21541 tttcagtcat caaagcagaa ggagtaatca aaaagatcca gtcggcctat tataaaagtc
    21601 aatttattgc caacagcaaa ttaatgaata tgttttataa tcgtctagta gaaattgcta
    21661 ggcctgggaa aatcccgcaa acattcacgt acacacagca aaaaaatctc tatgaagaag
    21721 tattgtattc ccgcgatctg tctgagtttt cgccatggga agaggtaagg ctccatgaac
    21781 tgttaagtac aaaaccagtt tcattgaatt tttttaatgc attagataac aaaagaaaat
    21841 taatttcaac aaattattgg gaagtatttg ctgcaagaaa aaaatggatg gaagaaaaga
    21901 agaatgccga tcagcagatc cagtttgtcc agctgaaaaa agaaaaacta tattatgggt
    21961 tatacagccg ttaacagcaa gcgaggaaga ccatgacatt aaaagaagaa atcatacaag
    22021 aaagcaaacg attaggaatt gacaaaatcg ggttcaccac ggcggaacct tttgattcat
    22081 taaaagaatc gttggaagaa caaaaagcag ctggccatac ttctggcttt gaacatccaa
    22141 atatcgatga gcgtctttat ccagaaatga catttgatca gccgaaatcg atcatttcca
    22201 ttgcacttgc gtatcctact aggatccatg aagaagtgcc tagggatgaa aaacgcgggc
    22261 agtttgctcg tgcttcttgg gggatcgatt accatgatat actcagagac cgtttgaacc
    22321 gattgatcga attcatccaa acaagagcag aaaaatggca aaaagaagag gaatggcgtc
    22381 tagctccgca agtagatact ggagaactga tcgatgtcgc tgtagcacag cgagctggtt
    22441 taggatttat tggacgaaac ggattgttga tcactgaaga gttcggttca tttgtctatc
    22501 ttggtgagat cgttactaat attgtatttg agcctgataa accaggagaa tttggttgcg
    22561 gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc attgcttggt gacggaagaa
    22621 tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa agggatgatg ccggaagaat
    22681 atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat ctgccagtta gtctgccctt
    22741 acaatcaagg aaaagatttt cattttcatg aagagatgga accaaaagta gacgaaatat
    22801 atccaaaatt aaagccaatg ctttcgatgt ctaataaaga cttcaaacag caatttgggc
    22861 atttagctgg ttcatggcgt gggaagaaac ctttgcagag aaacgcattg attgctttag
    22921 ctaatctagg tgatcgaacg gcattgcctg agatttcaga atgtttaaca gatgtccgcc
    22981 ctgtcatcag aggaacagca gcttgggcta ttggacgtct aggaacaaaa gaaccggaaa
    23041 aatggcttga tgtattagat gaaatattag ctaaagaaac agaagaagtc gttataaacg
    23101 aattggaaca tgcccagaaa ttattgcgga gaaagttaaa ataaaaaaat agttaacatt
    23161 attcaaagtt tttgtccagt aattgttgta acaattactg gtttttttgt attttttaca
    23221 tttttttaag agaatttgat aaaaatcaaa aatttttttg ctttttttta tggattattg
    23281 cagtgaatgt tacagaatct gcgtttttca ttcaaaaata aaaaaaacat agaaaaacga
    23341 ttactttgct tatacaacaa ggcttttatg gttttgtatc aaaaaaatat ttgtttaaat
    23401 gttacaaata ttaaataatt cgttattttg tcattaaatg tcatgtttct tttacctatg
    23461 aaatatttct aaatgataaa gttaatagcg tcgaaagaat aaaaaacgga gaaaaaaagg
    23521 agtttaatat gaattcattt aagaaaattg ttttaggtac aacttttgct gctggtgcta
    23581 ctgcaatgtt tgtaggaact acaaatgctc acgctgatga agtttataca gtaaaatcag
    23641 gtgattcatt atctaagatc tcacaaaaat ttgcaggaga caactcaatg atcgatgcaa
    23701 ttgctgaaaa aaattcgatc gcaaacatca accgcattta tgttggtgaa caattaacaa
    23761 tcccaacttc aaatgattct tcagcaacaa cagaaaacaa aacaacagaa aatacagcat
    23821 ctacaactga aacagcaacg caagaacata catacgttgc acctgttgaa acagttgaag
    23881 tagctcctgc tgccccagct gctgcaacag cgccaacttc aagctctgca aaagaatgga
    23941 tcgcacaaaa agaatcaggc ggttcataca cagctactaa cggacgttac atcggccgtt
    24001 atcaattaga tgcttcatac ttaaacggcg actattcagc tgccaatcaa gaacgtgtag
    24061 cagaacaata tgtaacatct cgttatggtt catgggatgc tgctaaaaca ttctggttag
    24121 caaacggctg gtactaagcc gataaaagga aaatgatgaa accgagaaac ttgtttattc
    24181 aagttctcgg tttttttgtg tgaaaaataa ctcgtttgct tcccacagcg cagcagtaca
    24241 aataaaaact cgaataatgt ataataaaga aaacgttttt ttgtttaggg aatgaggagg
    24301 agccatgagt acaaaagaga gtctcgaatt aaacgaaatc acaagtggaa atccactgtt
    24361 atccagtatt cgatcgattg tagaaacgac attttacggt aataatgttc atgaagtttt
    24421 tgataggaag acagcttatc aactagcaaa agggagtcca ggaacgatca tcacagattt
    24481 aacaatctca catgctgaag aactagattt accagctgat gttaggacac tagtattcaa
    24541 tgatggaagc attgtgggaa gaacagcaag tgctcggcgg atctttgagg acttagacaa
    24601 ggaacaaagc aaatacgaga aaattttaag agaagctgtg tatcaaagtc gcaagcgcca
    24661 gttttaccat acaaaagtaa tcgttggcct atctgaagaa ttttctgtgc aaagtcattt
    24721 gctgattcca gaaggatttg agaacaattt gtattcttat ttgctgaatt ttcagccagc
    24781 gaattcgaag atcttagaga cttatcaaaa gtctgtttcc tttgatgagg gagatcttta
    24841 tatcttttgt gatcccgaat ggacacatga aacatttcca aatggactca ttttgtttga
    24901 tgctctccat aatacagcgg ttgtgctagg attacgttac tttggagaat taaaaaaagc
    24961 gactttgacg cttgcttggg cattgggaaa tcgcaatggc tttattgcct gccacggtgg
    25021 gatgaaacaa tatgctagta atgaccagac ctatacgatg gctgtatttg ggttgtccgg
    25081 ttctggaaaa tcaacgatta ccttagcaaa acatacggaa ggtcactctg tttcgatctt
    25141 gcatgacgat gctttcgtta tttctcgaaa aaatggtttt gctactgcct tggaaccttc
    25201 ttactttgat aaaacacaag actatccgtc tgatgatccg gccatttctt ctttccttac
    25261 ttgtcagaat gtgggcgtga catgtacaag taaaggagaa aaagtcctag taacaggaga
    25321 tatacgaaac ggaaatgggc ggacagtcaa atcacgttat gcgacacccg accgagtcga
    25381 tcatcttagc gaaaagatcg atgctgtttt ttggatcatg aaagatgatt cgcttccgcc
    25441 aattatcaag atcaatgacc cagttttagc agcgtctttt ggtgcaacat tagcaacaaa
    25501 acgatcagac gcggaaaata tcactacaaa aagacagcta gatgagctag tcattgaacc
    25561 atttgctaat cctttccgtg tctatcctct tagggaagac tatcaagctt ttcgagagtt
    25621 gtttttgaaa cagcaaacag cctgctatgt cctgaatact ggatatttcg atgataaaaa
    25681 aatcaaacct gaaacgacat tgtcaatcgt tgaaagaatc gtgcaacaaa cggcagaatt
    25741 caagtcgttt ggtccatttg aagacatcca ctatttacct ataccagatt ttcctgtttc
    25801 ttttgaggat acgtcctata aaaatctggt gaaacaacga cttgatattc gtttacgatt
    25861 cattgaagct cagataggaa aagatctctt gccagatgaa gcaaaagaaa gtttggagaa
    25921 tttgattcag aaattgaact aaaataacag ctttttcaag aagtaatcca tgcgcaatat
    25981 cgctcgggat ccttacttga aaaagctgtt tttctatgat ggatttttcg taatataggt
    26041 ggagatgtcc ttttgataat cttccgtagg ccaatgacca gtatttaaga agttggctaa
    26101 tagatagccg atataaggac ctgtggtcaa gccagaagag cctagaccgc tagcagccag
    26161 taagcttgga tcatctggca gtacgccgaa aaatggggaa aaatctgacg tataagcacg
    26221 ggtacctaca cggaactcgt atggccagtt ttttatctga tctggatcag tcaagaactg
    26281 ttctgtactc ttagtcaatc ccttataagc ttcgctagta ggagccaaat cccagccttg
    26341 atcgttttca tgggtagcac ctaaaaggat ctttccatcc atgaaaggaa tgaaatcagc
    26401 ttctccgtca agcatagcaa caggccagtc ttgactcatc ttataagatg tctgaaatgc
    26461 taagagctgc cctttttgcg gacgtatatc tgcagcatag cctaattctt gtaataaagg
    26521 cttaagtgcc gggccaggtg tcaaaattac acgatcagct gtgtattctc caaacggtcc
    26581 cttgatgagc caagaatcgc tcttcttatg caaagtgact tttgcctgga tctgctccat
    26641 ttgtttttgt gctaaacgct gaccaagatg ttctaaatat gcttttccat ccagacgacc
    26701 accaccactg atacgcagtg aaactgctgg tttcaataac ggaagtgctt gagatgtctg
    26761 ttcaggtgaa agccattcga tgtttccaat ttctggcgct tcttcttttc gtttctcggc
    26821 aagaacagcc aattcagcta aatctttttc aggtcgaagc aaaagtgtcc ctgattgtgc
    26881 gtaaatcgtt tgatcaagtt ggaaatcttt tataagttta gggaaaaaag cagcgccgtc
    26941 tttagccaga cgataccatt gtttgtttct gcgtttcgac aaccaagggg agataatccc
    27001 agcgctagct ttagttgcct gtcctgtccc ttcatcaaat aaagcaacat tgaattttga
    27061 tgtatctaag taactggcta gggtcatacc aatgatcccg ccaccgataa tggcaatttt
    27121 ttccataaaa aagcctcctt ttacaaacaa ccattttcac acaaaaaaac ggcagatgca
    27181 acacctgccg tatatggaat ctacgctttt ttagcaaaga acagataata ggcgaatcga
    27241 gcaaagctta cgagaagcaa cagcaacaag acgcctaatg ctgcttttga tcctaatgta
    27301 gtagacactt catagttatg ggaagtatga tgctggacgt aattgataat gaccgctact
    27361 aatgaagtag ccacagcgcc agaaaactgc tgcaaggtat tgaacattgc gttcccgtct
    27421 ccttgtagtt cttcattcag tgcattcatt ccggtggtca tcatattgct gtaagataat
    27481 ccgataccga tcatataaaa cacatgacaa gcaatcaata cacccactga agcgtgtcct
    27541 atgagtagga ataatgctag ccaaccaatc aaagaaagaa tcagcccagc aaaaattggc
    27601 ttcttcggac catattgatc cagcattttc ccggaaaaag gagaaagcaa agcaccaacc
    27661 gctgcgccgg gaagcatcgc taatccagcg acaaaaggac tacttccttg aacgatctga
    27721 aggaaattcg gcaagacaaa agatacacca agtaataaaa actgataaac taagaagcca
    27781 atcaaaaaca agcggaatgt tttatttgaa agaatagaca gtcggatcag tggatgctcc
    27841 acttttttac ttcgatagta aaaaaggaac cagccaatac cgccgatgat taaagcaatc
    27901 cagctggaaa ctgttcccaa cgtactcaaa aatgttagac ttccactaaa caaaagacaa
    27961 attcctaaaa cgcttatcca atccaaataa ccactttttt gtactgccat ctcgggaata
    28021 gcaaaccagc cgataatcaa agaaagaatc aaaacgggca caagaaacag gaagatatga
    28081 ttccatgtaa aatgtgaagt cataatgcct ccataagtgg ggccaattgc tggagcaatg
    28141 gccgttgtca aagtaccgac acccatcatc gcgcctcgtc gattgagggg ggcaaatgtc
    28201 aagataatat ggaacatcaa cggaagagcg atacctgtcg ctgcaccttg gagcaatcga
    28261 ccaaacaata ggaccatgaa ggtaggagag aagaaatcga tcattatacc tgccaagaaa
    28321 agcagatttg aagctaaaaa gagttttttg agactaaaat tgcgattcaa ataagtagac
    28381 agcggaacaa tgatcgaaat catcaaaaga taaatcgttg ttacccattg gacttgtgca
    28441 gtcgttaaat caaactgtct gataagagtt gggaaagtta cattcatggc cgtctcgatc
    28501 aatacgcccg caaaagacat catccctgtt gcaattactg caggtactaa aagcagctgt
    28561 ttttgttttg tcatttacat cacctctaaa aaatttcgta aaaaaagaga gtgatttcta
    28621 ttataaaaat agaaaccgcc tctctctcga cacgccgaaa cgtgttatct gtacgattta
    28681 tttacttcac ttattctagc acaaactgaa aaatagtgtc aacacgatta gccaagtcac
    28741 tcaaaaagag gtatgataat aaataactaa atggaatttc gcggaggaag aaaaatggaa
    28801 aaaattgaat ggcgtaaaga caagagctat agtatgaaaa aaagcccgca aatactagag
    28861 gttcctaaaa aatcattctt tagcatcaga gggatcggcg acccgaatca ggaagacttt
    28921 aaacgaagag tagcctgtct atatgcagtc agctatgcaa tacgcatgtc gcctaaaaaa
    28981 aattggctga ttcctgatta tcagatgtat actgtctatc cactggaagg acaatgggga
    29041 ctgcaagaaa agtatttgca tgaaccagtg atgaaaaaag aacatttttc ttatcaattg
    29101 atgatccgac agcctgactt tgttacagaa gcgatagcac aagaagccat tcagcgttct
    29161 agcaaacttc cggaagactt gaaagaacaa gtagtattca aaaagatgga agaaggtctt
    29221 tgtgcccaaa tcttacacgt cgggccctat gccgaagaac cagcaacgtt tgagaaatta
    29281 gaagcattca tcgcaaaaga aggctatgaa agagcttcta aagaacacaa agagatttac
    29341 atcagcgatc caagaaaatc agcacctgaa aaaatgaaga cgattttgcg tgttaaaata
    29401 gaaaaaggtt agcaacctga atgaaaacaa aaaataaaag taaaaaatac caaaatatga
    29461 agaaatatta cttatctttt gctataatca acattgagag gtgtctaata tgaaaaagaa
    29521 acagcgacaa gcactgatcc gccagatcat tacagaacaa cctattggga cacaggaaga
    29581 gctgcttgcg cggctgcatg aagcaggcgc cgatgtgact caagcaacga tttctcgaga
    29641 cattcgagaa atgaagttag taaaaagtca aaatgaaaac aaaatagtac gctatacatt
    29701 atttaaccag ccatctgttt ctttaaatga agaacgtttg cgtacagcaa ttagacgcga
    29761 ggttttgcgt atccagtctg ttcagttcat ggttatcgta ttgactgaac gaaatggtgc
    29821 cgatgtggta acgaattggc tagatgaagt ggcttaccct gaagttgtcg gaacgatggc
    29881 cggtgtagat acattcatta ttatttgccg ctcagaagaa gaagcacagc gatttgcaga
    29941 aaagctggaa aaaatgaggg aataaatgag gaggactttt atatgaaaga tatgaaagcg
    30001 ctgcaaaatg gatcggatat tcgaggaatc gcattaccag ttgaaggata tgaagtaaac
    30061 ttgacgacag acatggtaaa aaagatcggc tggggactag caaactggat aaagaaaaag
    30121 aagaaatcaa ccgatccaaa taaaaaatgg acgattggga ttggtcatga cagtaggata
    30181 agcggaccag cactgaaaga ggcgttgatc caaagtctga aagaacaagg gatacatgtc
    30241 atcgatttcg aattagctac tacgcctgct atgtttatgt ccactcaatt tgaacagttc
    30301 tcgtgcgatg ctgcggttat gctgactgct agccatcttc cctattatta taatggaata
    30361 aaattcttta cctcagaagg aggagcagaa aaagaagata ttgcttttat cctatcacag
    30421 cagcaaaaaa taaaaagcga acaaactgga agcgtagaaa aagcagacct tttgacacct
    30481 tatgcgcaag atttagtcac aaaaattaaa aaggggattg gaacactaga aagcaaacca
    30541 ctaaatggtt ggaaaatcat tgttgatgcc ggaaatggtg ctggtggatt ctttgctgaa
    30601 aaagtattga atagcttagg tgcggattca acaggttccc aatttttgga accagatggc
    30661 cgatttccta atcatatccc aaatcctgat aataaagaag cgatggaaag tatcaagcag
    30721 gctgttttag caaatgaagc agatcttggg atcattttcg atacagatgt ggatcgttct
    30781 gcagttgtca gtgcttctgg aacagtgatc aatcgaaata atttgatcgc agtattaagt
    30841 acaatcgtat taaaagagca tccaggaaca agaatcgtga cgaattcccc tacttcttct
    30901 catctacaaa gattcattga agaaaaagga ggcatccaag ttcgttatat ttccggctat
    30961 cgaaatgtaa tcaataaagc attagaactt aaccgtcttg ggaaagatac acagctagcc
    31021 atcgaaacaa gcggacacgc tgcattcaag gaaaactatt ttctggatga tggtgcatat
    31081 gtcatcgcaa agatcctgat gcttttgcca gaattaaaaa gaaaaggtat cacattagat
    31141 gaacaaatca aagagttgaa acagcccgta gaagtacaag aaatccgatt aaaattgttc
    31201 agcgatgaac cacgcgaaca aggaaacaag atattagaag aaatgaagcg actgttggaa
    31261 gaagaaaaag ggatgaactt cgacccggaa aatgaagaag gtattcgata tgatctagaa
    31321 gagccatacg gaaacggctg gtttttgatc cgtttaagct tacatgagcc gttactagta
    31381 ttacagatag aaaatgatga attcggaaag aacaagttgg cgttagtaaa attagcaaaa
    31441 ttattcggac atttttctga tcttgaatca ttaacagcag aataaaccag tctttttgaa
    31501 aggctagttg cttggacggc aaaaaaagtg gtatggttaa atcgagtgct ggtagggaga
    31561 ccagcaaaag gaggaaaaaa tggtaacgaa tatggttatt tttttaacag tattggttta
    31621 tggggtcaac tttctggtat atatgtatct taaaatcaat catataaaag attatgtaga
    31681 aaagattgct atttattttg gaacaaatat gacgctgttg ttcacgagta gtattttttt
    31741 gttttttgga aaaatagtag aagacggcat tctggtattc gaataagcag aatttttgta
    31801 gaaagcgcct tacttgtcat acactataat tatagccaag tagcaaatgg tcaatcgaaa
    31861 ggagcagtac aatgaaaaaa gcatacgaaa cagcaatgat caaccatggc gggcgcaatg
    31921 gagaagtcga ggcaccaaat ggctcgatgc atatgaaaat cactcctcca ggaatccatg
    31981 cagaaggaac aaatccagaa caattatttg cagcaggtta tgcttcttgt ttcaatgggg
    32041 cggtacaaca tatgatcaaa gaagcaggaa tgacagcaga gtccacagta aaagcacgtg
    32101 tctctcttta taatttagat gatggcggct atcagatcgg tgtagtatta gaagtgcata
    32161 tcgatggcat cagcgaagaa gaagcccaaa aaattgcaga agatgcgcat gaattctgcc
    32221 cttactccaa agcaactaga ggaaatatcg atgtagaagt aacgattgtt tagaagaata
    32281 aaggaaatcg aaccagacta aaaacgtgaa aggaccgctt tttgtctggt tttttttgac
    32341 aaataaagca gatgatttgg agaatagaca attatcggtt atcatatttt tgtaccaatc
    32401 ggtaggtaaa aaagaagcga gggaaaaatg atggaaaaat atcaaaatat tgtgatcggt
    32461 tttggaaagg gcggtaagac tttagcgaag gcgttagctt caaaaggtga atcggtttta
    32521 gtcgttgaaa agtcaacacg tatgtatggc ggaacatgta tcaacatcgg ctgtattcct
    32581 tctaaatcat tgattttcaa tggagaaaga ggaatcgact ttacagaggc tgttgctcgg
    32641 aaagaaaaat tgacgggcat gcttcgcgcc aaaaattatc atatgatcag cgatgaggca
    32701 actgggacag tgatggacgg tacagcacgt tttctttcta accatcaaat cgaagtgacg
    32761 aataatggtg aaaaagtgat tgttgaaggc gaacggattt tcatcaatac tggttctgaa
    32821 ccaatcattt taccaatcaa aggattgaat acaagtcgct acttgattga ttctacgcaa
    32881 gcgatggatc aggaacaatt accggaaaaa ttagtgatca tcggggctgg atatattggc
    32941 ttggaatttg catcaatgtt caatgaatac ggatctaaag ttgtcgttct tgatgcccat
    33001 tctgaatttc ttccacgtga agatgaagat atcgcgcaaa tgattttaga agacatgaca
    33061 aatgcgggaa tcgaattcca tctaggtgta tctgtagatc aagtagcaga ccaagaatca
    33121 acagctgccg tcacctttac agaagatggc caagaagtaa caatccaagc gtcaaaagtt
    33181 ttggctgcaa ctggaagaaa accaagtaca gcatcacttg gattagaaaa tacagatgtt
    33241 aaactaacag atcgcggagc aatcgctgtg gatgaatacc ttcgtacaga tgcagacaac
    33301 atttgggcga ttggagacgt caaaggcgga ttgcagttca cttatatctc tctagatgat
    33361 tatcgaatca ttatggatca gttgaagggc gagaataaac gaacaacgaa taaccggaaa
    33421 gctgtgcctt acagtgtatt tatcactccg actttatcca atgtaggttt aacagaaaaa
    33481 caagccaaag cacaaggcgt caattacaaa ttattcaagt atatgacaag cggtgtccct
    33541 aaagcacaag tattagaaga tcctaaaggt gtattcaaag ttttagtcga ccctgaaact
    33601 gacttgattc taggggcaag tatctatgcg gaagattctc atgaagtcat caacttgatt
    33661 tcgctagcta tgaatggaaa acttccttat acgttattga gagatcaaat ctattctcat
    33721 ccgacaatga gtgaagctct aaatgatgta ttaaaataaa taacgaatgg agaatccacc
    33781 atgttcaata ataaaaatac agcagtagtt ctcactgacc cgcaagtcga gttcttgaaa
    33841 ccagcaggcg gtggctttgg tttgacaaaa gatatcttag aaaaatacaa tacgatcgaa
    33901 aatatgattg aaatggtaaa acaagcaaaa gaaaaaggat acaaactctt tatttctcct
    33961 cattattttt acccacatga ccataaatgg caatttggcg gtgcaggaga acatatgatg
    34021 cttgagcagc agatgtttgc tagaaaaagt caatatgaat cagtggaaga aggttcagga
    34081 gccgatttta tcgaagaatt gaaaccttat ttggatgaaa atacgatcat cagctctcct
    34141 cataaaatct ttggaccaga aagcaatgat ttggcgctcc aattgagaaa aaatggaatc
    34201 gacacagtaa ttcttggtgg aatgaacgga aacttgtgta gcgattccca tatgcgtgaa
    34261 ttggtagaga gtggattcaa tgttgtggta gcgacagatg caattggtgc tcctggccaa
    34321 gaagcaatgg atgcagcgtt gacgaacttt ggctttattg cagatcaagt gatgacaaca
    34381 gaggaagtat tagagcaact atcataagat aggaattgct tttgtcctcg cagttcttga
    34441 atcaatgaac atctagctat atgagaacag ttttatagtg agggctgtgt cagataattt
    34501 ggcacagtct tttttaaacg attaagaaga aaaaatgatg aatttactgt ataatatagg
    34561 actatgagaa atgaaaaagg agaatatcat tgaaaacaaa ggaaaagatc ttggaagcga
    34621 ccaaagatct tatttataaa aaaggctatc atgaaacgtc aatccgagat attctagcag
    34681 cttctgatac aggaaaaggt caattttatt attacttttc ttcaaaaaaa gaagtttgtc
    34741 tagcggtgat ccatgcgcat atggacgtat ggcagacaga attgttcgaa gacatcttaa
    34801 atgctggaaa acagccagaa gaagcattag aagaaatgtt ggactggatt ttcgctgatc
    34861 atgagacaca gatcacttat catggctgtc ctgtaggtaa tttgattatt gagttgagta
    34921 cagtggatga agaataccgt ctgttgctac tagaattagt caatcaatgg gtcaagtata
    34981 cgaaacaaaa tatcctggca ctttctgaag aaaaactgtc cgatgaagaa gcagaccgtc
    35041 aagccaaagc gttgattgct gaaatccagg gaagtatctt attgttgaaa gtcacacagg
    35101 atctatcact acttaggcag aattttcaat tggtcaaaga aagattgtta acttcgcaaa
    35161 aggttcttta ataat
//
LOCUS       DACEKQ010000019        34658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEKQ010000019 DACEKQ010000000
VERSION     DACEKQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.019"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(311..2203)
                     /locus_tag="IW671_000854"
     CDS             complement(311..2203)
                     /locus_tag="IW671_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897860.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2200..3927)
                     /locus_tag="IW671_000855"
     CDS             complement(2200..3927)
                     /locus_tag="IW671_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897861.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3943..4593)
                     /locus_tag="IW671_000856"
     CDS             complement(3943..4593)
                     /locus_tag="IW671_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0897862.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4797..5093
                     /locus_tag="IW671_000857"
     CDS             4797..5093
                     /locus_tag="IW671_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0897863.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5285..5905
                     /locus_tag="IW671_000858"
     CDS             5285..5905
                     /locus_tag="IW671_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0897864.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5997..7163)
                     /locus_tag="IW671_000859"
     CDS             complement(5997..7163)
                     /locus_tag="IW671_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0897865.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7175..7528)
                     /locus_tag="IW671_000860"
     CDS             complement(7175..7528)
                     /locus_tag="IW671_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0897866.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7656..8678
                     /locus_tag="IW671_000861"
     CDS             7656..8678
                     /locus_tag="IW671_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0897867.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9117..9551)
                     /locus_tag="IW671_000862"
     CDS             complement(9117..9551)
                     /locus_tag="IW671_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897868.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9661..11520)
                     /locus_tag="IW671_000863"
     CDS             complement(9661..11520)
                     /locus_tag="IW671_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0897869.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11638..12797)
                     /locus_tag="IW671_000864"
                     /pseudo
     CDS             complement(11638..12797)
                     /locus_tag="IW671_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13104..14528)
                     /gene="araA"
                     /locus_tag="IW671_000865"
     CDS             complement(13104..14528)
                     /gene="araA"
                     /locus_tag="IW671_000865"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0897870.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14544..15242)
                     /locus_tag="IW671_000866"
     CDS             complement(14544..15242)
                     /locus_tag="IW671_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0897871.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15199..16860)
                     /locus_tag="IW671_000867"
     CDS             complement(15199..16860)
                     /locus_tag="IW671_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0897872.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17078..18478
                     /locus_tag="IW671_000868"
     CDS             17078..18478
                     /locus_tag="IW671_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0897873.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18730..19809)
                     /locus_tag="IW671_000869"
     CDS             complement(18730..19809)
                     /locus_tag="IW671_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0897874.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19895..20854)
                     /locus_tag="IW671_000870"
     CDS             complement(19895..20854)
                     /locus_tag="IW671_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0897875.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20866..22389)
                     /locus_tag="IW671_000871"
     CDS             complement(20866..22389)
                     /locus_tag="IW671_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0897876.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22399..22962)
                     /locus_tag="IW671_000872"
     CDS             complement(22399..22962)
                     /locus_tag="IW671_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0897877.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22968..23831)
                     /locus_tag="IW671_000873"
     CDS             complement(22968..23831)
                     /locus_tag="IW671_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0897878.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23845..24741)
                     /locus_tag="IW671_000874"
     CDS             complement(23845..24741)
                     /locus_tag="IW671_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0897879.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24916..26187)
                     /locus_tag="IW671_000875"
     CDS             complement(24916..26187)
                     /locus_tag="IW671_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0897880.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26177..27694)
                     /locus_tag="IW671_000876"
     CDS             complement(26177..27694)
                     /locus_tag="IW671_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0897881.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27892..28782
                     /locus_tag="IW671_000877"
     CDS             27892..28782
                     /locus_tag="IW671_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0897882.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28855..29679)
                     /gene="nadE"
                     /locus_tag="IW671_000878"
     CDS             complement(28855..29679)
                     /gene="nadE"
                     /locus_tag="IW671_000878"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0897883.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29691..31163)
                     /locus_tag="IW671_000879"
     CDS             complement(29691..31163)
                     /locus_tag="IW671_000879"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0897884.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31800..32162)
                     /locus_tag="IW671_000880"
     CDS             complement(31800..32162)
                     /locus_tag="IW671_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0897885.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32385..33137
                     /locus_tag="IW671_000881"
     CDS             32385..33137
                     /locus_tag="IW671_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0897886.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33277..34200)
                     /locus_tag="IW671_000882"
     CDS             complement(33277..34200)
                     /locus_tag="IW671_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0897887.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34215..34487)
                     /locus_tag="IW671_000883"
     CDS             complement(34215..34487)
                     /locus_tag="IW671_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897888.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10228 a         7211 c         5979 g        11240 t
ORIGIN      
        1 agaggttgtg agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt
       61 ctcaggtttg ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa
      121 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata
      181 gcttctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt
      241 tattcggaaa aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat
      301 ttgtactatt ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga
      361 atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca
      421 tcacaataat gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg
      481 tccggttttc caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta
      541 cactagatgt tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa
      601 ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt
      661 tatatccttc tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa
      721 tgatttgttc gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg
      781 taaacagcca agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa
      841 gatcacgggt atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt
      901 caagaaggtt gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct
      961 caccaggttt gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga
     1021 attgaacatt ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt
     1081 tttcattgga catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg
     1141 ttgattggat agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg
     1201 tatattgcaa gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc
     1261 cgcctagtac agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga
     1321 tgattgccga aataaattga gctttccaac cagcggcata cagattctga ttttcttttt
     1381 caaatacttc ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat
     1441 atgtttcttc tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca
     1501 cttgtgattt cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg
     1561 cgactaacgt caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga
     1621 ctgcactagt tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat
     1681 cattgattgc tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta
     1741 cacgattcat tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac
     1801 gtgtcataat aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta
     1861 ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc
     1921 cttgttgcat ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc
     1981 ccaagacctt tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag
     2041 cgatgatcgc aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga
     2101 aatttttagg cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac
     2161 cgcctgctgg tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct
     2221 tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt
     2281 tcctttgcca accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt
     2341 acttactcgt tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg
     2401 gcgaagattg gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat
     2461 atctgctgtt ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa
     2521 gtttcctcct ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc
     2581 tttagcttgt gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc
     2641 atactgcata ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc
     2701 cattaattct cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat
     2761 cgaaccagat tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga
     2821 accagtcccc cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc
     2881 caatgcaagt ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc
     2941 gttttttcca gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc
     3001 atccaacact tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat
     3061 agcggaaagc atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag
     3121 attaccgact tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac
     3181 gtttgtaccg ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac
     3241 tttgattgcc gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg
     3301 tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt
     3361 taaacggtca gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc
     3421 gccgatcaaa ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc
     3481 tttttgataa gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg
     3541 taaaaacatt tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa
     3601 agaggccgtt ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac
     3661 atccgtacgc aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc
     3721 gatagcagct aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat
     3781 ataatcgaca tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa
     3841 atataaatct gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa
     3901 ggagatccgt tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt
     3961 tttcagccaa gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc
     4021 agatgttgaa aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa
     4081 ttggatcagt aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa
     4141 ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg
     4201 gcgactcgta tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt
     4261 acgataaaaa gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga
     4321 ttcgaatcct ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga
     4381 gctgtcacgt ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata
     4441 ctgatagaaa ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc
     4501 ttttaagggt actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc
     4561 tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac
     4621 acattgtcat atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc
     4681 atagtttttt tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt
     4741 gaattaatga gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg
     4801 cccaatcaat tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt
     4861 tagatacaag aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc
     4921 ccgctgctat cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc
     4981 taagctatac aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc
     5041 gtgctgttta tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc
     5101 ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt
     5161 ctttttttag ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc
     5221 gagtaaactg attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa
     5281 gcatatggca tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac
     5341 agttaaggca ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt
     5401 gactgttttg gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg
     5461 ttgggaagca ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa
     5521 aattgctcgt ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc
     5581 aaacgcctta tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa
     5641 tgtagctgga aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa
     5701 aaaagcactg cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca
     5761 atatgtaaaa ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga
     5821 aggaatcgat tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc
     5881 tgctgcttta ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga
     5941 aaaaatcttc aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat
     6001 tctttctgct ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca
     6061 aaaacatgag taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg
     6121 atgattccga acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt
     6181 gctaactgat tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact
     6241 gcaaaaacga tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata
     6301 gccggaatca tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata
     6361 atccccaacg tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt
     6421 gccacagcaa aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt
     6481 tcactcagca cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa
     6541 ggtgctaatt gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga
     6601 gccactaaaa tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta
     6661 gatatatctt ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac
     6721 tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc
     6781 gtatctacat attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg
     6841 atgatcacta cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata
     6901 ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa
     6961 tacaagattc ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt
     7021 gcaaacaaat atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca
     7081 agtaaggctg tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg
     7141 ttcttcttat tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca
     7201 aaccaatagc aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt
     7261 ctggacaagc atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca
     7321 tcggaccgac ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt
     7381 tcaaagaaaa aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag
     7441 ctgttgctgt tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc
     7501 caatttctgt aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca
     7561 gtataacaca ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa
     7621 aactatttca ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa
     7681 cctatacgcg tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag
     7741 aattagccaa cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta
     7801 aagctggcaa tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg
     7861 ataaggattt tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag
     7921 cggttgatga agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg
     7981 tctaccatat attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag
     8041 cgacaggtcc tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg
     8101 accaacgatt agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg
     8161 aaaacggcaa attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc
     8221 atcttgtctt tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag
     8281 tccgcgtgct ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga
     8341 ccttgccaga agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg
     8401 gcaagttcct ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg
     8461 aagatggttc ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg
     8521 atttcgcact agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc
     8581 tgacattata tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct
     8641 atgtaccaga atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga
     8701 atcagcaaga cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga
     8761 aaatcgaaga taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga
     8821 actactttga acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa
     8881 atgaacatca ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac
     8941 cgccgagatg gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag
     9001 gttgttgttg gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag
     9061 gtaatattac atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat
     9121 tttttcaaca acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg
     9181 tcttgttttg cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata
     9241 ctttggatca tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta
     9301 tgaatgccca ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga
     9361 tatttttttg ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt
     9421 tcacttgttt ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct
     9481 tctccgattg cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata
     9541 ttcggtaaca tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt
     9601 tttatcctca aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc
     9661 ttattcgtgc tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata
     9721 ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt
     9781 ataccactgt ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa
     9841 tgataaaagc agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc
     9901 tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt
     9961 actgtcgaac ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat
    10021 cgtatctgaa tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga
    10081 aagattcttc aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg
    10141 cagataattc tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag
    10201 attatcatac tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg
    10261 aggtgttttt tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat
    10321 atatggatta ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc
    10381 aaatcctaat gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat
    10441 cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat
    10501 ttttggtaca agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa
    10561 accagtcaat gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat
    10621 attgccacta tatgtttgat cagctacttc ataataatct gccaatggct caccagatac
    10681 ttctacgccg tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt
    10741 cggcgactct tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt
    10801 gatttctttg atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag
    10861 attgtacaaa aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg
    10921 tatccatagt gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt
    10981 actgatatac cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa
    11041 tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc
    11101 tgcggcagct aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat
    11161 ttcagtgatc atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat
    11221 ataatttgca aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac
    11281 agatcctgct agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa
    11341 gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt
    11401 ttgacaccac tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc
    11461 tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa
    11521 atgcttgctc cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg
    11581 aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta
    11641 tggcctttct ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg
    11701 ttgtttcact tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac
    11761 atgctcattc ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag
    11821 agaaaatatc tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg
    11881 cgcttggaaa aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa
    11941 aagataatct tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt
    12001 cgatccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga
    12061 aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta
    12121 tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat tttttctata
    12181 ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa
    12241 tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg
    12301 cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg
    12361 cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa
    12421 gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata
    12481 cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt
    12541 actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt
    12601 tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta
    12661 agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct
    12721 attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc
    12781 aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat
    12841 tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg
    12901 ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa
    12961 aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga
    13021 ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta
    13081 agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt
    13141 gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca
    13201 cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca
    13261 cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc
    13321 agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc
    13381 ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg
    13441 attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt
    13501 gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct
    13561 cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca
    13621 aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca
    13681 tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga
    13741 ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa
    13801 tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg
    13861 tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa
    13921 ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg
    13981 tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc
    14041 caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt
    14101 tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt
    14161 aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct
    14221 aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag
    14281 aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg
    14341 atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat
    14401 tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt
    14461 gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata
    14521 ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt
    14581 tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc
    14641 tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca
    14701 ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt
    14761 gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat
    14821 tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa
    14881 aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc
    14941 gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa
    15001 acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc
    15061 aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg
    15121 ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg
    15181 agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc
    15241 attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc
    15301 ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat
    15361 ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc
    15421 atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc
    15481 tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc
    15541 tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat tctcagattt
    15601 caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa
    15661 attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac
    15721 tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc
    15781 gccttctagc gctttgttga ataataccgt gaataattta tctctgctta tctccatacc
    15841 caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt
    15901 attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg
    15961 atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga
    16021 aatgttccct gtccctttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc
    16081 ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc
    16141 ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg
    16201 atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc
    16261 ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc
    16321 tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt
    16381 aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca
    16441 tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat
    16501 tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata
    16561 accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat
    16621 ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc
    16681 taatgaatag gtccagatac cattttctag tttgttttcc cactcataac taccagcggc
    16741 aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat
    16801 ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat
    16861 aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta
    16921 caatgattat tgtatacttt tgtacgtaca tgtcaagcag ttatttacaa gaatacaata
    16981 aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc
    17041 aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc
    17101 ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg
    17161 tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc
    17221 tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc
    17281 tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt
    17341 tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc
    17401 agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat
    17461 gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat
    17521 tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagggt tgcctgaaac
    17581 gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg
    17641 tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc
    17701 aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat
    17761 ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga
    17821 aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg
    17881 agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc
    17941 gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct
    18001 ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt
    18061 gatgggactt tctttcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa
    18121 ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc
    18181 tccactgaca tgggtaatcg taggcgaaat attcccacta gctgtccgcg ggcgagcatc
    18241 tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt
    18301 gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt
    18361 aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat
    18421 cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag
    18481 aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca
    18541 caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag
    18601 ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat
    18661 gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa
    18721 acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata
    18781 gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc
    18841 ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct
    18901 taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc
    18961 tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa
    19021 agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt
    19081 ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat
    19141 aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg
    19201 ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa
    19261 acaaatataa gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc
    19321 ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg
    19381 ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa
    19441 ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt
    19501 ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc
    19561 gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt
    19621 gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg
    19681 aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt
    19741 atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata
    19801 ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa
    19861 ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac
    19921 caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag
    19981 gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat
    20041 caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt
    20101 tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat
    20161 tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag
    20221 aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa
    20281 aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg
    20341 tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt
    20401 gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga
    20461 aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta
    20521 tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat
    20581 gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc
    20641 agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat
    20701 gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg
    20761 atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc
    20821 gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat
    20881 tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat
    20941 tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa
    21001 cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc
    21061 aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc
    21121 gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac
    21181 agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat
    21241 cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat
    21301 tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc
    21361 cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg
    21421 actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc
    21481 aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc
    21541 aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc
    21601 atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt
    21661 atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc
    21721 agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc
    21781 cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt
    21841 agcccccact tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa
    21901 aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt
    21961 acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa
    22021 tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt
    22081 tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac
    22141 agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa
    22201 ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc
    22261 atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc
    22321 aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc
    22381 ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag atacaagttt
    22441 ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa
    22501 tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc
    22561 actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca
    22621 ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat
    22681 tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag
    22741 gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc
    22801 tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat
    22861 agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac
    22921 cacaatcaac tgtccataaa gtttagtctc aactattttc atcttcctca ctctttcatt
    22981 ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat
    23041 ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct
    23101 gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat
    23161 ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta
    23221 ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg
    23281 cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata
    23341 ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc
    23401 aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc
    23461 aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc
    23521 gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt
    23581 ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag
    23641 ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa
    23701 ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt
    23761 cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt
    23821 tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac
    23881 attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc
    23941 atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc
    24001 tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga
    24061 accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat
    24121 gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg
    24181 agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac
    24241 agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg
    24301 caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga
    24361 aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca
    24421 aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac caacgataat
    24481 ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg
    24541 ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa
    24601 aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa
    24661 taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca
    24721 atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat
    24781 cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca
    24841 cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct
    24901 gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat
    24961 gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt
    25021 attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt
    25081 cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa
    25141 gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata
    25201 tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg gaatggcata
    25261 tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt
    25321 cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc
    25381 aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg
    25441 accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg
    25501 agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat
    25561 ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact
    25621 actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc
    25681 agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac
    25741 ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt
    25801 aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc
    25861 gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt
    25921 agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc
    25981 aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc
    26041 accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga
    26101 ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga
    26161 gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc
    26221 tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta
    26281 catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat
    26341 caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa
    26401 cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat
    26461 ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg
    26521 ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg
    26581 gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa
    26641 catttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga
    26701 ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag
    26761 gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc
    26821 attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc
    26881 acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag
    26941 aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt
    27001 acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac
    27061 ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct
    27121 ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt
    27181 cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta
    27241 aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat
    27301 ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc
    27361 agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca
    27421 accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa
    27481 aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt
    27541 cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata
    27601 ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac
    27661 gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg
    27721 aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt
    27781 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
    27841 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
    27901 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
    27961 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
    28021 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
    28081 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatatcagt
    28141 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
    28201 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
    28261 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
    28321 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
    28381 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
    28441 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
    28501 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
    28561 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
    28621 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
    28681 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
    28741 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
    28801 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
    28861 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
    28921 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
    28981 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
    29041 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
    29101 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
    29161 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
    29221 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
    29281 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
    29341 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
    29401 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
    29461 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
    29521 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
    29581 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
    29641 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
    29701 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
    29761 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
    29821 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
    29881 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
    29941 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
    30001 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
    30061 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
    30121 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
    30181 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
    30241 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
    30301 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
    30361 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
    30421 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
    30481 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
    30541 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
    30601 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
    30661 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
    30721 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
    30781 tctactaatt ggcagtgcgc taaaggccct tccacttgga ttaatggttc atttgcaaaa
    30841 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
    30901 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
    30961 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
    31021 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
    31081 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
    31141 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
    31201 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
    31261 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
    31321 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
    31381 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
    31441 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
    31501 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
    31561 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
    31621 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
    31681 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
    31741 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
    31801 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
    31861 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
    31921 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
    31981 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
    32041 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
    32101 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
    32161 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
    32221 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
    32281 atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct
    32341 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
    32401 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
    32461 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
    32521 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
    32581 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
    32641 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
    32701 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
    32761 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
    32821 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
    32881 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
    32941 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc
    33001 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
    33061 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
    33121 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
    33181 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
    33241 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
    33301 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
    33361 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
    33421 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
    33481 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
    33541 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
    33601 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
    33661 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
    33721 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
    33781 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
    33841 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
    33901 atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
    33961 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
    34021 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
    34081 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
    34141 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
    34201 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
    34261 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
    34321 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
    34381 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
    34441 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
    34501 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
    34561 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
    34621 ataatgatac tgtaaatata tctttattat tagtgaat
//
LOCUS       DACEKQ010000020        34624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEKQ010000020 DACEKQ010000000
VERSION     DACEKQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.020"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW671_000884"
     CDS             <1..1023
                     /locus_tag="IW671_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0897889.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW671_000885"
     CDS             1157..2500
                     /locus_tag="IW671_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0897890.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..4015
                     /locus_tag="IW671_000886"
     CDS             2612..4015
                     /locus_tag="IW671_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0897891.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4364..4996
                     /locus_tag="IW671_000887"
     CDS             4364..4996
                     /locus_tag="IW671_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0897892.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5584..7023
                     /locus_tag="IW671_000888"
     CDS             5584..7023
                     /locus_tag="IW671_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0897893.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7318..7536
                     /locus_tag="IW671_000889"
                     /pseudo
     CDS             7318..7536
                     /locus_tag="IW671_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7785..8000
                     /locus_tag="IW671_000890"
     CDS             7785..8000
                     /locus_tag="IW671_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897894.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8217..8473)
                     /locus_tag="IW671_000891"
                     /pseudo
     CDS             complement(8217..8473)
                     /locus_tag="IW671_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8660..9571)
                     /locus_tag="IW671_000892"
     CDS             complement(8660..9571)
                     /locus_tag="IW671_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897895.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9590..10594)
                     /locus_tag="IW671_000893"
     CDS             complement(9590..10594)
                     /locus_tag="IW671_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0897896.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10591..12714)
                     /locus_tag="IW671_000894"
     CDS             complement(10591..12714)
                     /locus_tag="IW671_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0897897.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(12719..15166)
                     /locus_tag="IW671_000895"
     CDS             complement(12719..15166)
                     /locus_tag="IW671_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0897898.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15153..15542)
                     /locus_tag="IW671_000896"
     CDS             complement(15153..15542)
                     /locus_tag="IW671_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0897899.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15602..16105)
                     /locus_tag="IW671_000897"
     CDS             complement(15602..16105)
                     /locus_tag="IW671_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0897900.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16118..16342)
                     /locus_tag="IW671_000898"
     CDS             complement(16118..16342)
                     /locus_tag="IW671_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897901.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16446..18356)
                     /gene="ltrA"
                     /locus_tag="IW671_000899"
     CDS             complement(16446..18356)
                     /gene="ltrA"
                     /locus_tag="IW671_000899"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0897902.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19102..19221)
                     /locus_tag="IW671_000900"
     CDS             complement(19102..19221)
                     /locus_tag="IW671_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0897903.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(19236..20420)
                     /locus_tag="IW671_000901"
     CDS             complement(19236..20420)
                     /locus_tag="IW671_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0897904.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(20679..20969)
                     /locus_tag="IW671_000902"
     CDS             complement(20679..20969)
                     /locus_tag="IW671_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897905.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(20959..22101)
                     /gene="dcm"
                     /locus_tag="IW671_000903"
                     /pseudo
     CDS             complement(20959..22101)
                     /gene="dcm"
                     /locus_tag="IW671_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22334..23008)
                     /locus_tag="IW671_000904"
     CDS             complement(22334..23008)
                     /locus_tag="IW671_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0897906.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23086..24426)
                     /locus_tag="IW671_000905"
     CDS             complement(23086..24426)
                     /locus_tag="IW671_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0897907.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24509..24844)
                     /locus_tag="IW671_000906"
     CDS             complement(24509..24844)
                     /locus_tag="IW671_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0897908.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(24854..25228)
                     /locus_tag="IW671_000907"
     CDS             complement(24854..25228)
                     /locus_tag="IW671_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0897909.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25241..25555)
                     /locus_tag="IW671_000908"
     CDS             complement(25241..25555)
                     /locus_tag="IW671_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0897910.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(25668..28895)
                     /gene="fss3"
                     /locus_tag="IW671_000909"
     CDS             complement(25668..28895)
                     /gene="fss3"
                     /locus_tag="IW671_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0897911.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(29309..30475)
                     /locus_tag="IW671_000910"
     CDS             complement(29309..30475)
                     /locus_tag="IW671_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0897912.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            30500..30826
                     /locus_tag="IW671_000911"
     CDS             30500..30826
                     /locus_tag="IW671_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897913.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            30979..32031
                     /locus_tag="IW671_000912"
     CDS             30979..32031
                     /locus_tag="IW671_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0897914.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            32107..32325
                     /locus_tag="IW671_000913"
     CDS             32107..32325
                     /locus_tag="IW671_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897915.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            32312..>34624
                     /locus_tag="IW671_000914"
     CDS             32312..>34624
                     /locus_tag="IW671_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897916.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVREN"
BASE COUNT        10715 a         6628 c         6121 g        11160 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac ttttgacaac ttcatcaagt gaaagtagta cgacttcaaa
     2821 tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc
     2881 aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag
     2941 tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa
     3001 aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga
     3061 atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga
     3121 caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg
     3181 tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag
     3241 tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta
     3301 taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac
     3361 gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa
     3421 attattaact aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa
     3481 tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca
     3541 atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga
     3601 agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat
     3661 aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac
     3721 acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta
     3781 ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca
     3841 ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta
     3901 tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag
     3961 tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag
     4021 gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata
     4081 ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat
     4141 cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat
     4201 gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa
     4261 ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt
     4321 aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg
     4381 tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg
     4441 tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag
     4501 ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt
     4561 tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga
     4621 atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga
     4681 tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg
     4741 attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg
     4801 tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac
     4861 tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat
     4921 agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg
     4981 ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc
     5041 gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca
     5101 acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga
     5161 tatataaaca tctataatat attattcata ctattgatat ttagtataat cctatggaat
     5221 tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata
     5281 aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc
     5341 acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag
     5401 cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt
     5461 acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg
     5521 ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct
     5581 attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt
     5641 gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta
     5701 cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt
     5761 tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg
     5821 aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg
     5881 ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt
     5941 atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt
     6001 cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt
     6061 ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg
     6121 ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc
     6181 catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga
     6241 gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta
     6301 atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg
     6361 atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg
     6421 attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc
     6481 gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc
     6541 ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta
     6601 ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct
     6661 ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc
     6721 cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg
     6781 atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc
     6841 ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg
     6901 ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt
     6961 gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc
     7021 tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat
     7081 ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa
     7141 tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa
     7201 ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg
     7261 agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac
     7321 ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa
     7381 tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc
     7441 cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa
     7501 tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa
     7561 taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt
     7621 agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt
     7681 tctaattata ataagatgac cactacagta acctactgaa atatataaac agccactgta
     7741 gtaccatttt caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg
     7801 cggatatgaa tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag
     7861 gtactggtat caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa
     7921 agctgaatct tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg
     7981 agattgaaat atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa
     8041 cgatccttgg tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt
     8101 ctttactaac aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac
     8161 ttttatctaa gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt
     8221 atcagacgtg agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga
     8281 gcataatatg aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt
     8341 ctgaaattct ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt
     8401 tacaatgttt cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg
     8461 tatctaattc catggtatat caaccatttc ctatatagac tgattatgac atggattaaa
     8521 ataaaaaggc actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc
     8581 acctatagag gatgggcgac ttctgcctga aataagttac actacttata acttctaata
     8641 aaattagatt gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta
     8701 atactaattt gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct
     8761 ttgtactcac tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa
     8821 actttaagtc aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt
     8881 tttcagaagc tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg
     8941 ttgttttggc atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt
     9001 ctggctttcc aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa
     9061 agattgtcac tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt
     9121 gctccactaa aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta
     9181 cagattgaac ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga
     9241 gaccgttttc tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt
     9301 tatttttcca tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag
     9361 tattcactac cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg
     9421 tgaaattttt ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca
     9481 ccatttttcg atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt
     9541 ccttttgatt ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc
     9601 ggccagctcc gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg
     9661 gatctcctgc atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag
     9721 taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat
     9781 caatatgagt catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt
     9841 tggcataaca ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac
     9901 caaacacata ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt
     9961 tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc
    10021 cataattata ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt
    10081 tttgaccacc tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc
    10141 cgtttttccc gacatgatct agatacccgc caccatagtt ataggcctga ataatactat
    10201 cttgatcaca ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt
    10261 gtttgatgga tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt
    10321 gcataacgtc ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa
    10381 tatactctgt tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata
    10441 cttcttctga gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt
    10501 cttcaccaaa caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag
    10561 ttaatgaaat aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg
    10621 acgatggttc attctttttt gtactttttg attggtctgc tttggcagag tggacctctg
    10681 tttcacttgt tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat
    10741 ttcttgacta gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt
    10801 tggtgcttct tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat
    10861 attcgccctt ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc
    10921 cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc
    10981 ttggaacagt ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg
    11041 gagattgtat ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt
    11101 atctttcacc aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat
    11161 tcgggattgt ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc
    11221 gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga
    11281 ttttgattgt tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc
    11341 tcgtcccaca tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg
    11401 catgacgcgt cttcccattg actggctatc attactctgg aggttgaaca tactcataat
    11461 gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa
    11521 aaatggatag gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat
    11581 caccaaagta acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt
    11641 tcctaaactt tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc
    11701 aaagataatg aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa
    11761 ggaaatgcca atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt
    11821 ggtaatactg agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg
    11881 atctttgcct ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc
    11941 aatttcttct atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat
    12001 cgaaaacaaa ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg
    12061 tacaacgatc tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt
    12121 gatttttgtg atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat
    12181 cacaaaatta atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata
    12241 cgccacatac acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga
    12301 ggtctggagt ccattttcag taataccagc caatgtttgg atattctttc caatactttc
    12361 agccgtatcc gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt
    12421 cgacaaatac aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac
    12481 gcttttccct aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa
    12541 gaaatctagt tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt
    12601 atcatcaact agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag
    12661 agtgagtatg ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc
    12721 actccattct ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga
    12781 ctggatgtac ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat
    12841 tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt
    12901 ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat
    12961 tattcttcaa gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg
    13021 cattcatagc ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt
    13081 gaagaaaact ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga
    13141 taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg
    13201 tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga
    13261 tatttaactg cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat
    13321 gggcaaaatc ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg
    13381 agccttcttt tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa
    13441 ccgtccgaat ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg
    13501 ttaaaaaggt cagaatatca attgctagac tttcagaatc cttctttttc tccataatca
    13561 cataaggatc aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt
    13621 catccgcaat ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa
    13681 tcacagcttg tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact
    13741 ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaacccttga
    13801 ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac
    13861 caaagtaaat gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga
    13921 gaaaatcaga cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga
    13981 attcttcatg tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat
    14041 cataaaaatc tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta
    14101 ctcgaaccac atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca
    14161 attcatctac ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat
    14221 aggcatggtt gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct
    14281 ttttgttcgt cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt
    14341 gctggtaata gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg
    14401 ttaagtacgc tacatatgat tcatgattct catgttccat gcataagtac cggggctttt
    14461 cttcaatcaa acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact
    14521 tcagcttttt ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat
    14581 agatatgttc aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc
    14641 gagccaattt actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg
    14701 ataaggaaac aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc
    14761 cggagaagaa agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt
    14821 tgaagccaat aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct
    14881 ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt
    14941 ttgatcgttc ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct
    15001 tgccttcacg attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt
    15061 caggtgacaa aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct
    15121 cttgattaaa gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt
    15181 accagagtaa tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa
    15241 gtgaccttaa atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta
    15301 ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt
    15361 aaaaaggccc cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc
    15421 aaggataaaa caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca
    15481 ttaattgcat aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc
    15541 atcaaatcat tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt
    15601 gttaatatgg atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca
    15661 agtctctacc acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa
    15721 aatcaccagc ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat
    15781 aatagataat atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt
    15841 gaatctcaga agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca
    15901 accgattaat ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat
    15961 gaattgcata ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat
    16021 cgacaggtgg ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg
    16081 caatataaac acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa
    16141 acaaactgag taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca
    16201 aagcaatcac caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac
    16261 caatgaccac aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata
    16321 tggataggta agttcctgat attataaata tctcaaactt ttccccccat tcacacggag
    16381 cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag
    16441 tagacctata tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt
    16501 atgagctgga ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt
    16561 ttagcgtgta ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca
    16621 tttccaccag ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat
    16681 tcttccagag ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct
    16741 gcgtattcta ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta
    16801 tcaacactta gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa
    16861 tacacctttg ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc
    16921 tcgccagcaa cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta
    16981 agttgtctgc ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag
    17041 atagccatta cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga
    17101 taataatttt gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt
    17161 ttcccttttg agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt
    17221 ttgaattgtt tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt
    17281 ttaatcttga aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt
    17341 tcttgtgata cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct
    17401 ttttcagcac tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata
    17461 tacatttcct ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta
    17521 ccgcttttat tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt
    17581 tctacccaat ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc
    17641 ccttgcggag ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct
    17701 tttaatatcc tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc
    17761 tgttttatca gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc
    17821 acataatgta gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact
    17881 gacctattag gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct
    17941 aatacttgtt ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt
    18001 ctggttttac catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca
    18061 tgtggactgc ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg
    18121 ccaatatctt taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg
    18181 atatttcgat atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg
    18241 aaaatatctc cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa
    18301 tattcagcgt gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg
    18361 cttcactcct ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa
    18421 acaacataat acttttctaa tatatacacg acttgagggc attcctccat tctcattaca
    18481 agaatttctt aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg
    18541 tcagtttatt ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata
    18601 catatatatc cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta
    18661 tatggcattt cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt
    18721 tgcatttcta caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata
    18781 gaacattcta accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc
    18841 tggatagttt tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct
    18901 ttaccgagct tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag
    18961 gcgtttcaga gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca
    19021 aactttttcg ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa
    19081 ttttgtccga aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc
    19141 acttgcattc atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa
    19201 aatatctttt acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc
    19261 atactttgtt gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata
    19321 tcctggatat aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg
    19381 gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa
    19441 gttaattgaa tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc
    19501 cagttggttc gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg
    19561 gaaaacgtgg tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca
    19621 cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc
    19681 cctaacttga tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg
    19741 agactgccga tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc
    19801 ccggaacgat aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt
    19861 ttcgttagct caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt
    19921 ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga
    19981 gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct
    20041 ttatcttctt catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag
    20101 gagtaaaagg cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga
    20161 ataatttttc gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc
    20221 aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata
    20281 gatgcggtta cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc
    20341 gtagccaatt tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa
    20401 tctaaattcc gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg
    20461 agttaacacc tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc
    20521 ttacttattt tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc
    20581 ggctaacgcc gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc
    20641 cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt
    20701 acttccaact ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg
    20761 tccgcaatcc gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc
    20821 tcattcatta aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt
    20881 aaccgtcgca attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa
    20941 atttctccaa tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat
    21001 atcaaaaatg accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga
    21061 attgacttga gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc
    21121 tctaggtgtt agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt
    21181 tagtaaggta tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa
    21241 agccagattc acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat
    21301 catgattttt gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata
    21361 aactcgatta ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct
    21421 tgctggcagt gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga
    21481 accatagact tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat
    21541 gttttgatca gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc
    21601 tcgagaatgt ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt
    21661 aagcacctac cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa
    21721 cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg
    21781 tcggagaaga gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa
    21841 tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc
    21901 cactggtccg agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac
    21961 ctccttactt gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca
    22021 aaaaccgata caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc
    22081 gaataaatct aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca
    22141 ttttgctctt ggctcataca ttattaattt gtcctactat tttttattta atccaactaa
    22201 tttccgttgt ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt
    22261 tgcagtagca ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt
    22321 tacttgttct ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg
    22381 gtaaaaataa ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat
    22441 cttttattat gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct
    22501 ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa
    22561 tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg
    22621 tcactcgaaa atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa
    22681 aagttgcatc cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta
    22741 ccaaccaaga agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact
    22801 gggtgcgaaa gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa
    22861 tggcaacctc tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa
    22921 cttcggggga tttccctttg ataatataag aaataaactt atccttattt gcttttgcat
    22981 actctaaata agaatcatta ctttccatat taattatccc ctttcttcct actatataaa
    23041 agtattatac gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt
    23101 ttctaattta ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa
    23161 ctcactaatc acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa
    23221 gaagtttttt tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa
    23281 agccacgcga aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct
    23341 ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag
    23401 cttattcaaa atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc
    23461 atcaaagatt aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta
    23521 gttagacatt tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac
    23581 acaagcagaa atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc
    23641 taaatctgca tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc
    23701 gataatggtg aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg
    23761 gggtaaagaa tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc
    23821 cactacttcg tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac
    23881 ataggagtct ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt
    23941 ttccaatttt aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag
    24001 taatccatct ttcattcgat aatagatttt ggggaagtaa ctgatagtct ctctggttcg
    24061 gctactattc aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg
    24121 gttctctaga atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag
    24181 aaaaaagtga atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca
    24241 tactaaatct tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc
    24301 aaattgtcta aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag
    24361 ccagccaaga acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt
    24421 gtacattttg atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat
    24481 ttcaaaaagt tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca
    24541 attgatttaa aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg
    24601 attctgcctg ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt
    24661 ctcctaacca gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac
    24721 cttgatgtat aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct
    24781 cagtcagctc gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt
    24841 ccataatctt cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct
    24901 gccgcttcaa gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa
    24961 aaacataatt ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag
    25021 caaactcgcg taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag
    25081 aggattttaa attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt
    25141 gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat
    25201 ctacgacaat accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat
    25261 caacatacaa cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca
    25321 aatggggatt cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg
    25381 cagctactac cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc
    25441 gaccaatcac cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat
    25501 aacttaactt tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct
    25561 tttcctcttt tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc
    25621 ggtcgcgcac gttggttctt aggatttact tgtatcatct catcacctta gttccggcga
    25681 ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca
    25741 ttggaatttt tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag
    25801 ccacttggaa cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct
    25861 tttggtgtta aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc
    25921 ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg
    25981 gtatctactt ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca
    26041 atcttgccag atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt
    26101 gtttcgaaag cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac
    26161 atatccacca catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt
    26221 tggatagaaa catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta
    26281 acatccttcg ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag
    26341 ttgaataaga caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc
    26401 caagttcctg ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt
    26461 ttggcttttt catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct
    26521 accacatacc agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg
    26581 gtagaatcca gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa
    26641 ctatcttctt cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct
    26701 ttgttcgtca gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt
    26761 tcggtaatgg taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta
    26821 gaacggattt ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt
    26881 aaataatccc cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg
    26941 ttataagctg tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc
    27001 gacactttaa aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca
    27061 aatttaaaga aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta
    27121 atcacggcat ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca
    27181 tcaaggctat atccttcggg cgcttttgtt tcttgccaat aatactcgtt gatagctaaa
    27241 tgtttaacgg caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct
    27301 ttcgttccct tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc
    27361 ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc
    27421 ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct
    27481 tttacgttac tggtaacaag ggctacggtt tgattggcat acttcaattc gacttttaca
    27541 ggtttgaacg tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc
    27601 aattccaacg cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc
    27661 atttcttgaa cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct
    27721 aaggaataat tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg
    27781 atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg
    27841 gtggtagtca tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg
    27901 gttacttttg taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc
    27961 acgtcttttg caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca
    28021 tcacccgatt ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt
    28081 ttgattgtgt aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc
    28141 tttttatagg cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt
    28201 tcttttgaat tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg
    28261 tttgcggcat tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc
    28321 acagtagtcg attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc
    28381 tgataatcgt caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta
    28441 ccatctgatc gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc
    28501 tgtgccacaa tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat
    28561 ttggctttat cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg
    28621 taaatcggga ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca
    28681 taatgcggaa cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca
    28741 tattgatgtg ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca
    28801 aaagtaatcg taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt
    28861 aatacacata atttcttttt ttgtttttta tacatgaaat gttctccttt caaattaagc
    28921 acaaaaaaat aagatgctcg cttgaacatc tttgtttata aagtcttttt aaaattttta
    28981 ttgactgaat agaatggatt tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa
    29041 atatactgcc tttaaattag aacacatttt agaacacaaa catttcaaat ttaatcaaat
    29101 ataataaaat atatttacgg gaaaaccttt ataaatcaag gtttttcaaa cttattcaat
    29161 gatacgaaga aaccaggtct taagaaagca cgtaaagctt cacagttttc aaaacgttaa
    29221 tcgttgttat accaacgatt tcagacactt tctcattgcg agaaggtgtc tttttttcgt
    29281 tctggtaatg agaaaggtaa tgacaatatt ataaaccagt gtaagcaatg aatttttcta
    29341 acgcatttcg ttgagctttt tcagtaacat gagaataagt gttaagggta gtttcagcat
    29401 ttttatgacc caatcgatct tgtgtttctt tgatacttgc tccaccttct agtagaagag
    29461 tagcgtgagt gtgccggaat ccatgaatag taacgtgctt aaatttatat ttcttctcta
    29521 aacgtttcaa tatattattt gaatagtccg catgcaaagg ttgattaatg tcaccgtctg
    29581 catttacata ggtaaagata agttgatttt ttgtatttgt atttatgcca aactgcaaaa
    29641 gctctcgagc ttgttctttt ttccagattt ttaacatttc aatagtttgt gcatcaattg
    29701 gtaatattcg ttcggaagat atattttttg tatttttttg aatcctgaca gtttttcttc
    29761 ttgcattctt ctcttcattt cgaatagttg catagctctt tctaacatac attagctgtt
    29821 cttcaaaata aatgtcagac caatgaagag cagctagctc tcctttacgt ataccagtag
    29881 taatcaaaag ctggaagatt aaaatatgtt ttaaaggctc ttctttattg agaatatccc
    29941 aaaaagcttt tagttcttct aatgaaaagt aattttcatt ttctttcttt tcccgctttg
    30001 gtagatttgt atttgccata ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa
    30061 atatttgaga gctgtaagac ttaagctgtt tgatattctt gaattttaaa gcccattcat
    30121 tgactgcatt ctgacagtga acaggagtaa tcttagaaat ggacttattc ccgaattttg
    30181 gtagtatatg caagcggaaa aggtcttttg tacgttgata tgtcgtttct tgtacacgat
    30241 tcctataaga aggcaaccat tcttcataca ctgttataaa caagtcatta gaaggcttat
    30301 gatagccggt atcttcaatc tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt
    30361 cagttttaaa cccttttttt acatagtatt cttgctttcc attttcatct ttacgaacat
    30421 acaagcggac actatatcgt gcaccgcttt ttgttgtgta cttttgtatt gacataacta
    30481 atctctccaa tctgataact tggcgagttg gctgattgaa gaaatagaaa atgagaggag
    30541 aaaaataatg gatgcaggaa ttacagtaat gttagataaa caacaaacga atcaattgaa
    30601 aaattatgtt tttgaaatga caaaagaggc tattgatcaa gcaagaattg ataccggatt
    30661 agaaagacca tttctaaaag gaaaagagat ggctaaatat ttgaatgtat catatacaac
    30721 ttttttgaaa tttaaaagaa tgggattgcc agttatttta ttagaaaaaa tggagttgtt
    30781 ttcaaaagaa gaatgcaaaa aatggatatt gagtcatcaa atttaaaaaa cacctcgttt
    30841 tttaaatttg atgaagtaga aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg
    30901 ggaacactca ataataaagt tctgagaaaa ctttatttga atctattata gcaaataaaa
    30961 tcagaagcag gtgtgacaat gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc
    31021 tattctaatt ctgatatata tgcagaatta tactatcaat atcctatttt tttaaacgta
    31081 aatgaaaaat ataagaaaat gaaactaagt tctaagttag tttatatgtt actaaaagat
    31141 agaaatagac tttcattgaa aaataacttg tatgatgaga atcaaaaaat ttttttgata
    31201 tttgaaatca aaaatctcgt agaaatttcg ggaatgtcaa aaaatacaat cttatcagca
    31261 ctagatgaac ttcaaaaagt taaacttctt gtgaaaagaa aaaatggctt caataaaagt
    31321 aaaatgcaaa tgttcccaaa tttttattat ttgttaaagc ctgttctgtg caaagatgat
    31381 gtttataaaa ttaataagat aacccatcaa gaaaatagac aaaaaaactt gatagttcaa
    31441 aatttgaact atggaacaaa tcaatattct gttgatagtt caaaatttga actatgcgac
    31501 acatcaatga aaacaagaga caaaaacaat tttgctgata gttcaaaaaa tgaactagaa
    31561 ttatataagt cttataaaga tagtaaagat attaaagaat cacataatga tgatcttctt
    31621 gaatcacttt caactaaaaa taataaatta gatgaagaat tgatcgaaac atttattgaa
    31681 gaaaatcttc ttaaacaaat atggggagaa agtattgtga attgcttaaa actggctagc
    31741 aatagtgatt atcgtcaatt cgacaactgg tacaaaaaat tcaaatatgc gattagatct
    31801 gctgaaaaag aacaaaaaat tcaactgaaa attatctatg aaatttgtaa taacaagtat
    31861 tttgtagatc atgttagaga acagctatca atgactttac gtgacttgat acgaagagca
    31921 aaaacagatc atcgtatcaa acaaaaagac gattatatct ttgcttctct aaaaaataaa
    31981 gcgttagaac taattgaatt gcaaatcagc gaaggaatag ataagcaata agaatagaat
    32041 tgagtcaaat gaaatggagg ggtatctcca cttcatttga gaactttgaa tataggaggg
    32101 gttattttga ttaaaaatcg tttaatagat caagtttggt gtctagatca aaatacagat
    32161 agtctcgaat taatcgagac tatttgcttc aacactatag ttctcgattt aagagctgaa
    32221 aatgataatt gtgtaactca tgtcataatt aatcaaaaaa tggctcagaa gctctctgag
    32281 agcctcagaa aatgggctaa gggaggtaac gatgaaaata attgaaaaaa agattgccaa
    32341 taaaaaaata aattggttgg ttgttaattt tcttgttctg atagctatga ttatttttac
    32401 aagtaatatt gctcatgcga gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga
    32461 gtttttgaat aattctcttt atagacctta cctaaaaaag tacaatggag atttccttag
    32521 tcagttcggg atttggctcg gatgggctgt ggtaaaaggc atgtttactg tgacagattc
    32581 gattcaaaat atgattccag atgtgctgga tttgttcaat ttcattgaaa gtacagggct
    32641 aaataatgtt tatcaatcag ccgtaaatac aattgtcatt ggattaatga tcctttcatt
    32701 gatgtttgtt ggttacaaaa tgattactgg aaaaggaaca gtggatttaa aatcggttgg
    32761 aatgaacgta gtgatgtcta tagcattaat acttttaatg ccaaccatga tttctagtgg
    32821 tattcaattt gctaaaacat tttataagga ctctactacg attacaaatt cagatgacgg
    32881 agtcgcttgg tcattgataa aacaaggagt aacagattta gcatatatta ataaaacaga
    32941 tcagtactca agcattgata aaacggagga tcgaaataat ttaacacgaa aaaattttca
    33001 acagacggat ctcacacaag tgttaacgga taaagtgatt gataaattgg aaaaagaaaa
    33061 tccaattgcg gacaatcttc gttatgaatt agttgaaaat tctaataatg aatttgttgc
    33121 tacaaagttt tctgacaact tcttatcaac attttctgat agcttgaaat caggctatta
    33181 ccgataccaa gcaaatctat gggggatttc aattgggttg acagcgttag caatcgccta
    33241 tgtgttcagc gcgtttgtga tcattacagc aatactagaa cttgcattta aacgtgtgtt
    33301 aggtgttctt gtttttgcaa cagatattga gacaggacag cgttcaaaag ttgttctatc
    33361 agatattcta caatgttact taactgttgg gtttcaagga tttgggttat caatgtttgc
    33421 tatgtttatc aattttctga actcaggaca aggtatctca acaaatattt ttataaaaac
    33481 aattgcttat atctgtgccg tttttgtttt aatcaaaggc agtggaacag tcatgagata
    33541 ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggaat
    33601 gggcgcaatg ctgtttagaa aaggatcaaa tggatttaat cgtgctaaag gaagcggaga
    33661 aggagaagat cgaaaaccag aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa
    33721 aactggaaga gctttgggat atgcccatga acgtggatta tccggtttag cttcagatgg
    33781 agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg atatagctaa
    33841 tgataccaag aataagttta aagaaggttt agatgatggt acagtgtccg ctattaataa
    33901 aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat atgctgattc
    33961 cgtgccaaca cgtctttcag atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat
    34021 tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa
    34081 tcctgcaagc gcaattcagc aaaaagtcca acaagatatt gaagaacgta aaaatgctat
    34141 gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgtcatg aagcgaaata
    34201 cacgcctgaa gcaatgaatc gcgaggaaat aatgttaagg aaacgtgtag aagggcggac
    34261 aggcgctaat attgacggaa aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa
    34321 ttcaaataca ggattagaaa aattagtcaa agaaaatcca caaaattcta caagtagtca
    34381 tggtggccga tcagtagatg tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg
    34441 acggaatgtt gatgtaagag agaatttaca aagttcttca aatgctcaag gtactaagac
    34501 aattgatgtt cgagaaaatc tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt
    34561 tcgagaaaat gtacaaaatt ctacaagtag tcaaggattc aaaacagtag atgttcggga
    34621 gaac
//
LOCUS       DACEKQ010000021        32662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEKQ010000021 DACEKQ010000000
VERSION     DACEKQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.022"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..391
                     /locus_tag="IW671_000915"
     CDS             161..391
                     /locus_tag="IW671_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0897917.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IW671_000916"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IW671_000916"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0897918.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IW671_000917"
     CDS             1802..1978
                     /locus_tag="IW671_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0897919.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IW671_000918"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IW671_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0897920.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2594..3061
                     /locus_tag="IW671_000919"
     CDS             2594..3061
                     /locus_tag="IW671_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0897921.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="IW671_000920"
     CDS             3146..4030
                     /locus_tag="IW671_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0897922.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="IW671_000921"
     CDS             4147..4380
                     /locus_tag="IW671_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0897923.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="IW671_000922"
     tRNA            4468..4548
                     /locus_tag="IW671_000922"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="IW671_000923"
     tRNA            4555..4626
                     /locus_tag="IW671_000923"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            5141..5848
                     /locus_tag="IW671_000924"
     CDS             5141..5848
                     /locus_tag="IW671_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0897924.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IW671_000925"
     CDS             complement(5907..6551)
                     /locus_tag="IW671_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0897925.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="IW671_000926"
     CDS             6677..6964
                     /locus_tag="IW671_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897926.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="IW671_000927"
     CDS             7072..7392
                     /locus_tag="IW671_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897927.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="IW671_000928"
     CDS             7473..8786
                     /locus_tag="IW671_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0897928.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8783..9502
                     /locus_tag="IW671_000929"
     CDS             8783..9502
                     /locus_tag="IW671_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0897929.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="IW671_000930"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="IW671_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0897930.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="IW671_000931"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="IW671_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0897931.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13063..14164
                     /gene="prfB"
                     /locus_tag="IW671_000932"
     CDS             join(13063..13125,13127..14164)
                     /gene="prfB"
                     /locus_tag="IW671_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0897932.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14269..14955
                     /gene="ftsE"
                     /locus_tag="IW671_000933"
     CDS             14269..14955
                     /gene="ftsE"
                     /locus_tag="IW671_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0897933.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14939..15832
                     /locus_tag="IW671_000934"
     CDS             14939..15832
                     /locus_tag="IW671_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897934.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15984..17432
                     /gene="cls"
                     /locus_tag="IW671_000935"
     CDS             15984..17432
                     /gene="cls"
                     /locus_tag="IW671_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0897935.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNIGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17769..18626
                     /locus_tag="IW671_000936"
     CDS             17769..18626
                     /locus_tag="IW671_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0897936.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18741..19661
                     /gene="pstC"
                     /locus_tag="IW671_000937"
     CDS             18741..19661
                     /gene="pstC"
                     /locus_tag="IW671_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0897937.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19661..20545
                     /gene="pstA"
                     /locus_tag="IW671_000938"
     CDS             19661..20545
                     /gene="pstA"
                     /locus_tag="IW671_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0897938.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20560..21366
                     /gene="pstB"
                     /locus_tag="IW671_000939"
     CDS             20560..21366
                     /gene="pstB"
                     /locus_tag="IW671_000939"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897939.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21382..22140
                     /locus_tag="IW671_000940"
     CDS             21382..22140
                     /locus_tag="IW671_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897940.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22153..22830
                     /gene="phoU"
                     /locus_tag="IW671_000941"
     CDS             22153..22830
                     /gene="phoU"
                     /locus_tag="IW671_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0897941.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23067..24098
                     /locus_tag="IW671_000942"
     CDS             23067..24098
                     /locus_tag="IW671_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0897942.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24095..24862
                     /locus_tag="IW671_000943"
     CDS             24095..24862
                     /locus_tag="IW671_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0897943.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24962..26530
                     /gene="liaX"
                     /locus_tag="IW671_000944"
     CDS             24962..26530
                     /gene="liaX"
                     /locus_tag="IW671_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0897944.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSVIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26830..27147
                     /locus_tag="IW671_000945"
     CDS             26830..27147
                     /locus_tag="IW671_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0897945.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27147..27497
                     /locus_tag="IW671_000946"
     CDS             27147..27497
                     /locus_tag="IW671_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0897946.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27664..28602
                     /locus_tag="IW671_000947"
     CDS             27664..28602
                     /locus_tag="IW671_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0897947.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28617..29447
                     /locus_tag="IW671_000948"
     CDS             28617..29447
                     /locus_tag="IW671_000948"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0897948.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29491..30516
                     /locus_tag="IW671_000949"
     CDS             29491..30516
                     /locus_tag="IW671_000949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0897949.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30531..31469
                     /gene="galU"
                     /locus_tag="IW671_000950"
     CDS             30531..31469
                     /gene="galU"
                     /locus_tag="IW671_000950"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0897950.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31594..32034
                     /locus_tag="IW671_000951"
     CDS             31594..32034
                     /locus_tag="IW671_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0897951.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32037..32639
                     /locus_tag="IW671_000952"
     CDS             32037..32639
                     /locus_tag="IW671_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0897952.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10843 a         5541 c         7079 g         9199 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgacgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttaatgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtatttcagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgataaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggcgc gctaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattccg attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gggtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaaggtt gtgaaaaata cggctagcac
     2521 tgagcgataa agaaaaagag gaaatttatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag taaccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag tgattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaaatttc aattcaagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caacttccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gcgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttacggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggggttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatatt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atggaccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccgggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgga catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcacgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcacaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc gtattagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agaccgcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtatgcccgc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttcg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcgatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tcttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa ttgctgtagt atgcgataat aaagacaaag
     6661 gcaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tctgatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagacgtg gatgtgttgt tattaggccc acaagttcgc
     7261 tttatgaaag ctgattttga gaaacgtctg gaaccaaaag gcattccttt agatgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg agcaactgga ttctttgtca tcgctgtgga atgagaacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc tcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggagatcaa gcagacgttt gacatccaca aacttgcttt acgctttcat cggcgtttgc
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatatt
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagata ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaattct atccccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcagatc gcacagccga aaatcatccg
     9481 aactatttct ttggcgagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcaaatgctg
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttgcaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttaggtttat atccatacca cgtacaattg atgggtggaa tcgtgcttca
    10681 cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc
    10741 agtatatcta aatgctctga ctggcgaagg ggtacacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga actttataat ttcttaggat taacagtggg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gtgatatcac
    10921 ttatagtacg aataatgaat taggatttga ctacttgaga gacaatatgg tcgtttaccg
    10981 ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcaatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tgtacaatca aaaacaatca gtttaaccga agcagggatc gaaaaagcag aagaaacatt
    11221 cggtttggat aacttatacg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactatatca tgcttttaga tatcgattat gttgtccaag acggaaaagt
    11341 actgattgtt gaccagttca ctggtcggat catggatgga cgccgttatt cagatggact
    11401 tcaccaagca atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaaccatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggaa tgactggtac
    11521 agctaaaaca gaagaagaag aattcagaga aatttataac atccaagtat accagattcc
    11581 gacaaatcgt ccggttatcc gtgatgatcg tccagatttg ctttacccaa cattacaaag
    11641 caagttccac gctgtcgtgc aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt
    11701 agtcggaact gttgcggttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtgctga atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa
    11821 tgctggtcaa aaaggtgctg tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagttagga cttggggtaa gagaacttgg tggtctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacag ttctacttgt cattggaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaagct ttcttagatc ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa
    12121 gatgctgacg aaacaagtag aatctgcaca aaaacgtgta gaaggaaata actatgacac
    12181 acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa caacgtgaag taatctataa
    12241 acagcgacaa gaagtaatca tggaagaaaa aagcttatcc aagccactaa tgaacatggt
    12301 caaacggaca atcagtcgtg tagtagatgc gcatacacaa ttagagaaag aaaactggaa
    12361 tttggaaagt atcatcgatt ttgccggcag tactcttgtc catgaagata gtattactct
    12421 tgcagatatc gaaggaaaaa cagctcagga gatcaaagag tatctaaacg aacgagcaca
    12481 agaagtattt aataccaaag cggctcaact aaatggtcca gagcaactgt tggaatttga
    12541 aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca gaccatattg acgcaatgga
    12601 tcaattacgt cagtcaatcg gcttacgtgc ttatggacag aacaatccat tagtcgaata
    12661 tcaaacagaa ggctataaga tgtttgaaga tatggtaggc gccatcgagt atgaagtcac
    12721 tcgaatcttt atgaaagctg agatccgtca aaacgtacag cgtgaacagg tttctcaagg
    12781 aacagcttct cactcagaag acggagatgc caaagaaaat agtaatacta gcgcgaaaaa
    12841 acagccaatc catgtgaaca aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcatg gaaaaaatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt tttttaagaa ttcgtctcac cttctttctg ttatttgctg attctgttag
    13021 actagatata atgaaaaaag tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt
    13081 cgaaatctcc tgacagaaat gcaggaaaag gtcacaagtt tcaggaggtc tctttgactt
    13141 agatcagctg gaagaagaca tcgcggaagc agaagcaaaa atggctgaac ctggtttttg
    13201 ggatgacagc gaagccgcac aaaaagtgat cagtgaaaat aacgctaata aagaaaccta
    13261 tgaccaattc cattctcttg ccgaagaact agaagaaatc gaattgatgg tggagatgct
    13321 gcaagaagaa cctgatgcag caatgcaaga agaagcagaa acacgtatca atgcattaga
    13381 tgaaaaaatg aagacgtatg aattatctat gctgttagac ggaccttatg atcgaaacaa
    13441 tgcgatcgtc gaattgcatc caggggctgg cggaacggag tcccaagact ggggcagcat
    13501 gcttttacgt atgtatacac gttgggcaga gcagcatggc tatcaagtag aaacactaga
    13561 ttatcaagca ggtgatgaag cagggatcaa gagtgtgacc ctgctgatta aaggacataa
    13621 cgcttttggt tatctaaaat cagaaaaagg tgttcatcgc cttgtacgga tttcgccttt
    13681 tgactcagcg aaacgacgtc atacttcttt ttgttcggta gatgtgatgc cagagcttga
    13741 tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa atcgatacgt accgagcaag
    13801 tggtgcaggt ggtcagcaca tcaataaaac agaatctgct gtacggatca ctcacttgcc
    13861 tacaggaaca gtagtagcta gtcaggcaca acgctcacaa ttgaaaaacc gtgagcaagc
    13921 gatggggatg ttaaaagcta agctttatca attagaagtg gaaaagaaag aacaagaagc
    13981 agctgcactt cgaggagaac aaaaagaaat cggctggggt tcacagatcc gttcttatgt
    14041 tttccatccc tattctatgg taaaagacca tcggacaaac gtagagacag gaaatgtcca
    14101 agcagttatg gacggtgacc ttgatttatt tattgacggc tatttaaaaa tgcatctaca
    14161 ataagaaccc attgttaaat tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat
    14221 tgccatgcta taatagctaa agactgaaaa agatatggag agaacgccat gattgaaatg
    14281 aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta tccgcaatct ctcgattgtc
    14341 atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg gagccggtaa atctacattc
    14401 atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat
    14461 gatctaatag caatcaaaaa ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg
    14521 ttccaagact ataaattatt accaagaaaa acagtatatg aaaatgttgc gtacgctatg
    14581 caagttatcg gcagaaaacc tcgagatatc aaaaaacgag taatggaagt gctggacctt
    14641 gttggtttga aacacaaagt tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa
    14701 cgtgtctcta ttgctcgggc aatcgtgaat acgcctaaag tacttatcgc ggatgaaccg
    14761 acaggaaatc tggatccaga aaactcatgg gaaatcatga aattattaga tcggatcaat
    14821 gcccaaggaa ctactgtcgt gatggctaca cataacagta cgatcgtaaa tacgattcgg
    14881 caccgagtaa ttgctatcga aaacggccgc attattcgag atcaagcgga aggggaatat
    14941 ggatacgatg attagaacct ttttccgcca tttattagaa agtatcaaga gtttgaaacg
    15001 taacggctgg atgacagttg cttcagtcag tgcggtaacg atcacgttga ctttagtcgg
    15061 tgtattcatg gcagttatca tgaatgcaac taaattagct caggatatag aaggaaacgt
    15121 tgatgtatct gtattcgtag atatcggtac aaaagacgaa gagatgaaaa agctagaaaa
    15181 agaattgaat gacttagatc atgttaaaaa agttgaattt tcaagtaaag aacaggaatt
    15241 gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg tttgaaggtg acaataaccc
    15301 gttatatgat gtgtatgtag taagtgctac tgatccttct tatacaaaac aagtagcaga
    15361 agaagcagca gatttgaaga atgtttctcg ttctgattac ggtggagctt cctcagatcg
    15421 gattttccaa atcgctggtg cagttcggac atggggatta gttgcagcgg cattgctgct
    15481 gttcgtagcg atgtttctca tttcgaacac gattcggatc acgattttgt ctagacagcg
    15541 tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga tatattcgct ggccgttttt
    15601 ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt ccgattttga tcatcacatt
    15661 tggttatgca tggtttttcc aattgatcaa tccatcatta ttacgttccc attattcatt
    15721 gattcatcca caaaatatcg tttggaaaat caatctttta atggtcggca ttggtgtgat
    15781 cattggttca cttggctcga tcatttctat gagacgtttc ttgaaattct aataccacaa
    15841 tataaataaa atgagaaagt tggacagttt cgttccagct ttcttttttt attggctagg
    15901 agaatagaag gaagcggctt ttttcccttt actttaaaaa acaacttggg tattcttaag
    15961 ttggtagagg aagaggtgaa gccgtgaaaa ttatcgttga caattttttt acgatccttt
    16021 tgattatgaa tattttgctt tcctttatca tcgtttttcg agaaagaaag gaaactgctc
    16081 aaacatgggc atggctgctc gttttgatgt tcattccagt tgtaggattt attttatata
    16141 tttttttagg aagagggatt tccaaagaga aaatctttga tttgaagatc caagggaaaa
    16201 tcggtaagaa cctggaaatt gaagaagaca aacaggcatt gatgcgaggg ctttatcctc
    16261 atccgcctac tggacaagta gatgtgaaac aattgatcta tatgctcacg gtatttgaaa
    16321 gtacgctcta tacgactaaa aatgaaatca ctttatttac agatggtcgt gaaaaattcg
    16381 atgcgctgat tgaagatatc aagcaagcag aagaccatat ccacttccag tattatattt
    16441 atcgcagtga tgcattaggt gaagaagttc gagatgcatt gacagaggcg gcaagacgag
    16501 gggtaaaagt cagagtcctt cttgatgcat ggggttccac acaagtttct tccagctttt
    16561 ttcagaattt gaaaaaagca ggcggagaaa tcgcattttt ctttcctcta tttgttccgt
    16621 atattaatcc tcggatcaat tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa
    16681 tcggatatac aggtgggttc aatattggaa atgaatatct aggattggta aagaaatttg
    16741 gttattggcg agacaatcat ttgcgtattt acggagaagc ggtgtatatc ttgcaaaacc
    16801 gttttttgat ggactggaat tctcagcata caaaagaagt aggctattct cccaaatttt
    16861 ttccgtccat tcactctact ggtgatatag ctgttcaaat cgtgacaagc ggaccagata
    16921 ccgaacatga acaaatcaaa atgacttatt tgaaaatgat ttcgttagcc aaacgggaga
    16981 tattgatcca aacgccttat tacataccgg atggatcgat tcatgaagca ctgaagttgg
    17041 ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa caaaccagat catcttctcg
    17101 tttactgggc aacgtattct ttcgctgcag agctgatcga gtatggagca aggatcgaaa
    17161 catatgagaa tggatttatt catgcaaaga cgatgattat cgatggtggc attgtttcag
    17221 ttggctcagc aaatatcgat gttcgttcat ttcgcttgga ctttgaggtc aatacactga
    17281 tttatgatga acgaatggct tcacgtgtaa gaaaagcatt ttttgaagac tcgaaaattt
    17341 ctacgcatct cacaaaagaa atgtatgaaa accgaggaat aatcatcaag atgaaagaag
    17401 gattagcaag attgatttcg ccgctccttt aaaacacaaa aaggacatga gaaccatcaa
    17461 cagatggaga tctcgtgtcc ttttctaacg gggccatttc tgagagtacg tcagaaatgg
    17521 agaaagcgtc tcgcatgagc gaaattaact ttggggtatc tcttgttttt tggggaaagc
    17581 aagaaataag ctaatatatg ctattaaaag agacacattt caaagccagt atagcataga
    17641 aacttttaat gagatagttt tttttacaat tatgaggatt tattttgtta tttaatgatt
    17701 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
    17761 gatttctaat gaaaaaactt aaattattct taccaataat aggattagct attcttttat
    17821 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
    17881 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
    17941 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
    18001 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
    18061 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
    18121 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
    18181 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg tgcttcagga agcggaaccc
    18241 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
    18301 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
    18361 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
    18421 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
    18481 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
    18541 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
    18601 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
    18661 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
    18721 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
    18781 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
    18841 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
    18901 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
    18961 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
    19021 ttttatctgc tattgttgcc acaccatttg ccattggtgc agcagtattc atgacggaga
    19081 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
    19141 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
    19201 ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
    19261 cgactgtcac aacgatgact gttgatgcat taaaggcagt accacggcac tatcgggaag
    19321 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
    19381 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
    19441 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt actactcctg
    19501 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
    19561 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
    19621 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
    19681 taaaattgct acaggtgttt tatatgtagt ttcggggatc atcgtattga ttttagcagc
    19741 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
    19801 accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta actcgattta
    19861 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
    19921 ttcagaatat gctggaaaaa actggctgac agatgtgatt cgcacgtcga ttgaaatctt
    19981 gagctcattg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
    20041 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
    20101 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
    20161 attggcttta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc cagaagcgac
    20221 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
    20281 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
    20341 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
    20401 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
    20461 cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
    20521 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
    20581 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatcta
    20641 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
    20701 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
    20761 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
    20821 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
    20881 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
    20941 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
    21001 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
    21061 gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga tatcttgctg
    21121 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
    21181 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcggct
    21241 cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata tgacaaaact
    21301 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
    21361 ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga tcttcatctg
    21421 tattatggaa agaaagaagc actaaaaggg atcgatctaa gtttcaatca aggggaaatc
    21481 actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc tttgaaccgg
    21541 atgaatgacc tgattccgaa tgtaacgatc accgggagtg ttttatataa agacaaagac
    21601 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
    21661 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
    21721 ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
    21781 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggtgggcag
    21841 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
    21901 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
    21961 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
    22021 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga tacgaagaag
    22081 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
    22141 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
    22201 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
    22261 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
    22321 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
    22381 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
    22441 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
    22501 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
    22561 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
    22621 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
    22681 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
    22741 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
    22801 ttaaatacga accacaatga gattttctaa actaaagcgg tgattctgcc gtttgtttag
    22861 gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag taataagaac
    22921 agtcaaacaa aaatagctcc tcatattttt catttggctg gacttattac cgcagatgca
    22981 agcttttgaa cactgtttat tcgggaaagg gtttgtgaca aaatatgtca caaacccttt
    23041 tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt
    23101 ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat gaagtacctg
    23161 tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca ggaagactga
    23221 tgaacacgaa tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa
    23281 tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat
    23341 ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg
    23401 gcgccatgtt gcttattggc ggaaaacatg gtctatttgc tttaggaagt ctcatcatca
    23461 atatcgtttt acttttactt ttgttgctca tctttactaa aatagaaggg atttctctat
    23521 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
    23581 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
    23641 tttttatttg tcttttgaca atgaatcttt ggaaagacca aggactgcgc tatgaggaac
    23701 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
    23761 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
    23821 gacaaccaga aataacagcg aaacagttat ggctttcggg aaggcaagtg ggtcgagatg
    23881 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
    23941 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
    24001 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
    24061 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
    24121 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
    24181 cttattcatc aatttaggga tctttttctt gttgttattc ggttatcatc agaagtggcc
    24241 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
    24301 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
    24361 tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta caacgataga
    24421 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
    24481 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag
    24541 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
    24601 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
    24661 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
    24721 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
    24781 cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
    24841 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
    24901 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
    24961 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
    25021 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga
    25081 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
    25141 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca
    25201 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
    25261 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
    25321 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
    25381 attagcacag cgtcagacgt tagaagcaga aatcaaagag ttagaagcta gtggcgatac
    25441 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
    25501 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
    25561 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
    25621 aaaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg
    25681 gaaagatgtc agcttgcgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta
    25741 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
    25801 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
    25861 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
    25921 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
    25981 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
    26041 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
    26101 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
    26161 cttagattct gttatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
    26221 cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
    26281 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
    26341 tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga
    26401 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
    26461 tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
    26521 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
    26581 aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta tctcttgaac
    26641 accgtttacc aagagattat gaacttgcca attatcgccg agtcacaagg aataatttca
    26701 aaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcaaaaaga
    26761 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
    26821 tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt gacagggact
    26881 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
    26941 ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt agctttgatt
    27001 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
    27061 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
    27121 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
    27181 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga
    27241 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
    27301 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
    27361 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
    27421 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac
    27481 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
    27541 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
    27601 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
    27661 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
    27721 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    27781 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    27841 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    27901 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa
    27961 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    28021 cgcttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    28081 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    28141 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat cgcagacgat
    28201 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    28261 gaacatctga ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    28321 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    28381 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtaggc
    28441 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    28501 gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    28561 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    28621 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    28681 tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag gctgttcggg
    28741 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    28801 ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    28861 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    28921 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    28981 ctgcaccaag tgtattatta gcacaagcaa tcggtcgttg gggaaatttc atgaaccacg
    29041 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    29101 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    29161 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    29221 aaaaaggtga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    29281 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    29341 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    29401 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    29461 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    29521 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    29581 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    29641 ttaccagatc attgtctacc cgattccatc caagcattca ccaatatgga agatgcagta
    29701 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    29761 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    29821 caggggacac ataaaaggat ctctgacatc ttgttagagg aaatcccagt agaaaaaaga
    29881 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa acatgacatc
    29941 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    30001 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    30061 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    30121 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    30181 atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct agtcgttact
    30241 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    30301 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca
    30361 gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac aatctatgaa
    30421 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatggg
    30481 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtca
    30541 aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta
    30601 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    30661 cgttgaaatc aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    30721 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    30781 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    30841 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat
    30901 ttgtcgttat gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa
    30961 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    31021 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    31081 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    31141 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    31201 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    31261 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    31321 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt
    31381 ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    31441 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    31501 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    31561 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg
    31621 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    31681 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    31741 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat
    31801 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    31861 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    31921 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    31981 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg
    32041 ctaaaaaagg tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg
    32101 cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg
    32161 atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt
    32221 taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag
    32281 gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata
    32341 tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag
    32401 aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata
    32461 tcataattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt
    32521 cagaagcaaa accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg
    32581 cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa
    32641 atcacaaatt tatagaataa aa
//
LOCUS       DACEKQ010000022        32240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEKQ010000022 DACEKQ010000000
VERSION     DACEKQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.023"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            298..1959
                     /locus_tag="IW671_000953"
     CDS             298..1959
                     /locus_tag="IW671_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0897953.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2029..2376)
                     /locus_tag="IW671_000954"
     CDS             complement(2029..2376)
                     /locus_tag="IW671_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0897954.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2460..3401
                     /locus_tag="IW671_000955"
     CDS             2460..3401
                     /locus_tag="IW671_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897955.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="IW671_000956"
     CDS             3402..4445
                     /locus_tag="IW671_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0897956.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="IW671_000957"
     CDS             4451..5521
                     /locus_tag="IW671_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897957.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="IW671_000958"
     CDS             5511..6464
                     /locus_tag="IW671_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0897958.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="IW671_000959"
     CDS             6476..7486
                     /locus_tag="IW671_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0897959.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="IW671_000960"
     CDS             complement(7771..8592)
                     /locus_tag="IW671_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0897960.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="IW671_000961"
     CDS             complement(8689..8967)
                     /locus_tag="IW671_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897961.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="IW671_000962"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="IW671_000962"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0897962.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRC
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="IW671_000963"
     CDS             complement(11723..12310)
                     /locus_tag="IW671_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897963.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGFLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="IW671_000964"
     CDS             12485..13807
                     /locus_tag="IW671_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0897964.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="IW671_000965"
     CDS             13962..14738
                     /locus_tag="IW671_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0897965.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="IW671_000966"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="IW671_000966"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0897966.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="IW671_000967"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="IW671_000967"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0897967.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="IW671_000968"
     CDS             16628..17257
                     /locus_tag="IW671_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897968.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IW671_000969"
     CDS             complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IW671_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0897969.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="IW671_000970"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="IW671_000970"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0897970.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19444..19980
                     /locus_tag="IW671_000971"
     CDS             19444..19980
                     /locus_tag="IW671_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0897971.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="IW671_000972"
     CDS             complement(20020..21783)
                     /locus_tag="IW671_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897972.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="IW671_000973"
     CDS             complement(21786..23501)
                     /locus_tag="IW671_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0897973.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24280..24504)
                     /locus_tag="IW671_000974"
     CDS             complement(24280..24504)
                     /locus_tag="IW671_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897974.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24704..27472
                     /locus_tag="IW671_000975"
     CDS             24704..27472
                     /locus_tag="IW671_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0897975.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27553..27972
                     /locus_tag="IW671_000976"
     CDS             27553..27972
                     /locus_tag="IW671_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0897976.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27984..28454
                     /locus_tag="IW671_000977"
     CDS             27984..28454
                     /locus_tag="IW671_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0897977.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28471..29250
                     /locus_tag="IW671_000978"
     CDS             28471..29250
                     /locus_tag="IW671_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0897978.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29237..30058
                     /locus_tag="IW671_000979"
     CDS             29237..30058
                     /locus_tag="IW671_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0897979.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30075..31124
                     /locus_tag="IW671_000980"
     CDS             30075..31124
                     /locus_tag="IW671_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0897980.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31137..32141
                     /locus_tag="IW671_000981"
     CDS             31137..32141
                     /locus_tag="IW671_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0897981.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        10501 a         5734 c         6576 g         9429 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaagtgt tgatttttta ataattattc attaaactct
      241 taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt acgtaaaatg
      301 aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc tggatgttat
      361 ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga tggaggaggc
      421 gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt tgcaacagat
      481 acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct tgatgaagac
      541 agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga cggattaact
      601 tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg
      661 gattacgttt atggctggca acgaacagta gacccagcta cagcttctga atatgcttac
      721 ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga taaatcagaa
      781 ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa acaaacacca
      841 tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc cgtagtggaa
      901 gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg accattcaca
      961 ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa aaatgatcat
     1021 tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt aaaagaatct
     1081 tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt gtctggtgag
     1141 ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc gcggacaagc
     1201 tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa
     1261 gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg tgacggatca
     1321 gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac gaacgaagac
     1381 tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa agaatattgg
     1441 gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat ggcttctgat
     1501 gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga aaacttggat
     1561 ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg ctcaacttcg
     1621 ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc aagcagcttt
     1681 actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa tgcagaatat
     1741 gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc ccgttgggat
     1801 gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa
     1861 aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc
     1921 gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc gtgagcatga
     1981 gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt atcctaacaa
     2041 agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga tcagccaaaa
     2101 gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt tctttcggtt
     2161 ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac
     2221 ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat cattagataa
     2281 ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca gaatcagtag
     2341 aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttgttatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caagaagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt cattcatagg
    23701 aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata tatcaagtaa
    23761 atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa tcttttttgc
    23821 tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca aagaaggagt
    23881 atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag gaataaataa
    23941 tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac gaagacaacc
    24001 tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa aagaaaacaa
    24061 ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa ctaagagtgg
    24121 tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct gaaagcatca
    24181 taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata aggatttgta
    24241 atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat ggaaggattt
    24301 atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta tatattgcgt
    24361 atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag gcaaaaatac
    24421 aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag tatgtttcct
    24481 gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt tcatatgttg
    24541 aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa ttataaggga
    24601 taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa gattttattg
    24661 acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga agaaggaaat
    24721 tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga acgaggtctt
    24781 tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca gtcattattt
    24841 gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag tgatttattt
    24901 ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt atttttctat
    24961 cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat tctcttttat
    25021 gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag ctctttacta
    25081 tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta aaagttattt
    25141 agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt ctgcaccatt
    25201 catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta gcaatttgtt
    25261 tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg cttttgaagc
    25321 agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag aaggtcagga
    25381 aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac ctaatttgtc
    25441 acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta gtcatttctt
    25501 aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta atcagcgtag
    25561 tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa gaaaaatggg
    25621 gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga aattatccgg
    25681 aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat atgctgaaca
    25741 gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac tacttgcgca
    25801 gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc agaccttaga
    25861 gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt ttgagcaaat
    25921 cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa aattaaagca
    25981 agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag aaaaacacga
    26041 gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg aaactgccta
    26101 tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct atcagcgaag
    26161 cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag aattagaaca
    26221 acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc tggaattaag
    26281 taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga cgatctatct
    26341 aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg ctgctcacgg
    26401 ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag aggatctatt
    26461 tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag aaatccttga
    26521 ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca tgggatctct
    26581 gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga ttttgaataa
    26641 tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg tatcattaga
    26701 agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc tctatccaga
    26761 ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta cggcggtcca
    26821 cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa tcatcccata
    26881 tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca tatacgaagt
    26941 cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc tttcattaga
    27001 agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca ctactatgaa
    27061 tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat tggaaaaagt
    27121 actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg atcattttat
    27181 gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat tagcgttgta
    27241 tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg aaacctatca
    27301 aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta aatctgcgtt
    27361 tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg cctatatcta
    27421 tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt gaacataagc
    27481 aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta agtgaaaaga
    27541 ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt tgcttcaggg
    27601 atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt attcgattgc
    27661 tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat gaggtacaaa
    27721 gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa cgaattctta
    27781 caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc actattgatt
    27841 gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca gcaggtgcta
    27901 actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa aatagaagaa
    27961 gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat cggttattac
    28021 acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt ttgattgcta
    28081 atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc aagccacaag
    28141 gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa agcggggtga
    28201 cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat aagctggcag
    28261 aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa cctggtactc
    28321 gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg aaacgattaa
    28381 ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa gtggatgtaa
    28441 gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt attaggaatt
    28501 gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc tcgtccgatt
    28561 gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat cattatgggc
    28621 gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat tccacctgat
    28681 gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc cggtacagaa
    28741 accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa aaatatttac
    28801 ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga agaaggaaat
    28861 tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt gatgcttggt
    28921 gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct attaaatatg
    28981 attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc agctcttggt
    29041 tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt tttcttaggt
    29101 tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt tggtgcgatt
    29161 ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca aacaactgca
    29221 ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa cgaaaaaaga
    29281 gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt atgaacgtca
    29341 gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat atcagtcgaa
    29401 agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga ccccacatat
    29461 cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg atgatcctga
    29521 atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc ttgcagggtt
    29581 aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg gtacctcttt
    29641 agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca atattccgca
    29701 cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg gttttttgaa
    29761 aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta ttattggact
    29821 gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt taaaaatcgg
    29881 ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc caaatatctt
    29941 atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga aaccattaac
    30001 gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga ttttttaaaa
    30061 aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag gaagtactaa
    30121 gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa acagtgaatt
    30181 tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag ccagcattgg
    30241 aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc aattatggaa
    30301 aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa agtgcttcta
    30361 ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct atcacaaatg
    30421 acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt gccggggtag
    30481 aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta ctactcttag
    30541 ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa gactacatgc
    30601 aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg gaagaatttt
    30661 ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt ggatatggtc
    30721 caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc cgcgttccgt
    30781 cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca gatgcttcgc
    30841 atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa aagttgcaaa
    30901 gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa gcaagaaacg
    30961 agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta gcgcttgtcg
    31021 ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac ttgaacaaaa
    31081 agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg gaaatcatgc
    31141 tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct ttacgtccgc
    31201 aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc tattatctag
    31261 gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat gggaaaagca
    31321 acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac aaaagattaa
    31381 caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa gtagtgaaag
    31441 cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat aaagcgaaca
    31501 gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga acagaacaaa
    31561 tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc gaagcttatt
    31621 cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa gcagagaaaa
    31681 aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt atgcattatt
    31741 ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt tactgggaag
    31801 aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac gggacgttag
    31861 agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa cagagagtgt
    31921 tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca ttgatagaca
    31981 caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt ttgtctttct
    32041 tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg aactgccatc
    32101 cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg gataaaaaga
    32161 atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa atattggcat
    32221 caattacgtt ataaaacaca
//
LOCUS       DACEKQ010000023        32104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEKQ010000023 DACEKQ010000000
VERSION     DACEKQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.024"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1041
                     /locus_tag="IW671_000982"
     CDS             352..1041
                     /locus_tag="IW671_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0897982.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IW671_000983"
     CDS             1028..2236
                     /locus_tag="IW671_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0897983.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IW671_000984"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IW671_000984"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0897984.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4046..4741)
                     /locus_tag="IW671_000985"
     CDS             complement(4046..4741)
                     /locus_tag="IW671_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0897985.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4831..5931)
                     /gene="ychF"
                     /locus_tag="IW671_000986"
     CDS             complement(4831..5931)
                     /gene="ychF"
                     /locus_tag="IW671_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0897986.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(5940..6131)
                     /locus_tag="IW671_000987"
     CDS             complement(5940..6131)
                     /locus_tag="IW671_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0897987.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6152..7042)
                     /locus_tag="IW671_000988"
     CDS             complement(6152..7042)
                     /locus_tag="IW671_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0897988.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7029..7796)
                     /locus_tag="IW671_000989"
     CDS             complement(7029..7796)
                     /locus_tag="IW671_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0897989.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7808..8521)
                     /gene="rsmG"
                     /locus_tag="IW671_000990"
     CDS             complement(7808..8521)
                     /gene="rsmG"
                     /locus_tag="IW671_000990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0897990.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8753..10654)
                     /gene="mnmG"
                     /locus_tag="IW671_000991"
     CDS             complement(8753..10654)
                     /gene="mnmG"
                     /locus_tag="IW671_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0897991.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10671..12068)
                     /gene="mnmE"
                     /locus_tag="IW671_000992"
     CDS             complement(10671..12068)
                     /gene="mnmE"
                     /locus_tag="IW671_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0897992.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12412..12750
                     /locus_tag="IW671_000993"
     CDS             12412..12750
                     /locus_tag="IW671_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0897993.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(12918..13676)
                     /locus_tag="IW671_000994"
     CDS             complement(12918..13676)
                     /locus_tag="IW671_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0897994.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13694..14512)
                     /locus_tag="IW671_000995"
     CDS             complement(13694..14512)
                     /locus_tag="IW671_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0897995.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14509..14865)
                     /gene="rnpA"
                     /locus_tag="IW671_000996"
     CDS             complement(14509..14865)
                     /gene="rnpA"
                     /locus_tag="IW671_000996"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0897996.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(14983..15117)
                     /gene="rpmH"
                     /locus_tag="IW671_000997"
     CDS             complement(14983..15117)
                     /gene="rpmH"
                     /locus_tag="IW671_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0897997.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15640..16974
                     /gene="dnaA"
                     /locus_tag="IW671_000998"
     CDS             15640..16974
                     /gene="dnaA"
                     /locus_tag="IW671_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0897998.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17172..18302
                     /gene="dnaN"
                     /locus_tag="IW671_000999"
     CDS             17172..18302
                     /gene="dnaN"
                     /locus_tag="IW671_000999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0897999.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18531..18773
                     /gene="yaaA"
                     /locus_tag="IW671_001000"
     CDS             18531..18773
                     /gene="yaaA"
                     /locus_tag="IW671_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0898000.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18760..19884
                     /gene="recF"
                     /locus_tag="IW671_001001"
     CDS             18760..19884
                     /gene="recF"
                     /locus_tag="IW671_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0898001.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            19881..21827
                     /gene="gyrB"
                     /locus_tag="IW671_001002"
     CDS             19881..21827
                     /gene="gyrB"
                     /locus_tag="IW671_001002"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0898002.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21847..24318
                     /gene="gyrA"
                     /locus_tag="IW671_001003"
     CDS             21847..24318
                     /gene="gyrA"
                     /locus_tag="IW671_001003"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0898003.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24554..24853
                     /locus_tag="IW671_001004"
     CDS             24554..24853
                     /locus_tag="IW671_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0898004.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            24903..25415
                     /gene="ssb"
                     /locus_tag="IW671_001005"
     CDS             24903..25415
                     /gene="ssb"
                     /locus_tag="IW671_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0898005.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25441..25677
                     /gene="rpsR"
                     /locus_tag="IW671_001006"
     CDS             25441..25677
                     /gene="rpsR"
                     /locus_tag="IW671_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0898006.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25820..27793
                     /locus_tag="IW671_001007"
     CDS             25820..27793
                     /locus_tag="IW671_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0898007.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27799..28251
                     /gene="rplI"
                     /locus_tag="IW671_001008"
     CDS             27799..28251
                     /gene="rplI"
                     /locus_tag="IW671_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0898008.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28525..29892
                     /gene="dnaB"
                     /locus_tag="IW671_001009"
     CDS             28525..29892
                     /gene="dnaB"
                     /locus_tag="IW671_001009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0898009.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            30672..31964
                     /locus_tag="IW671_001010"
     CDS             30672..31964
                     /locus_tag="IW671_001010"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0898010.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9877 a         6200 c         6389 g         9638 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc
     3961 ttactattat ttaactatgg ataaacaaaa tgaggttctt tactacccct agatatctag
     4021 caaaatagct gatctttttg ctagtttatc ttcttgggac ggataaactt ccaataatcc
     4081 cgtatttttt cttcaagtag taacggattg ctaaagcagt tgccccgata atgataacta
     4141 ctactggatc taatactgga ttgataactg gtggcaatag tgttgtcgca gaaaacagta
     4201 cgacccaaag aagtgtcgtt ccacctagga taagcaatgt cttacccatt cctggtttct
     4261 tgcttcgatc tgctccggga tattcatatt gatagatgta tttgtacatc atataaaaga
     4321 cccagccacc gattgcagaa cctagaacga gtgtagaaaa gccataagct ggcgttgttc
     4381 cacgtgaaaa caatccggta atcgcaatca tgatacttaa tagaccaagc aacagcaacg
     4441 tattatctaa ccacatcaat aaaggggtgg atgaattttt tacttctgct ggcttattga
     4501 tgattgcttc cgttctttca gagactgtcc caaacaattg acgggctgtt cttccgcctt
     4561 tttgttctgc aactaaagta ggcagaatgt catgaagggc ttttactttt tcttcttcag
     4621 acaaattcgc tgcttctaaa gattttttca aatcaaaaat gtactgttga tttcttttgg
     4681 ttaattttgt ttctaaacta ttgttttctt ttacgattgc tcgtaattgt tctggttcca
     4741 ttatctaata gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa agacattcta
     4801 ccatattcta cagctagttt gatagccctt ttacacatta aagcggaata acattacgtc
     4861 gccgtcttgt acgacataat cttttccttc taatcgaaca cgtccagctt cttttgctgc
     4921 atgcatgttt ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac ggataaatcc
     4981 gcgttcgaaa tctgaatgga tgattcctgc tgcttgtggt gctttgattc cttttttgaa
     5041 tgtccaagca cgtacttctt gttccccagc agtaaagtaa gtagctaagc ctaacaaatc
     5101 ataagcagca cggatcaact gatccaaacc tgattcttcg attcctaaag cttctaaaaa
     5161 ttccgctttg tcttcttcat ctaattctgc gatttcttct tctgcacgag cactgaccac
     5221 gatcacttct gcattttctt ctgcagcaaa gttgcgcact tcttgtacat aatgattcgc
     5281 gtctgcatct gctacgtcat cttctgagac attggctaca taaagaattg gttttgtcgt
     5341 caataaaaat agtgatttaa cgattttttg ttcttcttca gtaaattcga tcgttcgggc
     5401 agattttcct tcttccagta caggtttgat tttatccagt acagctagtt ctgccactgc
     5461 atctttatct ttggttttag caacttttgc tacacgagta tagcgtttgt tgatagattc
     5521 taagtctgct aacactagtt ccaagttgat cgtatcaata tcttctagtg gatcaacacg
     5581 tccttctacg tgagtaatat tatcatcatc aaaacagcga acaacgtgac aaatagcgtc
     5641 tacttggcgg atatggctta agaattgatt tcccaaacct tctcctttac ttgcgccttt
     5701 tacgattcca gcaatatctg taaattcaaa agtcgtcgga acagtttttt tcggatgtac
     5761 aagttctgtt aagcgttgca aacgccagtc aggtacttct accatcccta cgttggggtc
     5821 gatcgtcgca aagggatagt ttgctgcttc tgctcctgct tttgtgatcg cattaaacaa
     5881 agttgatttt cctacgttgg gcaacccaac aattccagca gtcaatgcca taaatttatt
     5941 cactctttct ctgcattttt ttcaattact ttttttaatt tcttttcaaa atctcgacgg
     6001 gtcatcatga cgatatgtcc gcaattcata catctgattt tgatatctgc gcccattcga
     6061 atgacttccc agcgattcgt ctggcaagcg tgaggttttt tcatttcaac gatatcgtgc
     6121 aagtcgtaca taaaaatagc ctcctttttg tttattcttc atcaaattga atattcaaga
     6181 tatcaagaat tcgagtcaaa tctttttgag aaagatattc aatctcgatc ttacctttcc
     6241 catttttttc ttggatagct acacttgtac caaatttatc catcaaacga tcttcgcttt
     6301 ctcggatata atatggtttt tcctttgcta gccttgggat ttttttcttt tcttttccat
     6361 cattttgatt taactcagaa acaagctgtt ccagttggcg gacagttaaa ttttcagtta
     6421 ctgcacgatt ggctaatttc aagatcaatg ttttattttt taaacctaac agcgtacggg
     6481 cttgtcccat agataaacgc tgatcttgta ccatttcctt gacaagatcc ggtaaagaaa
     6541 gcaagcgcaa ataattggcg atataaggtc tgctctttcc taaacgacta gctacttctg
     6601 cttgtgttag tttcaggttt ttcatcagca tctcatatgc ttctgcttct tctaacggat
     6661 tcagatcttc tcgctgcaag ttttccagca ctgctacttg catcatcgct tcttcgtcaa
     6721 agtctctaac gatagcaggg atcgtttctt tttcagctag tttggacgct cgaaaacgtc
     6781 gctcccctgc aataatctca tatcctttta ctgcggaacg gcgtacgatg atgggttgaa
     6841 agacacctga ttgttcaata gaattagcta gttcctgcaa agatacttct tcaaatgttt
     6901 tacggggctg atacggattt ggtcgcagct cattcaatgg tagctggaca acggtatcat
     6961 cttttacatc caccgtctct aagttttcta aatcttgaaa cagtgcatcg attcctcggc
     7021 ctaatccttt acttttcttc acgacttacc acttcctttg ccaatgcttg gtagacttca
     7081 gctcctcgag aacgtggatc gtagtcgatg atcggttttc catgactagg tgcttctgat
     7141 aaacgaatat ttcttggaat gatcgtttcg taaacttttt cgcggaagta tttacgtact
     7201 tcttctacga cttcatttcc cagatttgtt cgtgcatcat acattgtcag taagactccc
     7261 tcgatctcta attctggatt aaagtgtttt tggactaaac gaaccgtatt cagtaattgg
     7321 cttaatcctt ccaatgcgta atactcacac tgaacaggta tcaaaatcga atcacttgct
     7381 gtaaatgaat tgattgtcag atggccaaga gaaggcgggc aatcgatcaa aatataatca
     7441 tactgactgc ttacttctgc taaagatcct tttaatcttg attctcttgc catcattgac
     7501 gtcaattcga tctctgctcc tgctaattgg agagtcgcag gcacaatact caaattttcg
     7561 tgttcagtga tcaaagttgc ctcatcaatt ggcaattcat tgactagaac atcatagatg
     7621 tctcttgtca catcaggttt tctgatcccg acaccactgg tagcatttcc ttgtgcatcc
     7681 atatccacta gcaatacttt tttgccaagg ctagcaagac aagcacctag attcaccgtc
     7741 gtcgttgttt tgccgacgcc acctttttgg ttcgcaacag atattatttg tgccatctct
     7801 tctcctccta tttgattggc tgcttattag gcagtccggg ctttcttggg tatttctttg
     7861 gggttggttt ctttttttga ataacgatga tatgccgttg atcagagaca ttcggcaagg
     7921 taaccgcctc ttctttgagg aatttaccac ctaataaagc aatcgcttgt tttgcttctg
     7981 tcaattcctc ttcacttttt gctgctttca atgcataaaa atagccttct tgtttagcca
     8041 agggcaaaca caattccgct aacacattca aacgagcaac cgcacgagca gtgacaaggt
     8101 caaatgatgc acgaaagtga ggattttgcc caaatgtttc tgcccggtca tgatataagg
     8161 aagcatcgat tcctaataat cgggttaact cagataagaa ggttattcgt ttattcagag
     8221 aatcaacgat cgttatctgc agttctggaa aggcaatttt taaaggtatg ctaggaaacc
     8281 ctgcccccga accaacatcg cataaagata aaggttgcgt aaagtctact tcaaatgcta
     8341 atgtaagtga atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta atagctgtaa
     8401 ggttcatctt ttcgttccat tcgactaatg tccgataata aatagcgaat tgattcattt
     8461 gatgatcggt aagctctatc cctttttctg ccaaggattg gcgaaattct tctggtatca
     8521 taaatattcc tttcattgtg aaacagcaat tgtttcacag attctctccc tactttaacg
     8581 caaaatctct agacatgcaa tccttccata tggattctta aaaatttgaa aagtaagaaa
     8641 agccgataga ctttttcttt ctagtatgac tcataaaata tacacagaag agagaactag
     8701 ctttgagcaa tttctgcaaa aactagttct ctcttctatt tattattacg tattacgctg
     8761 gttcactgac tttcgcaatc ttcccttgtt cgatatagac cattaggata cttaggtctg
     8821 cagggtttac gccactgatt cggctagctt gggcaattgt ttctggttgg attttctgca
     8881 atttttgttt tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg ttttctggaa
     8941 tctttttcgc ttccatgcgt tttaattttt ctactttttc catggctttt ttgatgtagc
     9001 cttcatattt gatttggatt tctacttgtt cgatttcttt tcgtgtcaat tcttcatttt
     9061 ctggaataaa ccgtagaagt tctgcataag taatctcagg acgtcttaaa aagtcgcttg
     9121 ctagtacacc atcttttaat ggtgccgcat taatcgttgc taaaaagtct tggacttctt
     9181 ctgttggttt gattcgtaca ttcatcaaac gtttgatttc ttgttcaact gcttcttttt
     9241 tcttcaaata attcttgtat tgatcttctt ttactaagcc tacacggtga cccatttctg
     9301 ttaatcgcaa atctgcatta tcatgacgta aaagcagacg gtattcagca cgtgaagtca
     9361 gcaaacgata aggttcgttc gttccttttg ttaccaaatc atcgatcatg acaccgatgt
     9421 aaccgtcact tcgtttgatc actaaaggtt cttttccctg aactttcaaa gctgcattga
     9481 taccggcaat cagcccttgt ccagccgctt cttcataacc gcttgtacca tttgtttgtc
     9541 cagctgtata aagattttca acgactttag tttctaatgt cggacggagc tggtgtggct
     9601 ctactacgtc atattcgata gcatatcctg tacgcatcat ttctactttt tctaatccag
     9661 caatagaatg aagcatgttg acttgaacat cctctggtaa agaagtagat aaaccttgaa
     9721 catagacttc ttccgtattt aagccttctg gttccaaaaa taattgatga cgtggtttgt
     9781 ctgcaaaacg cacgatctta tcttcgatgg atggacaata gcgagcgcct accccttcaa
     9841 cgatccctgt aaacattggg gcacgatgta agttgtctcg aataatttgg tgagtcgtct
     9901 cacctgtata ggtcagccag caagattcct gatctagttt ataggcacta tctggtgtgc
     9961 tgaagctaaa atgattcggc tctttgtcac caggctgctc ttctgtaaca gaatagtcga
    10021 tcgtacttga ttttactcga ggcggtgttc ctgttttgaa ccgttcgata gcaaagccaa
    10081 gttcttttag atgattcgct agtccaatag aaggttgaga gttattcgga cctgatgaat
    10141 atttcaattc accgataatg atttctccgc gaagtgctgt tccggctgta ataacaactg
    10201 ctttggcccg gtagctcgct cctgtggacg tcactactcc atgacaaaca ccatcttcga
    10261 cgatcagttc ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt tcgatcgtat
    10321 gtttcatttc tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct cggacagctg
    10381 ggccttttcc tgtgttcaac atacgcattt ggatataggt tttatcgata ttcttaccca
    10441 tttctccgcc taacgcatcg atttctcgaa cgacaacgcc tttagctggt ccgcctacag
    10501 aaggattaca tggcatgaat gctaccatgt ccaaatttat tgtcagtaat aatgtttttg
    10561 atcccatacg tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga
    10621 tcacgtcata cgcatctgct tcataatgtt ccaatcgttt gttcctcctt ttattttcct
    10681 aaacaaaatt gactgaataa ttgagtgatt agctcatctt gtacactgtc gccgacaact
    10741 tctcctagat agtcccagca acgcgtcata tctatttgaa cgagatccac aggcattcct
    10801 gaatcaatac ctgcaatgac ttcttcaagt gagagggaag ctttttctaa caacgcgata
    10861 tggcgggtat tagaaacata agtagcgtct ctttctcctg tttctccgct gaagaataaa
    10921 tcagaaatag ctgattccaa acgatctagt ccgtcgtttt tagctacgga aattgaaaaa
    10981 actggttcat tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct
    11041 gttttattta gaagaatgat ccgtttcaat ccacttgtcg cttccaataa ttgttcatct
    11101 tctgctgtca gtggttcgct ttggtttaga acaagtaaga tcaagtctgc ttctgctaat
    11161 gcttttcggc ttctttcgac cccgatccgt tccaccacat cttctgtttc ccgaattcca
    11221 gctgtatcga ttagcttgag cggtaccccc cgaacattga catactcttc gattacatct
    11281 cgagtcgttc ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa atggtttaac
    11341 aaacttgatt tgccgacatt tggccggccg atgattgccg tacttaaccc ttcccgcaga
    11401 accttccctt gcttggatgt agcaagtagc gcttggattc tttgttggat catctgcgct
    11461 ttttcaagca ataacttcgt agtcaattct tcgacatcat catattctgg ataatcaata
    11521 tttacttcta cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca
    11581 gacagattgc cgtctaactg attaagagct agtcccattg ctttgtctgt ttttgcccga
    11641 atcaaatcca tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt
    11701 tttgtgaatt cgcctggctc agctaatctt gcaccttctc ttaacaatag ctgcaaaatt
    11761 tgattgacta caacgatccc accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat
    11821 gtctttggtg ctctcatcac ggagaccatg acttcatcga caagctgatt gctcttggga
    11881 tcaacgatat gtccataatg gatcgtatga cttggtactt ctgataatcg tttgtttcca
    11941 gattgataaa ctttatctgc tagttgcaca gcttgatcgc cacttaaacg aacgatactg
    12001 attgcacctt ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt
    12061 tcagccattt tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc actccaccct
    12121 ttatgcacaa taaacggtga acttcgcact ttgactgctt gttcttgatt aaattctggt
    12181 catagattat cacacttgct ataaggaatc aagtgtttaa aacaattaga tgaagttgag
    12241 cctgttctgc ttccattacc ttatatttaa ttgtccttgg atgtctcttt gcgttctttg
    12301 gtttattcga agagaagata ctgtataaac aaggctttat ctttcttaaa aaaaacggta
    12361 cactaaatgg tagcgctatc taaactatac acttttgaaa ggagaaaata tgtgagcccg
    12421 ttcagatctg ctgcattgga agaagaaagt gaccctcgga tcaacgatgt cgttttatct
    12481 acagggaata tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt ggaatgctta
    12541 gaagctgatt tgtttgaccc atcttttccc aaagatgacc ttttcttaac aacgaagagg
    12601 aaattaaaga cgaaggtact tgaatatggt gcgaatgcag taatcaactg tcattttgag
    12661 tatcagcaaa aaaaacgcga cggaaagtat tttctggaac tttttgctta tggaacagtg
    12721 atccaattta tccaaacaac gattggttaa atgaaacaga caaaaaaagc ggatcaaaac
    12781 tgaagacagt ttcgatccgc ttttttctat caaagttagt gactacttca tcgtatactc
    12841 tgataagaat gatttctagt aaaatatact tggtttgaaa aactttgtca acgtgattta
    12901 ttcaaaaagt aatggaatca ataatatttt ttagcaggtt ctactactaa ataacgataa
    12961 ggttcctcac cttccgaatg tgtttttaca tgttcatctt tgcttaatgc agcatggatt
    13021 tgtttacgtt caaatgcagg cattggttct aagaaaacag gctgccctgt ccgttttact
    13081 ttttcagccg tgcgatcagc taaacgctga atgatctctt ggcgcttttc acggtaattg
    13141 cctacattga ctactacaga cagcttattt cttgctaaac gatggatgaa gacttgtgcc
    13201 agatattgca atgcattcag tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt
    13261 gtttctaaat ggaaaacaac catattattt gattctcttt ccattttgac aagagctgga
    13321 gcatcaagct gttttgaaat ttcagtaaga taaactgcca attccgttaa agctgcttca
    13381 tcacctagct cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg
    13441 atctcagctg ttggttcttc ttccaaaatt tcttctacag tctgttctac tgtttcagaa
    13501 actttttcgc tgatacttgg ctggatcgaa agccgtgcat tctttcgacc cattcctagg
    13561 aaaccttttt ttccttcttc cagtatttcg atttcgactt ggtctttggc caaacccaaa
    13621 tcagccagac ctagttggac agcttcgtct attgttgcag cttcataaat cggcattagc
    13681 attacctccc ctattatttc cgttttttct taggattcat tgctttctta agagcacgtt
    13741 ctctttcgcg gattctccgt gcttcttctt cacgctcttt tcgaattttg aaagggttgt
    13801 tgatcagcag cgtttgtaca acttggaagg cattggaaac tacccagtat aatgaaagac
    13861 cgctggctaa attgacaccc atcactaaaa tcattacagg catcacgaaa ttcatgatct
    13921 tcattgatgc attcgattca atttgactca tgctagataa atacgtactt gcaaaagtaa
    13981 agattgctgc taaaatcggc aaaataaatg ttggatccgc gttccctaag ttcaaccata
    14041 agaaatgccc ttgtttcaac tctggtacac gtgaaataga ttgccataac gccatcaaga
    14101 ttggcatttg tactaataaa ggaagacaac cagcatatgg attgacgcca ttttctgcat
    14161 ataaacgctg ctgctcttct cttaacttac tttgtgtttc cggatctctt gaactatatt
    14221 gttgctgcag tgcttttaat ttcggttgta attcttgcgt tttacgcatg ctctttgttt
    14281 ggaaatgcat caacggcaat aggatcacgc ggatgatcag tgtaaataaa atgatcccta
    14341 tccctgcatt accaaatgac aacgccttga tcgcctcagc aaagtaataa acaatataac
    14401 gatcccaaat tcctgtactc tgtgcgctga cttccccagt tccgcaaccg gataagacga
    14461 tgaccaaact aacgataccg gccatcaaaa gtaaacgttt gtatttcttc acttactctt
    14521 ttcctctctg tcgattattt ttgcaagttt taagacgtgg actaaattgg agcgaacctc
    14581 atcataagtc aaccccttca cacttggtct agcaatcaca ataaaatcca actccggatc
    14641 aatttcttct tttatagaat aaactgctgc tcggattttt cgtttgactt cattgcggtt
    14701 taccgcgttg ccgacctttt ttcctacaga taaaccaact cgaaaatgag gctgtcccgg
    14761 tttttccaaa cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg tatggaacac
    14821 ggcttgaaac tctcgttctt tttttactcg atacgctttt ctcattagtt ttctttcctt
    14881 ttctatctct cacttacgct cataatcata ccataattac gattgtcctt tcatcatttc
    14941 cctaaaaaag agtataaaaa aaaccactga aaccccagtg gcctaagctg aaagaacttt
    15001 tcttcctttg cgacgacggc tagctaacac acgacgtcca tttttagtac tcatacgttt
    15061 acggaatccg tgaacttttt gacgtttacg tttatttggt tggtatgttc ttttcatata
    15121 ttccacctcc aaaatgttct tatctatttt ataaacacag acatactttg atagtataac
    15181 gacaaagtag tgattttgtc aatataaaat agaagatcga ttgttaaaac ttatccacaa
    15241 gattattttt ttctatggga atcctatgtg gaaaaagtta tgcacaggga ttatccacag
    15301 ataataaaag gctggtttta cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag
    15361 gcgatttcct gtgtattccg tctcctaact attggtatat ctgtattttt tatccacaga
    15421 cgcaagagga aatctattgg tttttacgag ttttccacct gtgcattagt ggtgtggaaa
    15481 actttttttg acaactgtgt ttttctcttt tttctttttg tcgattgtgt ggataattgt
    15541 aattaaaaaa tttttttctt cggtttctgt ggaaaactat tgaaaaaaat gctaaattag
    15601 tactagttat tcacttttta atgaaggagg aggccttata tggtatccct cgatgcttta
    15661 tggaatgaat taaaagcaac ataccaaaag gatttatcgc ctgctagcta taatacatgg
    15721 atcgaaacag cgaaccctcg aacccttgat caaaaccagc tcgttgttga ggttcctagc
    15781 aagattcata aagaatattg ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat
    15841 atgttatcag gaaatgagat tatccctcgc tttattactg gcgaagaagc agagcaagaa
    15901 gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg
    15961 ctgaacccta aatatacctt tgatacgttt gtcattggta aaggaaacca gatggctcat
    16021 gcagcagcgc tcgttgtagc agaagatcca ggttctattt ataacccgct cttcttttat
    16081 ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa ttggtcatca aatgctgcaa
    16141 agtcagccta atgccaaagt aaaatatgta agcagtgaaa cttttgctaa tgatttcatc
    16201 aattcgatcc aaaacaaaac agcagaagag ttccgtcaag aatatcgaaa tgttgattta
    16261 ctgctagtgg atgacattca attttttgca gaaaaagaag ctacgcaaga agagtttttc
    16321 catactttca atgccttgta caatgaaggg aaacaaatcg tactaaccag tgatcggttg
    16381 ccaaacgaga ttccgaaact gcaagaacgc cttgtttctc gctttgcatg ggggctgtct
    16441 gttgatatta caccgccgga tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg
    16501 gctgaacgtc tagagatccc agatgacaca ctaagctaca tcgcaggaca aattgattct
    16561 aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt
    16621 gaagatattt cgactagtct agctgcagac gcactgaaaa cgttaaaatc aggaaaagga
    16681 catcctcaat tgtcaatcct gcagatccaa gaagaagtgg caaagtatta tcacatccaa
    16741 ttgaaagatc tcaaaggaaa gaaacgcgtc aaatctattg ttgttcctag acaaatcgct
    16801 atgtatttgg cacgtgagct cacagataat tctttaccga aaatcggagc tgaattcggt
    16861 ggcaaggatc atacaacggt tatccatgct catgaaaaaa tccagcagct gatggattct
    16921 agtgtcagta tgcaaaatga agtatcagaa atcaaaaact ttttactaaa ttaatttgtc
    16981 gattctatca actgaaaata gcatgtggat aaaagaaaaa aacgttcaaa agttatacac
    17041 actttttcca cagctgtttt ttcgtttctc tcttacgttt tttgagtttt ccacagaatc
    17101 aacatgccct attactttta ttattctttt taactaataa taaataaaca aacattcaaa
    17161 ggagatacat catgaaagtt actttaaacc gagctagctt tatgcaggaa ttgcaaactg
    17221 ttcaacgagc tatttcaagc aaaaccacga tccctatttt gacaggtgta aaaatcacac
    17281 tgacacaaga aggtttgact ttgacgggga gcaacgctga tatatcaatt gaaacttttt
    17341 tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc tactggttcc attgttttac
    17401 aagcacgttt ctttagcgaa atcattcgga gacttcctga agaaacattt actttagaag
    17461 ttttagaaaa taaacaagta gcgatcactt ctggaaaagc gaattttatc gtaaatggat
    17521 tagatgcaga taactatcct catcttcctg ttgtcgaaag ccataaccag atgaaattac
    17581 ctgtacacgt attgactaaa ctaatcaacg aaacagtttt tgctgtctca caacatgaga
    17641 gtcgtccaat cttgacaggt gtgcatttca ttttatctga taattcttta ttagctgtag
    17701 ctactgattc tcaccgtcta agtcaacgcg tgattccagt agaacaagcg gctgatcatt
    17761 ttgatattgt tattcctgga aaaagtttga tcgaattatc tcgctcatta acaaatgaag
    17821 aagaaatcgt cgaaatcagc attatggaaa accaagtgtt gttcaaaaca gaaacgatgt
    17881 atttctattc ccgtttgcta gaaggaaact atccagatac caatcgtttg attccttcaa
    17941 gttttaatac agaggttgaa ttttctgttc ctagcttttt agcggcgatc gaacgggctt
    18001 ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg
    18061 ttgttttata cggaaattcg ccagaaatcg ggaaagtaga agaaagcttg agttatacag
    18121 caagcagtgg cgatccatta gatatttctt tcaatccgga ttatatgaaa gcagcgttgc
    18181 gtgcatttgg agatatgagc atcaaagtga aatttatctc tgctattcgt ccatttacat
    18241 tggaaccaac ggaagatggc gttcaattta ttcagctgat tactcctgta cgtacaaatt
    18301 aacaatgatt ttcaatgttc ttgaaaattt aattgaaaag aaatagaaaa aataaaattt
    18361 taaacagaag gccctaaaat ctcttttaag gctttctgtt tatttttgtt taaatcacct
    18421 tttttagatt aaatcgtctt atatggaaaa atatatggat aaatttgttt tattgcgaaa
    18481 aaaaaggtat aatagagtat acttttaata atgaaaatga gggatgggca ttgaaacaac
    18541 aagtcattct aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta
    18601 tcggtagtgg cggacaagca aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg
    18661 aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa gttccagaag
    18721 taggaacttt ttttatggta aaaaaaggaa acgacgataa tgaggctgaa taagctgtat
    18781 ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat tttccaagaa cctcgtcatc
    18841 tttttaggag aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta cgtattagct
    18901 atgacgcgaa gtcatcggac aagtagtgag caggaattga ttggctggac tgacgatcag
    18961 gcgatgattc aaggagagat cacaaaaggt tcatccactc ttccattaga aatcttatta
    19021 tctaaaaaag gccgaaaaac caagatcaac catatcgaac agaaaaagtt aagtacttac
    19081 gtgggacaaa tgaatgtcat tttatttgca ccagaagatc tttctttagt aaaaggcagc
    19141 ccgcagacca ggcggaagtt tttagatatg gaattaggcc agatcaatcc aatctatctt
    19201 tatgatcttg tccaatacca agcaattttg aaacaaagaa accagtactt gaagcaattg
    19261 aatgaaaaaa aacaaactga tttgctttat ttggatattt tgtccgaaca actagcagcg
    19321 tttggcagca aagtattgaa agcaagagca cagttcgtca agcgattgga gttttgggct
    19381 aattctttgc atcagcaaat cacccatcaa aaagagcagc tggaaatcga gtatctcacc
    19441 gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc aagaacaatt tctcgcactt
    19501 ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa cgacagttgg tcctcatcgt
    19561 gacgatcttt ccttttttat taatcagaaa aatgtgcaga cttatggatc gcaaggacag
    19621 caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg atttgattaa agaagagaca
    19681 ggggaatatc caatcctttt gttagatgat gtgatgagcg agctggatga taatcgtcaa
    19741 ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt
    19801 gaccatgtaa aaaataaaat gactgttgaa cctgagattt tttatgttca acaagggaaa
    19861 ctaaaaggag aagaaactga atgacagaag aaagaagttt agtagaacgc gctaaagagt
    19921 atgatgccag tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa cgacctggca
    19981 tgtatatcgg ttcgactagt tctgagggtc tgcatcacct tgtttgggaa attgtcgata
    20041 attcgatcga tgaagtatta gcaggttttg caacgaaaat ccatgtgatt attgaaaaag
    20101 acaacagtat caccgttatt gacgacggtc gggggatacc tgtagatatc caggcaaaaa
    20161 ctggacgtcc tgcagttgaa acggtcttta ctgttttaca tgctggagga aaattcggcg
    20221 gtggtgggta caaagtttct ggtggacttc acggagtagg atcttctgtt gttaacgcgc
    20281 tttctacaca gcttgatgtc aaagtataca aagaaggaaa tgtgtactat caagaatatc
    20341 gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca aacggatcgc catggaacaa
    20401 cggttcactt tacgccagac cctgaaattt ttacagagac aactgagttt gatttttcaa
    20461 aattggctac ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt atatcgattg
    20521 aagacaaacg agaagaagaa ccagtcatca atgaatacca ttatgacggc gggattaaaa
    20581 gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg
    20641 aaggtgaaca gcaagatatc gcagttgaag tctctatgca atatacagac ggctaccatt
    20701 ccaatatcat gagttttgcc aacaatatcc atacgtatga aggcggaaca catgaatcag
    20761 gattcaagac tgctttaact cgtgtgataa atgattatgc ccgaaaacaa aagctgatga
    20821 aagaaaatga cgacaacttg accggtgaag acgtgcgaga aggattgaca gctgttattt
    20881 ctatcaagca tcctgaccct caattcgaag gacaaacaaa aacaaaattg ggcaattccg
    20941 aagtacggac agtcactgat cggttattta gcgaacattt catgaaattc ttgctggaaa
    21001 acccaagtgt gggccgtcaa attgttgaaa aaggattact agcttccaga gctcgtctag
    21061 cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt agtaatctgc
    21121 caggaaaatt agcggactgc tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg
    21181 aaggagattc ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt caagccatct
    21241 tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag catggacaag atcttggcaa
    21301 atgaagagat ccggtcttta tttactgcta tgggtacagg gtttggagaa gattttgatg
    21361 tatcgaaagc tcgctaccac aaattagtta tcatgactga tgccgatgtc gacggcgcgc
    21421 atatccgtac attgctgctg actctttttt atcgttacat gcgtcctatt gttgaagctg
    21481 gatacgtcta tattgctcag ccacctttat acggggtaaa acaagggaag aatattacgt
    21541 atgtacaacc aggaaaaaat gcagaagaag agttgaaaca agtcgttgct tcacttcctg
    21601 cctcaccaaa accaagcgtt cagcgttata aaggacttgg agaaatggat gatcatcaac
    21661 tatgggaaac aactatggac ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg
    21721 ctattgcagc agatcagata tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg
    21781 cattcatcga ggaaaatgca cactatgtga agaacttaga tatttaatca tgaaggagga
    21841 aaaagaatga gtgaagaaat cagagaaaac atccatgatg tcaatctaac cagcgaaatg
    21901 aaagactcct tcatcgatta tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc
    21961 agagatggtc tgaaacccgt tcatcgccgt attttatacg gcatgaacga attaggtgta
    22021 acaccggata aagcccataa aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac
    22081 catcctcatg gagactatgc gatctatgag tcaatggtac ggatggcaca gccattcagc
    22141 taccgttata tgctagtgga tggtcatgga aactttggtt ctgttgacgg tgacggtgcc
    22201 gccgctatgc gttataccga agcacggatg agcaaaatcg ctacagaaat gctgcgtgat
    22261 atcaataaga acactgttga tttccaaagc aactatgatg atacagaaaa agaaccagtt
    22321 gtactacctg ctcgcttccc gaatctttta gtgaacggaa caacggggat cgccgtaggt
    22381 atggccacga atatcccacc tcataacttg tctgaggtag tagcggctat cgatttgctg
    22441 atggataatc ctgatgtgac gaccaatgaa ttgatggaag tattacctgg accagatttt
    22501 ccaacaggtg gtttggttat gggaaaatca ggaatccgaa gagcgtatga aacaggtaag
    22561 ggttctatta ccgtacgtgc aaaagttgag attactgaga tgccaaatgg taaagaacgg
    22621 atcatcgtga cagaattgcc ttatatggtc aataaagcaa aattgatcga acggatttct
    22681 gaactgcaca gagacaagcg tatcgaaggg atcacagatt tgcgggacga atcttcccgt
    22741 gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca gcgcatctgt tatcttgaat
    22801 aacttatata aattgacatc cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa
    22861 aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg aaaactatat cgaacatcag
    22921 cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa aagcagaagc aagggcacac
    22981 attttagaag gtttgcgtat tgctttggat catatcgatg aaatcattgc gattatccgc
    23041 gggtctaaat cagatgacga agcaaaagcg acgatgatcg aacggttcga tttatccgat
    23101 cgccagtctc aagctatttt agatatgcgt ttacgccgat tgaccggttt ggaacgggaa
    23161 aaaatcgaaa atgaatacca agaactctta aaactgattg aagatttaac tgacattctg
    23221 gcaagaccag aacgtgttac agaaatcatc aagaatgaat tagcagactt gaatcaacga
    23281 tttggtgata aacgacgcac agaattacta gtcggtgagg tcttaagtct ggaagatgaa
    23341 gatttaatcg aagaagaaga aatcgttatt acattgacaa acaatggtta catcaaacgt
    23401 ttggcaaata atgaattcag agctcaacga aggggtggac gtggtgtcca aggaatgggt
    23461 atccacaatg acgacttcgt caaaaatctg gtttcttgtt ctacccatga tacattgtta
    23521 ttctttacga ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc agaatacggg
    23581 cgcacagcca aaggaatacc aattatcaat ttactaggta tcgattcgag tgaaagcatc
    23641 caggcaatca ttgcggtgac gggagaagca gaagaaggcc actatctctt ctttacgacg
    23701 cgtcaaggta cagtaaaacg tacttctgtc actgcttttg ctaacatccg aagtaatggt
    23761 ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca atgtgctact gacagatggt
    23821 caatctaata tcattattgg aacacataac ggctactctg tcactttcaa agaagaagca
    23881 gtccgcgata tgggacgtac agcttccggg gttagaggaa tacgcctgcg tgaggaagat
    23941 tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag tattagtgat cacagaaaaa
    24001 ggttacggaa aacggacaaa agtatcggaa tatccagtga aaggccgcgg aggtaaaggg
    24061 atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag ctggattgac cactgtttct
    24121 ggagatgaag acatcatgct gattacagat aaaggtgtca ttatccgttt caacgtatcg
    24181 actgtttctc aaactggacg ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat
    24241 acgaaagttg tcacgatggc agcagtcgaa ccagaagctg cagaacaaac agttgaagaa
    24301 caaggatcag aagaatagcc atcaagacta attgtgtaag ctagcaagaa tgaccgatta
    24361 aaaaatctgg ttattcctgc tggctttttt gattagttga atttctctta aaatattcga
    24421 aactgttcat tctgtattgc ttttgaccaa aatagagtgt ataatcagag cttgtgagta
    24481 gtctaattaa ttagataact ctccttgctc ttaggaacct aagagccgaa agtccacagg
    24541 gaggtgaata tcaatggaaa acacgaaata tgaaatcatg tacattattc gtccaaacat
    24601 tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat gcaatcttga aagataacgg
    24661 agcagaagtt atcgaatcaa aagattggga aaaacgccgt ttcgcttatg aaatgaacgg
    24721 ttaccgtgaa ggtatctatc acatcgttaa agtatcttct ccatcatcag caaatgctgt
    24781 caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa
    24841 agaagaagct taattgttgt ttcatgtgaa acaatagaaa acgatgagag gagatgacta
    24901 ttttgattaa taatgttgta ttagttggac gtctgacgaa agatccagat ttgcgttaca
    24961 cagcaagtgg tacggcagtc gcaacattta ctttggctgt gaatcgtaat tttacaaacc
    25021 aaagcggcaa tcgcgaggct gattttatca actgtgtgat ttggcgcaaa tcagcagaaa
    25081 ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt tactggacgt atccaaactc
    25141 gttcttatga taatcagcaa ggacaacgtg tatacgtgac agaagtagta gctgacaact
    25201 tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc ggattccaaa
    25261 gttctggtca atcagctggc ggatttggcg gaaacaataa ctctaatcaa acatctcaat
    25321 catccaacgg tatgcctgat ttcgaccgtg atacttctga cccattcggc tcaagttcaa
    25381 cgatcgacat ttcagacgat gatttaccat tctaaacgaa tttgatagga gggaaataga
    25441 atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag tagactacat tgctgctaac
    25501 catattgaat atatcgatta taaagatatc gaattactaa aaagatttat ttcagaacgc
    25561 ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg taaaattact
    25621 gttgcaatca aacgcgctcg tatcatgggc ttgctacctt tcgttagcga agactaataa
    25681 atcctaggaa ttgaatgact gatccttttg atcagctatt caattccttt ttttctgtaa
    25741 aaataacctc tattcgttta gtcgctcgaa tgacgaaagt tatggtaaaa tcaaattagt
    25801 catatatggg aggaaaatta tggaaaataa aaacaatcat ttatccccga aacctctctt
    25861 ccttatcctg tttatattaa tcgaagtatt ggtagtctta attttaccat ttcgttttgt
    25921 tagcgcttcg gtcattctat tgatgaattt atggggtgtt cgctattggt tgaaaatgaa
    25981 acgttggctt attttaaata aacgtgaaaa aatccagcag gcttcgactg tagctgaaac
    26041 caatctagct tatgtctcag aagtcatgcc tgtaggagtg attgcttatc agccatcaag
    26101 ccaaaaaatc gaatggctca atccattagc attgaaaatc aaagatcaaa gtggattaac
    26161 agaggaagaa ttgctagata ctttttttac tgcaaaagaa aaaaagaaaa accaagtcta
    26221 tctagcagat aaaacatttc agtttgatat ggatacagta aagggtgttg tttattttaa
    26281 tgatattacc caaaaccagt tgttaaaact ccatcagacg aacagtcagc cggtgattgg
    26341 tattttatct attgataact atgcagatgc cattgataaa ttggatgaca aagagatttc
    26401 ttatttgaac tctttcgtca ctacactagt ttctgactgg atgaatgaat accatgtctt
    26461 tttcaaaaga atcaacgcag aacggttttt cttcgtagca cagatgatcg atctgaaaaa
    26521 gatgatggat aaaaaatttg atttgctgga tcgctttcga gcagaagctg aaaaacagag
    26581 ctcgcctatc acccttagta tggggattgc ttatggctca agaaccctcg atgaaatcgg
    26641 tggagaagct cagaataatt tagatgttgc tttagtccgt ggaggcgacc aagttgtcgt
    26701 gaaagaggta aaagaaggag caaaacctgt ttattatggg gggaaatctg ctaagaatgt
    26761 caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct ttgcgtcgga tttttgatga
    26821 aacaggcgat gtgtttatca tggggcacaa gtttccggat atggatgctt taggctctgc
    26881 ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa gcttacatcg ttatcaacga
    26941 gaacgagatc attccagatg tagaacggtg tcttgaagaa atccataagc atcctgaatt
    27001 agaaatgcaa ctaatctctc cagaacgtgc catagagctg aaaaaagaag aggacctcct
    27061 cgtgatggtg gattaccata aaccttctct atcgatctct cagggcctat atgagcaatt
    27121 tgataagatt gtgatcattg atcaccatcg tcgtggggaa gagtttccaa gtaaacctct
    27181 gctgacttat atcgaatctt cagcttcttc agcttcagag ctagtttctg agctgatcca
    27241 ataccaaagc agcaaaacaa atcgcttaga tcgttttgag gccacattat tgcttgcagg
    27301 gatggttgta gatacaaata gtttcaatgt ccgtacaact gcacgaactt ttgacgttgc
    27361 cagctattta aggtcttgtg gagcagacgc atccttagta caatatttat tgagctcgga
    27421 tttagcgaca tacttggaga tcagcaattt gattgctgga agcgactatg tgacttctga
    27481 tattgttgtg gcggcaggga cagacgaaaa agagtataat agtgttatag cagcaaaaac
    27541 agcagataca ttgctttcga tgatcaatat caatgcagct ttcgtcatta caaagcggac
    27601 agacggcttg attgggatca gtgccagaag taccggcacg atcaacgtcc aaatcatcat
    27661 ggaatcctta ggtggcggag gacacttcac aaacgcagct actcaactgg aaaatacgac
    27721 agttgaggaa acaaaacaga agctatttgc tgcaatcaaa gaaaatatga atcaaatata
    27781 tgataaggag taatccaaat gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa
    27841 aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa attatctaat caaaaataac
    27901 ttggcaaaag aagcaacgaa aagcagtatc agtcagttag caggacaaaa gaaagcacaa
    27961 gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa
    28021 gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag acagccgttt gtttggttcg
    28081 atcccttcaa aacaaattgc tgaagggtta aacaaacaat acaaaatcaa attagataaa
    28141 cgaaaaatcg aattagaaaa tccgatccgt tcactaggct acacaaacgt acctgttaag
    28201 cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg tacaagaata acaaatcttg
    28261 atagcgatgt tcagctggta agtataacca gaaggaaaaa agacaaaccg aaatttatct
    28321 gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt
    28381 ttcttttttg ggaacagcaa aatagcataa agaaaacgag tataataaaa gagaactatt
    28441 tttttattac actttcggta cgaatgatga cttttctctc attttcttgt aaaatgaagc
    28501 aattgaagac aaggagcgat acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa
    28561 aatattgaag ctgaacaagc ggttttaggc tctgtttttc ttgacgcaga aacgatcatt
    28621 gatgccatgg agtacataga gccaaaagac ttttatcgtc gtggccatca aattattttt
    28681 gagacaatga tcgagttaaa tgatcgtaat gaagcgattg acgtggttac tgtcaaagac
    28741 cggctggaac aagccaattt gttagaagat gccggtggtt taagttatct ctcagacttg
    28801 gcattagcag ttccgacagc agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa
    28861 tcattgctgc gtacattgat ccagacagca acaggaatcg ttacgaaagg attcgagcaa
    28921 ggagaagaag ttcaaacaat tttagatgat gccgaacgca gtatcttgga ggtttcggaa
    28981 aaaagaaatc gaagcggctt tttatctatt gctgaagtac tgaactccac gattgaaaat
    29041 atcgatcgac tagctcaaaa caatgaagaa atcacaggtc taccaactgg ctatcaaggt
    29101 ctggataaaa tgacagcagg tttccagccc gaagaactaa tcattcttgc tgcacgtcca
    29161 gcggtaggta aaacagcgtt tgcattgaat attgctcaaa atgtaggaac aaaaacagat
    29221 cgttctgttg cgatatttag tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt
    29281 tgtgcagaag gttcgattga ggccagccat ttacgtacag gtcagctttc ggaagaagaa
    29341 tggtcgaatc tgatcgttgc gatggggagt ctttctcgag caaatatttt tatcgatgat
    29401 acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa
    29461 ggcaatctag gcttgatatt gatcgattat ttgcagttga ttgaaggtaa cggtaaagag
    29521 aaccgtcaac aagaagtatc agatatctct cgccaactga aaaaactggc gaaagagctg
    29581 aaagtacctg tcattgctct ttcacagtta tctcgtggag tagaacagcg acaagacaaa
    29641 cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta
    29701 gccttcttgt atcgagatga ttattatgaa agagaaggcg gagatgacga tgatggaaac
    29761 gagccaaaaa atgacaacgt catcgaagtc atcatcgaga agaaccgaag cggtgcacga
    29821 ggaacagttg aactactgtt tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga
    29881 gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga
    29941 tgcaaggagt aaccgcagga acgactagag tacttaattt aaacagatct ccaaagggat
    30001 tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgacc
    30061 cttggtatta gctcagcaaa cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta
    30121 atactcaggt caaaacgctg atcttacgac ctcttttcct taaacggtgt tctcctgcca
    30181 actcctcggt cacaagtcac aatatcatca aacatgagaa gcagtttttt gaaattgctc
    30241 cttgtgactc agagttaaac ggcaggctca caacctcttt cttaaacggt gttcaaaagc
    30301 ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg agaagctatt tttgtttggc
    30361 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaaa
    30421 agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat ttgagaagca attttcgtac
    30481 attccttctt atttcttgaa gctgaccact tctgttacaa cctctttttt aataaccatt
    30541 ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa taaaaataaa aatcattcga
    30601 ttttatttga aatatgtatc tattattgat acaatggttc aggattaaat aatatagaac
    30661 gaggtgttca catgtcatct gtagtagtag taggtacgca atggggcgat gaagggaaag
    30721 gaaaaatcac agactttctc agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg
    30781 acaatgcagg gcacacaatt aagtttaacg gtgtgactta taaactgcat ttgattcctt
    30841 ctgggatttt ttataaagac aagatcagtg tgatcggaaa cggcgttgta gtcaatccta
    30901 aatcattggt taaagaatta gcttatttac atgaaaaagg tgtagaaaca agtaatttgc
    30961 gtatttctga ccgcgcgcac gtaatcttgc cttatcatat caaattggac caattacaag
    31021 aagatgctaa gggcgaaaat aagattggta caacgattaa aggaatcgga cctgcctata
    31081 tggataaagc agctcgtgta ggtatccgta ttgcagactt attagataaa gaaatttttg
    31141 aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt
    31201 cagaaccact tgattttgac gatatttttg aagaatatta tgaatacgga caacaaatca
    31261 aacaatacgt cacagatact tccgttatct tgaatgatgc tttggatgca ggcaaacgcg
    31321 tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga ccagggaact tatccttttg
    31381 ttacttcttc taacccagta gccggtgggg taacaatcgg aagcggtgta gggccttcaa
    31441 aaatcaataa agttgtcggc gtctgcaaag cctatacgtc acgtgtcggt gatggaccat
    31501 ttccaactga attatttgat gaaacaggag aacaaatccg cgaagtagga cgagaatacg
    31561 gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt
    31621 ctaaacgagt ttcaggtatt accaacctat ctttaaactc gatcgatgta ttaagtggat
    31681 tggaaacagt aaaaatttgt acggcttatg aattagatgg cgaactaatc taccactatc
    31741 cagcaagctt gaaagagttg aaccgctgca aaccaatcta tgaagaattg ccaggctggt
    31801 cagaagacat tacaggatgc aaaacacttg cagaactgcc agaaaacgca cgtaactatg
    31861 tacgtcgtat ctcagagctt gttggtgtac gtatctcaac attttctgtc ggacctgata
    31921 gaacacaaac aaacatctta gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac
    31981 tgaatcagga cgttcttacc agagcgagtc gaagtgttta tctatacgat aataataact
    32041 tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag tccctttttc
    32101 cgaa
//
LOCUS       DACEKQ010000024        31682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEKQ010000024 DACEKQ010000000
VERSION     DACEKQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.025"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(271..738)
                     /locus_tag="IW671_001011"
     CDS             complement(271..738)
                     /locus_tag="IW671_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0898011.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(858..2396)
                     /locus_tag="IW671_001012"
     CDS             complement(858..2396)
                     /locus_tag="IW671_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0898012.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSVMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2476..3162
                     /locus_tag="IW671_001013"
     CDS             2476..3162
                     /locus_tag="IW671_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0898013.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3363..3890)
                     /gene="msrA"
                     /locus_tag="IW671_001014"
     CDS             complement(3363..3890)
                     /gene="msrA"
                     /locus_tag="IW671_001014"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0898014.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3974..4651)
                     /gene="pgmB"
                     /locus_tag="IW671_001015"
     CDS             complement(3974..4651)
                     /gene="pgmB"
                     /locus_tag="IW671_001015"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0898015.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4648..5778)
                     /locus_tag="IW671_001016"
     CDS             complement(4648..5778)
                     /locus_tag="IW671_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0898016.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5778..7961)
                     /locus_tag="IW671_001017"
     CDS             complement(5778..7961)
                     /locus_tag="IW671_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0898017.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7958..8983)
                     /locus_tag="IW671_001018"
     CDS             complement(7958..8983)
                     /locus_tag="IW671_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0898018.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(9028..9855)
                     /locus_tag="IW671_001019"
     CDS             complement(9028..9855)
                     /locus_tag="IW671_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0898019.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9852..10919)
                     /locus_tag="IW671_001020"
     CDS             complement(9852..10919)
                     /locus_tag="IW671_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0898020.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10945..11781)
                     /locus_tag="IW671_001021"
     CDS             complement(10945..11781)
                     /locus_tag="IW671_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0898021.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11778..12647)
                     /locus_tag="IW671_001022"
     CDS             complement(11778..12647)
                     /locus_tag="IW671_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0898022.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12717..14000)
                     /locus_tag="IW671_001023"
     CDS             complement(12717..14000)
                     /locus_tag="IW671_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898023.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(14014..15699)
                     /locus_tag="IW671_001024"
     CDS             complement(14014..15699)
                     /locus_tag="IW671_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0898024.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15974..16999
                     /locus_tag="IW671_001025"
     CDS             15974..16999
                     /locus_tag="IW671_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0898025.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17594..21247)
                     /gene="rpoC"
                     /locus_tag="IW671_001026"
     CDS             complement(17594..21247)
                     /gene="rpoC"
                     /locus_tag="IW671_001026"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0898026.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21326..24943)
                     /gene="rpoB"
                     /locus_tag="IW671_001027"
     CDS             complement(21326..24943)
                     /gene="rpoB"
                     /locus_tag="IW671_001027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0898027.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25671..26639
                     /locus_tag="IW671_001028"
     CDS             25671..26639
                     /locus_tag="IW671_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898028.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26657..27562
                     /locus_tag="IW671_001029"
     CDS             26657..27562
                     /locus_tag="IW671_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898029.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27559..28365
                     /locus_tag="IW671_001030"
     CDS             27559..28365
                     /locus_tag="IW671_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898030.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28522..29490
                     /locus_tag="IW671_001031"
     CDS             28522..29490
                     /locus_tag="IW671_001031"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0898031.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29807..30331)
                     /locus_tag="IW671_001032"
     CDS             complement(29807..30331)
                     /locus_tag="IW671_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0898032.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30618..>31682
                     /gene="scm"
                     /locus_tag="IW671_001033"
     CDS             30618..>31682
                     /gene="scm"
                     /locus_tag="IW671_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0898033.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8895 a         6843 c         5965 g         9979 t
ORIGIN      
        1 tgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat
       61 aagaagctgt tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac
      241 ctctttttcg gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa
      301 atgagcttgg atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc
      361 ttgtgttgga tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc
      421 tagataacga tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg
      481 gccaatttca tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga
      541 accgtctaat gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat
      601 gaactcatcc atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt
      661 ttggtgaaca acaacagaca tttggcttag atctgcaact aattgattta atacttcttt
      721 ggtttgttgg aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt
      781 agactttcaa tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt
      841 tttatccgta aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat
      901 tgctgataag tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga
      961 tattttttat gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt
     1021 gcttcactga tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat
     1081 gcggatgctt ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc
     1141 tgacgaaaaa tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt
     1201 tcctggcaaa ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc
     1261 ttcatcattt ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct
     1321 gcgtcccaaa gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca
     1381 cccgctggca caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct
     1441 ttagcagatt gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca
     1501 ccattgctag tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc
     1561 tccatttctg gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct
     1621 acggtcaaag cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg
     1681 tctgcccctc ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt
     1741 gatggtagac ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga
     1801 atgttcaagt agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca
     1861 gttcctgaag ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag
     1921 tctttgatac cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc
     1981 cacttgattt tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat
     2041 tctttgccaa gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc
     2101 caagtgatca ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc
     2161 acagaggaga aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct
     2221 tttataacgg tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt
     2281 gtttcttgga tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg
     2341 aaagcaactg ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt
     2401 tcacctcaaa ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga
     2461 aaagggggag atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta
     2521 tgtttaaccg cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt
     2581 gtcaaatgct gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc
     2641 caacgatttc gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa
     2701 gccttatgtg gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg
     2761 acgttcatat ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg
     2821 gtccattctc acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc
     2881 ggcgtcttcc aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc
     2941 tatggtcaat cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc
     3001 ttgtctctag aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt
     3061 ttttctgttt actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt
     3121 ttgagcagtg cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac
     3181 gacttgtcac aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta
     3241 ggcgacaggt tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag
     3301 ctcaaaatgc tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct
     3361 tcttatttct tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga
     3421 taaagattgt aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga
     3481 taaaattcgc ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct
     3541 tgcaatgctt gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc
     3601 actggacgat aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt
     3661 tgctgccaat agatttccac caactgatca taagaaataa cttctggatc atattcgatc
     3721 tctactgctt ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt
     3781 acatgcccgc cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt
     3841 ttgaccatac accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc
     3901 ccttttttct catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa
     3961 aaaacggaaa ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt
     4021 gatcaaaaac ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac
     4081 ctattcccaa tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt
     4141 caatgaccaa gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt
     4201 ctggatcagg ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg
     4261 tcaaattcgt tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct
     4321 tgatgtttgt ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt
     4381 catccatttt ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt
     4441 tctctttttc tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact
     4501 cggttctgga tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct
     4561 ggttagctaa aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat
     4621 ctaaatcgaa tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg
     4681 aagtaaagat gcgatgtttt tagctgtgcg atgaagatta tcagtggctt gctgtaaagc
     4741 ttcttgtaaa ggcacagctg caggaaggat cggaaaaacg gccgaaatgc caacatctgc
     4801 tgctttttcg cttcctttac caacacttcc gcaaatagct atcacaggaa cgccctctgg
     4861 tactcttcta gcaatcccaa ctggcgcttt tccttttaga ctttgtccat ctagacgtcc
     4921 ttcgcctacg attacgaggt ctgcttgctg tacccgttta tcgaactcca gtaaatccaa
     4981 tacgtagtcg atgccagaga ctattttccc tgaagcaaat gctaacagtc ctgcacccat
     5041 tccaccacca gcactactgc ccgatgcctc gattacttga ggggcaaatc tcatataaaa
     5101 atcatgcatg gcttggtcag ccactgctaa ttcattttct ttcaagcctt tttgtggtcc
     5161 gaaaacaaaa gaagcgcctt cttttccgca taaaagatta gtaacatcag taatgacagt
     5221 gattttggct ttaatcagct caataggcac gttcgtttta tcaactgtag caattttacc
     5281 tagattttct cccagtgctt ctactttttg attatcttga tcgtaaaaag cataacctag
     5341 accttctgcc atgccgatgc cgccatcgtt ggtactactt ccgccaacgc cgatgatgat
     5401 gtgtcgaaca ccttgttgca acaattgtcg tatcagttct cctactcctc gcgtggataa
     5461 cctaagtggc tgttgttggt ttagtgggac atctttcaat ccgacgattt ctgccatttc
     5521 aaaaatcgca gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc caaatgcatt
     5581 agtaacgact gctgtttgcc gcttcaacct taaactgtct gctaatgatg aaaccgttcc
     5641 ttctcctcca tcgcctatag gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa
     5701 acctgattga atggcttgtg ctgcctctaa ggctgtcaga ctttctttga aagaatctgg
     5761 cgcaatcaga atacgcatta ttttcccctc cgttccaaga tcagttcttt ttccagctgg
     5821 tattggtctc cattgacaaa aataggtaat gtttctttcg tctctttttt tattactatt
     5881 tgtttttttg tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg aaaagaaatt
     5941 ttttcccatg ctgaaggaag tcttggagaa atagaaagag ttccattcgt tactgtaata
     6001 cctgaaaaac caaatataac tgctagccat agtgctccta atgaagcagc atgaattccg
     6061 tcatctgatg aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg gaataaatga
     6121 tacgcccatt ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc tttactcaac
     6181 gatgagtcgt ggattgtttt gttttcatag taatgcaaat tcttagaaac gattggttga
     6241 gaaaaatact caggtaataa gtataacagc atgacaagat ctgcttgctt gctgacttgg
     6301 agttcattta cttcgctacg ggcataatca agtaaaatag actggctgcc aggacgttct
     6361 ttgtaagtgg tcaaatcaat aactggctta ctgagatacg tgtcatcttg cggaatcagc
     6421 tgttctttgt taggcactgg caaatataac ttatctaaaa aattttgaca ttctttcaaa
     6481 aaaagcggat cgccagcttg atatttaaca gctaaggcca cattataatg cgccagatag
     6541 ttcgtatatg cattattgtc gatatattct gtgtattcat ctggaccaat gactctgtgt
     6601 atctctaaat gatcatcgac ttttacagct ctgctcagcc aaaaagccgc tgtttcatga
     6661 atgagcgttt ttccctttgt ttctaaaaac gattgatcct ctgttgcttg gacatactct
     6721 tgaacggcat aagcaaggtc tgcactgata tgatgttctg cggcagctga agcaacttct
     6781 tgtcttgttc cggttttgat attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg
     6841 tgtgcgcttt cccatgggaa aagtgcacct tgatagtgat aatctcttgc tttttgccgt
     6901 gcgccttcta atcgatcata acggtacagc agcaaattct ttgctacttc aggaaactga
     6961 tataaaaaga aaggcaggat aaaaatttcc gtatcccaga aaacatgccc tttataacct
     7021 tcgccagtta gtcccttcgc ccctacacta acacttggat cattgcgggg agtcataatc
     7081 tgtaaatggt aagcagcgaa gtccatcgct aactgatcga aagaagaatc gccttggatc
     7141 gttatccgat gctgatgcca aaaatctgtc caataagaca tcgactgctc taaaatttct
     7201 gtataactaa cgcttttctg ggcttgggca atacttttct taaacgggtc tgtttgctga
     7261 agagaggtag agagcacgat gatcttctcc agcgtaaagg acgtattttt tctgatcttt
     7321 tctgaaaagg aagcggtgat ccggcgattt tttgcttgga atacacctgc tttattgaat
     7381 ttagctgcaa tcccaatatc aattttactt tctgttgtct ggtaggatgc actgatcaaa
     7441 tcgtcaccaa atactcgcag ttctttttca accaactgct gtgtaccaaa attggtttgc
     7501 tgtccatcga tacccgtttt cacatctact tggcagtctt cggtcaacgg cttgatgacc
     7561 actttcaggg ctgcctgatg gagattattt tgatcagcaa cacgacaagt aatgacttcc
     7621 actttttttc cttgagcagt ttcaatgagc gcatagcgtt taagctctcc catttctaaa
     7681 tctagtgccc gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg
     7741 atttttattt cccatctgat gacatctggg agattcacta actcggctga ttctgctcct
     7801 aaaggtcggt tatatacacc agctgcaaac attcccctta cttgattccg atagtcttcc
     7861 tcctggcttc ctctgatacc aaaaaatcca tttccaatgg tcatctgact cgcatactta
     7921 ttgatatact ctaaatcaaa ctgtttttct tctactttca taaggtaata acttctcctg
     7981 tttctgccga acgatataaa ttttccacca gattctggat gacatatcct tgccagccat
     8041 ctgcaatcat ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc atacttagtt
     8101 catgtgggtc attaactggc gtggtctttt gtaaggaaac cagctttcca tctacatctt
     8161 cgaatagttc gcctggatac agattagcac ctgcctgatc gccacaaaaa gccacattca
     8221 gtttgtcttc tctgatattc aaagcaaacg aagtattcag ttggatgatc ttgccgtttt
     8281 ctaattccag tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga tcccatttcc
     8341 cgaatttccc tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt
     8401 ttactttagg aaatccagta atatacatcg ctgtatcaag catgtgaatg ccataatcga
     8461 tcaaaggccc gcctccttga agttctttgt cgataaacgt tccccagcct ggaacaccag
     8521 accgacgaag cgcattcgct tctacgtaat aaatatcccc gagaacatca agatgattct
     8581 tgatcattgt tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa atctgcccgt
     8641 ttttatcggc tgtctctgcc atctttcttg cttgatcagc tgtcatagct ggtggtttct
     8701 cgcacatcac tgaagcatta gcttcgaggg cagccaatac atgttcgaag tgaaagcgat
     8761 tgggtgtaca gacactcacg atatcgggag aaatctcatt cagcatttgt gtaactgatg
     8821 tatacgcatg agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat
     8881 caaccacagc ggccaattct aagacatctg atcttttttg ataataagca gcatgtactt
     8941 tttctgctac ttgtccagcg cctatgattg ctactttctt catttatttc tctctccttt
     9001 gcttatttac ttattgttat tctttctcta ttctttctct aaatcattta aatgacgcgc
     9061 tgtttccaga cagtttttca agtattgcaa tgattcctga taaattttca cttcattttc
     9121 tcctcgcaaa cgacattcat ataccatata accttcatac tcattttgaa gtagcgtttg
     9181 aaaatggcga gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat tatctgctaa
     9241 atggatatga cccacatact ctttttgcgt agtcaatgct tttgaaatat catcttcttc
     9301 aatattcata tgataaaaat cagcgatgat ctgcacatgt ttcaattgat tagagtcaat
     9361 gtaatgacgc gcatccgcta acaaattgat catatggtct tggtatcggt tcaaaggctc
     9421 caaataaata accgtacctg tttcttcggc tactttatct aaataacgaa gtgattcgct
     9481 gactgcttta tagtctcctt ctttgcttcg gggtgaaacc atcggcggta atcgataagt
     9541 gaacattccc caagctgctg ggataacgat tcctttgcct ccaacctcgc ttagtgcacg
     9601 caaaatctca gcaatttgac gtacaccatt aagacgccgt tcttcgataa agtcaccgat
     9661 ccacccatcg tagccgttac aagctgtcac tactgggatc cctgtttctt tgattgcctg
     9721 tttgactgtg tcgatatttt ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc
     9781 acagtctttg ataaatctaa atttttctaa aatgtctctt gggaaaaatg cttgatcttg
     9841 cgtaccgaat ttcattgttc tcctcctcgg acttgaacgc ccattttgat actttcttct
     9901 gggtgctggt ctacataacg catgtaagct tctgcgcttt ctgcaaatgg cacaactgga
     9961 tcaatgattt cttcacaatc caagtaaccg ttcatcaata attcccaaca tgtgtcctcg
    10021 attcgtttac ggttccatct tgggtaatct gggtttggtt cactagatgc tcgagcaaag
    10081 acgatttttg cattattgaa atgtgcttcg cgaccaagat taaacccagc tggaaaaggt
    10141 ttagcaaagg caacataaga aatgatcccg ccatagccga tccctcttaa agaagcttgt
    10201 aatgcggcag cactgccact cgtttcaata actgaatcta ctccacgttt atctgtcaat
    10261 tcctttagac gaaaaccgac atctgctttc ataggatcta gcgtgtaatc ggcaccgtat
    10321 ttttctgcta attctctacg ctgcgctatg ggatcgatcc caacgatggt agtagcacct
    10381 gcttttttag ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca
    10441 gcatcacctg ctcgaacatg tgcttcccgg ataccaccta aagcgaattg agcaggatcg
    10501 taacatactg catttttcca ttgatccaac cgatcaagtt tacgtagttt ataattatca
    10561 acaccgtttg taatcagcgt ttcacgaatg ggtccataag tgcagactaa ctctcctttt
    10621 tgatactctg tcaccaaact gcccgtttct tcgatcgtac cgacaatcat atttcctaat
    10681 tgaaattcgc caaataccac accgcgttgg cttccttctt ctcttggcaa gaataggcgc
    10741 cattcttctg aaaattcttc atcgataaat ggacttaatc ctctaaagtc gacaatttct
    10801 gttcctggtt ttggagtagc gaaaattact cggatacgta cttcgttctt ttcgatctca
    10861 cggtcttcgt aatctttgaa tccggctatt cttggttcta atgcaactaa ttttttcatc
    10921 ttttctttcc tctttctttt ctttttatcc ttttacgccg ccttcgacta aatcaccttt
    10981 aataaatcgt tcagataacg catacataat gatgactggt aaagcggtca ccaatgaagc
    11041 ggccatcatt cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca aaccgattgg
    11101 taaagtaaat ttttcggagg tcgacaggaa aatcgaagca aataaataat cattccacga
    11161 aaccataaag caataaacga agacagaaat gattccagaa atagccaatg gtataatgat
    11221 ccgaataatg atctggatcc ggctaagacc atcgatcatt gctgcttctt caatatcagt
    11281 cggaatcgtg tcaaaataac tttttagcat gaatatcgca gtaggaagtg tttgaacgac
    11341 catcgtgata atcagtgctg tattggtatc atacaaacct agaccggaaa tgattttaaa
    11401 taatggaaca acgatcaaaa taccggaaaa catataaacg gtgtaaaagc tagcattgat
    11461 tttcgagcga gctttgaagc gtagttttga caaagcgtat gccccgctga tcccaatcac
    11521 tactgcagtt acggaggcta tcaaggagat cactaaacta tttttgaaat acatgatgaa
    11581 tggaaaaatc tcagcattga aaatatcctg ataatgttca aaagtccatt ctttcggaaa
    11641 aatagtttgt gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca tcagcatgat
    11701 caaaaatgga aacaaattga cagctaatag caacaaaatc gtcgcgtaaa ataagatttt
    11761 cctgctcttt ttatttttca tttcaagtcc accttctttc gtgagagcaa gacgaccgct
    11821 gcaataatga tgaacaatag aatcgatata gctgctgctt ttccaagatc attgaacgca
    11881 aaggctgtct gatacaaata aacgcctaag atattgactt tattcgtcag tagatagaca
    11941 tctgtaaaca tatagaacat ccagatagat cgtaaaacaa tcacagttgc tagtactggt
    12001 ttgattgccg gcagtgtcac gatcttgaac ttctgccaag catttgcacc gtccatttca
    12061 gctgcttcat aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca
    12121 tacggatagt atctccagat agcaaacaaa acgaccataa agaagctagc aactggctgg
    12181 tcgaaccaca gcggtgcttc gttgaaaagc tgcaacctat ctactaacag atcattgacg
    12241 attccataac tattattgaa catatatttc caagcaaaaa tcaaagaaat agatggcgta
    12301 acataactta ataaaatcag tgaacgcacc gttttcctaa atctgaactt gcggttaagc
    12361 aaaatagcta cggctaatcc taaagctgta ctgcctgcta caactaagaa tgtgtaagca
    12421 actgttaaac ctaaagaacg ataaaattca ctatcactta aaatttcaag ataattgctt
    12481 aatccgataa aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa actgatctga
    12541 atattggaaa gcattggaat cagcactaaa acagctaata aaagcatgct tggtgctaaa
    12601 agtcccatgg ctaattttaa gtcagaatgt ttccttggac gattcattga aacctccccc
    12661 tgataatctg ccaatagcta tctacttttt tcgaacacca ttggcagaac ttcttgttat
    12721 ttcgtatttt tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc gacagttact
    12781 tggttcactg cttttccgat cacacctgag cttgtgatat ctcccatttt cgtgaaattc
    12841 ttttccccga ctagtccaaa gacttgtact tcatcaaaag cactagcaat ctcagatggc
    12901 agctcgccaa aagacttgat gacttcattt tctttgtaac catccagttc gactacttgt
    12961 ttattcactg gttgagcacc accaggagac atcaagaccc acttttccat gtttttcggt
    13021 tgagataaga aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc tagaccagaa
    13081 gaaatcgtca atccagatac tgtgccatag acagcttgat ctttttgtgt tgggatagca
    13141 aaaccgatat tttctgagtt ccccgcttca taaacagaag gcaaaatgta agttgaatag
    13201 attgccattg gtgctgttcc attcatgaaa gcatctttga tttctgtcac atcattagag
    13261 cctggcatcg tgtattggga taagttttga taaaacgaca atgcttctct catcttttcc
    13321 gtatcgatcg ttacctcgcc ttcatcatcc aaaacatttg ctccatttga caaagcaaat
    13381 tgagagaacg cttgctcact cattgttcct tcagcagtag gcatagcaat tccatattgt
    13441 ttattctctt tatctgtgaa atgttgagca atttttaaaa tatcatccca attttctggt
    13501 tctgaaaaac cagcttctga taatttttct ttgtcatacc aaataccttg aacccagccg
    13561 ctaattggtg cagcaatata gctttttcca tcttctgaac gaacaaggtt tttggctccg
    13621 tcataataat tatcttctcc agcttcctcg ataacgtttt ggactgccgt ctgatcaatc
    13681 agttcatctt tatccatcac tttagcaaaa tcctgactga cttccattac ttctggtaat
    13741 ttaccggaac gagctaacgt aacgattttt gtattgaacg ctgattcttc taccggcact
    13801 tgcttgattt tgatgtctgg attttctttt tcaaaatcag caaccagttc attgatcaca
    13861 cttaatcgtt cctgttcgac agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg
    13921 ttagagcttt gactttcacc acaagcagta agaagaaatc cagtagctag cagacaggta
    13981 gtaacaataa tttttttcat ttttttccac ctcttatttt attattttca gccacatata
    14041 ttcatacggc tggaatatca actttcgttc ttgactgcaa tggataattt gtccagaaaa
    14101 caggtctagg taatctcctt ttggtaaaag ataacctgct gattgattgg aaaaattatg
    14161 gataactaag actttctctt ccttcgcttc gcgaataaaa ctgataaaag aatctgctga
    14221 gtccagtaag taaatcaaac tattcggatg gaaggctttt tctttttttc gaatagatag
    14281 catctgggtc aattcaccat atactgctgt tcttaatgga tctgtctgca actctttctc
    14341 aatatctgct aacaaaaatt tttgccgatt gatttttcga ttctctcctg ttttttctac
    14401 gcctttgtaa tcatttcgac ttcccagcac actttgaata taaattgccg gcacgcctgg
    14461 taacgttacc aaaaatgaat gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc
    14521 tgttggagaa cttaacgcat ctagatacgt agcgtttatc tcatatggac tggtcgttcc
    14581 atctgggttt ttcttgtagt ttactcgggc accctctttc ttcagctgtt ccactaattc
    14641 aaggatcgtt ttttccggca gaatacctcg taccggattc aatccgatac cgtcatggga
    14701 ggctaaaaaa ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc
    14761 tttcaactta cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa
    14821 ctgatagacc atctgtgctt cattctcgcc attcccaaaa taactgatat tatctttgtg
    14881 tggcacattt gtctcagtga tcaataccgt tcctggagct gcttcatcag cgattgcacg
    14941 gaataattgg atgattttat gcgtttctgg caggtgtata caagaagttc ctagttcttt
    15001 ccataaaaat cccacagcgt ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa
    15061 aagaatatcg atcattttca ataaaacttc tggattttta aaattcaagt ctacttgatc
    15121 ttcgctaaaa gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag
    15181 taacgggctg gtccttggtc tagtcacgct gcttgtatct gtatccagtg gcaaatcaat
    15241 aaaaaaatct ttgtacgtat cattcaagtt taaatattcc tgaaaccaag tactttcggc
    15301 agacatatga ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca tttcttctat
    15361 atcttcccat gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg agaaaccatc
    15421 atcagatgta tatgggaaaa atggcaagaa atgtacaact ctaaatgtat ctgccaaata
    15481 cttttgataa aatttcttaa atgtacgcaa agttggttgc tgctcttcaa aaaattgatc
    15541 cccataagta atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg cctggccttt
    15601 cttctgctga taaacactga ctttttgttc aatcatagag gcggttttct cagacaaatc
    15661 tgagtcgcga taaatcgctt gtaaaagttt tgtatacatc ggattcccct ttcttttttg
    15721 ggagcgttcc caacgatttg atcatattat ggaaaacgct tactgtcaat agcatttttt
    15781 taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca ataattaata
    15841 gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat tttcaaaatc
    15901 atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc ccaaaaagga
    15961 ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta atgtctcaaa
    16021 atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag caaaagcaag
    16081 tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa gaggactgtc
    16141 aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata cgacctatgg
    16201 caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac aaaataactt
    16261 cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa
    16321 gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg atgaaacact
    16381 tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag tcggaaacga
    16441 ctacacgaac atctactcag tggacacgga taatttccag gatagttacg atttggtgga
    16501 aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga cagaagttta
    16561 tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc acctagatat
    16621 ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg cagcaatgaa
    16681 tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg cattaaaatc
    16741 attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg atctttcgat
    16801 cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga ctcggataga
    16861 cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac taatccacca
    16921 ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca cagattcgat
    16981 ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag gataagtcag
    17041 ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta ttttagcgaa
    17101 tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt tgtgttaaac
    17161 tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa gtttttatag
    17221 ttcgtttttt taaacacatt tacccttatt tcaatccatg ttatttgggg atacttatct
    17281 ctaaaacata aatagaataa cagacatgtc tactggtttt gttttattcg aggaattatt
    17341 caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa tttattttgc
    17401 attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag atgatcgttc
    17461 cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt ttcttctagt
    17521 tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg tcacaacctc
    17581 ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt
    17641 caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt ggttccatgt
    17701 cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata acattttctt
    17761 tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag acacgagttg
    17821 tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct tttgtgatac
    17881 ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta ttgtagtttt
    17941 gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta tcacctggat
    18001 ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc ttgtcgccga
    18061 tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag ttttcaacag
    18121 ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct gtcaatggtg
    18181 caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa ggaacagtat
    18241 acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt gcaggatctt
    18301 ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct ttcggattac
    18361 gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct ccggcaaccc
    18421 cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt gattgagcgg
    18481 cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt
    18541 aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg attgtcactg
    18601 tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat tggtttcctg
    18661 caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa cgtcccacta
    18721 aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg atttcaagac
    18781 ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg tcaaccagac
    18841 gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc cctttacgag
    18901 ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag ttcgaaatga
    18961 ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt ccggcaagct
    19021 gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag attggatttt
    19081 tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca ttccaaacag
    19141 caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat tgttttgtaa
    19201 tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc agtacactga
    19261 tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct aagtctttca
    19321 tacggtcaag catcttagaa gtttctgtta ctttgaatcg tttgaagact tctgcgatga
    19381 tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg atatgtgcag
    19441 gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc aagttgaagt
    19501 ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag atgatcttac
    19561 caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt tcacctaatg
    19621 agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc catgcgataa
    19681 cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct tcttccattg
    19741 tcaagtagta gttacctaga accatatctt gagaaggagt aacaactggt ttaccatctt
    19801 tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct tgtgcttctt
    19861 cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta taagcttcac
    19921 atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg aatgcttgga
    19981 taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt
    20041 cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc gcatttttga
    20101 tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg aataattcaa
    20161 tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca acaacgataa
    20221 cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt
    20281 tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca gtaactggac
    20341 gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata cgtttttcat
    20401 tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg ttattacggt
    20461 tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca ccttctaatt
    20521 gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc atccagcttg
    20581 gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt gcacgtgtac
    20641 gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct gcatctagat
    20701 caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg aattcttgtc
    20761 catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt ttctctaaat
    20821 tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct tccaaagcgc
    20881 gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag taccagatat
    20941 gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag
    21001 taacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt ttgtattttc
    21061 cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac aacccttcgc
    21121 gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca ccgtaagacc
    21181 agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat ttatttacat
    21241 cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg ctgatcttcg
    21301 accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt tcaaagtttt
    21361 ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct aattctttgg
    21421 cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat
    21481 cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc atgtccagac
    21541 ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct ggttttggaa
    21601 ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg tcatctgatt
    21661 tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt gcccatactt
    21721 ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa
    21781 ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt
    21841 tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct gtacgtccgt
    21901 cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt tcccatacgt
    21961 cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac tgacgtgcag
    22021 ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc acccctaaag
    22081 gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct tcttccggca
    22141 taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc ccttcatgga
    22201 ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt gacaattcat
    22261 ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg ccatgtggta
    22321 cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca tgcaatagac
    22381 gttcttctgc agataattct gttacccctt ttggcgtgac tttccctaca agcaaatcgc
    22441 catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct tttaatgcgt
    22501 cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg
    22561 cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt accaaacgac
    22621 ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa ccaactaaaa
    22681 cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc agagtatccc
    22741 ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt gttccggagt
    22801 ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg cggcgtacgc
    22861 ggatttcaga agcatcaacg tattcaacga caccgtcatg tttacataac aaagcagctc
    22921 ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct tgaggattga
    22981 tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt
    23041 tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca
    23101 tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga gccataacaa
    23161 cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct acgatgtaat
    23221 ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct gtttcacggt
    23281 ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca taactggaca
    23341 aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac atacggccat
    23401 agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc aatccgccag
    23461 gtcctaaggc tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca
    23521 tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca actggacgga
    23581 tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa
    23641 ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt tcaccgactg
    23701 aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata ccttccatca
    23761 agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt tttacagctg
    23821 cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt ggtgaaaata
    23881 ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat ggatagtaag
    23941 tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact tggtgagtca
    24001 gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat gtttcagcta
    24061 atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg taacgaccaa
    24121 cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag ttacgtgagc
    24181 tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc aaaccttctt
    24241 ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg cgtaatgttt
    24301 cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac gcacgaacta
    24361 atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg tcttttgcat
    24421 cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg aatccttctt
    24481 ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact aactgagaaa
    24541 cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt aatgggaaat
    24601 caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc aaacgcaatg
    24661 ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct actgtatact
    24721 ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg aagtcatcga
    24781 ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat tggtaagagt
    24841 ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt gcgaaacttc
    24901 tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc acccctctaa
    24961 aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa tattattttt
    25021 aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc
    25081 tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag ccgaaacgga
    25141 caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat atactacact
    25201 tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca acattttctt
    25261 aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag acctttttat
    25321 ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac caacatcaac
    25381 caaatcgcac caacacaact gaataacttt tactttagct actttagata ggtttgtatt
    25441 catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta aagaactttg
    25501 tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga aaatacaaag
    25561 taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat ccatttggac
    25621 attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa
    25681 atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc gcacaaaaag
    25741 acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa cttgtacaac
    25801 ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa gaagggtata
    25861 aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa gacaacctga
    25921 aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga atcgccactc
    25981 cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc acagcggtca
    26041 cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc aatgtgactg
    26101 gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat atcgtgccag
    26161 acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag atccaagctg
    26221 atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt
    26281 ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat ggaatctata
    26341 ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa
    26401 caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt ctagcaacag
    26461 taggaatcga ctacgaaagc ttgggcaaac aaacagggat catggcagca aaaatcttag
    26521 acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca
    26581 ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa gctgaataaa
    26641 acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg catcgcaagg
    26701 ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct tggacattgc
    26761 cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag taagtattac
    26821 aaatggctat catccattga cagcttgtct tctaggcttt ttagggggtg cggcagcagg
    26881 gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg cagggatcat
    26941 caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt
    27001 attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa aagacaacgc
    27061 tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg ttttgttctt
    27121 acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa tgagcgaagc
    27181 aaacgggatc aacacagata atatgaagat catcggttat atgatttcta atggatgcat
    27241 tgctctttct ggctccttac ttgcgcaaaa caacggcttt gctgatttaa attctggtat
    27301 tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt tacgtaacca
    27361 gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc gttttatttt
    27421 agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt ctgctttcgt
    27481 gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa
    27541 gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca tcataccttt
    27601 gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc cttagatccc
    27661 ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt gctgaacagc
    27721 atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa aaatatcacg
    27781 aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta
    27841 gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa
    27901 aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa agaccagctg
    27961 gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga
    28021 ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga actgatctta
    28081 ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat ggaattgaca
    28141 gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga catggaagac
    28201 gcgatccgct atgggagtcg actgatcatg ctgcatcaag gaaggatcgt cgtagatatc
    28261 cagcaagagg aaaaacagca gctcacagtg ccccaattat tagcgttgtt caaacaaaac
    28321 agcggcacgg cattaaaaga cgatcaagta cttttatctt cataatcaga aagaggatgg
    28381 gacaaaaaat gaacgcccac cctcttcttt tttctctttc cttctttcgt ttttgcttta
    28441 tgtatcaacc gttgtttctt cgaaaactat tttgttctct ccttcttcct gatagaatgg
    28501 atgggaaagg agcttgatgc catgacaacg aaagacaagg tacttcaatt attgaaaaag
    28561 acaccagatt ttctttccgg agaaaagttg gcacagcaac tggaagtttc ccggacaagt
    28621 atttggaaag ccatcaaaga attggaaaag gcaggctatc gttttgagca cgggcctaca
    28681 gggtatcgtt acctgccctc tgacgtacta gatgcaaaag aaatccagca agagctacgg
    28741 tcgtttattc ctgatttgaa agtacaaacg atgttgactt ctgaatctac gatgaaagat
    28801 gccaaactgg cagcagctca aggagattct gtttcgaagt tatttgttgc tgatacacaa
    28861 gaacaagctc gtggacgttt tggacggcct tttttcgctg aacctggacg aggaatctac
    28921 atgagtctct tgctccatcc agacaaacgg tttgatgaat tgcctcaata tacgatcatc
    28981 gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa
    29041 tgggtcaatg atatctacat ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc
    29101 agtgatatgg aaagcggcag gatcagtcat atcatcattg ggatcgggct taatttttct
    29161 gttcctcaaa atcattttcc agctgatctc caagaaaaag ccatctctgt ctttcccgac
    29221 ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga attgttcact
    29281 tatctttctc acccagaaaa agcgttggac ttctataaag aaaaatcatt cgtattggga
    29341 aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt
    29401 gatcatggcg aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa
    29461 atcagtctca catctatccg tacgcaataa ttatcagatt tcacaaaagg acacctgatc
    29521 tcatacagtg tccttttttc tgaattctct tcaaaagact ctccttattc tatcagctga
    29581 cagtttctct atcctctaga aaaaggtatt ctaaaaatat ctataaagca tggtacactt
    29641 tctttaaatg aatcaatgca aaaaacaaga gagtcatcag tttacacgat caaaagagat
    29701 agcaggccgc ttcctttttc aatcttatag atagaccatg ggtaacgata aaaacaaaaa
    29761 agaacttgcg acttattttg ccgtaagttc ttttttagct agtgccttaa tctcttaatc
    29821 ccaacaaacg tctcgcatag atcacctgac cagagtgcat agcagcatca tcaataatag
    29881 aaacgagacg cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta
    29941 gctcggaagg ttccagcctt cttaagtaag aaatagcaaa atcagcagct cctcgcaagt
    30001 atcctagcag atcttctatt tgattagctt ggatctgctt tgcttgttcc agactatgct
    30061 tccaatcttg ttcgttatct gctatcgtca tcggaaaccg gctttcccag tcttgactat
    30121 gccaaattgg agaagttcct gctaaatcag caatctggaa atctaattct cgagcggtat
    30181 gccaagccag ccaagtcatt gatttgatct gcggggctct atcttccact ggaaaacgat
    30241 tggcctcctc aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca
    30301 cactctcgca tagcatatct aaataatcca tttattttcc tcctttttat ttatcctact
    30361 tactttttcc taattctagg atacctactt atatgttact acacaccctt attttttaca
    30421 ctttaacagt caccatcatc aatagtttgg agattgaaag aaaaagatag tgtcgaacta
    30481 tcaattgaac aagaagcaac aatatgaacg tttcattaat attctttaaa taaacaatga
    30541 tttttcaaac gaacagttta agactaggag aaaaagagta taataggaaa atgtttgaaa
    30601 ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact agtcctagtt gctttgttgt
    30661 tcaatatctt ttcgtatggt gtggccgttt cagcggaaac atacattcct aaagaccctg
    30721 ggaatattta taatgatcta actggtaact atggcttgtt aggaattgct agtcaattcc
    30781 acgtatttgc taaaaataag acaactgtga atgcacatac agacggaaat atagcaacac
    30841 atgaacttga tgcaaaaaat aattttggta cagagcttca tgaaggttta ttgaaccaag
    30901 aaataaatta tatacaaaaa actgattctt tcgctaattc ttcaggcata cctacaggta
    30961 gcacaagaat gaataaattt gtcgttggca gtaacgtgga tgtagcactt caagatggtc
    31021 atcctacagt caatggatcc aggcttgatc acttaactcc agatgatttt tataaagatc
    31081 gtgctgggca tgaatatatt gatttcgagc aggaatttgc taaattgaat gtagcagcaa
    31141 atgatcttgc aacgatcact ccagctaaaa catatactgc tgcagatttt ccagacatga
    31201 acaatcggac aatcgattta actgatttat cagataccgg gtttttattg gtaaatatcg
    31261 atgcagaagt gttaacgatg aatacgccac ttcaaatcat taatccaaat gatcaagtag
    31321 ttgtatttaa tgtcatcaac agttcttctg cattgaacgt acagtcacca atcaaataca
    31381 atgatcgttc aaatcatgaa acagaagatt tttcggatgc gaatatctct tggaattttg
    31441 gaaatgagat gactgatttg acaatcagtg ctccttttca aggaacaatc cttgcgccta
    31501 atgctactat ccgcattact caaaaccaag acggaacgat tataggaaca aatgtgattt
    31561 tagatgcagc taccaatcgt tgggatccta acgaaatatt tatcagcgac aatggtacag
    31621 atacgacaga cacaagcgac agtacagata catctaccac aagtgatagc tcagattcat
    31681 ca
//
LOCUS       DACEKQ010000025        30549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEKQ010000025 DACEKQ010000000
VERSION     DACEKQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.026"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW671_001034"
     CDS             147..1016
                     /locus_tag="IW671_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0898034.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW671_001035"
     CDS             1225..2250
                     /locus_tag="IW671_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0898035.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW671_001036"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW671_001036"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0898036.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IW671_001037"
     CDS             3333..5036
                     /locus_tag="IW671_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898037.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGEIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW671_001038"
     CDS             5038..7071
                     /locus_tag="IW671_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0898038.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW671_001039"
     CDS             7082..7522
                     /locus_tag="IW671_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0898039.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW671_001040"
     CDS             complement(7722..8849)
                     /locus_tag="IW671_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898040.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW671_001041"
     CDS             complement(8852..9529)
                     /locus_tag="IW671_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898041.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW671_001042"
     CDS             9793..11559
                     /locus_tag="IW671_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0898042.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW671_001043"
     CDS             11574..12011
                     /locus_tag="IW671_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0898043.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW671_001044"
     CDS             12024..13175
                     /locus_tag="IW671_001044"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0898044.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IW671_001045"
     CDS             complement(13237..13935)
                     /locus_tag="IW671_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0898045.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IW671_001046"
     CDS             complement(13928..15280)
                     /locus_tag="IW671_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0898046.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IW671_001047"
     CDS             complement(15503..16903)
                     /locus_tag="IW671_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0898047.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IW671_001048"
     CDS             17064..17801
                     /locus_tag="IW671_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0898048.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IW671_001049"
     CDS             17926..18405
                     /locus_tag="IW671_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0898049.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IW671_001050"
     CDS             18402..19169
                     /locus_tag="IW671_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898050.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IW671_001051"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IW671_001051"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0898051.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IW671_001052"
     CDS             complement(20072..20674)
                     /locus_tag="IW671_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0898052.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IW671_001053"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IW671_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IW671_001054"
     CDS             21355..21939
                     /locus_tag="IW671_001054"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0898053.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IW671_001055"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IW671_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0898054.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IW671_001056"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IW671_001056"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0898055.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IW671_001057"
     CDS             23840..24019
                     /locus_tag="IW671_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898056.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IW671_001058"
     CDS             24041..25066
                     /locus_tag="IW671_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0898057.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IW671_001059"
     CDS             25241..26485
                     /locus_tag="IW671_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0898058.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IW671_001060"
     CDS             26638..27585
                     /locus_tag="IW671_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0898059.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IW671_001061"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IW671_001061"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0898060.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IW671_001062"
     CDS             complement(28487..28729)
                     /locus_tag="IW671_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898061.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IW671_001063"
     tRNA            28850..28922
                     /locus_tag="IW671_001063"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IW671_001064"
     CDS             complement(29041..29679)
                     /locus_tag="IW671_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0898062.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="IW671_001065"
     CDS             complement(29802..30434)
                     /locus_tag="IW671_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0898063.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10397 a         5371 c         6168 g         8613 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacctaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgaaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctctt
//
LOCUS       DACEKQ010000026        29718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEKQ010000026 DACEKQ010000000
VERSION     DACEKQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.027"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..870)
                     /gene="proC"
                     /locus_tag="IW671_001066"
     CDS             complement(64..870)
                     /gene="proC"
                     /locus_tag="IW671_001066"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0898064.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            1011..3584
                     /gene="mprF"
                     /locus_tag="IW671_001067"
     CDS             1011..3584
                     /gene="mprF"
                     /locus_tag="IW671_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0898065.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3738..4589
                     /locus_tag="IW671_001068"
     CDS             3738..4589
                     /locus_tag="IW671_001068"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0898066.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            4834..5772
                     /locus_tag="IW671_001069"
     CDS             4834..5772
                     /locus_tag="IW671_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898067.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            5795..6475
                     /locus_tag="IW671_001070"
     CDS             5795..6475
                     /locus_tag="IW671_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898068.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            6448..7272
                     /locus_tag="IW671_001071"
     CDS             6448..7272
                     /locus_tag="IW671_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0898069.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            7439..8257
                     /gene="purR"
                     /locus_tag="IW671_001072"
     CDS             7439..8257
                     /gene="purR"
                     /locus_tag="IW671_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0898070.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            8760..10133
                     /gene="glmU"
                     /locus_tag="IW671_001073"
     CDS             8760..10133
                     /gene="glmU"
                     /locus_tag="IW671_001073"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0898071.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            10227..11198
                     /locus_tag="IW671_001074"
     CDS             10227..11198
                     /locus_tag="IW671_001074"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0898072.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      11204..11306
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11372..11941
                     /gene="thiT"
                     /locus_tag="IW671_001075"
     CDS             11372..11941
                     /gene="thiT"
                     /locus_tag="IW671_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0898073.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(12022..12795)
                     /locus_tag="IW671_001076"
     CDS             complement(12022..12795)
                     /locus_tag="IW671_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898074.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            13061..14761
                     /locus_tag="IW671_001077"
     CDS             13061..14761
                     /locus_tag="IW671_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0898075.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            14731..14949
                     /locus_tag="IW671_001078"
     CDS             14731..14949
                     /locus_tag="IW671_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898076.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            14969..15859
                     /locus_tag="IW671_001079"
     CDS             14969..15859
                     /locus_tag="IW671_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0898077.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKTREQVQNIE"
     gene            15877..16161
                     /locus_tag="IW671_001080"
     CDS             15877..16161
                     /locus_tag="IW671_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898078.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            16648..17262
                     /gene="gmk"
                     /locus_tag="IW671_001081"
     CDS             16648..17262
                     /gene="gmk"
                     /locus_tag="IW671_001081"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0898079.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            17267..17572
                     /locus_tag="IW671_001082"
     CDS             17267..17572
                     /locus_tag="IW671_001082"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0898080.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            17825..20233
                     /gene="priA"
                     /locus_tag="IW671_001083"
     CDS             17825..20233
                     /gene="priA"
                     /locus_tag="IW671_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0898081.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            20248..20739
                     /gene="def"
                     /locus_tag="IW671_001084"
     CDS             20248..20739
                     /gene="def"
                     /locus_tag="IW671_001084"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0898082.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            20732..21670
                     /locus_tag="IW671_001085"
     CDS             20732..21670
                     /locus_tag="IW671_001085"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0898083.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            21670..23028
                     /gene="rsmB"
                     /locus_tag="IW671_001086"
     CDS             21670..23028
                     /gene="rsmB"
                     /locus_tag="IW671_001086"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0898084.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            23041..23781
                     /locus_tag="IW671_001087"
     CDS             23041..23781
                     /locus_tag="IW671_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0898085.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            23778..25847
                     /gene="pknB"
                     /locus_tag="IW671_001088"
     CDS             23778..25847
                     /gene="pknB"
                     /locus_tag="IW671_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0898086.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            26012..26911
                     /gene="rsgA"
                     /locus_tag="IW671_001089"
     CDS             26012..26911
                     /gene="rsgA"
                     /locus_tag="IW671_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0898087.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            26924..27574
                     /locus_tag="IW671_001090"
     CDS             26924..27574
                     /locus_tag="IW671_001090"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0898088.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            27575..28213
                     /locus_tag="IW671_001091"
     CDS             27575..28213
                     /locus_tag="IW671_001091"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0898089.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            28368..29222
                     /gene="mreC"
                     /locus_tag="IW671_001092"
     CDS             28368..29222
                     /gene="mreC"
                     /locus_tag="IW671_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0898090.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            29226..>29718
                     /gene="mreD"
                     /locus_tag="IW671_001093"
     CDS             29226..>29718
                     /gene="mreD"
                     /locus_tag="IW671_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0898091.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
BASE COUNT         9680 a         5278 c         6453 g         8307 t
ORIGIN      
        1 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
       61 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
      121 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
      181 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
      241 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
      301 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
      361 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
      421 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
      481 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
      541 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
      601 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
      661 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
      721 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
      781 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
      841 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
      901 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
      961 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     1021 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     1081 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     1141 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     1201 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     1261 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     1321 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     1381 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     1441 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     1501 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     1561 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     1621 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     1681 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     1741 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     1801 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     1861 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     1921 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     1981 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     2041 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     2101 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     2161 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     2221 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     2281 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     2341 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     2401 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     2461 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     2521 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     2581 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     2641 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     2701 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     2761 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     2821 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     2881 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     2941 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     3001 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     3061 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     3121 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     3181 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     3241 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     3301 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     3361 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
     3421 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
     3481 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
     3541 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
     3601 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
     3661 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
     3721 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
     3781 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
     3841 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
     3901 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
     3961 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
     4021 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
     4081 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
     4141 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
     4201 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
     4261 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
     4321 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
     4381 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
     4441 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
     4501 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
     4561 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
     4621 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
     4681 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
     4741 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
     4801 aattccgatt tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg
     4861 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
     4921 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
     4981 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
     5041 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
     5101 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
     5161 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
     5221 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
     5281 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
     5341 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
     5401 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
     5461 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
     5521 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
     5581 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
     5641 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
     5701 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
     5761 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
     5821 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
     5881 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
     5941 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
     6001 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
     6061 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
     6121 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
     6181 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
     6241 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
     6301 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
     6361 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
     6421 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
     6481 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
     6541 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
     6601 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
     6661 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
     6721 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
     6781 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
     6841 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
     6901 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
     6961 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
     7021 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
     7081 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
     7141 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
     7201 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
     7261 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
     7321 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
     7381 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
     7441 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
     7501 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
     7561 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
     7621 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
     7681 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
     7741 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
     7801 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
     7861 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
     7921 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
     7981 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
     8041 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
     8101 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
     8161 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
     8221 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
     8281 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
     8341 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
     8401 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
     8461 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
     8521 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
     8581 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
     8641 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
     8701 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
     8761 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
     8821 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
     8881 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
     8941 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
     9001 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
     9061 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
     9121 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
     9181 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
     9241 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
     9301 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
     9361 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
     9421 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
     9481 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
     9541 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
     9601 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
     9661 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
     9721 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
     9781 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
     9841 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
     9901 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
     9961 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    10021 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    10081 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    10141 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    10201 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    10261 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    10321 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    10381 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    10441 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    10501 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    10561 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    10621 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    10681 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    10741 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    10801 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    10861 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    10921 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    10981 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    11041 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    11101 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    11161 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    11221 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    11281 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    11341 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    11401 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    11461 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    11521 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    11581 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    11641 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    11701 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    11761 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    11821 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    11881 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    11941 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    12001 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    12061 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    12121 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    12181 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    12241 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    12301 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    12361 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    12421 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    12481 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    12541 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    12601 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    12661 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    12721 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    12781 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    12841 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    12901 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    12961 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    13021 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    13081 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    13141 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    13201 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    13261 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    13321 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    13381 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    13441 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    13501 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    13561 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    13621 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    13681 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    13741 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    13801 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    13861 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    13921 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    13981 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    14041 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    14101 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    14161 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    14221 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    14281 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    14341 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    14401 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    14461 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    14521 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    14581 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    14641 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    14701 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    14761 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    14821 agctggaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    14881 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    14941 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    15001 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    15061 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    15121 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    15181 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    15241 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    15301 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    15361 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    15421 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    15481 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    15541 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    15601 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    15661 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    15721 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    15781 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa ccagagaaca
    15841 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    15901 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    15961 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    16021 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    16081 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    16141 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    16201 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    16261 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    16321 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    16381 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    16441 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    16501 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    16561 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    16621 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    16681 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    16741 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    16801 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    16861 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    16921 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    16981 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    17041 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    17101 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    17161 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    17221 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    17281 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    17341 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    17401 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    17461 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    17521 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    17581 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    17641 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    17701 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    17761 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    17821 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    17881 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    17941 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    18001 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    18061 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    18121 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    18181 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    18241 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    18301 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    18361 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    18421 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    18481 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    18541 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    18601 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    18661 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    18721 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    18781 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    18841 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    18901 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    18961 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    19021 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    19081 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    19141 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    19201 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    19261 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    19321 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    19381 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    19441 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    19501 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    19561 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    19621 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    19681 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    19741 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    19801 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    19861 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    19921 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    19981 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    20041 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    20101 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    20161 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    20221 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    20281 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    20341 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    20401 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    20461 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    20521 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    20581 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    20641 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    20701 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    20761 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    20821 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    20881 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    20941 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    21001 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    21061 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    21121 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    21181 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    21241 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    21301 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    21361 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    21421 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    21481 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    21541 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    21601 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    21661 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    21721 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    21781 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    21841 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    21901 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    21961 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    22021 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    22081 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    22141 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    22201 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    22261 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    22321 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    22381 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    22441 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    22501 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    22561 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    22621 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    22681 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    22741 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    22801 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    22861 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    22921 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    22981 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    23041 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    23101 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    23161 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    23221 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    23281 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    23341 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    23401 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    23461 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    23521 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    23581 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    23641 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    23701 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    23761 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    23821 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    23881 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    23941 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    24001 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    24061 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    24121 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    24181 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    24241 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    24301 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    24361 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    24421 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    24481 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    24541 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    24601 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    24661 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    24721 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    24781 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    24841 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    24901 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    24961 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    25021 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    25081 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    25141 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    25201 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    25261 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    25321 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    25381 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    25441 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    25501 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    25561 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    25621 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    25681 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    25741 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    25801 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    25861 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    25921 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    25981 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    26041 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    26101 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    26161 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    26221 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    26281 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    26341 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    26401 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    26461 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    26521 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    26581 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    26641 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    26701 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    26761 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    26821 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    26881 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    26941 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    27001 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    27061 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    27121 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    27181 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    27241 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    27301 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    27361 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    27421 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    27481 tgcaggggcg gatgtcttcg ttgcaggatc atatatctat ggagccgaaa atccagtcgg
    27541 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    27601 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    27661 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    27721 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    27781 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    27841 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    27901 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    27961 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    28021 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    28081 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    28141 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    28201 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    28261 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    28321 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    28381 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    28441 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    28501 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    28561 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    28621 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    28681 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    28741 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    28801 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    28861 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    28921 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    28981 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    29041 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    29101 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    29161 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    29221 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    29281 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    29341 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    29401 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    29461 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    29521 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    29581 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    29641 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    29701 tttttccctt taagaaat
//
LOCUS       DACEKQ010000027        29155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEKQ010000027 DACEKQ010000000
VERSION     DACEKQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.028"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW671_001094"
     CDS             complement(<1..1031)
                     /locus_tag="IW671_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0898092.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IW671_001095"
     CDS             complement(1113..1802)
                     /locus_tag="IW671_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0898093.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW671_001096"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW671_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0898094.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IW671_001097"
     CDS             complement(2329..2832)
                     /locus_tag="IW671_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0898095.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW671_001098"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW671_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0898096.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW671_001099"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW671_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0898097.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW671_001100"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW671_001100"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0898098.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW671_001101"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW671_001101"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0898099.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IW671_001102"
     CDS             complement(6444..7463)
                     /locus_tag="IW671_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0898100.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IW671_001103"
     CDS             complement(7636..8523)
                     /locus_tag="IW671_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0898101.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW671_001104"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW671_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0898102.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW671_001105"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW671_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0898103.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW671_001106"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW671_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0898104.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IW671_001107"
     CDS             9754..10398
                     /locus_tag="IW671_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0898105.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IW671_001108"
     CDS             complement(10454..10957)
                     /locus_tag="IW671_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0898106.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IW671_001109"
     CDS             11554..12519
                     /locus_tag="IW671_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0898107.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IW671_001110"
     CDS             complement(12618..13790)
                     /locus_tag="IW671_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898108.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IW671_001111"
     CDS             complement(13777..14922)
                     /locus_tag="IW671_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898109.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IW671_001112"
     CDS             complement(14936..15868)
                     /locus_tag="IW671_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898110.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IW671_001113"
     CDS             15985..17064
                     /locus_tag="IW671_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0898111.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IW671_001114"
     CDS             17061..17690
                     /locus_tag="IW671_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0898112.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IW671_001115"
     CDS             complement(17800..18027)
                     /locus_tag="IW671_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0898113.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IW671_001116"
     CDS             complement(18130..20718)
                     /locus_tag="IW671_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0898114.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IW671_001117"
     CDS             complement(20738..21397)
                     /locus_tag="IW671_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0898115.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW671_001118"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW671_001118"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0898116.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IW671_001119"
     CDS             complement(22170..23036)
                     /locus_tag="IW671_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0898117.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IW671_001120"
     CDS             23145..23786
                     /locus_tag="IW671_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0898118.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW671_001121"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW671_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0898119.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IW671_001122"
     CDS             complement(25227..26126)
                     /locus_tag="IW671_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0898120.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IW671_001123"
     CDS             complement(26128..26925)
                     /locus_tag="IW671_001123"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0898121.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IW671_001124"
     CDS             complement(26903..27607)
                     /locus_tag="IW671_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0898122.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IW671_001125"
     CDS             27738..28313
                     /locus_tag="IW671_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0898123.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IW671_001126"
     CDS             28431..29108
                     /locus_tag="IW671_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0898124.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8583 a         6173 c         5242 g         9157 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa aaggctcttt gtcaaatgat gttggtaaag agaaatctta gaata
//
LOCUS       DACEKQ010000028        28512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEKQ010000028 DACEKQ010000000
VERSION     DACEKQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.029"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(218..1015)
                     /locus_tag="IW671_001127"
     CDS             complement(218..1015)
                     /locus_tag="IW671_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0898125.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IW671_001128"
     CDS             complement(1012..2196)
                     /locus_tag="IW671_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0898126.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IW671_001129"
     CDS             complement(2221..3468)
                     /locus_tag="IW671_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0898127.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IW671_001130"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IW671_001130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0898128.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IW671_001131"
     CDS             5448..5762
                     /locus_tag="IW671_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0898129.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IW671_001132"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IW671_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0898130.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IW671_001133"
     CDS             6447..7091
                     /locus_tag="IW671_001133"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0898131.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IW671_001134"
     CDS             7106..7435
                     /locus_tag="IW671_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898132.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IW671_001135"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IW671_001135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0898133.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IW671_001136"
     CDS             8423..9247
                     /locus_tag="IW671_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0898134.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IW671_001137"
     CDS             9240..9587
                     /locus_tag="IW671_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0898135.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IW671_001138"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IW671_001138"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0898136.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IW671_001139"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IW671_001139"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0898137.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW671_001140"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW671_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0898138.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW671_001141"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW671_001141"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0898139.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IW671_001142"
     CDS             15244..16011
                     /locus_tag="IW671_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898140.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IW671_001143"
     CDS             16001..18043
                     /locus_tag="IW671_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898141.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGITQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IW671_001144"
     CDS             18324..18473
                     /locus_tag="IW671_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0898142.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IW671_001145"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IW671_001145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0898143.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IW671_001146"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IW671_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0898144.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IW671_001147"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IW671_001147"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0898145.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IW671_001148"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IW671_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0898146.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IW671_001149"
     CDS             22890..23972
                     /locus_tag="IW671_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0898147.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IW671_001150"
     CDS             23972..24775
                     /locus_tag="IW671_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0898148.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IW671_001151"
     CDS             24845..25585
                     /locus_tag="IW671_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0898149.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IW671_001152"
     CDS             25596..26600
                     /locus_tag="IW671_001152"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0898150.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IW671_001153"
     CDS             complement(26645..28489)
                     /locus_tag="IW671_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898151.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8993 a         5040 c         5863 g         8616 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattacaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac tt
//
LOCUS       DACEKQ010000029        27663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEKQ010000029 DACEKQ010000000
VERSION     DACEKQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.030"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1096
                     /locus_tag="IW671_001154"
     CDS             <1..1096
                     /locus_tag="IW671_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0898152.1"
                     /translation="LEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLARSVKL
                     HYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANLFLIF
                     YLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAAVPFK
                     KGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAGYQMT
                     HSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLLFSLC
                     MRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYGGTSY
                     LILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1188..1943
                     /locus_tag="IW671_001155"
     CDS             1188..1943
                     /locus_tag="IW671_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0898153.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2001..4040
                     /locus_tag="IW671_001156"
     CDS             2001..4040
                     /locus_tag="IW671_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0898154.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4149..5300)
                     /locus_tag="IW671_001157"
     CDS             complement(4149..5300)
                     /locus_tag="IW671_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0898155.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5454..5984)
                     /locus_tag="IW671_001158"
     CDS             complement(5454..5984)
                     /locus_tag="IW671_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898156.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6083..6433)
                     /locus_tag="IW671_001159"
     CDS             complement(6083..6433)
                     /locus_tag="IW671_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0898157.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6590..8119)
                     /gene="ahpF"
                     /locus_tag="IW671_001160"
     CDS             complement(6590..8119)
                     /gene="ahpF"
                     /locus_tag="IW671_001160"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0898158.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8129..8692)
                     /gene="ahpC"
                     /locus_tag="IW671_001161"
     CDS             complement(8129..8692)
                     /gene="ahpC"
                     /locus_tag="IW671_001161"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0898159.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(8836..9498)
                     /locus_tag="IW671_001162"
     CDS             complement(8836..9498)
                     /locus_tag="IW671_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0898160.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            9682..11328
                     /locus_tag="IW671_001163"
     CDS             9682..11328
                     /locus_tag="IW671_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0898161.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11319..12401
                     /gene="uxuA"
                     /locus_tag="IW671_001164"
     CDS             11319..12401
                     /gene="uxuA"
                     /locus_tag="IW671_001164"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0898162.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12509..14062)
                     /locus_tag="IW671_001165"
                     /pseudo
     CDS             complement(12509..14062)
                     /locus_tag="IW671_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14210..15351
                     /locus_tag="IW671_001166"
                     /pseudo
     CDS             14210..15351
                     /locus_tag="IW671_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15477..16556)
                     /locus_tag="IW671_001167"
     CDS             complement(15477..16556)
                     /locus_tag="IW671_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0898163.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(16659..17099)
                     /locus_tag="IW671_001168"
     CDS             complement(16659..17099)
                     /locus_tag="IW671_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0898164.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17156..17566)
                     /locus_tag="IW671_001169"
     CDS             complement(17156..17566)
                     /locus_tag="IW671_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0898165.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(17746..18180)
                     /locus_tag="IW671_001170"
     CDS             complement(17746..18180)
                     /locus_tag="IW671_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0898166.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18280..19128)
                     /locus_tag="IW671_001171"
     CDS             complement(18280..19128)
                     /locus_tag="IW671_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0898167.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19517..21283
                     /locus_tag="IW671_001172"
     CDS             19517..21283
                     /locus_tag="IW671_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0898168.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21385..22644
                     /locus_tag="IW671_001173"
     CDS             21385..22644
                     /locus_tag="IW671_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0898169.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            22791..24092
                     /locus_tag="IW671_001174"
     CDS             22791..24092
                     /locus_tag="IW671_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0898170.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24095..24946
                     /locus_tag="IW671_001175"
     CDS             24095..24946
                     /locus_tag="IW671_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0898171.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            24959..25774
                     /locus_tag="IW671_001176"
     CDS             24959..25774
                     /locus_tag="IW671_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0898172.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            25925..27091
                     /locus_tag="IW671_001177"
     CDS             25925..27091
                     /locus_tag="IW671_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0898173.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            complement(27272..>27663)
                     /locus_tag="IW671_001178"
     CDS             complement(27272..>27663)
                     /locus_tag="IW671_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0898174.1"
                     /translation="RNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQAFHDKEAD
                     TFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTHIKALKRVA
                     YGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         9268 a         4999 c         5180 g         8216 t
ORIGIN      
        1 tttagaagta tacagtgcca gttcttatcg tttattgcaa gccgatgaaa atacaaaaag
       61 tctattattg cgtcaactaa ttttcatatt tttgagttgg agcgtgatct tcttagctcg
      121 ttcagtcaaa ctacactatt tacttcaccc taaaatagca ggatacggtt tagccttatc
      181 gattttcttt ttagtattag taagaatagg gatattcggt gtcactgtca acggcgcaca
      241 acgttggatc tctctgtttg gcattcaatt ccagccttct gaactggcaa atcttttttt
      301 gattttttat ttaagctggt tttttcgtga cggaaatagt agcccaaaag atctaaaaaa
      361 accattcctg attacagtag gtataacttt tctgatttta tttcagccaa agattgctgg
      421 agcattgatg atcctttcga ttgcgtgggt catattttgg gcagcggcgg ttccatttaa
      481 aaaagggatc tatctaatcg ttactttttc tgcattgctg attggagcag caggcggggt
      541 attatattta ggaaataaag gttggcttcc acagatgttt aatcatgctt atgaaaggat
      601 cgcgacttta agagattctt ttatcgatag tcatggtgcg ggctatcaaa tgacccattc
      661 gttttatgca ctatataatg gtggcatatt tgggcgggga ttaggaaata gtatcacaaa
      721 aaaagggtat cttccagaga cagaaacaga ttttattttt tcgattatta cagaagagct
      781 tgggctaatc ggtgcattgt gtgtgctttt cttattgttc tcattatgca tgagaatctt
      841 ttgtttaagc agtcgctgta aaaatcagca agctggattg tttcttttag gattcggaac
      901 tttgctattc gttcagacga taatgaacgt gggaagtatc gcggggctga tgccaatgac
      961 tggtgtgcca ttgccatttg ttagctacgg aggaacaagt tatctgatcc tttctttagg
     1021 aatcggaatc acgttgaata tttcttcaaa gatacaggcc gaagagttgg cgctctatcg
     1081 acctgaaaaa caataaagag aaactgagaa actctcagtt tgaattatca taaagtacaa
     1141 tcttttattt ttcaagcgag aggttctttt agaaagagga attcgcaatg aaagtatctc
     1201 aaaaaatcat attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg
     1261 agtttatcca tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga
     1321 aaagtatccc taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg
     1381 aaaacggtcg ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa
     1441 aactgatctc tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga
     1501 taaatgcagc ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt
     1561 tcaaatttga agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta
     1621 atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg
     1681 tatatataaa caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat
     1741 ttcgggaaga caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag
     1801 ctatctctca gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga
     1861 aattacttgg tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg
     1921 atttcctcaa agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca
     1981 agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg
     2041 cttatattgc cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc
     2101 agcactatca agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc
     2161 aagctttgaa caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa
     2221 ataaaagtgc attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg
     2281 ctgatgtcaa aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt
     2341 acagtttttc atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt
     2401 ataaaggaac attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg
     2461 tttttccaga aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag
     2521 ggaatattat agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag
     2581 gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg
     2641 cttcctcctt cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac
     2701 aacccgatta ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg
     2761 gagctaccat tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc
     2821 tgattggtta cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca
     2881 gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga
     2941 caacaggtgg gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg
     3001 aacatgaagt tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa
     3061 cagcttttga cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg
     3121 gtgatcttct tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga
     3181 tctcacaaga agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat
     3241 ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg
     3301 acaacggcac tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag
     3361 acagttcttg gggatcttat caagcaacgc gtgtcagtag tgatgtatca caagtagact
     3421 taaaaactgc tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg
     3481 gtgagaaaaa atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc
     3541 caattagtat gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc
     3601 tagctgatac tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa
     3661 tgtattctgt ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag
     3721 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
     3781 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
     3841 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
     3901 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
     3961 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
     4021 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
     4081 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
     4141 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
     4201 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
     4261 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
     4321 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
     4381 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
     4441 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
     4501 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
     4561 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
     4621 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
     4681 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
     4741 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
     4801 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
     4861 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
     4921 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
     4981 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
     5041 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
     5101 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
     5161 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
     5221 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
     5281 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
     5341 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
     5401 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
     5461 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
     5521 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
     5581 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
     5641 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
     5701 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
     5761 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
     5821 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
     5881 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
     5941 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
     6001 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
     6061 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
     6121 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
     6181 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
     6241 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
     6301 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
     6361 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
     6421 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
     6481 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc
     6541 ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca
     6601 tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt
     6661 gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc
     6721 catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat
     6781 ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct
     6841 ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt
     6901 tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga
     6961 tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag
     7021 ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc
     7081 ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt
     7141 tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg
     7201 ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg
     7261 aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt
     7321 gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg
     7381 tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg
     7441 ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag
     7501 gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt
     7561 cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca
     7621 tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa
     7681 tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac
     7741 aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac
     7801 tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag
     7861 aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg
     7921 gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag
     7981 acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca
     8041 aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt
     8101 tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt
     8161 tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta
     8221 catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg
     8281 cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag
     8341 ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac
     8401 cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag
     8461 taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt
     8521 attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga
     8581 agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa aattctcctt
     8641 gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac
     8701 ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg
     8761 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
     8821 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
     8881 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
     8941 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
     9001 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
     9061 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
     9121 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
     9181 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
     9241 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
     9301 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
     9361 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
     9421 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
     9481 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
     9541 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
     9601 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
     9661 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
     9721 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
     9781 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
     9841 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
     9901 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
     9961 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
    10021 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
    10081 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
    10141 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
    10201 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
    10261 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
    10321 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
    10381 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
    10441 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
    10501 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
    10561 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
    10621 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
    10681 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
    10741 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
    10801 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
    10861 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
    10921 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
    10981 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
    11041 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
    11101 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
    11161 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
    11221 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
    11281 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
    11341 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
    11401 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
    11461 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
    11521 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
    11581 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
    11641 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
    11701 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
    11761 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
    11821 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
    11881 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
    11941 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
    12001 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
    12061 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
    12121 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
    12181 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
    12241 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
    12301 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
    12361 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
    12421 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
    12481 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
    12541 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
    12601 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
    12661 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
    12721 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
    12781 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
    12841 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
    12901 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
    12961 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
    13021 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
    13081 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
    13141 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
    13201 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
    13261 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
    13321 caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat
    13381 tctctttaca tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt
    13441 caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat
    13501 cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt
    13561 gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca
    13621 aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac
    13681 tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag
    13741 tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt
    13801 aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca
    13861 gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat
    13921 ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt
    13981 cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag ataaacgttt
    14041 taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga
    14101 aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc
    14161 tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc
    14221 aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt
    14281 ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg
    14341 aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg
    14401 acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac ctatactaca
    14461 atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg acgagaacac
    14521 tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt
    14581 gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcattt
    14641 aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact caggaggaga
    14701 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
    14761 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
    14821 aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag aaaaaccaga
    14881 tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa caaatccaga
    14941 cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag aaaaaccaga
    15001 tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa aaataaacaa
    15061 aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa
    15121 aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc aatcagctga
    15181 aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa taaacagcta
    15241 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
    15301 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
    15361 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac cccaaaatac
    15421 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
    15481 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
    15541 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
    15601 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
    15661 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
    15721 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
    15781 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
    15841 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag agcgacaaca
    15901 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
    15961 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
    16021 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
    16081 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
    16141 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
    16201 tgatgctcgt aaaaattaat aacggactgg atcatgtcaa atgtttgatg aataagcatc
    16261 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
    16321 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
    16381 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
    16441 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
    16501 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
    16561 tgatggaata tgaaaataag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
    16621 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
    16681 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
    16741 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
    16801 ccttgagcag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
    16861 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
    16921 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
    16981 agatactggg tataggtaag attcagcggc ttgaggaggg gagtgtagtg tctaatgatt
    17041 tctttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
    17101 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
    17161 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
    17221 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
    17281 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
    17341 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
    17401 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
    17461 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatgc acttctccag
    17521 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
    17581 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
    17641 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
    17701 atatacaaaa aatatcagag acaaaaaata atctcatcat aaacattaca tatatttctc
    17761 aaaaagttgt tttctttctt caaaaattac ttcacgatgg acctgatgaa gcatccataa
    17821 ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat
    17881 ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga
    17941 gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag
    18001 agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa tgacttcatt
    18061 attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata
    18121 cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc ctttatgcat
    18181 aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat accagctaaa
    18241 gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt
    18301 aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat
    18361 ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac
    18421 ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat
    18481 ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga
    18541 tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga
    18601 taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag aaacaacgaa
    18661 tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag
    18721 gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa
    18781 agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc
    18841 tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt taaaaaaggg
    18901 ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca ctaaaataac
    18961 tatagctcgg tcaaagccta aagcagcttc cagtaatatt cctagaccgc tgacacctcc
    19021 agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata tgaagagggc
    19081 aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt attcacttcc
    19141 ttaattcatt aataatactc ttatcttatt ataacttact tttcacctta agttaacttt
    19201 aattaataaa aaaattaggc tagtctaaat tattatgttt cattcttcta gaatatactt
    19261 ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa
    19321 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
    19381 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
    19441 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
    19501 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
    19561 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
    19621 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
    19681 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
    19741 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
    19801 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
    19861 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
    19921 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
    19981 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
    20041 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
    20101 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
    20161 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
    20221 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
    20281 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
    20341 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
    20401 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
    20461 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
    20521 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
    20581 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
    20641 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
    20701 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
    20761 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
    20821 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
    20881 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
    20941 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
    21001 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
    21061 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
    21121 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
    21181 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
    21241 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
    21301 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
    21361 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
    21421 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
    21481 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
    21541 tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta
    21601 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
    21661 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
    21721 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
    21781 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
    21841 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
    21901 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
    21961 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
    22021 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
    22081 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
    22141 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
    22201 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
    22261 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
    22321 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
    22381 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
    22441 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
    22501 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
    22561 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
    22621 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
    22681 acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc
    22741 attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag
    22801 ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg
    22861 ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat
    22921 tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa
    22981 gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg caattcatca
    23041 ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga
    23101 agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag
    23161 gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga
    23221 tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata
    23281 ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt
    23341 taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat
    23401 gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat
    23461 ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt
    23521 ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc
    23581 aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa
    23641 cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct
    23701 atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag
    23761 caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct
    23821 tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca
    23881 tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct ggttctactg
    23941 atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg
    24001 cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta
    24061 aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg
    24121 attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat
    24181 tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc
    24241 ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga
    24301 aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt
    24361 acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg
    24421 caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac
    24481 tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat
    24541 gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac
    24601 tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt
    24661 tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt
    24721 tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca
    24781 gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct
    24841 ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca
    24901 aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat
    24961 gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg
    25021 acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat
    25081 ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt
    25141 tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt
    25201 atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt
    25261 aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa
    25321 ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta
    25381 tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga
    25441 aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg
    25501 tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat
    25561 gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct
    25621 gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt
    25681 tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt
    25741 caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat
    25801 gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt
    25861 cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa
    25921 aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt
    25981 cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca
    26041 aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta acgcatcttt
    26101 ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt
    26161 gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg
    26221 gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta
    26281 tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc
    26341 ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat
    26401 cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg
    26461 aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag
    26521 tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg
    26581 ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag
    26641 tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa
    26701 agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg
    26761 tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac
    26821 tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg
    26881 tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca
    26941 agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg
    27001 tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg
    27061 aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat
    27121 ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg
    27181 attgtctcta aagcttagtt tctttggctc tttgtcaaat aggactgata gagtgagata
    27241 agaacatcta aggtagaatt ccttttctac tttagatgtt ttttgattca taggtaatca
    27301 gattatttag taagaaaatt ctggtcttca ttttccgaaa gcttcgaaag ccataagcga
    27361 ctcgtttcaa tgctttgatg tgggtatgca gattttctat tttggcattt gaataaggca
    27421 attcgagggc taaacgaatg ccggattcat agtttataaa agcttttttt atagaatgca
    27481 gttcttttgg aacggaattg ggcatcgttc ttacaagttt aaagaaagtg tccgcctctt
    27541 tgtcgtgaaa cgcttgaaga atttcttgat agtaaaaata ggcgattcgt aattcgtcag
    27601 agatcgaaag cagacgatcc accacttcac tttcggtgag atacgcccaa ccaaaattgc
    27661 gcc
//
LOCUS       DACEKQ010000030        27637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEKQ010000030 DACEKQ010000000
VERSION     DACEKQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.031"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            121..768
                     /locus_tag="IW671_001179"
     CDS             121..768
                     /locus_tag="IW671_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0898175.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            852..1796
                     /locus_tag="IW671_001180"
     CDS             852..1796
                     /locus_tag="IW671_001180"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0898176.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1931..3382
                     /locus_tag="IW671_001181"
     CDS             1931..3382
                     /locus_tag="IW671_001181"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0898177.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3403..4239
                     /locus_tag="IW671_001182"
     CDS             3403..4239
                     /locus_tag="IW671_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0898178.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4239..4898
                     /locus_tag="IW671_001183"
     CDS             4239..4898
                     /locus_tag="IW671_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0898179.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5024..5713
                     /locus_tag="IW671_001184"
     CDS             5024..5713
                     /locus_tag="IW671_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898180.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5719..6675
                     /locus_tag="IW671_001185"
     CDS             5719..6675
                     /locus_tag="IW671_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0898181.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6719..7231
                     /locus_tag="IW671_001186"
     CDS             6719..7231
                     /locus_tag="IW671_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0898182.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7487..8035)
                     /locus_tag="IW671_001187"
     CDS             complement(7487..8035)
                     /locus_tag="IW671_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0898183.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8124..9305)
                     /locus_tag="IW671_001188"
     CDS             complement(8124..9305)
                     /locus_tag="IW671_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0898184.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9541..11049)
                     /locus_tag="IW671_001189"
     CDS             complement(9541..11049)
                     /locus_tag="IW671_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0898185.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11208..11399
                     /locus_tag="IW671_001190"
     CDS             11208..11399
                     /locus_tag="IW671_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898186.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11602..12519
                     /locus_tag="IW671_001191"
     CDS             11602..12519
                     /locus_tag="IW671_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0898187.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12512..13585
                     /locus_tag="IW671_001192"
     CDS             12512..13585
                     /locus_tag="IW671_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898188.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13733..14251
                     /locus_tag="IW671_001193"
     CDS             13733..14251
                     /locus_tag="IW671_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898189.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14232..14624
                     /locus_tag="IW671_001194"
     CDS             14232..14624
                     /locus_tag="IW671_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0898190.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14700..15635
                     /locus_tag="IW671_001195"
     CDS             14700..15635
                     /locus_tag="IW671_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0898191.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15768..16547
                     /locus_tag="IW671_001196"
     CDS             15768..16547
                     /locus_tag="IW671_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0898192.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16540..16857
                     /locus_tag="IW671_001197"
     CDS             16540..16857
                     /locus_tag="IW671_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0898193.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17101..18711
                     /locus_tag="IW671_001198"
     CDS             17101..18711
                     /locus_tag="IW671_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0898194.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18868..19716
                     /locus_tag="IW671_001199"
     CDS             18868..19716
                     /locus_tag="IW671_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0898195.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19947..20426
                     /locus_tag="IW671_001200"
     CDS             19947..20426
                     /locus_tag="IW671_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898196.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20459..22687
                     /locus_tag="IW671_001201"
     CDS             20459..22687
                     /locus_tag="IW671_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0898197.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22816..24459
                     /locus_tag="IW671_001202"
     CDS             22816..24459
                     /locus_tag="IW671_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0898198.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKINK"
     gene            complement(24532..25077)
                     /locus_tag="IW671_001203"
     CDS             complement(24532..25077)
                     /locus_tag="IW671_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0898199.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25100..25585)
                     /locus_tag="IW671_001204"
     CDS             complement(25100..25585)
                     /locus_tag="IW671_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0898200.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25575..26387)
                     /locus_tag="IW671_001205"
     CDS             complement(25575..26387)
                     /locus_tag="IW671_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0898201.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26526..>27637)
                     /locus_tag="IW671_001206"
     CDS             complement(26526..>27637)
                     /locus_tag="IW671_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0898202.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9050 a         5027 c         5526 g         8034 t
ORIGIN      
        1 tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa
       61 aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca
      121 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
      181 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
      241 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
      301 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
      361 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
      421 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
      481 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
      541 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
      601 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
      661 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
      721 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
      781 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
      841 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
      901 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
      961 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
     1021 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
     1081 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
     1141 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
     1201 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
     1261 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
     1321 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
     1381 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
     1441 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
     1501 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
     1561 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
     1621 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
     1681 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
     1741 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
     1801 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
     1861 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
     1921 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
     1981 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
     2041 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
     2101 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
     2161 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
     2221 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
     2281 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
     2341 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
     2401 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
     2461 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
     2521 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
     2581 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
     2641 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
     2701 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
     2761 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
     2821 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
     2881 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
     2941 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
     3001 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
     3061 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
     3121 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
     3181 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
     3241 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
     3301 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
     3361 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
     3421 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
     3481 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
     3541 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
     3601 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
     3661 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
     3721 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
     3781 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
     3841 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
     3901 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
     3961 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
     4021 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
     4081 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
     4141 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
     4201 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
     4261 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
     4321 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
     4381 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
     4441 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
     4501 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
     4561 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
     4621 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
     4681 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
     4741 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
     4801 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
     4861 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
     4921 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
     4981 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
     5041 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
     5101 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
     5161 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
     5221 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
     5281 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
     5341 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
     5401 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
     5461 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
     5521 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
     5581 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
     5641 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
     5701 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
     5761 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
     5821 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
     5881 cgaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
     5941 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
     6001 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
     6061 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
     6121 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
     6181 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
     6241 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
     6301 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
     6361 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
     6421 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
     6481 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
     6541 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
     6601 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
     6661 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
     6721 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
     6781 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
     6841 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
     6901 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
     6961 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
     7021 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
     7081 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
     7141 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
     7201 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
     7261 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
     7321 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
     7381 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
     7441 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
     7501 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
     7561 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
     7621 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
     7681 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
     7741 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
     7801 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
     7861 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
     7921 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
     7981 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
     8041 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
     8101 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
     8161 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
     8221 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
     8281 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
     8341 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
     8401 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
     8461 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
     8521 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
     8581 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
     8641 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
     8701 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
     8761 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
     8821 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
     8881 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
     8941 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
     9001 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
     9061 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
     9121 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
     9181 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
     9241 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
     9301 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
     9361 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
     9421 aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa
     9481 aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt
     9541 ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag
     9601 ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc
     9661 ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat
     9721 tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt
     9781 tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc
     9841 gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga
     9901 acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg
     9961 atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac
    10021 gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc
    10081 ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat
    10141 ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt
    10201 cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat
    10261 acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta
    10321 ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc
    10381 ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt
    10441 ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc
    10501 gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat
    10561 atactcattt tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc
    10621 ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac
    10681 tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata
    10741 accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc
    10801 acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc
    10861 tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt
    10921 cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt
    10981 cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat
    11041 ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg
    11101 tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct
    11161 agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac
    11221 agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa
    11281 tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc
    11341 taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa
    11401 aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc
    11461 gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt
    11521 tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat
    11581 gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac
    11641 gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat
    11701 atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa
    11761 aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat
    11821 gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag
    11881 atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca
    11941 acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat
    12001 gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc
    12061 caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact
    12121 gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact
    12181 gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa
    12241 ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag
    12301 acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag
    12361 cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa
    12421 gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct
    12481 aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac
    12541 aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac
    12601 aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga
    12661 aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat
    12721 aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt
    12781 gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc
    12841 agattttcca caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc
    12901 cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa
    12961 tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc
    13021 gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat
    13081 cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga
    13141 aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt
    13201 attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc
    13261 aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt
    13321 gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc
    13381 aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt
    13441 agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat
    13501 ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt
    13561 cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata
    13621 attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta
    13681 tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac
    13741 aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt
    13801 tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt
    13861 aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt
    13921 tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt
    13981 ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt
    14041 ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg
    14101 tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat
    14161 aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc
    14221 aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca
    14281 ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa
    14341 aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag
    14401 aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca
    14461 aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac
    14521 tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat
    14581 tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg
    14641 ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca
    14701 tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc
    14761 tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg
    14821 aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac
    14881 aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt
    14941 tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag
    15001 cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa
    15061 tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca
    15121 aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg
    15181 aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag
    15241 caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc
    15301 tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga
    15361 tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc
    15421 ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag
    15481 ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg
    15541 atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa
    15601 cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa
    15661 aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt
    15721 tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga
    15781 aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac
    15841 cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag
    15901 gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta
    15961 cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata
    16021 cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg
    16081 tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg
    16141 acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg
    16201 ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg
    16261 tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac
    16321 tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc
    16381 aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg
    16441 agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg
    16501 agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta
    16561 cttttagttg tttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat
    16621 tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca
    16681 ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct
    16741 tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg
    16801 atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact
    16861 gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac
    16921 tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg
    16981 ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt gtccttctat
    17041 gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa
    17101 atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa
    17161 gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt
    17221 atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt
    17281 ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga
    17341 gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt
    17401 atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt
    17461 gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc
    17521 ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga
    17581 acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac
    17641 ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct
    17701 ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc
    17761 ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc
    17821 tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc
    17881 gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc
    17941 gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca
    18001 ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg
    18061 atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg
    18121 ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc actatacaca
    18181 ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct
    18241 ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt
    18301 tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga
    18361 ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc
    18421 tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc
    18481 agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc
    18541 gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat
    18601 tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat
    18661 gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa
    18721 tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta
    18781 ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa
    18841 attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca
    18901 tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt
    18961 atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt
    19021 ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt
    19081 ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca
    19141 gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg
    19201 gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc
    19261 ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc
    19321 cgagataata cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga
    19381 cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca
    19441 ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct
    19501 cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac
    19561 gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa
    19621 tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt
    19681 gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc
    19741 aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac
    19801 gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc
    19861 ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa
    19921 aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag
    19981 taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa
    20041 aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca
    20101 aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac
    20161 gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc
    20221 gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga
    20281 accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt
    20341 attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac
    20401 gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga agagaagcat
    20461 ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg
    20521 gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa
    20581 agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg
    20641 gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa
    20701 agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt
    20761 agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact
    20821 tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga
    20881 aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag
    20941 ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga
    21001 gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa
    21061 tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat
    21121 cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat
    21181 gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc
    21241 gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt
    21301 ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta
    21361 ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt
    21421 gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag
    21481 tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc
    21541 tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga
    21601 aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa
    21661 tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca
    21721 aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga
    21781 ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact
    21841 gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt
    21901 tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat
    21961 gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga
    22021 gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg
    22081 tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc
    22141 tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa
    22201 agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg
    22261 agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga
    22321 agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg
    22381 tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa
    22441 actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa
    22501 agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt
    22561 ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt
    22621 tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta
    22681 tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct
    22741 agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta
    22801 aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa
    22861 atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc
    22921 cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc
    22981 tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag
    23041 agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc
    23101 aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa
    23161 tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat
    23221 ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat
    23281 gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa
    23341 agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata
    23401 gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc
    23461 acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat
    23521 ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact
    23581 gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt
    23641 gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa
    23701 cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg
    23761 gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg
    23821 gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt
    23881 tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat
    23941 gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa
    24001 gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact
    24061 tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc
    24121 aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag
    24181 aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat
    24241 cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt
    24301 attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag
    24361 tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta
    24421 gcaccttatg aagctcggtt atataaaata aacaagtaaa acttcctcgt taaagttcat
    24481 tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt
    24541 cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc
    24601 aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga
    24661 tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt
    24721 gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga
    24781 gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa
    24841 atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt
    24901 acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac
    24961 ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa
    25021 tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt
    25081 ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag
    25141 ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag
    25201 ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa
    25261 ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac
    25321 cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc
    25381 ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac
    25441 cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag
    25501 ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat
    25561 agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag
    25621 acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt
    25681 ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca
    25741 gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat
    25801 acctcataat gatcaattga atagagaaga tccacagctt catctccagc catccttgtg
    25861 ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc
    25921 atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta
    25981 ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga
    26041 ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt
    26101 tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca
    26161 attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat
    26221 tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc
    26281 aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct
    26341 ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc
    26401 atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat
    26461 ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta
    26521 aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt
    26581 gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt
    26641 tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata
    26701 atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac
    26761 gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact
    26821 tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc
    26881 caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc
    26941 cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact
    27001 gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca
    27061 cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg
    27121 ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt
    27181 gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct
    27241 ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca
    27301 attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc
    27361 atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact
    27421 ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc
    27481 catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt
    27541 gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca
    27601 gacaaagcag gttgttccat taaataaaga ataggaa
//
LOCUS       DACEKQ010000031        27415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEKQ010000031 DACEKQ010000000
VERSION     DACEKQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.032"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1293)
                     /locus_tag="IW671_001207"
     CDS             complement(106..1293)
                     /locus_tag="IW671_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0898203.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            1615..2457
                     /locus_tag="IW671_001208"
     CDS             1615..2457
                     /locus_tag="IW671_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0898204.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            2454..3404
                     /locus_tag="IW671_001209"
     CDS             2454..3404
                     /locus_tag="IW671_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898205.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            3419..3652
                     /locus_tag="IW671_001210"
     CDS             3419..3652
                     /locus_tag="IW671_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0898206.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            3983..4567
                     /locus_tag="IW671_001211"
     CDS             3983..4567
                     /locus_tag="IW671_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0898207.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            4698..5365
                     /locus_tag="IW671_001212"
                     /pseudo
     CDS             4698..5365
                     /locus_tag="IW671_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            5367..6791
                     /locus_tag="IW671_001213"
     CDS             5367..6791
                     /locus_tag="IW671_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0898208.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            6809..7378
                     /locus_tag="IW671_001214"
     CDS             6809..7378
                     /locus_tag="IW671_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0898209.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            7488..9242
                     /locus_tag="IW671_001215"
     CDS             7488..9242
                     /locus_tag="IW671_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898210.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            9196..10968
                     /locus_tag="IW671_001216"
     CDS             9196..10968
                     /locus_tag="IW671_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898211.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            11491..11760
                     /gene="rpsN"
                     /locus_tag="IW671_001217"
     CDS             11491..11760
                     /gene="rpsN"
                     /locus_tag="IW671_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0898212.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            11775..11954
                     /gene="rpmF"
                     /locus_tag="IW671_001218"
     CDS             11775..11954
                     /gene="rpmF"
                     /locus_tag="IW671_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0898213.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            11986..12363
                     /locus_tag="IW671_001219"
     CDS             11986..12363
                     /locus_tag="IW671_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898214.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            12384..12533
                     /gene="rpmG"
                     /locus_tag="IW671_001220"
     CDS             12384..12533
                     /gene="rpmG"
                     /locus_tag="IW671_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0898215.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            12557..13540
                     /locus_tag="IW671_001221"
     CDS             12557..13540
                     /locus_tag="IW671_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0898216.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            13570..13692
                     /locus_tag="IW671_001222"
     CDS             13570..13692
                     /locus_tag="IW671_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0898217.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            13710..15233
                     /locus_tag="IW671_001223"
     CDS             13710..15233
                     /locus_tag="IW671_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0898218.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            15257..15496
                     /locus_tag="IW671_001224"
     CDS             15257..15496
                     /locus_tag="IW671_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898219.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(15689..16150)
                     /locus_tag="IW671_001225"
     CDS             complement(15689..16150)
                     /locus_tag="IW671_001225"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0898220.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(16345..16563)
                     /locus_tag="IW671_001226"
     CDS             complement(16345..16563)
                     /locus_tag="IW671_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898221.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            16681..18297
                     /locus_tag="IW671_001227"
     CDS             16681..18297
                     /locus_tag="IW671_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0898222.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(18538..19878)
                     /locus_tag="IW671_001228"
                     /pseudo
     CDS             complement(18538..19878)
                     /locus_tag="IW671_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(20154..20309)
                     /locus_tag="IW671_001229"
     CDS             complement(20154..20309)
                     /locus_tag="IW671_001229"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0898223.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(20605..20937)
                     /locus_tag="IW671_001230"
     CDS             complement(20605..20937)
                     /locus_tag="IW671_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0898224.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(21451..21642)
                     /locus_tag="IW671_001231"
     CDS             complement(21451..21642)
                     /locus_tag="IW671_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898225.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(21736..22122)
                     /locus_tag="IW671_001232"
     CDS             complement(21736..22122)
                     /locus_tag="IW671_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0898226.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            22816..24021
                     /locus_tag="IW671_001233"
     CDS             22816..24021
                     /locus_tag="IW671_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0898227.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            24038..24472
                     /locus_tag="IW671_001234"
     CDS             24038..24472
                     /locus_tag="IW671_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898228.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            25089..>27415
                     /gene="esp"
                     /locus_tag="IW671_001235"
     CDS             25089..>27415
                     /gene="esp"
                     /locus_tag="IW671_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0898229.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         9409 a         4019 c         5131 g         8856 t
ORIGIN      
        1 caagtaaaaa ttagccaaat caaaaaatat tttataacag ataattccga aaaaagggta
       61 caacaaataa gcttcttacc attagttttt ttgaatgttc aatagctaat cagaaacaat
      121 tatataaggc tcgtttttag tcaaaatagc atgaatcacg taacaaagct tacgagaaac
      181 cgcacctaag gcagttagat gatgttttcc ttcggaaact tttttctggt aaaacgcact
      241 aaaaacagga tcacattgag aagctactaa agccgccgaa aacaaggctt ttcgcaaata
      301 tggagaccct cgtttactca taacattatg cgttgcctca tattgccctg attgtgtcac
      361 ggaagcgtct atcccagcgt aagcaacgag ttttttcggg ttagaaaatt tttgaatatc
      421 tccgatctca ccaagaatag tcgcaccgtt tataggacca attccaggaa ttgtgagaat
      481 aacagaattt aaagtgtcca ttatgtgctt gatttctctt tccgtttcct ttatttggct
      541 ttcaatgaat tttaattgtt caatcattga acgtagttgg aaggtgaacg cttcttgagc
      601 aaattttact ccaaaggagt tagaagctgc ttgcttcaat tggtccgctt tttctgcctt
      661 gattcggttc ctgcttaact cagctaagta ttgggccaaa gtttctgagg aaatgttttc
      721 taagtcgatc ggggcactaa acgccagcaa taactcttta gaagcttttc cgaaaacgcc
      781 ctgcttggta aaaatcgtcg cgtattctgg aaagacttga tccaatacgg ctataatttt
      841 tcttttgaag tcgctcgctg tacctactaa ataagttcgg taacgagaaa gttgtttcaa
      901 agaaaaaaca ttttcatcgg ataagaccgt ttcgacaaat gagccatagc gcattaagtc
      961 agcaatcaaa acagaatcaa taatatcgtt ttttcgtttc cgtatttctg tacctttacg
     1021 ccatccgtct gtttgaaggg gattcacaac gtgaatcaaa taatctgatt cgtgaaggta
     1081 tgaaaagatg gacagccaat agtgccccgt ggcttccatc cctataacaa agtcactggg
     1141 tgctccggaa tagtcgacca ttcgctcaat taatgactct gctccttcag tggtgttagg
     1201 aaatgaaaaa ccttttagta atacctttcc ttcctcgttc atgatagaag caacgtgagt
     1261 gcgtttaccg atatcaatgc ctaaaaagta catgtatatc tgacctttca aaaaaagaat
     1321 tttggattga aatccactca actgaaaagc gtcactacct tgtacgagat acggagtaaa
     1381 gtaaggactt tcaacatcta actcatacgc aatccactta tcagagagag gagccagtct
     1441 ctcgagtgcg ggttcaatgg cccaaggggg gaacggctta gcctctcaat ccaaaaaata
     1501 tattatctca aaagaaatga gttaatacca aattaaaata acgatggttc atagggttat
     1561 ccaatcaaaa ccggaaggtt aaaaccctaa ctctaatata caagggggaa taatatgctt
     1621 caaaatttta ttaatggtct gtatgacttt cactttttac agaatgctct aattacatct
     1681 gtagttatag ggattgtttc tggaacagta ggttgtttta ttattttaag aggaatgtct
     1741 cttatgggtg atgctatttc tcatgctgtt ttgccaggag tagcaatttc ttatatttta
     1801 ggtattaatt tttttattgg tgcgattgta tttggtctat tagcttctat cattattact
     1861 tttataaaaa gcaatagtgt aataaaagga gatacagcaa ttggtattac tttcagttct
     1921 tttttagcat taggagttat ccttatagga gttgcaaata gctcaacgga tttatttcat
     1981 atcttatttg gaaatatttt agctgtacaa gatattgata agtggattac aattggcgtt
     2041 tctgttttag tgttggttac agttgttcta ttttttaaag aattattaat cacttctttt
     2101 gatccattaa tggcaaaagc aatggggatg aatgtcagtt tttatcatta tctattaatg
     2161 attctattaa ctttagtagc agtaacagct atgcaaagtg tgggaactat attaattgtt
     2221 gcaatgttaa ttacaccagc tgctacagca tatttatatg caaacagctt aaaaactatg
     2281 atatttattt cggcgacagt cggagcgtgc tcatcattgc taggtttgtt cattggttat
     2341 agttttaaca tagcagcagg atcaagtatt gttttaacag cggcattctt atttgtaatt
     2401 agttttctaa tctcaccaaa acaaaaattt ttaagaaaga aggagagaaa gttatgatga
     2461 gaaaatggaa agcagtactg ggaagtctgg ggattttgat tgctcttttt atattcgggg
     2521 catgttcaac aaatagtaaa gacaaagata cagtggcttc gaacgaaaaa ttaaaggtag
     2581 tagttactaa ttcgatttta gcagatatta ctgaaaatat agcaaaagat aaaattgatt
     2641 tacacagtat cgtacctatt gggaaagatc cccacgaata tgaacctttg cctgaagatg
     2701 ttcaaaaaac ttcaaaagca gatttgattt tttataacgg tgttaacttg gaaactggag
     2761 gaaatgcttg gtttacaaaa ttagtaaaaa atgcgaacaa agaggaaaac aaagactatt
     2821 ttgcagcaag tgatggcata gatgttattt acttagaggg tcagagtgag aaagggaagg
     2881 aagatcccca tgcttggtta aatttagaaa acggtattat ttacgctaaa aatattgaaa
     2941 aacagttagc ggaaaaagat cctgataata aaaaattcta taaagaaaat ctagataagt
     3001 atattgaaaa attggattct ctagacaaag aagctaaatc taaatttgct tcaattccga
     3061 atgataaaaa aatgattgtt acaagtgaag gatgctttaa atatttctcg aaagcgtata
     3121 atgtgccttc tgcttacatt tgggaaatca acactgaaga agaaggaaca ccagatcaaa
     3181 taaaacactt agttgaaaaa ttacgcacaa caaaagttcc ctccttattc gtagaaagca
     3241 gtgtggacga tagaccaatg aaaacagtat caaaagatac caatattcct atctattcaa
     3301 cgatttttac tgattcaatt gcagaaaaag gacaagatgg tgatagttac tatgcgatga
     3361 tgaaatggaa cctggataaa attgctgaag gcctttcgaa ataatttgga gggtaggtat
     3421 ggtacaaatt aaaacgacta ctatactaat ttatttacta gcaattcagg aattatcaaa
     3481 aagaaaaaag aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta gtccagcatc
     3541 tgtaagtgag atgctggcta aacttagata tgaggactat ttagatatag gatttcaatt
     3601 aactgataaa gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt aaaagacttt
     3661 atttttaggg aggataagaa aatggctatt ggagtgatta tgataggact taccctaata
     3721 gctggatata atttttttaa atctttctcg gattatttta gaaaaggtga atgatttaga
     3781 tgagtctatt aattcttgta ggattgcttt ttgtatcagt tattttgctc tttctcatta
     3841 gtttagctct aaggaatgag ttcagtaata gacgttaacg attgaaactt caagtcctgt
     3901 gtaagataat atagttaatt catcttatta ggaggaagta cccatagaaa ccagagatta
     3961 tattacaact caccaaaaat taatagaaag tggactaagc cattttgaaa aagaaggatt
     4021 tcagcaaact aagataaggc aactgtgctt gaccgcaggc gtaactacgg gtgcttttta
     4081 taaacatttt acttctaagg aagcgttatt tgaagaaatt attcagccgc atattaatgc
     4141 gcttattaat atgtataatg aagagtttac caaatacctc ttaaacttag aaaatataga
     4201 tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta attatatttt
     4261 tgagaataaa cgtatattcg atttggtttt attccaatca gctggaacca aatatcaaga
     4321 tattgttgaa aaaattacat catttgtgac aaaaaagaca gtctgttgtc tagataagtt
     4381 aaagactatc ggaatgatca atcaaaattt agtctttaat gaagaagaaa tccacttcta
     4441 catttactca ttctacgcta cattctatga tatattgaag catagctatt caaaagaaaa
     4501 gactatgcta ttaaccagaa gattgtatca attttctgtt cctgggtgga ttgaattact
     4561 gagttaatta aaaaaaagct tgcattgaga attgttctca gttatgcttt ttgataagat
     4621 cgcactgtta tctgaataac ggtgcatttt tgtcagctta agataacagt gcgatcttat
     4681 tttttggagg tgaaagagtg tcaaaaatca atccgttaac cttgattact ttaaatattt
     4741 ttttccggtc attatttttt taggaaaggg aaatatattt caagtggggt gtttcattat
     4801 tgctacagtt ttagtattga tgtttcgtaa aataaagaca tgtatttatt tcatcttgat
     4861 ttattcagtt ctgtttttag taatgaaaat tattgatgct atcaagatag agttttttac
     4921 tatatttttt gggacatcct tatatattgt atttcgaatg ttgccggtcg taatgatctc
     4981 atggttgttg gttagtgtct atactagcaa tgaattatta tcctctttag aaaaaataca
     5041 tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc cgacttatcg
     5101 ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct tgtctttttt
     5161 tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg caactatgat
     5221 tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg tgaaaagaag
     5281 ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt ccgttactat
     5341 ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac aatgtttctt
     5401 tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca ataaaaaaag
     5461 gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta ttaaagttaa
     5521 tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt ttatttgaaa
     5581 ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc tacgtaagtc
     5641 aaaatcctag aggacaattc tttacaacaa atacaacgag tgagttagta ttttcgatgg
     5701 aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta gctgaattat
     5761 tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt gaacggcaga
     5821 aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg gatgaaccgt
     5881 cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt gatttaaaaa
     5941 ataaaggatt tactattgtt atagctgaac atcgttttta ctatttgaaa gaattaattg
     6001 atcaagttct atttgttaaa aatagaactg taaaaactac aactattcag ttgttaaaaa
     6061 ctgaaaacaa taacgcatta aggaaatttg acatttttaa tagttcgatt gaagttccct
     6121 atatcgaaaa ggtaagtgca gatgtattaa aagttgaaga aattacttat gaaaatattc
     6181 taaaagacat aaattttgat gtcaaagttg gagatgttat tggtttggta gggaaaaacg
     6241 gtgtggggaa aacaacctta ctaagaatac tcatgaagat tattcgccct accaaaggaa
     6301 agataatcga aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa gaaatggatt
     6361 accaattttt cacagaatca gtgaggagcg agttagccct ggggaatgaa atagttagta
     6421 aagatcagca agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa gatcaattac
     6481 cgtttgattt atccggtgga gaaaaacaaa gattattggt ttctatagct tcattgtcaa
     6541 aaactaattt atttctattt gatgaaccta caagtgggtt ggattatatc aatatggaaa
     6601 ggattgctga ccttattaaa gacttaaaag aaaagggtgc agtagttata gccactcatg
     6661 atccggaatt tctttataaa acatgcaata gaattattta tttaaaaaat ggtggaataa
     6721 aaaaagatat aaaacttaga gtagaagatt caactttgat agaacagatt tttaatgaca
     6781 taacgaaata gaattgagga gaatatatat gaaaacaaaa gattatattt ttattggttt
     6841 agctacagtt atttccctag taatttactt tttttcaata atggttagta gtattggtgg
     6901 ggcgtttggt cacagcatat ccccagggat ttttggatta ttatctggaa tcatatttgt
     6961 ttatattagc tataactacc ctagaaaagg aatttttact atttacacaa ttgttttatt
     7021 gttatttttt acgttaatgg gcggggcgta cttaccttgg tttatcagtt cgattagtat
     7081 ggcgatctta gctgatatta ttctatctgt atttggatat gatcgagcaa ttcctcaagt
     7141 tgtgtcttgg gttctaatgc aactaggtag tgctgccgga cagtggattc ccatatggtt
     7201 ctttacagat cgttttcgtc aagactggat agatagagga caatctgctg caacaatgga
     7261 tgcaatgatc cactacgcgg tgggtatatg gggaattatt tcagtgttag ttgtagcaag
     7321 tttatctatg attggtgtat tgattggaag aaaagtctta aaaaaataca aaaaatagaa
     7381 cgtagtttac cttctatctg tttgtacatc tctaatttac gctattacgg aaagcattaa
     7441 cagttgtaaa cgaataagag aagaaattga atgcggggga ggaaaaaatg aaagtataca
     7501 aaaaattatt tagttatgtc cctaaaatga aatactacgg atggttagcc actttatttt
     7561 ctgatttttc tgtcgtatta acagtctatg gatattacag tatttacaaa tttcttcagt
     7621 ctctaattat tgtaggagat gaatatttag ctaagagtta tgcggttcaa actgttgctt
     7681 ggttaacttt aggagcttta ttttatattt tttctggctt attttctcat atccttgggt
     7741 ttcgcttaga gactaatttg agaaaaaagg gaattagcgg tttaacagaa gcaagttttc
     7801 gtttttttga tttaaattct tcgggatata ttcgaaagac aattgatgac aacgcggcta
     7861 aaacacatat ggcaattgct cacttgattc cagatagtgc tcaagccata gtaacacctg
     7921 ttttatcagt tgccttaggc tttttcatta actttaaaat tggattggtt ctaatcgcca
     7981 tgttagtaat tggtgtattc ttgttaaaga aaatgacaga gaatacaaat tttattcaaa
     8041 aatatcaaga atcgttagat gtgttaagct ctgaaacggt agaatatatc cgcggaattc
     8101 aagttataaa gatatttggt gctaaagtaa catcatttaa agccttgaat aaagcaatta
     8161 acgattatgc taagtttgca tataagtatt ctttgagtgg gaaaacacct tttgttcttt
     8221 ttcaatggct attttttggc atagtttgca ttttgatagt tcctataacc ttttttataa
     8281 cttctcttac cactcccaag atcttagctg ttgacttgtt gatgttgttt tttttaagtg
     8341 gaattatttt tgtttctttc atgagaataa tgtatgtaag tatgcacttg tttgaagcaa
     8401 gatatgcagt cgaaacacta gactcccttt atttagaaat gaagcaagac caactaaaat
     8461 atgggaacga gacacagatt aaaaattgtg acattgcatt tgaaaatgtc agcttttcat
     8521 acaaagaaga gtgtgtttta gaaaatttca acttagtttt agaacaaaaa agaatttatg
     8581 cattagtagg aaaatcagga agcggaaaga gtacgatagc taaactaatt tcaggtttct
     8641 acaatgtgga taagggacag attaaaattg gtggtaagcc tttaaatcac tattcaaaag
     8701 acacaattat taacacaatt gcttttgtct ttcaagatac gaaactattc aatcagagta
     8761 tttacgataa cgtttctata gcaaatcctc aagcgccagc aagtaaagta tttgacgcgc
     8821 taagattagc cggttgcgaa tcgattttag aaaaatttcc cgaaagagag cataccttaa
     8881 ttggtagtaa aggagtttat ctgtcaggag gagaaaaaca acgtatagct attgccagag
     8941 caattttaaa agatgcaaag attgttatta tggatgaagc cagtgcatct attgatccag
     9001 ataatgagta tgaactacaa aaggcttttc aatatttgat gaaagaaaaa acagttatta
     9061 tgatagctca tcgattatcc agcattaaat ctgtagatga aattcttgtc ttgtcagaag
     9121 gaaaaatagt agaacgtgga aatcataatg aattaatggc taaacatcga gtgtataaac
     9181 aattggtcac taattatgag aatgcaaatg attggaggct tgacaatgaa aaatttttat
     9241 aaaaaaaaat ttgctttaac tgatcagggc gcagaagctt taactaaagc ttctatttct
     9301 agtttttttg tatactgcat taatatggta ccagcattca ttataatgat gctgatagac
     9361 gaattagttt tagaaaatgc aaagccccgt tggctttatt ttgcagtatc gtttgttact
     9421 ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa tagtacttat
     9481 aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt accattatcc
     9541 tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga cgttgaaggt
     9601 attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct gtttttccct
     9661 tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt tattttgcca
     9721 acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa agccaatact
     9781 aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat tgaattgcag
     9841 caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt caaaaaaatg
     9901 gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt catggcctta
     9961 tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt tcacttactg
    10021 ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc aataaaaata
    10081 aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt agctcctaaa
    10141 atacagcgaa ttagagctat gaaggaaacc agtatccaag agggatcaga tagtcctctg
    10201 aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa taccccaatt
    10261 ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt
    10321 ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta tgatgaaggc
    10381 tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa
    10441 atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga aaatatacgt
    10501 attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt agctaactgc
    10561 gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga aaacggaagt
    10621 tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt gaaaaatgcg
    10681 ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga aaaacgtatt
    10741 caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc gcatcgcttg
    10801 aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact agttgatcaa
    10861 ggtactcata atgagttgta tcgaaggtca gaaatataca aaaatctcat aaagaaaact
    10921 aaactatctg agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg aaataggctt
    10981 taaattaacc tacaattttt gggatatagt tataattctt tttcaaaaag aataatttta
    11041 ttaaacagca tctaattttg aatacacgaa agaatcagta tgattgaatt agttcactta
    11101 atgtctattc ttataatata aggaataatc aaagttttaa aaaattggcg ataaaccagt
    11161 taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt ttaagtagag
    11221 gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga attcttattt
    11281 ttcagagcct aaactgcata ttcttttaaa tgatttgatt atctctatgt accgataaat
    11341 tcgttttcaa atagttatct attcatatga gttttatttc atgttattta ttgttgacaa
    11401 gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc
    11461 taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca
    11521 aagaaacaaa tggcgatgat tgaaaagtat gcagataaac gtcaggaatt aaaagccgca
    11581 ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg tctgaggctt
    11641 cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat gtcccgtatt
    11701 aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc aagttggtaa
    11761 aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa
    11821 gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt
    11881 atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa gtaattaaag
    11941 gagcttcaaa atagtttaat atataacttt taggagggaa tcaaaatgga aattatttac
    12001 ccacctttag ttgaagaagg attaaagtat tatcttgaaa cgacccagca atcgctagat
    12061 aaatgcactt tttatcgttc gatggtagag agaggaatta ttactgaaac gggtttaccg
    12121 acccaacagg caattgaaaa tggattagtc aaagattact atgaggacca aggcttatca
    12181 tttgatgatt ttttaagaat ctatccaatt tttgaagagt atgacgagga gttatttcaa
    12241 tgtatcgatg gttattggga gatacctgtt gatatgaaag aaaatttagt ttcacaattg
    12301 gaatctggcg agttaacttt tgaagatgca caacaaattc aagcatatct tgaagatcga
    12361 taagtattga aggaggaaga tttatgcgtc aaaatattat tttggaatgt gtcgaaactg
    12421 gagaacgtct gtatctcacc agcaaaaata aacggaataa ccctgaacgg ttagaattaa
    12481 aaaagtattc gccaaaattg cgcagacggg caatttttaa agaagtgaaa tagctaagtg
    12541 gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt tctgggtttt taggggctgg
    12601 aaaaacaacg ctaatcaatc aagccttgca agtctcaccc taccccaggg aagaaattat
    12661 tataattgaa aatgagtttg gtgaagttgg gattgatcac aaattattag ttcatagcga
    12721 ggaacgggtg ttacaactaa ataatggatg tatgtgttgc agtcttcgca gtgatttatt
    12781 agcaatattg atttctttac tggaaacaat agaagaacat caacaaccaa tttcacaaat
    12841 catcattgaa actacaggta tagcagatcc ccaaccaatc attcaaacac ttttaacagc
    12901 gcctcaacta agaggaagag tatatatcga tagtttattg actgttattg atagtgacag
    12961 ttttgaactt attgaaagag aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat
    13021 ttttgtgtct agaaaacaaa acttgaattt atctgcacta aaaaaattac agagagaaat
    13081 caaaagtatt aaccctttat ccgatattcg aatgttcaca catagtgaaa cgattcaaaa
    13141 aaaggatttt tataatttga aaaaatttaa tcatccctta gtttttgaaa gtgattcatc
    13201 agaaaatcac tataatggca aatactatca tcaacaccat caagtacatg agtttaaatc
    13261 aattctctta acttcagatc aagacataaa aaaagatttt ttactccaat ggattgactg
    13321 gctgatttta accaaccaag ggagcctgta tcgttttaaa ggattgattg ctttacaaga
    13381 gcaggatttt atagttgcca tgcaagaggt aaacgaacag gttaattttc aatacaccac
    13441 tcgacaaaaa gaccaaacca ctattattct aatcggaaaa atgttggatg aagaaaggat
    13501 caaacagagt tttgataaat tactggcgga aagcaaataa tgataagtag tgattggggg
    13561 taatttctaa tggagaaaac ggatatatct agtgcatatc gacgacttaa aagcccgaat
    13621 attaaaacta gaaaaagggc attaaaaatt atcaaagaac ataaacgaaa taaaatgaag
    13681 aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat cagtgtagga cttattggga
    13741 tagcagctct aggattattg ggagcttgct ccagcactaa tgatgcaaaa gtatcgaatg
    13801 ataaagatgg aaaattagaa attgtaacaa cattctatcc aatgtatgat ttcaccaaaa
    13861 acatcgtcgg cgatgaagcg aacgtggatt taatggttcc agcgggatca gaacctcatg
    13921 actatgagcc ctcagctaag gatatggcaa aagctcatga tgctgatgtt tttgtatatc
    13981 ataacgaaaa catggaatca tgggtaccaa aagctaaaga aagttggaag aaagctgggc
    14041 ctaatgtagt agaaggaaca aaagacatga ttttattgcc aggtagtgaa gaagaagatc
    14101 atgatcacgg agaagaagat caccatcatg aactagatcc tcatacgtgg gtttcaccca
    14161 aaatggcaat taaggaagtt tcaaatatca aggatcagtt agttaaattg tatccgaaaa
    14221 aagcaaaagt atttgagaca aatgctgaaa agtatttgac taaactgaaa cggttggatg
    14281 ctgactatac cactagtctt aaagaagcga aacaaaaaag ttttgtgacg caacacgcag
    14341 cctttggata tttagctttg gattatggat tgaaacaagt tcctattgct ggactaagtc
    14401 ctgaagaaga gccatcttct ggtcgattag ctgaattaaa agagtatgtt aaaaaaaata
    14461 agatcaatta tatttacttt gaaaagaatg ctaatgataa aattgcaaaa actttggcca
    14521 acgaagctgg gataaaattg gaagtactga atcctcttga aagtcttaca aaagaacaaa
    14581 tggataatgg tgaagattat gtttcagtaa tggaagacaa tctaaaagcg ttagaaaaaa
    14641 caactatggt tgctggtgaa gaggtagttc cagaaaaaga agcgaaggat gaaaagacag
    14701 ttgctaatgg gtactttaaa gatgccgatg tgaaggatcg cgaattgagt gattatacag
    14761 gcgaatggca atctgtttat ccgttgttaa aagatggaat tttggatgaa gttttcgatt
    14821 acaaagcaaa aatcaataaa gacatgacag ctgccgagta caaagattat tatactactg
    14881 gctataagac agatattgat actatcaaca ttaaagacaa caccattgat tttgtagtca
    14941 acggtgaaca tcatcaatac acatacaaat atgttggata caaaattttg aattatgaaa
    15001 aaggaaaccg tggtgttcgt tttaattttg aaactgatga tgcaggtgct ggtcgtttta
    15061 aatatattca atttagtgat catggaattg caccaagtaa ggcagagcat ttccatattt
    15121 tctttggtgg agaaagtcaa gaaaaactct ataatgaatt gcataattgg cctactttct
    15181 atccagcttc attgtctaaa catgaaattg ctcaagaaat gatggctcat taaaattcaa
    15241 tttttggagg aaatacatgg taagaaaagt attattgtct atggattcca agaatattga
    15301 tgcttatcgg cgttatcgat ttgcaaagca aatgagatta aatgggagta cattagctga
    15361 tattgctgca actttacatg ttagtattga cacagttcat agagtattaa aacgggatcc
    15421 tgattgttgt tttatcaagg tcaatactgg aagaagtaga cgtttagcaa aacggcataa
    15481 tagcttggcc aattaattga taataccaga gtagatcatt tttcccttat ttggatttga
    15541 tctgcttttt attatgtctt gaattgtcaa atcaagcgca acactttatc aaagaagact
    15601 tcataaggtg ttctccaatc taaacacttt ctaggtctgt tattcagttt ttcaatcatc
    15661 tgatttatcg ttgagttatc taccgtagct atctctttcg attttggcaa gtattctcga
    15721 atgagtctat tcgtatttta atttgttcat tgttgccaag ggcatgagga tctggaaaat
    15781 aaaagggcat attattcaat gcttctgtaa ctgcgaaatg attggagaat tcttttcctc
    15841 tatctggagt gatcgttttg accgcctcat ttggaatact gcttaaaagt tcaatcattt
    15901 tctcgctcac ataagcggct ttctttctgg ggaccttctt tcctgctaaa aggaagcatg
    15961 attttctgtc tgtcaaagtc actaaacaag taccaccagt tttgccagct acaatatcgg
    16021 ctttccaatg accaaaatca cttctatcat ctgcggattt tggacggcca tggatcgaat
    16081 gagtaaggca aatctttcca cgagactcga catgattttt tgtatgtctt gtttttcctc
    16141 gatggtgcaa tgaacggatc aggcctttat tgccgtgtga gaggggttct gtatcgaaaa
    16201 gatcattgca aattgcacgt tagatcgtga taaaactaat tgaaaagtcc atatgttcat
    16261 gcttcaaacg attcgagatt tcctctggcg accactgaaa atcgacaaat aaatgacgaa
    16321 caattgacca aactcacgga tcatttagaa tgtgataacg tccgcatttt tttcttcgat
    16381 aagcataatt tttttccgct ttggaaggtg aatagttttt ttcttgtttc ttctcagttc
    16441 atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca attatactaa tagaaaatcc
    16501 ttcttcgtga agcagaaaga ttctttctcg ttcgagtata gtaagatgac gatactggct
    16561 cataattatt ttccttctag gagtgttctc gacaaactta tactagttgg atatcgatta
    16621 tgactcattt ttatgtgttg cacttaaagt gtaaattcaa ggtatagaaa gaggttccct
    16681 atgaaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa aacgatactt
    16741 ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt aaatgacata
    16801 ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag aactggagaa
    16861 aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga tttgattgtt
    16921 gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga agcttcagga
    16981 atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc tttagacatt
    17041 gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt aaaacgtttt
    17101 tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg taatgtagtt
    17161 gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg taacatatta
    17221 cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa cggtctaatt
    17281 gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt agacttatcg
    17341 aaaattctaa atacaaatct ttttaatata gatgagactt cctattcagt gggctggttc
    17401 aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata tggtatctca
    17461 agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca tcttacggat
    17521 aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc ccaacatagt
    17581 gatttttcga cgatctttga aacatcgggt agctataaaa gtattgaatc attaaattat
    17641 tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa tcccgaattg
    17701 aaggaagcct ggcatccgtt gtttggtgaa cgcattaata aactagtttt tattggtatg
    17761 aagatggatc aacaatgtat taaggaacaa cttgagggtt gtttaactga ttctcttaat
    17821 gcagataatt tccgaaaaat ttcaccattt ccatggaaaa agtctaatat gtttagccag
    17881 atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt cataattatt
    17941 ggcattttga ttattacctc ttttattccc ttatttgtat tctctataga aaaggagaat
    18001 gaaacaactg aagatgctat caaatatgcc atcgttcgaa ttgatggcaa ggaaatggat
    18061 cgcttcgatt tggataagat caatcataaa ctcgtgaatt atcatcctgc gaagggacaa
    18121 tataatattg ttgagattaa agatggaaga attcgagtta aagaagataa tagtcctgat
    18181 caaatcgcgg ttagaactgg atggatctca aaacccggtc aaacgagtat ttgtttaccc
    18241 cataagttag ttattagtat tgagaaaaaa gaaagcacag attactacat ttactaatct
    18301 ggaaatagat gaggaggttt ttaataagaa aaagatatta ttattaataa tagcaggggc
    18361 tttattaagt gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa caggcacaag
    18421 tatctttaac gggacctgca atggtttagt taacagtcgg ttagattact tattttggct
    18481 cttcgtcaat gagtgttggt gaagaaattc aatagaataa tcgagatact cacacggcta
    18541 gacagctatc ttgtctagcc gtgttgttgt tctggcttta gtcagttggg tagtattctt
    18601 agttttctta aaatattttc ctcgatataa aaagattcgt tctcggtagt tttgaaagtt
    18661 tctatagcca taggcaattc gttttagtac tttgatatgg ttattcaaac attctatagg
    18721 gccgttggaa tagggaacgc gcaacgctcg tttgatttct gtttggtatt tcttgaaggt
    18781 cgtgaacact ggttggtagt gctctggcaa ctccttgtag tcttctttta ggagcgcgac
    18841 gaattcagga acatcttgtc ttctgataac ggaaagaaaa tcctggtaga cttcgtagcc
    18901 tcgctttaag gaatcatcat aagataatag ccgatcgact atctctgctt ctgtcagatg
    18961 atctcgaaat gacggacgcc agtagcgatg ctgacctttc aattcccacg cattcttttg
    19021 aagtagtttc cagtatttct ttaagtgctt cgaccgacga tccccttttt taaaggtctt
    19081 cattacttga attcggtggt tggtaaaagc ccgaccaatg tgttggctaa tatgaaagcg
    19141 atcggcgacg attcgggcat tcggaaaaca ctctttcacc aatgtgcgat agggtgtata
    19201 gaaatctgaa accactagac gcacttgttc acggactttt atagggtagc gcaagaaata
    19261 gtcgcgtaaa ggattgagtt gccgattttc cacaatatca atcagttgat gggtcttgcc
    19321 gtccatcatg acaaagctca tggctcccga gacatttttc actgatttga attcatcgaa
    19381 acacaccact tctggtaaca gctggctcaa agaaatggtt ttaggccgaa ggctgcgaag
    19441 tattcgaatg acagtggtcg gagaaatgtg cttcatacgt gcgatcaaag acatagaaag
    19501 aggttcgctt aatagctcaa gaatggcttg tttgactctg cgagcaatgg agcagcgacg
    19561 atcactaact gaatcttctg ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg
    19621 tttgttaatc cgcagaatgg tcttgtagtt actgacatca ttcaataaga tggatacttt
    19681 tttccagccc tatttaatga tcgtttgttc attttgatag tggcgatgaa agcattcctc
    19741 cggttgatag gtcaatgtgc catcaaaagc aaaacatgtt tcgtcattga tgcgctcttt
    19801 gcgaaaggca tcctcatgaa aggtaagatt taggtctaga atatctagta actctttggt
    19861 aaactctgaa taggacatct tgaaacacta ctttgaatgg tttttgtcga ctttattcta
    19921 aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa
    19981 ggcgtcacca acactagata ttatagatcc cttattttct tcgcttcaaa ttgtcaagga
    20041 gtcatcataa attttgtatg gattcttttg tttgggtagt agtcttctaa ataatcttgg
    20101 attaacacct tcatctctat tttactttta tatttgttgg agtcgattaa ttctcatttg
    20161 attgatcgat gaaacgcttc aattactgca ttgtcaacag gagtcccagt ttggctcact
    20221 gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa taaaagaccg atgctggcta
    20281 cctctatcgg aatgaattac cgtaggcaat tctccttaat aaatttgtaa tggacggacg
    20341 acaatctctt tttttatgtg tgaatctacc atatagtaac gtagaatacg agttgctaag
    20401 tcaatatagg tggacaaata gaggcgaccg tcattacatg gaatgtaagt aatatccatc
    20461 acccaaacag catcaatttg gttctgttca aattatcgat tcaacatttt tttaccattt
    20521 ctttgattgt tttttctcta ccatgagaag atttgcgtcg atgaaatcct cttgtgcata
    20581 aattcatttc acgcataaga acagctacaa cacgtttatt gatttcgatt ccttcatcgt
    20641 aaagtttttg agtgatccct ggagatccgt atcgatcttt gtgttcgtag aacactcgtc
    20701 ttcctctttt agtcgtttat tttcctgttc aagaatgtag aaatctgctt tatcaggtaa
    20761 tctttttcct tgtccagcaa aagcattttt cccgtatcga cgatattctg aaacccatcg
    20821 ggaaattgtt ggacgagaga tattgtattc ttcagcaatt ttcttggtag ttactttttt
    20881 ctcaaggata tcctgtgaaa tctttagttt aaatttacga tcatatgttt ttctcaatag
    20941 agttcattcc ttccggtaac gtaactgata gtcaagaaaa gtgtatcata gcccaactta
    21001 gtatcagggt ttgtcaacta aactgtggaa gttgatagat aagagttttt aacaaccaaa
    21061 attctcttga ctatttttga aatcagacaa attttgattg gacacatagt tgaataaatc
    21121 taccgtcgtt acggaaggta aatcgcatgc ttttcaattc tagaggagaa gtcctcgttg
    21181 aagtaccata gcccagttct ggattcgacc tcctgcccac ttcatacgta aataaagtaa
    21241 ttttaggagt gagactgtga caaaagttat ttttaagcta aaaacgaatt aaaaaacgtt
    21301 gataattaaa atcattcgtt ttagattcta tgaatcatta tagaacgctg taaaatcgat
    21361 actttataat tataaagaag atagtttcaa atttattgtt cgaaatttga aactatcttt
    21421 ttacttatgt cacagactcc cttagttgag ttattcttta atttcgaatt tcgagatagc
    21481 tagatatatt gcaagcatac attcttgata acaatcttga tcatattttc cagttctcca
    21541 ggattgctta tgaatcaaag agctaaatct tttgagaatt gcctcaacag cttcttcatt
    21601 tccagccttt gctaaaatga aatcttcttt taaagaattc ataaatctac cttcttccca
    21661 tggacttata atctatttct ttgggaagga ggagaacgag caaagaagca tacaaaatta
    21721 gttattatgc agatattaaa ccagcatttt ttgaattttt ttgtaaagtc gatgttttaa
    21781 atttgtcgca ccctgtcttg taatccccag aacctttcca atttctttat cagttttatc
    21841 aaagataaat ttttcgataa gaaaaatttg ttcaggttct ttcaacaaag gaaggatttt
    21901 tgctaatttt atatcttcaa caaggagagt cacatttaaa acattaatcg gttgtttaaa
    21961 ttttatagta tctttagtaa acaaatatgg tgggagttga tcaagttgtt catgtttttg
    22021 aatttcttct tgttttttat aataagtatt agcggtgttc aaaatgacct tttcaaagaa
    22081 tcgaatcaac tgggcattta cgcgttcgtc gttatctgac atattgcaca cctcctccat
    22141 taagatttaa agggaaaaga ggcaaaaaac acgaaaagaa gctaatgcaa ttatgctgcg
    22201 ctgcgaataa cttctataag ctcacatgca ttgataggac tgctgatgtt tattttcaaa
    22261 aatgtttttt tagttgataa tggatagtta tctttatact tagaaaagaa aattcatata
    22321 tcttcttaaa tatcaaacat gtccttatgg ctaagaaaat gtcttgagct ttttacgtat
    22381 aataaatctt ttctaaaacc tccttttgga taatattctt attcgtgtat agattgtatt
    22441 ttttatttct tcccccttca taattaacaa ctagaaaaga gttcgaatag acacgtattt
    22501 gaacgaaaat ttttattgga ttcccaaatt ggaatattta gaaacgaaaa attcctatta
    22561 tctgtatagt gcttagataa taacggatca taataattat tgtctttgaa ctagatatat
    22621 gtaaatagta ttcatcatga tgaaaaatga tgagttctcg gtgataaaat gtacttttta
    22681 cggtggaata tacatgaaag cgataagtgg atattttata atgaagtaag ttagcttatt
    22741 ttgacagagg aatagtattt tagataacgt ataaaatact atttaatgat gtagaaagga
    22801 aagagagaaa caaaagtgtt ttcagataaa gaagtcttat taaagtctct tcatgtttgt
    22861 ttaaagttac ctatcgtggt ttttaattcc gaattcagta ttataaaaga gtatcgctca
    22921 gatagaacta cttatttttt ttatgattat aaaaagttac taactgataa tattaaaaaa
    22981 actaaagatt tccatttctt tactggtgat tttaatgaga tgtttcttct atacatgttt
    23041 aaaaccagat attacttatt tggtccattt agagcaaata atattgataa agtttttttt
    23101 aaactaaaaa tgaacaattt gaatgttgca atggctgata gagaacgact ctataattca
    23161 ttgcaaaatt tgactttgta ttctttagga gacattcgag atattctgat tttagttcat
    23221 tattttttta ctggaaaaat agaagatcta ttccatgaac cgttaattga atatacagaa
    23281 aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat
    23341 ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat
    23401 attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt
    23461 ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa
    23521 acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata
    23581 caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt
    23641 gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct
    23701 tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct
    23761 aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt
    23821 acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca
    23881 aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg
    23941 agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac
    24001 aatttcaaca acattagctg agaggtttta ttttattatg agactatatc atttacgtag
    24061 tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt
    24121 gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata
    24181 tgacaaagta gtaattaata aagtaaaaag tttatgtgaa gaagctagag aatctatcag
    24241 tcatattaat ggtagcaatt ttaaacaaac attatttaat ctgattaagc tagatgctaa
    24301 aatatcaagt tatttattct ttttgataca tgatgaattt atggattatc aaaaattgga
    24361 tcagttaatt gaaagagaaa gctgggaaga ttattacgtg ggtctaactt tttcagaaaa
    24421 gttaaacaac tacaagttaa tagattataa gtatttgagt gaaagcaatt aattattcat
    24481 gtagagtact agacacttta aatatggaca attcaattat aaagaaaaag aatcaataat
    24541 ttaatgaggc tgtgacaaat gtcatttttt gtttcgctaa aaacgagtga aaaaatgttg
    24601 ataactccaa tcattcgttt aagattctag gaatcattca gaaatgctgt gaaaccgata
    24661 ctttataatt ataaagaagg tgatttcaaa ttccgaagaa ggaatttgaa gtcacctttt
    24721 tacttatgtc acagtctcat tcagtaatgc gggataaggc tccttattcc gttatttgaa
    24781 tactctgaag tttaatctaa gaaagggtaa tattttgaaa acatgcttca tcgcatagaa
    24841 atgaacttaa tttcagaaag aatacataca tatcttttta tatgttttca gctgctgaat
    24901 ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat gtactttttt tgttgtaatg
    24961 ttcatgaaat tctctaagta aattagttaa cataaggtta gtaagattcc tattctgtga
    25021 ataatacgta tcagatttat cttttattct tactaggaaa gtaattatta agggagttga
    25081 tttgaataat ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag
    25141 tattgaagta cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg
    25201 gtcttgtctt gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc
    25261 cacttgaaac agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt
    25321 ttcaggagaa tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc
    25381 aagaactaac tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac
    25441 ataatattgc agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag
    25501 ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga
    25561 cctttgtaag aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata
    25621 ttcgtgcaag tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta
    25681 atggggtaat tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca
    25741 atctcaagac cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata
    25801 aaattgtacc gggtattgat agtacgggct actttttaac agattcagat gatttctata
    25861 atagcttaga tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa
    25921 aatatgagaa acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag
    25981 ctataatctt agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa
    26041 agggcacgcc ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg
    26101 gtatacagat gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg
    26161 gaacaaaagt agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag
    26221 gttcaataac taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc
    26281 cagtcgttat tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata
    26341 tgaaagcaac agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt
    26401 cttggttgtc ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt
    26461 atttgcaaga tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga
    26521 atccatatat cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg
    26581 ggaaagtatt catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag
    26641 aaggtactgt attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt
    26701 cagatactgt agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg
    26761 aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta
    26821 ttaaggaatc accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg
    26881 ctaaagaatc aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa
    26941 cagagcctga tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg
    27001 gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa
    27061 atgatgcaac atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac
    27121 ctacatttga taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct
    27181 ataataagga cttcggtaat gcaagtgttg atgcaaacac tggtattgtt acattcactc
    27241 cagctaaagg tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt
    27301 accaagatgg ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg
    27361 aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc gcagg
//
LOCUS       DACEKQ010000032        26902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEKQ010000032 DACEKQ010000000
VERSION     DACEKQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.034"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..1146)
                     /locus_tag="IW671_001236"
     CDS             complement(40..1146)
                     /locus_tag="IW671_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0898230.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="IW671_001237"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="IW671_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0898231.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="IW671_001238"
     CDS             complement(2694..5039)
                     /locus_tag="IW671_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0898232.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="IW671_001239"
     CDS             5318..6220
                     /locus_tag="IW671_001239"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0898233.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6433..8208
                     /locus_tag="IW671_001240"
     CDS             6433..8208
                     /locus_tag="IW671_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0898234.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8274..9860)
                     /locus_tag="IW671_001241"
     CDS             complement(8274..9860)
                     /locus_tag="IW671_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0898235.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            10111..10335
                     /locus_tag="IW671_001242"
     CDS             10111..10335
                     /locus_tag="IW671_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0898236.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="IW671_001243"
     CDS             10476..12227
                     /locus_tag="IW671_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898237.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="IW671_001244"
     CDS             12227..14011
                     /locus_tag="IW671_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898238.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /locus_tag="IW671_001245"
     CDS             14337..15188
                     /locus_tag="IW671_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898239.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15285..17165
                     /locus_tag="IW671_001246"
     CDS             15285..17165
                     /locus_tag="IW671_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898240.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17205..18107)
                     /locus_tag="IW671_001247"
     CDS             complement(17205..18107)
                     /locus_tag="IW671_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0898241.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18338..19741
                     /locus_tag="IW671_001248"
     CDS             18338..19741
                     /locus_tag="IW671_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0898242.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19741..21174
                     /gene="ascB"
                     /locus_tag="IW671_001249"
     CDS             19741..21174
                     /gene="ascB"
                     /locus_tag="IW671_001249"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0898243.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21319..22245
                     /gene="trxB"
                     /locus_tag="IW671_001250"
     CDS             21319..22245
                     /gene="trxB"
                     /locus_tag="IW671_001250"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0898244.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22459..24186
                     /locus_tag="IW671_001251"
     CDS             22459..24186
                     /locus_tag="IW671_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0898245.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24258..24551
                     /locus_tag="IW671_001252"
     CDS             24258..24551
                     /locus_tag="IW671_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0898246.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24643..25503)
                     /locus_tag="IW671_001253"
     CDS             complement(24643..25503)
                     /locus_tag="IW671_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0898247.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25595..26023
                     /locus_tag="IW671_001254"
     CDS             25595..26023
                     /locus_tag="IW671_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0898248.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26185..26361
                     /locus_tag="IW671_001255"
     CDS             26185..26361
                     /locus_tag="IW671_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0898249.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26388..26834
                     /locus_tag="IW671_001256"
     CDS             26388..26834
                     /locus_tag="IW671_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0898250.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8515 a         4797 c         5746 g         7844 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagtacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccgc ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aatgggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agtcatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaataaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agccgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagatcg tggcgtgaaa gtaccggaag
     2161 gattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatctc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttccag gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatt gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaacg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaagg agttaaaacg tgagaaaatg
     5161 atctttttta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccgaca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc
     6781 gtgaagaaca agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttact agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct accaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     8041 gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt
     8101 ttaagtgtct acactggcgc aggcgtgccg ttaacgcaat tcatcgtagt aacaagcatg
     8161 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8221 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8281 atttcttcta ccatttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
     8341 ccaatcgtcc atgccaacag gaggaggaca agaacaatga ttacataagc gatgtgatca
     8401 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8461 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8521 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8581 gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8641 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8701 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8761 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8821 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8881 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8941 gaaggatctt gaacagaacc ggttttgaag acagcgacac ccataatcaa tagcagcgca
     9001 ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa acgtacagct
     9061 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgtgaaac tgctgaatgt
     9121 agatataagt tatgtggcat gactgttgcg ccaataatcc ctaatgctcc agttagcgga
     9181 gtttcgccac caatgacagg tgagtcggag aatgtatggc tgttagggat aaagccagca
     9241 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9301 acgaaaatca agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9361 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9421 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9481 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc tcggattgct
     9541 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg taaaagcata
     9601 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9661 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9721 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc cactgttcca
     9781 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9841 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9901 cacctcaaaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt tttcgtggcg
     9961 tgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
    10021 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10081 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10141 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10201 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10261 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10321 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10381 ttttatttcg taatttgtta gactattcag attattcagc aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aagttaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag attgccgata
    10621 aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcatttcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt
    12781 gccaatttta ggaatagtca tctggtatta ccagaaattt agttctcgta tttaccggaa
    12841 tatgcgggaa aaattaagtc agctgaatac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacaaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac agaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtagc attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga gagttagtgt
    14101 cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta atagccccta
    14161 agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctaat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga gatcgggaaa
    14401 tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg attcttgatt
    14461 catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag ttccaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg gatcacttgt
    14641 atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg ctgtatgata
    14701 tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca acaatacagg
    14881 atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccact
    14941 tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgttctagat ggagcgaaat
    15001 cagaagatgg agaagtttct gaggaattgt tccaagttgt tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac taccagatgt
    15121 ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaaataag ggtagtgaca
    15241 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    15301 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    15361 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    15421 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    15481 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    15541 aagtagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    15601 gtggctgttt ccagcctttt ttaggtgctt tagctgcagc gggaatggtc aaaggattaa
    15661 atgctttatt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    15721 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    15781 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    15841 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    15901 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    15961 ccagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    16021 ccaagaaatg gattcctgca gtcgtccaga cctttttggt tcctttttct gtgttattaa
    16081 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    16141 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    16201 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    16261 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    16321 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    16381 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    16441 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gccatttcag
    16501 gaatctattt gatggcatct ggaacaatca gctacacaat gggcggatta gggatttttg
    16561 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    16621 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    16681 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    16741 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    16801 gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca
    16861 tgaccctgtt cccgacgaaa catgcaattg gaatggtttc tgaaacaggg ctggaattgc
    16921 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    16981 agcaaggaga aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    17041 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    17101 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    17161 cctgaacggt taggtgaaga atggaatagg cttaactatt ctttttattc cacttcttta
    17221 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    17281 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    17341 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    17401 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    17461 tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta
    17521 cgaagaatca ctttattttc acccgtataa gaaatcagga tcccgcacat atcgctcgtg
    17581 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    17641 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    17701 atcacgattt tttttgcctg gagcagtaaa tacttggctt tttgcagttg gtcatgatga
    17761 atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt atttgcgatc
    17821 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    17881 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    17941 attttttttg ctacacggat gagtgtagac gggtggacat agcatcgctc agcgatctct
    18001 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    18061 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gttaccttct
    18121 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    18181 atataactta aaatagactt tttttgacaa gatatttaca aaaatccact agaaaacagt
    18241 aacttttgta aatgttactg gaaagaaacg gatttctatt gtttgttttg tttacaaaaa
    18301 agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc
    18361 tacgattggt ggaggcagca gttacacccc tgagttgatg gaaggattta tcaaaagata
    18421 tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct
    18481 tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat
    18541 ctatgcaact ctggatcggg aagaagcatt gaatgatgcg gattttgtta cgactcagtt
    18601 tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat
    18661 gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt
    18721 ggtcttggca atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt
    18781 cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt
    18841 gattggatta tgcaatgtgc cagtaatggc gatgatgaca gaaccagcaa tgcttggcaa
    18901 gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt
    18961 agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga
    19021 caatggattg ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat
    19081 gaatatgatc ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca
    19141 tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc
    19201 agagctattt gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa
    19261 acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa
    19321 taaagaaaca cagatcgtgg tatctacaaa gaatgatgga gctgttcctg acctgccggc
    19381 agattgtgta gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt
    19441 tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct
    19501 gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa
    19561 tccgcttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca
    19621 ccagcagtat ttaccgcaat tcaaggaaac aatcagccga ttaaaagaag aaggaatcga
    19681 agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata
    19741 atgggattca gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc
    19801 ggttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac
    19861 cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat
    19921 ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca
    19981 gaaatggggt tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt tccaatgggg
    20041 gatgaaaaag agccgaatga agctggcttg aaattttatg aggatgtctt taaagaatgc
    20101 caaaagcacg gaatcgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg
    20161 atcaaagaat acggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt
    20221 cgagtcatct tcaatcgata taaaggactt gtcaaatact ggctgacatt caatgagatc
    20281 aatatgatcc ttcatgcacc attcatggga gcaggactgt actttgaaga aggagaaaac
    20341 gaagaacaag tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg
    20401 aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca
    20461 tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa
    20521 aattatttct tcattgatgt tcagtcacga ggagaatacc cagcttatgc attgaaagaa
    20581 atggaacgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa
    20641 catactgttg attttatctc tttttcttat tattcttccc gagtttcgac taccgacaac
    20701 gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa
    20761 gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt
    20821 tatgatcgtt atcaaaaacc attatttatt gtagaaaacg gcttaggtgc agtcgatacg
    20881 ccagatgaga atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc
    20941 caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg
    21001 ggatgtattg atttagtatc agctggtacc ggtgagatga agaaaagata cggattcatt
    21061 tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc
    21121 aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga ttgatcaatg
    21181 atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa
    21241 acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa
    21301 gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg
    21361 actgctgctt tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc
    21421 cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc
    21481 atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat
    21541 gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa
    21601 gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta
    21661 ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat
    21721 ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa
    21781 gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa
    21841 ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg
    21901 tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca
    21961 cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt
    22021 ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg
    22081 attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt
    22141 cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa
    22201 caagtgtata aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtag
    22261 aagaacgggt tcaattgttc ttttactctt ttttttcgcg tgaaatttac tgaatctgat
    22321 ttatgcgaac acgtccaaca ccttttgata aaagaatcct ttcttgattt aagcgttttt
    22381 tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca
    22441 gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa
    22501 aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt
    22561 gaagatgctt tttatgcacc gttggaattc ggtactgcag gcatgcgggg aatcttagga
    22621 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt
    22681 ttcatggata cacaaggtga agaagcaaaa cgccgaggtg tcgctattgc ttatgactca
    22741 cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactggcaca tcacaatatc
    22801 ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat
    22861 ttgaagacgt ttgcgggaat catggtaact gcatctcaca acccagcggc ttacaatgga
    22921 tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg
    22981 aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct
    23041 aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa
    23101 atcaaagcag tgacgattga tcgtgaattg gtagaaacaa tggggaaaga tttgaaactt
    23161 gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct
    23221 ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg
    23281 gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa
    23341 aaagaaggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg
    23401 gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg ggcattgttt
    23461 gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta
    23521 ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctacc
    23581 atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca attcgaagaa
    23641 gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct
    23701 tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac
    23761 tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc
    23821 tattttgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
    23881 tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt
    23941 attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa
    24001 ctaacaacac cgccatccga cgtattgaaa tatacattgg aagaaaatgg ctggattgct
    24061 atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct
    24121 acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa
    24181 gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca
    24241 agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat
    24301 tattcaaaat gttgagtgat cccactcgtc ttaaaattct gctatatctg aaagacgggg
    24361 aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc
    24421 aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt
    24481 tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg
    24541 aacatcaata acgataggtg caaatagata aacactggag tgtgacaacg aaatttcttc
    24601 gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt
    24661 tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc
    24721 cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt tttcataaag
    24781 atcattcgca aaatcgagtt cagctctaat tttttcgata tcggaataag cagtatcagg
    24841 gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt
    24901 cgttagtccc acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg
    24961 tgggatcaaa ggtaaattgg caacttttaa atttttgtta gctaaaaaca agctcaaagg
    25021 cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg
    25081 atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt
    25141 ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccgg ttaaacgttc
    25201 aatttctgat atcggtggcg tcatcacatc aaaactaaat aattgatgtt cttcacaaaa
    25261 agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc
    25321 ttctaatgca tcttgcaatg cctgctttaa tgttttttca tctgtcacga acgttctgcg
    25381 gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc
    25441 agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag aatcggaaga
    25501 catggataaa acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt
    25561 acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca
    25621 atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt
    25681 aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa
    25741 tcaggaattc aaaggggtaa gttatgatac gatttatcgg aatctccatg attttgcaga
    25801 gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg
    25861 tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga
    25921 aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca cgatcgaagg
    25981 gcatagattc gagattttcg gaagatgtga aaattgtcaa taagggttta aataaaataa
    26041 catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata
    26101 atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt
    26161 taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt
    26221 gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct
    26281 cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct
    26341 agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa
    26401 gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa tctttacaag
    26461 tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga
    26521 cagaagaaga agaactaacg gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc
    26581 atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa
    26641 tagtcgaaaa atatatgccg gaacaacttt ccgatgaaga aattcgacaa cttgttcaag
    26701 aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa
    26761 ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac
    26821 tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca gttttttgtg
    26881 ttcgtaaaaa aagaactaaa aa
//
LOCUS       DACEKQ010000033        26869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEKQ010000033 DACEKQ010000000
VERSION     DACEKQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.035"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            325..888
                     /locus_tag="IW671_001257"
     CDS             325..888
                     /locus_tag="IW671_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898251.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            875..1759
                     /locus_tag="IW671_001258"
     CDS             875..1759
                     /locus_tag="IW671_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ0898252.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            2153..2650
                     /gene="dfrG"
                     /locus_tag="IW671_001259"
     CDS             2153..2650
                     /gene="dfrG"
                     /locus_tag="IW671_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0898253.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2722..4674)
                     /locus_tag="IW671_001260"
     CDS             complement(2722..4674)
                     /locus_tag="IW671_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0898254.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4681..4977)
                     /locus_tag="IW671_001261"
     CDS             complement(4681..4977)
                     /locus_tag="IW671_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898255.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5543..5797
                     /locus_tag="IW671_001262"
     CDS             5543..5797
                     /locus_tag="IW671_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898256.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            6194..7795
                     /locus_tag="IW671_001263"
     CDS             6194..7795
                     /locus_tag="IW671_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0898257.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            8029..8328
                     /locus_tag="IW671_001264"
     CDS             8029..8328
                     /locus_tag="IW671_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0898258.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            8462..8839
                     /locus_tag="IW671_001265"
     CDS             8462..8839
                     /locus_tag="IW671_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0898259.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            8888..11251
                     /locus_tag="IW671_001266"
     CDS             8888..11251
                     /locus_tag="IW671_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0898260.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            11569..12168
                     /locus_tag="IW671_001267"
     CDS             11569..12168
                     /locus_tag="IW671_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898261.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            12280..12504
                     /locus_tag="IW671_001268"
     CDS             12280..12504
                     /locus_tag="IW671_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898262.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            12856..13092
                     /locus_tag="IW671_001269"
     CDS             12856..13092
                     /locus_tag="IW671_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898263.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            13172..14026
                     /locus_tag="IW671_001270"
     CDS             13172..14026
                     /locus_tag="IW671_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0898264.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            14039..14476
                     /locus_tag="IW671_001271"
     CDS             14039..14476
                     /locus_tag="IW671_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898265.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            14796..15239
                     /locus_tag="IW671_001272"
     CDS             14796..15239
                     /locus_tag="IW671_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0898266.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            15384..15707
                     /locus_tag="IW671_001273"
     CDS             15384..15707
                     /locus_tag="IW671_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898267.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            16125..18935
                     /locus_tag="IW671_001274"
                     /pseudo
     CDS             16125..18935
                     /locus_tag="IW671_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19031..19786)
                     /locus_tag="IW671_001275"
     CDS             complement(19031..19786)
                     /locus_tag="IW671_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0898268.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(19801..21027)
                     /locus_tag="IW671_001276"
     CDS             complement(19801..21027)
                     /locus_tag="IW671_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0898269.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            21135..21962
                     /locus_tag="IW671_001277"
                     /pseudo
     CDS             21135..21962
                     /locus_tag="IW671_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22022..22384
                     /locus_tag="IW671_001278"
     CDS             22022..22384
                     /locus_tag="IW671_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898270.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            22618..23040
                     /locus_tag="IW671_001279"
     CDS             22618..23040
                     /locus_tag="IW671_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898271.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            23052..24467
                     /gene="cpaF"
                     /locus_tag="IW671_001280"
     CDS             23052..24467
                     /gene="cpaF"
                     /locus_tag="IW671_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0898272.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            24484..25299
                     /locus_tag="IW671_001281"
     CDS             24484..25299
                     /locus_tag="IW671_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898273.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            25299..26096
                     /locus_tag="IW671_001282"
     CDS             25299..26096
                     /locus_tag="IW671_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898274.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            26114..26476
                     /locus_tag="IW671_001283"
     CDS             26114..26476
                     /locus_tag="IW671_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ0898275.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            26648..26869
                     /locus_tag="IW671_001284"
     CDS             26648..26869
                     /locus_tag="IW671_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898276.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT         9885 a         3506 c         4858 g         8620 t
ORIGIN      
        1 aattataatc acaaacttaa tagtaattac tattgggatt acaacattaa caaataaagc
       61 tttaatatca agtatgattt taatagtgtt tgattttatt atcatttata gtttgctgtt
      121 tttaataaag aaagtaactg aatatttaaa tccgttaggc acgaaataaa ttttaattga
      181 ggttgaatta tagcggtaaa tcatacaaaa tagaaatgta gtcttgttaa gcgtcttgac
      241 tatctaattt tgaatgagga gggttgtata tagaaaaagg gtagttaaat tcgtagatca
      301 aaaggctacg aaggagaata aagaatgaca gctatttata aagcactaaa aaattctaac
      361 gtatcagagt acgttaagtc gagagtatta aataataatt tagattacat tttagaaaat
      421 ggtgttgctg atttaatcag taaattaagt gatcctatag atatagatca tttggtggta
      481 tggcaagatg gtactttaga agtatcaata tatggagaat tttatagtag aaataaagta
      541 actacattca aaaatagtat tagtggtcga gaagatttaa aaggaatcaa taaaaatagt
      601 aaattctata gaacaaataa agaattgcta gaaaaaattt caaagcgtgt ttttgaattg
      661 gtgaatccat tttcaaaaaa acgtagtaaa gaactttttg gaatttttta tgatggtttt
      721 agtgcaaaaa gtaatgtaga aaaatttttt tattttaatg attcagctta tattgcggtt
      781 aaagacagta aaagaatccc tgaagcaact ttagaatatt tagagccaat taaattgtct
      841 gttttttatt cactttatga gaaagaaatc gctgatgaag cggtttaagg ggagtaaaat
      901 gagtaaaaaa ataggcaaag agtcattatc gctcggattg aaaaaagaaa ttgttaaatt
      961 taaaagtatg tatagagagc cggaatttag actcattttt caattagata tagtgagagg
     1021 gctgaaaatt agttttagtt gtttaggtta tctggtctgg ctattcattg ctagagtagg
     1081 atggagcaat gcttatagct tttataaact gttcgccgga actgatcgat taaaagaatc
     1141 attagaggtg aaagaagcct taaatatttt aggaagtctt gctttcagcc tgtttatttt
     1201 tgctttattt atcttacaag taggaaaaat aatgatgtca acaaatgaca attatattaa
     1261 ttatttgaag ccttttcaaa aaggaataaa aatcatttgt agacagggca cagttttatt
     1321 tctacttagc ctgtatgagc atgaaattca ttttcaaaat tattataata cgacaactaa
     1381 acttggtgca tttttactcc tatatttagt tagtaatata gctgaaaaaa tgctgtttaa
     1441 acatattgta aagcaagtct tttctgatat tcaaaaggaa agctattgtt taaaaacacc
     1501 ttatggaaat ttaaaaaatg ttgagctgtt tttatttaaa atggatagta agtgggataa
     1561 atatatctat agaagtaaag aagtattttt aactggtagt tataacaaaa ctcttcagga
     1621 gagcctgtac gacacaggag caatatttaa taatgaacct atggttttga aagatttctt
     1681 tggagattat gaactagttt ggaatccaaa acagcttagt gaacaggggg cagaaaatga
     1741 atttgctgaa gttcactaac atagtgtaca gaaaaacatt atctgaaaga agaacgcctt
     1801 caaaagaaag tacaagaacg attgggccgt acagaagact aacgaaagat cgtaaaaaaa
     1861 aggaaagaca atgaaaatta agtagttcag ttttggagta caaaatgaca aaatgtactt
     1921 caaaatggac tacttttttc attttaactt acataccaaa ttacatacaa aatgatttat
     1981 aaaatgcaat tgaaaatgat aattgaatga aaatttgtga ttttgtggta ttatgataat
     2041 ataacttata gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt
     2101 tgatagaaat aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt
     2161 ttctttgatt gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg
     2221 gaggattccc aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt
     2281 aggtaggaag aaccttgaat caatcggaag agccttacct gacagaagaa atattattct
     2341 gacgagagat aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt
     2401 ttttgagtta tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa
     2461 tttgtttttc ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg
     2521 agatactttt tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg
     2581 gataaagaat gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt
     2641 attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg
     2701 gattactcca agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt
     2761 atctgtaatc cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg
     2821 gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt
     2881 gaagttagcg taaatatagc tcttcttatc accacgagct gttgttaatg gtctttcaag
     2941 agatgatata taacgtctta tctgttgaat aaagctattg gtcgaatggt cattaacatt
     3001 taatagcatc tttgcgattt cgtttggttc cagagcagat aaatccgttc tacaatcaac
     3061 tgaataaaat cctttatcct tagtggctaa tggatgtttg atagggttat cggcatattt
     3121 gcgatatctt tctccatctt tactaatagc ctcttcgtgg aattgatgac ttgttagtaa
     3181 ttcagttaga taatgagatg ccaaagtccg tagaaacttt gttttgaatc ctctgatatc
     3241 tccccaatcg agcagttctg cttttgcttg tccgaattct tttaagcatt gttttctgct
     3301 tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg ctcaacctta attctttcgt
     3361 gaatactcta taaaaggcgt ttctaataga gaagtcatca tcactaatca ttcgccattc
     3421 gtcggcatta accatttgtt taattaacca atagtgagca gttgtagtat aaggagcatc
     3481 aacatttaaa ccatcgacaa atctactcct attagtaatt ttccctaatt cgtgaagata
     3541 agcattccta ttttcgctat cgttagcgga tggttcttgt ggataataag accaatctaa
     3601 cctatcattt ttacgactaa aagtattgag atggtcttct ttgaattttc ttgtatcttc
     3661 atttaattca tccattgaaa tattccaatc ataggcaaca tcagaacgga acacatatct
     3721 tgataagacc tcagcagata caactatata cgtttgcata ttaaggtctt caaaccccgt
     3781 atatttcttt gaaccttgtc ctttcttacg gacgttattt aggtagtagt gcatcttgtc
     3841 cgtattaagc cacatctcgt taaatttctt tgtttgaagg ggttgtgtct catatcgttc
     3901 taaaaactcc aaacaacgcc tatacagcca ctccagctta tgatagtagg ttccaactcc
     3961 aattcctaaa atatcacaag tacgactgac tgaaaccctt ccaacaacca tcttagcgaa
     4021 cattggaaga atggtgttct tttgttggtg ataagtagtg gtttcttctc tttttggtag
     4081 aacattagtg aacttcttac acgccttaca ttgatagcgt tgagatttgc ccctactttt
     4141 cccccttttg taaaattgct tcggctcatt gaatggggtt gattcttctt ccgagcagcc
     4201 ttctttatga aattgataat ctggctcaac atcttggatg ctattgattt ctataaggcg
     4261 tttaatttct tcaacaacag accaattaga taaaggggtt actgtacagt tttgcgatac
     4321 acctctgcca ataggattgt cattgcagta cataccttta tgacctttat ctttggaact
     4381 accagtcatt ttatatcgac ttggtttccc ttttacatct ttgaatttgt gctgttcttt
     4441 cccaaaatta caacaaaacg gattgatgca gtaattatat tgaatgggat attcagaacc
     4501 gttccattta aaagacgttg gcaagaacaa cattttgtta taccttgttg caaatcgttt
     4561 tggagttaat aaatcatatt gtttagccct atcttttaat tcttcgtttg aaatcggaat
     4621 tttaacatct acaatttcat cttccttcgt ggctaatctt ttaagcttgg tcattgtcta
     4681 tcaacccact ctatctttga tacccatttt ttcaacaatc cgcttattac ttcgcttgtt
     4741 ggcaatccat tcaataaact tcttatcgtc taaaatgttc aataagaatt catccactgc
     4801 ttcgctttca gtacgctctg catattcagc gtattgctta actatcaccc ttacttgctc
     4861 tgatatttcc caatccacag acttagcgtt cttatttttt ggctcaagaa attgcatagg
     4921 aaaatgcacc cctttttgct tagtaaattg taaccatttt aacacaccaa tttgcattcc
     4981 tacaataatt atttcacgtt cgcattttta tatacatttt aacttacatt ttgtggtgta
     5041 aattaacaac aaacgttgat ttaacaataa aaaacgagta tcaaattcag ttgcaatttg
     5101 atactcattt aggtttacat tttcactttt tgtactccaa aactgaacta cttaaatgaa
     5161 aatgtctttc cttttttttt gactacaagt agcctgaaaa tgaaatttca aaagactaca
     5221 agtagtcttt tgtggcaaaa ataaagagcc aacgaaagct accaacaatc gttgactcgt
     5281 aagactgttc gtagtaagaa aaatctttgg caatccacga agtctttatt tcataattta
     5341 tataaatgat tatacagtca ctttcctgaa tttgcaagta aaaaagctaa gaaatattgt
     5401 ttaagcgttt ccttaaaaga atctttaaat aaattttttt ccaattgaaa acatgactac
     5461 aagtagacta tttcttttat tccaagttga ctacaagtag tcaaggtgat ataatatata
     5521 tatcaagaaa ggagaaacaa aaatgtataa cgtaggagat caaatagatg agatcgtaaa
     5581 aataggtaat gttaaacttg ttaaaagtaa gtactatatt tcagaaactg aaataatttt
     5641 taatactgaa tcaggatgta ttctaatttg gaaactgcct tttcccaaag acgaaacaac
     5701 actgaaaaaa ataaatactt taaaaatcag atattgcatt catgaaatta ttgataaaaa
     5761 tacttatgtt ataaatcaag taaaagtgat ggggtaaaaa tcttttaaaa aaactcttgt
     5821 taaaagtaat tctattagct tataatgttg gtattagttc tgaagccaaa ggaacactaa
     5881 aaaattctag tagaaaagtt agttacagac tctctactat gtatgaatgt aacgaaaaaa
     5941 atagcgaaaa agataattaa ataagactac caatcttatt taatggaact tctgtagtaa
     6001 gaaaagcgat ttggcaatcc acgaagtcca ccaagatgag ctaccaactc attttaattt
     6061 ttgcacgttt aaagtttagc atttaagtgc ttactctaaa gacatagtgt aattttagga
     6121 ctccttatta cggaatctaa agttacccta tgtctttttt gctattcaaa attgatagca
     6181 aggagaaagt gcaatgttga atagttgcga gtatgcggac gttttagcta aaattcttaa
     6241 aaatggaatt aaacagttta atagcaaaaa tctagttgct tttaaaaaga atagcaataa
     6301 gattggtgct attgccggat ttacttctaa aacaaacatg ataaagacta aaggtcttat
     6361 atatcgttct attgatcgtg tcttggaaga ttcagatgta ttaacacact ggactcctaa
     6421 cgtgtattca tggcttggtg gcggtgaaaa caaaacaatt tttggtcaca aagaagctaa
     6481 cttaattcag attaatactt ttgtagcaga tattgactat ccagtaggag aaaagaaaac
     6541 ggatttgaat ttactagttc tttatctatt ggaagaaggg ttattgccta cactgattgt
     6601 agatacgcca aaaggttatc attgctattt tctgattcaa aactataacc aagaaactga
     6661 ttcatttgat aaggcaagct atatttctaa ttcaaatgat tttaaatctt tgagagttgc
     6721 taaaagaata tcagaaaata tcagaaaatc tatccaaaat cgtttaccac aagtcgatat
     6781 gggctgtaat cattttggta tttttagatt tccaactaaa aaaaatattg tacattatga
     6841 acctaatttc gtagatacgt ttgaagggta tttgaaatgg tcaaaagact ttgaagaaaa
     6901 agaatatgct ttaaagaggt ctaacttgtc attattagct cctaattcta ctggcaagaa
     6961 agggtctaaa cgacaaattg atactaaatg gtacgacttt ttaattcata cggatattgc
     7021 tgaaggagat agaaataaaa ctatttttac cttagccctg gcatgtaaat caagtggctt
     7081 atctattggc gagtgtatgg atcaattgga tcaacttcaa ttcaataatg atttgtccta
     7141 ttctgaagtt gagagaacaa taagaagtgc ttatcgtggc gattacaaag gtgctaaaag
     7201 ttcatatgtc aatgaaatta tcgaaaatta tgctacacct accgagtttg aaaaatactc
     7261 aataaatgta aatagacaag ctgttaaacg taaagggaat cgtgtcgatc cgtctaattg
     7321 ggtgaaattt gctaaaccta gagaagaacg aaaatatagt cattttgaag aaagtaaagc
     7381 tgatttatta gcttacttag aaagaaaaca aagatcattg aaaaaaaatg agttgttttt
     7441 aagtgtctct atgggaacta tatctgaaga aacacaaatt cctgtatcta cgttaaaggt
     7501 gattttaaaa gagcttagaa cggcgaatac agtgatatta cgaacgtctc gtggtcggta
     7561 tggatcgact aaaatagcaa caagcaaatt tgtggaagct aaaatattaa atacagttat
     7621 tcagaataaa aaagataaac aagtatttgt cttagaatta ttaggaacag caaatagcca
     7681 cgtagaaacg attttaagac gttatgaaga caaaacagta ccgttccatg cgaacatact
     7741 gaaagaaaga gaaaagagca aacatgcgcc aattagagga gctaatacag gttagagttc
     7801 tatttatttt tttgattttt aaatagctaa attgccaatt aacatatcta gtgtttctgc
     7861 ccactcctat tcctgtttta gcgtatacta atgattagta gttagtactc tcttttatgt
     7921 atggagtgta tagaactatt tttacttgtg tcttttccct taatccacgt gtacggaata
     7981 cagcttataa ctgcgtatgc agttatgttt gaaggaggaa gattttcttt ggtaattaag
     8041 aaatatgttt actcacaccc gatcaatgta tttattatcg gtaagctagg tttatctgtt
     8101 gaggtgtttt gtgagttata tggatttaaa caaggaactc tatcgtcttg gattactaga
     8161 gagcgtgagg tagctgattt gcctatttct tttttgtatg ctttgtctct agcttcagga
     8221 gggaatatga atgaggttta tgattgcttg tgtgagtatg aaaaagagta taaagagtat
     8281 ttgaaaattt cagccccaaa acggaataaa aaatatattg aagattagag aaagaagaaa
     8341 agaataattt gttttttgac tacaagtaga cagatagcta ctctgttttt tgatagtttg
     8401 atattggctg tttttttgtt agaattaata tatatcaggt aaataaaaaa gggggctatt
     8461 aatgaatatt gatgggattt ataatgaatc agattttaaa aaaggcatag tagatattga
     8521 tagaaaagag tatttatttc ctgatgaaat aggtttattt aaaattgtag gtgaaactgt
     8581 ctgtaggtat tgggggaaga aaggaaatat aattttactt gttgatacga ttgagggaag
     8641 aaagttaaaa ttttcgtttt ggagtaatac aaaatattgt tgtgaacctg tacctaaaag
     8701 ggaagtagat gtggctaaag aaagtgtacg atttttacct gatcgatcaa aaataacttt
     8761 aatttgtgaa cggacaagta atgctaaatt tacaagaatt agagatatac tactttcgac
     8821 tacaagtagt cagtcataga gtaagaactt ttggttaaaa atcaattgaa gaaagtggag
     8881 gaagtatatg gcagtagtta tattagcaga aaaaccagat caaggagcaa aatttgcttc
     8941 agcattggcc ggaaaaaaat tatcgaaaaa gagtgggaag tatgaattta aaagtgaaat
     9001 atttggagat actattgtta cttggggaat aggtcatttg gtaggattat cattacctga
     9061 aaagtatgag tggctaccga ataaagataa atggcattta gataatttgc cttttctacc
     9121 taaattcaat gaattaaagt atgaagtctc taaaggaaaa aaccaacaat tttcaatagt
     9181 gaagtcatgt ttagaaaatg cagatgagat aataattgct actgatccgg atagagaagg
     9241 agaaaatatt gcttataata ttttcaaatt atgcaagcag tcgattttta ataaaccgat
     9301 aaaaagatta tggattaatt ctttggttca aaaagaaatt atcaaaggat ttaaaaatct
     9361 gcgaaattct gaagaaacga tctcttactt caaagaagct aatgctagac aaattgctga
     9421 ctatctagta gggatgaatt atacagagtt gtattcatta aagttgcaag aaaaaggtat
     9481 atcgggtgtg gtatcactcg gtcgagttca gacaccggta aacacacttg tggttgaaaa
     9541 cgatttggca attagaaatt ttagaccgga aaaatataaa acgattgaat gtcatacaac
     9601 cgataagcaa ccaaaagcaa tatttaagaa tagtcttgaa tactttaaat tagaaaaatt
     9661 tgaagaagac actaaaaaat tcggccttga ttccgccagt actggaatca taaaaaaaat
     9721 tgaggttagt aaaaagcaaa cagaaccaca aaaattgttt agcttgggtg gtattcaaga
     9781 atacgctaat aaaaaatgga aatattctgc taaaaaaaca gataaaatta tccaaagtct
     9841 atatgatcgt ggatatttat cttatccaag aaccgatagt gaattgataa ctacaaatga
     9901 atttgagtat ttaaaagcga atttagacgt gtataagagt gttttaggga tcagtattga
     9961 tacaccttgt ttagagccta aaaagcggtt tgtagataat gacaaagtat tagagcatta
    10021 tgcactaata ccaacaatgg atattcctga tatagcaaat ctgacattag atgaaaaaaa
    10081 tatttatgag atcgttacta ggcgtgcgtg tctaatgttt gcagaacctt acaagtacga
    10141 aaatacaaaa gttacattag atgtaaacgg cttagaattt accacaagtg gtaatgtacc
    10201 tatatcttta ggttggaagc aagtagatac taatttagat gaatctgatg ataaagagga
    10261 aacattacct aaatttcatg aacaagagac aataaatata gatattagat ttattgataa
    10321 attaactaag gaacctaaaa ggttgactga aggtaaatta ttaggtaaaa ccggtattat
    10381 ggcgaaatta gggctaggaa cgcccgctac acgttctaat attgtagaca ctcttctaaa
    10441 tagggaatat attaagatag aaaacacgaa tgtttatcct actccaaagg gatatttatt
    10501 atgggatttg acaaagaata aagacctatt gattggaaaa ccagaaaata cagcgatatg
    10561 ggaagaatca cttgggaaaa ttgcgcaagg tagctataca caggaagctt ttttaagaaa
    10621 tatctataac tatatcaata agactgtttc agatttgaaa aaagtagaat ttacttcgga
    10681 ttatttagaa aagactgtaa atcaaaataa atttgatata ggtagttatc aagtggaaga
    10741 aaagaaaaaa gtttattcta ttttaaatat tactagtggt gaacagttcg tgatatttaa
    10801 aaatatatca ggaaaagcaa tcaataaaaa agttcttcaa gaactattac taaataatcg
    10861 tacagataaa gtcctttccg gctttaagtc aaaagaagga aaagctttta atgcaagatt
    10921 acttttcgat ccaaatgtga tgaaagtggt aatggagttt gaaaagaaca gtccaaaagg
    10981 cgataaaaag agcaaacacg atacagtaaa tggttatgaa atagttgaaa aagcaaaagt
    11041 atttgaaata aaagaattag caacaggtga aagttttatt ttctataaaa ataatagtgg
    11101 gaaaacttta agttcaaaaa cagtcaaaga attgttagaa aatgggaaaa ctaaagagaa
    11161 agtaacgggt tttataagta aagacaataa aaaatatagt gcttatcttg tctttgataa
    11221 taaaaacaaa gttataaaga aagaatttta attcttagtg gcggaaatca tttgtttttc
    11281 cgcttatttt tattcaaaag tgcccaaaat ataggtatac tgaattaagg ataaatacaa
    11341 aactagttag aggacagttg aaaaaataca aacaaattag attgctattt tgttaaattg
    11401 gaagtagaat aataccgttt tgatatgtat agtgtgtttt accaataaaa ttacataaac
    11461 gtgaatatat ttttatgaat taaatgtaaa atgagagcaa ttagttttaa aaaagattat
    11521 aggctgttaa tcttagcata tataatccat tggaaagggg gagatccaat gaatctaaag
    11581 aatacaaact caaacaaatc tacactatat gtaatttcag atgattatat aaataagctg
    11641 aaaaaggtgg actatcgtgt ccaaaacaac tatcgagaac aacgattata ttttaaatca
    11701 aacttgaaaa tttctaacaa aaatgaagaa ataaattatt atgtaccatt ggcttcagca
    11761 aaagaaaatc aacgttcttt gactaataaa tcagtgttta agatttatgg tgatcgagaa
    11821 gaaaaggact ttttaggagt acttcatata aataatatga ttcctgtgcc aaaagattgt
    11881 gcaaaagtat ggacacctaa tagcccaaat gttgatgaga agtataagat gttagtaatc
    11941 aaacaggcaa aatatttgaa attacatgag tctgaaataa atgaatcatg cgaattactt
    12001 tataattcta agttaggtaa acttgatcct agttacttta agaaaaatcg taaagaagta
    12061 attacttatc aaaaaataat gacgaatgta gaatcattgg aagctgttag tatagcagaa
    12121 tcccaaagaa atattcatgt acaaactcaa agtatagatg tagagtaaga taggctaaac
    12181 ctatcttatt ttattattct attggatatt atgatacatt gaattttaat cgaatatgta
    12241 tttaaatgtt attatactgt agaagataaa acttaaagga tgatttctat ggcgagaccg
    12301 agaaaataca atacagaaga agaaagaaaa gaagcaatga aaaaggctag gaaaaaatgg
    12361 gttgaaaaaa atcaggaaac tattgcctat ataagagcca aatcatttgc taaaaaattc
    12421 attgaaatgt ctgaagatga agatattgaa ttgttggaaa aatggttaga agatcggaaa
    12481 aataacggtc aaaatgatga ataataacca ttgactacaa gtagtcaatg gtgtagaatg
    12541 gttttaggga tagaaatatc ccttttctaa aagtctaatg actacaagta gtcgatagag
    12601 ggatcgggaa taaaaacaga cgattatggc ctaaagaaag gaggaatatg aatattttga
    12661 ggaacgattt attaaatgag cgttgataaa tttcactaaa tattttctta atatctatgt
    12721 attgttcaag cgtagaaaaa aatattgaaa ttattacaaa accgctcata atggaaaata
    12781 acgaattata ttaatgaatc gttcactttt gacctttttt actagatttt tttgtaggac
    12841 ttttagagaa tttttttgtt atctgattat aaggaggagc taaagttgat agagaataga
    12901 gtactggggt tcgtagagtg taaagataat ttagacgata ctttagaagt ccttgaaagt
    12961 attatattta acaccaaagt tctggatttg aaagcaatta atggtgatat gacaacacat
    13021 atcatattag atgaaattag cgcaaaagag attggcgaaa ctttaatagc atgggctaat
    13081 aaagagctgt aaaagataat tttaaaataa agtggccaat tgactacaag tagtctgtat
    13141 agtataataa attatcaaaa gtgaggtata catgatgaaa tcaaattttt tgggatatgt
    13201 aattgcaatc aacatgttta aagggggagt tggtaagtca actcttactc agctaatggc
    13261 tgatattcta tcggctagag gctttaaagt tttagttatt gatgttgatc cacaaggaac
    13321 agtaactaaa aagtttcaaa gacaatatga tttaaaagtt gttgggaaaa tgacattgtt
    13381 tgaagcagta attaatatgt ctttcttaga ctgcatttct cctgttaaag aaaacttaga
    13441 tattgtgctt ggttcagaac aaatgtcaag gtttaccaaa gtattaagag atatgtggag
    13501 aagtactggt tttactgatg aaggtgaaga agtcttttgg aatttgttta attatatgat
    13561 cggtaatgat aatttaagag atcaatatga ttttattttg tttgacacta ttcctacagt
    13621 ttcagaattt acagataacg tttacgtggc gagtgatttt ttagtaattc ccacgcaaac
    13681 tgagcaagat tctatagaca acttgcatag tacggttact aattatcaaa ctgctgtaaa
    13741 agaagtaaac ggagatttag aaattttagg tattgttcca tatctagtgg atactaaatt
    13801 aaaaacagga ttagaacttt tatcagaact tcaagaagaa tatggagact tagtttattc
    13861 tcatattgtt tcagaacgag gcgtaattaa acgttggggt agagaaggac taaataaatc
    13921 tatcccacat tctaaaactg cttatagcat gtatgtagac attgtagaag aaacgttagt
    13981 acgtttagaa aaagctacag gagaaaatat tttagaacgt gtttaggaaa ggtgttgcat
    14041 gatgtcgaaa actaaaaaag taactagtgc cggcagtaat ggttcgggta atccgttcgg
    14101 aataaaaagc ggtagtggtg gatttaatat aggacaacaa atgttaaaag gtaaagctgg
    14161 cattaagtct actggtgata aacggaaatc tgttagggta ccagttgaat tatacgatga
    14221 agttgttaaa gatttatcat acggggctaa atattcatta atgacagaaa ttttagcttt
    14281 atttttaaaa gaatttgaaa cacatggaaa tagtatagtt actgattttg gcaagttgaa
    14341 aacgagacca gtaaaaattt ctgaagaagc ctttaaagtt ttgaaagtta taaaatttga
    14401 aacagatcaa tctaatacta atattattgc ttcagctttg acatggtatc ttgataaaag
    14461 aaaaggaaaa gcataatgaa atgaggtaag actatggatc ataaatcaaa ttattcatac
    14521 aaagaaaatt atatttggaa gtatgtagta gccgtttttc tactggtagg tggaattatt
    14581 gcattggtta aatgggctat aattcctaat gctttagtat ttatcaattg gtttgtccct
    14641 aatttttgga gtttttttgg ctatatattg ataatctggt ttatgtttaa agtactctct
    14701 tttttctggt cgcctgtata ttctttgctt ggaaagtggt cgtacctaat tttaatagcg
    14761 acagctattt atatcattat gcagattaac taattatgca gattttagaa gatattaaaa
    14821 tttattggaa aaggataata ggctatacat tagttcttct tgtagtaaat ttattccttt
    14881 cgatgatagc gttggtcttt gttaatcaaa gaaaagacaa tgtaactgaa ttagtaacgt
    14941 ttatttcgtt tttcataagt gggatttttg ctcctttctt tgaagaaacc ttaatgagag
    15001 gaattatcca aaaccaattg gaattaaaaa caaatttgtc tacaagaagc atatatataa
    15061 ttgttgcaat tttgtttagt ctatcccact taaatttata ttttattcct tattttatga
    15121 cgagtttaat tttgtctttt gtctatgata attcagataa aaggctgatt gttccggtta
    15181 ctattcattg tttttacaat ttaattgtaa tactgttaag tgtagttttt tatacttgaa
    15241 aaaaatagac gaaaacctaa aaatacaagg tttttatcta ttttttttat tttgttttta
    15301 tctatcaggt tgatagaaat tgtcataaat gatataatta agtatgaact gatttagtaa
    15361 aaaaattaaa ggagtgatag ctgttggcaa gatcaagaat tgctaaaatt gaacagacta
    15421 aaaaggataa aaaaatttct aaaaataaaa caaaattctt taaagctgaa gatgttaaga
    15481 ggatagcatg gtctgtgaaa acatttgatg aagatcgtta tgttaaagtt tcaatgaatg
    15541 gtctatttgt acagcttggt aacttcagta attttaaaac gattgatgaa ctttatccgg
    15601 aaataagaaa tattgtggat agtaaaggtt taatcgtggc taaacggaaa tcaagaaact
    15661 ctggtcttgc ttactgtaaa aataaaggga tcatgattcc ggcttaaatc agtgaaattg
    15721 ttctttgaaa acttaatact actcgtactg tagcaatgtt gctatgttac agataaaagc
    15781 gtaaggctaa taagcaaaat agcaaccaag catactgtta tttcctttgc caagaggtat
    15841 gtaaatacgt tagcaataga agtgataagg ttagtatttc acgccaaaaa atacaaaagt
    15901 cgatccattg aaactactaa ttatgaggtg gcgtaaatgg taattattca gaatttctat
    15961 ctaggaagaa cctatgaaaa ccgtcctaga gatcagaata ttgacactaa aaaattcagc
    16021 acagtaataa atgtgctaga tacagggtaa ctctttacta caagaaatgt agtaaagagt
    16081 tgttatgatt ctttattaca tttctttcta aaggaagtgt gataatgaaa aaactaaaac
    16141 tgattagcat tgctctattc agcctaatca ctatgtcaac ggctcgccaa tcaattgttt
    16201 cggctgaagg aacgtctacg aaagcagtag tagaagagtc tggcgataaa aaagttgcta
    16261 tagacgacaa agtgaaaaac gaagcggaaa aagcgactgt ggttcaaggg gcagacggct
    16321 tgatgtatgt ggtcgttgat gaaaatgatc ctgaaaaatt gctagaagct gaatctcctt
    16381 tgaaaaatca acaagaaaat accggcgctg ttgaagtcgg tgttgatgaa aagagacaag
    16441 aaaaggctga taaagctaac gaaaaagtga aaaactttga cgatattgat tacaacgata
    16501 ttagttcgtt gcagtcagcg ttgaatgatc cgaaattccc ttttttatcg gaagatgaat
    16561 taaatgaaaa attgcaaaag acaaagaata catttgagtc tgaagcgcta atgaaaaaga
    16621 caaggggata tttcagtcaa aggattttag agttaagcaa taatttaaat gacgtaccga
    16681 aaagagcgaa agtgagtcgg catgctttag ttagtaaccg tgcttcacaa gtaagtgaaa
    16741 cacgaataaa ccctccaaat ggttatccgt gggtgattta tttaattagg agcaatggtt
    16801 atagttatac agaaaattat gctattagaa gaactattga tgggaaagaa gctttttgtt
    16861 tacaggccgg tgtacaagcg acgaatacat acactggttc aacaagtggt tatctttcta
    16921 attcgcaaag agaaatgatt agttcgattt tatcagcata taaagaatta ggcggttggg
    16981 ctaataatga tgccggaaaa caaagatgga tagcaactca aatgcttgtt tgggaaaaca
    17041 gtaatgaaaa gcctaaaagt ataactgtta cgccaaattg gaaaaacgga gctactaagt
    17101 ggaaaaatga tttagtggct cgtgcaaatg ctattagagt aagaacaaaa tgggattcta
    17161 cgtctaagac ggttgatgag ggagataaag caacctttaa agtaactaat acttatggag
    17221 atcataattt gcatgtgaaa tctgtatctg gtggcggcaa agctaccata agcaatggta
    17281 ctttaactgt tgatactaca ggagccactg gaaacaaaat cactgtagat atgattaaag
    17341 gttctcaccc ttcagacgcg caccctacaa cggtatggag taatggttct tatcaaaagc
    17401 ttgttaccgg ttctttttac attagtactt ctgttacggt aaatgttaag agaaaaggaa
    17461 ctgttgaagt tttaaaagtt gattcagaaa caaaaaaacc attatcagga gcaacattta
    17521 gatttagcta ctcaggtaaa acaaaagatg ttactactga ttcgaatggt aaagcaaaat
    17581 taactgaaag gttgaaagct ggaactgtag taaaagttaa ggagataaaa gctcctaatg
    17641 gctaccaact agattcaagc gaattttcaa ttacagttaa agaaaatcaa aatgttacta
    17701 ccacaagaac caataagaaa tccacaggtt ctgtagaaat tgaaaagata ggcgatttag
    17761 atggattagg actaccgaat gtagtattca cgatttataa ttccgataac aaagttgtaa
    17821 aagataactt aaaaacagat agtaatggga aaataaaagt cgatgaactg caatttggta
    17881 aatattatgc agtagaaaaa caaggtgtta ctggttatga tcccgatggt aaaaaataca
    17941 attttgaaat aagtcaagat actcctttta caaaaccggc taaagtgaaa gtaacaaata
    18001 tttatgaagt atccataagc gaaaaagtat cggacatgaa tgaaagtcag gtattaaaaa
    18061 ataattggtc tgctgatcca aacgattatc ttcaatatga cattacaact ggaaatctaa
    18121 aaaaatcggg agaaagaaac gtccaaagtt tcattatcga agggttttac gacaataaga
    18181 atgtagatgt gtcaaaaagt gaagtatatg ttggatcaac aaatgtaaca aactcttttg
    18241 acattaataa cgatactaac aatggaaaag taattgctaa ggcaaagaca agcgtattaa
    18301 cgaataatga tttttataat aagtcctata atttacggat cactatgaaa attaaaaaat
    18361 ctactcttga aaacactgaa aagcaaaaaa atttaacgat tgttaaaaat agtaaagtta
    18421 ctgtcggttc aaaaatctct aaatctgcta gctctaatga tgtagaaacc gttttatatt
    18481 caagaaaagt tactatcaat catattgatg aaaaagacaa acaccttttg aaacaagata
    18541 ttgattataa atacgatggg gaaacgtatg aatataaacc tagaaccgat ttatttgata
    18601 aagatggaaa caattataaa tctgatgtaa tccataaagg gaaaattgat ggaaaagata
    18661 tagttttaaa cactccctat catattccgg tactttctgt gaatgtggat cggattcaaa
    18721 ttgatacggc tagagctgtt aaaaatggct atttacctac aaaaatagaa ctaagtaaaa
    18781 aagaagaata tgaaaaagaa ttagaaaaaa ttgtattcaa agttaaaatt acagatatag
    18841 ataacaataa agttgtttat gatgaaaact ttaaatacaa agaatttaat agtcatatgt
    18901 cgcttgattt acctacagaa tatttaacaa agaatgtcaa tggccattca aaaatgtagg
    18961 tttttggcca tttagaaatg tcgttttttg gccaccaaaa atgtaggttt ttggcccata
    19021 aataacaagg ttacttatac gacaactttt tttctttata atctttaatt cgataagact
    19081 ttccagtaat ttgaataatc ttagcgtggt gcactaatcg atcgattagt gcatttgtta
    19141 attttttatc ttgaaaagtt tcagcccatt gagacaaagg aatattcgtt gtaataatgg
    19201 ttgatttttt ttcatagcgt agattaatta attgaaagaa taactttgcc ccgtcagggg
    19261 taaaaggcaa ataaccaatt tcatcaataa ttagtaaatc gtaagaagca tattttttta
    19321 acaatcgatc taaatttcca cgttgatttg cacgatttag tcgattgact aattcattgc
    19381 ttaaagcaaa atggcaagaa taacctttgg ttatagcttc tagtgtaatg gcaatagcta
    19441 ggtgagtttt ccccactccg ctatttccaa taaacaatat attattattt gtatccataa
    19501 agcgtaaggt acacaaatct aaaatctctg ttcgattaat ctttggttga aatccaaaat
    19561 caaagtcatt gattgttttt ttaaacggta aattcgcctt tcttaaacga ttttcttttt
    19621 gtcgttcatt tcgtgagttt atttcttccg tcattaaatg atttaaagct tctacaaaac
    19681 taagattttc ttgaacaatg gtatcgagat attctggtaa aatttctctc gtatgtttta
    19741 aattcaaatc ttctaatttg tttagtaaat gataataatt agacataatg attcacctct
    19801 ctataattcg tcatacgctt gtaaattttc atcaacaaaa cgttctaatt cttcgtcttc
    19861 taaatgttta aaaacgtcag aacgtagaat ttctatatag tcatttcgat gataattaag
    19921 caattgatga gtcatcgtat gtgtgcgaat aagagtagag tcatcgtaaa tataaagttt
    19981 atcttctttt acacgaagtg taagaatttt accgatgtaa tttactggca cagaatattt
    20041 attttgattc cattgaacca tcgcttcgtt agatactttt cgtttaattt gtttttcaac
    20101 agaagcataa attttaagtt gattcgcatc aaaagaagat agttgttctt tctcgtatga
    20161 ccaacggtca ctaggacatt catgaattgc ttgagaaatt tcttcagtat ttaatgaatc
    20221 catgattgtt tgaataattt tttctaattc gtgagtagat tcaaattcat aattaaatac
    20281 acgaattcgt tcaattgttc gtgcaagagc ttcaactttt ccttttgttt gaggtcgaaa
    20341 agcacgacaa gcaattggaa gaaaacctgc ttctttcgcg tattgtcgga accgctcatt
    20401 aaaaataact ttagaaaatt gactacgact acggtcaaca acagttttca tattatcaaa
    20461 ccatatttct gtaggaacac ctccggtgta ttgaaaagca ttagttaagc aacgaaataa
    20521 agtagtttga gaacgatcaa aagttagttc taaatactta attctggaat atcctaaaac
    20581 gtaaaggaag atattacaag aaataacttc accgaattta ttgactaatt tgaggtcttc
    20641 tttccaatca acttgagcag ataagccagg agttgtttca acacgaatcg ttgctttttg
    20701 tattttatct gctttgatat tgcgacaata tcttctcagt gttcgtgcac ttccacgata
    20761 gccacgttta gaaataaaat gataaatagc agaagaagaa caacctaaag ctaatttgtc
    20821 agcgataata tcttcaaaat cactaaccaa agtttttttg tttttattct tttgagcaag
    20881 taaaggtaat ttattgctta ttccagcttc ataataacgt tttactgtac gataatcgca
    20941 attgtattgt ttagctagag cagcataatt aggttttgta tgagtcataa tgaaaggtaa
    21001 cactccttca taaatatctt ttctcaatac actttcctcc ttgaataaaa taacacaagg
    21061 aaagcgtacc agatgacgca taaacctaca tttttatttg gccatttttc tacattttaa
    21121 cttggccatt tacaacaaag aataaaaaaa tcaattatag tgttgatatt cttttagtgg
    21181 ataatccaga taaaaacaag ttcgaaactg aaactaaaga tttaagaacg atcggttata
    21241 cttcttctga aaaagtttta acaaacaaag atatgacaaa taaccaagtc aactattcag
    21301 cagtagttag aacagtgaaa gaaagagaga ataataatcc ggtcaaagag ttcaatgaga
    21361 gtatcaatta taaatttact gatagcacta aaacaaaaac aggatatggt atatcagttg
    21421 atttagagcc aacatataga aatgaaattg ggaaacaaat agatatgaaa gtctatagtt
    21481 atccgatgaa tgaattgatt gacagcaaaa ttgacgaaat ctatcaaagt gggggaaaag
    21541 aaacagaagt taagatggat cgcttaaaga atgatggtaa agttgagtcg ggtattagta
    21601 ctcaaaatct ttcattccaa tttccacata tgaatatcga aagaaaaact ggaaatatat
    21661 atgctgacag tcaaagaaat gatgaatcta ttaagtatga attgatcgat ggcggtagaa
    21721 aattatatgt gcctatttgg tgtgatttgg gaaattattc tattgctatc aaaagtagta
    21781 actttggtgg taactatatc tcgttggata tgaaccagaa catagatgtt tatgcttata
    21841 tgtatgcaac gatagatagt gaaacaaaaa aagatgatga attactgctt gaacctgttt
    21901 atccagagtc tactctacca gatggttgga gcaaaaatga gattgattgg ctgaataaat
    21961 aagtttgaaa agtttttatc tatctgatag aattaaataa tgatataggg atggtgatag
    22021 aatgagggtt caaagctatg gtaacagtgt ctttgatgtt aaaagagaat ttcaagattc
    22081 tagaagaaaa accaaaaagc tgatagctta cttcattcta tcagctatcg ttttctatat
    22141 tgtcaagttt gttttggaat taaactcaat agtattcaca gatatggttc ataaaatgtt
    22201 aaatggagaa cctattaatt tcaactttga ttacatgctt gaaaaagcac aaagcaatca
    22261 aattgtggct tttatcgtga gtatctatta ttttattgaa aaatctattt tttctttaaa
    22321 agattttttc tttgaaatgc ttaccaaact aacaacgttt tacaataaaa tttcaaaaaa
    22381 ttaaggagga agtttgaatg ttagatcaaa atacagacag aagttggtgg atgattggtg
    22441 ccgtgatcgt aggtgccatc ttaatcggga cagctaaagt agcttttccc gaagtgttta
    22501 atatcgtcat tacatttttc aaaaacatga tccctacaaa cttaggatag ttccactaaa
    22561 aaattcacgt agtttgatgc gtagaaaaat aataaagaat gaaaaaggct acataaagtg
    22621 atttatgtag cctttttttt ggatggaagt gatgtaatgc taagagatat gcccaaaatg
    22681 attcttttaa tgtttgttgg gttcttttta attttagtga ttgcgtacta ttcattaaat
    22741 ggctatacac aagattcgtc tgtgaaaggt atgcaagaaa cgttaagaac tacagcaata
    22801 gcaaacaggg atgatagtat tcgagtagaa aaaggacgat ttgctttaat taagtctgat
    22861 tttgaaaaag attttaaaga aaaatttggg aaaagtaaaa atctaaataa tgaaaccgct
    22921 gattataaat ttgattatct tacagatggg aaggacggta ttaaagcaat taaagtgaag
    22981 gttgaaagtg gatcaaaaga atatcaagca acttgtattt taaatgttgc aaatgattag
    23041 aggtgtgaaa tttgcgtgtt gtagaggaaa agaagatttc tccaaaaatc gaggaaactc
    23101 ctaaaagtaa aattaatatc gcaagtatta ttaaaaaaaa tggaattaaa gaaaactctg
    23161 aagcgataga aggaaacctt gattttttaa ctaagctttt agaagaagat agtggaaatt
    23221 atgaaccacc aattgaagat gcaaaattaa atatgattcg tgatgattta accagaaact
    23281 attcccaaga agttttacaa gctttaactg atgatgaaat taggcgaaaa cttgtacaag
    23341 ttgttttgaa agaacatggt actttcttta aaaaagataa agaggttgca gaatatgtag
    23401 tacgtgaatg tattggaaca gggattattg agcgaattat gaccaacgat gaaattacag
    23461 atgttggttg gaatggctct caacttagca ttgaaactaa ctctaacaaa tggttgtttg
    23521 acggtgataa attaaatatc acgcaagagt atattgaacg tgttatttct aaatatgctg
    23581 ttgtaaatga tcgggctttt aatagtggta atcctatcat ggatggaatg tttaaaaatg
    23641 ttcgtatatc agctacacac atagataatt caccagatgg ctctacgttt tctatgcgtg
    23701 tggcaaggcc tagattagca ttgaataaag aaaactttga agattttgct cctgattata
    23761 ttttggagct atttgagaaa atgatgaaaa caaaagcaaa tatctgtata tcaggggaga
    23821 caggtacagg taaaacggaa cttcaaaaat tactgctaag ttttattgat aatcatgatc
    23881 gtatgattat gattgaggac gttcaagaaa ctcacgctaa agaattattt ccggataaag
    23941 atattttctc ttggatcact actcccaaaa aagcaatttc agatttagta aaagcttctc
    24001 ttagaaataa tccacgatgg atcattgttt cagaaacacg tggttctgaa gcttatgaaa
    24061 tgcttcaagc ggttttgtca ggtcactatg ttgtaacgac acttcatgcg gttaatgcac
    24121 gtgcgatacc tagacgtttt gtaaatatgt gtgcaagtgg ttttaaaata aacgaaaatg
    24181 cggttgagga agacataaat cgttatttcg attttggtgt ccatatcaaa aaaacagtgg
    24241 ttgatggtaa agtgatccgt tatttgaatg aaattgttga attttctgct agtgaaccga
    24301 ctacagtttt taagcaaaaa tttatcaatg gaaagtttgt cattgaaaat ggtagtcttt
    24361 ccgaagattt taaagaaaaa atggctgaaa aatttattac gtttgaattt cctgaagatc
    24421 aaactaatac agaagaatca cttcctgaag aagaagaggt gatttaagtt gaaagtacag
    24481 gctatgacaa agcgaaaagg tctcatggat gtttcggaac taaacgccta tcaagtagcg
    24541 tatggtaaac cgttagatta tagagattat ttgaactatg ttggattacc ttgtttgttt
    24601 atgggtggct tttcttttgc tttatgttac tactggtgga ttgctttgat ttgtggagta
    24661 attggaggcg tatatggctt taaagttata atgcctaaaa gtattaaacg ctcttatgaa
    24721 ttaaagagtt tacaggaaag aaataaattt attaacaaca tgactcaaat tttgaccgat
    24781 gatagtaaaa cagttactag agcgttatct atagcaaaac agcgaacaaa aggtgaatta
    24841 agagaagata ttcaaatctt agaatcacgt ttacaaggtg ctgataaatt tcagattgct
    24901 gaagcttttc gtgaaatcaa caataaatat tatagtgatg ttgtttttac acaatacttt
    24961 gagcaattgg aaacagctat ttacgaaggt agaaataatt cagatactat gaagcaaata
    25021 aagagctatc ataatgatgt gaaacgtaaa acagaaggtt ttatgaaaat caaagacggc
    25081 tatcttcaag atatgaagca aatgatgttt attgtagctg tttttattgg agctataact
    25141 tttgcttttg gtttttctac tttctatgaa ggttttgctc gatcacctat cggatgggta
    25201 tttggatcta tttacttttt gattatggtt agcttcatga agtcattttt caaacattat
    25261 tttgatgatg aaataatgag tttgggggta agtaattaat gaaagttaag gctttaaaaa
    25321 agaaaagaaa atcaagtatt agccccttga aaattacgaa aacagaacat aaaagctttt
    25381 ggattaaatt actaggtgat gtttattatc aaaaattacg tgaaatgaac aattccaata
    25441 ttgcgattat taagtggcaa aaacaacggt taaaaaagtc attgattttt atcctagtcg
    25501 gattcttaat gtatctattt ttagggaaag aatggtatta cttagccggt ggaattgcta
    25561 cttcagtagg tttttatgtt tggcaagcaa aatcaatcaa tggtatctat aatcaatttc
    25621 ggtttgaacg acacttgcaa tttagtaaat ttacacggct attgatcccc tatctgaaac
    25681 aaaaaaaaga tggcggaaat ttatatggtg tttttgcaaa aatcgtaaaa agactagatt
    25741 atgataccga taaaaagcta ttaatgaagc ttatgcaaga aatgacagat cagcctaacg
    25801 atattagacc ttttacggaa tatgcagaac agacttctgg aacagacatg tccgtattat
    25861 ttatgtctac agtttatgat attcgacaag gttcggcaga tttggatgtt attgaagagt
    25921 tggatcgttt agcttctgaa gagttaatgg ccggaatcga ttcaatcatt gactataagt
    25981 cgaggcgttt tgtcttcttt cctacaaaaa ttgcaatgac ttcatttatt ttagttgtcg
    26041 gttttgcagt aggcgttctt ttcatgaacc taaaagatat taactttggg caataaagga
    26101 aaggagaaag tcaatgcaag aacaatccgt tatggaaatc attaaatggt ttataggttt
    26161 aatgatgatt atggcaatgg tttctatagg acttttttgt gttcaattac aagatattaa
    26221 tactttcaag caacaagtaa attatcaaat tgaacgaaaa ggaggtctga cgacagaagc
    26281 tgttgaagca atcgacaaat attcggctac taattatgat agacgtttta caattgaaag
    26341 cgataagtta aatcagaaag tatcttatgg agaagaaatt gattatacga tcaaagcaac
    26401 tattccaatt caaattttac cgcttcctga tgtaacttta tcgtttaacg gaaatagtgt
    26461 ttctcaaata agatgagagg aaggataaaa tgcaagagaa taatgtttcc agaataattt
    26521 atttgattgg cggattgata attttatcct tagctattgc cggtcttgta gagcattggc
    26581 cggaaataaa gttattaata agaaactttt acgacaataa aatgattaaa atgtaggagg
    26641 tatagatttg gaagcaattc aagaaaatcg taaggataat caagaaacaa gtgaaaaaaa
    26701 caaaaaaaaa agaaaaaaga aagtagataa atttacggaa atgaccgata aaatcattga
    26761 aaagaaagga cttaaaccta aagactttga ccgtcaagca atcaaagaag cacgacaaaa
    26821 actgtatgat gattttgtta aagaaaacat atcacagctt ttagactaa
//
LOCUS       DACEKQ010000034        26832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEKQ010000034 DACEKQ010000000
VERSION     DACEKQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.036"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(717..3866)
                     /locus_tag="IW671_001285"
     CDS             complement(717..3866)
                     /locus_tag="IW671_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0898277.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5124)
                     /locus_tag="IW671_001286"
     CDS             complement(3880..5124)
                     /locus_tag="IW671_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0898278.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5117..6709)
                     /locus_tag="IW671_001287"
     CDS             complement(5117..6709)
                     /locus_tag="IW671_001287"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0898279.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7218..7943)
                     /locus_tag="IW671_001288"
     CDS             complement(7218..7943)
                     /locus_tag="IW671_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898280.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8097..8423)
                     /locus_tag="IW671_001289"
     CDS             complement(8097..8423)
                     /locus_tag="IW671_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898281.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8460..8825)
                     /locus_tag="IW671_001290"
     CDS             complement(8460..8825)
                     /locus_tag="IW671_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0898282.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8862..9077)
                     /locus_tag="IW671_001291"
     CDS             complement(8862..9077)
                     /locus_tag="IW671_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898283.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9206..9541
                     /locus_tag="IW671_001292"
     CDS             9206..9541
                     /locus_tag="IW671_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898284.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9617..11614)
                     /locus_tag="IW671_001293"
     CDS             complement(9617..11614)
                     /locus_tag="IW671_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898285.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11712..11987)
                     /locus_tag="IW671_001294"
     CDS             complement(11712..11987)
                     /locus_tag="IW671_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898286.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12219..12953
                     /locus_tag="IW671_001295"
     CDS             12219..12953
                     /locus_tag="IW671_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898287.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12992..14134
                     /locus_tag="IW671_001296"
     CDS             12992..14134
                     /locus_tag="IW671_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0898288.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="IW671_001297"
     CDS             complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="IW671_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0898289.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15625..16095)
                     /locus_tag="IW671_001298"
     CDS             complement(15625..16095)
                     /locus_tag="IW671_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0898290.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16082..17317)
                     /locus_tag="IW671_001299"
     CDS             complement(16082..17317)
                     /locus_tag="IW671_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0898291.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="IW671_001300"
     CDS             complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="IW671_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0898292.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="IW671_001301"
     CDS             complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="IW671_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0898293.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19583..20431)
                     /locus_tag="IW671_001302"
     CDS             complement(19583..20431)
                     /locus_tag="IW671_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898294.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20455..21141)
                     /locus_tag="IW671_001303"
     CDS             complement(20455..21141)
                     /locus_tag="IW671_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898295.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21134..22168)
                     /locus_tag="IW671_001304"
     CDS             complement(21134..22168)
                     /locus_tag="IW671_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898296.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22563..22901)
                     /locus_tag="IW671_001305"
     CDS             complement(22563..22901)
                     /locus_tag="IW671_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0898297.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22903..23259)
                     /locus_tag="IW671_001306"
     CDS             complement(22903..23259)
                     /locus_tag="IW671_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0898298.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23265..24452)
                     /locus_tag="IW671_001307"
     CDS             complement(23265..24452)
                     /locus_tag="IW671_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0898299.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24643..25488)
                     /locus_tag="IW671_001308"
     CDS             complement(24643..25488)
                     /locus_tag="IW671_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0898300.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25722..26687
                     /locus_tag="IW671_001309"
     CDS             25722..26687
                     /locus_tag="IW671_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0898301.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7689 a         5549 c         4513 g         9081 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtattata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccctccc taaacaaaca tcttttgtaa
     3901 gaagcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtgtc
     3961 gtcgagttgt ttgaagaaag cgccgatttt ttgttgttcc tctttattcc tagtaaaagg
     4021 aaacttgata ttagcgaaat tctcatagaa taagtattct cgtacgcttc cttcagtgtt
     4081 gcgcttgacc tctttgataa atgttggtga cttgataaac tgaagtatga actcattatc
     4141 taagtcctcg ctcattttca caactacata aagagagcta acaatgaccg tcataccaag
     4201 attgttaaat gcaattgatc ctacgtttac tcttgctgga ttataggcga attcatttgg
     4261 ttcaacgaat ttataatttg ttttttctaa atcatccaac ctactaccat caaactgttc
     4321 tgtttgagaa attaaacctg ctttattact taccgaatag gcagggaagt ctaaatcttt
     4381 cgtgttcttt tttccagtct tcttactaaa ctctttgaac ttacgctgtt cccaatcttc
     4441 cgtaaatcca ggaaaccgaa tttctggcac ttttgctccg tttttaggga acattttttg
     4501 taagaatcct tttttcgttt cctttaataa atctagctta cgttgttgaa gagcgatagt
     4561 gtcgtccagt tgtttgaaga agttcccaat tttcttttgt tcttctaata ttggaaattg
     4621 tactatccat tttttcactt ctgacattgg tacgagattt gtaattgctg aacctggatt
     4681 agctcctgtg agttttcttt gcataaaatc tgcacgaata aattgaataa aatattctga
     4741 atctgtgatt tctttattta ttctaagtct aatcaaagcc tgattgaata cacccggttt
     4801 tatttgcttt ggtactcttg aaattctccc aattgttcca gcgccactca tgataaaatc
     4861 gcctggttct aatttaaatt tgattaattc attatatttt ttttcagtaa ttttatatct
     4921 tgtttcgtaa ttatcataaa tagcattttg ttgttcatag acaacaaaat tgctattaga
     4981 tacaaagaat tctttcttta aggcactccc aaatggtccc ctccttactc catctttttt
     5041 atcaagtaag cattcaaact tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat
     5101 ttctggttgt atatcattac tcatgtttga acacctccag cgcaccttgt aaccacgctt
     5161 cattttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt
     5221 gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt
     5281 cttcttcaaa cgtatctaca tagcgtggaa tatttaagtt atagtcattt tctttgattt
     5341 catcaaagtt tgccaaatga gcgtatttct cgacatcttt tctttcagca taggtttcga
     5401 ggattttttg aatgttttct tccgataatt tattctgatt ttttcctttg acaaattctc
     5461 gactagcgtc aatgaataat acgtctcgag tttggcggtt ctttttcaat acaatcaccg
     5521 tcgttggaat cgatgtcccg aagaacaagt tcgcaggcat accgatcaca gcataaatac
     5581 tgccgtcttc tagtaatttt tgacgaatga ccccttctgc agcgccacgg aataaaactc
     5641 cgtgtggcaa gacgatggcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa
     5701 gaaacgcaaa atctgctttg gattttggcg ctaattttcc ataacgatta aaccgagaat
     5761 cgtctaagaa ggtcgtatct gctgaccaat tagccgaata aggtgggttc attactactg
     5821 catcaaatgt gtaaggttca tctgttggcc aatccttatt caacgtatcc ccattgcgga
     5881 gattcatttc ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg
     5941 tcgttgtatt taattcttgc ccatgatatt taacgttatc tgggtgagtt aagtagttac
     6001 gcacatttaa catcaatgaa cctgaaccca tggttggatc aaaaacacta aagaatcgtc
     6061 gttctttttg atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt
     6121 aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcataag
     6181 catccccgat cacgtcccca tcgtgaccta aaacttccac gtcattcaat ttcttaatga
     6241 cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat
     6301 ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt
     6361 tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa
     6421 gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt
     6481 ttgattcttc atctgaaagt aattcgcgat acaacatcgt ttgtttgctt accgtatcat
     6541 attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat
     6601 atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc attttgctac
     6661 ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact
     6721 cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa
     6781 aagcaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac
     6841 gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc
     6901 tttagaacca tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag
     6961 tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac
     7021 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
     7081 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
     7141 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
     7201 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
     7261 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
     7321 atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa
     7381 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
     7441 tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta
     7501 agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct
     7561 tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa
     7621 gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta
     7681 ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc
     7741 ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag
     7801 tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt
     7861 aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc
     7921 cataataatt ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa
     7981 tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt
     8041 agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa
     8101 tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag
     8161 tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg
     8221 ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt
     8281 tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc
     8341 attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc
     8401 ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat
     8461 cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc
     8521 atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca
     8581 acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata
     8641 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     8701 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat
     8761 gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa
     8821 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta
     8881 aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt
     8941 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     9001 ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga
     9061 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     9121 ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta
     9181 ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga
     9241 aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca
     9301 acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc
     9361 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta
     9421 tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat
     9481 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     9541 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     9601 gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc
     9661 catcatctct tgttacactt gtaaattgat aaataggaac taattcaact cctgataata
     9721 tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac
     9781 tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     9841 cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga
     9901 catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc
     9961 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
    10021 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
    10081 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
    10141 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
    10201 agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta
    10261 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
    10321 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
    10381 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
    10441 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
    10501 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
    10561 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
    10621 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
    10681 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
    10741 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
    10801 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
    10861 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
    10921 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
    10981 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
    11041 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
    11101 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
    11161 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
    11221 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
    11281 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
    11341 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
    11401 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
    11461 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
    11521 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta
    11581 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
    11641 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
    11701 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
    11761 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
    11821 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
    11881 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
    11941 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    12001 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    12061 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    12121 cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc
    12181 ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    12241 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    12301 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa
    12361 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    12421 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    12481 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    12541 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    12601 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    12661 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    12721 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    12781 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    12841 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    12901 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    12961 tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa
    13021 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    13081 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    13141 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    13201 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    13261 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    13321 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    13381 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    13441 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    13501 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    13561 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    13621 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    13681 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    13741 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    13801 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    13861 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    13921 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    13981 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    14041 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    14101 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    14161 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    14221 taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact
    14281 gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca
    14341 gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt
    14401 tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg
    14461 atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc
    14521 gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct
    14581 tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt
    14641 gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt
    14701 gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt
    14761 ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg
    14821 tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta
    14881 aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct
    14941 tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc
    15001 gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt
    15061 ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg
    15121 tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa
    15181 gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg
    15241 tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct
    15301 ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga
    15361 gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg
    15421 tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat
    15481 tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc
    15541 aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct
    15601 tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc
    15661 ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat
    15721 ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc
    15781 tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc
    15841 tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca
    15901 gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc
    15961 gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct
    16021 tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa
    16081 tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt
    16141 cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa
    16201 ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt
    16261 ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg
    16321 tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg
    16381 gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt
    16441 gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt
    16501 cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt
    16561 cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac
    16621 caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg
    16681 cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc
    16741 cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg
    16801 atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca
    16861 tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg
    16921 ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt
    16981 ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg
    17041 taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt
    17101 ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa
    17161 cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt
    17221 gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga
    17281 taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac
    17341 ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa
    17401 tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta
    17461 catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc
    17521 atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg
    17581 actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa
    17641 ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg
    17701 ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac
    17761 gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac
    17821 atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc
    17881 acggcgattc atataagaag taatattttc tcctaattga tctacggctg aatatttgat
    17941 ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg
    18001 ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat
    18061 caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag
    18121 tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc
    18181 tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc
    18241 ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt
    18301 ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac
    18361 aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt
    18421 ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg
    18481 cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc
    18541 tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat
    18601 tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc
    18661 agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg
    18721 gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg
    18781 ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac
    18841 tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc
    18901 taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt
    18961 cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt
    19021 tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc
    19081 acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa
    19141 taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa
    19201 aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga
    19261 ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt
    19321 tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac
    19381 agacatgcat tataccccca aattctcttt cgttctcact cattttagac taattgagaa
    19441 tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt
    19501 tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt
    19561 ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta acagagccgc
    19621 cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt
    19681 tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt
    19741 tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt
    19801 ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg
    19861 ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg
    19921 ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga
    19981 tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt
    20041 cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg
    20101 cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga
    20161 agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga
    20221 atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa
    20281 tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg
    20341 ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg
    20401 ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct
    20461 tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa
    20521 aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca
    20581 agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat
    20641 cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac
    20701 cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg
    20761 gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac
    20821 aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg
    20881 aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga
    20941 tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat
    21001 caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag
    21061 tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt
    21121 ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga
    21181 acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa
    21241 aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc
    21301 gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg
    21361 ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt
    21421 ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga
    21481 cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc
    21541 agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct
    21601 tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag
    21661 tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc
    21721 gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga
    21781 ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt
    21841 ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga
    21901 agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat
    21961 cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga
    22021 ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa
    22081 cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt
    22141 tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat gatttttcaa
    22201 acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt
    22261 accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt
    22321 ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct
    22381 tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc
    22441 tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga
    22501 ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga
    22561 ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc
    22621 atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa
    22681 actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc
    22741 agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct
    22801 ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag
    22861 atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc
    22921 ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg
    22981 cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc
    23041 tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg
    23101 gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt
    23161 gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt ttgctttttt
    23221 gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat
    23281 cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc
    23341 cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt
    23401 aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg
    23461 cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag
    23521 attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg
    23581 ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat
    23641 cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc
    23701 attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat
    23761 ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt
    23821 cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca
    23881 gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct
    23941 tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa
    24001 aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc
    24061 atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat
    24121 cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag
    24181 agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg
    24241 gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc
    24301 atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat
    24361 gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga
    24421 ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac
    24481 tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa
    24541 aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta
    24601 tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc tgagattctc
    24661 ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag
    24721 cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa
    24781 cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc
    24841 ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat
    24901 attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac
    24961 aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta
    25021 tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt
    25081 cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg
    25141 aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga
    25201 agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc
    25261 ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg
    25321 aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg
    25381 tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa
    25441 atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc
    25501 ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct
    25561 ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat
    25621 cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc
    25681 ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa
    25741 ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta
    25801 aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga
    25861 tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc
    25921 caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt
    25981 ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct
    26041 tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa
    26101 ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa
    26161 ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct
    26221 atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc
    26281 cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc
    26341 ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag
    26401 tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa
    26461 gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc
    26521 attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat
    26581 gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta
    26641 aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat
    26701 aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt
    26761 catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc
    26821 aagaggtcgt aa
//
LOCUS       DACEKQ010000035        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEKQ010000035 DACEKQ010000000
VERSION     DACEKQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.037"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW671_001310"
     CDS             complement(98..1228)
                     /locus_tag="IW671_001310"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0898302.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW671_001311"
     CDS             complement(1877..3079)
                     /locus_tag="IW671_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0898303.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW671_001312"
     CDS             complement(3139..3342)
                     /locus_tag="IW671_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0898304.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW671_001313"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW671_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW671_001314"
     CDS             4879..5691
                     /locus_tag="IW671_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898305.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IW671_001315"
     CDS             complement(6715..6867)
                     /locus_tag="IW671_001315"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898306.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW671_001316"
     CDS             complement(7058..7936)
                     /locus_tag="IW671_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0898307.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW671_001317"
     CDS             complement(7975..9093)
                     /locus_tag="IW671_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0898308.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW671_001318"
     CDS             complement(9090..11189)
                     /locus_tag="IW671_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0898309.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW671_001319"
     CDS             complement(11240..12061)
                     /locus_tag="IW671_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0898310.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW671_001320"
     CDS             complement(12058..12867)
                     /locus_tag="IW671_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0898311.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW671_001321"
     CDS             complement(12888..13382)
                     /locus_tag="IW671_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0898312.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW671_001322"
     CDS             complement(13394..13882)
                     /locus_tag="IW671_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0898313.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IW671_001323"
     CDS             complement(13959..16655)
                     /locus_tag="IW671_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898314.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IW671_001324"
     CDS             complement(17083..18156)
                     /locus_tag="IW671_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898315.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IW671_001325"
     CDS             complement(18153..18980)
                     /locus_tag="IW671_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898316.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IW671_001326"
     CDS             complement(18981..19787)
                     /locus_tag="IW671_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898317.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IW671_001327"
     CDS             complement(19799..20884)
                     /locus_tag="IW671_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898318.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IW671_001328"
     CDS             complement(20901..21443)
                     /locus_tag="IW671_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898319.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IW671_001329"
     CDS             21701..22690
                     /locus_tag="IW671_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0898320.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IW671_001330"
     CDS             22687..23424
                     /locus_tag="IW671_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0898321.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IW671_001331"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IW671_001331"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0898322.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IW671_001332"
     CDS             23988..24620
                     /locus_tag="IW671_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0898323.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IW671_001333"
     CDS             24705..25352
                     /locus_tag="IW671_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0898324.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IW671_001334"
                     /pseudo
     CDS             25357..26340
                     /locus_tag="IW671_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
BASE COUNT         8060 a         5570 c         4464 g         8258 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagctgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgta
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactagc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg at
//
LOCUS       DACEKQ010000036        25973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEKQ010000036 DACEKQ010000000
VERSION     DACEKQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.038"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1273..1938)
                     /locus_tag="IW671_001335"
     CDS             complement(1273..1938)
                     /locus_tag="IW671_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898325.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IW671_001336"
     CDS             2064..4100
                     /locus_tag="IW671_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0898326.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IW671_001337"
     CDS             4104..4742
                     /locus_tag="IW671_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898327.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IW671_001338"
     CDS             4770..5198
                     /locus_tag="IW671_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898328.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IW671_001339"
     CDS             5655..6566
                     /locus_tag="IW671_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0898329.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IW671_001340"
     CDS             6783..7220
                     /locus_tag="IW671_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898330.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IW671_001341"
     CDS             7315..8199
                     /locus_tag="IW671_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0898331.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IW671_001342"
     CDS             8423..11590
                     /locus_tag="IW671_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898332.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IW671_001343"
     CDS             11610..12155
                     /locus_tag="IW671_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0898333.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IW671_001344"
     CDS             12183..12638
                     /locus_tag="IW671_001344"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0898334.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IW671_001345"
     CDS             12635..13393
                     /locus_tag="IW671_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0898335.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IW671_001346"
     CDS             complement(13479..14219)
                     /locus_tag="IW671_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0898336.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IW671_001347"
     CDS             14280..15533
                     /locus_tag="IW671_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0898337.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IW671_001348"
     CDS             15855..16082
                     /locus_tag="IW671_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0898338.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IW671_001349"
     CDS             16205..16531
                     /locus_tag="IW671_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0898339.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IW671_001350"
     CDS             16599..17312
                     /locus_tag="IW671_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0898340.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18473
                     /locus_tag="IW671_001351"
     CDS             17622..18473
                     /locus_tag="IW671_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898341.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18594..20459
                     /locus_tag="IW671_001352"
     CDS             18594..20459
                     /locus_tag="IW671_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898342.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20507..21916
                     /locus_tag="IW671_001353"
     CDS             20507..21916
                     /locus_tag="IW671_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0898343.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22024..22725
                     /locus_tag="IW671_001354"
     CDS             22024..22725
                     /locus_tag="IW671_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0898344.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22884..23363
                     /locus_tag="IW671_001355"
                     /pseudo
     CDS             22884..23363
                     /locus_tag="IW671_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24211..24786
                     /locus_tag="IW671_001356"
     CDS             24211..24786
                     /locus_tag="IW671_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0898345.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24786..25226
                     /locus_tag="IW671_001357"
     CDS             24786..25226
                     /locus_tag="IW671_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0898346.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25242..25943
                     /locus_tag="IW671_001358"
     CDS             25242..25943
                     /locus_tag="IW671_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0898347.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         8768 a         4222 c         5360 g         7623 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggcagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgtttt aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc
    17821 cggcagagga gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag
    17881 ttgaattttc gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac
    17941 gagcaaaaga aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc
    18001 cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg
    18061 aactttctga ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa
    18121 acgaagacat gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca
    18181 tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta
    18241 ctcatctgca gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg
    18301 aatttctcta cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga
    18361 tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt
    18421 taacgattca tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt
    18481 tactgtatgg caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt
    18541 tttttcaatc tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata
    18601 atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt
    18661 tagtacattg tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc
    18721 aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag
    18781 tagtgattgg cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg
    18841 cagtttcgga ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta
    18901 tctctagtat atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat
    18961 tcttatcatt ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat
    19021 tgtttgctgc agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag
    19081 ctaaaaagtt caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc
    19141 cagctatcac agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga
    19201 ttctttcacc atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc
    19261 aaagtaaatt agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag
    19321 ttccgcttgt cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg
    19381 ggacggttgc cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa
    19441 tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt
    19501 ggggattcgt accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc
    19561 caatgcttct tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa
    19621 caaaagatac gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa
    19681 tcactgaacc aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt
    19741 gtatttctgg aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca
    19801 gcagtttggt cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt
    19861 caaatgtgac agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta
    19921 cattgatttt aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga
    19981 atgcaggaga accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag
    20041 tactgccatt gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta
    20101 tcgctattga tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta
    20161 tttttccaac tggacatgct gtagggatca caacgactga cggagcggag attttgattc
    20221 atatcgggat ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag
    20281 gggatctcgt caaagcagga gatttattga ttcgttttga catcgaagca attagagcag
    20341 ctggctatag cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac
    20401 ttgaattaga ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag
    20461 gaggaaatca ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct
    20521 gccaaatcaa ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc
    20581 tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat
    20641 tgatcaagaa aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa
    20701 agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg
    20761 gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta
    20821 tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca
    20881 ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat
    20941 cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata
    21001 ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt
    21061 agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt
    21121 cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg
    21181 ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc
    21241 aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga
    21301 ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga
    21361 gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag
    21421 ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa
    21481 cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga
    21541 tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt
    21601 cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga
    21661 tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga
    21721 cggtgtagat ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac
    21781 aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg
    21841 gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa
    21901 tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt
    21961 tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac
    22021 gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt
    22081 ttctcaggaa aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg
    22141 tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat
    22201 gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta
    22261 tccaaagcgg caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa
    22321 acgctggatt tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt
    22381 gagcaagctg tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt
    22441 gttcattatc ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct
    22501 ttgtatcgtt tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg
    22561 gcagtatttt taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc
    22621 aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc
    22681 taccatacgg agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt
    22741 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    22801 ttttcgtttg gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt
    22861 ctggataagg gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga
    22921 ttttagcttc gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca
    22981 ttcgtaaaaa tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg
    23041 ggatctgtca gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg
    23101 tcatagcaac gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag
    23161 atagccgtat tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat
    23221 tcattggtgc attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa
    23281 aaccagtaga tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt
    23341 gggatgattt gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag
    23401 atgtaccttg agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag
    23461 attttggttt aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga
    23521 tgaaggaaaa atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga
    23581 aaggaaacaa aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta
    23641 aaaaaagaaa ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac
    23701 tagttccagg caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat
    23761 tcaagtgata tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag
    23821 ctgatgaaca aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga
    23881 aacatcattt atcagtgaaa cataacacga gaacaaaatg attttttcgt taataaaaga
    23941 aacaagtatt tgttgtttaa aataatacac gttattggca tataggacga aatcccagta
    24001 tcttctataa gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct
    24061 ataaaatggc tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata
    24121 taccactaaa aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag
    24181 atagactttt gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt
    24241 tttggtaata aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa
    24301 gaagaaatcg aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa
    24361 acagtactgg aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact
    24421 gaattgcctg gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag
    24481 gtattattat ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg
    24541 gaggaattct cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc
    24601 ggaatattga atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca
    24661 aaagaggatt ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg
    24721 gaactggtca atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca
    24781 aaataatgag tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag
    24841 taagtcgtat cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac
    24901 gtcatcacca agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg
    24961 aaaaagatat ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc
    25021 atcaaacaat cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac
    25081 cgatgatcat tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc
    25141 atacgtattt aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg
    25201 aaacttacgt aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt
    25261 caggaacgat tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt
    25321 ctaaaccatt ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc
    25381 ctgagcagta cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga
    25441 aaaatgcttt agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt
    25501 tgttccatct aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata
    25561 gcttattaga tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt
    25621 tgatggtcca tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc
    25681 gtgactatga acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca
    25741 cccgctataa tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg
    25801 ataaattcga ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag
    25861 aaaaattagc tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg
    25921 caaaaggtca agaattaggc taaaaataaa aaaccaaacg acgggaaaga aaa
//
LOCUS       DACEKQ010000037        24391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEKQ010000037 DACEKQ010000000
VERSION     DACEKQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.039"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1443)
                     /gene="nhaC"
                     /locus_tag="IW671_001359"
                     /pseudo
     CDS             complement(75..1443)
                     /gene="nhaC"
                     /locus_tag="IW671_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(1552..2973)
                     /locus_tag="IW671_001360"
     CDS             complement(1552..2973)
                     /locus_tag="IW671_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0898348.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IW671_001361"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IW671_001361"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0898349.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="IW671_001362"
     CDS             complement(5384..6640)
                     /locus_tag="IW671_001362"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0898350.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="IW671_001363"
     CDS             7220..8521
                     /locus_tag="IW671_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0898351.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="IW671_001364"
     CDS             complement(8590..9189)
                     /locus_tag="IW671_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0898352.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="IW671_001365"
     CDS             9443..10324
                     /locus_tag="IW671_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898353.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="IW671_001366"
     CDS             10337..11947
                     /locus_tag="IW671_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0898354.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="IW671_001367"
     CDS             complement(12024..12683)
                     /locus_tag="IW671_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0898355.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="IW671_001368"
     CDS             complement(12966..13631)
                     /locus_tag="IW671_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898356.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IW671_001369"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IW671_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0898357.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IW671_001370"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IW671_001370"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0898358.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IW671_001371"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IW671_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0898359.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="IW671_001372"
     CDS             15945..16265
                     /locus_tag="IW671_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0898360.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="IW671_001373"
     CDS             complement(16377..17246)
                     /locus_tag="IW671_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0898361.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18646)
                     /locus_tag="IW671_001374"
     CDS             complement(17342..18646)
                     /locus_tag="IW671_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0898362.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18643..19152)
                     /locus_tag="IW671_001375"
     CDS             complement(18643..19152)
                     /locus_tag="IW671_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898363.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19327..21462)
                     /locus_tag="IW671_001376"
     CDS             complement(19327..21462)
                     /locus_tag="IW671_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0898364.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21545..23200)
                     /locus_tag="IW671_001377"
     CDS             complement(21545..23200)
                     /locus_tag="IW671_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0898365.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23202..23516)
                     /locus_tag="IW671_001378"
     CDS             complement(23202..23516)
                     /locus_tag="IW671_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0898366.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23686..23946
                     /locus_tag="IW671_001379"
     CDS             23686..23946
                     /locus_tag="IW671_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0898367.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23960..24274
                     /locus_tag="IW671_001380"
     CDS             23960..24274
                     /locus_tag="IW671_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0898368.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6946 a         5042 c         4402 g         8001 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc caaataaaat
      721 aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt tttggatcat
      781 ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg tataaaaaat
      841 caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat gttcgaatag
      901 attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg agcagttatt
      961 ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga atccaagtaa
     1021 atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg aactgccgac
     1081 agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac
     1141 aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc atgaagcaac
     1201 taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga ttcctttgat
     1261 gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa acaacaaaca
     1321 catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga tcagtactcc
     1381 taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa tatttttatt
     1441 catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta ttcacttggc
     1501 aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
     9301 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag ttgtgatcat acgcgaagga acactcgatg aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agttgtacga tgattttttt
    10801 ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
    18601 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
    18661 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
    18721 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
    18781 aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt
    18841 tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa
    18901 aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc
    18961 gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa
    19021 aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca
    19081 gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt
    19141 tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat
    19201 ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt
    19261 ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc
    19321 tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt
    19381 gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac
    19441 atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc
    19501 atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat
    19561 aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc
    19621 ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat
    19681 gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga
    19741 acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg
    19801 cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa
    19861 agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc
    19921 ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa
    19981 atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata
    20041 agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc
    20101 catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata
    20161 agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct
    20221 atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag
    20281 atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt
    20341 aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt
    20401 tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg
    20461 ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc
    20521 tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga
    20581 aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt
    20641 cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat
    20701 cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc
    20761 acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt
    20821 ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa
    20881 gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg
    20941 atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg
    21001 aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg
    21061 ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa
    21121 ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt
    21181 caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg
    21241 atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact
    21301 gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt
    21361 tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt
    21421 cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt
    21481 ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg
    21541 agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg
    21601 caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc
    21661 tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc
    21721 ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt
    21781 cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat
    21841 gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt
    21901 ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat
    21961 cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac
    22021 cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact
    22081 tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa
    22141 tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg
    22201 cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa
    22261 ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa
    22321 taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa
    22381 tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt
    22441 cgattccagc ggtcactgct agctggttat caggacgaac accaatggat aaaataacta
    22501 aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc
    22561 cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa
    22621 aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga
    22681 cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa
    22741 cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta
    22801 catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg
    22861 gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat
    22921 gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca
    22981 catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt
    23041 cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc
    23101 ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag
    23161 acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt
    23221 cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc agatttcttt
    23281 aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt
    23341 tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc tccttttata
    23401 tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc
    23461 ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc
    23521 tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg agctttatta
    23581 atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac
    23641 cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat
    23701 aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg
    23761 atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt
    23821 attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct
    23881 gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa
    23941 aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc
    24001 agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt
    24061 atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga
    24121 tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga
    24181 aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact
    24241 gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat
    24301 cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca
    24361 aaggttgtga aaacgctgtc ctgcatcaag t
//
LOCUS       DACEKQ010000038        24205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEKQ010000038 DACEKQ010000000
VERSION     DACEKQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.040"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..1202)
                     /locus_tag="IW671_001381"
     CDS             complement(30..1202)
                     /locus_tag="IW671_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ0898369.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(2232..2843)
                     /locus_tag="IW671_001382"
     CDS             complement(2232..2843)
                     /locus_tag="IW671_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0898370.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            complement(2883..3665)
                     /locus_tag="IW671_001383"
     CDS             complement(2883..3665)
                     /locus_tag="IW671_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898371.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            complement(3679..5061)
                     /locus_tag="IW671_001384"
     CDS             complement(3679..5061)
                     /locus_tag="IW671_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898372.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRSNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLEE
                     YITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDSIIKNIFTKYY
                     SKEFEVTQIP"
     gene            complement(5110..6006)
                     /locus_tag="IW671_001385"
     CDS             complement(5110..6006)
                     /locus_tag="IW671_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898373.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            complement(6201..6743)
                     /locus_tag="IW671_001386"
     CDS             complement(6201..6743)
                     /locus_tag="IW671_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898374.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIA
                     ELSNKTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSNG"
     gene            6914..7102
                     /locus_tag="IW671_001387"
     CDS             6914..7102
                     /locus_tag="IW671_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0898375.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            7148..7525
                     /locus_tag="IW671_001388"
     CDS             7148..7525
                     /locus_tag="IW671_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ0898376.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(8085..9257)
                     /locus_tag="IW671_001389"
     CDS             complement(8085..9257)
                     /locus_tag="IW671_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898377.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(9501..10469)
                     /locus_tag="IW671_001390"
     CDS             complement(9501..10469)
                     /locus_tag="IW671_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0898378.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(10466..10663)
                     /locus_tag="IW671_001391"
     CDS             complement(10466..10663)
                     /locus_tag="IW671_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898379.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            complement(10674..10991)
                     /locus_tag="IW671_001392"
     CDS             complement(10674..10991)
                     /locus_tag="IW671_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898380.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            complement(11028..11246)
                     /locus_tag="IW671_001393"
     CDS             complement(11028..11246)
                     /locus_tag="IW671_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898381.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(11355..11546)
                     /locus_tag="IW671_001394"
     CDS             complement(11355..11546)
                     /locus_tag="IW671_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898382.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(11708..12064)
                     /locus_tag="IW671_001395"
     CDS             complement(11708..12064)
                     /locus_tag="IW671_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898383.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(12089..13093)
                     /locus_tag="IW671_001396"
     CDS             complement(12089..13093)
                     /locus_tag="IW671_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ0898384.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(13130..13390)
                     /locus_tag="IW671_001397"
     CDS             complement(13130..13390)
                     /locus_tag="IW671_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898385.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(13399..14472)
                     /locus_tag="IW671_001398"
     CDS             complement(13399..14472)
                     /locus_tag="IW671_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898386.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(14480..16306)
                     /locus_tag="IW671_001399"
     CDS             complement(14480..16306)
                     /locus_tag="IW671_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0898387.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(16461..17327)
                     /locus_tag="IW671_001400"
     CDS             complement(16461..17327)
                     /locus_tag="IW671_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898388.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(17502..17765)
                     /locus_tag="IW671_001401"
     CDS             complement(17502..17765)
                     /locus_tag="IW671_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898389.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(17767..18096)
                     /locus_tag="IW671_001402"
     CDS             complement(17767..18096)
                     /locus_tag="IW671_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898390.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(18118..19653)
                     /locus_tag="IW671_001403"
     CDS             complement(18118..19653)
                     /locus_tag="IW671_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898391.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(19720..20187)
                     /locus_tag="IW671_001404"
     CDS             complement(19720..20187)
                     /locus_tag="IW671_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898392.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(20184..20480)
                     /locus_tag="IW671_001405"
     CDS             complement(20184..20480)
                     /locus_tag="IW671_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898393.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(20831..21061)
                     /locus_tag="IW671_001406"
     CDS             complement(20831..21061)
                     /locus_tag="IW671_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898394.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(21097..21993)
                     /locus_tag="IW671_001407"
     CDS             complement(21097..21993)
                     /locus_tag="IW671_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898395.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            complement(22007..22657)
                     /locus_tag="IW671_001408"
     CDS             complement(22007..22657)
                     /locus_tag="IW671_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0898396.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            complement(22672..23808)
                     /locus_tag="IW671_001409"
     CDS             complement(22672..23808)
                     /locus_tag="IW671_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898397.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            complement(23830..>24205)
                     /locus_tag="IW671_001410"
     CDS             complement(23830..>24205)
                     /locus_tag="IW671_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ0898398.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
BASE COUNT         6976 a         4568 c         3019 g         9642 t
ORIGIN      
        1 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
       61 gaataaatcc attcatcatg taggtccata agaacggctc caattaagcg attggctgat
      121 gtttgattgg ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt
      181 tcaattagat tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag
      241 gcgtcttcga atccatcatc caatgatgcg caagcttttg aatattttgg ttgatcgata
      301 taatcatgaa tcaatcgatt tttagcctca cgcgctaagt taatatctgt gaacttaaaa
      361 attcctttaa cagcttctct gaaagatttt gaattttttt taggaatggt ggtaaagata
      421 tttcttagga agtgaacttg gcatctttgc caacttacgt tggtgaagga ttttctaatg
      481 gcagagacta atcctttgtg cgcatcagaa ataacgagtt ccgtaccttg taaaccgcgt
      541 tcttttaggt attcaaaaaa tgttgtccag gtctcttcgc tttcgccact ttgaatcatg
      601 aagccgataa tttcacggtc gccatctttg gttattccaa tcgctatatg acagcttttt
      661 gagagtactc gattttcttc tcgtactttt atatagagta catcggtcat taagtaagga
      721 taattttttt ctgataataa acgattctgc cactcgttaa ccataggttc tagctgttct
      781 gttaagctag aaacgaagga cttagagacg gatttaccac aaagttcttc cacaattttt
      841 gatactttac gagttgaaac gcctgataca tacatttcca acattgaagc catgagggct
      901 ttttcgtttc gttgataacg ttcaaacact gtgggtgaaa aatggccatc acgtgttctg
      961 ggtactttta attctagcgt gcctacacgt gtcgtaaagc tgcgctcata atagccattt
     1021 cgttgacttt gtcggttttc tgttcgttca tattcttttg cttgaatata ttctgttcgt
     1081 tgattttcca ttagttgatt aaataccgtt gttaaaatat ttttagaaac gtcatccttt
     1141 acagaatatt caataatgct ttgaatctct tcgcttttca gtgtaaaatg tacttgggtc
     1201 atgtaaaagt cctcctgggt atgtttttgt cgttaaaaac attgtaccgt aaaaggactg
     1261 ttatatggcc tttttacttt tacacaatta tacggacttt atctaattct accaatatta
     1321 ctattttcat tcttatgaat tagtaaatca tagacatcaa atacgataat acaaactatt
     1381 aagaacaata taaatattac cttattgtta tatacaaatt cagttgattt tcctattaag
     1441 tataaaacta aataaattag ctcccacata ttctcactct ttaattcgaa ttttcatata
     1501 cgcaattctc ctgtgattct tcgtagacct gtttaataga agctgattta cctaatccca
     1561 ttgcacctaa aatatactta ttattggcgc atgttttttg gtattccact gcttcacatt
     1621 cattctccat ttttttactt aatcaaatct ttcgtgctta ttatccaact ccatgatacg
     1681 cattatctct tgtgcatttg ctttactatt aaaaatcata aatggatatg tttctccgtc
     1741 tacataaatt ccccaatcgc agacaacttc tttgatctct agtttcataa aaaacacctt
     1801 cctgtagata atcaattatc actcatgctt atcaattgat tcttgaatgg tattgcaagg
     1861 aaatgtattg aatctgtttt cgtcatattc taacgttgta tactgtggcg taagagaact
     1921 attatcttca acacttattg aatcgaatat gcaacctaaa attctgacca gtcataattt
     1981 ttttcttctg tactttctgc tgtaggattt ccaaaaaatt tcagtcgaaa ttctttttca
     2041 aaatcattaa cccaattaac ttgttgattc actctcgcat agcctctttc ttttctattt
     2101 ctgtcgttac ttgactgcca caaaattaat tcttttgttt agaagcttct ttgtataatt
     2161 gtacacctta aaagtttgaa acaggcttag aatccttctc tcgtaacatt gatttctcaa
     2221 agaacttaaa atcaatcaga ttcacttatt cttttcttta ttctatagac taaatttcta
     2281 tttgttcctg ttttttctgc aattcgctta atcggaacac cttctccaag cattcttact
     2341 atttcattat agataaacct cttttgtgga ttctttgcag tagaagaata ttcgtagggt
     2401 tttcctttat agactccttt cttcttcgca attgctattc cttgtttttg ttttctctta
     2461 atttcctccc tctcattttg agccgtccaa gaaaaaatgt taattactaa gcttctaaca
     2521 agtttttcta agtttggatc accaaatttt gtatctaaaa ttggtaaatt caaaaccatt
     2581 aagcccatac catttttatc tataaaagat actgtttcta gtatttcatc agaatttctt
     2641 cctaagcgtt caagcgactc aaaaacaagc gtatcccctt ctctgacaaa tgacaacatt
     2701 ttattgaact ctggccgttc tactgtgtta cgtccagatt ttttctcagc aaatactttt
     2761 tcgacaccaa tttttctaaa ttcttcaagc tgtctttcca aattttgatc atctgaactg
     2821 actcgtgcat agccaactaa catatttaac acctacaaat aattttactc tagtttaatt
     2881 aactaggaat tttttcgtga gttcagcaga ctctctatac catcaaataa acattcatag
     2941 tattttattt tttcttttag ctttgaattt tcttctttga gttcatttat caatttaact
     3001 tgggattttt cagaaataaa gccttcatca aaaagcaaat cttctacact acaattcgca
     3061 atgtctgcaa ttttttttag tttggctttg ttcggtaagt taccaccatg ttcccatgtt
     3121 gacacagtgc ttctaggaga attagaaacc tcgtacccaa attcctgcat agtaagtcct
     3181 aatttttttc taatagaacg aatttttctc cctacctttt tagggtctgg tttaacttct
     3241 atgtctcgat aatcttcaaa atcttttgag taatattttg taaatatatt ttttattata
     3301 ggattcaata actcatagtc tttatttagc gaaaacgtat tactatatct ttcttgaatg
     3361 tctttaaaga ctttgtgagg tatttctgga aagtccttaa tatatagctc ataccctata
     3421 tcgataaggt atgaagttat atattcttct aaagttcccc atttaatcca gtcaattgtt
     3481 gaattagtga tagttgataa taatttcagc ttttgtggat ttggtaaatt ggtcccacgt
     3541 tcccagttgg atatggtcgc acgtggtgaa ttatctattt tccttccaag ttcttccata
     3601 gtaaaaccac atgaaaggcg taattgtttg attcgttttc ctgcttctga aggatttggc
     3661 ttcatacaat cacctctctt atggaatttg ggttacttca aattcttttg agtaatattt
     3721 tgtaaatata ttttttatta tagaatccaa taattcatag tctttatcta acgaaaacgt
     3781 atcactatat ttttcttgaa tgtctttaaa gactttgtga ggtatctctg gaaagtcttt
     3841 aatatatagc tcatatccta taccgataag gtatgaagtt atatactctt ctaaagttcc
     3901 ccatttaatc cagtcagtgg tggtttctcc tactatagca atcagctgta actttttctg
     3961 agaaggtata tttctaccac tttcccaagt agcaattgta cttttaggtg agttgcttaa
     4021 atgagaacca aattgctcca tagtcatacc taattttttt ctgatatatt tgattctttt
     4081 acttacgtct tttagattgg gaatcatttt ttcagctcct tgatttacta cttgttggat
     4141 ttcatacaat ctttttcgta agctttctac ctcatatttt aattcctcat tttcttgttt
     4201 aagaaggata ttttcggctg tcaaactttc atacggctct actatattat cagtagaaga
     4261 atcgttgatt aatgctacta tcggaacgtc caatgctctt gcaatagctt ccagtgtttt
     4321 tatactggga ttactcctgt tgttttctaa atcccctata tatgatcgtg atagacccat
     4381 taactcagca aaatcagttt gtgtcatatt tttacttttt ctaatatttt tgatgttgtt
     4441 tccaatatta aggtttttga gttcaacatt tgtttcgtct aatcccatca aataacttac
     4501 aggaacatgg aaataatcag caaggatatt ccatgtttca gtatttctag gatttcgctc
     4561 accacggtaa tagttcgtaa gagttgtata gttaatacca gtttctgtac taatttgttt
     4621 aatggtcttt ttactggaat caatataaaa ttttattcta ttcaaagtaa cgccccctat
     4681 agtaaatttt attataggat tcaataactc atagtcttta tctagtgaaa acgtatcact
     4741 atatttttct tgaatgtctt taaagacttt gtaaggtatc tctggaaagt ctttaatata
     4801 tagctcataa cctgaatcta taaggtatga agctatgtat tcttccaaag ttccccattt
     4861 aatccagtct acactagtgt ttccaatatt tgcaattgtt aaaagcctat ttttattggg
     4921 taaagacacg cctctaagcc aattattaac agaacttatt ggtgtgtcgc caattaaatc
     4981 accgaatcgt tctaatgtca gacctttgga ttcgataatt tttctaatac gttcactagt
     5041 taacgataga ttaggtttca tagctaagcc tcctcgtgag taaagtaatc tttcaatatt
     5101 ttttgtgaat caacccattt tatcgagaag tgatagttga caaatagcat tgagagatat
     5161 ttctcgctta tctggatcat caatttttga aatcacatta agataaccgg cgaagctttc
     5221 aattattcta ttgacttcct caattgacag acgttccaaa gcatccaatg ctacttcaga
     5281 tagaaaatac attgattctg ctacgcttga atcgacaccc gtcataagat aaagttcaga
     5341 aactccgcca atttctgcaa tctttttaat tctttctgag ttaggaaggt ttttaccatt
     5401 ttcccagttg cttataacag ctttatcagc tgtaggagag aatagtttgc cgaattcttc
     5461 taatgtaata ttctcttttt ttcttatttt ccttatacgt tgtccaatca ttttaatatt
     5521 tttgttgtca tcagtcagtc ctaaatctgg tttgacttct atatttcgat aatcttcatc
     5581 aggagaatac atgtacttca cctcctgaaa aatattttgt gtattgattt tttcggcagc
     5641 tgctaatatc tttgtatctt cttcgtatgt gattttttca atcgtaagtt catctagaag
     5701 agcttcacgg tagttgttca gtgtagtctc tccgtactct ttaagaattt tttcctctat
     5761 attttgtgtc aagcgataaa cataatcgga aaaatctcca tactttatcc attcaacaga
     5821 agtaccacct aatagtgcaa ttttttcaag acgctttcta ttaggtaagt tatttccctt
     5881 ttcccaattg ttaattgtgc taggactatt ggcatcaacc agtttaccta agtctgccat
     5941 agtatatcca tgtttttctc gtatttgctt tattcgatta ccagcctcaa aaagatttat
     6001 ttccattcga cacctcctaa tgtatatcat ataccgaaat catataaatc tatttcaatc
     6061 agatttacaa aacggtcatt cataaaaact agggattttt tcgtgagttc agcagactct
     6121 ctataccatc aaataaacat tcatagtatt ttatcttctc ttttaacttt gaattttctt
     6181 ctttgagtcc atctatcaat tcaaccgttt gagtaatatt ttgtaaatat attttttatt
     6241 atagaatcca ataattcata gtctttatct aacgaaaacg tatcactata tttttcttga
     6301 atgtctttaa agactttgtg aggtatctct ggaaagtctt taatatatag ctcatatcct
     6361 ataccgataa ggtatgaagt tatatattct tctaaagttc cccatttaat ccagtcgata
     6421 gttttgttac ttaattcagc aattttaatt aatctttctt tgttgggaag ggagaatcct
     6481 cgttcccaat tattaatatt tcctttttga gcatttccaa ttaaatctgc aaattgttcc
     6541 atagtcattc caaaattaga acgaattgat ttaattcttt tacctgtttc aacaggattt
     6601 ggtttcatac aatcacctct tttacagaat ttggtttcat agtatcttat tcgctctttg
     6661 agttctaagt tctctttctc caatttttct atataatatt cttgatcctg tttcttgttc
     6721 aaaaaatgac ctccaaattt cacattgtgt tacaccctag tgttacacct ccaaacctta
     6781 ttatactgta ctttaccata tgatacaata gggtataccc tattgtgaca cttacattct
     6841 acttttaatg taaaaactct tgacctttat acgcataatg cgtataataa tatttgtaag
     6901 gaggtagtcg cgaatgccac ttaccggtaa aaaaatgctc aaactagcca agaaaaatgg
     6961 ttggaaagtg gtgaggataa ctggcagcca tcatattgta gccaaagaag gattcccacc
     7021 cgttcctatt cctgttcata gtaatcagga tttaaaaaaa gggacagagc agaagttact
     7081 aaaggactta gggctgaaat agcccagtcc tttagttcat tggattaact cgaaaggagt
     7141 ggtggaaatg aaaaaaagtt accctgtaat tttttctgct gaagaaaatg gctactcagt
     7201 agtttttcct gattttgctg gagcaacaca aggagataat tttgaagaag ctatgtccaa
     7261 tgctaaagaa tttcttgatg ggattctagc ttactatatt gatgaagata tttcagttcc
     7321 ttctccaaca gatattcatc aactaaataa tttgggcaat aacaaaattg tttctcttat
     7381 tcaaagtgat ccttctccat ttatccaaaa aaagaccgta cgcaaaaacg tcacagtccc
     7441 agaatggtta gtagctagag gggaaaaaga taaaataaat ttttctaaag ttttgacaga
     7501 tgcgttacta aaaatatacg agtaaacgta aatatattaa taacaattta gttgcctaat
     7561 gacatagata gcggaattaa aagtaaaata gtactcattt attgcaggtt cccttagaat
     7621 caatacaata aatgagtact atttagactg tttaactact agtaagatat agcgatcatc
     7681 agtttcaggg ttatcccttg gtgaccatag ttttgcgtat ttttcaactc gttgccaaat
     7741 caacgattct atttttttat ttctacttaa ctccaaaacg gagtaccttc tttttcttcg
     7801 ttagctccta tttaagagcc atgtcgtttt atataactct aaaacagaaa ctactgatca
     7861 attcaataaa gccttatttg agaactgtgt tgttttacat ggttttaaaa cctgatttcc
     7921 cgcaaacggt ttatccgtaa catttgagaa ctgtgttgtt ttacatggtt ctaaaacctc
     7981 aacaatattc gtccttccgt ctggttacac aacttgacag agctaaccct tcagtgagtg
     8041 agtgtcccct cactatttcg ttgccaaatc aacgatttct tattctactc acttttaact
     8101 tctattccat atcttttccc tttatttatt atttgttctt tcaactgata ataagcccat
     8161 tccccataat ttttatctgg ttcagaaata cagattattt gcgtcttatt gcggatagct
     8221 gtttgaataa tttttgccga acattgatta ttataggttt taacaaaatt gctttctctt
     8281 ttctttatgg cttcccatct atcttgatct aattttcttt ttcgaccatg accgccttta
     8341 gaaaaaactc ctcggctttg ctcccttgat ttcattgctt taattttatt tttgaagtta
     8401 cttatatatt tgctgtcacc aaaatcaatc acagattgat tgttaactaa acatttaccg
     8461 ctacacaaat cagtaaactt tatggttaat tcagctcctt ctttgaaatc tagcttatga
     8521 taatcttttt caaaagaaat tgttaaattc aaaaataaat ttttcccctc aaagattaga
     8581 ttactctggt ttaacttgta ttgaccgtcc ataatgcgtt ctaaagttaa agcgctatct
     8641 ctggcttttc ttggttttct acctaaattt actttaaacc taaatccttt aggtaaagag
     8701 atataaaact tgtcagcttt gtgaatctta atatcccgac ctctgaacgg taacaatcca
     8761 tatttatttt tatttctata gattctaggt gttctttgcc cagtaacaat tccaacaaaa
     8821 tcattgctaa aatccttact agctttcatt ttagctgaat ctataaaatc cgaataatca
     8881 aagtccaatt tttttgctat atcacgtatt ctggtttgac tttttaaacc tatagcttgt
     8941 gtcaaaacgt ctctggcttc tttgtttttc gcaattcgta gtttagataa ttcccttttc
     9001 gttttatcta atttcttttg atcgatcact tcattactta gtaaattatc aattcgctct
     9061 tcaagactta gctctctttt aatatactca tgatccaatt cttttataga attttcgatc
     9121 aattcttcaa acatcagctg tttatatacc atattcagcg cttttacata ttctctacta
     9181 tttttccaaa aaaaaccaaa aaacgaatct ctttcctctt gtgatccatc aacatatact
     9241 tctatttttt tagtcatagt tattattgtc aattttagca cctcattatt atatatctgg
     9301 tatataataa tagtaacata tttcctagac tataatcaat gatcaattgt ctacttgtag
     9361 taattatatt aaaaacaaaa actaaaaata atatattatt ccttctgact acatatagtc
     9421 actttatcca aaaaataaga agtgctttcg cactccttat taaatttatt tactattaat
     9481 tgattcagct acaacttctt ttattcaact tcaaaatcag ttttttggat atttctatct
     9541 ctatttaatt ccatacttaa tagctcacta tcagttttta ctaaaagctc acctacacta
     9601 ttaatatatg ccaatttaca agaatttctt cttttatctt gacctactaa ttcatcttga
     9661 gatacccctg tagaaaaact aattgataga atccgttcct tgatttcatt tttatggttc
     9721 ctataaaaat catcgacttg cgcttctttt gaaaaccgat cactaataaa atcatcactt
     9781 gctgaatata ttctttcaag ggtttctttt ggattcatct cttcttgaga tatatccatc
     9841 ataatttgtt tgcttatagg agtttcagca tttttctctt gaatagcttt gggaacccaa
     9901 gcatattctt gcgtattatt ggctacaact gtatgatttt ctttaacata aatataagaa
     9961 aactcatctg aattatcata gatacttaca ttatcaaaat atttctctaa ctctgctaag
    10021 tttcttaaag aaaattcgta tctttttctt attaaatctt caggtacacc gtggccacct
    10081 ttagcaacac ggattttaac cctttcaatc gctctttctg gtgaatctag tcctatatag
    10141 attaaaaacg attcataacc taatttttta gcctttagaa gtctcatttt atcgggatat
    10201 ggattaccac caaaagtcgt ttcatggtga aaagattttc cttctttaaa atgacctttg
    10261 atctctctta gaacttcacg catacttttg tttatgtctg cttgtttttt ccaatttccg
    10321 ccaaaacttc tcaaaatttc atctgcatta attctatcag tatcaagttc ttttcttttt
    10381 aaattttgaa cttgataaat agtagactta ccggcgccgt taacgccccc aacaactaca
    10441 taatacggtt tttcattttc taaactcata ttttaaagtc tttctgatta attacttttt
    10501 tttgatctac tcgtgtcaca aaactagaaa gtgtaaggta gaaagtcctt tcttcctcac
    10561 tttctgcatt ttttaaggct tcatgaaaat catctacagt catattcaaa acatcaggat
    10621 acattttctt taattctttt acagcctctg tcatagctct cattcccttc ttattagtct
    10681 ttattaaagt actctgcttc agcttctagt ctatctttca agtcttttaa aacaaccgta
    10741 agtatatgat cttcaccatt tttttcaatt gattgcttta tcgcttcatc taacttatct
    10801 aaatagtctt ctataattcc taaattgttt acttcgataa atcggaagtc cggtaaattt
    10861 ttcatccgaa taccgccttt aggctgttcc tttacaaata aagatttgta atcattatat
    10921 tcattcccat taataaccaa ttctttcata ggctctaagt ggataaattt acgtgaagca
    10981 tagtctttca taataatctc tcccagtaga aaatcttctt ttttctatta tatcacgttc
    11041 tcaaagcgtt gagttattgg attgtttaaa ccatcaagac acatcatgct gcattttttc
    11101 atcactaccg gtgcttttcc gattgcttta actaccgcta cattagctaa caaactaatt
    11161 acttctccct cgataagctc tccgctccca ataacaacgc cctgtacttc catacctact
    11221 ttaaacgttt cttttttatt taccattatt ccgctctcct tccatgacta cttgtagtca
    11281 tttggatatt ttttagggga acggttttcc gttccctttc atcaaataga atccttcatc
    11341 atttcaacaa ataattaact ttcagtgtaa aaaaattctt cgtaattacc atcatgaaaa
    11401 atatcccatc tttgttcgtc attctcactg aagtaggtaa aataatatct gacagtatct
    11461 aacttattta attgctctct ggttttgtaa atacaatcca agtgatcttg cattcgatct
    11521 gaccaaaaca ttcttgtctg ctccatctat tatctctcct ttttattaaa tcaagggttt
    11581 gagtctttga ctacttgtag tctatggcta tatcatacag gaatgactac ttgtagtcaa
    11641 tggttttctt tagtttaatt aactgtctat tcgtagccaa ataatgtaaa aaaacgccta
    11701 gtttacacta gacgtttaaa caattcaata attcttcgtt ataccccatt aagggttgaa
    11761 ctgctttaat acttacttga tcgtcttctc taacggcttc tatgcttacc ccttttgtat
    11821 attggccatc tttcttatcc gtccaaattt gataataagt aagcccatat cgttcaataa
    11881 tctcaaagag aatagttatt aatttttttt gtttaaattt tggtaattca atacgcaatg
    11941 atttttcttg aatatcgaac tctggatttt tatttttaag ttgttcttca ataatcatat
    12001 agtctgtaga cgtcttatct aaatcaatta atgaatctga taagattgct attgtatcgt
    12061 tcataacaca tcaactactt tcttcttttt attccatttc atatacagga gtattaagag
    12121 ataatttatg tgtgaagttc acgatttttg atacatcttc taacttgaag cgtttaaaag
    12181 cttcttttcc tcttgttaaa ttatcattcc aatcaaatcc ctctcgcaaa ggtacatcga
    12241 cttttacttc actgattcct atcccttctc ttccaattcc tttttcttta aattcagttt
    12301 caataaaagt acgtccggct ttatcattat caacacaaag gactgtttct gaaggcaacg
    12361 atttaaactt attataatgc tctgtaaccc catgcaatac attacctacc tttaatccat
    12421 tcatgctaac aaacgtagca ttttcggctt tttctggctt ttgtgtaatg tacgacatca
    12481 aatctatttc actttcaaaa aaatacattt tatctggttg accaaaagtt acgttaaatc
    12541 ctgaatactc tttagagttt tcttgtattt ttttccatgt aacatccttt tgtttgcctt
    12601 catgaaaaaa ttttctagtt ccttgttccg tacacccaac gatttcttga tcttttttcc
    12661 ataagaacaa aacatttcct tttttatctt gtttaattag tcctttagat aaaaaatctt
    12721 ccactaaagc tgtatcgatt cttctctcat ttactaaata agactttgca acagataccg
    12781 gcacttcatt ttctttctga tattggtacg gagctttttt cacttttgta ttgaacgaat
    12841 gaacttcata atcactatct tcaagtttct gttttgcttc tttaaatgaa caatcgtagt
    12901 aggctttgat aaaatcaaaa atatctcccc ctaaatcctt tgagttccat ttaaaaaggt
    12961 ttttacgatc actgattaca caagaatcat gatcttctaa tctatagtat tcccttgaat
    13021 cttgctttaa acctatccca ttttgtttgc aaaaatcaac gatacttact ttttttgcct
    13081 ttttcaaatc caaacaaaaa ccccctttac tagaccatca ctagtaaaat tacgataagg
    13141 tacacttgta tttttcataa taatcctctg tacagaaaat aaagtttagc ccatccctaa
    13201 gagataactc ccaaaagcct atcttcttgc tctttttttc tctaattatc ttttttctaa
    13261 tactttttcc gtacttcaaa gccattttaa aagcgatttg tacatcttca caaattacaa
    13321 aatgactatt attaatttga cgatattcac cttgcgtttc taaaaaattt ctattttctt
    13381 gttctatcat tcatttatct actccatttc aaattttgtt gtattttcaa aattagtcaa
    13441 tttcaaaatc ttattctcca tttcttcaga aacaggtggt atttcaaacg tatcattttc
    13501 aatttcatta taaatttctc tcattgcacc gttgtaagcc atgatagacg tagctttttt
    13561 gtattccgta gaagaagtat ctactggaaa acctagcagt tcttctttat cgatctctaa
    13621 cttttcggat aaatttgtca atttcgtttc aatttcttct ttatgactat cataaaattc
    13681 attaatatca ctatctaatg ctaatctgga aactggttct tccatgtttt ctaagtattc
    13741 aatgcgctct tgccaagctt gtttgtcata ataattagtt gtttctagct cttgctcaaa
    13801 atcttttatc acaacttctc gaatgggaac ttccgaaacc ttatgcccaa caaatttatc
    13861 tgtcatgtcc tcactacttt ctaagctatt ccgttcctcg tttaaatcgt gaatagtttc
    13921 aatctttact ttatcaggct gtaacactac gataactaca tccttttgtc ttaactgttc
    13981 ttccgcaata atatgtgtat atttcccaac gtcataaaat gccttagcag tagcttcatg
    14041 atctccaatt gtatttccaa tgtcgttaat caaatctacg tcattttcat tctcactaag
    14101 agaagcaatc acttctcttt ttacaacttt ttctccgtaa taactaacat cattgtaatc
    14161 atttaaaata tcattacttt cttgtaattt atctattact ttagaaagct cttcatcagt
    14221 aatagtgcgt tcattcatag aaatcggctt ctctaattca acatttactt catatacgaa
    14281 accattattt ccgttactac cctgttcagc gtacattgta gccatttctt tattaggtgt
    14341 taagtacaca ccaaatccat tagctgttcc gtttaaacct ttatgatcta aactaaattc
    14401 atcaaattta ttcatacttc catgaaacag gcgataagtt ccgcctgttt cataattttg
    14461 ttcattactc ataatttttt cactccattt caaaagaacg ttctgctgtt tttacagtcg
    14521 gcttttccaa gttagaattt tctggtatct tgaaagttcc ttcatccatt tcatatttta
    14581 ctttagttgc aaccagttta taagctacca ctgaagccac ctctttatat gactcattgt
    14641 ctattgtatc tcttaaattt gttcctgaca cagattcaaa atctgtaatc gtattttcga
    14701 tttcttgttt atgagattcg taaaaatctt ccgcagtcat acaagagttt aattctttta
    14761 catcattaat atttgtttgg ttttcaattt cttcttgcca gcatttttca ttatagtaat
    14821 tgttagcatt ttcagataga ttataaaccg cttcatttat aacaactgat cttagcggtt
    14881 cgcttaattt taacgtatca ttttttagtt catgattctc gcaagctttc aaactttcta
    14941 caacaaactt ttcaacataa tcacaatttt caggtgatcg atctaatagt aaacgatcat
    15001 gtttattttc acttggattc tcaaaaacta caccataaac atcacataca tcaaattgtt
    15061 tcgtatctga ataatatgtc acatagtttt ctcctatttt agctgtaaca tcataattgc
    15121 tatgagtaag ctcaatagaa ttttctccat actttcctct ataataatcc accatcgctt
    15181 ctaaataact accgtggatt gttctatccg gatcgttgat tgtactatcg tagaaattat
    15241 gaaattctaa atctgatttt tgttgtatct gctctttatt ctcattagga acaaaggaat
    15301 tttttagtgc attaaatgct gtttgctgtt cttccaagac tttcttatca atcgtactta
    15361 taaagtcaat agacgtttct ctaacttctt gcattatctg tctaaagttt tttggatcaa
    15421 ttttttcatt atttttagtc caatttgcaa tataaggaat agtaaagtct gacgtatcaa
    15481 taccgtaatt gtgacaaaca ataaatgaag tcatttccgc ttgaaactct ttaatcggtg
    15541 ttgagtagga agaaccgtgt tcattcttat tgtgaagctt tttgtgagcc aattcgtgaa
    15601 tactggtagt aagattttgt gttctgctat ttcttgaatt taatacaatt tcttccctac
    15661 catcaacata ttgaatatat tcacctctag cgcttcccat ttcacctcta ccagactcgt
    15721 tcatatcttt tatagatata ttcatagatt cggccaaatg atttacacct ttttctaatt
    15781 gactaacact taattcacca tcaatattaa aattccatac cctgttagga aatatttttg
    15841 gcaaatcagc ttcagttgca ttagtttggg aaatatcaaa tacgtaaccc ttcttaaaat
    15901 gcttgatctt tttgcttctt aattctccat ttttgatttt ttctttttgc tcgtttgtcg
    15961 cttcttttaa cgtcttttcc tcaccatttt catcaataaa aaccgttacc ggagcatgcg
    16021 cataaatgcc tatccctttt tctttcgctt taacttgaaa tcctagtttt ttccaaccat
    16081 cataacttgc tacagcaatt gctccttcaa atccctcatc gatcaacgat aggtttaaag
    16141 gactgtaatt gtgtatttta gacatgaact tggcaaactc gatcatatct tcagggttag
    16201 actgatattg ctcgattttg ctgttggaag ccttaattaa atcgtctagc tctttttgtt
    16261 tttgctcttt cgttttcggc ttccagtttc cttttttata ggccatcttc tatctccttt
    16321 cttttttagg taagttaaat tacctaatta aattatacca gatatataaa aactcttcta
    16381 tctaatttca ctgacttaat gcaaataaaa aagagataag atagttatag taaccatctt
    16441 atcccttctt attttaccat ctactcaact atacttacgt ttttaccttt actatctaat
    16501 tgaactttgt tatctttaga agctacttta gtaagcaaaa gttgatccaa ttcatcatta
    16561 gaatatcctg aataaaaatt atctaaaact ttcgttgtgg caactggaat tgataacttc
    16621 aaatccatac tagtagattc ataagataat tctccttcag acgaaacttc accaaaacta
    16681 tttaaattcc aaattggact atctaaatat ttataagata atttaacggt ttccattgaa
    16741 ggtgatttgt tttcattatc cattttaatt gttgctgata ccattttcca ctccggctct
    16801 gaatattgaa caccatcgat ctcttctata tccgcctgta ttttaggtat actatcttga
    16861 aaatcagcaa ttttttggtc attttgaaac tcttgttttt cctcattttc taattctgtt
    16921 tttacagaat ctaaagtggc attatagcta tcaatatcgc tttttttctt atcattaaac
    16981 ttttgtttat cagcgttcaa tgcttgttct attatttctt catcactcat tttagaaact
    17041 ttttccaatg taagctgttc catatccgtt ttaaatactt ttgctttgcc atctttagtt
    17101 tggatcaccg cttttacata ttcattttta tcaataggcg ttttattttt actacttgtt
    17161 tcgactacca accaacaacg tgagtctttt tgttctgcac tatctatgaa tgagatatta
    17221 tgtgcttcat ttgacttagc accagcttct tctttgccac atgcagataa taatagcgca
    17281 aaactaaaaa tcataggtat tgttgcaata actcttaact ttttcattta taagcacctc
    17341 ttttttatcg gtctgatata taattttata ttatcatatt tttattccaa tacacagata
    17401 ctcttatata atgtcttttc atgtagttta tcaaatttct atttcaaacc gcttaataca
    17461 aatatcaaag cccaaaattt tctactggaa gcttttagag cctaaaatat attttttatt
    17521 tgatccaaaa cttcagggaa atacaaacct aatatcaaca cagcacctat aattaatagc
    17581 ggaatcattt tcttaataaa agagatcaca gcaaaaacta tcagtactgc aactaatact
    17641 aaagctgttt tgggattaga atataaaaac tctttgactg tagacgttac atttaagtat
    17701 agttcaatca atacgcgaaa atctccatta cttagtttat ccataaccgt tctaaaatcg
    17761 atcatatcat ttcctcactc tttccaattt tattgcttct tttattgaag aagcaacttg
    17821 ttcattacta tgcttatcta tatataatgg ggaaaagtaa tttttatcaa aaaagtaatt
    17881 tgcaaaagct acatcgtctt ctgtcttcca attataaaaa cagtaattcc cactaatcgg
    17941 tatttcaaaa agcccccaat tctctagctc catgttttct ttttcaaaca acactatgat
    18001 tttttttaca tcttctatag tttccgaatc actgttaagt agctcacgta aatatatatt
    18061 ttttgtatta ctgcacacta tatcgaagtt tttcataatt tctattctcc gtttctacta
    18121 attcattcct atttcaaatt cacgtgcttg tctcattttc tcttgctcta tcattcgttg
    18181 cgctacttca taatcatttt tcgcacgata catatttaaa tcatcattga ttactctatc
    18241 aatttttcta ataagctttt tatctttaaa tttttcagaa atcttcttga agtggacttc
    18301 atttttttca cgtgctaatt gtcgttcttc taatcttttt tggaaatcat cataactttg
    18361 tttagcatat ttcgttttaa attctggttt ttctctatca aacttaacgt ttaatttttt
    18421 tccgtctaat agatttttaa gttcattact tttcaaattt ttttcttcag attgctttaa
    18481 aagtttgatt tgtcctacta cagcattacc cattcgagca ttaaaatctt tttttctatt
    18541 taaataatat tcttgtggat tgtagcgttt accgtaggtt gtttgatata gctcatcaat
    18601 ttctttagtc atacgatcat attcttttct ttttggatta tcctttgtca tataatttgt
    18661 gacttcatca atcttttctt ttgtttcttc aggaagctca ttgtagccac gtgttgtact
    18721 aggtatttct ttttttaatt cctttaggta ctttactaat tctttgtcat atcttgaatt
    18781 attttctaca tataattgaa tcaaagaatc ttttacagac tgtttaagtt ctaaaggaac
    18841 ttcttttctc aacttagaaa ttttttcaaa ctcgttcgtt ctatctaata ttttgctacc
    18901 gaatttaaaa atcatatcgt ctaacgtaga ctgttttctc ttacccctag cttgtaaaac
    18961 accatcatat tccattattt cacgagtatt tttacgctcc atagctgtta catgaatatg
    19021 gatatgttca gtatttctgt gaatcgttgc aaaccaaaaa ggatcaacta gttcttcctt
    19081 ttcttgcatt tttcccatca tagactttgt tgcttcatat agaacatttt catttaattc
    19141 atttgtttct ggattataat atccttccct tactaaaaaa tcattatcga atgaaattac
    19201 atcttggaac atcaccgatc cattattttt tgcttctatg accgattctt ttatttttga
    19261 aatgtctttt ttagtgatat atctttttgt attgctaaat gctccgttta ctatttcatt
    19321 atctacattt tctaagatag cttttttacg tgtcatataa tcaatatatt tatccatttc
    19381 acgtaaatct ttaatcactg gttgatctgt ttcctgttct attttttcca aagctttttc
    19441 tattctagct aattcattta gttcatcttc tgaataatca tgatctagtt tatcttgcct
    19501 aatagcctct tgtctggaaa tataatctac aaatttttga agttctacca tgtgattatt
    19561 ctttttgctt tttccagttt caaattttgc tttaagaata atgctcgcac tttttctgtt
    19621 aaagatatgt tcagtcatat catcacccac cattttcaaa aattttttct acaggcggtt
    19681 ttgtacttaa tgctaagttg cattagaatt ttttagtgtt caatccgttg taaatcttaa
    19741 tgacggtgaa ctaaacattg ttccctctcc caaacgacta ctttcttgtt ctggttcagc
    19801 acgctcttct tctaattctt tactagctaa tccactatta attaatagtt tatagacaga
    19861 ctcttctata ctatttaatt gttcactata atctttatgt gtactgtctt cttttttatt
    19921 aaagttttca agttgctgtt tttgcaattc tattatcgtt tcaagttcgc taatttttaa
    19981 ttttgcttct tctaagtttt tagctaactc ttctttttct ggatcacgga aattttgatt
    20041 ggcttttgtt aacattggta aatttgaaac aacataacgg aacatattgg attttgtttg
    20101 aaatactgac ttgtttactt caaagcactt attgaatttt tcttttacat cttctgttgc
    20161 tgatactttg aaataagggt agttcattta tttaaatcac tcctttcgct aattaaaaaa
    20221 ttgaataagc gttcataaac tgaagttaac tcgctaatac tttctaccat ttgttcattt
    20281 gacaataaaa gtttttcaac tactaaatca aattctgact ttttatagtc aatcaaatcg
    20341 atctcgctat cttggcgaat gattgctaaa aaatagtcat tcctactaaa cttttttccg
    20401 gtacgttctt gaattttatt aactttttta tcaatctctt ttacatattg tggatcaatt
    20461 cctctgattg aaacatccat acacacacct ctttcaagct gtttttatct atctggtata
    20521 tattattata tcaaagtcca aaggaaaatt aaagatttaa agacaagacc aaggaattaa
    20581 tacaaagagc ataagggaaa actgatagca ccttcctaaa ggaaggtggc taagttggct
    20641 gtgccaactg gttttctttc aaaatagctt catatttttt gctatcacaa aaaaatccat
    20701 tttcgaccta ttttcggtca taatatagta cctacttttg gtcatagttt cgtccgtagt
    20761 tttggtcata gtaactaaag cttttacgcc caaaaaaaga gtggcaagtt ttaccttgcc
    20821 actctctttt ctattcacaa aattggttta aattcgcttt ttttagcact tcgtttatat
    20881 catagcaatc ataatattta cacaacatca ttcctaattt taacgcttgt tcctctttat
    20941 attttttaag ctctgcattt tttttgcgct taatcgcttt tctcttttct ttatattccg
    21001 cttcaattct cattaattct tcttcagctt tctcttgaat agtgacaata gaattacaca
    21061 ttctctctaa actcccttaa aataatttaa acacgatcac tcaaatcggc ttgcagactt
    21121 ttctagttgc tttatttttt cctcaaattg tttgatttta ttttcacttt cctctattga
    21181 agatttatta ttttcaatag tcgtctgcat agaactgatt tcattttttg tatctgtttg
    21241 ttcgtctagg gtttgaaatt ctaagttttg attagcttta tcaatatctt tttgcactac
    21301 tttatttgct aaattcattt tttcaattga ttttttctca tcttttatag aattttctat
    21361 agctttaatc tgatatttaa aattatcttt ttgcaaatca gaagtacttt caacttccaa
    21421 tttagcattt gatttaatat ctttgtctac tgcatagaat tttatttcct tttcttttaa
    21481 gtcatttgaa ggttctaatt caggataaat atatctaggg ttaactgtca cagaaattac
    21541 accaaaatct ttaggcagat tttgataata aattaccgtg tattcatctg acaattttac
    21601 aacttttgtc ttaacatctt cattattttt gttcgttata aaatttaatt tatagtcact
    21661 tttaatatta cttagtgatt tagtacctgt atcatcttga taccgcaata ctaatttaaa
    21721 cagtccggct tccctattta tttccatatc atcaacatac atattcaaag aagacgttac
    21781 attttgggct gaactcattt ttacagtttc aaaatcataa cttttcccca tcacataagg
    21841 agaaaaaacg attatcattc ctattgctaa aaatccaagt atagcaccta aatataattt
    21901 tactcctttc gtttttttat atactttgtc aatttgtgcc ttattttttt tatttggttc
    21961 cttcttatta tgaatggaat atttcttatt catatacttc acctcactat tttaagtact
    22021 tgatttcata attatctacc aacacttggc cgtctttatt ggttacttca aaaagcatat
    22081 agactgtatt ttctgtatct ccactagaag attctgtttt ataatttact tcagcaaaat
    22141 aattaatagt attggcgtta acataattcg tatataattt tgaatcttta tatttacttt
    22201 tgaatgttgg atcacttgaa tatgcttctc ctttttgagt agtagattta ctttctaaag
    22261 tctttttact cgttgtaatc atgtcgtccg gcacgataga atcagctaca gaaggagtag
    22321 ctattttctc taaatcagct ttcttttctt ctactttttc aatatcatat accggatata
    22381 gctcaacaaa tttcttagca acttcagcaa tttgtttatt taattcactg ccatcatcat
    22441 tagttaagtt cgcttctaca tttgcattta gatcactaat atttttactt aacttgctat
    22501 tttgttcttc tagttgtgta acttcttgat ccaatttttt attttttgct tgaatgttac
    22561 ttgtatgaag ttgagataac aatccaccta aaactaaaat aataattaaa acaccacaaa
    22621 tatatttcca tttattatca ttttttctct taatcatcta ttttcttcct cctatttttt
    22681 gactctacga atctctggac tatgtgattg ccaatatgaa tcttgccaat tgtgataacc
    22741 aattccgctt gaattactgt catacatagt tgtttcattg acataaatac ctacgtgaga
    22801 aatgaaatca ggcgctccat atgttcctct aaagaaaatc agatcaccag cttttgcttc
    22861 tttaattgat actgcttcag ttgcttcata ctgcgttact gtataactat ctattgtaat
    22921 tccggcgcta aattgataaa gataagctgt taatccgcta caatcaaaac ctgtcgttgg
    22981 atttttaccg ccccaaacat aaggccaacc ttgatatttt tccatttctg ttttcatttt
    23041 tccccaaaat tctgaaggat tagccggatt accactaccc ccaccgatat actgtttaac
    23101 taactctgca tagtaaaaat taccaccatt taaatacaaa tatgtttttc catatttaat
    23161 tgatacttca tttacatatg aataagtctg tcctgtagta tttcctaaac ttggagcaac
    23221 aacattttta gagtaggctt cagccgtttc gatagtatgt gtttttttgt tttttcctaa
    23281 gtagtgcatg taagctattc cgtaattgta agcttgaact attccatcat agttactttc
    23341 catgccataa cttttagcaa tgttatacac attactcaaa tgttttagcc cttgatcgat
    23401 actttcttca tattgaagtg tgtttacagg caatccggct gattcgctac tttgcattaa
    23461 atccggcaat ttacctccgg attcacattg cataattgct aaagcataag gtactaattc
    23521 tttaattccg tatttttcag catatttttc taccgttttt tgccattttt gaacttcagg
    23581 tgacaacggt ttgttgccac caatgacttc tgcacctcct gtattagttg cagtttgtgt
    23641 tccttgtaat gtagctaaag aacctataac taaaaggcca accaatgtta tcagtcctat
    23701 aactacccct atttttattt ttaaagcaag acctattttc tttgatactt gcttcatccc
    23761 ttgacgagcg agtgttgaag caacattatt atttttgttc tgctccatta aattcacctc
    23821 cgatttctat tattgaccac catcgaatcg ttcaatttgc tctggatatg gatcaaaatt
    23881 aatcatatag gtgtcttcac tggaagtttg aactacagct tgtcctactt ctaattctgg
    23941 taaaagcata atttctgtat cagtaattgt atttcctaaa acttttttta tgtcttccaa
    24001 caatgcagaa tctaaattaa agaagcattt aaaagtacat agattaaaaa tgtttctaat
    24061 cacatctgct acttctgatc cagaatcttt agggataatt tcttcaggcg tttgagttgc
    24121 aaaataaaca cctgcacgca tttttcgcat ttctcgctca aaacttagta catattcaca
    24181 agcatacttg ttatttgaat tgata
//
LOCUS       DACEKQ010000039        23819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEKQ010000039 DACEKQ010000000
VERSION     DACEKQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.041"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW671_001411"
     CDS             complement(45..779)
                     /locus_tag="IW671_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0898399.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IW671_001412"
     CDS             1106..1516
                     /locus_tag="IW671_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0898400.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IW671_001413"
     CDS             1509..2210
                     /locus_tag="IW671_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0898401.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IW671_001414"
     CDS             2210..3796
                     /locus_tag="IW671_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0898402.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IW671_001415"
     CDS             3815..4036
                     /locus_tag="IW671_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0898403.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IW671_001416"
     CDS             4033..4794
                     /locus_tag="IW671_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0898404.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IW671_001417"
     CDS             5174..5683
                     /locus_tag="IW671_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0898405.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IW671_001418"
     CDS             complement(5758..6297)
                     /locus_tag="IW671_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0898406.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IW671_001419"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IW671_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0898407.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IW671_001420"
     CDS             7364..8161
                     /locus_tag="IW671_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0898408.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IW671_001421"
     CDS             8189..8824
                     /locus_tag="IW671_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0898409.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IW671_001422"
     CDS             8844..9617
                     /locus_tag="IW671_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0898410.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IW671_001423"
     CDS             10025..10822
                     /locus_tag="IW671_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0898411.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IW671_001424"
     CDS             10800..11213
                     /locus_tag="IW671_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898412.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IW671_001425"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IW671_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0898413.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IW671_001426"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IW671_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0898414.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IW671_001427"
     CDS             15990..16550
                     /locus_tag="IW671_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0898415.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IW671_001428"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IW671_001428"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0898416.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IW671_001429"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IW671_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0898417.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IW671_001430"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IW671_001430"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0898418.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IW671_001431"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IW671_001431"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0898419.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IW671_001432"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IW671_001432"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0898420.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IW671_001433"
     CDS             21897..22475
                     /locus_tag="IW671_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0898421.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IW671_001434"
     CDS             22527..23009
                     /locus_tag="IW671_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0898422.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRGAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IW671_001435"
     CDS             23126..23500
                     /locus_tag="IW671_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898423.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7773 a         4286 c         5116 g         6644 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaactac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga ggagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagtt
//
LOCUS       DACEKQ010000040        23656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEKQ010000040 DACEKQ010000000
VERSION     DACEKQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.043"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            257..532
                     /locus_tag="IW671_001436"
     CDS             257..532
                     /locus_tag="IW671_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898424.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IW671_001437"
     CDS             545..2623
                     /locus_tag="IW671_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0898425.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IW671_001438"
     CDS             2783..4669
                     /locus_tag="IW671_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0898426.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IW671_001439"
     CDS             4681..5628
                     /locus_tag="IW671_001439"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0898427.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IW671_001440"
     CDS             5648..6175
                     /locus_tag="IW671_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0898428.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IW671_001441"
     CDS             complement(6238..6891)
                     /locus_tag="IW671_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0898429.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IW671_001442"
     CDS             7023..7865
                     /locus_tag="IW671_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0898430.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IW671_001443"
     CDS             8023..8919
                     /locus_tag="IW671_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0898431.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IW671_001444"
     CDS             8922..9635
                     /locus_tag="IW671_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0898432.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IW671_001445"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IW671_001445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0898433.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IW671_001446"
     CDS             10170..10397
                     /locus_tag="IW671_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0898434.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IW671_001447"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IW671_001447"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0898435.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IW671_001448"
     CDS             complement(11183..11725)
                     /locus_tag="IW671_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0898436.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="IW671_001449"
     CDS             complement(11962..12360)
                     /locus_tag="IW671_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0898437.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="IW671_001450"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="IW671_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0898438.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="IW671_001451"
     CDS             13473..14240
                     /locus_tag="IW671_001451"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0898439.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="IW671_001452"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="IW671_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0898440.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="IW671_001453"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="IW671_001453"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0898441.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="IW671_001454"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="IW671_001454"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0898442.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="IW671_001455"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="IW671_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0898443.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="IW671_001456"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="IW671_001456"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0898444.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="IW671_001457"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="IW671_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0898445.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="IW671_001458"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="IW671_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0898446.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="IW671_001459"
     CDS             22691..23566
                     /locus_tag="IW671_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0898447.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6402 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcga
//
LOCUS       DACEKQ010000041        23578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEKQ010000041 DACEKQ010000000
VERSION     DACEKQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.044"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            45..677
                     /locus_tag="IW671_001460"
     CDS             45..677
                     /locus_tag="IW671_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0898448.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(679..1584)
                     /locus_tag="IW671_001461"
     CDS             complement(679..1584)
                     /locus_tag="IW671_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0898449.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1669..2322)
                     /locus_tag="IW671_001462"
     CDS             complement(1669..2322)
                     /locus_tag="IW671_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0898450.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            2644..3588
                     /gene="mvk"
                     /locus_tag="IW671_001463"
     CDS             2644..3588
                     /gene="mvk"
                     /locus_tag="IW671_001463"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0898451.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3581..4558
                     /gene="mvaD"
                     /locus_tag="IW671_001464"
     CDS             3581..4558
                     /gene="mvaD"
                     /locus_tag="IW671_001464"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0898452.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4571..5656
                     /locus_tag="IW671_001465"
     CDS             4571..5656
                     /locus_tag="IW671_001465"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0898453.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5653..6717
                     /locus_tag="IW671_001466"
     CDS             5653..6717
                     /locus_tag="IW671_001466"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0898454.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6966..7769)
                     /locus_tag="IW671_001467"
     CDS             complement(6966..7769)
                     /locus_tag="IW671_001467"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0898455.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7789..8631)
                     /gene="kduI"
                     /locus_tag="IW671_001468"
     CDS             complement(7789..8631)
                     /gene="kduI"
                     /locus_tag="IW671_001468"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0898456.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8647..9654)
                     /locus_tag="IW671_001469"
     CDS             complement(8647..9654)
                     /locus_tag="IW671_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0898457.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9661..10305)
                     /locus_tag="IW671_001470"
     CDS             complement(9661..10305)
                     /locus_tag="IW671_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0898458.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10405..11163)
                     /locus_tag="IW671_001471"
     CDS             complement(10405..11163)
                     /locus_tag="IW671_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0898459.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11290..12441)
                     /gene="fucO"
                     /locus_tag="IW671_001472"
     CDS             complement(11290..12441)
                     /gene="fucO"
                     /locus_tag="IW671_001472"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0898460.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12523..12834)
                     /gene="rhaM"
                     /locus_tag="IW671_001473"
     CDS             complement(12523..12834)
                     /gene="rhaM"
                     /locus_tag="IW671_001473"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0898461.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12836..13681)
                     /gene="rhaD"
                     /locus_tag="IW671_001474"
     CDS             complement(12836..13681)
                     /gene="rhaD"
                     /locus_tag="IW671_001474"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0898462.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13695..14951)
                     /gene="rhaA"
                     /locus_tag="IW671_001475"
     CDS             complement(13695..14951)
                     /gene="rhaA"
                     /locus_tag="IW671_001475"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0898463.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14948..16408)
                     /gene="rhaB"
                     /locus_tag="IW671_001476"
     CDS             complement(14948..16408)
                     /gene="rhaB"
                     /locus_tag="IW671_001476"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0898464.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16561..17430
                     /locus_tag="IW671_001477"
     CDS             16561..17430
                     /locus_tag="IW671_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0898465.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17556..18455)
                     /locus_tag="IW671_001478"
     CDS             complement(17556..18455)
                     /locus_tag="IW671_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0898466.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18620..19444)
                     /locus_tag="IW671_001479"
     CDS             complement(18620..19444)
                     /locus_tag="IW671_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0898467.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19455..20750)
                     /locus_tag="IW671_001480"
     CDS             complement(19455..20750)
                     /locus_tag="IW671_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0898468.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20766..21245)
                     /locus_tag="IW671_001481"
     CDS             complement(20766..21245)
                     /locus_tag="IW671_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0898469.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21251..22279)
                     /locus_tag="IW671_001482"
     CDS             complement(21251..22279)
                     /locus_tag="IW671_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0898470.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22296..23402)
                     /locus_tag="IW671_001483"
     CDS             complement(22296..23402)
                     /locus_tag="IW671_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0898471.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5200 c         4239 g         7360 t
ORIGIN      
        1 ttcccgcttc tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac
       61 ctattccacc ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga
      121 tcttctctgg tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg
      181 tattaagaga gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg
      241 aatgcagtaa tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca
      301 gtttccacca agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt
      361 atcttcgaga ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa
      421 acttgaaagc tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca
      481 cttggaaaaa cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt
      541 ctctgaaggg attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt
      601 ctttggcggg actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt
      661 tgcagatcaa agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga
      721 taagaccaaa ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa
      781 ctggagattc attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca
      841 gaccgtcaaa aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc
      901 agagagctgg tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc
      961 gatcagcgca tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa
     1021 tgccttgatt tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa
     1081 accgatctct aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt
     1141 cagattaagc cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga
     1201 aatcaaaaac aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa
     1261 aagcgatcga attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc
     1321 gtcttctttt aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg
     1381 gtgcggaaca tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc
     1441 ccgttcattt ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt
     1501 taagttttta acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg
     1561 attaaaatag tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga
     1621 aaaaagtcgc tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac
     1681 tttggtttgg cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc
     1741 ttgaggatct tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt
     1801 gtattcttca taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact
     1861 tggatcaaag gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt
     1921 atcaaaagat acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg
     1981 aagttcttcc atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc
     2041 tcttcctaga tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa
     2101 tacattattg tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata
     2161 aatttgcagc aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt
     2221 atcatcaatc gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa
     2281 acttgatttt ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct
     2341 cacttcatca ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta
     2401 gtagtgtcaa ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga
     2461 aaaaagtgtt ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta
     2521 aaataaagga ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc
     2581 aactcgtttt gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag
     2641 attatggcaa actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac
     2701 gcggttgttt atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca
     2761 ttccttgaag agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat
     2821 ttagccgaag ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa
     2881 cagcatgaca tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt
     2941 ggaatgggat caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta
     3001 gcatttcctt tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc
     3061 cacggtaatc ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt
     3121 acaaaagggc atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct
     3181 gatacaggaa tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa
     3241 acccagcctc atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca
     3301 aaacaagcaa tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa
     3361 tcacttctgg aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca
     3421 aaagataccg gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt
     3481 gcattagcac aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt
     3541 gctgctgaaa cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg
     3601 cgcatatacc aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct
     3661 tccaatgaac aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat
     3721 cttttctgat agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa
     3781 agcaacaaaa aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact
     3841 aaaagcttca gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc
     3901 cagcgggcta gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga
     3961 tctctctctt tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg
     4021 tggtttcgtc gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc
     4081 ttccgattct ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa
     4141 agaagtgtcc agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg
     4201 ctggttagat tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga
     4261 tttccaactt ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt
     4321 agctgcccag cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt
     4381 gcgacaattg agaaagcaag gcattccatg ctactttacg atggatgctg gccctaatgt
     4441 caaagtttta gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt
     4501 tagcaaagaa caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag
     4561 ggagattttt atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc
     4621 agttgttgaa acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac
     4681 tgtagaatct gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt
     4741 acgttggaca agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta
     4801 tatccttgct gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc
     4861 cttttatgat ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt
     4921 gggttctagc ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt
     4981 gaatttatct cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga
     5041 taatggttcc tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac
     5101 cttcgaccat ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc
     5161 cctggattgg ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt
     5221 gattggttgg acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc
     5281 gagagaagat aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa
     5341 cgaaatgatc aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa
     5401 aaaccgacaa ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc
     5461 tttgaacaaa ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc
     5521 aggtgggggc gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat
     5581 gagtgcatgg gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag
     5641 aaaggaaaac cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat
     5701 aaacaaaaaa acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca
     5761 gtttctgatg tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat
     5821 atcaatgcga tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc
     5881 gtcgcaagag aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc
     5941 ccttctttag ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt
     6001 gccaacatcg gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac
     6061 gcagatgcct tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac
     6121 cgtgatttta ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc
     6181 ctcatcgtaa aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct
     6241 ttaggagttc atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa
     6301 aacgcccgtc gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc
     6361 tcttcattgc ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt
     6421 atccgcaatg cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact
     6481 tcaggaaccg tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg
     6541 aagcaatggc aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg
     6601 gccctacacc aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga
     6661 gggatcgact tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga
     6721 acacttttgt ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg
     6781 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct
     6841 tggagttaaa tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt
     6901 ccttcggtat tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag
     6961 ctcttttaag gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg
     7021 ccgttaacga aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt
     7081 ggctcgcccc agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt
     7141 tcaccgtttt cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt
     7201 ccaattccat tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc
     7261 atttttaatc cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc
     7321 atcatggagc agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact
     7381 gcttttgaaa cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc
     7441 gcatccattt cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca
     7501 actgaaccta catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc
     7561 acatcgcaga cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca
     7621 tcaactgatt cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag
     7681 cgtgcaatct caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct
     7741 aaacgaaaca tatccatact aaattccatc attttctctc ccttatgctt attttaattg
     7801 atccatttgc accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat
     7861 gaatgagtaa ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc
     7921 ttgttcattg tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac
     7981 acgtgtatct tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt
     8041 atgacaaggc atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa
     8101 ttgacagctt tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt
     8161 caggccttca ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata
     8221 tttatgatga gctggaacag aactaatata gaatttagct gggttttcag catcttgaga
     8281 agaaaataca acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc
     8341 ttctttttgc ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc
     8401 acgacgctct aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt
     8461 ttcagtaggt gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat
     8521 tttccctggt tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata
     8581 atgacggata tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta
     8641 ctccttctat cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg
     8701 actaaattgg ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa
     8761 atcaatgatt tcttgcggag acatattgga taaaatccca tggagcacgc cacctgcaaa
     8821 agcatctccg ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac
     8881 atattcatgg ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt
     8941 acgcttggtc gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata
     9001 ataaattaga ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc
     9061 atccattttt ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct
     9121 ggcttcttgc tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc
     9181 agcttttttt gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc
     9241 tggggtgatt cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca
     9301 ttccaaatga tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc
     9361 gcgttcgcca ctaccagttt ccatataata agtacctagt cttgatccac cagataatac
     9421 ctgtttcgta tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt
     9481 atcaggtact tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac
     9541 gtttgcttcc cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc
     9601 ctgctctgtg gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag
     9661 ctacctcttg atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc
     9721 aaaatctcct gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc
     9781 agcatcgaac cactctttca tattgtccag actcacaccg ccagttggca tgatattgac
     9841 ttgaggcatc ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa
     9901 tagtttgacg atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca
     9961 tcctggtaag taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga
    10021 agggctaaca atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa
    10081 tactgtgcct gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt
    10141 gattacttga tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt
    10201 gatgattgcg tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat
    10261 cactccggcg tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt
    10321 cctatttaga aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa
    10381 gaaaaattgc aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt
    10441 ttgcttgagg gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat
    10501 acttacacta aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa
    10561 aatatcacgt tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgtttggat
    10621 ttcctttttc aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg
    10681 cttttcaaga taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat
    10741 cgcagaattg tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg
    10801 ttttggatct agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat
    10861 agtctcatcg atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc
    10921 gatttgttca atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt
    10981 ctctgaattt ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt
    11041 agaagaggtc attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc
    11101 taaatagtct agtattttag aggctttgat caatacagtt ccataaggct ttttattttc
    11161 catataatat tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa
    11221 aagagatatc cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc
    11281 gcttcgtttt tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct
    11341 tggatttcct ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc
    11401 ttcttttaca cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc
    11461 gatctcacca caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat
    11521 ccctagaaca agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat
    11581 gacagttggc aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa
    11641 cgggtgagcc atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat
    11701 atactgtcct aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa
    11761 agaatggccg atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc
    11821 ttttgtgata aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa
    11881 ttttttcggc attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg
    11941 aggatcaaca caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt
    12001 tacttctgct gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt
    12061 cgtatcagca acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat
    12121 cccaatggct tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc
    12181 ctctgcctct ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac
    12241 gatcccgtcg tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt
    12301 tgctataccg tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg
    12361 ctgctgaaat tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt
    12421 taaaatcatt cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa
    12481 aacgaaagaa ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg
    12541 catcaattcc caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta
    12601 cttccaccat ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc
    12661 gatcactaag taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat
    12721 gaccccgtgt tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt
    12781 gtattcttct tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga
    12841 cacctgcttt cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat
    12901 ctgctaaatg ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga
    12961 cgtacgtata gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca
    13021 tgtcttttcc tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca
    13081 ttttttcagc tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg
    13141 aaattccttc tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa
    13201 aggaacgttc gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat
    13261 gcataacgat ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac
    13321 ttggcaactc gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt
    13381 tgccatcttc agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt
    13441 taaaatatct tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa
    13501 aaatcatcga gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt
    13561 cttttagtaa ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat
    13621 tcgacgtcgt ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca
    13681 atatagccac actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct
    13741 tccagccaat ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg
    13801 gcatacggga aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc
    13861 agctccattt ctttcaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt
    13921 gtatttctcg taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc
    13981 agaccgatat ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc
    14041 aattcatcat ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc
    14101 aacatgattc ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt
    14161 ggatgaaaat gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata
    14221 taaaattcat gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct
    14281 actgcatctt gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc
    14341 attaaacgtt cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc
    14401 caaaagttat tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg
    14461 cttcttttcc cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta
    14521 aagccgtctt tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct
    14581 tctttttttg cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg
    14641 acagattctt ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc
    14701 aacgaataag ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga
    14761 aaattccctg tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg
    14821 acatcatctc cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca
    14881 gcatctgtat ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca
    14941 atcgtgttca tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct
    15001 tattttcatc tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt
    15061 gttctatatt cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg
    15121 tggcttcact aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca
    15181 gtagacgatt attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta
    15241 agtgttctac tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac
    15301 gagcgatagc tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa
    15361 tctcttctat catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct
    15421 gcagcggttt ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt
    15481 gcattcgggc aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg
    15541 tgttatgggc accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg
    15601 cgatctttgt ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt
    15661 ctccttgacc tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa
    15721 aggtcacatc tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg
    15781 ttcccggctc tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt
    15841 ctatttccca ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct
    15901 ccgtcaccat gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat
    15961 gtgcttttgc caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct
    16021 ggatctgaat ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt
    16081 ctactgctcc attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt
    16141 gttcatcaat cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa
    16201 tccctgtctg ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct
    16261 tccagcgatc gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca
    16321 agatcaaaga accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga
    16381 tatcgatggc aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag
    16441 cactttatta ataaagcgtt atcatttatt aacaatgcta tcataataca gaatcttata
    16501 ttttgcttca cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac
    16561 atggattact gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg
    16621 acgcctacac aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa
    16681 gcaccaatcc cttactatac tgaacgagca catttacctt cttacttgat aaagtttact
    16741 ttgtctggca aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac
    16801 ttatttttta ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg
    16861 gaaatggatt ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca
    16921 aaagacggcc ataacacctt ccattcaaca ggggatccat cagaaaacaa gatccaccat
    16981 ttgatcacag aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc
    17041 tctgtagcta tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc
    17101 gaagaagagg atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc
    17161 aaagaaacga ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg
    17221 agcaaagatt tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa
    17281 ctttcttacg ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa
    17341 gaaataggga ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta
    17401 tcgccgagag actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa
    17461 atattttgaa atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa
    17521 cagcagctac tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca
    17581 taatgaacca actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac
    17641 tctctcttga atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca
    17701 gcactagagc tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt
    17761 acatactgat gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg
    17821 ctgacaaaaa actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag
    17881 atatattgta acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca
    17941 ctctcttctt tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca
    18001 tattttatgt cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt
    18061 tgcttcaata cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga
    18121 aagcaagccg atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca
    18181 aagatataga cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg
    18241 gaatggatga gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa
    18301 gtcgcttctc cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt
    18361 ttattttggc tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta
    18421 ttgacttgtt caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt
    18481 atgcacaatt ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct
    18541 tataaaaaag aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt
    18601 aaccaacgtc ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt
    18661 ttgtttcttc aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt
    18721 ttgtgtactc gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac
    18781 gaaaatcttt caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta
    18841 ccaattggac ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga
    18901 ttcctagata gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca
    18961 ccggatgatt caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta
    19021 ccaagcgacg gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg
    19081 tatattggat ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg
    19141 aagaagcata gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat
    19201 ctggatgaat catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta
    19261 aaactcgttt gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt
    19321 caggaaacga aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt
    19381 ctgcaagatc ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca
    19441 acataactat tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa
    19501 gtaatcaaaa acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt
    19561 gcctctgctt ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc
    19621 gtgatggagc ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc
    19681 cctacacttg tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca
    19741 tccataaaca tacccaccag taataacaca agattgacta ataacagaat aataaattta
    19801 ttatccgtta cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg
    19861 gcgaaagaca tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact
    19921 gattgaaaca acatattagg tatatctttt agttttaccg ttttatagta aaacatagca
    19981 atcaacaggg agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc
    20041 aagataccac cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt
    20101 tttccagcat gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg
    20161 atgaatgcta ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata
    20221 aacaaagcag agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt
    20281 ggaggaatga ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg
    20341 tcatatcctt cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca
    20401 atggccgagc tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa
    20461 gcccccggca cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct
    20521 ccattattca tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta
    20581 ttgatacttc ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact
    20641 aacaataaag tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca
    20701 agcaacacaa caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc
    20761 catttttata ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc
    20821 aggctataga taatgatgag tacccccgat acaggtactg acagataaac atatcccata
    20881 ggaatactta aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca
    20941 tacaccatga tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt
    21001 ttactttttt tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt
    21061 cccacaacat acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct
    21121 tccgtgaccg tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca
    21181 gcgaccatcc caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt
    21241 ttcatcacag ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat
    21301 cgtttttgaa tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat
    21361 cattgaattg gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg
    21421 caaaagcttc aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct
    21481 gctcatcagt tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg
    21541 tatgctcatc ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa
    21601 attcattgtt ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac
    21661 ttcccatagg tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt
    21721 gcatcacacg gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca
    21781 tataaaagct gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg
    21841 tcgattggta gattggctcc attatttctt cattatccat caccttaaag aaatgttctt
    21901 catcattgaa tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg
    21961 ctgaggcact tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac
    22021 gttctccacc cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt
    22081 cttttactaa acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat
    22141 aagcaaagcg caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag
    22201 aattatttcc gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca
    22261 gcagtgatgt ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct
    22321 gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga
    22381 taaaaatcta aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt
    22441 tcatcgattt gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt
    22501 ggtaaataac gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa
    22561 gattctacgt aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga
    22621 taagttttta atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca
    22681 tcttctttcg ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag
    22741 tttccacgag cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga
    22801 taccacaatc ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga
    22861 gcttcttcta aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact
    22921 gtcatcatca atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc
    22981 tgtaacccgt cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct
    23041 aaataaggca aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc
    23101 ggtgccattg tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat
    23161 tcattgacca cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca
    23221 tatagaccga caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt
    23281 ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg
    23341 agcaggtcaa ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc
    23401 atctttacgc cactttttta aacgctttca ttttatatcc ttaatatagc agtaataccg
    23461 tccactataa atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata
    23521 acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caacgttt
//
LOCUS       DACEKQ010000042        23574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEKQ010000042 DACEKQ010000000
VERSION     DACEKQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.045"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW671_001484"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW671_001484"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0898472.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW671_001485"
     CDS             complement(1172..1867)
                     /locus_tag="IW671_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0898473.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW671_001486"
     CDS             complement(1880..2596)
                     /locus_tag="IW671_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0898474.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IW671_001487"
     CDS             2926..3531
                     /locus_tag="IW671_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0898475.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IW671_001488"
     CDS             3856..4179
                     /locus_tag="IW671_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0898476.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IW671_001489"
     CDS             4361..5731
                     /locus_tag="IW671_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0898477.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IW671_001490"
     CDS             complement(5861..7162)
                     /locus_tag="IW671_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0898478.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IW671_001491"
     CDS             complement(7392..9383)
                     /locus_tag="IW671_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0898479.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IW671_001492"
     CDS             complement(9527..9793)
                     /locus_tag="IW671_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0898480.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IW671_001493"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IW671_001493"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0898481.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IW671_001494"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IW671_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0898482.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IW671_001495"
     CDS             13137..13700
                     /locus_tag="IW671_001495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0898483.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IW671_001496"
     CDS             complement(13953..14474)
                     /locus_tag="IW671_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0898484.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IW671_001497"
     CDS             complement(14477..15319)
                     /locus_tag="IW671_001497"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0898485.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IW671_001498"
     CDS             complement(15312..16037)
                     /locus_tag="IW671_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0898486.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IW671_001499"
     CDS             16213..16509
                     /locus_tag="IW671_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898487.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IW671_001500"
     CDS             17077..18669
                     /locus_tag="IW671_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0898488.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IW671_001501"
     CDS             18682..19422
                     /locus_tag="IW671_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898489.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IW671_001502"
     CDS             19590..20939
                     /locus_tag="IW671_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0898490.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IW671_001503"
     CDS             complement(21067..21657)
                     /locus_tag="IW671_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0898491.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IW671_001504"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IW671_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0898492.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6753 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa aaaa
//
LOCUS       DACEKQ010000043        23489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEKQ010000043 DACEKQ010000000
VERSION     DACEKQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.046"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW671_001505"
     CDS             107..970
                     /locus_tag="IW671_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0898493.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW671_001506"
     CDS             complement(1099..2721)
                     /locus_tag="IW671_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898494.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW671_001507"
     CDS             2970..3491
                     /locus_tag="IW671_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898495.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW671_001508"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW671_001508"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0898496.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW671_001509"
     CDS             5572..6390
                     /locus_tag="IW671_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0898497.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW671_001510"
     CDS             complement(6493..6690)
                     /locus_tag="IW671_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898498.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW671_001511"
     CDS             6859..7437
                     /locus_tag="IW671_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0898499.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW671_001512"
     CDS             complement(8482..8985)
                     /locus_tag="IW671_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0898500.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IW671_001513"
     CDS             9344..10369
                     /locus_tag="IW671_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0898501.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10716..11594
                     /locus_tag="IW671_001514"
     CDS             10716..11594
                     /locus_tag="IW671_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898502.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12070..12186
                     /locus_tag="IW671_001515"
     CDS             12070..12186
                     /locus_tag="IW671_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0898503.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13114..13452
                     /locus_tag="IW671_001516"
     CDS             13114..13452
                     /locus_tag="IW671_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898504.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13546..13776
                     /locus_tag="IW671_001517"
     CDS             13546..13776
                     /locus_tag="IW671_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898505.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14176..14547
                     /locus_tag="IW671_001518"
     CDS             14176..14547
                     /locus_tag="IW671_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0898506.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14821..15513)
                     /locus_tag="IW671_001519"
     CDS             complement(14821..15513)
                     /locus_tag="IW671_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0898507.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15516..16301)
                     /locus_tag="IW671_001520"
     CDS             complement(15516..16301)
                     /locus_tag="IW671_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0898508.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16538..17938
                     /gene="lacG"
                     /locus_tag="IW671_001521"
     CDS             16538..17938
                     /gene="lacG"
                     /locus_tag="IW671_001521"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0898509.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17963..18280
                     /locus_tag="IW671_001522"
     CDS             17963..18280
                     /locus_tag="IW671_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0898510.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18293..19966
                     /locus_tag="IW671_001523"
     CDS             18293..19966
                     /locus_tag="IW671_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898511.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19980..21611
                     /locus_tag="IW671_001524"
     CDS             19980..21611
                     /locus_tag="IW671_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0898512.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22483..>23489
                     /locus_tag="IW671_001525"
     CDS             22483..>23489
                     /locus_tag="IW671_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0898513.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     S"
BASE COUNT         7328 a         3961 c         4758 g         7442 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa attttgttta
    10561 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
    10621 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
    10681 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
    10741 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
    10801 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
    10861 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
    10921 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
    10981 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
    11041 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
    11101 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
    11161 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
    11221 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
    11281 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
    11341 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
    11401 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
    11461 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
    11521 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
    11581 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
    11641 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    11701 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    11761 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
    11821 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
    11881 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
    11941 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
    12001 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
    12061 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
    12121 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
    12181 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
    12241 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
    12301 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
    12361 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
    12421 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
    12481 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
    12541 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
    12601 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
    12661 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
    12721 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
    12781 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
    12841 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
    12901 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
    12961 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
    13021 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
    13081 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
    13141 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
    13201 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
    13261 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
    13321 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
    13381 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
    13441 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
    13501 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
    13561 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
    13621 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
    13681 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
    13741 gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt
    13801 tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat
    13861 gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag taagtagcta
    13921 cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag
    13981 cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac
    14041 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
    14101 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
    14161 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
    14221 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
    14281 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
    14341 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
    14401 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
    14461 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
    14521 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
    14581 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
    14641 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
    14701 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
    14761 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
    14821 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
    14881 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
    14941 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
    15001 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
    15061 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
    15121 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
    15181 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
    15241 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
    15301 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
    15361 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
    15421 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
    15481 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
    15541 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
    15601 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
    15661 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
    15721 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
    15781 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
    15841 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
    15901 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
    15961 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
    16021 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
    16081 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
    16141 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
    16201 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
    16261 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
    16321 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
    16381 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
    16441 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
    16501 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
    16561 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
    16621 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
    16681 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
    16741 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
    16801 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
    16861 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
    16921 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
    16981 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
    17041 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
    17101 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
    17161 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
    17221 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
    17281 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
    17341 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
    17401 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
    17461 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
    17521 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
    17581 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
    17641 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
    17701 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
    17761 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
    17821 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
    17881 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
    17941 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
    18001 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
    18061 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
    18121 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
    18181 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
    18241 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
    18301 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
    18361 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
    18421 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
    18481 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
    18541 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
    18601 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
    18661 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
    18721 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
    18781 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
    18841 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
    18901 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
    18961 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
    19021 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
    19081 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
    19141 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
    19201 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
    19261 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
    19321 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
    19381 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
    19441 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
    19501 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
    19561 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
    19621 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
    19681 tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
    19741 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
    19801 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
    19861 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
    19921 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
    19981 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
    20041 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
    20101 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
    20161 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
    20221 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
    20281 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
    20341 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
    20401 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
    20461 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
    20521 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
    20581 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
    20641 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
    20701 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
    20761 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
    20821 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
    20881 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
    20941 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
    21001 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
    21061 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
    21121 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
    21181 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
    21241 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
    21301 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
    21361 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
    21421 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
    21481 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
    21541 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
    21601 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
    21661 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
    21721 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
    21781 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
    21841 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
    21901 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
    21961 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
    22021 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
    22081 cctccatatt atgttggctc tttattccca ctctgttttc aacgtaaagt gtataaaaca
    22141 gaatgcgata ctatcaaggg agttaaaaca atatacagaa cagtaaaaca ctataaataa
    22201 aaataaaagt gcaataaaag tgcaattttt gcacctaatc atactctgtt tatatatgaa
    22261 aactagattc tgaatccaat atttggttaa cgagaggcgt tcgaatttga acgtctttta
    22321 tatattactt tcaaataaat atgaggtaga atactatcta ttttcttttt aacccaattg
    22381 ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc cttctgtaga
    22441 atagagttac cacaacacat tcacagaaga gaggacttcc ctatgaacga ttttactaca
    22501 gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg tgtccatttg
    22561 gagaaagcag tcaatacgct tctcaaaacg gagttaacgg ctttcctaga ttacgaaaag
    22621 tatgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga ccgtacggtc
    22681 aagaccgagt atggggaact tcatctccag attccgcgcg accgcaatgg tgagttcaag
    22741 caacagactg ttcctgctta tagacggacg aatgacacgt tagaggagac cgtcattcac
    22801 ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa aatgtatggg
    22861 cattactaca cgccccaaac catgtccaat ataacaaaat catttacaga agaggtaacg
    22921 gcgtttaatg ggcgggagct tcatgaccgt tatgctgcta tttatatgga cgcaacgtat
    22981 attccgttaa agcggaaaac cgtcgccaag gaagctattc atatcgcagt tggcattcgc
    23041 ccggacggat caaaggaagt attgagctat gcgattgcac cgactgaatc catcacgatt
    23101 tgggaggaaa ttttattgga ccttcaagag cgcggtttga aaaatgtcct cctgttcatc
    23161 acggatggct taaaggggat ggtaggagcg attagtcggt tctatcccaa agctcgtttt
    23221 caacattgtt gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt cgatgatcgt
    23281 aaggaagtct gtgatgattt taaaatggtg tatcaagcct catctaaaga ggtggcgttg
    23341 gaagcacgtg gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt ggttgaatcg
    23401 attctttcga acgatcactt gctcactttc tatgatttcc ccttggccat acgcaagagt
    23461 atttattcta cgaacttgat tgaatcctt
//
LOCUS       DACEKQ010000044        23365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEKQ010000044 DACEKQ010000000
VERSION     DACEKQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.047"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(110..181)
                     /locus_tag="IW671_001526"
     tRNA            complement(110..181)
                     /locus_tag="IW671_001526"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Arg,seq:ccg)
     gene            241..1575
                     /gene="rpoN"
                     /locus_tag="IW671_001527"
     CDS             241..1575
                     /gene="rpoN"
                     /locus_tag="IW671_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0898514.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1778..2815
                     /locus_tag="IW671_001528"
     CDS             1778..2815
                     /locus_tag="IW671_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0898515.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            2856..3857
                     /gene="gap"
                     /locus_tag="IW671_001529"
     CDS             2856..3857
                     /gene="gap"
                     /locus_tag="IW671_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0898516.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            3967..5157
                     /locus_tag="IW671_001530"
     CDS             3967..5157
                     /locus_tag="IW671_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0898517.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5261..6016
                     /locus_tag="IW671_001531"
     CDS             5261..6016
                     /locus_tag="IW671_001531"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0898518.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            6132..7430
                     /gene="eno"
                     /locus_tag="IW671_001532"
     CDS             6132..7430
                     /gene="eno"
                     /locus_tag="IW671_001532"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0898519.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            7829..8968
                     /locus_tag="IW671_001533"
     CDS             7829..8968
                     /locus_tag="IW671_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0898520.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(9035..9610)
                     /locus_tag="IW671_001534"
     CDS             complement(9035..9610)
                     /locus_tag="IW671_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0898521.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(9615..10589)
                     /locus_tag="IW671_001535"
     CDS             complement(9615..10589)
                     /locus_tag="IW671_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0898522.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEPICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(10613..10657)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            10872..11840
                     /locus_tag="IW671_001536"
     CDS             10872..11840
                     /locus_tag="IW671_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0898523.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            11937..12230
                     /locus_tag="IW671_001537"
     CDS             11937..12230
                     /locus_tag="IW671_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0898524.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            12348..12584
                     /gene="secG"
                     /locus_tag="IW671_001538"
     CDS             12348..12584
                     /gene="secG"
                     /locus_tag="IW671_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0898525.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            12696..13442
                     /locus_tag="IW671_001539"
     CDS             12696..13442
                     /locus_tag="IW671_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0898526.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            13445..15802
                     /gene="rnr"
                     /locus_tag="IW671_001540"
     CDS             13445..15802
                     /gene="rnr"
                     /locus_tag="IW671_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0898527.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            15824..16288
                     /gene="smpB"
                     /locus_tag="IW671_001541"
     CDS             15824..16288
                     /gene="smpB"
                     /locus_tag="IW671_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0898528.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            16590..17309
                     /gene="atpB"
                     /locus_tag="IW671_001542"
     CDS             16590..17309
                     /gene="atpB"
                     /locus_tag="IW671_001542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0898529.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            17362..17577
                     /gene="atpE"
                     /locus_tag="IW671_001543"
     CDS             17362..17577
                     /gene="atpE"
                     /locus_tag="IW671_001543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0898530.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            17668..18192
                     /gene="atpF"
                     /locus_tag="IW671_001544"
     CDS             17668..18192
                     /gene="atpF"
                     /locus_tag="IW671_001544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0898531.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            18179..18721
                     /locus_tag="IW671_001545"
     CDS             18179..18721
                     /locus_tag="IW671_001545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0898532.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            18744..20300
                     /locus_tag="IW671_001546"
     CDS             18744..20300
                     /locus_tag="IW671_001546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0898533.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            20314..21216
                     /locus_tag="IW671_001547"
     CDS             20314..21216
                     /locus_tag="IW671_001547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0898534.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            21239..22645
                     /gene="atpD"
                     /locus_tag="IW671_001548"
     CDS             21239..22645
                     /gene="atpD"
                     /locus_tag="IW671_001548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0898535.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            22659..23081
                     /locus_tag="IW671_001549"
     CDS             22659..23081
                     /locus_tag="IW671_001549"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0898536.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         7865 a         4236 c         4990 g         6274 t
ORIGIN      
        1 atctatacag atacaacaaa aaactgtcca ccaattctct ttggcggaca gttttcactt
       61 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
      121 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
      181 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
      241 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
      301 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
      361 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
      421 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
      481 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
      541 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
      601 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
      661 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
      721 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
      781 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
      841 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
      901 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
      961 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
     1021 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
     1081 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct ccaacgtggg
     1141 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
     1201 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
     1261 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagattt cggtgtattt
     1321 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
     1381 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
     1441 aagccgttgt cggaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
     1501 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
     1561 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
     1621 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
     1681 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
     1741 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
     1801 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
     1861 tatctattgg atgcagccga ttgggcgtcg aagtttatcg gaaagtatgg ggatcacaga
     1921 acgagtctta cgaacagaaa cagacctttt gaaaaatctg aatttgatcg acacctcaaa
     1981 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
     2041 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
     2101 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
     2161 cgaagcgctg agcgaagcct tagaccgact acttccagaa ggcgagaata tcatcgctgt
     2221 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
     2281 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
     2341 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgcg cactttatgt
     2401 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
     2461 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
     2521 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
     2581 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
     2641 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
     2701 cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
     2761 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
     2821 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
     2881 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
     2941 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
     3001 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
     3061 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
     3121 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
     3181 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
     3241 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
     3301 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
     3361 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
     3421 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
     3481 actcaacagg tgctgctaaa gctatcggtt tagtaattcc cgaattgaac ggtaaattag
     3541 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
     3601 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
     3661 cttacggata caacacagac gaaatcgttt cctcagatat cgttggtatg acttacggtt
     3721 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
     3781 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
     3841 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
     3901 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
     3961 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
     4021 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
     4081 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
     4141 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
     4201 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
     4261 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
     4321 cgttttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
     4381 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
     4441 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
     4501 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgcccaat gattgcaatc
     4561 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
     4621 gataaaatct taatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
     4681 atcggtaact ctctagtaga agctgataaa gtagaactag caaaagaatt aatcgaaaaa
     4741 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
     4801 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
     4861 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
     4921 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
     4981 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca
     5041 gcagaacaat taggattcgc tgacaaattc acacacatct caacaggtgg tggcgcaagc
     5101 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
     5161 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
     5221 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
     5281 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
     5341 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
     5401 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
     5461 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
     5521 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
     5581 agacatcaat aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg
     5641 cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
     5701 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
     5761 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
     5821 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
     5881 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
     5941 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcattct tgtcattgtt
     6001 agacgctgta aaataattgt tgaaaatttg tgaaaaatgc aagaaatgtt tgcactaatg
     6061 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
     6121 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
     6181 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
     6241 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
     6301 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
     6361 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
     6421 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
     6481 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
     6541 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
     6601 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
     6661 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
     6721 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
     6781 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
     6841 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
     6901 tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
     6961 aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa aacgactggg
     7021 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
     7081 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
     7141 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
     7201 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
     7261 caatctctga tatcgctgtt gcaacaaacg ctggccaaat caaaactggt tctctatcac
     7321 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
     7381 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
     7441 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
     7501 gaactctcta cttgctgaaa tatcagcagg tagagagttt ttttgcaact agcttctgta
     7561 tatgatgttt acaaagaatg gtacaagaaa agatcatcct tgaaaaagga aagctaacaa
     7621 taactagcat catataagag cttttaagga tagaacagta gaataggaat ctatgagcaa
     7681 gtcctctcgt ataaataaaa taggagggaa gtagcagcat atttttcata aagtggctct
     7741 ctttcttcat ttttactttg gttgctccta tttgaaaatt cgagcacact caaatacaat
     7801 aaaaagaaat ggcaagggag aggattagat gaaaaaagaa gaaaaaatcg cgattttaca
     7861 agagatcatc cgaattaaat cagtgaatgg aaacgaaggt gaagtagcag cctatttgaa
     7921 taagcttttg gcaaaacatg acataacagg agagattgtt tcttacagag atggtagaga
     7981 taatttgatt gctcgttacc aaaagggcca atcaggaaaa gtgttaggcc tatctgggca
     8041 tatggatgtc gtggcggctg gtgatgaatc gtcttggacc tatgcaccct ttgcagcaga
     8101 aatccatggg aaccgtttgt acggacgtgg tgctacagat atgaagagtg gcttggcagc
     8161 aatggtgatt gccatgatcg aactgaaaga gtcaggaaaa ccatttaacg gaacagtcaa
     8221 attattagcc actgtcggag aagaagtggg tgaactaggt ggggaacagc tcacgaaagc
     8281 aggctatgta gatgacttag atgccttgat catcggagag ccaaccaatt acagtttgat
     8341 gtatacgcac atgggatcaa tcaactatac agtcacctcg cacggaaaag aagcgcatag
     8401 ctctatgcca gaccaaggtt ataatgcaat caatcaccta aatgaattca ttacgaaagc
     8461 aaatgctgaa atgaatcacc tcgctgaaac aatcgagaat cctgtcctag gaaaaacaat
     8521 ccataatgtt acattgatca gtggcgggaa tcaagtcaac agcatcccaa gccatgcgca
     8581 gctccaaggg aacattcgat cgattccaga atacccaaat gataaaatca ttgcattgct
     8641 gcaatcaatc gtcaatgaat tgaatcaaga gacagactat catttggaat tgatgatcga
     8701 ttacaacaaa atacctgtca aagctgatcc agattctcct ttgattcatt gcattcagca
     8761 acaattcagc cagccattac ctttagttgg agcggcagca acaacggatg cagccgaatt
     8821 tacgaaagct aaccattcct tcgactttgt cgtctttggg cctggtgtcg ttactttgcc
     8881 tcatcaagta gatgagtatg ttgaaatcga taattatttg gatatgatcg aaaaatatca
     8941 aggaattatt ttatcctact tagcgtaaga tttttttaat aaatgaaaaa acagagccag
     9001 attagcatta ttttgctaat ccagctctgt ttattcagcc tatttttctt tttgaatata
     9061 tcaagcgttg tggcaacata gtgatgacta atgaaacaac gacgatagac aacaaacgat
     9121 caaggtagtc tgtacctgcc tgtaccaaga tcacacttgt tgtctgactt aaacctaacc
     9181 catgtaatag ctgaacgatc atactggatc ctgaggaggt gatcccgcca aataactgaa
     9241 cggtaatgat cgaagaaacg atcgttccag gtaccgacaa aatcaaagcg agtaataaca
     9301 gtttcaagct gtttttatct ttgccgagat atcttggcaa aatcaatcca gcaagcaagc
     9361 ctgtgagaat ttggattggc gaataataca gagaaaaaat atctgtagtc actccactta
     9421 aaataccgct ggacaatcct gtcagcattc caggtacagg cccaagcaat gcaccagcaa
     9481 aaatcgttcc gatagaatcg agatagattg gcaaacgaag ccataaagca atcgttcctc
     9541 caatataatt gatcccaata cacaaagcca tcactgtaag tacgcgagtt gccattttgt
     9601 ttttattcat tttgttagcc catctttaac ctttccaaat ctttcataat cgtttcttct
     9661 tgtgcattta aaagaacggt taaaaacttt cggaaaaaaa gacgagtatc tacttttgtc
     9721 ataattttac tgttagcagg tttctgccaa aattcataac gatcaaccaa tgtttgtcca
     9781 cgacttattc cttgagtttc cacagcagtg aagcttttga aaccttcaca gatgggctcc
     9841 tcgataaaat aagcaacagc taacggatca ttgatgacac atcctaaaat tctttcttgt
     9901 ttccagtgga agtcaaaata aaaacgagtg atcgctttta gatactctcc ctcttcagga
     9961 ttcatttgac agcagtattc gaggatagtc ggtgtcaaga cgatttctct tgtcacatct
    10021 aacccaacca tttctatcgt ttgtttgaga ttctcgaata cataagaagc tgcttcagga
    10081 tcacaccagt aattgtattc agcaactggc gaacagttac catggctttt gtaagttcca
    10141 cccatcgaga caaaacgttc catatgtttc ccaatatttg ggttgacttt caaagcagaa
    10201 gcgatattgg tcaaaggacc caaagcaatg actgatgtat ttgtcttttc tttgaaataa
    10261 tcagccaaaa aatcaacggc atggagaggt tctgcctgtt ttctcaagat catcgggaaa
    10321 tttgtttccc ctaaaccatc cataccatga gtgtcttgtg cactgatgaa aggttgcttc
    10381 aaaggttttt ccattccttg ataaacagga atgtctaaac gatcacagcg ttccaggcat
    10441 ttcagcacat tctgcgtacc tatatctgca gggacattcc cacaaactac ggtaatagca
    10501 actacttcga tatcagggga tttgacagct aatgataaag ctaaagtgtc gtcgatccct
    10561 ggatcgcaat caataattac ttttcgcatt atttccaatc tcctttcttt agagatactt
    10621 agttttttat tctgggaagt ttccgaacca gtccaagacg agcgtcttga ttataatgca
    10681 cgaaattagt atactggctt ttattgcgaa tgtaaatgct ctcttacaag taaatctatt
    10741 gaggagaact ttaagtaaat gatacaatga aaaagaaatg gaatttacgt gtgccgaaaa
    10801 taaagaatcc tatggatcgg tacattggat aatagataga ttatagtttg gcagaaagcg
    10861 gcgggtgtac aatggttatt ttttatttca ttcttattct gataattata gggatcagtt
    10921 accattttct ttcttatttt tatgcaaaga aattagtaag agcgggagta cagagaggat
    10981 atgactggta tgaagacact ggtcatcgtt tgatggaccc tgctgcagtc acgtcagtag
    11041 aaaaaaaacg acaaaaatta gaagaagagt gtgaaagctt ttgggagcag ggaacaccca
    11101 aaacaatcaa aagttatgac gggttgaaac tagctggtca gatgttcatc catccaaata
    11161 agcagaataa atgggtcatt tgcgtacatg attaccgaag cacagggaaa agagacatgt
    11221 ctcatatagg taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg
    11281 cgcacggaga aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa
    11341 ttgcctggat ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg
    11401 gatcaatggg agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca
    11461 aaggatttat tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa
    11521 gtaaaatcac tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga
    11581 aatacgccgg ttactcctta aagcaagcat cagctacgag acagcttggc agcaatcatc
    11641 tgcctttatt agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca
    11701 cgattcaaaa tgctacggca ggggataaag cactgctgct tgttccggaa gcagaacact
    11761 tggaagcatc gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac
    11821 aaagaataac gatgaattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat
    11881 tgtttataga tcaggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga
    11941 ggagtgcggc atatggaaat caaagaagaa aaaatcgtct tgctttattc aatgatgaac
    12001 aacaagagat cggagaaatg acatggtcag atgcgggccc agacatcatg atcattgacc
    12061 atacatttgt agatccagca tatcgtggtc aaaagttagc tgaaaagcta gtgtatactg
    12121 gagtagaatt agcaagacga gacgggaaaa agatcattcc tttatgccct tatgcaaaaa
    12181 aagagtttga aaagaaacca gaatatcaag acgtcttacg tcagtcataa gaataacagc
    12241 tgaaagtttt gaactttcag ctgttattcc atttatatag agttgtcacg gacttttatc
    12301 tatgataaag ttaaagagac gatatctttt gggaaggagg ctgcgatatg tataatttaa
    12361 ttttgggaat cgtgatcgtt ttatcgattg ttatggtcat tgcgattatg atgcagccaa
    12421 gcaagcaaaa tagtgcagcg agtgcattta ccggcggtgc ggatcaacta ttcggcaaac
    12481 aaaaagctcg cggctttgaa gctgtcatgc agcgatcaac tgcagttatg ggagctatct
    12541 ggatgatctt attatttgtc ctagcatatt tatcttcgaa gtagttgaat aagcctctcg
    12601 caaaacggcg ggcggctttt tttattgcaa agagaacgct taagaagaag ttatgaaaga
    12661 aactatggta aaatgaggcg aaagaggtga gacgagtgag acaattacca aaaccccttt
    12721 atgcaaaaca tggaaagcga gcggttttgc tgcttcacgc ttactcagga agtccaaacg
    12781 atgttcgaat gctcgcacga tttttggaaa aagcagatta tacagtttat gcgccgcttt
    12841 ttaaaggaca cggaacaatg gagccatatg atatcttgca agaaaaagca gaaagctggt
    12901 gggcagacac taaaaaagcc atccattttt tacagtctga gcagttttct gatattgccg
    12961 tattaggatt atcaatgggc ggcatctttg ctgtacgagc actagaagaa gaatcagtga
    13021 ttggcggcgg ttttttctgt tcgcctttgt ctcctgtaaa aacaaacgtg cctgaaaact
    13081 ttgaaaaata cgtacgtcaa gtgctgaaga cagcaggtaa atcagagaaa gaaatcaatg
    13141 aaaaagcagt tgcttaccgc tcgttagctg aacaacaatt aatggatata caagatcagg
    13201 cagcaatcgt agaaagtagg ttgtcagata tccaacagcc gatattttta gctcaagctg
    13261 ggaaagatga aatgatcgac cctaatggcg tatttgaaac tgcacgcaag ttgagccgtc
    13321 agcgagtgac acttcaatgg tatcctgaaa gtggtcatgt gattacagtt ggtacagcaa
    13381 gacgcgaatt agaaaaagac gtcttggaat ttttagaaaa attaccttgg aatgaggaat
    13441 aaatatgaca aaagaaacga taaaagagaa aattctcttt ttcatggaaa gcagtcgcaa
    13501 acgcagtttt tctatggaag aaatcgctga aggtttaggg ttccaaaaaa gcgatgattt
    13561 taaattacta gtacaaacga ttgctcaaat ggaaagagaa cagagtatag ttttcaataa
    13621 aaaaggaaaa gtgaaacttc ctttaaaacc aatcttgatc gaaggaacat ttcgagcaaa
    13681 tgaacgagga tttggatttg taacgattga tcccgaagaa gatgacatct atatcccaaa
    13741 agaagcgact ggttttgcaa tggatggaga caccgtagca atcgatatca tgaagcgagc
    13801 aaatacagat atggatcgcg gagcagaggg gaaagtagtc gagattcgag accgggcgac
    13861 tactcagtta gcaggggaat tcgttgctta ttcagaagaa gaaatacaag aaacagatct
    13921 ttacggcgtt gttatcccga aagatagaaa gctcaaccaa tttaaagtat atgcagcagc
    13981 agagggagtt cgtccagttg acggaagtat tgtgctgatc gaactgaccc attaccctga
    14041 aaaagggtat gcgactagtt tagaagggat aatcaagcaa gtgattggtc ataagaatga
    14101 ccctggcatg gatatcctct caattgtcgt ggctaatggt atccctacga aatttcctga
    14161 tgatgtattg tctgaagcag atgaagttcc agatcaaatc agcgaaaagg atataactgg
    14221 acgtagagat ttaagaaatc agctcattgt tacgattgat ggagaagatg caaaagatct
    14281 agatgatgca gttacggtta aaaaattaga taacggtaat tattttcttg gagtacacat
    14341 tgcagatgtt tcttactatg tgacacaagg cagtcaattg gacagagaag catatgaaag
    14401 agggacaagt gtatatttga ctgatcgagt aattccgatg atcccgcaac gtttatcgaa
    14461 tggtatctgt tcactgaatc cccatgttcc aaggctgacc atgagttgcg aaatggagat
    14521 tgatccaaat ggacaagtag tctctcatga tattttccca agtgtgatcc aaacaacaga
    14581 acggatgacc tacacagctg tcaatcaaat cttagaagat caagacgaag aaacgatgca
    14641 acgctatgct cagctcgtac ctatgttcca agaaatgggt gaattgcacc gaatccttga
    14701 aacgatgcga atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt
    14761 tgatgctgaa ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt
    14821 gatcgaatct ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa
    14881 tctgccgttt atttaccgaa tacatgaaca gccaaaagaa gaaaaaatgc agcgcttctt
    14941 tgattttgct tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa
    15001 agatttacag catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac
    15061 tatgttgcta agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg
    15121 cttagcggca gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat
    15181 cgtccatcgt ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg
    15241 ggctgcggaa cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga
    15301 aacagaacgt gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga
    15361 agaattcgat gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa
    15421 tacagtagag ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga
    15481 aaatcattta gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacagaaagt
    15541 gcgagtaaaa gtagtcaaag ctgatccaga tgaacgagca gtagattttg aacttgtttc
    15601 cgcagaagag gttacagcgt tggaacggcc aaaaacacga aatggaaaat caggaaaacg
    15661 cgaaggaaaa caagcaggaa aacgcccaga aagaaatggc aagaagaaca aacgatatag
    15721 cgatagtaag caaacgttcg gtaagaaaaa agggaagaag ccattttaca aaggtgttaa
    15781 aaaaaagaaa aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg
    15841 aaaattaatt gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga
    15901 agcagggatg gtcttgcaag gaaccgagat caagtcgata cgaaacagcc ggatcaatct
    15961 aaaagatgga tttattcgcg tccgcaacgg agaagctttc ttgcataatg ttcatatcag
    16021 tccttatgaa caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt
    16081 acacaaaaaa caaatcatcc ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt
    16141 tcctttaaaa gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcgaaagg
    16201 gaaaaaatct tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga
    16261 tcgaacatta aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt
    16321 ttgcttttac ttaatgataa aatttgttag catttaatct gataaaacat gattatcata
    16381 ggttttttct tagatcagga gcaccgtgta taaagaaaaa ttcgcttcgt ttttgactga
    16441 aataaaaaag tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg
    16501 ataccgtgtg gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg
    16561 tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg ttccacattg
    16621 gaccaatttg gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt
    16681 tcttcttagc ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg
    16741 cgatagaatg ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag
    16801 aattgagcaa tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca
    16861 acatcggttt agtaacaaaa atcgttttac ctaacgaaac aacactttgg aaaagcccga
    16921 cagcagaccc gtttgttact ttaactttgg catttataat gatcttactt actcacttat
    16981 ttggggtgaa aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccatata
    17041 gttttatgtt tccgatgaaa ctgattgaag aatttacaaa cttattaaca cttgctttac
    17101 gtctttatgg gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatggtaa
    17161 taagcttagg ctggttctca ttgccgttag caattccatt agagatggtc tggatcgcat
    17221 tctccttgtt tatcggaagc atccaagcat ttgtcttcgt tactttatct atggtttata
    17281 tgagccataa gattgaagtg gaagaataat attcgtttta aaaacaaact actatttatc
    17341 taatttagga ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca
    17401 gcaatcggag ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct
    17461 cgccaacctg aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtc
    17521 gaagctgttc ctatcttagg ggtagttgtc gccttgattc tagtttttgg tgtttaattg
    17581 aatcagtatt aaggcagaaa caatttggtt tgttactgct tttttacatg aaaactagaa
    17641 gtacttcaaa gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc
    17701 aatgcgatgt taggaaatgt catcgttgtc agcggctcat ttttgatact actggctctc
    17761 ttgaagcatt ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc
    17821 gccaatgatt tggattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa
    17881 cgcgaacaac aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa
    17941 gaaagtggag aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt
    18001 ctaaaaagta aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta
    18061 aaagacgatg ttgcagaact atctcttcaa atcgcggaaa aaatcttgaa caaagaatta
    18121 tctccagaaa tgcacgaatc attaatcaat caatatatcg aaggtctagg ttcttcaaat
    18181 gaaactagat aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat
    18241 cgattcaaat caagcagaag aaatctatca agatctcgta agcttacgtc aaatctatga
    18301 acaagtacct ggattaggta atatgttaag tgatgttcgt ctagaacctc atgaaaaacg
    18361 agaaatcatg gacaagcttg ttagtggcta tgatggaatc gttaaaaact ttttagaagt
    18421 tgtttatgcg tataaccgaa tggatgatct tttgttcatg atcgaagaat atgaacgtcg
    18481 atatgatgaa catcaaggtt tgctgttggg aagtgtcaca acagcagttc cactttcaga
    18541 cgaacaacga atacaattag aaaagaatgt tgcaaaaaca atgaactatc aatcagtcga
    18601 attaaaacaa atcgttgatt cttctattat tggtggcgcg attgttgaag ctaaccatcg
    18661 agtaatcgac ggaagcattc gcacgcaatt agaaaatatg cgtaatcaat taaatagata
    18721 gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta
    18781 aagaacaaat tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt
    18841 atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc
    18901 ttgaattttc aaatggctca tacggaatgg cgcaaaactt agaatcaaat gatgtaggta
    18961 tcattatcct tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggaa
    19021 agatcatgga agttccagtt ggagaggcct tgatcggacg ggtagttaat ccgctaggtc
    19081 agccaatcga cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcaatgg
    19141 caccaggcgt tatgcaacgt aaatctgtta acgaaccaat gcaaacaggt ctaaaagcaa
    19201 tcgacgctct tgtaccaatc ggacgtggcc aacgtgaatt agttatcggt gaccgtaaaa
    19261 cagggaaaac ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct
    19321 gtatctatgt agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga
    19381 aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcgc
    19441 cgttgcttta tatcgcacca tatgctggta ctgcaatggg tgaagagttc atgtacaacg
    19501 gtaaacatgt cttgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac
    19561 tttccttgct acttcgccgt ccaccaggtc gtgaagctta tccaggggat gttttctact
    19621 tacactcacg cttattggaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa
    19681 tgactgcctt accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa
    19741 acgttatttc gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta
    19801 ctcgtccagc ggttgatgcc ggactatctg tatctcgtgt agggggttct gcgcaaatca
    19861 aagcaatgaa aaaagttgct gggactttac gtctagactt agcaagttac cgtgaattag
    19921 aagcatttac acaattcggt tctgacttgg atgcagcaac acaagcaaaa ctaaatcgtg
    19981 gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc accgctagct gttgaaaaac
    20041 aagtagtgat tttgtatgct ttgacacatg gattcttaga tagtatccct gtagacagta
    20101 ttttagactt cgaacatgaa ttattcgaat atctggatac caatcattca gatatctttg
    20161 aaacaatccg tacaacaaaa gatcttccag aagaagaagc gttgaacagt gcgattcagg
    20221 aatataaaga catgttctta gctacaaaag gaaataattc ctctacagaa gataaattga
    20281 aatccattca aaatgcatag tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag
    20341 acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc gggcaatgca gatggtgtct
    20401 gcttctaagt tgacaaaatc tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa
    20461 gtaagagaaa tcgttacgca tttgactgct actcagttaa atgatattgc ctcagaaaat
    20521 cctagagggg atatcaatta caacagcatg ctgctcagcc gtcctgttaa gaaaactggt
    20581 tatatcgtca tcactgcaga tggcggttta gtcggtggat ataacagctc gatattgaaa
    20641 caaacgatgt ctattttaga agaagaccac caatcacccg atgattacgt gatgatcgca
    20701 attggcggaa ctggtgcaga cttctttaaa gcacgcggta tcaatctagc atatgagctg
    20761 cgcaatctat ctgatcagcc aagctttgat gaagtccgca aaatcgtaaa tatggcaaca
    20821 actatgtacc aaaacgaagt attcgatgaa ttatacgtat gttataacca tcatatcaat
    20881 tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa tctcagactt agatccagaa
    20941 gaagcaacta ctttcgaaca agaatatatt tttgaacctt caaaagaaga aatcttgtct
    21001 cagttattgc ctcagtatgc tgaaagtttg atctacggtg cgatcgttga cgccaaaaca
    21061 gcagaacatg ctgctggtat gacagcaatg aaaacagcaa ctgataatgc tgctaatatc
    21121 attgatgagc tgacggtttc ttataaccgt gcaagacaag gggctattac ccaagaaatt
    21181 accgaaattg ttgccggagc ttcggcacta gaatagtatt gagattggag gaaaaaagat
    21241 gagttcaggc aagattgttc aagtaatcgg tcccgttgtc gacgtggaat tttctttaga
    21301 ccaatcctta ccagatatca acaacgcatt agttgtttat aaaaatgacg aaaataaatc
    21361 aaaagttgtt cttgaagcag cgttagaatt aggggacgga gtgatccgga ccattgcgat
    21421 ggaatcaaca gatggtttac aaagaggaat ggaagtcatt gatacaggca aagcaatctc
    21481 tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac gtactaggag atacgatcga
    21541 tttagaaaca cctttcccag aagatgcgga aagaagcgaa attcataaaa aagcaccagc
    21601 ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca gggatcaaag ttatcgattt
    21661 gcttgcccca tatttaaaag gtgggaaagt cggactattc ggtggtgccg gtgttggtaa
    21721 aaccgtactg atccaagaac tgatccataa tatcgcccaa gaacatggtg gtatttctgt
    21781 atttactggt gtaggtgaac gtactcgtga aggtaatgac ttgtattatg aaatgaaaga
    21841 ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa atgaacgaac caccaggtgc
    21901 acgtatgcgt gttgctttga ctggtttgac gattgcggaa tatttccgtg atgtagaagg
    21961 tcaagatgta ctgttgttta tcgacaacat tttccgtttc actcaagctg gatctgaagt
    22021 atcagccttg ttaggacgga tgccttctgc ggttggttat caaccaacat tggcaacaga
    22081 aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa ggttcgatca catcgatcca
    22141 agcaatctat gtgccagccg atgactacac tgacccggcg ccggcaacag cattcgccca
    22201 tttggatgcg acaaccaact tggaacgtaa attaacagaa caagggattt atccagcggt
    22261 agatccattg gcttcttctt caagtgcttt agcacctgaa atcgtgggag aagaacatta
    22321 taaagtagca acagaagtcc aacacgtatt gcaacgatat cgtgaattac aagatatcat
    22381 tgctatctta ggtatggatg aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg
    22441 cagagtccaa ttcttcttgt cacaaaactt taacgtggct gaacaattta ctggtcttcc
    22501 aggttcatat gtccctgtag aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa
    22561 atacgatgat cttcctgaag aagctttccg aagtgttggc cgaatcgaag acgtcgtaga
    22621 aaaagcgaaa acactaggct actagaaagg ggtgccccat ggatcaatat ttaacagtaa
    22681 atgtggtaac tcctgccggt ttggtctatg atcaccatgc agctatcgtc gttgcacgta
    22741 cgacagctgg tgagcttggg attttgccta aacatgcacc aatcatcgtt ccattgacga
    22801 ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca cgtcgattgg atcgctgtta
    22861 atggcggaat catggaagtt cgtgataatg ttgtttcaat cgttgctgat agtgcggaac
    22921 gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa
    22981 tcgaagaagc aaaagcaaaa caagatacga acgaattgaa acgggcaact gttgcgttgc
    23041 accgagcaat caatcgtatc aaagtatcta agcactcata aaatgagatg cggatgttta
    23101 tccgtgttta agaacagtaa aaagaggtta tgacagaagt ggtcagcttc aagacataag
    23161 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga
    23221 agctacttct ggaacaccgt atatcagagg ttgtaaaatc agcgttttgt cctgagtatt
    23281 agagaaacaa atggaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    23341 tcagatgatt gaacaccgtt tattt
//
LOCUS       DACEKQ010000045        23170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEKQ010000045 DACEKQ010000000
VERSION     DACEKQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.048"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW671_001550"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IW671_001550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0898537.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW671_001551"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IW671_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0898538.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW671_001552"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IW671_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0898539.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW671_001553"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IW671_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0898540.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW671_001554"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IW671_001554"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0898541.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW671_001555"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IW671_001555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0898542.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IW671_001556"
     CDS             complement(8053..8523)
                     /locus_tag="IW671_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0898543.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IW671_001557"
     CDS             complement(8569..8994)
                     /locus_tag="IW671_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0898544.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IW671_001558"
     CDS             complement(9061..9330)
                     /locus_tag="IW671_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0898545.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IW671_001559"
     CDS             complement(9341..10936)
                     /locus_tag="IW671_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0898546.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW671_001560"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IW671_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0898547.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IW671_001561"
     CDS             complement(14489..15049)
                     /locus_tag="IW671_001561"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0898548.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IW671_001562"
     CDS             15402..16376
                     /locus_tag="IW671_001562"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0898549.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IW671_001563"
     CDS             16546..17946
                     /locus_tag="IW671_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0898550.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IW671_001564"
     CDS             complement(18024..18746)
                     /locus_tag="IW671_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0898551.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IW671_001565"
     CDS             complement(18825..19331)
                     /locus_tag="IW671_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0898552.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IW671_001566"
     CDS             19454..21253
                     /locus_tag="IW671_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0898553.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IW671_001567"
     CDS             complement(21321..22895)
                     /locus_tag="IW671_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898554.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6354 a         5026 c         4228 g         7562 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt
//
LOCUS       DACEKQ010000046        23142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEKQ010000046 DACEKQ010000000
VERSION     DACEKQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.049"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..803
                     /gene="infC"
                     /locus_tag="IW671_001568"
     CDS             279..803
                     /gene="infC"
                     /locus_tag="IW671_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0898555.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            875..1075
                     /gene="rpmI"
                     /locus_tag="IW671_001569"
     CDS             875..1075
                     /gene="rpmI"
                     /locus_tag="IW671_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0898556.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1128..1487
                     /gene="rplT"
                     /locus_tag="IW671_001570"
     CDS             1128..1487
                     /gene="rplT"
                     /locus_tag="IW671_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0898557.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1739..3046
                     /locus_tag="IW671_001571"
     CDS             1739..3046
                     /locus_tag="IW671_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0898558.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3123..5252
                     /locus_tag="IW671_001572"
     CDS             3123..5252
                     /locus_tag="IW671_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0898559.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5227..5916
                     /locus_tag="IW671_001573"
     CDS             5227..5916
                     /locus_tag="IW671_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898560.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6324..7010)
                     /locus_tag="IW671_001574"
     CDS             complement(6324..7010)
                     /locus_tag="IW671_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0898561.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7146..7340)
                     /locus_tag="IW671_001575"
     CDS             complement(7146..7340)
                     /locus_tag="IW671_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898562.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7390..7830)
                     /locus_tag="IW671_001576"
     CDS             complement(7390..7830)
                     /locus_tag="IW671_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0898563.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7834..8679)
                     /locus_tag="IW671_001577"
     CDS             complement(7834..8679)
                     /locus_tag="IW671_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0898564.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8828..10246
                     /gene="pepV"
                     /locus_tag="IW671_001578"
     CDS             8828..10246
                     /gene="pepV"
                     /locus_tag="IW671_001578"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0898565.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10303..11250)
                     /locus_tag="IW671_001579"
     CDS             complement(10303..11250)
                     /locus_tag="IW671_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0898566.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11473..11823)
                     /locus_tag="IW671_001580"
     CDS             complement(11473..11823)
                     /locus_tag="IW671_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0898567.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12023..13102
                     /gene="pepA"
                     /locus_tag="IW671_001581"
     CDS             12023..13102
                     /gene="pepA"
                     /locus_tag="IW671_001581"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0898568.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13246..13566
                     /locus_tag="IW671_001582"
     CDS             13246..13566
                     /locus_tag="IW671_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0898569.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13588..14052
                     /locus_tag="IW671_001583"
     CDS             13588..14052
                     /locus_tag="IW671_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0898570.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14257..14862
                     /locus_tag="IW671_001584"
     CDS             14257..14862
                     /locus_tag="IW671_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0898571.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(14901..16190)
                     /locus_tag="IW671_001585"
     CDS             complement(14901..16190)
                     /locus_tag="IW671_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0898572.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            16618..17097
                     /gene="rlmH"
                     /locus_tag="IW671_001586"
     CDS             16618..17097
                     /gene="rlmH"
                     /locus_tag="IW671_001586"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0898573.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17182..17691
                     /locus_tag="IW671_001587"
     CDS             17182..17691
                     /locus_tag="IW671_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0898574.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17791..18441
                     /locus_tag="IW671_001588"
     CDS             17791..18441
                     /locus_tag="IW671_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898575.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18892..19830
                     /locus_tag="IW671_001589"
     CDS             18892..19830
                     /locus_tag="IW671_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0898576.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19843..20895
                     /locus_tag="IW671_001590"
     CDS             19843..20895
                     /locus_tag="IW671_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0898577.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            21161..21796
                     /locus_tag="IW671_001591"
     CDS             21161..21796
                     /locus_tag="IW671_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0898578.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21987..22778
                     /gene="msrA"
                     /locus_tag="IW671_001592"
     CDS             21987..22778
                     /gene="msrA"
                     /locus_tag="IW671_001592"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0898579.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7376 a         4068 c         4788 g         6910 t
ORIGIN      
        1 gaatcactgc tagaaagtct gctaatctaa tcattttcag aaaaggaaga caaactgtat
       61 gaaaaacatt gacaaaaaga ttagtttttg ctatactatg atggttgaga aataaaagca
      121 gaagcacccg cttctcgcct tagttgataa tgtcactggg ctagatagtt ttcactggtt
      181 ctattaggtg aagtacgcgc gggttctgta agagaactcg tttttttatt gccgtttatt
      241 cggtaagctt ttctttctct cataatattt ggaggtgaat gaccatagca aaggatatga
      301 tggttaacga cggcattcgt gcacgagagt tacgattgat cggttcagat ggtgagcaat
      361 taggagttaa gacaaaagca gaagcattga atattgcaga acaagcaaac ttggatcttg
      421 tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat catggactac ggtaaatacc
      481 gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa
      541 aagaagttcg cttaagtcct acgatcgact tgaatgactt caatacaaaa cttcgtaatg
      601 cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg
      661 ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg
      721 atattgcaac agtggaacaa aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg
      781 caccgaaaaa cgacagtaag taatttgaat gactaactgg ctgttggaca gccgatagtc
      841 ggataacgaa agattttgag gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg
      901 atcagcaaaa cgtttcaaac gtactggtaa aggcggattg aaacgcttcc gcgcgtttac
      961 aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt caattgcgta aagcaagcat
     1021 ggtttctagt ggcgacttca aacgtattcg ccaacaacta gcaaaaatga aataagaagt
     1081 acaaataaca gaatctgaag atttattttt attaggagga attatacatg gcacgtgtta
     1141 aaggtggaac agtaactcgt aaacgtcgta aaagagtgct aaaattagca aaaggttact
     1201 acggttcaaa acacacttta tttaaaacag caaaagaaca agtaatgaat tcatacaact
     1261 acgcatatcg cgatcgtcgt caaaagaaac gcgacttccg taaattgtgg attgctcgta
     1321 tcaacgcagc agctcgtatg aatggcttga gctactcaaa attaatgcac ggtttgaaag
     1381 ttgctgaaat cgacatcaac cgtaaaatgt tagctgactt ggctgttaac gatgcagcag
     1441 catttactgc attggctgac caagcaaaag aagctttggc taaataagct aaacaattat
     1501 aaagaggaaa gaagataacg aaaagttatg ttctttcctc tttttttatt tacaatcctc
     1561 catcaaatga aacaggacaa taacgtataa gtctaaaata gcataattaa ggttttttaa
     1621 ttgaaaactg aacatttatg gtcacccttt aatgaaagcg ctaactacat aagttagaat
     1681 gagtttgact gatatattag tttgcttatc agaaaaacaa aatggacgga ggaataacat
     1741 gagtgatatt attattggta ttttgttagt tatctcattc tttttcatgg tctggtactg
     1801 tgtaaaaggc tacaacttga tggtcggctt tgccatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccaatct tgatgtcatt aggtatttca gtaccggttg cactgttctc
     2221 atttatgggc tcgattatgg ctggaatctt tgctaatatc gtcaatttca aacaatatca
     2281 aacgatttat gctggtttca atccagcggc tgaaagctat acttataatg actatttcca
     2341 aattggtatg attgggatga ttgtaagctt agttgtggta ctaacggtcg ctaatatatc
     2401 aatgaacaaa aagaaacgct atgcaatggc agcaaacgta ccagctgaag gtggcgacgc
     2461 accaatgatc tcatggttag ccgtattatt accagtttta ggggtcgttc ttttagatct
     2521 tccaatcatc ttaggtttta ttctagcagg tatctgggca ttgctgttta ctggaaaatt
     2581 gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt
     2641 agatgttgca ccaatggttg gtttcttgat gaccttagcg atgttcaata attcagctgc
     2701 gtatgcttct ccttattttt cagctatttt tggagattgg ataccgcaat caccattagt
     2761 cttggcaatc gtatttgcta tattgacacc actaggattt ttccgcggtc caatgaatct
     2821 agtcggttca ggctcagcta ttttagctgt cgtattagct gttaatccaa cgatgtcgcc
     2881 agcctttttg ttcccgttat ttgcaatcac aacgattgct cctcagcatt tagatattac
     2941 acaatcttgg gttgcttggg gattaggtta tacaaaagta acttctcgag aatatatgaa
     3001 gaaatccatc ccaactggtt ggatcatcgg agcgatcttt gcctgatcac gttcttattg
     3061 tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg gaggaaaaga
     3121 aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg aaagcagtcg
     3181 caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca acccacgatg
     3241 atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg cgggaaaata
     3301 atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg actaatgcac
     3361 tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt ggattagaag
     3421 gttttttagc aaaacaacaa actagattga acgatatcga tttagggaat aagaaaaaaa
     3481 tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt gtttcagaaa
     3541 cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg gcatttggag
     3601 tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa tcgatcccta
     3661 caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact cggacagcag
     3721 caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca gaagattccg
     3781 tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc ggagtaatgg
     3841 aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca gcagcatcag
     3901 tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa gtaggcggaa
     3961 caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat tcaattgtag
     4021 gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg gcaggcggtt
     4081 cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct gcacatattg
     4141 ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct aaggttgaat
     4201 ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg gaagacggag
     4261 aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg caagaagaac
     4321 atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta gctgattatt
     4381 gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat gctaagattg
     4441 aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag atttctcttg
     4501 taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa atgggtgtga
     4561 aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca cttgcaatgg
     4621 tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata cgatcgttga
     4681 aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg atcgaagtcc
     4741 acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt tctacggaag
     4801 tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag cttgctgcag
     4861 aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct ttcttttatg
     4921 tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc gatcaaaaag
     4981 gatttatcaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca gccaattata
     5041 tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa ttgattgccc
     5101 gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg acggatctgg
     5161 aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag gaagaagtga
     5221 tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa cgaattacaa
     5281 cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt ttggaaaaag
     5341 attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg tatcgaaacg
     5401 gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg tgaacaaatg
     5461 gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac ttttgcaact
     5521 tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt aaaatgttca
     5581 ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca tgaactattt
     5641 caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat tgtgctgtgg
     5701 cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat agcagcgatg
     5761 agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga ggcagtctta
     5821 ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa aaaaatagaa
     5881 aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa aagtcgtgtc
     5941 acagtccttt ttttccgaat aaacgatgtt ctcctgtcaa ctcctcggtc acaagtcaaa
     6001 atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc ggagatgaac
     6061 ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
     6121 cagccaagtt aaaaacataa gaagctattt ttacttggct gttcttatta cttgatgcag
     6181 ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc agctgaccct cggtattagc
     6241 tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt tctttctcta atactcaggt
     6301 caaaacgctg attccaaaac ctcttatttg atgggtctgt cctgaatatc ttcaaaataa
     6361 tcggggtaat tatgaatcac gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg
     6421 caaagaaatc cgacttcatc aaagttatga tcaatcattg attgaagcat tgtctgatgt
     6481 tcatctaata ctcgacccca gtctaattct gaaatcgtca aacgtaacca tctgaaacgg
     6541 ttcaagtgcg tactataact ttctagccaa tcccaaattt ctcgttttcc agcaatttca
     6601 taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt
     6661 actgcctgat cggtttgttc tagtattttt tctagtttag cttgatcttg cttggtcatt
     6721 ttcgcactgc attcttgcag gatcggtttt tccaactttt cccgcgtata acgggctaat
     6781 gaagcagatc ggatatcgat ttttgaaaca taagtgccgg actgtggaat ggtttttaca
     6841 agctcttgat tgcgcagttg gatcagtgct tctcgaatcg gtgtacgccc gatagctagt
     6901 tgttcttcta acactttatc ggagatttta cttccaggtg ctaactcgga ataaatgatt
     6961 tgtttacgta tagattcata agcttgctgt tgcaaattat tgataaccat ttaaccttcc
     7021 tttctatgca gatataatct attataacat aaatcgctta caaatcgtgt gggcataaaa
     7081 acacagtctg caaaaaaagc cctgataggc atgcctatta gagcctcgaa aagaaaaata
     7141 ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac gaccgccgat tcttttagca
     7201 aaagcttcgg attctttttt gtctttgaaa atgagcagat tgtctaagtt tttttcccga
     7261 gtgcgacagc cagttttatc gatgtaaccg tgcattactt taacgacata catggggatc
     7321 acctgctttt ttcttttcat tatacgaact actcgacaca atacaagaaa aatgcttaga
     7381 cggtctttat taagaattta catcatccaa agaagcgtac gcttggaata actgatctaa
     7441 ctccggattg actttgctca gcttcactaa tcgttgcgaa tcagaaagta aaaaacagtg
     7501 tttactactg ccagtagttt ttaatttttt gtcttcagtt cccacaattt cgtagctaaa
     7561 atccaaccgt gtccccgtat acttttggat agttggatgg atgctcactt tttctccgta
     7621 atggatcatt tctttatagc tgcaatttac ttccagaacg gggatgatga tcccagattc
     7681 ttcgattttt ttatagggaa accctagatg taccaataag gctacccgag cctcttcaaa
     7741 ccagcggata taattgctgt gatgaataat ccccatttga tctgtttcat aataaaaagg
     7801 ttctcgcaca taacctgtaa atttctccat gactcatgcc tcgttttcca ttgatttttt
     7861 acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt tgtgtcggca aaacaatata
     7921 ttgttctttt gccagctggt ctccaatgaa actatgaaga taaatagctg ctgcaatcgt
     7981 ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc atcccagcta aagtgtcacc
     8041 catgccgccc gttgccatag cagcagaacc taaaggattg gtgtagaagt gctggtgacc
     8101 ataaattttt gtctcatgac ttttcaaaac gagcacagca ccgatttcct gttgcttctt
     8161 tttgttttct ttatcccgtt gttcttgtat cggtagagaa ctcaaccgtt gccactccat
     8221 ttgatgaggt gtaagtacag tttgttcggg atagggtagc aaaagatgat tagcagcaag
     8281 taaacttaaa gcagagccat caatcaccag ccattgttct tttgattggt tttctagtac
     8341 ttgtttgaaa cgttccaaac tcttagaaga agtacccaat ccgggaccaa tcaagagtac
     8401 atctgcttgc tttattacgg aaagaaactg gtcagtatcc gtccaaacta caatcattgc
     8461 ttctggcaga tgactgtgaa gagcactccg atttttttca tcagttacta cagtcgtgag
     8521 acccgctccg ctgtgtacgg cagctaaagc actcataatg attgctccgc catattgttc
     8581 attgccgcca atcaaaacga tacgtccaaa tgttccttta tgtgaaattt ttggacgagg
     8641 tgtgatgatt cttttcacta gttgttcgtt tatcagcatc taaaaacctc cttttactca
     8701 ttatagcgta tatttttttt agagaaaaac agataagaaa aggaattgtc agattttttg
     8761 aactattttt ttctgtaatt tgtggtatca tgaacacgaa gacttaaata agtggaggat
     8821 taactttatg acaattgatt ggcaaaaaga agtagaagca cgtaaagaag acttgcttga
     8881 agacttgaaa aatcttttac gtgtcaacag cgagcgcgat gactcaaaag taacaccaga
     8941 tgcaccattt ggacctggac ctcgcgacgc attgaaacat atgttagctt atggcgaacg
     9001 tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg catattgatc ttggcgaagg
     9061 agacgaaacg ctagggatat ttggacacat ggatgtcgtt cctgcaggag atggctggga
     9121 cacagatcct tatgaaccag taatcaagga tggaaagatc tttgcacgcg gatcaagcga
     9181 tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag atcattaaag aacttgactt
     9241 gaaactatca aaaaaagttc gcttcgtagt cggcagcgat gaagaaagcg gctggggcga
     9301 tatggcttat tatttcgaac atgaagaaga accagatttt ggtttctcac cagatgctga
     9361 attcccaatc atcaatggtg aaaaaggaaa tgtttcttta gctttacgct tcaaaggcga
     9421 caatgcagga gattatgtct tgaaatcttt tgtttccgga cttcgcgaaa atatggtgcc
     9481 aggaacagca actgcagcac ttcaagtacc aagtgcagat gcagctatcg cgatggaaga
     9541 agcattttac caattcatcg aagcaaatcc agtaagcgga acgattgaag cggacaatac
     9601 gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt gcaagcccac aatctggtat
     9661 caatgcaggt tctttcttgg cattgttctt agataactat gaattcctag gatctgctaa
     9721 acaatttatc catgtagcag ctgcttatgt ccatgaagat ttttatggag aaaaactagg
     9781 agtcgcttat gaagatgaaa aaatgggcaa attgacaatg aatgctggac tatttgcttt
     9841 tgaagaaaat ggtacagagg aagcgaactt catcaatatg aatttccgtt ttccaaaagg
     9901 tgtaacagta gacggattgc aaagtgacat cgaacaaaca gtggggcaag aaggcgcaac
     9961 agttactcga ggtgctcgcg taatggaacc acattacgta ccaatggatg atccattggt
    10021 ggctacatta ttgcaagtct atgaagacca cactggagaa aaaggctacg aacaaatcat
    10081 cggtggcgga acttacggac gtttgttgaa acgtggcgtt gcttatggag cgatgttccc
    10141 aggatatact gatacaatgc atcaagcaaa cgaatttatg agtcttgatg acttgttcag
    10201 agcaacagca atctatgcag atgccatcta tcgtttggcg aaataattaa ttaaaaagag
    10261 gatcaagaat tgcaaattat ttttgcagtc ttgatccttt ttttactttc tactgtggta
    10321 ttcatgataa acatgatata gattcaattc atattctttc ttagcgttcg tgacgactgt
    10381 atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa gtcatcaatc cagtacttaa
    10441 aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcgcgaa tcacaggaat
    10501 ccccgtgacc aatccaaata aaaagaagaa gatggtccaa atcccaaaga aaagcaaagg
    10561 cttataatct ttaaacattt taataatcat catgatcact ttgaatccgt cagagaatgt
    10621 attcaatttt gattcgctgc cttcaggacg atcacgataa tcgattggga tttcaacgaa
    10681 cttgaatttt ttatctaaag catggatcgt caattccgtt tcgatttgaa aaccagaact
    10741 cataatagga aaacttttga caaagatacg attgaatcct cgatagccgg tcatgacatc
    10801 aagaattttt gttttataca ttctagtaat tgtatttcta acaaggttat ttccaaaatc
    10861 gtggaaaggt ctcttattct catcaaagta ggttccattt gataggcgat caccaatgac
    10921 catatctgct tcaccagaac gtaatttctc gagcaaacca tgtacagctt ctgcaggata
    10981 agtatcatcg ccatctacca ttaaataata gtcggcatcg atatcaaaga acatttggcg
    11041 gatcacattt ccttttcctt gacgaggttc tttttttacg attgctccac cttgaacggc
    11101 caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg cttctggtaa
    11161 ttctcgttta aagtctgcaa tgactgttga aattgttgct tcctcattgt aacaaggaat
    11221 cagaacagca attttttctt gttgttccat ttttcactct ccttaactat agaaaacaaa
    11281 agctaaatta aaaatacctc ataatcaaag gaaatgctag tttttcccgc taaatatcat
    11341 ttatttaaaa agaaaatact ggaatatatg tttggaaagc tctatctaaa aaaccaccca
    11401 ctacaaaaga aggacatgat acctctcttt tgaatagtgg gtgcgtattc gttctctcac
    11461 ttttttgacg tattaatctg ttgcaccttt ttttcttgaa atttctaata cggttcctgt
    11521 atgagcatct gctaaaaatt cataataaac agtttcgcca tcttctattc gagaaagacc
    11581 tccccgataa gcactcgacc gaacggcgaa tttcctaata ggttcctttt caaaagagat
    11641 ccaagaacct tcgattgcac cttcttttag aaaagatgac ttgatatttt caagtacttg
    11701 gtctgctgac aatacttttt tgcgatgata ccaaatcgtt gaagctaccc cgctcaataa
    11761 tcctagaccg attccaactg ctaacccgcc tttaaaatag cttagttcgc tttcttcata
    11821 catgtggaca tacctccttc ttctcacttg ttactccttg tgcttatttt accacactct
    11881 catattttct ttgaaataaa taaccgctac cagaaaaaat aagctataat aaatacgatc
    11941 ggtctgcaga aaaaatcata ttttatccgc agatcaatat aagtctgaga ggagtttagt
    12001 ctgaaattta gacgaatagc atatggaaga aaaaacattt caacgaatca aagagttaac
    12061 agaattacaa ggaacaagcg gttttgaaca agatgttcgc gcttatatgg aaagtcatat
    12121 ggaaccgctg gtagatgagt tacagttaga tggtttgggc ggcatttttg gtctgcgcca
    12181 tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca catatggatg aagtcggatt
    12241 catgctgaca caaatccaag aaaacgggct ttttaaagtc gtacctctag gcggctggaa
    12301 tccttacgtt gtttctgctc agcgttttac acttaaaacc tctactggta agaattatcc
    12361 atgtatttct tcttccgttc ccccacattt attgcgagga acaagcggac aaaaatcagt
    12421 agaagtaacc gatattttgt tcgatgctgg gtttgaatca agagaagagg caatgagtta
    12481 cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa acgatcaaaa cagccaacgg
    12541 aaagaatatt atcagtaaat catgggacaa ccgctatggc tgtacaatgg tattagaagc
    12601 tttagaagcg ttgcaaaatg aaacattagg tcatacattg attgcaggcg cgaatgtcca
    12661 ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat aaattcaagc cagatctgtt
    12721 ctttgcagta gactgctctg cagcagatga tacagtgacg aaaaatggca cattcggaca
    12781 tttaggagaa ggtacattga tgcgtatcca agatccaggt ttgatcatgc ttcctcgttt
    12841 gcgcgagtac ttgctggata tcgctgaaac aaacaatatc ccttatcaat actttgtttc
    12901 aaaaggcggt acagatgcag gtgctgctca tacacaaaac gaaggaatcc caagtacagt
    12961 gattggcgta gtgggacgtt atatccatac gcatcaaacg atgttcagta ttcgcgattt
    13021 tgaagctgca cgagaaatgt tgattcaaac actgaaaggt ttggataaat caacagtgaa
    13081 tacgatcgta tacggaaaat aatgcgatag tgaaactata aaatctggca aaagattgct
    13141 ttttgccaga ttttttttat aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt
    13201 ataatttaat tgaaaaatac ataaacagaa agtagaggac aaagaatgat tatcccaaat
    13261 agtttagaag aattagcagg ctacgtagaa actggaaaga gtgtattttt ctttactgcc
    13321 ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc ctgaaattga aaatgacttt
    13381 tctgattggc aatttattga agtagaccgt gacaagtaca tagatgtagc cgctgaatgg
    13441 aacatttttg gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta
    13501 gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag tgtaacaaaa
    13561 ggctgacatc tgttaggagg ggtcaagatg aatcaagaat ataagaatat tttagtaggt
    13621 atcgacggta gtgaacaagc acttgctgct ttcaaaaaag cggtagaagt ggctcgccgt
    13681 aataatggga aagtatatgt agcaaacgta atcgatcagc aattttataa ctttatgggc
    13741 tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa ggaaatgatc
    13801 gaagaatgta aagaatatgg gaaatctgtt gattacacaa atatagaagg aattattgca
    13861 tatggttctg ttcgtgaatc catggcagtc aaattgccaa aaaaatatga gatcgatcta
    13921 atcatggttg ggcagtcagg tctaaatgca gtcgaacgct ttatgacagg aagtgtagcc
    13981 agctacgtga tacgtcattc accttgtgat gtcttgatcg tttctgacga agaagaacag
    14041 ccaagtaatt aagcaagtgg aacttgtacg acctttttaa tcaattaact aaaacagtaa
    14101 agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt gttccagaag
    14161 aggcttcttc ggaaataaag catgaaaaaa atttcatgca gtgaatgcaa ttttttgtta
    14221 agataaatct cagcaaagga taatggagga aagaacatga tttttgctta taacaaagca
    14281 cacgtcggag acaccttgat ggttattata gccgacgata aaggaacaga aaacactgtt
    14341 gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag gtcaagttgt tgcttggaac
    14401 tttttccata tctccgatca tctaacaatc gaagggaacg gccaagtaag cttaacagat
    14461 gaggaattag cgaaattgaa tcgattgatc aaagaagcag gattcgaaga acagcttgaa
    14521 gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa catgcgtccc acatcctgat
    14581 tctgaccatt tatcgattac tgagacagaa gtagacaatg gtacagtatt gcaaatcgtt
    14641 tgcggtgcac ccaatatcga agctggacaa aaagttgtcg ttgcaaaacc aggagctatg
    14701 atgccagatg gaatgatgat ctggccagga actttgcgag gggtagatag ctatggaatg
    14761 atctgttccg ctcgtgaatt gcatttgcca aatgctccag caaaaaaagg aatcttagta
    14821 ttgcctgagg atgccgtagt cggagaagca tttccaaaat aaagaggttg tgacaagtct
    14881 ttgtcacaac ctctttttta ttaattttca ctattttgat tttgttttaa tgccgattga
    14941 tcgatcgtca attcaacagt agttgttttt tctttcgagc cttcatagta agtgattttg
    15001 acagaatctc ccactttttt cttatacaaa gcagattgaa gttctacacc agaagaaact
    15061 tctttgtcat cgatcttagt aatgacgtcg tattgtttca atccagcttt ttcagccggt
    15121 gtagctgttt ggacatttgt cacgatgaca ccgttagtta cagatgaagg aatcttcaag
    15181 atttgttctt gttgctgagt agaaacggca gataagtcga ccattgtgat acctagagct
    15241 ggacgtgtca cttttccgtc tttttccagt tgattgatga tattcactac atcgttactt
    15301 ggaatagcaa atcccattcc ttctacgctt acattagaag tagattcaga agtacttgcg
    15361 atcttacttg agttgatacc gataacttgt ccttcgatat taactaaagg accaccagaa
    15421 tttccagggt tgattgctgc atctgtttgg atcgcattga tattgactgt ttcgttcgat
    15481 tcattcgtac ttgtcacttg tcggttcaag gaagaaatga ttcctgaagt gacagaattt
    15541 gcatattcag atcctagtgg ggaaccaatc gcaatcgcag gctcaccaac cttcagagca
    15601 cttgagtcac caaaagaggc aactgtttcg actttgtctg aattgatctt caacaccgct
    15661 aaatctgaat aagcatctgt tccaactaat tcagctttta ccttcgtacc gtctttcatc
    15721 aagacttcaa gaccttgctg gccatctact acgtgattat tcgtgactac ataagcagta
    15781 ttcccgtctt ttttgtagat cacgccgctt ccttcactat aagcttccaa attactatca
    15841 tctgagctgc tactttcttc ttgctgcccg aatagtccgc caaaaccgct tgattggttt
    15901 tgactttgta agttgataac agaaacgaca gcgtcttgaa ctttatctac cgctttggtg
    15961 atatcagaat ctacattgac tttgatattt tccacaactg tttcaccggc agaattctga
    16021 ttgccgcttg tagcagtaga actattgttg ccagtgccca tcgctgcata aaaaatgcca
    16081 gctgtcagta gtcctccaac gatccctcca acaagcccca gacctaactt acgccagagg
    16141 ctattatttt tacttttttt catctttggt gtcacatttt ttctatccat gtgttcctcc
    16201 tccaattaca ttcatttatc tactgttagt atagtcttat ttgttgactt tagtctactt
    16261 tgagactgtg aactttttat gaaaaacttt aggaaaaatc attgttttca gatgaaaata
    16321 tttgattttc tttttccaca gatactgtgt aaaactacgc ggataggatt ggaaaaataa
    16381 gggataaaac atatccacag cctgtgtata aagtggataa attcttataa aacgtatgtt
    16441 tgcctaatga atgtgatgaa ataaggtaaa ataacactgt tttctgtgga taaacctgtg
    16501 gatgttgtgc ataactgttg gggaaatctt tttaacaaaa atagccaaaa taaacatata
    16561 gacgaaaaaa ttgattataa taaacaaagt gagcatgaag accaaaggag agaaaacatg
    16621 aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat acctgatcca aggtatcaat
    16681 gagtatgtca aacggctgaa tgcctatgca aaaatcgaat tgatcgaagt tcctgacgaa
    16741 aaagccccgg agaatttaag cgaagcgcaa atgagacaag taaaagaaaa agaaggggaa
    16801 cggatcttag cgaaaatcaa agaacaagaa tacgtctatg ccttagcaat tgaagggaaa
    16861 aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat taggtatcca aggaaaaagc
    16921 caccttgttt ttgttatcgg cggctcttta ggactcagcg aagccgtcat gaaaagaagc
    16981 aatgctcaga tctcttttgg aaaaatgacg tatccacatc aattgatgcg tctgatcctt
    17041 gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg cgccttatca taagtgatgc
    17101 ggtttttatc attgatccta aaaatcttag caagacaaaa agtacgtaag aaagtaaaga
    17161 ctaaatactt cggaggaaca aatgaataac aatgataggt tattgcgatt gcgttatgcg
    17221 attgatttga aagatacgga tttgattaaa gcttttgagc taggcggtgt accactgact
    17281 aaagaagaag ctcaggcggt actgaccaaa gtacaggata agaacaaaga taatgcagaa
    17341 aataatgtgt atgaaaaaac catcaacaac caagtttttg acgcattcct aaatggcctg
    17401 attctcttgg cgcgtggtcc gcaaaaagat aagccgatag tagacacgcc gcaaaaaagc
    17461 aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat taaaaatcgc cttgtctatg
    17521 accactgatg atattttaga cgtttttgct gaggcagaaa tctatccatc aaaaggcgaa
    17581 atcggggcat ttttgagaaa agaaggccaa cgaaatttta agccttgcgg agataaatat
    17641 atgcgtaatt ttctcaaggg gttaggaatt tataatcgga gaaaagtata aagaaatttc
    17701 atgagaaatc aaaacaatgg ttgttttgat gagagctagt ttttgtatga gattaaaata
    17761 ttatcttaat cgaggagctg gattggtttt atgaatgatg aatataagaa tgatgaagat
    17821 aagatgcttt ttgaagagat agagaatcga tgtaggctca attttgagct tcggggaaag
    17881 atgtctttaa tacaacaaaa aaagtattta gcgaataaat cagagtttac tctgggacat
    17941 gttgaaaaat taatctcgga ttggattagt agtagaagcg aatttacaaa aataaaacag
    18001 ccgattaaat ttgacatgaa aaaacttttg ttaaataaga gtgagatagg taatagagat
    18061 caatatatta gagctaaagg gcaagaaata attgattcat taggtgaaat gaggtcatac
    18121 aattatttgt atgtaacaca tagagctgat ggaatggtca ttactgtggg gaaatcttct
    18181 tctaatgata tttttttaga tggagatctt ttttatcaac tcaatacaaa tcacttgtct
    18241 ggcactgaaa atataatttt aaggacggaa tatggcaatg aaatatttgc aaaatatgat
    18301 gagattttaa aaaattattt ggactgggct tggataattc cggttgagtc aggagatgct
    18361 aagaaactgg agagattatt gggcgatgaa ctaattaata aaaaagtacc aattttaaat
    18421 tattattccc atagacaata aaaggctatt tttcatgata atactagtta taactgtaaa
    18481 aatggaagaa aatctgggaa agaagttttt aaataagtgt attgttcgat tttaataaat
    18541 aataagcaat accatccgtc tgtgttgcgc aacatcgtac tacaatgaat attttttcac
    18601 aaaaagaaaa tcatccatcg aatgaatgga aggttttagt atacacgagg ttaatgcccc
    18661 ctcttttata atcagaggag tgaggttttt tctatgaatg tttctatttt cttattgcta
    18721 gtctagtttc tcttacgatg aaattgggat tctattccag tttttattga tttgctgata
    18781 aaataacttg aatggtggat ataaacatga taaataagag aagaataaca aaacaaggtt
    18841 gtgaaatgat tctcaaatga atataataga aagaaaaagg aggaagaatc aatgaaaata
    18901 gcaattgcag gcgctggcgc aatgggaagt cgctttgggt taatgcttca ccaaagtggg
    18961 aacgaagtat tattaatcga tggctgggca gaacatgtcc aacaaatcaa agagcatgga
    19021 ttacaagcaa attttaatgg taaagaggta gaagcaaaac taccaatcgt tcttcaatcc
    19081 gaagtagaaa aagaagatca agttgatctg attattctat ttaccaaagc gatgcagctg
    19141 gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag atacagaggt cttatgtcta
    19201 ttaaatggta tcgggcatga agatattatt gagaaatttg taccaatgga aaatatctat
    19261 attggaaata ccatgtggac ggcaggtctt gaaggtcctg gtcaggtcaa attatttgga
    19321 agtggttcag tagaattaca aaatttaggt gatggaaaag aagcagctgc aaaaaaatta
    19381 gcagacaaat tgtctgaatc aggattaaat gctcattttt ctgacaatat tcactattct
    19441 atttatcgca aagcatgtgt gaacggaaca atgaacggac tatgcacaat cttagacgtt
    19501 aatatggctg agctaggaaa aacatcgact gctcataaaa tggtggcgac gattgtcaat
    19561 gagtttgcca aagtagcagc agtagagaag attgaactag atgtcccaga agtcattgca
    19621 cattgtgaat cttgttttga cccggaaaca attgggttac attatccttc aatgtatcaa
    19681 gacttgatta agaaccatcg attaacagaa atcgattata tcaatggcgc aatttctaga
    19741 aaagggaaga aatatggtgt tgcgacgcct tattgtgatt tcttgactga gcttgtccat
    19801 gcaaaagaag atagtttgaa cgtaaaataa aggagagatt gaatggaaac ttcacaaaaa
    19861 atatcaccga aaatttttct gaataaagtc ctcgctggta cagcaacagg aatcattatt
    19921 ggattgattc ctaatgcagt attaggtgcc attttaaaat attttagtgc gtatcccttt
    19981 gcggtaacga tcgcacagtt agcagttatt tttcaactag caacaccttt gatcattggt
    20041 ggtttgattg ccttacaatt tggatttgat ccgatgagaa tgatggttgt agccggagct
    20101 tcttttgttg gttctggtgt agtcaaattc acacctgatt tgggtgaaac gggtgtttac
    20161 gttggtgctg gtacaggcga tattattaat acgatgctga cagcatcgat tgctgtcggg
    20221 ctgattttac tgattggaaa tagttttggg tcagtgtcga ttgtgttaac accgattgtt
    20281 gtaggtgtag gtgcaggttt acttggtttt tatttattcc cttttgtaac agcaattact
    20341 tctgcaattg gatcattgat taataacttc actaccttac aaccgctgtt aatgtctatc
    20401 ttgattggtt gttcctttgc ctttttgatt atctctccta tttcaacagt agcaatcgga
    20461 atggcaatcc aactagatgg tgtttctgct ggtgcggctg cgatgggcgt agctgcaacc
    20521 acttttgttt tagtagtgaa ctcttggaag gttaataagt caggtgtgac aattgcgatt
    20581 gctttaggcg ctatgaagat gatgatgcct aatctattta gaaaacctgt gattcttatt
    20641 ccttgtttag ttagtgcagt gattacagct attcctgtcg ctctattttc tatttcaggt
    20701 acgcctgctt ctgctggttt cggcgtggtc ggacttgttg gtccgttggc ttctttagat
    20761 gcagggctaa atggctttct tgttgttctt tgttggttag tcatccctgt ttttgcggca
    20821 ctaattactc aatttttatg cgagaaagta tttaaactgt atgatcgaga agaagtcttc
    20881 aaatttttag gctagtagat tttactaaga aattttcaat caatggatgc gtaagtgaga
    20941 tgtcaacaac atctgactta cgtttttttg cctttttgag taggagattg atgctgacaa
    21001 ctgaactctt cagtgccatt cgatttttta aaatgtagaa gaaactcacc tttatgttat
    21061 tgattgttca aaaggcaact agaataaaaa tatgtgttaa aatgagaaaa aatttgattg
    21121 ttggatgtat cgaataatca ctaaaaagta ggagaagaaa atgatttttt tgcaaaaata
    21181 tggtttttat tttttattat taggtgtgat tagtgattta tcgacaccgt atatccttgg
    21241 ccttttttat cccaaactga atcaaatgac taccgtaatt agtgtgtttg gtgacgtaga
    21301 tagtccagtc agacgtgctt tcttggtctg gtcagttgta tctggacttt tttttgttct
    21361 ttcgttgcct gcgttgtatc acctatttat tggaacatca aaaacacttg caatacttgc
    21421 tgttgcaaca gttggtttat atgggatagg tgattgtatt tttactgggt tattcagtat
    21481 caatacgaat gaaagctctt ggaatctatc gacttggatc cataatatag gttctgggct
    21541 aggctatgca ggctttctac ttttcccatt gttacttgtt ctgctttatc gtcaaagtgg
    21601 tcaaggtacg cttagccatt tttatcttgt tttaacagtt attagtttac taattgcagg
    21661 tctttacaga ttagcacgga ttccgtcgat tagtcagttt gccttcttta agcaattggg
    21721 cttctttcaa cgcttaagtt ttttctttaa ctatttgccg atgatttgtt taagtttttt
    21781 tcaactacga aagtagaaaa agcagtgtga agctgattaa gtaaaagaat ttctctatgg
    21841 gtaaccattc gtgtggatgt gttcgactaa gaatagagat gaggagagcg aaaagcaagt
    21901 tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag tcattttttg aatgaataag
    21961 aagaaaactt gaaagagggt gacttcatgt tagatgtgga gcagatggaa atagaccttt
    22021 ataatattat tttaaatcga aatacaagag aatgggaaag aacacaattt gaagaggcaa
    22081 aagaagacct agcaaaaatg tcgataaagc aggtgataga aaaactagag gtcaacttgc
    22141 gtccgctagc actacgagat aatttgacac cagatgcgat ggatttttat ttaagaatca
    22201 caggggagtc agcgagtgca aaggcttatg attatgccaa acataagata acttcaccct
    22261 tataccaaga acgggcgatt tttgccggtg gctgtttctg gtgtatggtt gagccttttg
    22321 aaacaaaacc aggtgtaatc tcagtgttat ctggttatac aggtggagag accaaaaaac
    22381 caaattacga tcaagtcagt ggtggatata ctgggcatgt tgaagctgtt gagatcatat
    22441 ttgatacacg tcaaataacc tatgaagagc tgttggagat ttattggcaa atttcagacc
    22501 ctacagatgc ctttggtcaa tttcaagatc gggggaaaca atatcgttca attctatttg
    22561 ttgttaatga aaagcaaagg caattggctg aaaaatcaag agaacaactg attcaatcta
    22621 accgatttag ccgtcctatc gttacagaga ttcgccaagc aaagactttt tggccagctg
    22681 aaaatcatca tcaacaattt tatcagaagc aacctaaaag atataaaaag atcaagcatt
    22741 cgcatcagct tcatttattt tttcagcgac atagatagag gaaggttgtg gcgcaaagcg
    22801 ttcagctccg acaaaataat gagcaactga caaaaatagc ttttcatatt tttggacagt
    22861 tatgaattat ttcgaggagt tgctttgaga acaccgagga aaaggttgtg gcgcaaagca
    22921 ttcagcttca agtcgcaagg aaacaggagg tatgacaagt cttttgtcac aactcctgtt
    22981 tttttactag cctttatttc catgtgaaag agaattcgaa ttgtagaatc ttgatttttc
    23041 tatggaattt tgtagagaat catcttgata attaatagac acctaatcga attccacatt
    23101 aatgatgaac taaattccgt ataaaagcgc aaacaataac ta
//
LOCUS       DACEKQ010000047        23038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEKQ010000047 DACEKQ010000000
VERSION     DACEKQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.050"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IW671_001593"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="IW671_001593"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0898580.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IW671_001594"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="IW671_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0898581.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IW671_001595"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="IW671_001595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0898582.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="IW671_001596"
     CDS             4798..5496
                     /locus_tag="IW671_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0898583.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="IW671_001597"
     CDS             complement(5664..8303)
                     /locus_tag="IW671_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0898584.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IW671_001598"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="IW671_001598"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0898585.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="IW671_001599"
     CDS             10146..11321
                     /locus_tag="IW671_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0898586.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="IW671_001600"
     CDS             11325..11966
                     /locus_tag="IW671_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898587.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="IW671_001601"
     CDS             11963..12880
                     /locus_tag="IW671_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0898588.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="IW671_001602"
     CDS             12877..13548
                     /locus_tag="IW671_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898589.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="IW671_001603"
     CDS             complement(14089..14577)
                     /locus_tag="IW671_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0898590.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="IW671_001604"
     CDS             complement(14778..15044)
                     /locus_tag="IW671_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0898591.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="IW671_001605"
     CDS             complement(15044..15307)
                     /locus_tag="IW671_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0898592.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(15300..15572)
                     /locus_tag="IW671_001606"
     CDS             complement(15300..15572)
                     /locus_tag="IW671_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898593.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            15783..15968
                     /locus_tag="IW671_001607"
     CDS             15783..15968
                     /locus_tag="IW671_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0898594.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="IW671_001608"
     CDS             complement(16110..17933)
                     /locus_tag="IW671_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0898595.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..21236)
                     /locus_tag="IW671_001609"
     CDS             complement(18531..21236)
                     /locus_tag="IW671_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0898596.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21542..22489)
                     /locus_tag="IW671_001610"
     CDS             complement(21542..22489)
                     /locus_tag="IW671_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0898597.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22679..22948
                     /gene="rpsN"
                     /locus_tag="IW671_001611"
     CDS             22679..22948
                     /gene="rpsN"
                     /locus_tag="IW671_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0898598.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         6482 a         4871 c         4301 g         7384 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    20041 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    20101 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    20161 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    20221 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    20281 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    20341 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    20401 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    20461 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    20521 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    20581 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    20641 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    20701 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    20761 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    20821 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    20881 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    20941 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    21001 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    21061 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    21121 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    21181 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    21241 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    21301 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    21361 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    21421 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    21481 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    21541 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    21601 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    21661 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    21721 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    21781 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    21841 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    21901 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    21961 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    22021 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    22081 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    22141 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    22201 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    22261 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    22321 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    22381 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    22441 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    22501 ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt
    22561 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    22621 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    22681 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    22741 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    22801 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    22861 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    22921 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    22981 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaat
//
LOCUS       DACEKQ010000048        22882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEKQ010000048 DACEKQ010000000
VERSION     DACEKQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.051"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            311..1090
                     /locus_tag="IW671_001612"
     CDS             311..1090
                     /locus_tag="IW671_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898599.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1136..2080
                     /locus_tag="IW671_001613"
     CDS             1136..2080
                     /locus_tag="IW671_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0898600.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2096..2875
                     /locus_tag="IW671_001614"
     CDS             2096..2875
                     /locus_tag="IW671_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0898601.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2887..3585
                     /locus_tag="IW671_001615"
     CDS             2887..3585
                     /locus_tag="IW671_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0898602.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3613..4377
                     /locus_tag="IW671_001616"
     CDS             3613..4377
                     /locus_tag="IW671_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0898603.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4422..5111
                     /locus_tag="IW671_001617"
     CDS             4422..5111
                     /locus_tag="IW671_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0898604.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            6338..7018
                     /locus_tag="IW671_001618"
     CDS             6338..7018
                     /locus_tag="IW671_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0898605.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            7047..8360
                     /locus_tag="IW671_001619"
     CDS             7047..8360
                     /locus_tag="IW671_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0898606.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            9166..9933
                     /locus_tag="IW671_001620"
     CDS             9166..9933
                     /locus_tag="IW671_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0898607.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            9960..10961
                     /locus_tag="IW671_001621"
     CDS             9960..10961
                     /locus_tag="IW671_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0898608.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            10984..12270
                     /locus_tag="IW671_001622"
     CDS             10984..12270
                     /locus_tag="IW671_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ0898609.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            12304..12957
                     /locus_tag="IW671_001623"
     CDS             12304..12957
                     /locus_tag="IW671_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0898610.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            12990..14195
                     /locus_tag="IW671_001624"
     CDS             12990..14195
                     /locus_tag="IW671_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0898611.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            14325..15650
                     /locus_tag="IW671_001625"
     CDS             14325..15650
                     /locus_tag="IW671_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898612.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            15667..16797
                     /locus_tag="IW671_001626"
     CDS             15667..16797
                     /locus_tag="IW671_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898613.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            16823..17908
                     /locus_tag="IW671_001627"
     CDS             16823..17908
                     /locus_tag="IW671_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0898614.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            17931..18746
                     /locus_tag="IW671_001628"
     CDS             17931..18746
                     /locus_tag="IW671_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898615.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            18730..20448
                     /locus_tag="IW671_001629"
     CDS             18730..20448
                     /locus_tag="IW671_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAQ0898616.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            20474..21310
                     /locus_tag="IW671_001630"
     CDS             20474..21310
                     /locus_tag="IW671_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0898617.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            21315..22748
                     /locus_tag="IW671_001631"
     CDS             21315..22748
                     /locus_tag="IW671_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0898618.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
BASE COUNT         7620 a         3324 c         4928 g         7010 t
ORIGIN      
        1 tattattaat tcaatgttaa tttttttgtt attttatttt ttgtgtaact tttaaaaaca
       61 cctatgaaaa ttaaggaaat aaaaatgaaa tcgattaatt gttatcttat taatgtttta
      121 tttactttta aatcataaat tgccctagtc aagaaaacag tttgtggtat gattatatag
      181 ggaaaacttg tttacaaata tttctgtgaa tatattagtt taattatgtt ttttaaaata
      241 aaaagctgtc atttttatgt aagtgaatta tatcttaatt tatctatcat caatacttag
      301 gagtgaatct atggaaaagc cattcgtcaa gtcattgact gctgtggcgg taggtgcagg
      361 tgctattgcg atctgtttgt ttggttacca tatcaataat cagcggcaac atcagcaacg
      421 gatcaattat gcagaatctg ccatcaccaa tcaaaaagac accttgactt ctttatcaaa
      481 agaagtagac aagctgtata gtacaaaaga aaaaattttt ctaaaccctg aaataacaga
      541 agaaacaatc acaagtttgt caaataagct atcctatgtc aaactttcag cagatgatta
      601 cgacatcaaa gaatcagaat tgcctaaaga agctgttgca atccaagatg agaagaaagc
      661 aatcgtgaca cagttagata gtgcagaatc aaaactgaag atccagacag ctgtcaacga
      721 tttgtttact aaaaatgtat caaactggca gcaagctgta gacgatgtca tcatcaaaga
      781 aaaggtgaca agtgcagatg tcgctcgtgt acgtgaaaat atgagctttt ttaaagatag
      841 tccttggaaa acggttgtta tgcaatattt aggattcgct gatactcaga tcgcgcaagt
      901 cacgcaacta gatcagttat tcgatacgat gttgaaggat ggtcaagtga ctgccaccgc
      961 tacttacgat caatatttga cggcgctcag tcaaatagag cagatccgga atgaaaaaat
     1021 aagcgcagca tacgcaacaa aagcacaaac tgttgctcag caaatgggct atagcaatat
     1081 aagttattga cagttaaaac gttgattcaa gcaaggttag caaacggagg aaactatggc
     1141 aagacgaaga agaaaatcaa gagggaaaaa aatatttctg attattttag cagttcttat
     1201 cgtattagta ttagcaattg taggagtagg agcaaaatta tatctggatc tctcacattc
     1261 tattgagaaa acttacgaat cagtggaacg aacccaagga gaacgaacga aaccagttga
     1321 tctggacaaa caagaatctt tctctgttct attgatgggg attgatactg gagatttagg
     1381 tcgggtcgat caaggaagat cagatacgat gatggttgcc atagtcagtc ctgaagacaa
     1441 acagacaacg atcgtcagca ttcctcgtga tacgtatgtc gacatcgtag gtcgtgacac
     1501 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
     1561 acaaaaatat gtagacatcc caatcgatca ctatgtctct atcaatatga aagggctaaa
     1621 ggaacttgtt gatgcagttg gtggtatcga agtcaataat gacctcactt tctcacaaga
     1681 tggctatgac tttacaatcg gtaaaatcac attgaacggg gatcaggcat tatcttattc
     1741 tcgtatgcgt tatgaagatc caaatggtga ctatggtaga caagagcgtc aaagaaagat
     1801 cgtagaaggt gtagcaaaaa aagtattgag tttaaacggt gtcagcaact atcaatccgt
     1861 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaaaat
     1921 cgctttagat tatcgggagg catttggtac gatcaagcaa gatcagctgc aaggagaagg
     1981 ctttatgcaa gataatatct cctatcaaaa agtcagtgat caagaacttg atcgtgtaaa
     2041 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
     2101 agagacaatc aacttggaag aaatttttga aacattacgc aaacacttgg taacaatcat
     2161 catctcaatg tttgctggct tggccatctc aggaatcgta acatttttca tcttgacacc
     2221 aaaatacagt tcacaagcac aattgatcgt gacactgcca caatcagaca cagcaaatgt
     2281 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaagatatga tcgtcagtga
     2341 tttagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtga aaatgactgc
     2401 tggacaaatc aaagatgcga tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
     2461 ccaggcaata agcacgaatg cagtgactgc tcaacagatc gctaacacaa cttcacaagt
     2521 tttccaagag aacgcaaaag acgtgatgaa tgtcgataaa atctcgatta tttcaggtgc
     2581 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
     2641 attaggaatg atggtaggcg taggtctggc attcttattc gaattacttg atcggacagt
     2701 gaaagatgaa aaatatgtga cggatgcatt aggattccca atcttaggaa cagttccaga
     2761 aatgtcagcg aaagaattga acgctggtat ccgtaaaaaa ggaacagctc ctgcacgacc
     2821 aaaagctaca aacgataaaa cagccccatc acgcagaagc cgctcaagag tctaggagga
     2881 agaaaaatgg cacgaacaca gaaacaaaaa gcagcacaaa ctcatgcagt cagtttgatc
     2941 acattaacaa acccatcttc accaatcgcc gaacaatatc gtacgatccg gacgaatatc
     3001 cagtttgcct ccgcagctgg tcagcaaatc aaaacgattg tcgtgacttc atcaggtcct
     3061 ggagaaggaa aatccaccac tgcagcaaac attgcagtcg tttttgctaa atccggtcaa
     3121 cgagttctac tagtcgatgc agatttacgt aaacccgtcg tctataagac attccagctg
     3181 aacaatgcca ggggattaag tacagcactt agttcatcag gaagtgtagc agatgtaatc
     3241 caacgaacgc cagtcgagaa cttgtcgatt ttgcctagtg gacctaagcc acccaaccca
     3301 tcagaattgt taagttcgcc aagaatggat cagatcctag acgaagcaag acagttattt
     3361 gacgtggtga tattcgatat gccaccagta gtagccgtaa cagatgcaca gatcatgtct
     3421 tcaaaaacgg atggcacttt attggtcgta cgtgaaaata catcacgtaa agaatcattg
     3481 aataaagcaa aagaactgct agagatggta caagcacgag ttttaggtgt tgtatacaac
     3541 ggagcagaac atagcaaaga tgctggttat tactacggaa attaaggaat aagaagagag
     3601 gagacaagag aaatgatcga tctacattgc catatcctac caggaatcga tgacggggca
     3661 gaaaacttag aagcaagtat tgcaatggct gaaaaagcga ttcagcaagg catcacgcat
     3721 attttatgca caccacacca caataatgga aaatacagca atgaaaaatc acaagtcatt
     3781 tctctagtcg cttcgttaca agccgaatta gagaaaagac agttgccttt gacgttattg
     3841 gaaggacaag aagtacgtat taccggaacg ctcatcgaag acatccaccg agatgaaatt
     3901 ttatttacag atttagatga tacatatctt ctgatcgaat ttccgacact ggaagtgccg
     3961 ttatatgcag aatggctttt ttttgagttg ttagaactag gaaagacacc agtgatcgtc
     4021 catccagaac gcaacgcgca ttttcgagaa gacccaaatc atttgcttcc gttcctagat
     4081 atgggatgtt tggcccaaat tactgcgcca agttatgttg gtgtatttgg caaagacatt
     4141 cagaaaacag ccaaaacaat ggtcaaacat aatctcgtgc agatggctgc ttctgatgcc
     4201 catggggtct ctaagcgtac cttttactta aaagagtgct atgagcagat tgctaaggac
     4261 tttggtaagg aaaaagtagt acagatgaaa caagtcgcaa aggatctgat caacggggat
     4321 gaaatcagtt atccagtcta tgaagaggta aaaaagaaga agtttggttt attttagttc
     4381 tttattcgta gataaaaagg aaatgaggag agagggaaag tatggaaagt gaagatttat
     4441 tatctcgtat tagtgaagta catcctaatc aagtttggtt ggataaagaa aaagttgaaa
     4501 aacgatatat ctatttattt tttaaaagaa tgattgatat tgtaggaagc attattggtt
     4561 tgattttagc ttctcctttg atgttctatg tagcttatcg aatcagaaaa gaagagccag
     4621 gaagtccgat atttttttgc catacacgtg ttggtaaaaa tggaaaaaca ttcaaaatgt
     4681 ataaattccg ttcgatgtgt ttagatgctg aagaaaaatt ggaagaatta cttgatcaaa
     4741 atgaaataga aggtgcaatg ttcaagatga aagaagatcc ccgtgtcact aaaataggta
     4801 aaattatccg tgccaaaagc attgatgaat tgccacagct atggaatgtt ttaaaaggcg
     4861 atatgagtct aattggtcca cggccacctt tggagaggga agtagctgag tacacgagat
     4921 atgatcttca acgcttgtat gtaaaacctg gttgttcagg tttgtggcaa gttagtgata
     4981 gaaataatgt tagtttccat aaaatggttg agttggattt agagtatatt agtcatcgaa
     5041 gtttaatgta cgacttgagg ttgattttaa aaacgtttta tgttattgtc aagcccaatg
     5101 gagcatatta gttagggata gaatgatgga ttgttaataa aaaagattac acatgttcat
     5161 attatcgttt caaaagtaat aactgtaatt atggagggtt tgaatcactt gtagaaaatc
     5221 taaaaaaaga aaggtgaatg gggcaattca gtatcattat tcttatataa aagaaaatat
     5281 tcgaaatttt gggatgatac cactcttaaa tataatgatc taaatgcttt aatattggta
     5341 tatccgatat aggaactgac gagctattta gtatgataca aaagtatatt gatagccatt
     5401 aataaatttt ccaaaaaata atgacagcac accaattttt ataatttagc ttgttgaatc
     5461 ggtatgttca tgggacggtt caaaaaaaga atcgaaaaaa taaacggtaa aattttagtg
     5521 aatcctatgt aataaaattt ggatagatgg aaaaacagca cataaggagg ctgagtttgt
     5581 tgtcatttct gctcctacaa actatggtag tgaatgtgtt attgaattag tgatgtctgt
     5641 tacttcagag acattgggga taattaactt cactatacct gtagggtata caaaaaatgg
     5701 aagaaaaaat attatcaata gattttattt agttcagaat ttttacgtgg aagtaaatta
     5761 ttgatgataa tctttatcca attagaatta ttatcgtagg atgtgatgat gatagccgtg
     5821 aagttgttca agtatttacc aatgtctaca acgcagaaaa ttagtttggg tttttcggat
     5881 tggaaaattt caatgtataa aatcataaat ttgctgtaaa tagacagttt ggggtagtaa
     5941 tagcccaata aattgttcga taaactcatc tttggggtga gaatagctca atcattgaat
     6001 ttcaaacctg cctttggggt gggattagag gtcaatatgt acctacataa tactttttgg
     6061 aaaatgatgg tgttgagaat gtaagtacat ttcaatgtga aattctaata tagccccaaa
     6121 cgaggtcaat aaaaatgacc taaactcgta tttctcccct aaacgaggag gagatagata
     6181 tgaaaattga atgtgccggc gttggagttg gctgctttta gtaggaatga acatggttcg
     6241 atgagaactg tttcagatag agtggtcgat gatcacaggc ctttaagtcg tttacagatg
     6301 atgagacttg taaaaataga gggacgaatg gaaaaagatg attggtatag aagaaaagaa
     6361 aggtgttcag aaagacgtaa caattgatag ggaagagaat cttaggggac atagattcta
     6421 ttggattgga cgcagagcac aagatatctt cttctcggct ctagctttga ttgcattgag
     6481 ccctgtgatg ctaattacag cgctcgccat tgtgattgac gatcccggtg gcagcccgat
     6541 ttttgtgcag gatagagttg gaagaaatgg aaagctgttc aagttttata aattccgatc
     6601 tatgtgtgta gatgctgaag caaaattaga agccttgttg gcacaaaacg agatggatgg
     6661 tccggtcttc aagattaaag atgacccaag aatcacgaga gttggtaaat ttattcgaaa
     6721 gacaagtatc gatgagttac ctcagctctg gaacattctt aaaggtgata tgagtattat
     6781 tggacccaga cctgctttac cgagagaggt ggaacaatac ggtgactacg aaaggcagcg
     6841 gttgttcgta cagcctggtc ttagttgcta ttggcagatt gctccacatc gtaatgaact
     6901 gtcctttgaa gaatgggtgg aacttgatgt gaagtacatc aatgaaagaa gctttatcac
     6961 ggactggaag attatcttct tgactttaca ggctgtatta tttggatatg gtgaatgatt
     7021 atatatagta ttaggagttg aaatatgtga atattatttt attatcgggt ggtagcggta
     7081 ggcggttatg gccgctgtcc aacgatattc gtagcaaaca attcatcaaa ctgtttaagg
     7141 atgaaaatgg cgaatatgaa tctatgattc aaagagttta tcgccagatt acaactgttg
     7201 acccagatgc aaaggtcact attgcaacaa gtaagtctca ggctagtgca atccataatc
     7261 agttgggaga taaggcttcg atttgtgtag aaccttgtcg tagagatact ttccctgcaa
     7321 ttttgttagc tgcagcatat cttcacgatg aacttggcgt aggtgatgat gaaagtgttg
     7381 cggtttgtcc tgttgatcct tatgtagata attcctatta cgaaggcgtt gaagcccttg
     7441 agaagttggt gcagcagaat acggctaatt tgactttgat gggtattgca cctacatatc
     7501 ctagtgaaaa gtacggttat atcatccctg aaagttctaa cgaagtaagt actgttaagg
     7561 agtttaagga aaaacctgac aaagcaacgg cagagaaata tttagctcag aatgctgttt
     7621 ggaatgccgg tgtatttgcg ttcaaacttg gttacctgtt ggaaaaagct cacgcaatgg
     7681 tggattttga agattatcgt gatttgtatt ccaagtatga gactttaacc aagatcagct
     7741 ttgactatgc tgtggtcgaa aaagaaacga gcattcaagt gatgcgttat aacggggatt
     7801 ggaaagatgt cggaacttgg aacatgatgg ctgaagttat ggctgataat attaagggga
     7861 aagctgtaat ggatgatacc tgtgttaata ctcagattgt gaatgagttg aatattccta
     7921 ttcttgctat gggctgcaaa gatatggtaa ttgcagcaag tggcgatgga attctgattt
     7981 ctgataaaga gcgcagtggc tatatgaagc cgtatgttga gaaaatcagt acggaagtaa
     8041 tgtttgcaga aaaatcctgg ggtacatata ccgttatgga cgttcaggaa ggctccatga
     8101 ctgttaaagt ttctatgcag gccggacaga agttgagcta ccactaccac aattctcgcg
     8161 atgaagtttg gactgttgta gcaggacagg gtaaagcgat tgtagatggt atgaagcaga
     8221 ttcttcgtgc gggtgatgtg gtgactattg ccgcaggatg taaacataca atcgaagccg
     8281 tgacaaatct ggatattatc gaagttcaga ttggtgaaac tattagcgtc gctgacaagt
     8341 ataagagttc tttgaactaa aagttcgcga ggtagttatt atggagatac taaaatttca
     8401 acccgtgggt aaagattatc tttggggtgg agaacggttt aagacggaat ttggaaaaaa
     8461 ttgacttaac accattaagc ttatacgtgg gaatgcttag ttcatctaga cgataactac
     8521 gtacggaaat atcaaatatg aaggacagaa tggtaaaacc gagttgtggt atgtattgga
     8581 agctacaccg gggggtgatt tatgcttttg agtacgatgt aacagaagaa atgttacgaa
     8641 atgctgtgat ggacagatca cttgggaaac atttatataa agaagaagta cacaagggag
     8701 atctttttta tgtcagttca tggtattggc gctggagtat tagtggctga aatacaggaa
     8761 agctcaaatg ttacctatcg agtttacgat tatgatcgag tagataagaa tgataataag
     8821 tgagaattgt actttgaaaa agcgattcag gtaatgaata tggaagccac tttgaaattg
     8881 gagaaaagaa ataagctcat tcatttttat cctggtggct ctcgtgaaat actgtgtaga
     8941 tgtaaatact gtgagcccga aagaatccaa gtaaagacgg ggttttcgtt ctcggtaaaa
     9001 gaggaatctt ttcaagttct tttatgtata tcgggggacg gacaagttga gtcggcaaat
     9061 ggtgttatga aactcattag atttgtaaaa aagatattgt atgtttttgc caacgaatat
     9121 tggaagatgt taagtgttga gcgatgcgga cttactgaaa attagatggg gatagaggga
     9181 ggatatctaa tgtcacgaat gagatttatg aacacagaaa ttgacaatct tacaatgggt
     9241 gaagtattgg tagaggttga aaaaatgatt caagatagga gatgcggata tgttgtaact
     9301 ccgaatgtgg atcacattgt tcagttggaa acaggaaaag agttgcaaga tgtttataaa
     9361 aacgcatctt taattttgac ggatggtaaa ccattgattt ggatttcaaa gttttataag
     9421 acaccaataa aagaaaaaat atcgggatcc gatatatttt ctttactgtg caagatggct
     9481 gcagaaaagg gatacacaat gtatttttta ggtgcggcag agggcgttgc ggctaaagcg
     9541 gcagataacc tgatgaaaag atttccggga ttaaatgttg taggaacata ctctcctcct
     9601 tatgggttcg aaaaagacga agatgaaatg tttaaaattg attcccaaat taaacgagca
     9661 aatccagata tgcttattgt gggtttgggt tgccctaaac aggaaaaatt tatgttttat
     9721 aactgtgata gattgggagt acctgtgtct tttggtctag gagctagttt ggactttgag
     9781 gcagggaatg ttaaaagagc accaaaatgg atgtcagatt atggatttga gtggctgttt
     9841 agaattactc aggaccctaa aagaatggca aaacgatatt tggttgatga tttaaaaatc
     9901 tttaaattag cactaaaata tcggaaaaaa taaactatat tcaatgagga caatgaagta
     9961 tgattattac aaaaacacct tttcgcatgt ctttttttgg cggcggaacg gatatggaag
    10021 attattttcg agaaaatggc ggtgcagtgc tttccactac ttttgataag tactgttatg
    10081 tgaatgtgcg tcatttgcct agattttttg attacagcac agagttatca tattctcgta
    10141 ctgagagagt aacaaatatt gaagatatcg agcatcctgc aattcgtaat gcgatgaaaa
    10201 tgctagatat gcatgaaatt cgccttacat atgaagcaga tcttcctgca cgttctggtc
    10261 tgggtacatc tagttctttt gccgtaggta tgttgaatgc tttctatgct ttgaagggca
    10321 agtacgctga caagaagaaa ctcgttgata aagcaattta tcttgaaaga gtcctttgta
    10381 atgaagcagg cggatggcaa gatcagattg cagcagctta tggtggcttt aaccgaatca
    10441 atttcaactc agatggctat gaagtattgc ctattattat tacgcctgag cgtaaaaagc
    10501 agcttaacaa caacttgatg atgttcttta ctggattcac cagattttcc tctgatgttc
    10561 agaaggcaaa taatgtcagc ggcacagagg acaaaagagt aagactgaaa aaaatgtatg
    10621 agcttgtgga tgaagcagaa gcggttctga ctgataagaa cagagatcta gatgatttcg
    10681 gcagactgct tgatctcact tggagattga aaaaggctac cggtggtgcg attagcactg
    10741 gaagtatcga tgaactttat gaaaagggca tggctgctgg tgcattgggc ggtaaacttc
    10801 tcggagcagg tggcggcggt ttcttggttt tttatgttca gcctgaaaaa caaaatgctg
    10861 tgcgttcggc aatgagtaat ttgatgtata ttccgtttga atttgaaaac ggtggaacac
    10921 aggttattca ttacacaccg gaaatgtata ttccaaaata aagaaatcag cgaggataag
    10981 aatatgaaag ttgttattat ggcaggcggt aagggaactc gcatctcatc ggttgcaagc
    11041 gatattccaa agcccatgat taaaattgaa aacaaaccgg tattggagca cgaaatcgaa
    11101 tgtctgagag accagggatt cacagatatt attcttaccg ttagccatct tggcgatatc
    11161 attatgaact attttggcga tggttctgga atttcaccag ttactggtca gccctttgac
    11221 gttaaaattg aatacttttt ggagaaagag ccattaggaa atgccggtgc attgtttcgt
    11281 ttaaaggata atttgaccga agacttcctc ctgctgaatg cagatgcgat gtttgacgtt
    11341 gatttcaacc gatttgtagc atatcaccgt gaaaagggtg gcttggtgac attgtttact
    11401 caccctaaca gtcatccata tgacagcggt ctaatcattg cagataagga tggctctgta
    11461 gacagatggc ttgtaaagga agattcccgt ccaacttatt accgtaacag agtaaatgcg
    11521 ggactccatg tgattagtcc tgctgttctt gatgtcgaga taaacacacc taagattgac
    11581 ttggaccgtc agttattaaa gccgttggca ggaaccggaa aaatgttctg ctatgatagt
    11641 ccagaatacg taaaagacat gggtacccct gatcgttatt atgctgtctg tgaagatttt
    11701 aaggctggaa gagtgaaggg caaaaatctt cagaataagc agaaagctgt tttccttgat
    11761 cgtgatggaa cgattaatca atatgttggc tttctgcgtc atattgatga gttcgaacta
    11821 atcaagggta ctgaagatgc agtgaaaaag atcaatgccg ctggatatct ggctattgta
    11881 ataacgaatc agccagtaat tgcaagaggt gaagtttctt ttgaagaatt ggaagagatc
    11941 cataacaaaa tggagacact tcttggatat aagggcgctt atttggatgc agtttattat
    12001 tgcccgcatc atccacataa gggatatgag gaagagcgcc cagagctaaa attcgattgt
    12061 gaatgcagaa agccaaagcc cggaatgtta attcaggcag ctacggactt caacatagat
    12121 ttgagtcagt cttggatgat tggcgatggc gagaatgata ttaaagcggg ccaagccgcc
    12181 ggctgtaaga ctgcattggt tggcgagagt gagtatggac aggatatgac catagcaacc
    12241 ctgagtgaat ttgcagataa gatctgctaa gggatgtttt atcacgaaag gagagtataa
    12301 acaatgatag acaatagatt acagaaacac attgatttgc tgattaaacg ctatcccact
    12361 cttgaagtaa tcaagcagga tattgttgac ggatacctga ttatggagga atgctattta
    12421 aatgatggca agctgctgat agcaggaaat ggcggatctg ctgctgattc cgagcatatt
    12481 gcgggtgagc tgatgaaaag ctttaagacg cctcgccctg ttagtaggga atttgcagat
    12541 aaattgattg cggttgatcc tgtaaagggt tctgaattag ctaagaatct tgaatatggt
    12601 ttgatggcga ttcctctcgt tgctcatgaa gcgctttcca ctgcgtatat taatgatgtt
    12661 gatggcctag gtgttttcgc acagcagttg tttggatttg gaagacctgg cgatgtattc
    12721 cttggtattt cgacatccgg caatagcaaa aatgtgataa atgcaactgt tgttgctaga
    12781 gctcttggta tgaaagtaat tggcttaact ggagccaaag gtggagaact tgcaagggta
    12841 gcagatgttt gtgttaaagc tcctgaaaca gaaacatata tgattcagga gtaccatttg
    12901 ccaatttacc actgttggtg tctaatgttg gaagataaat tttttgggaa tagataaaat
    12961 atctaagccc atattgaaag gataaaatta tgaatgtact atttataaca actcaatttc
    13021 catatcctct agataatggc ggaaaaattg gtgcgtacaa cggtatcagt gttttatcta
    13081 gagactacga tgtgacggta ttgtctttca ccgaagaacc gcagtatgtt gaagaaggtg
    13141 tcagtttttt tcgagagaaa tttaatagtg taaaatttga aaatcctgtg atacatgatg
    13201 tacacataag aaataatcca atgaaattgg tagaagcttt agcaataggg tattgtctac
    13261 gccaacctta tatagtttca aagttttatg attcaaaaat gctgcagtta atagatgaaa
    13321 aattttctaa caatgcatat tgggatatca tttttattga ctatctgaat atgcaaagtt
    13381 atggtcaata catacgggag aagtataaaa cacaatttgg taagtatatc ttaaaagacc
    13441 acaacataga atatgattta gtcctacagg aagctgaatc aagtcgaggt ttaaaaaaga
    13501 agttgcttca gttagagtat aagcgtactc gaaagtatga agaggccgca gtaaataggg
    13561 ccgattattg ctttagtgtg tgtgatgcaa atacggaagt actgaaaaag attaatgaca
    13621 acagttatac gatgcttcca acttatgaaa tgcttacagg ggtgaaaaaa aatatagaga
    13681 agaacacgat cctatatttg ggtaaccttt cttggaaatc caatatgcaa ggattagttt
    13741 ggtttttaga gtcaatttat ccaaatattc aacaaagaat tccggatgta aaaatcacaa
    13801 ttgtcggtag cgggccagtt ggaacagatt ttagtaaata ttcaggcgtg gattatcgtg
    13861 ggtatgtaaa agatatttca cacatttatg acgatcaagc ggtgctaatt gtacctttgt
    13921 tagaaggtag tggtataaga ataaaaatat tagaagcgtt caataatgaa attgcagttg
    13981 tatctacatt agttgggtgt gatacgattg gcgcaaaaga tggaagtgag ttatttatag
    14041 cagataacga aaaagatttt gcaacggctg tagttagact gcttgaagac atgaatttga
    14101 atgaaaaagt aagaaagaat gcaaaattat tcttaaaaga aaaatactcc ttaacggcta
    14161 gacaagatga gtttagtgcg ataattaaca gctaatatat atataagctt atctgatgat
    14221 gatttatagg aggaaatata tggagatggc tgagagaaag gaaaagaaac ttggaaataa
    14281 gaatatcata ttattttaga aaactcctgt tattttatgt ttgtgtgatt gttgcggcta
    14341 tgagttacca ccttacaggc aattccaatg tagcgtcatt aattgctgta gtaggagtag
    14401 tttatgctat tgtaaaggcg tgtaataacg agtatctggc tatatgggtt atgtttgtat
    14461 taggttctga tatatggggg atagtgccat atattaacat tggtagtggt gtgttgcatt
    14521 ggtcagattt taatgttata gtagcgatat ttctctgctt gagattcatc aaagttaaag
    14581 ctaggagtag atacgggatt gtctatagtg taatgcttat atttattttg attgcatgtg
    14641 tgcaatcgca ttcaatctat ggacaagatg tacatacaac gctcttaaca gctagagaat
    14701 tgctagttgt aatatccttt tggccgatag taaaaatagt atattcagaa agaataacta
    14761 aaaatgaatt cattgattta gtgtgtaaat tcacaagatg gacaatgtgt atatattggt
    14821 ttgaatggat tcttgttaat atgggattca atataacctt tttacagaca ggtgcacgct
    14881 ggggtactag aatctatata gatccgatat ttatcatttt atatttcttt ataaatctat
    14941 atgcggttat atataaaaga gagggctata atagtacaaa taaaagtata tggaattgca
    15001 tttttagttt actgacattt gttataatct cgcaaggcag aagtggtata ttatatgtag
    15061 taataacctc ggttattatg cttttaattt cgcttaaacc atccaagatt attagatttg
    15121 gtatattagg aattgcctta attgcatgtc tagtgtctgt gacccaaatt agagaggtcg
    15181 ttatatcttc gtttgtagag tctcagagca gtgacacagg taatatcgca tatagaattg
    15241 ctgaacaaga ttattttgaa aataaattag aaggtcatga actatttgga gttggtatac
    15301 caaacaatca tgatgccaga gctgtagaat attcaggcaa aacaatagat tttgagtcta
    15361 aaaaatataa tccctattat cttgaggact taggcgcttt tgaaattcgt tatagatttg
    15421 gtataattgc atatgcgtta tatatagttg ctttagctat aagtattata gatgccggat
    15481 ttcgaagaga caaatcggtc gccgcatttg cgggtatggc attcatggtg tttatggcgc
    15541 ttaattttaa tttgctagcg tatattacag caagaccgtt cggatttatg atttctttaa
    15601 ttttgataga aatatcggca ttcaatgatg aattcatata tattgaatag gtaagggaga
    15661 gcacagatga atttagatcg attaaagaaa atagcaaata tgatttcacc aatcataaaa
    15721 gtgataaatg cgccgttagc gtattttagt gggaaatcag attatatgga gaagggaata
    15781 ttgtggccta ctgtaacaac ctactccaat aacagtagaa aagtgattca atatacaact
    15841 ttacctaaaa acgtagacaa catggctcaa atgcaatggg ctggggaaag ttttgaggac
    15901 atatgtatag ttcttcaagg tccagtatta gaagacggtg attttacact aaataccgtt
    15961 aaaatgtata ataaatacta tcctaaagta aaacttgtag tgtctacttg gataggatta
    16021 tctgaagaga cagaatgcaa gttgcgtgaa gctggtgcag atgttgtatt atgcaagaaa
    16081 cctgaaaaaa caggtgcgta caatattaat ctacaactta cttctgcata cgaaggcgta
    16141 aaaatggcgc aaaaactggg gtgtagttat gcgctgaaaa cgcgaactga tcaaagacta
    16201 tatgcaaatg atgtactaaa gtatttccaa acattattga atatttttcc atcttcggac
    16261 tgtaaagtag tgaatcagcg attaatattt acgacttatg gaaaatcatt tagatatctt
    16321 ccgtttaatt tatgtgattt tttggtatac ggagaaacta gtgaattatt aaaattatat
    16381 ggtattcaaa gagataaccg agcatcagac tttgggaaaa ttatttatac tgaggaaaaa
    16441 gagtttaata aaaagatatt tacactacta gaaggaaaac aaggtgtatc tccatatgat
    16501 gtttttccag attttgatgc actatataaa aaatatatgt ttgcagaata ctatattgtg
    16561 tataattatt tcctggaaaa tattgcaagt aatgaatctt atggtgactt aatggatgcc
    16621 tattatagtt atttaaagaa atatgcagtt gttgctgatt cagaaaaatt gttactatat
    16681 tggccgaaat atgttcaata taatgttaag ttcgaaaatg aacttgattt ctatgcgaag
    16741 ttggattcaa aaaaatggat ggatatttat gctaattata caccttttag tcagtgatta
    16801 tatggaaagg atgaggtgaa taatgaaaat cggaatagtg actattgtta atggggcaag
    16861 ttttggtaac agattacaaa attatgcgac tcaaaaagtg ttggaaaata acggctatac
    16921 agttgagaca attaataatt gttttgacaa agaaagtacc ctatccattg ttatcaaaaa
    16981 cgctatttat acattattta agtttatacc gcatttcaaa aagtcaaaaa gtatctggaa
    17041 aagaatgtat acattttatc aatgggacaa gcagtatata gattattctc atcgtaaaat
    17101 tgatgcatct aaaaaaaaca gctttgtgag tataaagaat gattatgact atttcttggt
    17161 tggtagcgat caaatttgga atccaaaata tgcagccaat aagggatttg aacttttatc
    17221 atttgttcca aatgaaaaaa gagtttcatt atcaaccagt ttgggagttc catctatacc
    17281 aagtgattta gaaaaattat atgtgacaga gtggaataag tttaaatcaa tatcggtacg
    17341 tgaaaatcaa gcggcaaaaa taattgagga cttgacaggc acaactgcgg ctgttcacat
    17401 tgacccaact ttaatgttgg agcgttcgga atgggaagcc tttcaaaaaa aaccagacaa
    17461 tattgatgaa aattacatct tgtcgttttt cttaactaat ccgcctaagg atactgttga
    17521 tgtattagat aaggtcgcta aagatttgaa aataggtgtt ctcaccttag atgcgtcttg
    17581 ggaatatttt caagatatta ctccgaatca ttttgtttat ttgatttcac atgcaagaat
    17641 gatttataca gattcattcc actgcacagt tttttcattg ctatttggaa aaccggttgt
    17701 ggtatttgac cgttttgata gtgaaaaaat gaattctagg ttaagtacat tgttaaataa
    17761 agtaaataaa gcacattttt gggtaggaaa aaaagctgat gaagcaatga ttcgtgcaat
    17821 agcatctgaa cctgaatatg acatagataa gctgatggta tcagagaggg aatgtgtaaa
    17881 agcttatctt aaagagtatt tacagtaaaa ttttgaaatg ggatgaaaat atggagcatt
    17941 taagtgattg ttttggtaaa atgaagattg ttacgacttc atgcgcaaga gaatatttta
    18001 aggatgattt ggataaatca gagtattcat gttttgttga ttctcgagca gctatgtttt
    18061 tcgcaatggg acaagcttta gctaaaagaa ggaatgtagt tgtaatgctt gctgatgaat
    18121 tgacatcgtg ttatacggca ctaacagaat gtgcatttca acaagttgat ttaattgtgt
    18181 ttgcttttca gacagtcaga catgaaattg atttagcatc tttgaagaat tgtggttgca
    18241 atctaattaa ctgtagcgcc caagacgata ttgatagagt tgtgtccaac actaaggggt
    18301 taaatcagac aacagtaatt aaaatcaata tgaattgtga gtcggataaa gatcatatat
    18361 gtgagtatat agacctgcca gacatacctg tttttttcca cggtagctta tactcacctg
    18421 caagtaaaaa gaaatatgag cagtatggac aggtatcgaa gtatttaggc tatctcgtgg
    18481 ggggaaaaga tgcaatactc ataatttctc acaatgagtt tttaaacgat atcagtgtat
    18541 ggattaatta ttattctttt ggagaaattc aacccaaaat tattatattt gacagtccga
    18601 atgactatca atataaatgg ctgtgtgact tagaatgcag tgtagctcat tcaaccaacc
    18661 gatttttgtc tgaggattta gccttattgc aaaataacgg aatttccatt gtgttaagag
    18721 gtgataatta tgatatcgtc agttaaaaat gttcaaattt tagtatcttt attaaaacag
    18781 cataatatta aagatatagt tatctcgcca ggtagtagag attttcaatt ggtgcatagt
    18841 gtagaaacag acagtttttt tagaacctat acggttgtag atgagcgcag tgctgccttt
    18901 tttgctaatg gactttctat ggcgattgat aagcccgtgt gtgtatgttg tacttcttca
    18961 actgcctctt caaattatat ggcggcagct aaagatgctt ttgctaatgg catccaactt
    19021 gtacttctta ctgctgatag agattaccgc cgcttatatc agatggaaga tcagatgatt
    19081 gatcaggtag atatgtatgg ggcttatgtg cgttctaacg ttgatattcc tattgttaag
    19141 gattctgatg atgaatggta tgctaccaga aaaataaatg aagcaatatt ggagttaaat
    19201 catggcgtta agggtcccgt gcagattaat tatcaattaa tggatttaag tgcgccgttt
    19261 gttgagacca tgccagagta tcgtaaaatt acaagatatc aagaggatga tttactgtca
    19321 tcagtagaga tttttaaaca gaaactatta tcaaaaaaga gaataatggt tttatgtgga
    19381 cagcaggaag aaaataattt aagtgcctta ttggaagagt tctttattaa atataataca
    19441 attataatca aggataacta ttctaatata cacggtgaac actttattaa gactgttcta
    19501 gtaacagaaa gaatgagtgt aaatgcattt attgattatt gcccagattt ggtaataact
    19561 attggtaaac atgtatggtc ttttgtgaaa tatactctta gagataaaaa gcttaaattt
    19621 gagcattggc ggatatctga ggatggtaag attgttgatc cgttcaacaa gctaacatct
    19681 gtctttcagt gctcagcgga attttttatg gagactgtca ctgctggtgt gaatagcaat
    19741 aataatcttg agtattataa tctatggaag acaagattat caaaagtttg ctatccagag
    19801 ttagtctata gcaattttta tgcaatttca gaactagtaa aaaaaattcc agatagtgca
    19861 ttagttcatt ctagtatttt aaatgcggct agattgtatg actttgtatc tgatgataaa
    19921 gcattactca gttttgctaa tttaggggct gatggtattg atggttgttt accaacattt
    19981 ttaggtcagg cagaagcaga taaaactcga cctgcgtatt taatttcagg agacttgagt
    20041 ttcttttatg ctatgaatag tttatgtggt gacattccag ataatgcaca tattttcctt
    20101 attaacaatc attctggaag tgaattccat acaaattttg gtaagtttta caacggatca
    20161 ttaagtgttg ataagcatat agctgcagga catatttcaa acgcaaagga ttgggcatta
    20221 gaaaataata taaaatacat tactgcggat tctaaggaaa gtctcaaatc ggggttggat
    20281 gtatttatta atacatttga cagtcctgtt ttatttgagg tctttacaga tccggatacg
    20341 gatacgtctg ttctggaatc tttttatagt ataaataagg taatcacacc aaaaatgtat
    20401 tttagaaagg cgttagatat tataaaaaag catttacaca ttaagtagat gtttattgcg
    20461 aggtgaaaca aatatggcaa taaaatctta tattaaacgg ttattgcttt atatccgtca
    20521 tggagatccc aaaataaatt tagatgtgag ttatttatct gaaggtgcaa tgcttgctgg
    20581 taagactgtg ataattacgg gtggtgcaaa aggacttggt tttgcaattg caaaatcatt
    20641 tatagctcaa ggcgcaagcg ttattattac aggaagagac atggattcgt tgaataatgc
    20701 taaatgtcag cttggagata tgtgttctat cagggcgttt gatataactg atgctaaaat
    20761 gtatcgaacg ttttttgaag atgcatacga gacctttgga cgtattgata tactgcttaa
    20821 taatgctggg atctcgttac acgaagatgg atttatgagt gttactgaga aaacttggga
    20881 taagcaaatg gatacaaatt tgaaaggtgt ttatttcttt acacaggaat ttattaagtt
    20941 tgttaaagaa aagaatcaaa tgcaagcaaa tattattatg atgtcctcag aaagaggctt
    21001 gtgtggtgat agtattccgt atggattatc aaaggctgca attaactctt tagttgtagg
    21061 cttgtctaag aaatatattt ctgagggaat tagggttaat ggtattgctc ctggagtaac
    21121 tgcaaccgct atgactggta tttctgaaaa gggtaatttg tataatcctg caacgatcgg
    21181 ttatagatat tttctcccag aagaagttgc tcaagttgct ttgtttttgg caagtagtct
    21241 ttcaaactgt atcagtggtg aaatcatagc ctgtgaccag gggcaatatt taagtcgaaa
    21301 ttgggggtaa taacttggga atattattaa aaaaatataa tagccttagt gagcctgcaa
    21361 aagcatctat ttggtttaca ctttgtaatg tgttgcaaaa aggaattcta tttttgacta
    21421 cacctatttt tacaagaatg ctttcaactg cagaatatgg taagtatacg atttttatat
    21481 cgtggtattc tattatagca attttcacaa cgttgaattt atctgcaggt gtctataata
    21541 aaggactaat taaatacgag aataaatatg cattaacctc gtcattttta ggattatcgt
    21601 catttatttc cctttgtgca ttgatgctat accttccatt tagaacggtt ataaatgggc
    21661 tactggattt atcgacggag tttgtcatat tgattcttat tgaatcaata tttgagccgg
    21721 cttttttgct ttggtcagtt aaggaacgat ttgattacaa gtatcaaaaa ctagtactca
    21781 tcacagttct tatggctgtt ttaaatccag gtctcggtat tatattaatc ttatcatttg
    21841 gggagtatgc tatttggaga gtacgagctt atgtcgtggt aaatgttgta gtggggatgt
    21901 ttattggcat aggaatactg gctaagggta agaaattatt tgataaagag tactggacat
    21961 atgcgctcag atttaatttg ccattaatac cacattattt atcaatgaca ttgctgaatc
    22021 aggttgatcg ccttatgata aatagtatgg ttggagctag cgaggctgcg atttatagta
    22081 tcgcttacac tatatctagt atgatgaata tcataacaac agctataaac ggttctttta
    22141 ttccttttac atacaaagca attaaagatg gcgtgtatga aaaaattaaa tccgttgcta
    22201 atcaactatt agtgttgatt tttgtcttgt gttcgtttac tatgctgttc gggccagaat
    22261 tgatttgggt tgtcggagga gaaaaatata tggatgctgt ttggattatt cctccagttg
    22321 cggcatcagt atttttcatg tttttatatc cgctttttgg caatattgaa ttttactatg
    22381 aaaagacaaa ctacgtaatg tttgcatctt gtgtgggctc tatcgcaaaa attatttcaa
    22441 actatgttag tattccccgt tttggatata tttcggctgg ttacacgaca ttgatttgct
    22501 atatgttgtt ttcctttgcc cactacatgt tttataaaaa aattcttaga gaaaatggta
    22561 taaaagaaga tatgtataat attaaaaata ttatatttct ttctgtctta atgattgtgt
    22621 taactattgg tatgacaata ttatactttt ctacaattct aagatatagt attattattg
    22681 gattagttat tgtggctttt cttaaaagga cgcaaataat gaaaatcctt aaaaatataa
    22741 aaaattaaca agcaaatctt gggaatggaa cggaggcacg gtgaaatgag attattgatt
    22801 ccatctgcta agattgtacc ggaagaactt caccatcttg gaaaacttcc tgcaattatc
    22861 tatccgatta atcaaaaaat tg
//
LOCUS       DACEKQ010000049        22857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEKQ010000049 DACEKQ010000000
VERSION     DACEKQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.052"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW671_001632"
     CDS             complement(104..1336)
                     /locus_tag="IW671_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0898619.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW671_001633"
     CDS             1591..2160
                     /locus_tag="IW671_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0898620.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW671_001634"
     CDS             complement(2338..2778)
                     /locus_tag="IW671_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0898621.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW671_001635"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW671_001635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0898622.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW671_001636"
     CDS             3732..4655
                     /locus_tag="IW671_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0898623.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IW671_001637"
     CDS             4731..5870
                     /locus_tag="IW671_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0898624.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IW671_001638"
     CDS             5863..6663
                     /locus_tag="IW671_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0898625.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IW671_001639"
     CDS             6663..7490
                     /locus_tag="IW671_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0898626.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IW671_001640"
     CDS             7468..8202
                     /locus_tag="IW671_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0898627.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IW671_001641"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IW671_001641"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0898628.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IW671_001642"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IW671_001642"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0898629.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IW671_001643"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IW671_001643"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0898630.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IW671_001644"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IW671_001644"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0898631.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IW671_001645"
     CDS             11787..13820
                     /locus_tag="IW671_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898632.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IW671_001646"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IW671_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IW671_001647"
     CDS             15353..16159
                     /locus_tag="IW671_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898633.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IW671_001648"
     CDS             16171..17391
                     /locus_tag="IW671_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898634.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IW671_001649"
     CDS             17381..18967
                     /locus_tag="IW671_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0898635.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IW671_001650"
     CDS             19006..21144
                     /locus_tag="IW671_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0898636.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="IW671_001651"
     CDS             complement(21512..22495)
                     /locus_tag="IW671_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0898637.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4712 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DACEKQ010000050        21502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEKQ010000050 DACEKQ010000000
VERSION     DACEKQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.053"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..400
                     /locus_tag="IW671_001652"
     CDS             2..400
                     /locus_tag="IW671_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0898638.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IW671_001653"
     CDS             384..1079
                     /locus_tag="IW671_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0898639.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="IW671_001654"
     CDS             complement(1663..2550)
                     /locus_tag="IW671_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0898640.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="IW671_001655"
     CDS             2735..3574
                     /locus_tag="IW671_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0898641.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="IW671_001656"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="IW671_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0898642.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="IW671_001657"
     CDS             5042..5398
                     /locus_tag="IW671_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898643.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="IW671_001658"
                     /pseudo
     CDS             5401..6558
                     /locus_tag="IW671_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6584..7024)
                     /locus_tag="IW671_001659"
     CDS             complement(6584..7024)
                     /locus_tag="IW671_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0898644.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="IW671_001660"
     CDS             7184..7690
                     /locus_tag="IW671_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0898645.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="IW671_001661"
     CDS             7858..9771
                     /locus_tag="IW671_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0898646.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="IW671_001662"
     CDS             9776..10777
                     /locus_tag="IW671_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0898647.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIEKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="IW671_001663"
     CDS             complement(10979..11185)
                     /locus_tag="IW671_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0898648.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="IW671_001664"
     CDS             complement(11831..12679)
                     /locus_tag="IW671_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0898649.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12843..13745
                     /gene="gnd"
                     /locus_tag="IW671_001665"
     CDS             12843..13745
                     /gene="gnd"
                     /locus_tag="IW671_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0898650.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="IW671_001666"
     CDS             13747..14748
                     /locus_tag="IW671_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0898651.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="IW671_001667"
     CDS             14745..15029
                     /locus_tag="IW671_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0898652.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="IW671_001668"
     CDS             15062..15859
                     /locus_tag="IW671_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0898653.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="IW671_001669"
     CDS             15873..16706
                     /locus_tag="IW671_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0898654.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="IW671_001670"
     CDS             16724..17581
                     /locus_tag="IW671_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0898655.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="IW671_001671"
     CDS             17603..18112
                     /locus_tag="IW671_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0898656.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="IW671_001672"
     CDS             18149..18550
                     /locus_tag="IW671_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898657.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="IW671_001673"
     CDS             18662..19015
                     /locus_tag="IW671_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898658.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="IW671_001674"
     CDS             19049..19492
                     /locus_tag="IW671_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0898659.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="IW671_001675"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="IW671_001675"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0898660.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="IW671_001676"
     CDS             20587..21300
                     /locus_tag="IW671_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0898661.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7398 a         3302 c         4490 g         6312 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttta aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata gaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc ca
//
LOCUS       DACEKQ010000051        20831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEKQ010000051 DACEKQ010000000
VERSION     DACEKQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.054"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(193..2190)
                     /gene="tkt"
                     /locus_tag="IW671_001677"
     CDS             complement(193..2190)
                     /gene="tkt"
                     /locus_tag="IW671_001677"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0898662.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2292..2543)
                     /locus_tag="IW671_001678"
     CDS             complement(2292..2543)
                     /locus_tag="IW671_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0898663.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2690..3313
                     /gene="lexA"
                     /locus_tag="IW671_001679"
     CDS             2690..3313
                     /gene="lexA"
                     /locus_tag="IW671_001679"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0898664.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3493..3711
                     /locus_tag="IW671_001680"
     CDS             3493..3711
                     /locus_tag="IW671_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898665.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4000..4512)
                     /locus_tag="IW671_001681"
     CDS             complement(4000..4512)
                     /locus_tag="IW671_001681"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0898666.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4526..6823)
                     /gene="recJ"
                     /locus_tag="IW671_001682"
     CDS             complement(4526..6823)
                     /gene="recJ"
                     /locus_tag="IW671_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0898667.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6965..7399)
                     /locus_tag="IW671_001683"
     CDS             complement(6965..7399)
                     /locus_tag="IW671_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0898668.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7409..8197)
                     /locus_tag="IW671_001684"
     CDS             complement(7409..8197)
                     /locus_tag="IW671_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0898669.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8194..9135)
                     /locus_tag="IW671_001685"
     CDS             complement(8194..9135)
                     /locus_tag="IW671_001685"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0898670.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9312..9899)
                     /locus_tag="IW671_001686"
     CDS             complement(9312..9899)
                     /locus_tag="IW671_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0898671.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10120..11433)
                     /gene="obgE"
                     /locus_tag="IW671_001687"
     CDS             complement(10120..11433)
                     /gene="obgE"
                     /locus_tag="IW671_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0898672.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11721..12731
                     /gene="gap"
                     /locus_tag="IW671_001688"
     CDS             11721..12731
                     /gene="gap"
                     /locus_tag="IW671_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0898673.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12816..13751)
                     /locus_tag="IW671_001689"
     CDS             complement(12816..13751)
                     /locus_tag="IW671_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0898674.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13937..14896
                     /locus_tag="IW671_001690"
     CDS             13937..14896
                     /locus_tag="IW671_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0898675.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14982..16076)
                     /locus_tag="IW671_001691"
     CDS             complement(14982..16076)
                     /locus_tag="IW671_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0898676.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16265..17026
                     /locus_tag="IW671_001692"
     CDS             16265..17026
                     /locus_tag="IW671_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0898677.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17141..17581)
                     /gene="fabZ"
                     /locus_tag="IW671_001693"
     CDS             complement(17141..17581)
                     /gene="fabZ"
                     /locus_tag="IW671_001693"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0898678.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17801..18556
                     /gene="fabI"
                     /locus_tag="IW671_001694"
     CDS             17801..18556
                     /gene="fabI"
                     /locus_tag="IW671_001694"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0898679.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18666..19679)
                     /locus_tag="IW671_001695"
     CDS             complement(18666..19679)
                     /locus_tag="IW671_001695"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0898680.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19844..20764
                     /locus_tag="IW671_001696"
     CDS             19844..20764
                     /locus_tag="IW671_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0898681.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6010 a         4472 c         3688 g         6661 t
ORIGIN      
        1 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
       61 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      121 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      181 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      241 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      301 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      361 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      421 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      481 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      541 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      601 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      661 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      721 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      781 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      841 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
      901 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
      961 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1021 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1081 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1141 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1201 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1261 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1321 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1381 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1441 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1501 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1561 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1621 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1681 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1741 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1801 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1861 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     1921 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     1981 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2041 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2101 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2161 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2221 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2281 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2341 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2401 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2461 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2521 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2581 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2641 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2701 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2761 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2821 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     2881 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     2941 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3001 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3061 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3121 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3181 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3241 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3301 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3361 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3421 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3481 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3541 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3601 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3661 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3721 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3781 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3841 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     3901 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     3961 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4021 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4081 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4141 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4201 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4261 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4321 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4381 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4441 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4501 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4561 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4621 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4681 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4741 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4801 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4861 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     4921 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     4981 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5041 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5101 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5161 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5221 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5281 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5341 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5401 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5461 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5521 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5581 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5641 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5701 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5761 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5821 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     5881 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     5941 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6001 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6061 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6121 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6181 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6241 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6301 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6361 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6421 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6481 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6541 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6601 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6661 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6721 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6781 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6841 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     6901 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     6961 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7021 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7081 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7141 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7201 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7261 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7321 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7381 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7441 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7501 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7561 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7621 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7681 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7741 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7801 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7861 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     7921 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     7981 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8041 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8101 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8161 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8221 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8281 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8341 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8401 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8461 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8521 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8581 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8641 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8701 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8761 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8821 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     8881 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     8941 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9001 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9061 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9121 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9181 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9241 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9301 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9361 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9421 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9481 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9541 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9601 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9661 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9721 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9781 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9841 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
     9901 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
     9961 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10021 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10081 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10141 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10201 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10261 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10321 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10381 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10441 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10501 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10561 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10621 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10681 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10741 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10801 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10861 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    10921 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    10981 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11041 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11101 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11161 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11221 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11281 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11341 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11401 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11461 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11521 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11581 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11641 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11701 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11761 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11821 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    11881 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    11941 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12001 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12061 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12121 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12181 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12241 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12301 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12361 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12421 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12481 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12541 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12601 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12661 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12721 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12781 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12841 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    12901 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    12961 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13021 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13081 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13141 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13201 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13261 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13321 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13381 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13441 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13501 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13561 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13621 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13681 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13741 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13801 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13861 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    13921 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    13981 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14041 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14101 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14161 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14221 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14281 aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg gccatcagga
    14341 aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata attatcctga
    14401 tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc atcataaaag
    14461 tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta aacccaacgg
    14521 ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct tccctaaaaa
    14581 cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga aagccattag
    14641 taaccaacat gatattgata tatattttgc ggaccctgga acaccttctc aacgcccatt
    14701 aaacgagaat tctaacggga ttctgcgtcg taatggactg ccgaaatcaa tggattttag
    14761 aaaagtgaat cagacattta tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa
    14821 atcattgaat tacagaacac caatcgaaat atttttgagc tatgtacaag aagcatttta
    14881 ttctagctta atttgacaaa tcataaccta taaaaaaaca aaataaaaaa gcagaaataa
    14941 ctggctattt ctagcggatt atttctgcaa taactaatta tctatttttt attttcaatt
    15001 aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga ataattcgtc atcggagatt
    15061 cttcctacaa cattgacacg tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct
    15121 cctttgtatt gaccgtaccc ttcattgtca atcagtacat ccccatgatg catatctcgc
    15181 gtattatgag gcgggaaatc tttatctttg tagtatacac gagtcatcgt tgaacgaagg
    15241 atatattctg agcgatcccc tcgataacta tgaaggttat caaacaagca gattcgttcg
    15301 ttttctgtaa taccttcttc cgtatctact ttcaatgttg gataatccgc gttaaatgct
    15361 tctgccattt ctttcaattc ttcttcactt gcgtacgcat ttccaataga aatatcatca
    15421 atcaatcctg taaggattaa atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct
    15481 tccagcgtgc acaagccatc ttgcgtaggc caagggccaa actcagcaga ttgcgaattg
    15541 acgaatgcca tcgtattcaa attatatttt ctgaattttt ctgaacaaaa aacaaagtgt
    15601 tcgtatccta aaccagagta tcgatgagga taaaaattat gagaacctaa taaattatcc
    15661 gtgtttggcg aataactcat gatgttgtca acgtaattcg tgcccgaact catattgatc
    15721 tcgattttga taccaaaagg attgcgcgtc atttttgctt cttctgctcc tgtgaatccg
    15781 atatctaagc ggaccccgta ggcacccatc ttatggaaaa aagataagtc atcgtaagaa
    15841 atctccaact gttcaaataa cgctggattg atatcaacca ttacttccat gcctaggcta
    15901 ttcgcgtaat caactacttc tttaaattct gaaagaactt tttctttatc atcattgatc
    15961 tgcagcaaac tggtaaaaac ccgtttatac ccatacttat gagctaaatc caagtacgct
    16021 ttgtcctttt caaatgttga ccgttctgga taaatcgaga ttcctaattt tcccatatta
    16081 ccatttcctc ctgcatcttt tcgtttttgt atccacttac attatagcaa acacaaagta
    16141 aatggtctac accttttctt tttcttttaa aataaatgat aaattccaat aatttaagta
    16201 ggtattttcc ctctattttg ttatcattaa agataacaaa atagtaaatt ggaaaggaac
    16261 agttatgaag aaaaataaag ttattttaag tattagttta gcagcagcat tgctgtttgc
    16321 tgctggttgt tctgcaaaca acacgacttc ttctagttcg tctacagaaa gttcaaaaag
    16381 tacatctact gcggaaagca cgactgccag tagcgtcgat ttgaacgcat tggaattacc
    16441 acaattgacg aaagatgtcg ccgaaaatga agatctggtc gaaatggtga cgaataaagg
    16501 cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac
    16561 acatgcaaaa gacggttact acaacggact gactttccat cgcgtcatca aagatttcat
    16621 gatccaaggt ggcgatccta aaggcgatgg cactggaggg gaaagcattt ggggagaagg
    16681 atttgaaaca gagatcaaca accaccttta taatataaga ggtgcgttat ctatggctcg
    16741 ctcacaagat cctaacagta acggcagcca gttctttatc gttcaaaata cagatgatat
    16801 gcatgatggt cttttaaaag acgactatcc tcaagcaatc atcgacgctt ataaaaacgg
    16861 cggttatcct tcactagatg gccattatac tgtcttcggc caagtgattg aaggaatgga
    16921 tgtcgtggat gctattgcaa atatcgaaac agatagttca gacaaaccaa aagaagaagt
    16981 aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg
    17041 aatcctgcat agatgacaac gtatgctagg attcaggcgt cttcttttta cataaaaagc
    17101 aagtaagctt ccttttgttt tgctatatca ggttttatag tcaagcattt tggttgacga
    17161 tgaacgtgaa ctcgcactca cagacttttt tatcatctac atatgctgca gataacgctg
    17221 taccgactga tcccttcatt ttggtgattt caaaatgaca tttcagcaca tctcctggaa
    17281 caactttttg acggaatttg gccttattga tcccaccgat ataagctctt tttcctagaa
    17341 actcttctga ttttaggata agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg
    17401 cacccggcat cactggattg tcaggaaagt gaccttggaa aaaatcttca ttgattgtta
    17461 cattttttgt cgcaacgatc ttttttcctg gttctaaagt atctacataa tcaatataac
    17521 aaataggata acgattagga atcaattcca ttacttcagt agcagataat acgtaagaca
    17581 taattaaact ccttctttca ggcaattgcc attttcatta ctttgactat caaagtatca
    17641 tattcctaaa tatttagcaa tcttttttta atttaagaaa aaatagtttg acaatcaaaa
    17701 cattttgact aataattaag aaatcaagaa gttattttag ctattattct ctattttttt
    17761 acgactatca aatttttatc agaaacaaag gagagtcatt atgtcatttc tagaaggaaa
    17821 aaaaatcgtc attatgggcg tcgctaacaa aaaaagcatt gcttggggtt gtgctgaagc
    17881 aatgttgaaa caaggggcag aaattatcta tacttaccaa aatgaacgaa tgaaaaaatc
    17941 attagtcaaa ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt ctagcgacga
    18001 aagcatcgaa aaagcaatga atgccatcgg tgaatatgct ggggagatcc acggccttgt
    18061 ccatgcagtt gcttacgcca acaaagaaga attgtccgga aatgtgtctg atatttccag
    18121 agacggctat caattagctc aagatatcag cagctactct ttaattgctg tatcaaaata
    18181 cgcacaaccg tttcttgcac caaacagcgg gatcgttagc atgacttatc taggttcaga
    18241 acgcgcaatc ccgaattata acatgatggg tattgcgaaa gcttctcttg aagctgctat
    18301 ccgttatttg gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa tctcagctgg
    18361 ggcaatcaaa acattagctg tcacaggtgt taaagactat caaaaactga tccagttatc
    18421 tgaatctcgt acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta atgcttgtgc
    18481 tttcttaatc agcccacttt ccagcggaat catcggtgat attatctacg ttgacaaagg
    18541 tgtccatctt tcataaactt ttgttgcatt ctttaaagta aaacatataa aaatcaggaa
    18601 aaaacttttt aaagtctctt cctgattttt tctttttacg tttttttcaa ctcattactt
    18661 tgaaattatt ccacgacttc aatccagcct tctggtgctt ctatgtctcc aaattgaata
    18721 cctgtcaatt catcatacaa ccgttttgtt actggtccta cttccgtttc actataaaat
    18781 acatgaagat tgttattgtg ataaatccca ccgattggag aaataacagc cgctgtacca
    18841 catgcaccag cttctgcaaa ttgatctaga tcatcaatga aaatatctcc ttcaattgct
    18901 tgcagtccta gtcgttcttc agctaactgc aacaaggaat atttcgtgat acttggtaag
    18961 atagagggcg attgcggagt gatgaatttt ccgtcttttg taattccaaa gaaatttgct
    19021 gcacctactt cttcgatttt cgtatgtgtg gctggatcaa gataaacaca atctgagtaa
    19081 tccagttctt ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc gccgactttt
    19141 gctgcaccag tccccttagg agcagcacga tcgtaatcag atacaataaa attcgttggt
    19201 tttagtccac ctttgaaata cgctcctact ggcgtacaga agacgacaaa taaatattct
    19261 ggagctggat ggacaccaat gttttcaccg accccaatca gtaaagggcg caagtataat
    19321 gtagcgcctg ttccataggg cggcacaaaa tcttcatttg cttttaccac ttgcttgaca
    19381 gcatcaacga acatcttttc agggaccatt ggcatcagca atcgtctagc actttgattc
    19441 atccgtcgac tattttgttc aggtcgaaag agattgacct tgccgtcttt tcgacgataa
    19501 gcttttagac cttcaaaaca ttgctgacca taatgaagcg ctggagagcc ttcatgaatc
    19561 gtcaggtaat tatcttctgt caattctcct tcttcccatt gtccgtcttt ccatttggca
    19621 ataaaacgcc aaggagtttt gatataagaa aaacctagat tgttccaatc gatttgcata
    19681 tacttaccac cttcgttatt ttctccacat tttatcatta tccatattat ttgtcatttt
    19741 attttcagct tttttccttc attatagtta tttttgctat ttcctttttt gaaaacttct
    19801 tggtacaata cagaaagaat caaattttag aaaagaggat tttatgtttt cattattagc
    19861 aactgccggt acgacagact cttctggcga gacattgaca gatcaagctg tcagcaaagt
    19921 aaatgcgttt cagcggtttt gggacgggat tgattgggac gccattttgg ctgcctttat
    19981 ccaaaaaagt ttatatctga tttttttgat cgtccttttt tggattcttc accgtgtggg
    20041 aaaatatctg attgataagt cttttaatca gtattctaaa aaagccattc taaacgagag
    20101 ccgcttaaag actttacata gtcttttaaa tactattttt cattataccc ttggtttctt
    20161 ttttatctat gcaattttat ctgctatcgg tgtgccgatc ggatcacttt tggctggtgc
    20221 cggaattgca ggtgtggcaa tcggtcttgg tgcccaaggg ttcatgagtg atgttattac
    20281 cggatttttt attatcatgg aacaacagat ggatgttggg gactacatca agctagcaaa
    20341 cttaacgatt gaaggaaccg tcgtatctgt tggtatccgt acccttcagc tgaaagcaac
    20401 ggatggtact gtccatttca tcccgaatcg aaacatcacc acgatcagca atctctctcg
    20461 tgcaaatatg caagtacttt tagatattcg gattgttcct gaagaaggat atgacaaaat
    20521 ctatgagatc attgatcgag taaatcgtac attagctgaa aaataccaag aagagctcca
    20581 aactgaacct tctatctttg gattagtgga tcttggaaac agcaattttg cgattcgtac
    20641 ggtctgctac gcattgaacg gaaaacaata tgccttaaaa gaagagttct tgagctccta
    20701 tgtcaaagaa ttgacagctt ctggtttcac gattccgaac agtccaattg ctgtcggaaa
    20761 ataatttgga caaacgatgc aacgaatctc actaaaaaga gcgtgggaca aaagtaaaga
    20821 acacttttgt c
//
LOCUS       DACEKQ010000052        20801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEKQ010000052 DACEKQ010000000
VERSION     DACEKQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.055"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW671_001697"
     CDS             <1..300
                     /locus_tag="IW671_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0898682.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IW671_001698"
     CDS             complement(724..894)
                     /locus_tag="IW671_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0898683.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IW671_001699"
     CDS             complement(1088..1267)
                     /locus_tag="IW671_001699"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898684.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1654..2766
                     /locus_tag="IW671_001700"
     CDS             1654..2766
                     /locus_tag="IW671_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0898685.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IW671_001701"
     CDS             complement(2844..3137)
                     /locus_tag="IW671_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0898686.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IW671_001702"
     CDS             3305..3619
                     /locus_tag="IW671_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0898687.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IW671_001703"
     CDS             3640..5619
                     /locus_tag="IW671_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0898688.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IW671_001704"
     CDS             5632..5958
                     /locus_tag="IW671_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0898689.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IW671_001705"
     CDS             5974..6492
                     /locus_tag="IW671_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898690.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IW671_001706"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IW671_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0898691.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IW671_001707"
     CDS             complement(7928..9433)
                     /locus_tag="IW671_001707"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0898692.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IW671_001708"
     CDS             complement(9525..10115)
                     /locus_tag="IW671_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0898693.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IW671_001709"
     CDS             10294..11940
                     /locus_tag="IW671_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0898694.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IW671_001710"
     CDS             11989..12708
                     /locus_tag="IW671_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0898695.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IW671_001711"
     CDS             13187..14134
                     /locus_tag="IW671_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0898696.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IW671_001712"
     CDS             14146..15066
                     /locus_tag="IW671_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0898697.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IW671_001713"
     CDS             15107..16564
                     /locus_tag="IW671_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898698.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IW671_001714"
     CDS             16909..17523
                     /locus_tag="IW671_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0898699.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IW671_001715"
     CDS             17516..19240
                     /locus_tag="IW671_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0898700.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IW671_001716"
     CDS             19237..20688
                     /locus_tag="IW671_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0898701.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACEKQ010000053        19996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEKQ010000053 DACEKQ010000000
VERSION     DACEKQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.056"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW671_001717"
     CDS             4..816
                     /locus_tag="IW671_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0898702.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW671_001718"
     CDS             813..1613
                     /locus_tag="IW671_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0898703.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW671_001719"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW671_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0898704.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW671_001720"
     CDS             3110..4819
                     /locus_tag="IW671_001720"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0898705.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW671_001721"
     CDS             5031..9383
                     /locus_tag="IW671_001721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0898706.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW671_001722"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW671_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0898707.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW671_001723"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW671_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0898708.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW671_001724"
     CDS             11221..11514
                     /locus_tag="IW671_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0898709.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW671_001725"
     CDS             11511..11822
                     /locus_tag="IW671_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0898710.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW671_001726"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW671_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0898711.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW671_001727"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW671_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0898712.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW671_001728"
     CDS             complement(14623..14844)
                     /locus_tag="IW671_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0898713.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IW671_001729"
     CDS             15292..17655
                     /locus_tag="IW671_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0898714.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IW671_001730"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IW671_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0898715.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IW671_001731"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IW671_001731"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0898716.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3513 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DACEKQ010000054        19809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEKQ010000054 DACEKQ010000000
VERSION     DACEKQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.057"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            190..1086
                     /locus_tag="IW671_001732"
     CDS             190..1086
                     /locus_tag="IW671_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0898717.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1236..2177)
                     /locus_tag="IW671_001733"
     CDS             complement(1236..2177)
                     /locus_tag="IW671_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0898718.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2303..3424
                     /locus_tag="IW671_001734"
     CDS             2303..3424
                     /locus_tag="IW671_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0898719.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3682..5184
                     /locus_tag="IW671_001735"
     CDS             3682..5184
                     /locus_tag="IW671_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0898720.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5251..5778
                     /locus_tag="IW671_001736"
     CDS             5251..5778
                     /locus_tag="IW671_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0898721.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5989..7050
                     /locus_tag="IW671_001737"
     CDS             5989..7050
                     /locus_tag="IW671_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0898722.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7053..7949
                     /gene="murQ"
                     /locus_tag="IW671_001738"
     CDS             7053..7949
                     /gene="murQ"
                     /locus_tag="IW671_001738"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0898723.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7963..9945
                     /locus_tag="IW671_001739"
     CDS             7963..9945
                     /locus_tag="IW671_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898724.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10010..10456)
                     /gene="nrdI"
                     /locus_tag="IW671_001740"
     CDS             complement(10010..10456)
                     /gene="nrdI"
                     /locus_tag="IW671_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0898725.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10667..12091
                     /locus_tag="IW671_001741"
     CDS             10667..12091
                     /locus_tag="IW671_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0898726.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12272..13045)
                     /locus_tag="IW671_001742"
     CDS             complement(12272..13045)
                     /locus_tag="IW671_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0898727.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13042..13947)
                     /locus_tag="IW671_001743"
     CDS             complement(13042..13947)
                     /locus_tag="IW671_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898728.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14101..14421)
                     /locus_tag="IW671_001744"
     CDS             complement(14101..14421)
                     /locus_tag="IW671_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0898729.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14895..15581
                     /locus_tag="IW671_001745"
     CDS             14895..15581
                     /locus_tag="IW671_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0898730.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15583..16527
                     /locus_tag="IW671_001746"
     CDS             15583..16527
                     /locus_tag="IW671_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0898731.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16726..17370
                     /gene="pcp"
                     /locus_tag="IW671_001747"
     CDS             16726..17370
                     /gene="pcp"
                     /locus_tag="IW671_001747"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0898732.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17449..18726
                     /locus_tag="IW671_001748"
     CDS             17449..18726
                     /locus_tag="IW671_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898733.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(18910..19659)
                     /locus_tag="IW671_001749"
     CDS             complement(18910..19659)
                     /locus_tag="IW671_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0898734.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         6366 a         3500 c         4180 g         5763 t
ORIGIN      
        1 acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa
       61 tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt
      121 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
      181 gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt
      241 ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
      301 attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
      361 tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt
      421 ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
      481 gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat
      541 aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta
      601 ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt
      661 ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct
      721 ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa
      781 ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa
      841 aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat
      901 atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt
      961 aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt
     1021 atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac
     1081 aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat
     1141 ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca
     1201 gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct
     1261 tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt
     1321 ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc
     1381 aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca
     1441 atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca
     1501 aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca
     1561 atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc
     1621 caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact
     1681 tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct
     1741 ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
     1801 ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca
     1861 tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata
     1921 tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg
     1981 atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca
     2041 gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg
     2101 acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca
     2161 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
     2221 gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt
     2281 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
     2341 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
     2401 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
     2461 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
     2521 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
     2581 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
     2641 tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
     2701 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
     2761 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
     2821 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
     2881 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
     2941 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
     3001 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
     3061 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
     3121 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
     3181 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
     3241 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
     3301 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
     3361 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
     3421 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
     3481 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
     3541 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
     3601 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     3661 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
     3721 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
     3781 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
     3841 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
     3901 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
     3961 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
     4021 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
     4081 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
     4141 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
     4201 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
     4261 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
     4321 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
     4381 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
     4441 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
     4501 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
     4561 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
     4621 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
     4681 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
     4741 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
     4801 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
     4861 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
     4921 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
     4981 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
     5041 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
     5101 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
     5161 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
     5221 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
     5281 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
     5341 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
     5401 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
     5461 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
     5521 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
     5581 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
     5641 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
     5701 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
     5761 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
     5821 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
     5881 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
     5941 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
     6001 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
     6061 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
     6121 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
     6181 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
     6241 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
     6301 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
     6361 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
     6421 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
     6481 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
     6541 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
     6601 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
     6661 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
     6721 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
     6781 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
     6841 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
     6901 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
     6961 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
     7021 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
     7081 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
     7141 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
     7201 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
     7261 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
     7321 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
     7381 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
     7441 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
     7501 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
     7561 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
     7621 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
     7681 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
     7741 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
     7801 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
     7861 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
     7921 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
     7981 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
     8041 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
     8101 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
     8161 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
     8221 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
     8281 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
     8341 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
     8401 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
     8461 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
     8521 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
     8581 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
     8641 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
     8701 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
     8761 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcgttgat tgccatcggt
     8821 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
     8881 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
     8941 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
     9001 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
     9061 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
     9121 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
     9181 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
     9241 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
     9301 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
     9361 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
     9421 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
     9481 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
     9541 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
     9601 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
     9661 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
     9721 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
     9781 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
     9841 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
     9901 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
     9961 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
    10021 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
    10081 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
    10141 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
    10201 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
    10261 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
    10321 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
    10381 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
    10441 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
    10501 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
    10561 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
    10621 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
    10681 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
    10741 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
    10801 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
    10861 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
    10921 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
    10981 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
    11041 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
    11101 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
    11161 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
    11221 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
    11281 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
    11341 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
    11401 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
    11461 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
    11521 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
    11581 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
    11641 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
    11701 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
    11761 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
    11821 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
    11881 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
    11941 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
    12001 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
    12061 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
    12121 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
    12181 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
    12241 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
    12301 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
    12361 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
    12421 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
    12481 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat
    12541 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
    12601 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
    12661 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
    12721 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
    12781 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
    12841 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
    12901 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
    12961 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
    13021 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
    13081 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
    13141 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
    13201 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
    13261 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
    13321 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
    13381 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
    13441 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
    13501 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
    13561 gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc
    13621 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
    13681 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
    13741 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
    13801 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
    13861 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
    13921 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
    13981 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
    14041 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
    14101 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
    14161 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
    14221 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
    14281 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
    14341 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
    14401 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
    14461 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
    14521 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
    14581 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
    14641 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
    14701 agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag
    14761 taagataatt tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt
    14821 gagaaaacaa ctatattttt tatgtgtttt gctttataat taaaaaaata ttgtcatttt
    14881 atggaggaaa atatatggaa aattatctta gccttttagg cgtcttgatt gttattctcg
    14941 ggtttgcatt taaattagac tcgatattga tcgtaatggt tgctttagtt gttacagctt
    15001 tgactggcgg tcttggcatt caaggaatgt tagaactttt aggtacaagt tttgtaaaca
    15061 atcgcgggat ggcgattttt atcatcatta tgttggcaac aggaacgctt gaaagaaatg
    15121 gtctgaaaga atcagcggct tccttgatca agcgattcaa gaaagtatct gctggtatga
    15181 ttgtagatat ttatgggatc tttcgaatga tctttgcggc ttttaacgtg agttttggtg
    15241 gagttgctgg atttgttcgt ccaatcattt taccgatggc attggggacc atcgaatcaa
    15301 aagggttaga agtccatcct aagcatgaag aagaactaaa aggtatggct tcagctatgg
    15361 aaaatgtctg ctggttcttt ggccaagtac tattcatcgg aggagcaggg gcgttgctag
    15421 tccaatcaac actgaaagat ctaggatatg acgtaacatt gggacagctt gctttagtgg
    15481 aagttccagt ggctatcacg gctttagttg tggcaagtgt ctacttctat cttaaagaaa
    15541 agaaattagc gaagaaatat tatccaggaa aggggcaata agatggactt ttttacaaat
    15601 cctgatattt tattaggcga taaattactg gagatccttt atatcgtgat gggactcgta
    15661 ttgatttaca ctggagtaag aaatttaaca gataaaacca atcctcatcg ttatgggtca
    15721 gcctttttct ggacggtctt aggggtagtt attgcaggag gaagatggct tccggcaatc
    15781 gtgaacggtg gcttgatctt tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa
    15841 ggagaaagcc ggcttccatc aaaagaatat atggaaaaaa tgtcagacaa actaggaatg
    15901 aaaattttta ttccagcttt gagtatcggt gtctttgcga tcttgtttgc tctatttacc
    15961 aatttaggtg ctttagtcgg cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg
    16021 ttcttttctc gagataatcg accaagtaca tttttagatg atgcagcaga tatgctgggc
    16081 acagtagggc cgttaagcat gttgccaatg cttttagctt ctctaggtgc ggtatttaca
    16141 agcgctggag ttgggacagt gatttccaat gcagtaggta cgatcgttcc agaaggaaat
    16201 gtgaacatcg gcatcatcat ttacgctctt ggcatggtga tatttacggt aatcatgggg
    16261 aatgcatttg cggccatcac cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt
    16321 catggagcga atccagcgtt aataggaatg gttgcattga cttgcggttt ttgtgggacc
    16381 ctgctgacac cgatggctgc taacttcaat atcgtgcctg tagcgatgct ggagatgaaa
    16441 gacaaatacg gagtgatcaa aaaccagttg tttattgcgt tatttatgct agttttccaa
    16501 attgcttata tgattctatt taaataatag aagagagggg ttgtgacaga ataattgtcg
    16561 caacctcttc taacattgta gtggaacact atttttttat aagaatatat taagtttgat
    16621 ctgttatgtt atttaaacta ttgtttcctt tatttacgct gttcaaggaa aatggtatga
    16681 taaaagataa ccatatgtta aatcaatcgg acggaggaac aacagatgaa agtactagta
    16741 acaggttttg atccatttgg tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa
    16801 atgccagatg aaatcagcgg tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa
    16861 aaaagcagtc aagtagtcaa agaagccatc caacagcacc aaccagatgt cgtgatctgt
    16921 gtcggacaag caggtgggcg gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca
    16981 gaagcaagaa tccctgataa tgaaggaaat cagccattcg atacggcttt agaagaaaac
    17041 ggtccagccg cttactttac ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat
    17101 catggtctac cagcgtacat ctcttataca gcaggaacat ttgtctgcaa tgatatcatg
    17161 tatcgtttgc ttcatttgat cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac
    17221 gtgccattct cacccgatca agtgatcgat cgtccagtgg gtacgccttc tatgtcttta
    17281 gaagacattg cttcttcttt gacttatgca gttgaagcag cagtagaaaa taaagaagat
    17341 atttccggaa atgccggaac aactcactaa tttagcaaga aacactggct ggttgctaac
    17401 gttatgttag tagccagctt tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg
    17461 caatccgtct ttgaagaatg gtttccaaaa gaaatcagca aaagctatcc catcaaaatt
    17521 tctaaacaat acacaagcag tcaacgttgg gagatctatg caaaattaac gaaaaaacag
    17581 cgggaactag tcgataagca ccgccgatat ctaatcagct ctcgttttat ggaagaacat
    17641 tatttagctg caacagattg ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca
    17701 aaacgaagcc agcaaaaatt gtattgtgag tgtggtagag aattaaaagt gcaatacatc
    17761 gttaaatcgc caaaaaccgg aaagatccta aaactaggca tcaaccactt tgccgatcac
    17821 ctgcatgtct ctccgacagt ggcagcttcg atccatcagg gaatgacaaa agttgatctg
    17881 gccttagacg aattattatg gcttaagcag aagaatatcg attttcctga aggactatgg
    17941 caaaaatact gttttgtctt gtaccaaaat cgtcggatga aacagccata tttaccggat
    18001 ataaaattag ctcagcggct cgcagaattt cgccaagtag aaatgccgat ttacatagcg
    18061 gattatcaag cattggaaaa tgaaatcaag aaaataagtg aacacatcaa tgggcagtca
    18121 aaaaagcgac agataaaaaa agaactattt gacgactttg cagaagaatt agtaaaggat
    18181 gtagaagaat tcttgatcaa ctatcgcgca tttttacgta aagattggca atccatcgta
    18241 tatgaagaag tgccagtaca tcctaatgct tattttgaaa catttatttc cgtgttgaga
    18301 aagacaaaac gtcaacgaac accagaagtc actgctcaga tggaatattt tgcgaaaaac
    18361 caacgattca tccaaccaaa aatctatcta tttatttgga agcaatattg tcactatggt
    18421 tttacagaag ggttcttcga cagcattcct cggatagtca gaaatggttt tttaaaagta
    18481 ttgcgtaaag aacgagaagc tatacaatct gctgataaga aagaccgtac agtctcaaaa
    18541 gaaaaatggc aactagtagt gaaagatatc caatctggaa atgtgcaaga gacaatcgac
    18601 aaatggaaag gaaaacatta tcgttttact gaagctcaga aacaagcgtt ggaatattat
    18661 cagaagttag aagaatcact gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta
    18721 ctataaaaaa tagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac
    18781 gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgat
    18841 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
    18901 tcagtttttt tatgcgtgtt gttgtgcgtt gtagtattct tcttccattt tttgcatacg
    18961 ttcacgttct ttttggttat accaaggtga aacggtaaat gcaaggacat cattcagcaa
    19021 gtaaacagag ctgttcacga acatcgccaa acttgcagat ccatgcgtaa atgaaatata
    19081 ccaaaggacc atttgagcaa gccctgaagc taaccaccag taatattggt tgttgtaacg
    19141 caagaaactg atgataccag cagtcaagct aatagaaaaa gcaatggcat ctacccacgg
    19201 ccgaggatca tctgtataac gaccaatcag gaatcctgaa acaagataga caaggaaagt
    19261 actgatgatt gcgataaacc agctttttcc agtgaatttt cggatttttg aagccatatt
    19321 atgattccat tctttactta acaatacggg gatatccaat gtgatcatat aagcaatctg
    19381 ttcaccgata cttaaatagt tctttgctga aaacccaacg ataataaaac aaatcgcaga
    19441 caaaatacct aaaataccgt tgactgattt tgccgcattg atggataaaa tacacaatac
    19501 accaagcgtg gtaccgataa atgtaataac ggacagccaa gtgatgggac tattgacaag
    19561 agtcatgacc tggcagccaa gactgaaaaa gaaaagataa taattttgtt gcggccagcc
    19621 ttttagctga tgagctaaaa actgaaaata tgacttcatg tatgaggact ccttaagaca
    19681 ctaaatgtag tgtgttctgt atttactaac acaatctatt gtatgctttt cttgaaaaaa
    19741 aactagtctt tcaattataa ataatttgtg ttatgataat tttctgtttg ttttcttgaa
    19801 aatataatt
//
LOCUS       DACEKQ010000055        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEKQ010000055 DACEKQ010000000
VERSION     DACEKQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.058"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..3655
                     /locus_tag="IW671_001750"
     CDS             128..3655
                     /locus_tag="IW671_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0898735.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3652..7374
                     /gene="addA"
                     /locus_tag="IW671_001751"
     CDS             3652..7374
                     /gene="addA"
                     /locus_tag="IW671_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0898736.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7443..7784
                     /locus_tag="IW671_001752"
     CDS             7443..7784
                     /locus_tag="IW671_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898737.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8093..9136
                     /gene="pheS"
                     /locus_tag="IW671_001753"
     CDS             8093..9136
                     /gene="pheS"
                     /locus_tag="IW671_001753"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0898738.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9141..11561
                     /locus_tag="IW671_001754"
     CDS             9141..11561
                     /locus_tag="IW671_001754"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0898739.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="IW671_001755"
     CDS             complement(11731..12741)
                     /gene="trpS"
                     /locus_tag="IW671_001755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0898740.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12995..13732
                     /locus_tag="IW671_001756"
     CDS             12995..13732
                     /locus_tag="IW671_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898741.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13746..14561
                     /locus_tag="IW671_001757"
     CDS             13746..14561
                     /locus_tag="IW671_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0898742.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14574..15221
                     /locus_tag="IW671_001758"
     CDS             14574..15221
                     /locus_tag="IW671_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0898743.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15237..15893
                     /locus_tag="IW671_001759"
     CDS             15237..15893
                     /locus_tag="IW671_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0898744.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16055..16876
                     /gene="racE"
                     /locus_tag="IW671_001760"
     CDS             16055..16876
                     /gene="racE"
                     /locus_tag="IW671_001760"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0898745.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16879..18234
                     /gene="rph"
                     /locus_tag="IW671_001761"
     CDS             16879..18234
                     /gene="rph"
                     /locus_tag="IW671_001761"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0898746.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18235..18753
                     /locus_tag="IW671_001762"
     CDS             18235..18753
                     /locus_tag="IW671_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0898747.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18860..19360
                     /locus_tag="IW671_001763"
     CDS             18860..19360
                     /locus_tag="IW671_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0898748.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5392 t
ORIGIN      
        1 gcgtatactt aaaaaagtag aaatatcaga accttaaaag gatataattt gaagaatata
       61 gaacaaatgt tcagtatttt ttgatataat aaaggagcac cgtatcatgt gttgaaggag
      121 gaaaagcatg tctttacaat ttattcttgg aaacgggaaa aaagatcacc gtcaagtctt
      181 gatcgacgaa gcatccgctt ggctcgacca ggatgcaagt aaccaagtat ttttccttgt
      241 tcccaactac agcaaattcg aacaggaaca agaaatttta gcggagatgc gaagaagaag
      301 cggaaaaaaa actttcagca caacaaacgt tcaagtattt agtttttatc gtctggcttg
      361 gtatttgctg caacagacaa ctcttcttac aggaaatgaa ttatcagaat caggatctgc
      421 aatgatcttg cgacagattt tagaaaaaaa tgaggaagaa ttaacaatct ttcgcggaga
      481 aatcaataaa agagggttta ttcgtcaatt acaagaactc tacagtgaat tgcgtacagg
      541 aaatattcaa ccagaagacc tgatgctttt atttggtgat tcacctaatg cagaagacca
      601 gcaacttaaa atgaaagact tgaagctgat tttttcagct tatgacagcg aattgactca
      661 acgtgcacta acaaatgaag atgcactatc tatactagca aattatatgc agacacaaga
      721 tttcaacaac gtcaaattta ttgtcagtgg tttttctcga atcaatggtc aagagtatca
      781 attactgcag gcaatgatgg aaaaggggca attggttgtc gatttattgc tggatcgtcc
      841 ttatacagcg gatcttccag aagtacttac gctctttcat gagactggaa agctttattt
      901 ccgtcttttt caaagtgcaa gagaacggaa agtccctata ttagtggacc gatttgcacc
      961 agagcagact cgtccgacag agcttcaact gctgcaaaca atgtgggaag agacatctag
     1021 ccaaaagaaa gcggcagtac acaaaatagg gggacaagag catctccatt tgtgggaagc
     1081 tgaaaatcca actgaagaag tccgacaatt agctgttgaa atcagacgac tagtcagtga
     1141 agaaaactac cgttatcgtg atatccaagt gctgacaaaa aatatgtctt tatatggaaa
     1201 tatccttcgg ccgatctttc aagaaatggc aattcctttt tatatcgatg aagaacaaat
     1261 gatggaaaat catccgctga tcgaatggat caacagtctg ttcgctcttg accgctattc
     1321 ttatcggcta agtgatatta tgcgtttttt caaaactgaa ttatttctgt cgacggaagc
     1381 agaaaccgaa cttgatgaat ggaaacaaga gcgagatgaa ttcaggaaaa aactggacat
     1441 cactgaaaat gtagcacttg cttacaatta tcaaggaact tattggactc gtgaaaaaga
     1501 ctggcagttt gtggcgtatg attttgaagc agaacagatt gaagacgtac agtatttgga
     1561 agaacagtca aatacgatta gacgattatt tcagaaagct gtaccagtat ttttgaaaaa
     1621 aataaagaaa agcaaaacag gacaagaggc cgcaaatgtt ttttatcaat ttttgatcca
     1681 aagtggcgta cagcaacaat tattgttttg gcgtaatcag gaagtagaca gaggaaactt
     1741 agaagcggca agaaaccatg agcagacatg gggagcgctg attgatctgt tggatgaata
     1801 tgtagtaatt tatggtgaat cggcttttga ctggtcactg ttccaagaaa tcattacaag
     1861 tggtctagaa aatctaagtt atggtaaaat ccctaccgca atcgaccaag tgcgaatcaa
     1921 tcgtttggaa ttagttcgac caaatcaagc tgccgtcact tttgcattag ggttgaatga
     1981 tcaagtgttt ccagaccgaa aagagacaaa aggattgttg tccagtgaag aacgagaagt
     2041 attaaatcag gccttgccag atggacagtt tttattcgac ccatcaaagg aaagtatctc
     2101 ttttgagcct ttccaagcct atcttgtttt tctttccgga acagatcgat tatatcttag
     2161 ttatgcacaa agctatgaca cagatggttc gctgaagatg tctccttatt tgcgtagaat
     2221 cacagaatat ttatctgttc ccgtcgagag aaaagagcat ttgacgatcg aaagcgaccc
     2281 taattgttat gtagggactt acagaagcgg aatcaataca gtcaatagga tttatcgatt
     2341 agctttagat gaaaaaaaac ctgtaccaaa gcactggcaa aagctcaaag aattagtact
     2401 gaattcgtca tggcgaaatt ttgctttaca gatttttgaa agtcaggaac atttaaatgt
     2461 tcctgtttcg atgccagaaa aaatggcgga agagctttat ggaaaagaga tttatacttc
     2521 aatttcacgt atggagaatt tttataattg tgaatacaaa tacttcatgc aatttggttt
     2581 gaaattgaaa gaacgaactg tttatggtct aactccagca gcaactggtg atttttatca
     2641 tgaatcattg gatcgtttct tcaagctact gttttcaaac caattatcct tagtgaatat
     2701 gtccgatgcg cagcgtagag actttactga aaaagtgctg caggaagtat tcggagaact
     2761 tcggtttgag atattggata gttctgcaag aatgaactat atccgctacc agttaggaca
     2821 gacgattcaa aaagtgacat gggctttgca aaaacagagc aaaaatacag gcatgcatcc
     2881 tcttcagaca gagattctat ttggtcagat tgctggagca aaaggtatca caggtctgga
     2941 attaccgttg agtaatggtg gaaaactcca tgttcgaggg aaaatcgatc gaatcgatgt
     3001 agcttctgaa caagaagata cttggctaag cgtcgttgat tacaaatcta gcggacgttc
     3061 attcgatgtc acagaagctt attatggcat ggctatgcaa ttattgacgt atctggacgt
     3121 agcattgatg gatgcggtac gtttgactgg aaaggcggct gttaaaccag caggatcata
     3181 ttatttacat gttcataatc ctgtattgac ggagacagat gatgtagaga agaatacatt
     3241 gaaaaaattt agttatgatg gcgtttttgt cgatgatccc gctcttttag aagtactaga
     3301 tcattcactg gaagcaaaag aacaatcact gatctatcca gtgaaaaaaa atgcgaaaga
     3361 tgaataccaa aaaggtagtt tcagtaaaga caaatttttc acggaagaag aactgcaact
     3421 attcatggcg cataatcggg aaaatatgcg gttagctgga gatgcgatta cttccggaga
     3481 aatcaaacta aatccagctt ataaagataa acagcggatt gcctgcgaat tttgtccatt
     3541 cagaagcatc tgtacgtttg atgtgatgct gaaagaaaat aattaccacc gtctggagaa
     3601 aatagataag gaagaagcac tgcgtagaat attgaaaagg agtgaagaag agtgaatcaa
     3661 attcctttaa aaccgcaaaa tgaacgattc acagatgatc agtggcaagc gatttttgat
     3721 caaggagata atctattagt ctctgcgtct gccggatcag gtaaaactac agtgcttgtt
     3781 cgaagagtga ttgaaaaact aaaaatgggt tttgatatcg atgaattatt gattgtgaca
     3841 tttactgaag ctgccgcaag agaaatgaaa gaaagaattc aagaggcatt acaagaatcc
     3901 gtcaatagcg aaagtgaccc tgtacgtaga cagcatttta ctaagcagtt agttttattg
     3961 cccacagcga atatctcaac cttacatgct ttttgcctga cagttattcg acgttattat
     4021 tacttaattg atattgatcc agtctttcgc atgttaacag atgaaacaga aacgatcttg
     4081 atgaaggagg atgtgtggga cgagctgaga gaagcacttt atgcagaaaa tgatgaacga
     4141 tttttccaat taacaatgaa tttttctaat gatcgttcag atgacggctt gacgaatctg
     4201 gtgttttctc tttatgagtt tgcccgtgcc aaccctgacc cccaaaaatg gctagaacag
     4261 ttaagcgata attatcgttt gcctgagggg ctagcgaaat ctcgattata tcaaaaacaa
     4321 atccgtcctt tagttttggc agatatatac caatgtgtac agttatatga acagatgact
     4381 cagttagcac aaggtgaagg attagaaaaa atgcatgagc aagtagctgg agaacaacag
     4441 cagatcaaga atatctatga agcgttctcg caagatcggt tagaagaagc ttatgctggt
     4501 ttggaacaac taactttttc aacttttaag agcagtcgga aagcagaatt gaaagaaatt
     4561 tccaatgaag tcaagggcat gagggataaa gcaaaaaaac tgattcagca gataagtaaa
     4621 agttattttc ctgtttcccc aagccaaatg gaagaattaa cggacaaagc actaccgtta
     4681 gtcgaagaaa tgacaaaagt cacgcaaagc ttcatggacg gtttcagtat gcgtaaacgg
     4741 gaaaaaggtg tgctggattt caatgatctg gaacatttag cgttgcagat tctcacagaa
     4801 aaaacgaaag atgtatggct gccaagtgaa gcttcaaaac attatcgaaa aaaattcaaa
     4861 gaagtaatgg tagatgaata tcaagacgtg aaccaattac aagaagccat tttatactgg
     4921 ctaagagaac ccgatgatac aaaaggaaat atgttcatgg ttggggatgt caaacaatcc
     4981 atctattctt tccgtttagc agatcctagt ttatttatcg ggaaatacga gaatttctca
     5041 aagaaagaag gcggacgtcg aatcgttttg gctgagaact tccgctctag aaaagaagtc
     5101 ctttctttta cgaatcttat ttttgaacaa ctaatggatc cagcagtggg tcaaatcaat
     5161 tatgacgaag cagccaaact gatccaagga ttttcagact ttccagagaa tgaacagttt
     5221 gagccggaaa tcatgatcta tgaaaaagaa caggaagaat cagagattga gattccgacg
     5281 gatgatatac tggaagacaa aacagaaggc gaacttttta tgacaggact aaaaattcgt
     5341 caattaattg attcctcctt tatgatttat gataaaaaat caaaaaaaag tcgtccgata
     5401 gagtacaaag acattgttct cttgacacct acaaagaaga acaacttaac gattttagag
     5461 attttcaaaa cattggatat tccattagag atgaatgatg ctcaaaacta ttttcaagcg
     5521 acagaaattc gcacgatgat ctcgctgcta caattaatcg ataatcccta tcaagatatt
     5581 ccgcttgcgg ctgtattgcg ctcaccgatc gtgggtctga ttgaaccaga attagccagc
     5641 attcgattag ctaaccgtgc acatacttac tatgatgctg tgcttgctta tcaagcatca
     5701 aacgaagatg aattagcagc taagctggaa cattttggca agcaactaga gcattggcga
     5761 gaacttgctc gccgttcttc gatcactgac ttgttatggg atatctatta tgagacggga
     5821 tatctagagt atgtggtcgg tctgccagca ggggcacaga gacaagccaa tctttatgca
     5881 ttggttgatc gtgccaaagc ttacgagcaa agttctttca gaggattata ccagttcgtg
     5941 cgtttcattg aaaaaatgca agaaaaagat aaagacttag cagaaccagt catttcaata
     6001 gaagataatg ctgttcgcgt tatgacgatc catgcaagta aaggcttgga attcccagtt
     6061 gtatttttgt tagatatgac aaaggagttc aacttacaag acttgcgcaa tcggtatgct
     6121 tttgaagaaa aacttggagc aggaattcgc tatatggatc ctgaaacacg agtgttatat
     6181 gatacgttac cgtttcaagc catcaaattg gcaaagcaaa acaaattgtt gtcggaagaa
     6241 atgcgtaaat tgtatgtagg attgactcgg gcagaacaaa agcttttcat tgtcggttcc
     6301 tataaaaata aagagcaaat gatccaaacg tggtctgaag cagctgatca tgaggagtta
     6361 gtttttgatc ctgcgctacg tttgaagggc agaagcagct tgatgaattg gattggttac
     6421 ggattgatcc gtcatcctga aatgcagaaa tacttagagg aagaaatttc gacttctctt
     6481 cttcaacatt caaatgcaca gttttctatt tcatggatga atcagcaatc gatcattgaa
     6541 caaagacagt tgcttgctga aaaagaactg gttaacttag atcagcagat gaaagaagat
     6601 gaaactttat tagcagattc gttacaaaaa cgtctggcat atgaatatcc ttatcaagct
     6661 tcttcacaaa ccacaagtta tcaatctgta tctgaaatca aacgattatt cgaagatcca
     6721 gatgatacac aagaatcacg tttgactctg gaaagcagtc agaacaaagc agcatcccgt
     6781 caatttcgtt atacgcaaga acaactagca gaaccaaaat ttttgcaaaa agatcggcaa
     6841 gtgtcagcag ctgctgttgg aactgctacg catgctttgt tacaattact gccattagag
     6901 atgccaacta cagagagtat ccatcaaaaa ttacaggaat tggtaaggaa acggttagta
     6961 gacgaaaaag tcgcgaaaaa agtagatgtt tcttctatta tatggttctt tcaaacagaa
     7021 cttggtcaac agctgattgc caacaaagaa aacgtaaaac gagaacaacc tttttccatg
     7081 ctgcttcctg ctgacgaagt atttcaagac tatccaaatc aagaagatga actattaatc
     7141 cacggaatcg tcgatggata cctagaagaa aaagatcatc tcaatatcta tgattttaaa
     7201 acagatttta ttttacatcc ggatgatcca gcagaaatag atgcaatcgt ccaaaaatat
     7261 caaggacagc taagacttta ccaacaagca atgtcagaag ctttgaataa gccagtagaa
     7321 aatgttttcc taattttgct tcgagtgaaa caaattataa acattaacaa ataattataa
     7381 ttgagcaaac ttgacttatg ttttgtaagg attatgctat actctgggaa gaggtgagta
     7441 tgatggagct aacacagaca gaattcgttt tatgccctaa atttgaaaaa acattctcta
     7501 ttttaggaaa aaaatggaac ggattgatta tcgatgtact actcgaaaac ggtccgcaac
     7561 gctttgttga gcttgcggca aaaatcccga tggtcagtga tcgagtatta gtcgaacgct
     7621 tgaaggaatt agaaaaagaa ggaatagtca ctcgaaactt tgcctgtagc gaaaagagcc
     7681 gttcagaata tctcttgact gaaaaaggag cagacttgca aaaagcgatg cgtcaaatac
     7741 aagtttggag cgaaaaatgg atcacagagg aagaatgcgg ttgattttgt aaaaaaaatc
     7801 ttgtaaacta tagtccatac gaataagtaa agaacgatga cgaaaaagag tagtttggct
     7861 aaaccagttc agggagatct gtcgcagact gaaagcagat cctgtgtttg aaaaatgaag
     7921 ttcactttcc gagttcgtct gataaaatca gaccggcaat tgtcgttaac gaagtaagag
     7981 tgcggtgtaa gtcatttatc cgcaaattag gatggtaacg cgaagacctc gttccttaac
     8041 aggaatgagg tctttttgtt ttgtatttgg aatttaaagg aggaacaagc acatgtctct
     8101 acaagcacaa ttagagagct tgaaagaagc aacgctcgtc aaaatcacag cggcacaaga
     8161 tttaaatgaa ttaaatcaaa tacgtgttga aacgttagga aaaaaaggac cgatcacaga
     8221 agtattacgt gggatgaaag atctaagtcc agaagagcga ccaaaagtcg gaagtttcgc
     8281 aaatgagatc cgagatcttt tgacagaaga gatcgagcga aaaaaacaag tattagaaga
     8341 agaagcgtta aatgcagctt tggcaaaaga aacaatagac gtgacactac ctggtagaaa
     8401 agtaaaacat ggtacacgtc atatcgtgac acaaatcatg gaagaaattg aagatatttt
     8461 tgtgggtatg ggctatcaga tcgtagaagg atatgaagta gaatctgatc attacaattt
     8521 cgaacggatg aatctgccaa aagatcatcc agcaagggac atgcaagata ctttctatat
     8581 ttcagacgag atattgattc ggacacatac ttcaccagtc caagcacgga caatggaaaa
     8641 acatgatttc tctaagggtg ctctacgaat gatctcgcca gggaaagtct tccgcagaga
     8701 tacagatgat gcgacccaca gtcatcagtt ccatcaaatt gaagggctag ttgttgataa
     8761 aaacatcaca atgggcgacc ttaaaggaac attagaagtc gtaatgaaaa aaatgtttgg
     8821 ggaagatcgt gaaattcgtt tgcgtccaag ttatttccca tttacggaac catcagtaga
     8881 agtagatgtc agctgtttca aatgtggtgg tgccggttgt aacgtatgta aatacactgg
     8941 atggatcgaa attttaggag ctggcatggt acacccgaat gtgttgaaga tgtcaggaat
     9001 cgatccagaa gaatattcag gctttgcttt cggccttgga ccagaccgcg tagctatgtt
     9061 gcggtatgga gtgaatgata tccgtaattt ctatcaaaat gacttacgtt tcttaagtca
     9121 gttcaagggg gagtaagaaa atgttagttt catataaatg gctcaatcgt tatgtagatt
     9181 tatcgaatgt aacaccaaaa gaattagcgg ataagatgtc tgtgacagga atcgaagtag
     9241 agggagtaac ggttccagaa gaaggcttga aaaaaatcgt tgtcggtgaa gtcaaggaat
     9301 gcgtaccaca tccagattct gatcaccttt ctatttgtca agtcgatatt ggcgaagacg
     9361 aactgtctca aatcgtttgt ggagcgccta atgtgaaggt tggaatcaaa gtgattgttg
     9421 cacttcctaa ctcacgtatc gctggtaatg taaaaatcaa aaaaggaaaa atgcgtggcc
     9481 aagtatcaaa tggcatgatt tgctcattac aagaaattgg ctattcagat agtgttatac
     9541 cgaaagaata cgctgaaggt atctattata tgccacaaga agctgtaaat ggcgatccag
     9601 tttttcctta tttggatatg gatgattcga ttattgaatt atctatcaca ccgaatcgtg
     9661 cggatgcatt gtcaatgcgt ggcgtagctt atgaagttgg ggcaatttat cgtcagactc
     9721 ctacattcga tgacgaaaag ctggtagaat cctcacgtcc tgctagcgat aaaattcaag
     9781 tggaagttga agataaagaa gcagttcctg cttatcaaat cagaatcatt gaaggagtaa
     9841 gaatccagcc aagtcctcaa tggctgcaaa atcttttgat gaacgaaggc attcgtccaa
     9901 tcaacaatgt agtagatgtg acgaactata ttttgctttt attcggacaa ccactacatg
     9961 catttgacta cgacaaattg aaatcaaaag agatctttgt ccgccgtgca aaagaaaatg
    10021 aaacattagt aacactagat ggagaatctc gggaattgac aactgacaat ctcgtcatta
    10081 ctaacggcaa agaaccagtt gctttagctg gtgtcatggg agggttagac tcagagatca
    10141 cgaatgaaac aacaacagtt gcactagaat ctgctttatt tgatccaatt tcgattcgtc
    10201 gtacagcaaa acaatttaat ttgcgtagtg aatcctctgc acgttttgaa aaaggaatca
    10261 accatgcgac tgttggagaa gccagtgagg ttgctgcagc aatgatcgtt tctcttgcgg
    10321 gtggggaagt tctacaaggc gcggtcaaag gctcagagat caaagcagaa gacgtatcag
    10381 tgaagatttc acttgaacgc atcaatcaat atttaggaac atctcttgtc gttaaagaag
    10441 taaatgagat ttttgaagca cttggattta gctatgaaga aaaggagtat gtttattccg
    10501 tagtagtacc accaagacgc tgggatatcc aaatcgaagc agatattatc gaagaagttg
    10561 ctcgtattta tggatacgac cagttgccat ctacacttcc aagtggagaa acggttgctg
    10621 gaagcttgtc aaaagaacaa gaaactactc gtaaaattcg tacaatcttt gaaggaagcg
    10681 gattgagcga agcaatcagt tatgcattga caacagaaga aaaatcaaga cagtttgtta
    10741 taaatgaaag taatattaca cgtttggact ggccaatgag cgaagaacgt tctgtattac
    10801 gaatgaattt gatcagcgga ctattagatg atatcagtta taacactgcc cgaaaaaata
    10861 cagagattgc tttatatgaa attggtcggg tgttcttcca agaaaatgat ccacgtacac
    10921 atctgccgca agaagtaaaa catgcagcaa tcgctatttc tggtatttgg gaagaaaaag
    10981 attggcagac gaaagctcaa aatgtagact ttttcaccat caagggtctt ttagaaaact
    11041 tatttgaaca actaggcatc actgaagatg tccattatca aacagtgaca gaaatgaagg
    11101 aaatgcatcc tggaagaact gctgcgattt accttggcga aaaatttatt ggttttgtcg
    11161 gtcaagtgca tccaacgatt acaaaaagtt atgatattcc agaaacatat gttgctgagt
    11221 ttgatttatc tgctatagtg gaagctgctc aaaaagggat cgttttcgaa gcagtgacaa
    11281 aattcccagc agtcagccgg gacatcgctt tgcttgtaaa agaaactgtg acaaatcagg
    11341 aattaacaga agttatccgt tctgctgccg gaagattttt aacagatatc caattatttg
    11401 atgtctatca aggagaaaac attgaaaaag gacataaatc catggcgtat agcctgacat
    11461 tcgtcaaccc agaagcaaca ttgacagatg aagaaatcaa taaagcaatg gaaaaagtcg
    11521 aaaaagcgtt ggtagaaaac ctagaggcaa gtattcgcta agaaggaaaa tatagtaagt
    11581 aacgatcaag tagcttttga agaccataca tttgaaaaaa agatgtgaca caacttcgtc
    11641 acatctcttt ttttgatttt aaaaaaggaa tagcacagag acgagataaa aaaacaggcc
    11701 tttacagcct gcttttttag agtaaatgaa ttaatggaag taattgattc ccattgctga
    11761 ttttacttca gaaagtgttt gtgcagctac tttagcagct tcttcactac ctttacgtaa
    11821 gatttccata acagctgaag gatctttttc caattcttct cggcgcgcac ggatcggtgc
    11881 aaattcagct tccaatacat caatgaggta tcgtttgatc tttacatcac caagtccgcc
    11941 gcggcggtaa tgagctttca tttcttcaat tgcttcttta tctgttccga atacatccaa
    12001 gtaggtgaag accatattcc cttctacttg tccaggatct tgtacatgga tgtggtttgg
    12061 atcagtatac atactcatga cttttttctg taagacatcg gcaggatcgg agatataaat
    12121 accgtttcct aacgatttac tcattttgcc atttccatcg attccaggta atctacccat
    12181 tccttttggt gggaatacgc cttttggttc caccaagact tcaccatatg tatgattgaa
    12241 gctttgtacg atttcctgtg tctgctctag cattggtttt tgatcctcac caacaggtac
    12301 aagattcgct ttgaatgcag tgatatctgc tgcttgagaa acaggataga taaagaatcc
    12361 tgtaggcacg ctttcaccga attttttttg ttcgatttct gttttgacag tcggattgcg
    12421 ccgaacgcgt cctacgctga ctaagttcaa ataatacatg gttaattcag ctaattccgg
    12481 aatttgtgat tggataaata aagtagattt tgcaggatct aatcccacgg ctaaatagtc
    12541 taaagcaact tgcaagacat ttgatgaaac cttctcagga tttttagcat tatctgtcag
    12601 tgcttgcatg tcagcaatca tgacaaacag ttggttgtct tcatctgcct gcatagccac
    12661 tcgggttttt agagaaccga cgtaatgccc taaatgcagc ttcccagttg gtcgatctcc
    12721 cgttaaaata atatttttca aaaaagacac ctctttacga tttgtaagtc tgagcactta
    12781 atatcttacc gtttttcctg aaaagaaaca acagttccta aggaaaaaac aaggaaagag
    12841 gataaaataa ctaatagatt agaaatggaa gaaatattta tgggagaata aacgttctaa
    12901 gaaactttac aaattactaa actatcagat ataatgtatt ttattataca tctttaaaag
    12961 aaatcttact aaataataat gggagtgtaa ttctatggca atgattaaat ttgatcacgt
    13021 ggataaatat tatggcaagt tccacgcatt aaaaaatatc aatctcgaat tcgagaaagg
    13081 agaagttgtc gttgtcattg gtccttctgg ttcaggaaag agcacgatgc ttcgctgtat
    13141 caatggatta gagacgatct cttcaggtaa gctgctgatt aatgatactg acttgcatga
    13201 taaaaagaca aaactgacag aagtccgaaa aaacatcggc atggttttcc aacatttcaa
    13261 tttgtaccca aataaaactg ttctggaaaa catcaaatta gcaccaatca aagtgttgaa
    13321 gcaagaccag gcaactgcag tcaaaaacgc ggagaaattt ttgaaaactg tcaatatgtt
    13381 agataagaaa gattcttatc cttccatgtt atccggggga caacagcaac gggtggcaat
    13441 cgccagaggt ttagcgatga atcctgagat gctgctgttt gatgaaccta cttctgcttt
    13501 ggatccggaa atgattggtg atgtattaga cgttatgaaa aaattggctc atgacggtat
    13561 gtcaatgatt gtcgtgacac atgaaatggg ctttgccaaa gaagtggcgg atcgtgttat
    13621 cttcatggct gatggagaag tattggaaga cagcagaaat gtgagagact ttttcgaaaa
    13681 tccaaatgaa gtccgagcac agcaatttat cagtaaagtt atcaatcatt gataggagga
    13741 aaaagatgcg caggttaaaa aaaatacgcc tgattttcct tttaggcatt gcttttcttg
    13801 cactagccgg atgtaaagga aatgggttgg caaatgaaga tattttagag cgaagcaaat
    13861 ctaccaatga aatcatctgg ggtgtcaagt atgatactcg attatttgga atgatggata
    13921 ttgagagcag aactgtacaa ggatttgatg tagatattgc aaaagcaatc actaaaaaga
    13981 tccttggtga caatggaaaa acagaatttg ttgaagtcac ttctaaaact cggattccat
    14041 tattgaaaaa cggaaatatc gatgcaatta ttgcaacgat gacgatcaca gatgaacgaa
    14101 aaaaacaggt tgacttttca gatgtctatt tcgatgctgg ccaagctttg ttggtaaaga
    14161 aaggaagcca aatcaaaagt gtagatgatt tgaatgcttc tacaacggtt ctagcagtca
    14221 aaggttctac atcggctgcg aatatccgac agcatgcacc agatgcaaaa attttagaat
    14281 tagaaaacta tgcagaagca tttactgctt tacaatctgg tcagggagac gcgatgacta
    14341 cggataatgc gattttgtta gggattgcag atgaaaatcc agagtatgag ttagttggtg
    14401 gaacgtttac taatgagcct tacgggattg cgatcaacaa agggcaagaa aacttcttga
    14461 aagctgttaa ccaagcgcta gaggaaatgc atgcagatgg cacatacgat aaaatctatc
    14521 aaaaatggtt tccaaatgaa actgaaggaa aagtagagta agggggagag gcaatgagcg
    14581 aattgataca aacttatgga ccaatctttg tcgacgggtt caagatcaca ttgttttcga
    14641 gtgtcttagc acttgtgttt agtttgatta tcggaacgtt aatggcaatc ttccaattat
    14701 caaagaataa agtagtgagc gccttagcaa aggcatatgt agaatttttt agaaacattc
    14761 cattattaat catcgtgatg tttttttatg ttgtcgtacc gatttattgg tttaatctag
    14821 atggattcca agcaggtacg attggattaa cgatctatac atctgctttt attgctgaaa
    14881 cagtacgttc agggattcaa accgtaccaa aaggtcaaac agaagcagga ctatcttctg
    14941 gttttaccta ttcagaaatc atgcgttata ttgttttacc gcaagcattc aaaatcgtta
    15001 tcccgccatt aggcaaccaa tttatcaacc tcgtaaaaaa ctcatctatt ttagcaatcg
    15061 tagctggttt agatttgatg tatcaaggag acttgatcgc tagtgaaaca ttcaatacct
    15121 tcgatacgta tatcatcgtt gggttatttt acttagttat cacgctaccg ttatcttact
    15181 taatggttta tcttgagaaa aaatgggcgg ttcgttcata gaaggagggg aaatacatgg
    15241 attttagcgg cgctttttca tggatgaata tccgtttttt attggaagga ttaaaggtta
    15301 ctgttgaagt ttcattgttt tcgatcattt ttagtttctt gatcggtggt ctgacaggtg
    15361 tgattcgttt tgcgaatatc cctttctttt ccaaaatcct tggtgtagtg attgatatta
    15421 tacggaatct tccattatta ttgatcattt tcttcactta ctttgcctta cctcaaatcg
    15481 gcattcagat gaacatcttc tggtccgctg tcgcagcatt aacgattttt gagtcagcta
    15541 tgttgtcgga aattttccga gctggactca atgctgttcc aaaaggacag atggaagcag
    15601 gattatcaac gggactgact tatgtagaat cgatgcgaac gatcgtgtta ccgcaagcat
    15661 ttaaatcaat gatcccggca attgtgagcc aattgatttc actgatcaaa gatacttcat
    15721 tagcagtaat catttcttta cctgaacttt ctcatcaggc acgtattatt tatggacaga
    15781 acaccaatta tgtgcttccg atgttcatca tcatgacact tatgtacttc gtggtatgtt
    15841 acgcgctttc tctgctttcc ggttacttgg aaaagagacg gtacagttat taatagatga
    15901 ttcttttagc ggttgtggca aactaaagtc acaaccgctt ttcctctttc cattcgaaaa
    15961 aaagagattt cgattgtttt aagcaagtaa gagcacttga aaattcacgc taaaaagtac
    16021 acaataggga ggatagaatt tattgaaaaa aaggatgata cgattgacag ataatcgccc
    16081 tatcggattt attgattcag gtgtcggcgg cttgactgta gtaaaagaag ccctgaaaca
    16141 attaccgaat gaaaatattt tatttgtagg agacacagca cgctgcccat atggccctag
    16201 acccgcggaa caggtaatcc agtatacttg ggaaatgacg gattatctgg tggagcaagg
    16261 aatcaagatg ctggtgatcg cctgcaatac cgcaactgcg gtggctttag aagaaatcaa
    16321 agctgctctt tctattccag tcatcggtgt gatccttccc ggtactagag cggcagtaaa
    16381 aaaaacacaa aataaacaag ttggcattat cggtacgatt ggtacggtaa aaagtcaagc
    16441 ttatgaaaaa gcactgaaag agaaagtacc agaattgact gtgacaagtc ttgcttgtcc
    16501 caaatttgtt tcagttgtcg aaagtaatga ataccattca tcggtggcga aaaaaattgt
    16561 ggcagaaaca ttagctcctt taaccactaa aaaaatcgat acattgattt tgggatgcac
    16621 ccattatcca ttattacgcc ccatcattca aaatgtaatg ggagaaaatg ttcaactgat
    16681 cgattctgga gcagaaacag taggtgaagt atctatgctg ttagattatt tcaatttgag
    16741 caattcaccg caaaatggtc ggacattatg ccagttttat acaactggct ctgccaaact
    16801 tttcgaggaa atagctgaag actggcttgg aatcggacac ttaaatgtag aacatatcga
    16861 attgggagga aaataaggat gcgtcatgac gggagaaaag cccaagagct tcgcaaactg
    16921 gagcttcaaa caaatgtttt caaatatcca gaaggatcgg tagtgatccg ttttggtgat
    16981 acaacagtta tttgttctgc aacaatagaa aattctgttc cccctttttt gagagaaaca
    17041 ggaacaggat gggtgacagc agaatatagt atgctgcctc gagcaacgag tacgagaaat
    17101 cgtagagaaa gcagcaaagg gaaactttct ggtagaacaa tggaaatcca gcgtcttatt
    17161 ggtcgttcct tgcgtgcagt agtagacttg gaaaaattag gagaacgtag tatcatcgta
    17221 gactgcgatg tgatccaagc ggatggcggt actagaacag caagtatcac tggggcattt
    17281 gtggcattga aattagccat tgaaaaactt ttaagagaaa aagaattgtc agaagatcca
    17341 ataaaagaac atcttgctgc agttagcgta ggaatcttac cagatgggac ttgcgtcaca
    17401 gatttggatt accaagaaga ttccgcagca cttgtagaca tgaatcttgt aatgacagaa
    17461 tctggaaaat tcgtggagat tcaaggaact ggagaagaag caacgtttga tggtgaacag
    17521 ttgaatgaaa tgctcttttt tggaaaaaac gcgattgaag atttgatcaa agagcaaaaa
    17581 catgctttgc taacagagtt tgctcaaaat gatgagcgga tagaagaaac aaaaacaatc
    17641 atcattgcga caagaaatcc aggaaaagca gaagagtttc gaaatatgtt caaagaagcg
    17701 ggatatcatg taaaaacatt acttgactat cctgaattgc cagatgtgga agaaactggc
    17761 agcacatttg aagaaaatgc ccgattaaaa gcagaaacga ttgcacaatt gcttgatcag
    17821 ccagtcttag cagatgattc aggtttgaaa gtggatgctt taggcggtat gccaggaatc
    17881 tattctgcac gttttgcagg ggaacaaaaa agtgatgcgg ggaataacgc caaattatta
    17941 tatgaattga cagatgtgcc ggatgaaaaa agaacagcac aattccattg tacgttagta
    18001 ttcgcggcac ccaaaaaaga cagccttgta gtagaagcgg aatggcccgg tcgagtagcc
    18061 cgtataccat ctggcgaaaa tggctttggg tatgatcctt tatttatccc agaaggtaag
    18121 aaacaaacag cagcagaatt atcgagcgaa gaaaaaaaca agatcagtca tcgggctcaa
    18181 gcaatgaaaa aattgagtgc cgagtggaaa caatggctgg aaggagagag atagatgcgg
    18241 tatttagtag taagtgataa tcatggagac agagatatcc tcgttgactt agtagatcgt
    18301 tatcgttctg aagtggatat ctttttccat tgtggtgatt cggaattaga agcttctgac
    18361 ccattatggg atacattcca agtggttcaa ggtaattgtg attatggacc aggattcgaa
    18421 cagaaaaaag tgatccaaac tggtcaggat acagtattca tgactcacgg tcatttatcg
    18481 aatgtccgat ttggtttgac acagttagca atcgaagctc gagctgcaac tgccgatatg
    18541 gtgttttttg gtcataccca tcaaataggc tgtgagatgg atcagaaaat cctgtttttg
    18601 aatcctggta gcatttctca gcctagaggc ccgatccaaa tcccatcata tgctcttatc
    18661 gaaagtttag atgaccaaat ttctgttcaa tactataacc gtgcccatca ggcagttgaa
    18721 gagttggcct tttctttttc gagagagcgc tgaataaaaa aactctaatt tttaataaaa
    18781 ataagcttta ttttatgaat tttgcatccg gtttcggtaa aatagtagtg ttgaatgaaa
    18841 tagaaatgga ggccatcaga tgattggacc tatagtaaga gaaatgctgc tggaaaatca
    18901 ggaaacgttt ttgattcctg cagaaaatgt ggcaaatgtt atgtatcatc atccattgtc
    18961 ccatggttta cttgtgttat cgaaggtcgg ttattccaaa attcctgtat taggaaaaga
    19021 tgatcacttt gtaggactag taagtttatc agatgtggtc aataaaatga ttgacttgca
    19081 aacaatcagc atggacccgt tagaaggact aacggttgct gatgtgacag aaacagatgt
    19141 tccgacaatt gatgaaaatt gggaactaga agaagtactc catttgttag tcgattcgcc
    19201 ttttttaccg gttgtcacag aagatcggat tttcaaagga atcattacaa gaaaagagct
    19261 gcttaaagcc gtaaacttca tggtgcatga attagaacgt cgtaatatat tgtctccgaa
    19321 aatcgaagaa ttaaaagaac aaatcgatat cgtgagctaa agaacgagaa actaaaagat
    19381 aatcaaaaaa taagtagaaa aaacagcgta ccagttttga ctttatcttg aaaaagaggc
    19441 tgggacaaaa gc
//
LOCUS       DACEKQ010000056        19403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEKQ010000056 DACEKQ010000000
VERSION     DACEKQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.059"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..364
                     /locus_tag="IW671_001764"
     CDS             170..364
                     /locus_tag="IW671_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898749.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="IW671_001765"
     CDS             633..932
                     /locus_tag="IW671_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898750.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="IW671_001766"
     CDS             complement(1029..1463)
                     /locus_tag="IW671_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0898751.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="IW671_001767"
     CDS             complement(1695..2405)
                     /locus_tag="IW671_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0898752.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="IW671_001768"
     CDS             complement(2395..3255)
                     /locus_tag="IW671_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0898753.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="IW671_001769"
     CDS             complement(3260..3898)
                     /locus_tag="IW671_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0898754.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="IW671_001770"
     CDS             complement(3913..4212)
                     /locus_tag="IW671_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0898755.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="IW671_001771"
     CDS             complement(4255..5739)
                     /locus_tag="IW671_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0898756.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKATCLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="IW671_001772"
     CDS             complement(5759..6220)
                     /locus_tag="IW671_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0898757.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IW671_001773"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IW671_001773"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0898758.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="IW671_001774"
     CDS             7523..8293
                     /locus_tag="IW671_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0898759.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="IW671_001775"
     CDS             complement(8566..9393)
                     /locus_tag="IW671_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0898760.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="IW671_001776"
     CDS             9961..11196
                     /locus_tag="IW671_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0898761.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="IW671_001777"
     CDS             complement(12195..12455)
                     /locus_tag="IW671_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0898762.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="IW671_001778"
     CDS             complement(12482..13783)
                     /locus_tag="IW671_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0898763.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14075..14533)
                     /locus_tag="IW671_001779"
     CDS             complement(14075..14533)
                     /locus_tag="IW671_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0898764.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14546..15871)
                     /locus_tag="IW671_001780"
     CDS             complement(14546..15871)
                     /locus_tag="IW671_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0898765.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15888..17300)
                     /locus_tag="IW671_001781"
     CDS             complement(15888..17300)
                     /locus_tag="IW671_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898766.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17317..18081)
                     /locus_tag="IW671_001782"
     CDS             complement(17317..18081)
                     /locus_tag="IW671_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0898767.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18185..19060)
                     /locus_tag="IW671_001783"
     CDS             complement(18185..19060)
                     /locus_tag="IW671_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898768.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19315..19399)
                     /locus_tag="IW671_001784"
     tRNA            complement(19315..19399)
                     /locus_tag="IW671_001784"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19363..19365),aa:Leu,seq:caa)
BASE COUNT         6170 a         3989 c         2987 g         6257 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagtg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
    13861 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
    13921 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
    13981 aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat
    14041 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
    14101 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
    14161 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
    14221 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
    14281 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
    14341 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
    14401 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
    14461 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
    14521 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
    14581 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
    14641 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
    14701 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
    14761 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
    14821 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
    14881 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
    14941 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
    15001 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
    15061 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
    15121 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
    15181 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
    15241 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
    15301 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
    15361 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
    15421 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
    15481 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
    15541 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
    15601 atcctccaac acgaatttgt gagaaaacaa aatcgcgtcc tgtaaaagca agctttggat
    15661 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
    15721 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
    15781 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
    15841 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
    15901 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
    15961 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
    16021 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
    16081 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
    16141 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
    16201 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
    16261 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
    16321 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
    16381 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
    16441 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
    16501 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
    16561 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
    16621 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
    16681 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
    16741 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
    16801 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
    16861 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
    16921 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
    16981 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
    17041 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
    17101 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
    17161 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
    17221 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
    17281 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
    17341 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
    17401 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
    17461 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
    17521 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
    17581 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
    17641 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
    17701 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
    17761 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
    17821 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
    17881 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
    17941 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
    18001 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
    18061 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
    18121 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
    18181 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
    18241 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
    18301 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
    18361 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
    18421 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
    18481 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
    18541 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
    18601 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
    18661 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
    18721 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
    18781 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
    18841 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
    18901 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
    18961 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
    19021 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
    19081 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
    19141 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
    19201 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
    19261 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
    19321 cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg
    19381 ccaattccgc cacgccggca aat
//
LOCUS       DACEKQ010000057        18792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEKQ010000057 DACEKQ010000000
VERSION     DACEKQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.061"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..160)
                     /locus_tag="IW671_001785"
     CDS             complement(<1..160)
                     /locus_tag="IW671_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0898769.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
     gene            426..770
                     /locus_tag="IW671_001786"
     CDS             426..770
                     /locus_tag="IW671_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0898770.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            771..1190
                     /locus_tag="IW671_001787"
     CDS             771..1190
                     /locus_tag="IW671_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0898771.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1282..1632)
                     /locus_tag="IW671_001788"
     CDS             complement(1282..1632)
                     /locus_tag="IW671_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898772.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1598..2929)
                     /locus_tag="IW671_001789"
     CDS             complement(1598..2929)
                     /locus_tag="IW671_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0898773.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3185..3751
                     /locus_tag="IW671_001790"
     CDS             3185..3751
                     /locus_tag="IW671_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0898774.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3896..3983
                     /locus_tag="IW671_001791"
     tRNA            3896..3983
                     /locus_tag="IW671_001791"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3928..3930,aa:Ser,seq:cga)
     gene            4317..5291
                     /locus_tag="IW671_001792"
     CDS             4317..5291
                     /locus_tag="IW671_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0898775.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5616..7511
                     /gene="asnB"
                     /locus_tag="IW671_001793"
     CDS             5616..7511
                     /gene="asnB"
                     /locus_tag="IW671_001793"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0898776.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7706..8152)
                     /locus_tag="IW671_001794"
     CDS             complement(7706..8152)
                     /locus_tag="IW671_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0898777.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8380..8529
                     /gene="rpmG"
                     /locus_tag="IW671_001795"
     CDS             8380..8529
                     /gene="rpmG"
                     /locus_tag="IW671_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0898778.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8617..9135
                     /locus_tag="IW671_001796"
     CDS             8617..9135
                     /locus_tag="IW671_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0898779.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9212..10168)
                     /locus_tag="IW671_001797"
     CDS             complement(9212..10168)
                     /locus_tag="IW671_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0898780.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10334..11140
                     /locus_tag="IW671_001798"
     CDS             10334..11140
                     /locus_tag="IW671_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898781.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11137..12126
                     /locus_tag="IW671_001799"
     CDS             11137..12126
                     /locus_tag="IW671_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0898782.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12123..13127
                     /locus_tag="IW671_001800"
     CDS             12123..13127
                     /locus_tag="IW671_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0898783.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13259..13801
                     /locus_tag="IW671_001801"
     CDS             13259..13801
                     /locus_tag="IW671_001801"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0898784.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13821..14519
                     /locus_tag="IW671_001802"
     CDS             13821..14519
                     /locus_tag="IW671_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0898785.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14540..14752
                     /locus_tag="IW671_001803"
     CDS             14540..14752
                     /locus_tag="IW671_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0898786.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14752..15714
                     /locus_tag="IW671_001804"
     CDS             14752..15714
                     /locus_tag="IW671_001804"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0898787.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15732..16127
                     /locus_tag="IW671_001805"
     CDS             15732..16127
                     /locus_tag="IW671_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0898788.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16290..16655
                     /locus_tag="IW671_001806"
     CDS             16290..16655
                     /locus_tag="IW671_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0898789.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16652..18463
                     /locus_tag="IW671_001807"
     CDS             16652..18463
                     /locus_tag="IW671_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0898790.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18525..18692)
                     /locus_tag="IW671_001808"
     CDS             complement(18525..18692)
                     /locus_tag="IW671_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0898791.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6030 a         3177 c         3869 g         5716 t
ORIGIN      
        1 attctaaggc tgattttcct tgcttgaaga acagatatac tttatggatc gtttgtcttg
       61 aacgattcaa gcaatgggca gttttagcaa cagattgatt cattttgaaa taagactcta
      121 ttattacaag ttcatccgtt gtaagatggg tataggtcat ttgtactcac tccttataat
      181 tctttggtcg gaactatttt gagtgtagca caaatgactt ttttagttgt ctagcttaat
      241 tttacaatcg gcgaataaaa aaagcgttag caactatgct taaaaagcat atcgactgaa
      301 taaactaagc attggacagg ctgaaagact acatttaaaa tgaaatgcga agaaacaata
      361 gaaaataaag ttgccttaga accatagttg aatcacctaa cagtcgtgaa gggagaataa
      421 gagaaatggc agaaaaacaa accgcaggac gagatcaatt aggagatttt gcaccaaaat
      481 ttgcagaatt gaatgatgat gttttatttg gacaagtatg gtccagagaa gaattatctg
      541 ctcatcagcg tagtatcatc accatatctg cattaatttc aggaggaaat tttgaacaat
      601 tgccagctca tctgaagatt ggcaagcaaa atggcattac tagtgaagaa atagcagaaa
      661 tcattaccca tttagctttt tatgtaggat ggccaaaagc ttggtcagct tttagtttag
      721 caaaagaaat ctatttagaa gaaaatagcg aagataagaa agaggcgtaa atgacgacaa
      781 aacacgaaga aatacacaat ggcgtgattt ttccagcagg tgaaaaaaac gaagcttacg
      841 ctcaagtatt taccgggcaa agctatctga atatgttagt gaatgatccc gaagtttctg
      901 ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa tcactggcat gtccataaag
      961 acggctatca aatcttactt gtgaccggag gagaaggctg gtatcaggaa gaaggaaagc
     1021 cagcaagacg attaatggct ggagatgtcg tcgtaacgca caaagggatt aaacattggc
     1081 atggtgcgac aaaagacagt tggtttgaac atttggcgat cactgctggt aattcagagt
     1141 ttttagaacc tgtaacagat gaagtgtatg aaggattgga aaatcaataa tgtcaagctt
     1201 gaacagctcc tgaaagtaga ttttaatcga actttcagga gctgcttttt tgattcagtc
     1261 ttttataaat agaaaatagg gttaataata ggaatgagaa gcacgtcgaa agatcttaat
     1321 attgataatc ggctgggcat acatgatttc cacgtcatca atcaaactac ctaacagatg
     1381 ataatcttcc cgatcatgtc ggggattacc taatagttct tgataataga tttccatact
     1441 agggtcccga ggattgttca gcaaatcagc aagtacatct aaatcttctg gctgagtttc
     1501 tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca aattctactc caatacgtat
     1561 ttcagttatt tcatgggaat ataaataagt aagaagctca tcgatgattc tttttgataa
     1621 ttctttttcc aagtgtcttt ttttcctttc tgacagcggt aaagttatct agtatcggta
     1681 caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt atatagattt agtcctccgt
     1741 gcaaagtgct gacgttatgg acaagagcaa tatgtagtac accagctatt acaccgtaaa
     1801 aactgccgta aaaaccgctt atgggcgcga gacctgtccc aaacacagct gcaacaagca
     1861 tgccagtact ggtgatctca tgaaaagttc caaacaaaga agcaataaat attccgacta
     1921 gtatcggaaa actgtttttt aaatgacaac caaaagcact gaaacccacc gcagataaaa
     1981 tagcaccgat gacaggtcca tttaactgac cgccaaccag caaaacataa gaagacagca
     2041 tcagaccaac taagcctatg ttgatcaaag tagcaccaat accagaatta gcaatgaaat
     2101 cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat ccccaaaagc cgaaaggaat
     2161 tataatagaa accaattccg actgtcagcg aaaaaaagcc aaaaataatc caaatgagag
     2221 gaaagtgata ttcattgaaa accaatgtat tttcgacgat tgaatagctg aataacctca
     2281 aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa tccaatatta taaagtgtga
     2341 atccacgatg gaaaactaaa gtttgagcag ctaagggcgg aagaagtaga ccaattgcta
     2401 tgccagctaa attccccaat aaaataccta tcagtaaagg aaaacgcata ccaaaagtga
     2461 tataactaac gaccggtgat aatgcgctac caaacagtcc gatcatgatg tactgagaaa
     2521 aaggtttatt gacaaataca gcgtatagat acacacctaa aatgattgag atagaattat
     2581 aaaggttttt gccaaaaaaa gagaaaccgg taactgttag aatcgaagca aataagggac
     2641 cgtttaaaat caccttgtgc cgataagcta aaagcacaga aagtaaggtc aaaatcccta
     2701 catttatgaa tgcgcttccg aaaccgccta aagcaaaata atctgtcaaa agattgcttg
     2761 gcgaggtaag aatagttagc aatccactcc aattatcagt aggagtatta gaaagcaagc
     2821 caataaaaag aaaaaaaaca cctgtaaaac caagataaaa atactttctt ttctgtgaaa
     2881 cgattcgttt ttgaggatga atataaataa cgtcagtttc gttttccatt acacaccttc
     2941 tttttcattt tctttaacta taacttgaaa tgaaagattt ggacaactat catttaggtc
     3001 aaaactaaaa atgtaaggga acccattgaa tttaaccaaa tttttttaat tttgcaaaaa
     3061 aaagttaaag aagactgaaa aaaaatttaa aatataacgt tttgagtaaa atgtttgcaa
     3121 aaaatgctaa aaatagggta caataaaaca acatatactg cagtaaagaa gtgggggagt
     3181 tttaatgaga agaaaagtct ataccaagga tcaaattcta aaagctgctt atgatgttgt
     3241 tgcaaaggaa gggtttaaag gatttactgc gagaaatatt gctaaaaaga tggggatttc
     3301 gacacagccg atttatctgg aattcaaaaa catggaagat ttaaaaaata cgttgctgga
     3361 ttctatcttt gaacaactaa aagaagatgt tttccctatt gaacgtactg gtgataaaat
     3421 cattgatctc ggtttgaact atatctactt tgcaaaaaag catcatagtc tttacatggc
     3481 attgtacatt gatgaacatg gggacggaaa gaaaatgcat gatttttcat acaattattt
     3541 ccgtgaaata gttaaagaag atgaaagata caatcaatta tcggatgaat atgtcgatgc
     3601 tctgcatact ggtacatggg tagcagtgac tggcttagcg tcattgatgt catcaaacat
     3661 catgcatcca acagaagaac aaatcattgc actgatcaaa cggacaattg actcgatttt
     3721 agcattagat aatcctgaaa taatcagata gtgttctatc agtatgaatt tgatcggtaa
     3781 gtccgttggc tgcaaactgt caatggactt ttttgctaaa aaaagcgatt tctgttattt
     3841 atctattaga aataggcttg acatttcgtg aaaaaagttt aaaaatagta cttacggaag
     3901 gttggcagag tggtaatgca ccggactcga aatccggcga accggctaat accggcgcgc
     3961 aggttcaaat cctgtacctt ccttatatca acgattagag gttgtgaaga agctgtcctg
     4021 catcaagtaa taagaacggc caaacaaaaa tagcttttcc tattttttcg tttgattggg
     4081 cttattaccg cagatgcaag cttttgaaca ccgtttgttc ggaataaggg gctgtgacaa
     4141 gacttttgtc acagcccctt attgttgtac ttttaaccta caatattttt tgattccccc
     4201 tgagtactat tttttataaa tgctagtaaa tcaatcttct ttggcctttt tttgataaaa
     4261 aaaatactag atgtaagata aaagtgtaaa aacgatttgt tttaggacgg gaaacgatgt
     4321 gtaccgcaat cacttatgca acgaaagacc attattttgg aaggaatttt gattatgaga
     4381 tgtcttataa tgaagtggtc actattactc caagaaatta tcgtttcgat tttagaaagg
     4441 taaaaaattt agataaacat tatgcaatga taggaatagc agctggggta tccaactatc
     4501 cactctatta tgaggctaca aatgaaaaag ggttgagtat ggcaggactg aattttcctg
     4561 gcaatgctga ttataaagaa ttacaagaag gaaaagataa cgttgcacca tttgagttta
     4621 ttccatggat actagggcaa tgctcaacca tagacgaagc taaagaatta ttggctacta
     4681 tcaatctagt caatatcgat tttagtgaaa aactaccctt gtcaccttta cactggttat
     4741 tagctgataa agaaaaatcg atagtaattg aaagtatgaa agatggactt catttatatg
     4801 ataatcctgt cggtgtgttg acaaataatc cgccatttga ttatcaatta tttaatctga
     4861 acaattatcg ctcgctatca aatggaacac cagaaaatca tttttcaaac cagatcagct
     4921 tgaatgttta tagtcgtggg atgggaggat tgggcttacc tggagatctc tcttctgttt
     4981 ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc atcaggtgat tcagaatctg
     5041 aaagtatcag tcaatttttt catatactag gctcagtcga gcaacaaaaa ggagtatgcg
     5101 atactggaga aagaaaatac gaatatacga tttattcttc ttgctgtaac gttgataaag
     5161 ggatttatta ttatcgtacg tatgaagaca gtcaaattac tgctgtaaat atgaataaag
     5221 aagacttgga tcgtcatgaa ttggtcagtt atccagtcat aaaagagcaa cagataaact
     5281 atgtcaatta attaatgaat agatccgttt ggtatcatat tttctgatac agtcataatt
     5341 agacacgatc tttaggtcgt gtcttttttt gctggttttt ttggaaatag catccactaa
     5401 tattttttta acacgaagag aataaggtag cctcaaaaaa gaaaacatgg taaattagat
     5461 attgggactt tattttctgt aaaaacagaa actttcatct tttgttttca cgtgacgaaa
     5521 ttgtaagaga gaaaatattc gtccgctatt tattctatga taagatgcat atgaatgaaa
     5581 taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg cggtattgta ggttttgtta
     5641 acgacaagga caacaaaaaa acaattatta atacaatgat ggatcgaatc gttcaccgtg
     5701 gacctaatag ttcaggagaa tatatcgata aacatgttgc tttaggattc agaagattaa
     5761 gtattattga cttagagggc ggtactcagc ctatttacaa tgaagatcgg acaaagatca
     5821 ttatttttaa tggcgaaatc tataattacc agcctttgag agaagaattg attgcagctg
     5881 gccacgtttt tcaaacacat gcagatacag aagtcctttt gcatggttac gaagaatggg
     5941 gaacagaatt attgcaaaag atccgcggaa tgtttgcttt tgctatttgg gataatgaaa
     6001 agaatgagtt attcggtgca agagaccact ttgggatcaa accatattat tatgcagaaa
     6061 tgaacggaac atttatgttt ggttcagaaa tcaaaagttt tctgcctcat cctgatttta
     6121 acaaagaatt gaacaaagaa gcgttgaaac cttatatgac tttccaatat tctccattaa
     6181 acggcgaaac attcttcaaa gatgtctatc gtttgcctga aggccattat tatacgtata
     6241 aagatggaaa aatagacatt cagcaatatt gggatgcaga ttttgaaaca aaagaaacgc
     6301 attcccgaca agaatggatc gaaaaaatcg acgaaaccgt tcaagcttct attgaagcac
     6361 atacggtcag tgatgtagaa gttggttctt tcttatccag cggggttgat tccagttatg
     6421 taacctctgt attgaaacca gatcattctt tctcaatcgg gtttgatgat aaaacctata
     6481 atgaagcgat tgaagccaga aaactaaccg aactattgga cttagataat acagcagcag
     6541 tgatcgacgg agacatgtcg ttcaaagcat tccctttgat ccagtatcac ttagatgaac
     6601 ctgattcaaa tccttcttgc gtacctttgt actttttagc aaatcttgct tcacaaagtg
     6661 tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt tgcgggatac caaacgtatg
     6721 gattccatac gaattcaaaa ttcatccgtg tgattgctca aggattgaaa aaattaccaa
     6781 aaggaacacg ctataattta ggacgtaaaa tcggaaaaat gaaaaatttc catggacgga
     6841 tccatttata tgaatcactt gctcctgcaa aagaatattt tatcggtcat gctcgagtgt
     6901 tcgaagaatc agaagcatca gaagtgttaa caccaaaata tcaaacagca ccatctgtgg
     6961 atgaaatcat gactgttcat tacgagaaaa cagaaggaat caaagatgaa gtcaataaga
     7021 tgcaatatgt cgatcttcat caatggatgc caaaagatat cttgttaaag gcagataaac
     7081 tttctatggc aagttctttg gaagtccgag tacctttatt ggatatcgaa gtcatgaaat
     7141 tggctcaaca aataccaagt aagtttttat tgaatcaaaa caacacaaaa gatattttca
     7201 gacaagctgc gaacaaacat cttccggaag aatggtcgaa tcgtgtgaaa ttaggattcc
     7261 ctgtgccaat caaagcttgg ttgaaagaag aacacggcta cgaacaagtc aaagcattat
     7321 tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa aatcatgaag ttgctagatg
     7381 accatcacga aggacgtaaa gaagagcaaa gaaaaatctg gacaatcttc agtttcttga
     7441 catggtataa agtctacttt gtagatgaaa cgattccgca agcagaagca atcgactatg
     7501 tgactgtttg atgaaaatca aatatcaata aaaaagaggt tttctcctac cgtttaacac
     7561 tgagtgataa gaaacaattt caaaaaacag ctcttcccgt tttatagata ttatgactta
     7621 tggttcggag ttggcagagg aacaccattg tttcgaacat aagggggttg tgacacgatt
     7681 ttcgtcacaa cccctttaca tatgttcaat aatcgatttc tttttctttt gtcccatctt
     7741 ttttataaat gaacaaagtt gattctttgt tttgtgctac ttgctttcct ttttcgattg
     7801 cttctttttt tgtatcaaaa tggttgcttg ccttttttgc ccctttagat ttcacgatcc
     7861 attgttcttc ctcatattct acgatcacat ctgaatcaag cagttttcca gcacgaggat
     7921 tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt ttcgaaagtt cgtttttctg
     7981 attcactagc atcttcatac catttttttg cttgatcaat ggcaattggg atcgcacggt
     8041 tatcagggta cccttcatct aataatgcat ttgcgatgtc gattgctttt tttcttgtca
     8101 atttatctaa gtttttcatt gatgcaggaa agtccttcat gttccatggc atattagtat
     8161 ccccctttat taaaaaacat aatctatata aacaagtgta agggtattta tacaaaaaga
     8221 aaaagatttt gtttgagaag taaaaccgtt gtatttatcg tcaagtagtt ttacttaaca
     8281 aaatcgtgaa aaacactagc aatctagaaa aaatcatggt aagataatca agtctgagag
     8341 tcatcttaga ggattcttgt aaaaatggga gggaaaaaca tgcgcgtaaa catcacttta
     8401 gagtgtactt cttgtaaaga acgtaactat ctaacaagca aaaacaaacg taacaatcct
     8461 gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca aagttacttt gcaccgtgaa
     8521 acaaaataat gtttttaaag ttctcaattc cttgctggtc aaagaattga gacttttttt
     8581 atctcaaaac atctaaaatc ataaggagcg gataatatgt ttatttccat gtgggcacaa
     8641 gataaaaatg gtctgatcgg caaaaatggt ttgttaccat ggcgattgcc aaatgatatg
     8701 cgtttctttc gcgaacacac aatggatcga attttagtga tgggaagaaa gacctatgaa
     8761 ggaatggggg atctttcact tccttatcgc catatcatcg tgttgacaac gcaaccaaat
     8821 tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg atgaactttt gtcgtatagt
     8881 caaacgatct cagaagatat ctatgtttcg ggtggtagtc ggatttttca agaacttctt
     8941 ccttacactg gtcggatctg gcgaacattg atcgatagca cgtttgaagg cgatacgtat
     9001 attgggaata ttgatttttc agacttcgcc cttatagagg agtatgaggg gataacggat
     9061 caagaaaatc tatatgccca tcgttttcaa aaatgggaaa gagtaaaaaa tagggttgct
     9121 tcaaaagaag aataaaatct atgcgtctag tttgttaata agaaaaaaag catctcacga
     9181 ctaatcgtga agatgctttt tttagttaaa atcaatttag tttttctttg atatcattga
     9241 ccatttcctg actggcaata ggtccgttat atagccaact agattcaggt ccgaattgca
     9301 gtacatgatt gtctgtcact gcttttagat tcttccagct tggttcattg aacatggcag
     9361 catctttgtc actgttcaca aggatgatgt agtcagcatc taattcagct agcttttcag
     9421 aggaaacagc agaccagtca gaagtggctt ttttggagac ctcttccact agatcaggaa
     9481 caccaaattc cagatcgtcg tatacgattc ttccacttga ccgatttttg ctgaccataa
     9541 aggctgagtt gttggtcacc cacaacactg cagctgtctt gttctcgatt ttatctgcta
     9601 gttcttctcg agttttcttg atggattttt gataatcgga aatgattttt tctgcctctt
     9661 tttcttttgc caaagctttg ccgacatctt ttagttgttc ttcccatgtg acatctgttc
     9721 cgttttttac aacataagtt ggcgcgattt tgctatattg ttcgtatttc ccgccttcga
     9781 cagtagcgga agaagaaatc aacagcaaat caggctcaaa gcttaacact ttctcataag
     9841 gaaggtcata agaaatcgtt gggacatcct ttaaatcatc ttgcagataa tcttgaatgg
     9901 aaccactgcc aacagtccac tgggcgatag gtttttcccc tagtgcaatc agatagtctt
     9961 ctaagtaact gccgatgatc cgtttgggcg cgtttggaat ctctacttta tgtccaagtg
    10021 tatctgtgac tgtatgagta ctattggatg aagtcgtcga attggcagtt gtagctgttt
    10081 ctttatctga agagggggag caagcgctca gtactccagc cagcagcaat atgctagata
    10141 atgtgaatac ttttgttttt aatttcattt ctttcctcct aacataattg agaatctttc
    10201 tcattggtta ttattcctga tatttcgaac gctgtcaacc gcttcatttg gctatagata
    10261 tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa ttgagaatga ttttcagcga
    10321 aaggatgggt gaaatgacga gattaagagc agctgacatt gcagccggct acgaaaacaa
    10381 aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg aaaatcacta gtttgattgg
    10441 gccaaatgga agtggaaaat ctactttatt gaaaagtttt acccgtatac tacctgtgaa
    10501 atcaggaaag atcattgtca ataactctga tatctctaca tacagttcaa aagaacttgc
    10561 aaaaaaaatt gcattattag cacaaactag tgaacaccct acaggtatga cagtagaaga
    10621 agtagtatct tacggacgat acccttatca aaaactgttc agtggcatgg acacgcaaga
    10681 tcatgaagca attgagtggg cgttggatgc tacaggtcta gctggatttc gtatgcggga
    10741 tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg atcgctatgg cgcttgctca
    10801 ggaaacagat atcttgatct tggatgaacc gacaacattt ttagatccgg cacaccaatt
    10861 agagatccta cagttattga ctgaaatcaa tcaaatgaaa caaacaacga tcttgatgtc
    10921 gattcatgac attaatcatg cttcacgttt ttcagattat ttattagcaa tgaaaaacgg
    10981 taagttgatc acagaaggta cacctgaaga agtgatcaca aaagagtgta tgaaaaaaat
    11041 atttgagatt gatgcaacga ttgtcacgct tccaagaaca aataaaccag tttttctaac
    11101 ctatgatttg ttgccaaaag agcaggagga tcaattatga ggactatgaa gaaaaataga
    11161 ctcctgcttt tggtctgttt tgttcttttg attatttttt ttgttgctct ttcaaatggc
    11221 gcagcgactg tgggaaaagc agatttttgg caggcgttgt ttgattttga tagatcaaaa
    11281 caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg tggcgggagc atttcttgta
    11341 gggagttgtt ttgcattgag cggtgcattg atgcaaggag tgacaagaaa tcctttagca
    11401 gattcaggct tactaggaat caacgcgggt gcttcgttgg caatggccct tagttttgct
    11461 ttttggccta aagcttctga tttttctgtg tttctaatgt cattggcagg ttcttttgct
    11521 gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa tgggcatgaa tcctttacag
    11581 ctgattttag ctggcgtagc tattagttct ttttttactg ctatcagcca agcacttacc
    11641 caattcttta atctgcagca ggatcttgct ttttggtttg taggcggagc tgccaatatc
    11701 acatggaaac aattagcagt attgtttcct ttatatatac ttgctgtttt ggtttctttt
    11761 tcattaggaa aagcaatatc tttgattcat ttgagtgaaa cacatgcatt agctcttgga
    11821 agtcatccta aacgaacgcg gttgattgtt ttctgtctag ttgcagtgtt agcagccatt
    11881 gccgtttcgt tagtcggacc tgtttcattt attgggctgt tggttccgcc agttgttcgc
    11941 ggaatggttg gaacggacta tcgttatgtt ctacctgtct cttttttagt tggcggtgga
    12001 ttggttatgt tggcggattt tacagcgagg atgatcaatc ccccttttga aacacctttt
    12061 ggattagtca ttgcattgat tggcgttccg tttttactga tacaagtaag gagggaacaa
    12121 cggtgaaaaa aatatattgg acgttctatt tcttgctttt tgtacttgtc atcctgtcta
    12181 taggtagttt aatgattggt acgcctttta tttacctgga tcagcttttt gccacgttca
    12241 tagggaaaga atcatcttct ttggcgctga ttgttagaga atttcgagtt cctcgattac
    12301 tgatcagttt attagcagga gcttgtcttg gcgtttctgg ttatatcttg caaggagtaa
    12361 caagaaatga attggcagat tctagtatat tgggcattaa tgccggagca ggatttttag
    12421 tgatgcttta tttaggtttc ttttcgcact attcgctgcc gtttctattg ctagttgtag
    12481 cctttctagg tggtgtttta gctgcttgct gtgtctatct tgcggcctat gatcgaaatg
    12541 gctttttggg tatgaatcga atcttattgt ccggtattgc cgttaatgcc ggcctttctg
    12601 cattaacatt gctatgcacg atccaattat ctaaggaaaa ttatggattt gtgaatgcat
    12661 ggttagcggg atcaatctgg ggtgcgagct ggccttatgt ttgggcattg cttccatggg
    12721 cggttatctt aattccgcta ggaggacttt atgcacagag gattgtattg ctatcatttg
    12781 gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa aagtcaaaaa atccttttgt
    12841 tgttagctgt tgctttggca agcggtagtg tagcggtgac aggtagttta tcttttgtag
    12901 gactactagc tcctcatgct gcgaagctag tcgtacgtaa agaaaataac tggacctttg
    12961 ttctgagcgg tttgttcggt tccatttttc tgcttagtgc agatatggtt gctagagtga
    13021 ttctactaag tggggaagta cctacaggaa ttttgattgc cctttttggt gcgccatatt
    13081 ttctgtatgc actttttatc agacaaaaac acgcgctgaa cagttgaaat tcattaaaaa
    13141 aggaaggcca atcatttcta ggtcttcttt ttattctatt tatgcattta tttgaaagtt
    13201 cgattgctat ttttcttttt ttcctatacc attttatgag agaaggaagg gtgagttttt
    13261 ggaccaattg aaagtaagat tacgccggaa aggaatcgaa aagctgaaaa atctggccaa
    13321 aagtggcaag aaaaaggaaa aagaacagcg aatctataaa cagctattcg ccagcgagta
    13381 ctggaagtca gcaaaagtga tagggacgac tttatcgaat gaatttgaac tggatacaca
    13441 gccggttatc caacgagcag aaaaagaaca aaagaaaata gttgtaccta aaactttgcc
    13501 gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc gttcttgtac gttcgagttt
    13561 tggtgttctt gagccacaat cagagaagct ctatttgccg gagatgatcg atctcctcat
    13621 tgtaccaggt attatctaca atagagccgg ttatcgaatc ggttttggcg gtggttatta
    13681 cgaccggtat ttaaataatt ttcataaaaa aacatgtagc cttgtttttc gtgaacaatt
    13741 gaatgaacaa tggcaaccgg agagctttga tcagaagatc caacaattgt ttattgacta
    13801 aaatgaaatg ggaggttttc atgaattatc aacagcaaat aaaattgcgt gcatggttaa
    13861 gacgaccgtt atggacctat gcttttttag ggatacagac agcggtcttc ctcatgatgg
    13921 aattatttcc gcgacttgag atcccttatt atgccggaat gtatggtcct tatcttgttc
    13981 acttcaatga atggtggcgt ttagtgacac caatcttcat ccattttgga ctgatgcatt
    14041 tcgtaatgaa ctcactgata ctgtatttta tggggcagca aatcgaggca atctatggcc
    14101 attggcgttt tttcttgatt tatatgttca gcgggataat gggaaataca gcaagctttg
    14161 cttttaacga agcgaatgtt ctgtctggtg gtgcaagtac ttcgatattc ggtttgttcg
    14221 gtgcgttgtt tatcttaggg tttcatttta aacataaccc agcgatccag cagctagtca
    14281 gacattttct tttatttatt gtgttgacat ttgtttttgg tcttctggat acctctgtag
    14341 atgtttgggg acatgtcggt gggctaattg gcggattagt gttagggaat gttttgggtc
    14401 taccgaaaca acagacaagc tattcgatac accaaaggat tctttctaca ctagtttttg
    14461 tatttctttt tgtaatatgt attttactgg gtttaaaaaa atatggttta cttgtataat
    14521 atgaaggaag tgcttaaaga tggagagctt atatgatgta cagcagctgt tcaaaaaatt
    14581 tggcatcttt atctatgtgg gtgcacgcat ctatgacatt gaattaatga ctatcgaatt
    14641 gcgtaatctg tatgatagcc gtttgattga tcatgataca tacatgacag cgtggcgcat
    14701 attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa ggaaatttgt aatggacaag
    14761 aagattattg ggattgattt aggtggaaca acagcaaaat ttgcaatttt aacaccagaa
    14821 ggagagattc agcaaaaatg gagcatcgat accaatattt tggatgatgg taagcatatc
    14881 gttcctgaaa tcattgaatc aatcaatcat cgtttgaatt tatataatat gaaagccgaa
    14941 gactttatcg gaatcggcat gggtacccca ggaagcgtgg acagcgaagc tggaacggtt
    15001 attggtgcgt ataacttaaa ctggacagaa gttcaatttg tgaaaaaact gatcgaggca
    15061 ggaacaggaa tcaaattcgc tatcgataat gacgcgaatg ttgctgcttt aggtgaacga
    15121 tggaaaggtg ctggagaaaa cgatccagac gttgtattcg ttactctagg aacaggtgtt
    15181 ggcggaggta tcgttgctgg cggaaatttg attcacggcg tgagcggagc tggaggagaa
    15241 atcggacata tcactgtgga tcctgaaggg ttcgaatgta cttgcggtaa acgcggatgt
    15301 ttagaaactg tatctagcgc tacaggtgtg gtacgcctag gtcgtttctt ggcagaagaa
    15361 tacgcagggg attcaaaact gaaagcaatg ctagacaacg gagaagaagt aacaagtaaa
    15421 gatatctttg aaatggctca agaagacgat ccgtttgcat tgatggttgt tgatcgcgta
    15481 tgcttctact taggtttagc ttgcggaaac ttggggaata cattaaatcc gtcaagtatc
    15541 gtgttaggtg gaggagtatc ggcagcagga gaatttttac gtagccgtgt agagaaatat
    15601 tttaaagaat ttactttccc gcaagtaaga gaatcaacaa aaatcaaatt agcagagctt
    15661 ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct tgaaatttgc ttaaaaaaac
    15721 ggaggcatta catggtttta tgggtaatta atatcatttt gctattgatt gtgcttgcaa
    15781 tcatcctttg ggaagtctat ctacgagtaa tggccaaacg ttcagcaacg actttgacag
    15841 aagaagaatt tcgagaagga atgagaaaag cacaagtaat cgatgttcgc gaaaaagatg
    15901 tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc ttacactgtt ttgaaagatt
    15961 cattaggatc tattcgaaaa gaccagcctg tctatattta cgatcaaaag aaaagtttga
    16021 gcatccgtac agcaaataaa ttacgtaaag caggttatca agagatttat ttacttaagg
    16081 gtggatatga cggatggtct ggaaaaatca aaagtaaaaa agtataaaac agatgattgt
    16141 atgacagctt taaaaaccga atcaatcatt agattttgac caagatcaca tgaacggcgc
    16201 aatggatagt tgtgccgttc atttttttta ttttgtgcta ttatctacat atataagcta
    16261 agcgaaaagg tagaaagaag gaaacaacta tgatcaaatt aaaacgtgtt taccttgatg
    16321 ctgaaaaaga ggatggctat agaatactcg tggatagatt ctggccacga ggaatttcaa
    16381 aagaaaaagc gcagttagat ctgtggttga aagaaattgc acctagtaat gaagcacgca
    16441 aaacttttga ccatgtacca gaacgatttg cttcatttgc aaagaaatac caacaagaat
    16501 tgattttcga agaagaaaag aagcaagcta tttttaaact cagtcaactt atgaaagaac
    16561 atccagtcat tacttttgtt tatggtgcaa aaaatgaaca tcagaaccat gctgttttat
    16621 tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata gggatcattg gtgccggacc
    16681 tagaggatta agtgtagcag aaaggattct ccataataat caggacagac attctcttga
    16741 actttttctt ttcgatccca atggtcctgg aggtcgagtt tggcggttga atcagccacc
    16801 ggagttatta atgaattctg tttctcaaca agtgacgctt tttacagatg aaacattgtc
    16861 cagtggagga ttggtaacac agggacccaa cttgtttcaa tggagtaaag gacctgctac
    16921 agattatatc gaacagacgg taaccaaaaa taagcaacta attatagaag aaatcaatcg
    16981 tctggaaaaa gatcagcaat ctactcgatg tttatacgga ctctatcaac gctggttttt
    17041 ctcaaagttg caagaagaat ttccagcttc tattcagttt gtttattctt tagtaaaaaa
    17101 agtaagcaaa agaaaagatg gttatctttt acagacagat cagcaaagct attctatcga
    17161 tcagcttgtt ttagcaagcg gtcactggga aaatgatgag acagaagaag aaagagtgtg
    17221 ggtagactac gcaaagaaaa atgttttact gtatcagctt ccttctaatc ctgcagatgt
    17281 agatctttcg aagataccgc ctaaagaaaa tgtcttttta agaggtctag gcttaagttt
    17341 ttttgattat gtcggattgt ttacagtatc aagaggaggg cgcttcgaga acgaaaaggg
    17401 aaaactggtc tatcatccat caggcaagga accgattgta tacagtggat caagaagggg
    17461 gctcccttat taccctcgag gaagaaatca aaagcaaggc ggagcaatgg catggccacg
    17521 gttactaaca aaagaaaacc tggagcaatg gcatcagtcg ggatttttga ctggcgaaac
    17581 attttttgac tatctgaaaa aagacgttga gttgttttat tacaagaaaa tcatcgaaga
    17641 atatcacttg ccgattccaa agaaaaattt tgagcatgat tttctctccc tctcttcaga
    17701 ggagtgtctc ggaaagcatc cagcactaat cccttataga tgggattggt cgtatttaga
    17761 aactcctctt gcctatcaga atgagccctt ttcagaagct agccgaatat ttattgaagg
    17821 gcagataaaa gaagcggaaa aaggcaattg cacaggagca tttgcttcaa catttgatgc
    17881 attaaaagac tggcgtgatc caatccggca ggctattgaa tggcacgttt tttctgctaa
    17941 ggaatacaaa gaaatactat ggggatggtt tactcatcta aatagctttt taacgatcgg
    18001 tccgccaatc attcggacga aagaattagc agcactgata gatgcgggta tttttcattt
    18061 gatcgaaccg ccatttgata ttcagcaaaa gcacaaccgc tatgttattt ctctacaaga
    18121 taaagaaatc agcagtcgct atttgattga agctcgattg ccacaaacaa gcttttctcg
    18181 ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa cttgttcgcc ctttttcatt
    18241 ttcagataat acaggaagtt atcattcaga agctatagat gttgagcgag gtacgaatcg
    18301 cgtgattgat gcttcagggg aaatccagtc tgacttttat tgctatggaa tacctgttga
    18361 aggtgtggat tggctaactg cagcagtagc tagaccttat acagatccat ggaatctgag
    18421 acaggcagat aaaatagcac aacttatatt gactgacaaa taaaaatacg catgatgact
    18481 tatctgtttt ttagaaagtc ttcatgcgta tttttaaata attattaacg agaaacgcgt
    18541 ttacgcattg cttttttacg gttttcttcg atcattgctt ctttttcgtc aattggatcg
    18601 atcactgttt ttttcacaga agcaaccaaa ccagcaacgg cagcggctgt tgccagactt
    18661 cctactaata ttccagatac aaattttttc attgaatcca cctcatttat tatttgttat
    18721 atctattatg gaacaaaaga gaaaaaaagg ctagttttac gtttgtttgt aaacctttgc
    18781 aaaaaatcct tg
//
LOCUS       DACEKQ010000058        18729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEKQ010000058 DACEKQ010000000
VERSION     DACEKQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.062"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            283..522
                     /locus_tag="IW671_001809"
     CDS             283..522
                     /locus_tag="IW671_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898792.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IW671_001810"
     CDS             complement(884..1144)
                     /locus_tag="IW671_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898793.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IW671_001811"
     CDS             complement(1329..1826)
                     /locus_tag="IW671_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0898794.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW671_001812"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW671_001812"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0898795.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW671_001813"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW671_001813"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0898796.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="IW671_001814"
     CDS             complement(4548..5210)
                     /locus_tag="IW671_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0898797.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="IW671_001815"
     CDS             complement(5220..6035)
                     /locus_tag="IW671_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0898798.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="IW671_001816"
     CDS             complement(6297..6740)
                     /locus_tag="IW671_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0898799.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="IW671_001817"
     CDS             complement(6874..7212)
                     /locus_tag="IW671_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0898800.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="IW671_001818"
     CDS             complement(7200..7577)
                     /locus_tag="IW671_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0898801.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="IW671_001819"
     CDS             7801..8994
                     /locus_tag="IW671_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0898802.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="IW671_001820"
     CDS             complement(9160..9582)
                     /locus_tag="IW671_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0898803.1"
                     /translation="MQTFLTKASQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10172..10627
                     /locus_tag="IW671_001821"
     CDS             10172..10627
                     /locus_tag="IW671_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898804.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(10789..11961)
                     /locus_tag="IW671_001822"
     CDS             complement(10789..11961)
                     /locus_tag="IW671_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0898805.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12167..13596)
                     /locus_tag="IW671_001823"
                     /pseudo
     CDS             complement(12167..13596)
                     /locus_tag="IW671_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13593..15095)
                     /locus_tag="IW671_001824"
                     /pseudo
     CDS             complement(13593..15095)
                     /locus_tag="IW671_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(15092..17419)
                     /locus_tag="IW671_001825"
     CDS             complement(15092..17419)
                     /locus_tag="IW671_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0898806.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18262..18555)
                     /locus_tag="IW671_001826"
     CDS             complement(18262..18555)
                     /locus_tag="IW671_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0898807.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5432 a         3669 c         3217 g         6411 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacattc ttgcatttca gcaatttttt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa gttattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggctac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaag cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca aaacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagctcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag aagagaaaca
    10141 aaaagcggaa aaagctgcac tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca
    10201 agctgaagca aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct
    10261 acctgaaaac aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc
    10321 aaaagaagca gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca
    10381 agcgcaacaa gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aagcagcagc
    10441 tgaacaggcc tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt
    10501 ctatgtcaca ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac
    10561 ccctgccact ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg
    10621 aaactaattc aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa
    10681 gctggcgttc cagatagtaa accaaaaata gcagtttaca cgtccatttc gtcagcttct
    10741 ctcttttttc atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc
    10801 aatcaaatag tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat
    10861 tgaaaactgt ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg
    10921 caagcgatag ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc
    10981 tttcacaaat ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga
    11041 aaagaccatt ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata
    11101 gtttcgctta gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat
    11161 tgatccaagt agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat
    11221 tccttggatc gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag
    11281 cagtcgatgt gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc
    11341 tttttggata cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg
    11401 atacgccaat gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg
    11461 caaatctgta aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt
    11521 gtttttcccg ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa
    11581 tatttccgtt gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac
    11641 tccaagcata tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc
    11701 aactgcttgg ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt
    11761 tttctttgtc attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg
    11821 cttttgataa tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa
    11881 aggataagaa aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat
    11941 tcttattctg cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg
    12001 tgaaatcagc gccttgacct gagtattcgc tcaacaaacg gcaaaatagt tcctcctatt
    12061 ttgccgtttg ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt
    12121 tgaatcacta tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt
    12181 cttgatggcg ttttttgaag tagatcactg ctcctccaac taaagcagta cctaccaaga
    12241 tgaatacgat gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga
    12301 tcgttgtttc atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt
    12361 cattcttagg taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tatgcccctt
    12421 gtttcagccc ttcgattgaa aatttccctt ctgaatcaga aatcaatttc acttcttccc
    12481 agtcagaacc tgtgacttca tctgaccaag catatgggta ttctcctgag gcaagatttg
    12541 ctattttttt cattaattta acagttggat cttttgctct tagaatagaa aattctgcat
    12601 cggctaattt ttcttgcgtg ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt
    12661 ggacattttc tacagtaatc gtgttatccg tagttgtatt ttcccgattg actacaccgt
    12721 ttggattccc aagattggct tcatctgcgc catggatgat accggttgtt tgttgcaatt
    12781 ttccgttttt tactataaaa gtagtttctt tttgtggatt ggttaattga taaccatctg
    12841 gtgcttttgt ttcaactaaa gtataattgc cgtctattaa tccattgaca ctgatggatc
    12901 cttcagtatc agttgtgaat gtcaacgctg actcagcagg atcatagaga gcgacaccag
    12961 tgttttcatc aaaaccggtc acgaattgat tctctgaatt tttgatctgg aattgcgccc
    13021 ctgctaactt atctacccct gaaaagttcc cagctttctg gattttgata ttgctttctt
    13081 tgatgacatt tttagcatat aggtgaatcg tgtctaaagg tttgtcaggg tctgatgaat
    13141 tcgggttata aaagggtaag cttaacacga tatttggcga cgtttcgaca ccgggctttg
    13201 gtgtttctac aaagacgtat acagcatctt tcccatcaga tttacctgga agagtcagga
    13261 tagctaatcc ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct gctggaactg
    13321 ctgtgtcttt acttggatag tacgtagcag tgatgtcata tacgttgaat tcaacgccgt
    13381 tcaacggttc gctattcgga aatgtcaatt ccgttccgtt gtttatttga ttttctggtg
    13441 ctgaagtgaa tttcaattta tgcaaattaa ccgtaacatt ttctggtgta ggtgatacgg
    13501 cctagaaacc tctgcagttc ctaggccgag aacgaaagga agcagcatga tagcagttcc
    13561 taaaaacgtc catatttttg ttttgatgtt tttcatttta tttccctcct attgtttcct
    13621 ttttgttgtt ttttataaaa ccaagctgtc cctgttgaaa ggactagcaa caaccctaaa
    13681 attgagaaac ctccaagcat ctcttcgctt gtttttggaa agcttcctcc atttttttca
    13741 tttgatggac catttgtctg catctgaact ggcgtttttc cattattcgt cggatttgct
    13801 tttggttcgt tgatgatttc caatggtata ggatgaagcg gatcataact gcctttttca
    13861 acttcaaaga gaatggttga ttgactcaat atatatcctt taggcggttt aatctccact
    13921 agttcgtaac ttccaaaaga caatccatgg acagaaaatg ttccatcttt actggaagta
    13981 aaaatcgttg ctcttgaatc atctttggct acccaatgat ttatcccgtt ttcttgcgct
    14041 aagtattggt catttttttt gactagaaat tgagcatctg ctagtttttg tttcgtttgc
    14101 cgatccactt tgacaaattg tttgcctcct gtttggatgg caagatgtgt ttctactgtt
    14161 tgattgtcag tcgtcaaacg tacatcgtta tcaaatgtcg ttttttcact gttttctttc
    14221 aatgtcattt ggtaacggat cgttaatttt ttcccagcaa acgaactaag atcttttcca
    14281 gaatctgata agacaaaacc atgttttgtt ttttgtgtgt gaaatgtatg aatcttgtct
    14341 ccaccaatca aaaaatcgat cgtttctggt tctagccata actgaggatc agctgtatct
    14401 ttgatcgtat aggttcctat ctcgttgatg ttctcaggaa ttgttacggt gatgagataa
    14461 ggaactttat ccccatctgc aaaatctgtc tgcttattct ctactgtttt tgttaatgtg
    14521 agtgcagcag gtattttttg ttcgctcttt ggatacaagt ggatggtcgt caatttatga
    14581 ttagatgaat ctgtcacagg aagcacaacg acaaatggtg ctgatttttc ttttacttaa
    14641 tctgaagtct ttatttccgc aaatcggtaa acagcgtcac gaccttgctc atctttgtcc
    14701 gatgcactaa aagaagcaat gccctcttcc tcgtctactg tttttgtcac attttctgcc
    14761 agtatttttt cactatcact tttttgtgct agttttcttt gtgcgtcttc cacactactt
    14821 ccttctgacc gcagcttgta aaattcatcc gagacatcat atatagcgaa agtaacgccg
    14881 ttcaaccctt ttactccttg aaagttatcc gggatattta ttccgtcgtt ttgactgata
    14941 cttggcatct tatcgctttc gaacattcga ttatgcaaag taaatgtaat aggtgattct
    15001 ctagctgaca cagtcggaga acctataaaa aagaccagta ggataaaaaa gagaacagca
    15061 aatatatcca cacgtacatt ttttgttttt ttcatcgctg ttcacctgct tttttcttac
    15121 gatggataaa ataaaagaag ccagaaaaaa taaagatcag tcctaaaaag acaaagggca
    15181 gtagacctat cccacctgtc gaaggaagca agagagtcgg attgatcgtc aacttagcgg
    15241 tatctgttgc gataatcgta taattattgt ttacacttcc ttcgcctaaa ctaatcgtta
    15301 ctatttgtga tccaatatct gtccggctgt taactgttag ggtataatcc aatgattcgc
    15361 ctaagacaac accggtaacg tggagatttc caaccgaaat cgagtgactt tgggcatcat
    15421 aattaaaggc agcgtcatcg acagtaatca ctgcgttagc tttatagatg gtataagttc
    15481 ctgatgtgtt ggctatatca gtaaatgaca acttgggatt tgctgctttg actttgtcta
    15541 ttccgctatt cgttagttta attgtatagc tgccagcatt aaccatattt tttgctgatt
    15601 gtgcatctgc tgtgtaatcc atactgctaa gtgccacttg tactggagta agcgaactag
    15661 tgacaccctc tgctagcttg acatggattg acgggacacc tgcttttcca tcatatgtct
    15721 tactgccact gagctctacc gcaccgctga tttgcttaat cgtatagaca tcagatgtct
    15781 gcacgatagt aaataaatag tttgttctgg cagctagttt agctagtccg ctttttgtca
    15841 attgccaaga cgtataggtt ccaatttcag taggcgcagt tacttcaact cccttttcat
    15901 tcatcgcttt gaaatcagtt ggattccatg tgatttgatc aatactgaga tcattgttta
    15961 aaagggatgt tgattctgtt ttatcattaa aggcatattt aacgatcggt acaacagaaa
    16021 gaccatcata gtacttactg ccatttgcat tgattgatac tgttgcattt gtcttataat
    16081 ataggatgac ttcagaaatc tgttttccat ttgcgtcctt tacgattaca tttccatttg
    16141 tatctaaact agctaaaaca ctcattgctg attggttctt tgtttctgtc gctgaaacag
    16201 ctgtaaaggt atatccgttg atagtaggcg tattagtcgt caggtcttgt gcagcacttc
    16261 cgtaagtaag attcattatt ttagaaggag caatcatctg tccaaaatag tcttgatact
    16321 taatagtgat tggtgtttgt ttgcctgtat aggtcaccgt atcgacgtta ctttctaggg
    16381 ctgtgaaaga accgtttgtt tgtgcaaaag aatagcccgt tactgctgtt ggagtgataa
    16441 ccactttgtc acccacatgg attccagata atgtagtccc gtacgttccg atttttgctg
    16501 ctccaccatt gactgtataa gaaaaagttc ctgtccgatt cgcattgacc tgaaccgttg
    16561 ctgcttgata ggttggaaaa tttgaaacat ttcccttacc atcaacgatc tgcccatttt
    16621 tgatcgagta ccccgtgaat ggaatatcat ttgctaactg tataccgatt gaaaaagtac
    16681 tcatacctcc tccggcagga agccctcccc atgttgaggg tgtaaaagaa taacttttat
    16741 ccgcattggg tgtaactgaa tcgatcgtat ttccagcggt agccaccccc atatagatac
    16801 tttttgggaa tgtgatagta gtaccattta ctgtggagcc aataaacaga ttatggatat
    16861 ctggacggtt atcaataaaa gcgattgctt tggttacgcc ttgtaacgca gttttatccg
    16921 cactcatcga tccgttaacc gcaacaatga ctccatcttt ggcatatatc actgtatatc
    16981 catctggtat ggtatgtccg ttgaaattcg cagtggccgc aacagtacta aatgtctggc
    17041 ttctacctgt ttctgaataa gctttctccc ctgctgcttt tgctgctgtg atttcttttt
    17101 ctgtcggatc aatcagttga gtcgaggatg tggttgaggt tgtctcgttg ttgctagact
    17161 gattggtgct ttctatggac tgtatttcag aatagggtgg actatttgtc tcctctgtcg
    17221 tttggttagc aaaatttgac gtttctgtat aaacgtttac ataaatactt gataagctcc
    17281 ctaagaaagt aaaaatcaag atgatcgaaa tcataatggg atgtaagtat tttcttttag
    17341 atacagcttt gagtctagaa acaaaatgtt tttgacatat acgttggatt ttattttgag
    17401 ttttcttaat cattttcatc cctcctctca ataaaaaatc atcaaataat cctttacacg
    17461 ttattctaaa tttaattttt attattttat tgatgtatag aaagtttggg gctttttttc
    17521 tctttttatt ctaaggttat tttagtcttg tcctttaata caaagatttt atttttaaat
    17581 aaaaaatact ctttttttac gattattgaa aaaaaaagat aaaacttgat taattgaagt
    17641 attgcttaaa tttggaagct atgagggaag aataaaagag ttaggacggg attatctact
    17701 atttttcttt gttttaaata tttgttatct ttgaacataa aagagaaatg tgtcgactgc
    17761 agtgttgtgt ccctcatcac gaataaacgg tgttcaatta gctgatcttg tattcactta
    17821 aaaaatggta aagtacgagt tgctattccc cattttcaat tttctaacgc tcaggacaaa
    17881 atactgtttt cacaacctca ttggaataaa agcatatata ttgtttcaag aacgaaatct
    17941 tgttatcttt ttaaagggca tctgtccaaa atctaatttt aggaggtacg ttttggtatt
    18001 tattcgtaca aataaaagag aattatcttt attcatgtaa cgcatccaat tgattttttc
    18061 atgtacgctc ctggaacgat tttactgacg attgcttatt taactgcgaa tcgctcatta
    18121 gcttttatca tagctatcca tattttgaat aacgttcttg gctttgtatt atagatacag
    18181 atgacaaaaa aagttcaagt atgcggtgta gaaccaatat cgcatacttg aacttttttc
    18241 aatgagtata aattaaaact tctaattttc aattcccatc atctgatatg cccgaccgtc
    18301 tgaacttctt ttgaaaaagc caaatgtgat tagataacgt ctttgttcga cataatcgcc
    18361 actagaaaat acgttcagca gtatcgaatt aacctctgtt tctgtataag tcactccatg
    18421 gtcaaagcgt ttcaagactt ctttcaatag gatgtatttc ttttttgttg aacttggtac
    18481 ttgttttact tttccatcat tcatgaatgt gtctaaaact ttcatctttt cttgctccgt
    18541 ataagtataa accatcttct ttctcctttt gattctaaga atactccatt tatgttagaa
    18601 tattctggat tttttattaa agcggttact cttaagaaag atattatcat agtgctcttt
    18661 tttttactac tacttatagg tagatttatt ctaatccatt attctcccct accaataatt
    18721 aaagaaaaa
//
LOCUS       DACEKQ010000059        18347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEKQ010000059 DACEKQ010000000
VERSION     DACEKQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.063"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(139..705)
                     /locus_tag="IW671_001827"
     CDS             complement(139..705)
                     /locus_tag="IW671_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0898808.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(705..1661)
                     /locus_tag="IW671_001828"
     CDS             complement(705..1661)
                     /locus_tag="IW671_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0898809.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1661..2317)
                     /locus_tag="IW671_001829"
     CDS             complement(1661..2317)
                     /locus_tag="IW671_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0898810.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2687..4705
                     /locus_tag="IW671_001830"
     CDS             2687..4705
                     /locus_tag="IW671_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0898811.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5132..7546
                     /locus_tag="IW671_001831"
     CDS             5132..7546
                     /locus_tag="IW671_001831"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0898812.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7894..9447
                     /locus_tag="IW671_001832"
     CDS             7894..9447
                     /locus_tag="IW671_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0898813.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9828..11612)
                     /locus_tag="IW671_001833"
     CDS             complement(9828..11612)
                     /locus_tag="IW671_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898814.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11627..13348)
                     /locus_tag="IW671_001834"
     CDS             complement(11627..13348)
                     /locus_tag="IW671_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898815.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13448..14209
                     /locus_tag="IW671_001835"
     CDS             13448..14209
                     /locus_tag="IW671_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0898816.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15176..15444
                     /locus_tag="IW671_001836"
                     /pseudo
     CDS             15176..15444
                     /locus_tag="IW671_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16116..>18347
                     /locus_tag="IW671_001837"
     CDS             16116..>18347
                     /locus_tag="IW671_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898817.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6017 a         3272 c         3713 g         5345 t
ORIGIN      
        1 tttttgattt ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga
       61 ggttatgaaa tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt
      121 atgttttgcg ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt
      181 cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca
      241 aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag
      301 gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt
      361 ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc
      421 tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa
      481 caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc
      541 ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa
      601 aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat
      661 ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt
      721 ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca
      781 ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca
      841 tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg
      901 acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac
      961 attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct
     1021 aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg
     1081 atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa
     1141 gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat
     1201 ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca
     1261 tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact
     1321 acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg
     1381 aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc
     1441 tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca
     1501 ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct
     1561 ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa
     1621 gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt
     1681 aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag
     1741 aaagactgag taagtagccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc
     1801 ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg
     1861 ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc
     1921 ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag
     1981 gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa
     2041 atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg
     2101 cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt
     2161 agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag
     2221 aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga
     2281 agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg
     2341 acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg
     2401 attcctcttt gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt
     2461 tttggtaatg caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa
     2521 acaagaacgt agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat
     2581 tacaatatga ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga
     2641 agttataatt attaataata gataaattga agttggagga tagaagatgg aaaacattgc
     2701 aagaaaagaa cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc
     2761 agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt
     2821 tcctgttcaa gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt
     2881 tatcacagaa attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc
     2941 agtgatgatc gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc
     3001 accttattat aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga
     3061 tacgcttgaa tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc
     3121 ttcttttgct gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa
     3181 cggttattac tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc
     3241 ttgtttgaca gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat
     3301 cgcaacttac ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc
     3361 gactacaggt ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg
     3421 taatacaaac tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt
     3481 ttggggaatc tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat
     3541 ccaaaacaat ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg
     3601 ctcttcgacg aataattccg gtaacaatac caactcttca ggaaatgctg gtacatcaaa
     3661 cggaggacaa acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat
     3721 ctcaaatgat cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa
     3781 tttcgtttat ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc
     3841 aaatactggc tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc
     3901 taatacagct tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc
     3961 aaataaatac ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt
     4021 tatctaccca ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg
     4081 taatacaggt tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc
     4141 gtctcaaggc acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa
     4201 cggcatcacg atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc
     4261 aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac
     4321 ttctgcagcg aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg
     4381 taatacgatg tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca
     4441 tatcacgatg gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg
     4501 acagcaagtg attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg
     4561 ttcgaaaacc tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg
     4621 aattacaatg gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg
     4681 tcaaacgttg attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac
     4741 aaaaatattt gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa
     4801 aaagactgaa gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact
     4861 cgcctttgag ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca
     4921 acgttcgagg ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata
     4981 aaaagagcaa tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag
     5041 caaaaggaaa taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac
     5101 aagtgttttc aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa
     5161 aaaatggcaa aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa
     5221 gccaaaattt tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg
     5281 gcatcctgaa ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata
     5341 caacgtgctg catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct
     5401 agatacagga aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca
     5461 aatcaattct ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga
     5521 gtattataaa tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga
     5581 agcagaagta gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt
     5641 gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg
     5701 gatgctgaaa atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg
     5761 gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat
     5821 tgaatttaaa gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac
     5881 tctattcggt gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac
     5941 gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga
     6001 tctaaagaga acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat
     6061 caatccagtt aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata
     6121 cggcacagga gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca
     6181 aactttcgga ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta
     6241 cacagaagat ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc
     6301 aatcgataaa atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta
     6361 tcgcttacgt gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat
     6421 ccattgggag gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc
     6481 taagacaaaa aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga
     6541 gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat
     6601 gccacaatgg gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa
     6661 agaactagca gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg
     6721 agcagaacat gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga
     6781 tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt
     6841 aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt
     6901 ggtcgaaact tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga
     6961 tgcttcgatt gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt
     7021 atggcgtttg atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga
     7081 tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa
     7141 tcttcatttc aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt
     7201 ggacgctctg ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc
     7261 tcacatgggt gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc
     7321 atggccaaca tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt
     7381 aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga
     7441 aaaagcagca ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa
     7501 agttattgcg gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca
     7561 ataaggactg atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc
     7621 agattttttg ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt
     7681 tctcaaacac gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca
     7741 tcaaattttt gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag
     7801 tacataccaa attcataaat tttttattag tgggactagt gattgtatgg attttgacgc
     7861 ataatgaatg aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa
     7921 gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg
     7981 ttcaagcttt taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc
     8041 ctatatcgcc atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat
     8101 ttgtatcact tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt
     8161 ctacttattt ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa
     8221 agagcacaac agtttttaca taataaacca acttctcaag atgatgacta tctttatttt
     8281 gagaagactt cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg
     8341 agatcacttg atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac
     8401 acattccata tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct
     8461 ggtggatacc tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta
     8521 gctggaacaa tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct
     8581 gcttctcaca tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg
     8641 ttgttagctg atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa
     8701 cttgatacag agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt
     8761 gtgccgattg attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt
     8821 cctgaaggtt attggatgga caacacatat gatatttccg ccgatttctc atggtttatc
     8881 tattcttctg gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa
     8941 acgtctatta tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc
     9001 aacgatggtc tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt
     9061 tacactacta tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag
     9121 ccagtacaaa atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt
     9181 ttgttttata caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa
     9241 aactatcaga caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc
     9301 gcgaatacgt tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa
     9361 atgtttgcct gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa
     9421 ctatcgcaag tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc
     9481 gagtcacaag aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt
     9541 gattcggagt tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac
     9601 aagatcgtgt cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga
     9661 ttgtgacaag tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta
     9721 tcattagtat tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg
     9781 atcatggact aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga
     9841 aattgactat tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt
     9901 cctttttcga cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt
     9961 gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc
    10021 tgaatcaatg cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca
    10081 ggaggattcg ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac
    10141 gtgccgtttt cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga
    10201 tcacattgtg cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg
    10261 taggcgatat tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca
    10321 aaagaacgac gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc
    10381 tttcctttat tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca
    10441 ccagttggac caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc
    10501 atcaatattt tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc
    10561 ggttgttcta aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc
    10621 tcaaatatcc tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg
    10681 gtagaaattg gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc
    10741 gtcacaccac ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt
    10801 tgattaatga aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa
    10861 gcgttgtatt gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg
    10921 aatgttttga ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc
    10981 agttcatact gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca
    11041 attgaactgc caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac
    11101 agcatcagac cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc
    11161 aataacactt gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact
    11221 tgatgattgt caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc
    11281 accctcaatg tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg
    11341 atgctgctta agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg
    11401 atatcactga aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg
    11461 atcccgattc ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat
    11521 agaaacaatc cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa
    11581 gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg
    11641 attttgcaaa ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc
    11701 caacgatttt tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc
    11761 gttgagcaac aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg
    11821 ctgcatctgt tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg
    11881 gtttgatcaa cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac
    11941 cgccttgagc aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt
    12001 gagcgatctt gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca
    12061 agttgcttaa aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg
    12121 agcggatctg ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta
    12181 tcgtagggtc actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc
    12241 cgccaacgat cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg
    12301 gatcagagat ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat
    12361 caaaggttgg ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca
    12421 gtatttcttc tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag
    12481 ctgcgatcat caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac
    12541 cgatttgtaa tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa
    12601 cgattcccat gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga
    12661 tcatttgatt agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat
    12721 tatcaaaggc acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc
    12781 gatccatttt tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa
    12841 caatccatac ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac
    12901 gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa
    12961 ataaaacaat catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc
    13021 cagaactaaa acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct
    13081 cccgcgtttg gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg
    13141 cagcgattgt tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg
    13201 aggcttcgcc acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta
    13261 atgtaccggc gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt
    13321 tctgtaagaa tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga
    13381 ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg
    13441 agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa
    13501 atcaacgatc cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa
    13561 tgattatcga ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag
    13621 aaaattagat ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt
    13681 atatgagaca cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca
    13741 aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga
    13801 ggaagaattt cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga
    13861 tattgtggaa atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc
    13921 agcttccagt tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt
    13981 tactcaagac gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa
    14041 atccgagatg aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga
    14101 aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa
    14161 caatatcaga actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt
    14221 ttaagataac tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt
    14281 acgggtttaa tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga
    14341 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
    14401 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
    14461 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
    14521 aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta cattattaca
    14581 atatgttaat aaggcaaaaa taaatagatg gattgaataa acatattttt taatcaaata
    14641 gtccctactg tgactttcct cagctatcgg tgatactacc gatagcattt ttttgcttat
    14701 aaaataatta taagtattta gagatttttc ttctaacatt cttcaaacaa gaaaaatcac
    14761 aaaggttatt gttattttta tatattcttt ttaagaatga ttgttttttt atagaaattt
    14821 taaaaaataa aacacgttat tgatggatag ttagtttgtt atttggtaat attatagaag
    14881 ttgactggta gtgatttatt tttcgtcgtt ttataaaaat gttatttttt ggaaaaattc
    14941 acaaatatgt aacgaatgtt tttctggaat ttgctgtgtt aaaaagcagt atgactaata
    15001 taaatactat tgtcatttat gcaatttttt atggatagta attttagagg ggaatcaatg
    15061 aaggaagaat agaagggaaa aatagttgca aatacgccag ttagcaaaaa tagcaataca
    15121 tcttcaagct ttaaaattcg agagaatcat cttggtagtg gaatcaacta caacaaaagg
    15181 attaaaccaa ttaatagata gaattaccaa tggaaaaata gaaaaggtag ttattctata
    15241 caaaagttgt ttaattcgct tcggttatga acttagtgaa accctttgca atatatggta
    15301 cagaatatag aaattattga cattacagaa aatgatgaag atttaataca gattgtcact
    15361 atttgtagat accttttaca aggaaaccga gccaataaag ctaagaagat gattaaggag
    15421 ttgatggaag acgatacaag ttaagaaagt gaaactacgc ccaaataaag agcaaaaaga
    15481 gtaattggac gtggaaaatg caagaaatga accatcgatt cggtggacag tttattagta
    15541 atgacggttt gagaacatcc atgacgagaa tgaaggaaca tcctaaatgc gtttagttga
    15601 accaagtgcc aaataatatg gccaaataag cgatcagtta tcgatgaatc ccaagtattc
    15661 taaactatat attcattttg atgctaagca ttgatatatt tttttgggat tgatgaaggt
    15721 taaccaatac agggactcac tgatgaaatt ctagagtctg accttggtat caacgattta
    15781 gtgattgttt ctaatggaaa agtataaaag tatataagta cattaacaaa ataactatta
    15841 gggttacgct ccagcttgtg caaggcactg gcaggaggaa tcgtgaaatc ataattatgg
    15901 ttaagccaca cggacaacta aaagtcatac aaagctatcg aatcaatcaa cagaagacaa
    15961 tcggcaatca ttagctttgt ttacagctta cattgcaacg cttgagttga accgaaaata
    16021 cgagggaggt aacgcagcca ttttgagttg gggaatcttt ttagaaaatt atcgggtacc
    16081 ttgttgttta gtcaaaatta taagggggag taatagtgag gaacaaattt cataaactgt
    16141 taagtatgtt tctagttatg cttattgtga tcaatcaatt tgggaacata acggttctag
    16201 ctgagtcttt agcagaacag ccagagcgtt cgctgacgat cgaagttgat caagaggagg
    16261 ctgctttggg tgagcagttc cagttgcgag tcagaggaac accagacctg ctaaatgcat
    16321 tggtcattga taagagtgag gatttgattg aaggggaagt gaagcaactc aatgattacg
    16381 aacgtctgat tacttattct tcccagcatt caggtaaata cgaattgacc gcaagaagcg
    16441 gtgatattca atcaaatacg gtctcaatag aaatcagtgt accagaagaa agcacaagtg
    16501 agtcttttaa tgtaccagaa gaaagcacaa gtgaatcttt taatatacca gaagaaagct
    16561 taagtgaatc ttttaatgaa gccagatcat ctgagccatc tataacgcag gaatctcaga
    16621 ttgaaagaca agaggagctc ttgaattatg acatcggcat ctctgctgag cgccccactt
    16681 tgccgagcag gcatacacaa acgttaaagg gatacattca aggaggcgct ggcgatattc
    16741 ttgaagaata tggggagatg gtcgtacgag ttcctagagc atcgattggc ttaattgagt
    16801 tcattagagt ggaggtcgct gatgattcac ctttaaagcc actggcattt taccaagaag
    16861 gcacagacgt tgtttttcca tttacctttg attatgaagg attaaatgaa gccgctcagt
    16921 cgtatacatt cagtatcacg tttgaagttg ctttctttca atcaggcggt actgagccaa
    16981 ataatgactt tcaagtgagt cttacttatg ctgagcgagc agcaaacacg gaattttcaa
    17041 ttacgcctat tccttcatca agacccattt tttcgaaatt atactatggc tctagagaaa
    17101 tcgacagcca aggaaatctt gggaatctgc tgattgacgg gcaagacgtg accaacagta
    17161 ataaatttat tttgcaggta aattatgaag gatcacgaaa agacggtgta gtggttaccg
    17221 atgtactacc gcaaggaaca actttggcac cgaatccgac tacatggagt tccactggaa
    17281 atagagaacc tatttcaggt ggggttcgga tcgcagaagt aacgaatcca ggtgaagcaa
    17341 acgttagaac tcattatgtt tccgaggaat ttgtggacaa agtatctttt aataaggaaa
    17401 cgaatacact tgttgtggac tttggtaatc tagcagaggg tcaatcgtat tttattgaat
    17461 atgctcttga agtgaccgat gtcgacttgt tcaatgaagg aagacaagag gatgggtttt
    17521 ctcggaatcg agcgagtatg acctacaata atcaggtaat gagccgagac taccgcttga
    17581 gaatccctga aagtaatctt ccggcaatct ctttaagaaa aagtgtggac aaaaatgtta
    17641 tcaatctcaa tgaaaatgaa ctaacgtata ctctgcatgt ctcgatgttt tctggaacaa
    17701 gaaatacgct tgttattgaa gatgttttgg aagaaggcat ggaatttgtg tcatttctat
    17761 cagatattcc ctctttcatt gatgccgaag tcaataatgg aatagttcgt ttcagtgcga
    17821 ataatacgat tggtggtccg acaagtttta ccctctcttt taaggtcaat gtagaggcgt
    17881 atgcggtcgg cgatcaaatt ttaaactttg caacgcttgt gaatagtgac aatcgattga
    17941 atacgagtac tgtaagaaca aggaaaatcg atgggcgtat taaagtaatt aaaactgatg
    18001 agaatggcca aaggctggca ggagcaaaat ttgaaatttt atcaagcgat tcacaagtga
    18061 tacaagaagg tataacggca gaaaacggag aatttttaag tgaaccgctt cctgtaggta
    18121 gctatcaagt ccgagaaaca gaagcaccgg aagggtatct tttagatgcg acggtacatg
    18181 atgtttttat aaatgaatac tccatagaag aagtgacgtt aaccattgaa aacatccttg
    18241 atacgggtgg tgttgaacta attaaacatg ctgaaggcag tggtgaagtc cttcaaggag
    18301 ctgtttttga gttacagaac cgagaaggag aaacgctaca aaccggt
//
LOCUS       DACEKQ010000060        17238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEKQ010000060 DACEKQ010000000
VERSION     DACEKQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.064"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(97..1116)
                     /locus_tag="IW671_001838"
     CDS             complement(97..1116)
                     /locus_tag="IW671_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0898818.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IW671_001839"
     CDS             complement(1138..2145)
                     /locus_tag="IW671_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0898819.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IW671_001840"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IW671_001840"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0898820.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IW671_001841"
     CDS             complement(2796..5090)
                     /locus_tag="IW671_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0898821.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IW671_001842"
     CDS             5350..7515
                     /locus_tag="IW671_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898822.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IW671_001843"
     CDS             7819..8652
                     /locus_tag="IW671_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0898823.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IW671_001844"
     CDS             complement(8754..10454)
                     /locus_tag="IW671_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898824.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IW671_001845"
     CDS             10961..12121
                     /locus_tag="IW671_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0898825.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IW671_001846"
     CDS             12137..12580
                     /locus_tag="IW671_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0898826.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IW671_001847"
     CDS             complement(13063..13593)
                     /locus_tag="IW671_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0898827.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IW671_001848"
     CDS             13698..14249
                     /locus_tag="IW671_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0898828.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IW671_001849"
     CDS             complement(14402..14875)
                     /locus_tag="IW671_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0898829.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IW671_001850"
     CDS             15013..16485
                     /locus_tag="IW671_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0898830.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IW671_001851"
     CDS             16482..17192
                     /locus_tag="IW671_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0898831.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5356 a         3098 c         3544 g         5240 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgtaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactt gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tctgcatc
//
LOCUS       DACEKQ010000061        17005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEKQ010000061 DACEKQ010000000
VERSION     DACEKQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.065"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..124)
                     /locus_tag="IW671_001852"
     CDS             complement(<1..124)
                     /locus_tag="IW671_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0898832.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IW671_001853"
     CDS             complement(228..539)
                     /locus_tag="IW671_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0898833.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IW671_001854"
     CDS             complement(541..738)
                     /locus_tag="IW671_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0898834.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IW671_001855"
     CDS             complement(1132..2859)
                     /locus_tag="IW671_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0898835.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IW671_001856"
     CDS             complement(2852..4045)
                     /locus_tag="IW671_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0898836.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IW671_001857"
     CDS             complement(4232..4876)
                     /locus_tag="IW671_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0898837.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IW671_001858"
     CDS             complement(4970..5104)
                     /locus_tag="IW671_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0898838.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IW671_001859"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IW671_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0898839.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IW671_001860"
     CDS             complement(7235..7708)
                     /locus_tag="IW671_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0898840.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IW671_001861"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IW671_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0898841.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IW671_001862"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IW671_001862"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0898842.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IW671_001863"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IW671_001863"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0898843.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IW671_001864"
     CDS             complement(11755..12894)
                     /locus_tag="IW671_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0898844.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IW671_001865"
     CDS             complement(13203..13916)
                     /locus_tag="IW671_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0898845.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IW671_001866"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IW671_001866"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0898846.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IW671_001867"
     CDS             15105..16637
                     /locus_tag="IW671_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0898847.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IW671_001868"
     CDS             complement(16828..>17005)
                     /locus_tag="IW671_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0898848.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3494 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgaa aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACEKQ010000062        16818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEKQ010000062 DACEKQ010000000
VERSION     DACEKQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.066"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..454)
                     /locus_tag="IW671_001869"
     CDS             complement(<1..454)
                     /locus_tag="IW671_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0898849.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="IW671_001870"
     CDS             complement(559..1422)
                     /locus_tag="IW671_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0898850.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="IW671_001871"
     CDS             complement(1618..3717)
                     /locus_tag="IW671_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0898851.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IW671_001872"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IW671_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0898852.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="IW671_001873"
     CDS             complement(5070..6911)
                     /locus_tag="IW671_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0898853.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9486)
                     /locus_tag="IW671_001874"
     CDS             complement(7093..9486)
                     /locus_tag="IW671_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0898854.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="IW671_001875"
     CDS             complement(9593..10453)
                     /locus_tag="IW671_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0898855.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="IW671_001876"
     CDS             complement(10512..11033)
                     /locus_tag="IW671_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0898856.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11353..12072
                     /locus_tag="IW671_001877"
     CDS             11353..12072
                     /locus_tag="IW671_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0898857.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="IW671_001878"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="IW671_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0898858.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IW671_001879"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IW671_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0898859.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="IW671_001880"
     CDS             16432..16788
                     /locus_tag="IW671_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0898860.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5765 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaa
//
LOCUS       DACEKQ010000063        16726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEKQ010000063 DACEKQ010000000
VERSION     DACEKQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.067"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1241)
                     /locus_tag="IW671_001881"
     CDS             complement(39..1241)
                     /locus_tag="IW671_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0898861.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="IW671_001882"
     CDS             1358..1873
                     /locus_tag="IW671_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0898862.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="IW671_001883"
     CDS             complement(2072..2518)
                     /locus_tag="IW671_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0898863.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW671_001884"
     CDS             complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW671_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0898864.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="IW671_001885"
     CDS             complement(4397..5026)
                     /locus_tag="IW671_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0898865.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="IW671_001886"
     CDS             complement(5010..5474)
                     /locus_tag="IW671_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0898866.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="IW671_001887"
     CDS             complement(5961..7229)
                     /locus_tag="IW671_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0898867.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGFTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="IW671_001888"
     CDS             complement(7460..8197)
                     /locus_tag="IW671_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0898868.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="IW671_001889"
     CDS             complement(8264..8833)
                     /locus_tag="IW671_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0898869.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="IW671_001890"
     CDS             8961..9833
                     /locus_tag="IW671_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0898870.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="IW671_001891"
     CDS             10175..11047
                     /locus_tag="IW671_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898871.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="IW671_001892"
     CDS             11044..13002
                     /locus_tag="IW671_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0898872.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="IW671_001893"
     CDS             complement(13253..14104)
                     /locus_tag="IW671_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0898873.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="IW671_001894"
     CDS             14227..14694
                     /locus_tag="IW671_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0898874.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="IW671_001895"
     CDS             complement(14957..15439)
                     /locus_tag="IW671_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0898875.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="IW671_001896"
     CDS             complement(15539..16402)
                     /locus_tag="IW671_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0898876.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         4992 a         3470 c         2980 g         5284 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtaaaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactactt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttcttt
//
LOCUS       DACEKQ010000064        16529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEKQ010000064 DACEKQ010000000
VERSION     DACEKQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.068"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW671_001897"
     CDS             complement(142..417)
                     /locus_tag="IW671_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0898877.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW671_001898"
     CDS             complement(660..1970)
                     /locus_tag="IW671_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0898878.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW671_001899"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW671_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0898879.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW671_001900"
     CDS             complement(3516..4196)
                     /locus_tag="IW671_001900"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0898880.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW671_001901"
     CDS             complement(4245..4880)
                     /locus_tag="IW671_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0898881.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW671_001902"
     CDS             complement(4946..6349)
                     /locus_tag="IW671_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0898882.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW671_001903"
     CDS             complement(6336..7367)
                     /locus_tag="IW671_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898883.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW671_001904"
     CDS             7398..7619
                     /locus_tag="IW671_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0898884.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW671_001905"
     CDS             complement(7784..8521)
                     /locus_tag="IW671_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0898885.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW671_001906"
     CDS             complement(8674..10014)
                     /locus_tag="IW671_001906"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0898886.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW671_001907"
     CDS             complement(10251..11774)
                     /locus_tag="IW671_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0898887.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW671_001908"
     CDS             complement(12152..12763)
                     /locus_tag="IW671_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0898888.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW671_001909"
     CDS             complement(13093..13809)
                     /locus_tag="IW671_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0898889.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW671_001910"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW671_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0898890.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW671_001911"
     CDS             complement(14368..15165)
                     /locus_tag="IW671_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0898891.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMVIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW671_001912"
     CDS             complement(15135..15506)
                     /locus_tag="IW671_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0898892.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW671_001913"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW671_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0898893.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4646 a         3577 c         2797 g         5509 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattacca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttcttttt
//
LOCUS       DACEKQ010000065        16510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEKQ010000065 DACEKQ010000000
VERSION     DACEKQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.069"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            184..1182
                     /locus_tag="IW671_001914"
     CDS             184..1182
                     /locus_tag="IW671_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0898894.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="IW671_001915"
     CDS             1294..1803
                     /locus_tag="IW671_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0898895.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="IW671_001916"
     CDS             complement(1833..2492)
                     /locus_tag="IW671_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0898896.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="IW671_001917"
     CDS             complement(2494..3258)
                     /locus_tag="IW671_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0898897.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="IW671_001918"
     CDS             complement(3266..3943)
                     /locus_tag="IW671_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0898898.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="IW671_001919"
     CDS             complement(3980..5365)
                     /locus_tag="IW671_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0898899.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="IW671_001920"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW671_001920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IW671_001921"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="IW671_001921"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0898900.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="IW671_001922"
     CDS             6304..7161
                     /locus_tag="IW671_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0898901.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="IW671_001923"
     CDS             complement(7242..7577)
                     /locus_tag="IW671_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898902.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="IW671_001924"
     CDS             complement(7644..9014)
                     /locus_tag="IW671_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0898903.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="IW671_001925"
     CDS             complement(9092..9628)
                     /locus_tag="IW671_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0898904.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="IW671_001926"
     CDS             9937..11418
                     /locus_tag="IW671_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0898905.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11521..12228)
                     /locus_tag="IW671_001927"
     CDS             complement(11521..12228)
                     /locus_tag="IW671_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0898906.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12307..12645)
                     /locus_tag="IW671_001928"
     CDS             complement(12307..12645)
                     /locus_tag="IW671_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0898907.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12756..13718)
                     /locus_tag="IW671_001929"
     CDS             complement(12756..13718)
                     /locus_tag="IW671_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0898908.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13733..15064)
                     /locus_tag="IW671_001930"
     CDS             complement(13733..15064)
                     /locus_tag="IW671_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0898909.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15072..15752)
                     /locus_tag="IW671_001931"
     CDS             complement(15072..15752)
                     /locus_tag="IW671_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0898910.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15971..16450)
                     /locus_tag="IW671_001932"
     CDS             complement(15971..16450)
                     /locus_tag="IW671_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0898911.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
BASE COUNT         4884 a         3441 c         2737 g         5448 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    11461 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    11521 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    11581 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    11641 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    11701 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    11761 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    11821 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    11881 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    11941 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    12001 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    12061 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    12121 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    12181 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    12241 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    12301 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    12361 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    12421 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    12481 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    12541 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    12601 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    12661 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    12721 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    12781 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    12841 ttcgcaccac ttgctaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    12901 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    12961 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    13021 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    13081 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    13141 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    13201 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    13261 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    13321 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    13381 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    13441 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    13501 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    13561 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    13621 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    13681 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    13741 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    13801 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    13861 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    13921 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    13981 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    14041 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    14101 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    14161 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    14221 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    14281 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    14341 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    14401 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    14461 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    14521 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    14581 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    14641 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    14701 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    14761 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    14821 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    14881 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    14941 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    15001 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    15061 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    15121 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    15181 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    15241 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    15301 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    15361 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    15421 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    15481 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    15541 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    15601 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    15661 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    15721 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    15781 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    15841 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    15901 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    15961 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    16021 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    16081 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    16141 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    16201 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    16261 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    16321 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    16381 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    16441 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    16501 ttcaaaaatt
//
LOCUS       DACEKQ010000066        16460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEKQ010000066 DACEKQ010000000
VERSION     DACEKQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.070"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            346..3180
                     /locus_tag="IW671_001933"
     CDS             346..3180
                     /locus_tag="IW671_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0898912.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3371..3862
                     /locus_tag="IW671_001934"
     CDS             3371..3862
                     /locus_tag="IW671_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0898913.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3965..4954
                     /gene="manX"
                     /locus_tag="IW671_001935"
     CDS             3965..4954
                     /gene="manX"
                     /locus_tag="IW671_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0898914.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4987..5790
                     /locus_tag="IW671_001936"
     CDS             4987..5790
                     /locus_tag="IW671_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0898915.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5811..6725
                     /locus_tag="IW671_001937"
     CDS             5811..6725
                     /locus_tag="IW671_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0898916.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6808..7182
                     /locus_tag="IW671_001938"
     CDS             6808..7182
                     /locus_tag="IW671_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0898917.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7321..7566
                     /locus_tag="IW671_001939"
     CDS             7321..7566
                     /locus_tag="IW671_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0898918.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7738..8802
                     /locus_tag="IW671_001940"
     CDS             7738..8802
                     /locus_tag="IW671_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0898919.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9048..9473
                     /locus_tag="IW671_001941"
     CDS             9048..9473
                     /locus_tag="IW671_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0898920.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9466..9915
                     /gene="nusB"
                     /locus_tag="IW671_001942"
     CDS             9466..9915
                     /gene="nusB"
                     /locus_tag="IW671_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0898921.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10012..10866
                     /locus_tag="IW671_001943"
     CDS             10012..10866
                     /locus_tag="IW671_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0898922.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10867..12204
                     /locus_tag="IW671_001944"
     CDS             10867..12204
                     /locus_tag="IW671_001944"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0898923.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12209..12433
                     /locus_tag="IW671_001945"
     CDS             12209..12433
                     /locus_tag="IW671_001945"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0898924.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12435..13325
                     /locus_tag="IW671_001946"
     CDS             12435..13325
                     /locus_tag="IW671_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0898925.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13334..14152
                     /locus_tag="IW671_001947"
     CDS             13334..14152
                     /locus_tag="IW671_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0898926.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14259..14708
                     /locus_tag="IW671_001948"
     CDS             14259..14708
                     /locus_tag="IW671_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0898927.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14726..16408
                     /gene="recN"
                     /locus_tag="IW671_001949"
     CDS             14726..16408
                     /gene="recN"
                     /locus_tag="IW671_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0898928.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
BASE COUNT         5440 a         2731 c         3704 g         4585 t
ORIGIN      
        1 aaataaaagt tgtagtggag gctacgaagt ttactttgtc tttttacact taatattttt
       61 ttgccaattt ggaatcagga tgttttgata actatcgttt tcaattgatg gaagctatat
      121 tgtttgctat cagtgaaata attatatggt aaacagtgtt ctttttcttg tttccattat
      181 aaaaatcgta tcgggatttt tcgttctttt aaattgaaca tttctatcta tccgcgtgtt
      241 atcaacaaaa caaaatcagg taaatagttg acgaaacaga aataaataca tagtattatg
      301 ttttgaagcc taagtacaat agtctataga aaaggagcga taccattggc aaaacgcatt
      361 gaccgaattt atatgtatgt caaagaaaaa accgctcatt taacaaaggc agaatatgat
      421 caaggagtaa caacacagga aattgctgaa gtgttaggga ttcagcgaac aaatagcagt
      481 aaagatctga atcagctagt gcgcgaagga aaactgataa agacagacgg acgaccagta
      541 cgttacatat atcaaacact cgttacgcac gtaaagccag ttacaaaaca tgtagtcagt
      601 tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc aaatagatac taaggatatc
      661 tttgcaaaaa tcattggagc taacggcagc atgaaaaatt cagtggaaca ggcaaaagct
      721 gcgattttgt atccgccaag aggattaaat tgtttgatta caggacctac cggatcagga
      781 aagacttatt ttgcacacgc gatgtatcat tttgcaaaag cgcatcatgt aatagatgag
      841 gaaacggaac tgattgtttt caattgtgct gattatgcca ataaccctga gcttttgatg
      901 agtcatttat ttgggtacgc aaaaggtgct tttacaggcg cagatgaaga aaaaatgggg
      961 attatcgatc aagcggatgg cggtatgctg tttttggatg agattcaccg gttgccgcct
     1021 gaaggtcaag aaatgatctt ttactttatg gatcatggaa cttacagtcg tcttggggaa
     1081 acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg cgaccacaga agacccaggt
     1141 tcttctctat tagaaacttt tgttcggcgt atcccaatca atatcaaact tccggctttt
     1201 gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa tcatgattgc ccacgaagca
     1261 aaccgcacgc agcgaaagat ttctttgaca gaagatgtag caaaagcatt gatcggcagt
     1321 gtgacctatg gaaatatagg acaattgaag tcaaatatcc agttagtatg tgcacgagga
     1381 tttctaaatc atatgcaatc acccgaaatc tcgatcacaa tcgatgattt gacagaaggg
     1441 attcgtagcg gattgattca attagcaagc aaccgcagtg caatggcaga gctttctaaa
     1501 atattggagc caaagataac tgtagcacca aatgatacga tcatgaaaat ccaatcggac
     1561 tcctatgagt taccttataa tctatatgat atcatcggag ataaagcagc tttactgaag
     1621 tcggatggtt tggatcaaga agcaatcaat catttcattt ctacagatat caacatccat
     1681 ctgaagtctt tctataaaga ccatggtttt tcttttaatg cagacagcaa attagcagaa
     1741 tttgtagatc cgaaaatcat agaagtgacc aatcagattt atgtaatggt caaaagatct
     1801 ttaccatatg agtttcagca aaactttatt tatgctatga gcttgcatat cagttctttt
     1861 ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg ataacatccg tgagatggct
     1921 tttgattttc cagatgaata tgctgttgcg aaagaagtgc gtagttatat cgaaacgtat
     1981 tttcaagtgg agattccaga aagtgaagac tattacttga ccgttctact cgtctcgtta
     2041 cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg cagctcacgg aaacagcaca
     2101 gcaagcagta tggtccaagt tgtccaacag ttgttagatg cagagaacgt acgagcagta
     2161 gacatgcctt tggatatgga tccgaaaaca gcattggacc gaattgaacg caatgtacaa
     2221 gaagtagatg aaggaagcgg ggtcatactc ttagtagata tggggtccct tgcgtcgttt
     2281 aattcgcaaa ttcaaagaga cactggaatt ccagtacgta ctgttgatat ggtaacaact
     2341 tctctagttt tggaaactgt acgaaaggtc tcagtattag ggacagatct agatatgttg
     2401 catgaatcat tgaaaaattt tcgaggttac gcagaaatat cttctgtaga atctgttcag
     2461 gaagcaaaac ctattcataa gaaaaaagct gttttggcta tctgcgcatc tggcgaaggt
     2521 acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa acaagcgtca agaaacagaa
     2581 ttagaagttc tagctttgtc gattgtggaa gtgaaagaag aactgccagt tatccaaaaa
     2641 gaatatcaga tcatagctac tactgggatt actgatttga atatccaagc cccatttatt
     2701 cctttagaac gttttattga tcaaaatatc gaagtcattt tggatcagtt gctgatggaa
     2761 tcagaattgg aggaaactga attcatctcc ctagatgaag agtcagctaa aaatacgtgt
     2821 gttgagttta tctctgataa cttcatcttt attaatggta gtaagttaat cgatccaatg
     2881 tggcaatttt ctacacaaat ctctcagacg acgggaatcg gcgatgaaga atatggattc
     2941 aaaatcaacc tagtgatgca cacagcagga atgatcgaac gtattattcg gaatgaacca
     3001 ttgactgtgg aagaaaacga attgacaaat acaacgaatg atcctcttta tagccaattg
     3061 gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa aagtgccgat agaagaaatg
     3121 tattatctgc tacgtctagt ccataatcag ttagataaaa aagaatacac tgtgccatga
     3181 atacacaaaa acgatacact tttatagtgt atcgtttttg tgtattggtt aagaatcctt
     3241 taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc
     3301 taggaggtgg ttgttcacac agaaatgata ggggtttctg tgaagtagtg aggaagaagg
     3361 agttttgcag atggatattc gattggcacg tatagatgat cgtttaatcc acgggcaagt
     3421 cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt
     3481 cgctcaagat aaattgagaa agtttctact aaaagaagct gcaccgccgg ggatcacagc
     3541 aaatgtaata accgtgcaga aaatgattca aatctatcat agtggattat tatcacgagc
     3601 aaaagtgatg cttttattca ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt
     3661 acagttgaag tcgttgaata tcggcggtat gagttttact gaaggcaaat caatgattac
     3721 caattttgtt tcagtaaatc aacaagatat tgattccttt cgttacttga acagtcaaga
     3781 agtggagtta gagattcgaa aagtacctgc tgacaggaaa atccttttga tggaactact
     3841 agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa aaggtcaaat aattctgttg
     3901 taatggttag caattattaa tatttttgaa tttgttacaa caaaagtaaa taggaggtat
     3961 aaatatggta gggattatcc tagcaagtca tggacaattt gctgaaggaa tcttacaatc
     4021 cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga
     4081 aggtccagat gacttgagag ccaaattgga agaagcggtt gcttcttttg ataatcaaga
     4141 cgaagtgtta ttcttagttg atttatgggg cggaacaccg ttcaatcaat caaacacatt
     4201 attcgaagag cacaaagata aatgggcaat cgtcagcggt ttgaatttac caatgttgat
     4261 tgaagcctac gcatcacgct tttcaatgga atctgctcat gaaattgcag cacacatcat
     4321 tgaaacagca aaagacggtg taaaagtaaa accggaagaa ttagaaccgg cagaagcacc
     4381 gaaagcagca gttgaagacg cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg
     4441 agacggaaaa atcaaatatg tattagcacg tgttgactca cgtctgttac atggacaagt
     4501 tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt
     4561 atcaaaggat gatctgcgta agagactaat cgaacaagct gctcctccag gtgtaaaagc
     4621 aaatgttatt cctattagca aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac
     4681 caaagcattg cttttatttg aaaatcctga agacgtattg acagctgttg aaggtggcgt
     4741 agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag
     4801 taaagtattg tctatgggac aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg
     4861 tgtgaaattc gatgtacgta aagtacctaa tgattcacgc gataacatgg atgatatttt
     4921 gaaaaaagca aaagctgaat tagcaaaagc gtaataaata atattaaaat ttataggagg
     4981 cttatcatgt ctattatttc aataatttta gtcgtattag ttgcctttct agctggtatg
     5041 gaaggtattc ttgatgaatt tcaatttcat caaccactag tggcatgtac attgatcggt
     5101 ttagttaccg gtaacctcga agctggtatc gtacttggcg gtacgcttca gatgatcgct
     5161 ttaggttggg caaatatcgg tgccgctgta gcaccagatg ctgcattagc atcagtagca
     5221 tcagcaatta ttttagtttt aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg
     5281 attgccgttc ctttggctgt tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct
     5341 gttccaatcg ttcatttgat ggatgcggct gctgaagaag gaaacatccg taaggttgaa
     5401 atgtggcaca tcattgctgt atgtttacaa ggggttcgta tcgcggtccc agcagcagcg
     5461 ttgctattta tcccagcaca aactgtgcaa tcattccttg aatcaatgcc agcatggtta
     5521 acagatggta tggctatcgg tgggggaatg gtcgtagctg taggttacgc tttggtaatc
     5581 aacatgatgg ctacaaaaga agtatggcca ttcttcgtta ttggtttcgt tgtagctgca
     5641 atttctcaat taacattgat tgctctaggt gctttaggtg ttgcattagc tcttatttac
     5701 ttgaaccttt ctaaaatggg tggttcatca aataatggag gcggaagcaa ctctggtgac
     5761 cctttaggcg acattttgaa tgactattaa tcttgaagga ggaaaataaa atggcagaag
     5821 aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc gtggcgctct accttcattc
     5881 aaggttcatg gaactacgaa cgtatgcaaa acggtggctg ggcattttct atgattccag
     5941 caatcaaaaa attatacaaa acaaaagaag aacgttctgc agcattaaaa cgtcacttgg
     6001 aattctttaa tacacaccca tacattgctt ctccaatttt aggggttaca ttggctctgg
     6061 aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc gattcaaggg gttaaagtag
     6121 gtatgatggg tcctttagct ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa
     6181 tgctaggtgc actaggtgct tcattagcta tgggaggaaa tatccttgga ccaatcatct
     6241 tcttcttagg ttggaacatt attcgttggg cattcatgtg gtatacacaa gaatttggct
     6301 acaaagctgg ttctaaaatt actgatgacc tttctggcgg attgttacaa gacgtaacaa
     6361 aaggtgcttc aattttaggg atgttcgtat tagctgccct tgtacaaaga tgggtatcaa
     6421 tcaaattctt accagttgta tcaactgtaa aattagataa aggtgcttac atcgaatggg
     6481 ataaattgcc ttcaggtggt gaaggaatca agagtgcatt tgaacaagtt aataatggct
     6541 tagccctttc tcctgaaaag gtaacaacat tacaagacaa cttagaccaa ttgattcctg
     6601 gtttagctgc attaggatta acattgttat gtatgtggtt gcttaagaaa aaggtttctc
     6661 caatcgttat catccttggt ttattcgtag tcggtgttgt tggacacgtc atcggacttt
     6721 tataatccat aaaaatatag ctcgggcgtt gtcccgagct ttttttatat agtatactgt
     6781 tcataaacaa atatctggag ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg
     6841 gtaattgatg ctacttcgtt tacgggtctg acagattacg ggcaaatcat gataggtgat
     6901 aatggttttg agttttacca tgcgcgagat agacgcaaat atatccaaat tccatgggaa
     6961 gaagtggatt atgtactagc atcagtcatg ctaaagggaa aatggattcc tcgatatgcc
     7021 attcaaacga aaaaaaatgg tacatacaca ttttcttcca aagaacctaa aaaagtgctt
     7081 cgtgcgatca gagaatatgt agatgaagat agaatggtac aatcattgag tttctttgat
     7141 gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct
     7201 aagaggaggt tttctttatt tttttacttt gcagaagaaa atatgaaata gcaaaaccaa
     7261 ttatttgtcc cctttaaatc ccatatgcta aaattgatta gcaaagaagg aggttatatt
     7321 atgagttggt tatggacatt gattgttgga gccgtgatcg gagcaattgc aggtgcgatc
     7381 accagtaaag gaaattcgat gggctgtgta acgaatatca ttgctggact agtcggttca
     7441 agtattggac aagcgatttt cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt
     7501 ccatctattt taggcgcagt gattttggta gcagttgtct catttttctt tggaaaacga
     7561 tcataagttt ttacaaaata gtaaactaaa aggagttgca gcgagactgt gactcctttt
     7621 tggctttatt tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt
     7681 acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg
     7741 atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg
     7801 gttaccagtc cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct
     7861 gttattactt tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca
     7921 gcgcaggctc aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca
     7981 aacttagtac gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt
     8041 ttagaatatt cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg
     8101 atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt
     8161 catattgcgg atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag
     8221 attgaagtcg caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct
     8281 tttgatacga tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa
     8341 aaaatcatcg aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac
     8401 gtttccgata tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt
     8461 tatcaaatag tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact
     8521 ggtgtccaat tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca
     8581 tttggacatt ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct
     8641 tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac
     8701 ttaccaggaa tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat
     8761 cgtgtgttga ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag
     8821 taagcgtaaa aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac
     8881 actatagaag aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat
     8941 agcagtttgc agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata
     9001 gagaattcgg atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa
     9061 aaaatttgat tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca
     9121 tcgaagtaat tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa
     9181 cttttgccag caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact
     9241 tagcaaacga ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt
     9301 cagttcctaa agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga
     9361 cggatatcga tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag
     9421 aacaaccaac aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta
     9481 tcaagacacg agattcggga aatggcattg caggctttat ttccattaga cttcaatgca
     9541 gacctaacaa aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc
     9601 aacgaagatg aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt
     9661 gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc
     9721 aatcggatct caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat
     9781 gtgacagatg taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt
     9841 agtgatgacc gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta
     9901 gaagctgggg cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg
     9961 ggaaaattct atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa
    10021 ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa
    10081 aaattgaaag ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca
    10141 gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca
    10201 ttggtagagc gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa
    10261 tacaatcaag atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata
    10321 gatgaagaaa agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg
    10381 atgaatttag gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc
    10441 atcatgaaaa tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc
    10501 ggacgaagca atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact
    10561 gttactgttg ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt
    10621 ttagtcgtag caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct
    10681 gtagtgatag atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa
    10741 tttgatgaag tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca
    10801 atgactatca cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt
    10861 gaatagatgg cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa
    10921 tttgatgccg atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt
    10981 ttacgtgcca atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct
    11041 attatgttca aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc
    11101 cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag
    11161 cacatggagc ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag
    11221 ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga
    11281 atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa
    11341 agaagatatc caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca
    11401 gcggacaatg ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg
    11461 atcatcggtc gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt
    11521 gctcgggcga tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat
    11581 accacgatcg ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa
    11641 ttagctgttc ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg
    11701 cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt
    11761 tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag
    11821 ttgaaacagc gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg
    11881 atcgcattaa gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc
    11941 caagaaactg cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa
    12001 cagcagcagc gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc
    12061 atgggaagag gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag
    12121 attcatcaag gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta
    12181 caagagatag acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca
    12241 agaattagaa aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct
    12301 agattcgttt aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc
    12361 agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa
    12421 cgaggaaagc tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa
    12481 aagaaatctt gtctttttta gatgagcata caacggatag aggactctat gatgcgatga
    12541 gttattcagt gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg
    12601 cttcctttga tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg
    12661 tgcatacgta ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta
    12721 gaggaaaacc taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg
    12781 gtttgcttac aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc
    12841 ttctgctgct tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag
    12901 ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg
    12961 aaagaaagac agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc
    13021 aacctgaaga aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc
    13081 agataagaga tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg
    13141 gtaaagatga acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac
    13201 gtgaggcgtt ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg
    13261 acgttttttc ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga
    13321 aatgagggaa agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt
    13381 tgaaacaaga gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa
    13441 cgagcggttt gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg
    13501 taaaaaactt ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt
    13561 tgatctatct gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac
    13621 tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca
    13681 gctagcttgg aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg
    13741 ttatgcaaaa cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc
    13801 ttttatttca ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga
    13861 agtgattgct ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg
    13921 aattgttcgt gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc
    13981 acaaggtgga tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa
    14041 tatcgagttc ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat
    14101 taatatggca gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac
    14161 tgtgaaaatc aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat
    14221 cattgattaa gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc
    14281 gtttgattac tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa
    14341 tcctgaaaag caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac
    14401 tgaaactgat caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa
    14461 caaacgaaga tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg
    14521 accaaatgga aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa
    14581 atttgattga taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg
    14641 ttttgatgat tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt
    14701 acctttaagt tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat
    14761 tattccggaa ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg
    14821 tgcgggtaaa tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag
    14881 cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa
    14941 acaagaagga ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct
    15001 gattgttcga cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat
    15061 tatcacatta gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa
    15121 cgaacatcag gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga
    15181 tgtagctttc caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct
    15241 ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat
    15301 gctccatttc cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa
    15361 attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc
    15421 aggttacgga gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt
    15481 ttcagaactg caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat
    15541 ccaaagtgct tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt
    15601 attggagtta gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca
    15661 gttgaaacgt aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac
    15721 cgaagagcta gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact
    15781 agagcaaaaa gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa
    15841 aatcgcaaaa gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa
    15901 cactgagttt gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt
    15961 tgaccaaatc gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa
    16021 agtcgcttct ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc
    16081 tgagcaaggt gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt
    16141 tgcacaggcc atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat
    16201 cactcatctg cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt
    16261 gattgatgga cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga
    16321 aattgcacgt atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga
    16381 actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc
    16441 agctgatagc tatattttat
//
LOCUS       DACEKQ010000067        16190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEKQ010000067 DACEKQ010000000
VERSION     DACEKQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.071"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..447
                     /gene="scm"
                     /locus_tag="IW671_001950"
     CDS             <1..447
                     /gene="scm"
                     /locus_tag="IW671_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0898929.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSA
                     SSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTS
                     NDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(602..1588)
                     /locus_tag="IW671_001951"
     CDS             complement(602..1588)
                     /locus_tag="IW671_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0898930.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1611..2177)
                     /locus_tag="IW671_001952"
     CDS             complement(1611..2177)
                     /locus_tag="IW671_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0898931.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2191..2607)
                     /locus_tag="IW671_001953"
     CDS             complement(2191..2607)
                     /locus_tag="IW671_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0898932.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3064..4998)
                     /locus_tag="IW671_001954"
     CDS             complement(3064..4998)
                     /locus_tag="IW671_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0898933.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5300..6235
                     /locus_tag="IW671_001955"
     CDS             5300..6235
                     /locus_tag="IW671_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0898934.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6325..7428
                     /locus_tag="IW671_001956"
     CDS             6325..7428
                     /locus_tag="IW671_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0898935.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7441..8394
                     /gene="menA"
                     /locus_tag="IW671_001957"
     CDS             7441..8394
                     /gene="menA"
                     /locus_tag="IW671_001957"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0898936.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9208..10554)
                     /gene="gor"
                     /locus_tag="IW671_001958"
     CDS             complement(9208..10554)
                     /gene="gor"
                     /locus_tag="IW671_001958"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0898937.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10688..11929)
                     /locus_tag="IW671_001959"
     CDS             complement(10688..11929)
                     /locus_tag="IW671_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0898938.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11947..12870)
                     /locus_tag="IW671_001960"
     CDS             complement(11947..12870)
                     /locus_tag="IW671_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0898939.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13091..15583)
                     /locus_tag="IW671_001961"
     CDS             complement(13091..15583)
                     /locus_tag="IW671_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0898940.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15590..16057)
                     /locus_tag="IW671_001962"
     CDS             complement(15590..16057)
                     /locus_tag="IW671_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0898941.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4756 a         3384 c         2884 g         5166 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagcacg
      121 gacacgtcag cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat
      181 tcatcaactt caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca
      241 agtgtctcct tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa
      301 acaagcaatg acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg
      361 aataattgga taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt
      421 atgagttata agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc
      481 taacggacgt tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt
      541 tgtcaaaaaa cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta
      601 ttcagtcttt gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa
      661 gagaggtggc aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta
      721 acttcctagc ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat
      781 cttcttctgt cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt
      841 gcaaagagta gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt
      901 tgccgatttc ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca
      961 tcccgataga aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt
     1021 caaaagcgcc aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt
     1081 ccaaatattc ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc
     1141 cattcaatac tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt
     1201 aatcagacaa tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga
     1261 cattaccaaa ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga
     1321 tatcatcatg gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg
     1381 ctttgtctcg atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca
     1441 acagctttcc gccggcatgg atcgtttctt tgatggcttg ttcgacaggt ttgtttttca
     1501 ggcggacagt ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat
     1561 agttttccca cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg
     1621 gtaaacattg ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga
     1681 agtgtctgca catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa
     1741 atccctaaaa ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa
     1801 gaagccatca aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg
     1861 atactgacac gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa
     1921 gcaccactca tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat
     1981 agtcgtagcc aaagtagtaa gaagctgtct tgttttttca tggtaaaaat ggcaacgatc
     2041 gtgattagat tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc
     2101 atattttcca ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt
     2161 ttttgtaatt tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc
     2221 atctgttgaa cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt
     2281 tttcgttgcc cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg
     2341 tactcgatcg ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata
     2401 tgtgttgctc tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg
     2461 cagtaccgct tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg
     2521 taaaaaagaa agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact
     2581 tttttcagca aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca
     2641 attttagcat aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga
     2701 agaatagaaa acagctttcc atgttttcta attctttggg cttattaccg agaagcagca
     2761 ggtggaacac cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga
     2821 aggaatttac gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa
     2881 gctacttctg gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc
     2941 ttgctcatgg aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct
     3001 gtgacggttt gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc
     3061 tatttatttc ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg
     3121 ttgtagccat ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa
     3181 tgcgaaagca acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa
     3241 cgtgatcacc aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa
     3301 tgctaatgcc aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc
     3361 attgtttggc atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac
     3421 agccattggt tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc
     3481 ttctccggca cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc
     3541 accactggca acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt
     3601 gaaccatgat ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat
     3661 ttttttggaa gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca
     3721 taagacattg ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg
     3781 gaaaaactca tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta
     3841 tttcaaattc acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc
     3901 aaataggtta gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc
     3961 tttaaattta tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg
     4021 tcctgtttgt tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata
     4081 aaagactaaa tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata
     4141 ttcattggcg atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga
     4201 agttcctttg acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt
     4261 caatttgata tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt
     4321 gtgcttacgc aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat
     4381 cgtcggcatt gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt
     4441 ggctaggcga tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga
     4501 accacagaca acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc
     4561 ttttgctact gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag
     4621 ggtgaatccg tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc
     4681 aaggatcagc tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt
     4741 atcgataccg attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg
     4801 ctgcagatcg tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc
     4861 ggtcatcata gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt
     4921 cagtttcttt gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac
     4981 aatattttgc tttgccattg atcatccgct ccttatagac tatctatata atgaatatat
     5041 gataattctt ttagtttgat acttattaca caatctccat atattatata gatgttctgt
     5101 tcttttgtct aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga
     5161 aatttgctga aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact
     5221 tataggctaa aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat
     5281 ggaatggtgg ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc
     5341 attttcagct ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc
     5401 ttctgaatca tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa
     5461 agttgtagct ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc
     5521 aaatggttac cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa
     5581 atatgataac gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa
     5641 catggaagca aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt
     5701 cttaaaagca caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga
     5761 aagcttccaa aactacgcac aacaattgat ccaagctgct caagctggta acacagatac
     5821 aatcgaaatc gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa
     5881 ctactctaac ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga
     5941 atcaaacttt gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa
     6001 tgaaaatatg aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga
     6061 cgaattcgtc aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac
     6121 aggtgctaca cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc
     6181 tgaaaaaggc gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca
     6241 taatcatact aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc
     6301 tttcaaaatg aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg
     6361 ggcatctctt ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa
     6421 agttcagcag ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg
     6481 acttacgttc gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc
     6541 gatcgagtaa aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat
     6601 gaagtgaatg agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta
     6661 gtcaagcggg cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt
     6721 gcaatcacac aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa
     6781 atcgatcagg cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa
     6841 actgtttatc taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac
     6901 attacggacg aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac
     6961 ttgggcggta atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca
     7021 gtcggcatcc aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg
     7081 aacaaaacat tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa
     7141 taccatcatt tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc
     7201 acgatcataa cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg
     7261 ggtgtgaaaa aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc
     7321 ttcgtgacaa aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta
     7381 tcggaggact cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc
     7441 atgacactaa aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt
     7501 ccttttgcgg taggggtatt attctctatc gctttttttc atgagataca ttggctaaac
     7561 acttttttat tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat
     7621 tacatggatt atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc
     7681 cgcagaaatc tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt
     7741 tttgttatcg gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt
     7801 atctgttgtt ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct
     7861 ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat
     7921 gtcaatacat tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt
     7981 gatttggtag gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg
     8041 tttacgattg ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa
     8101 aatcatcgct atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag
     8161 ctattgatgt atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa
     8221 tggccgatct tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc
     8281 aagcaaacgt taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc
     8341 aatgccagtt atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat
     8401 gaagaggttg tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc
     8461 tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
     8521 tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     8581 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
     8641 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
     8701 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
     8761 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
     8821 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
     8881 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
     8941 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
     9001 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
     9061 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
     9121 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
     9181 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
     9241 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
     9301 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
     9361 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
     9421 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
     9481 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
     9541 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
     9601 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
     9661 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
     9721 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
     9781 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
     9841 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
     9901 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
     9961 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
    10021 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
    10081 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
    10141 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
    10201 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
    10261 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
    10321 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
    10381 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
    10441 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
    10501 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
    10561 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
    10621 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
    10681 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
    10741 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
    10801 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
    10861 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
    10921 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
    10981 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
    11041 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
    11101 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
    11161 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
    11221 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
    11281 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
    11341 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
    11401 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
    11461 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
    11521 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
    11581 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
    11641 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
    11701 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
    11761 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
    11821 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
    11881 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
    11941 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
    12001 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
    12061 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
    12121 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
    12181 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
    12241 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
    12301 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
    12361 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
    12421 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
    12481 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
    12541 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
    12601 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
    12661 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
    12721 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
    12781 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
    12841 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
    12901 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
    12961 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
    13021 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
    13081 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
    13141 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
    13201 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
    13261 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
    13321 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
    13381 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
    13441 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
    13501 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
    13561 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
    13621 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
    13681 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
    13741 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
    13801 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
    13861 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
    13921 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
    13981 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
    14041 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
    14101 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
    14161 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
    14221 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
    14281 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
    14341 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
    14401 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
    14461 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
    14521 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
    14581 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
    14641 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
    14701 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
    14761 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
    14821 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
    14881 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
    14941 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
    15001 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
    15061 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
    15121 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
    15181 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
    15241 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
    15301 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
    15361 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
    15421 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
    15481 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
    15541 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
    15601 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
    15661 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
    15721 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
    15781 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
    15841 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
    15901 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
    15961 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
    16021 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
    16081 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
    16141 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACEKQ010000068        14835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEKQ010000068 DACEKQ010000000
VERSION     DACEKQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.072"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW671_001963"
     CDS             complement(<1..563)
                     /locus_tag="IW671_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0898942.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW671_001964"
     CDS             complement(750..1400)
                     /locus_tag="IW671_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898943.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW671_001965"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW671_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0898944.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW671_001966"
     CDS             complement(2912..3910)
                     /locus_tag="IW671_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0898945.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW671_001967"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW671_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0898946.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW671_001968"
     CDS             complement(4926..5657)
                     /locus_tag="IW671_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0898947.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW671_001969"
     CDS             complement(5661..6926)
                     /locus_tag="IW671_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0898948.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW671_001970"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW671_001970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0898949.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW671_001971"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW671_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0898950.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW671_001972"
     CDS             12268..13770
                     /locus_tag="IW671_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880041.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0898951.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW671_001973"
     CDS             13772..14509
                     /locus_tag="IW671_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0898952.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         4356 a         3186 c         2498 g         4795 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccagaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaacg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaactcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ccacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgaacc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttatta tgagcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttacgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcgaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttatcaaaga atatgtagcg
    13081 gatggttcag aaacttctag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccac aagcaatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacatttt tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc agcactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta catctgcact
    14281 cgaccccgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg cttcgctaaa gaagtggcca atcgtgtcat
    14401 gttcattgac gacggaaact ttttagaaga cggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtattg aatatttaat aaaaaccaaa
    14521 aatgcccggt tgcttcatga cgaccgggca tttttagttt cttgtttttt cacttaactg
    14581 taccagccgc atcgcttgtt ctcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgacgat atcaagtaca tagtcgatac caccttcaga
    14701 aactatgatg attatcttat tctaaagctg gcttttttgt tggtacaaac atgacaacta
    14761 caaaaaaacc aggcgaacgt atgattgttc gtctggtttt taattattaa tgacttagcg
    14821 tttttactag aaata
//
LOCUS       DACEKQ010000069        14578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEKQ010000069 DACEKQ010000000
VERSION     DACEKQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.073"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1421)
                     /gene="nhaC"
                     /locus_tag="IW671_001974"
     CDS             complement(3..1421)
                     /gene="nhaC"
                     /locus_tag="IW671_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0898953.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1824..3047)
                     /locus_tag="IW671_001975"
     CDS             complement(1824..3047)
                     /locus_tag="IW671_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0898954.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3078..3917)
                     /locus_tag="IW671_001976"
     CDS             complement(3078..3917)
                     /locus_tag="IW671_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0898955.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3934..4614)
                     /locus_tag="IW671_001977"
     CDS             complement(3934..4614)
                     /locus_tag="IW671_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0898956.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4616..5689)
                     /locus_tag="IW671_001978"
     CDS             complement(4616..5689)
                     /locus_tag="IW671_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0898957.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6336..7007)
                     /locus_tag="IW671_001979"
     CDS             complement(6336..7007)
                     /locus_tag="IW671_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0898958.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7094..9772)
                     /gene="mgtA"
                     /locus_tag="IW671_001980"
     CDS             complement(7094..9772)
                     /gene="mgtA"
                     /locus_tag="IW671_001980"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0898959.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10431..10868
                     /gene="ndk"
                     /locus_tag="IW671_001981"
     CDS             10431..10868
                     /gene="ndk"
                     /locus_tag="IW671_001981"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0898960.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10890..11756)
                     /locus_tag="IW671_001982"
     CDS             complement(10890..11756)
                     /locus_tag="IW671_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0898961.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11916..14009)
                     /locus_tag="IW671_001983"
     CDS             complement(11916..14009)
                     /locus_tag="IW671_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0898962.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4233 a         3126 c         2484 g         4735 t
ORIGIN      
        1 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
       61 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
      121 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
      181 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
      241 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
      301 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
      361 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
      421 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
      481 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
      541 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
      601 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
      661 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
      721 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
      781 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
      841 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
      901 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
      961 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
     1021 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
     1081 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
     1141 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
     1201 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
     1261 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
     1321 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
     1381 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
     1441 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
     1501 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
     1561 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
     1621 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
     1681 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
     1741 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
     1801 ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
     1861 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
     1921 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
     1981 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
     2041 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
     2101 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
     2161 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
     2221 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
     2281 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
     2341 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
     2401 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
     2461 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
     2521 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
     2581 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
     2641 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
     2701 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
     2761 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
     2821 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
     2881 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
     2941 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
     3001 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
     3061 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
     3121 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
     3181 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
     3241 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
     3301 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
     3361 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
     3421 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
     3481 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
     3541 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
     3601 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
     3661 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
     3721 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
     3781 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
     3841 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
     3901 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
     3961 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
     4021 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
     4081 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
     4141 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
     4201 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
     4261 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
     4321 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
     4381 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
     4441 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
     4501 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
     4561 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
     4621 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
     4681 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
     4741 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
     4801 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
     4861 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
     4921 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
     4981 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
     5041 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
     5101 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
     5161 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
     5221 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
     5281 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
     5341 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
     5401 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
     5461 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
     5521 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
     5581 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
     5641 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
     5701 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
     5761 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
     5821 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
     5881 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
     5941 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
     6001 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
     6061 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
     6121 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
     6181 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
     6241 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
     6301 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
     6361 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
     6421 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
     6481 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
     6541 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
     6601 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
     6661 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
     6721 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
     6781 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
     6841 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
     6901 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
     6961 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
     7021 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
     7081 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
     7141 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
     7201 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
     7261 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
     7321 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
     7381 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
     7441 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
     7501 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
     7561 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
     7621 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
     7681 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
     7741 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
     7801 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
     7861 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
     7921 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
     7981 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
     8041 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
     8101 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
     8161 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
     8221 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
     8281 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
     8341 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
     8401 aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat
     8461 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
     8521 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
     8581 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
     8641 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
     8701 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
     8761 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
     8821 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
     8881 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
     8941 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
     9001 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
     9061 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
     9121 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
     9181 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
     9241 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
     9301 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
     9361 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
     9421 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
     9481 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
     9541 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
     9601 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
     9661 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
     9721 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
     9781 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
     9841 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
     9901 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
     9961 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    10021 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    10081 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    10141 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    10201 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    10261 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    10321 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    10381 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    10441 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    10501 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    10561 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    10621 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    10681 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    10741 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    10801 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    10861 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    10921 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    10981 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    11041 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    11101 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    11161 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    11221 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    11281 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    11341 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    11401 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    11461 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    11521 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    11581 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    11641 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    11701 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    11761 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    11821 atacacgaaa aaaataaaaa aatgcttttt tcataaaaaa taccaacctt caagcattag
    11881 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    11941 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    12001 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    12061 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    12121 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    12181 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    12241 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    12301 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    12361 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    12421 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    12481 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    12541 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    12601 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    12661 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    12721 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    12781 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    12841 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    12901 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    12961 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    13021 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    13081 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    13141 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    13201 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    13261 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    13321 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    13381 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    13441 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    13501 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    13561 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    13621 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    13681 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    13741 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    13801 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    13861 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    13921 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    13981 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    14041 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    14101 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    14161 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    14221 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    14281 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    14341 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    14401 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    14461 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    14521 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctat
//
LOCUS       DACEKQ010000070        14223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEKQ010000070 DACEKQ010000000
VERSION     DACEKQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.074"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW671_001984"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW671_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0898963.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="IW671_001985"
     CDS             809..1243
                     /locus_tag="IW671_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0898964.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="IW671_001986"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="IW671_001986"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0898965.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="IW671_001987"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="IW671_001987"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0898966.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="IW671_001988"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="IW671_001988"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0898967.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="IW671_001989"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="IW671_001989"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0898968.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="IW671_001990"
     CDS             9514..10440
                     /locus_tag="IW671_001990"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0898969.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10622..11515
                     /locus_tag="IW671_001991"
     CDS             10622..11515
                     /locus_tag="IW671_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0898970.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11649..11774
                     /locus_tag="IW671_001992"
     CDS             11649..11774
                     /locus_tag="IW671_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0898971.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11956..13503)
                     /locus_tag="IW671_001993"
     CDS             complement(11956..13503)
                     /locus_tag="IW671_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0898972.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(13605..13958)
                     /gene="tnpB"
                     /locus_tag="IW671_001994"
     CDS             complement(13605..13958)
                     /gene="tnpB"
                     /locus_tag="IW671_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0898973.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(13948..14142)
                     /locus_tag="IW671_001995"
     CDS             complement(13948..14142)
                     /locus_tag="IW671_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898974.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         4877 a         2518 c         2929 g         3899 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa agagaacata
    10621 tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg atgatccgct
    10681 tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga ttctaagaga
    10741 attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat tgatcgatgc
    10801 aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat ttgatcctaa
    10861 agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat tgaagagact
    10921 ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt gtttcgaaga
    10981 tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag aattgctgag
    11041 gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag aacacttccc
    11101 gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt
    11161 caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg atcaaataga
    11221 agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta gtattttcca
    11281 tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat tagaattacc
    11341 tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt cattgacaat
    11401 ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga aatttatacc
    11461 gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa cctaagagag
    11521 cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct ttatggttaa
    11581 aagaattatt tgagtatttt gcctatctac cacctattag attcactaca tggtctggaa
    11641 acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta gtgataagaa
    11701 aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt ttatgggaag
    11761 aactagctga ttagattagg aagtccattt gataatccag aatcactaag aaacgaatgg
    11821 cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt gtttaaaacg
    11881 taagcgcccc atagaacagt acctgtacgt caaataaccc gacctataga atagaatcgg
    11941 gaatgtttgg cgtttttagt tacgagaaaa atgttgttgt acatccttgt tccaaggcaa
    12001 cagttgatct aaagtttctg ctgaaagtac gggaagattt ggtatcttat caaatagaaa
    12061 ctctagatac tttacaggat tcaatccgtt aagttctgct gttttatata cactcagaat
    12121 gattcctgaa gttcgtgctc cttcaagact tgtggaatgc aaccaatttt tccgtcctat
    12181 caccagctct ttgatatttc gctccgcacg attatttgtg agttctaaac gaccatcttc
    12241 aaaaataggt tcaaaaccac ttcgtagttt acacgcatat tgaacggctg catccaactt
    12301 ggattttgca acaggatcta tcgttgccat ccactcataa aaagcaataa ataatggacg
    12361 cagttcttgt tgccgacagc ttaatcgctt ctcaggagaa aagtctttcc atttcttttc
    12421 taggacaaac catttatcca attgtttgac tgcttgagct gcgggaatat ccgtgttttt
    12481 ctttcctttt ggaattgctt cgaaaaattt ccgccgaaca tgcgcgaaac aatagacaag
    12541 tcgtacactt tccacggcat tatacccgct atagccgtca cagtgtaaat accctgtaaa
    12601 ctccttgaga aaagttttcg gaacatctcc agcacgactt tcgtcgtggt gataataaac
    12661 gatgggatgc tgactgtctt ttcctgtaca aaactgccag taataggtat ctgttttggc
    12721 actttcaatc acacgataac ttgtttcatc cccgtgtaga atctcttctt gattaagata
    12781 gactcttaat cgttctccta aagggtctaa cgcgttttgc actgctttaa tatgccaatt
    12841 ggtgatatta tctctcgaaa tatccaaccc atgtgtttgt ttccaatagt tctcttgccg
    12901 ataggcagga actttctgac caaatttcat ccggatcgtt tccgccacta aactattgga
    12961 acctaagcta ttagaaatca aaggtttagg aacgatcgct tttttaaaag aagaaagtcc
    13021 ttctttttcg cagcttttac aacaataggt atgttgataa tgggtgagac aatccaacat
    13081 agcctgatgg aaacgaattt cttctcgtac ttttgtccgt ccgatatctt tcatctgtga
    13141 accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct ctgttatagg
    13201 taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt ttactggaat
    13261 aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa aaagattcaa
    13321 ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt tgttggttaa
    13381 ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa tggtttgttg
    13441 ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat ctgtagtttc
    13501 caaaattttt caccaccttg aatcgaactg attttgttac agaataccac aagaaacgca
    13561 caagaaatag gggttcaaca cattttttgt gttaaccccc cagcttaaaa aactcctttt
    13621 tttcctggca aaatagctcg aggttgatcg atacttaatc cttctaacaa ccatcgaacc
    13681 tgttggttcg ttaaggttcg aacttcgtct ttcgtccgag gccattttaa cttgccgttt
    13741 tctatccgct tataacagag aagaaaacca tcaccgtccc aatagagaag tttgaaacgg
    13801 tcttgacggt tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag ttgatactct
    13861 tgttgaataa gactggcaag accatcaatt ccttttcgca aatctgtttt tccgcacaca
    13921 atataaatat gtttggtttg aagtaaatca taaagcattg gacaacagtt ctttcaaaat
    13981 agcttgaaga atataattgt taataccgtt atagatcaaa acttcagaac cattctctct
    14041 tttgatgcgc atcgctaact tggcagccgc gggtttacct ttttctggtg cactcttttc
    14101 gtttcctaat tggacaggaa caatctcatt taatttatac atagaaaacc tccagacttc
    14161 ttgatagctc aagtatatct tgaggtgcat ttgttagtaa ggtactgttc tatggggcgc
    14221 tta
//
LOCUS       DACEKQ010000071        14054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEKQ010000071 DACEKQ010000000
VERSION     DACEKQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.075"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            62..763
                     /locus_tag="IW671_001996"
     CDS             62..763
                     /locus_tag="IW671_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0898975.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            760..1881
                     /locus_tag="IW671_001997"
     CDS             760..1881
                     /locus_tag="IW671_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0898976.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1896..3128
                     /gene="pepT"
                     /locus_tag="IW671_001998"
     CDS             1896..3128
                     /gene="pepT"
                     /locus_tag="IW671_001998"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0898977.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3260..4168
                     /locus_tag="IW671_001999"
     CDS             3260..4168
                     /locus_tag="IW671_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0898978.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4203..4475
                     /locus_tag="IW671_002000"
     CDS             4203..4475
                     /locus_tag="IW671_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0898979.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4486..5532
                     /locus_tag="IW671_002001"
     CDS             4486..5532
                     /locus_tag="IW671_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0898980.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5783..8392
                     /gene="clpB"
                     /locus_tag="IW671_002002"
     CDS             5783..8392
                     /gene="clpB"
                     /locus_tag="IW671_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0898981.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8543..9214)
                     /locus_tag="IW671_002003"
     CDS             complement(8543..9214)
                     /locus_tag="IW671_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898982.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9207..9701)
                     /locus_tag="IW671_002004"
     CDS             complement(9207..9701)
                     /locus_tag="IW671_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0898983.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9721..10941)
                     /locus_tag="IW671_002005"
     CDS             complement(9721..10941)
                     /locus_tag="IW671_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0898984.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11088..12923
                     /gene="lepA"
                     /locus_tag="IW671_002006"
     CDS             11088..12923
                     /gene="lepA"
                     /locus_tag="IW671_002006"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0898985.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13120..14031
                     /locus_tag="IW671_002007"
     CDS             13120..14031
                     /locus_tag="IW671_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0898986.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         4685 a         2489 c         2918 g         3962 t
ORIGIN      
        1 ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa ggagtgaagc
       61 tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg tccctgaatc
      121 cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat tgatattaag
      181 aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg aatcagcacg
      241 taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg caaatggtct
      301 agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta tgggaggttc
      361 actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg
      421 tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc aagaaaatca
      481 gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg aaatcattgt
      541 agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt tcgggccatt
      601 tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc ttcaacaaag
      661 aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat
      721 cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag cggacgcact
      781 ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg agacccagtc
      841 ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac attagacgtt
      901 cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat tgctaaacat
      961 cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga aaaaatgtat
     1021 gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat ggatattatt
     1081 tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga gagttattta
     1141 gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt ggatcatgca
     1201 cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga ttataccggt
     1261 acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc acaaccagcg
     1321 attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt tattttgccg
     1381 gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta tgaggaacct
     1441 gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg acgagtaggt
     1501 gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga agcgttccaa
     1561 ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg
     1621 atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc
     1681 tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc tgggttgatt
     1741 gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat cgagaaattt
     1801 gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga aacaaataca
     1861 aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt ttgccacgtt
     1921 ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg actccatcga
     1981 ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag ttaggcttag
     2041 ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca agcaatgttg
     2101 atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat tttaatgcag
     2161 taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc gtactagatg
     2221 cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat tatgaaggcc
     2281 acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa tcagggattg
     2341 cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct cacggtacca
     2401 tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg
     2461 agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt gagttgcagt
     2521 atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat gtacatccag
     2581 ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt cataatcagc
     2641 ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc catttgatgg
     2701 cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat catgatcgtg
     2761 aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg aatcagcgat
     2821 ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg aaagaaatca
     2881 ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag cttggtatct
     2941 ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct tacatgggta
     3001 ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc gaatttgttt
     3061 ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa
     3121 agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg atggaatgtc
     3181 atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc ttttttgaag
     3241 agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa gttcagggaa
     3301 agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa agcagccaga
     3361 attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat tagtcaagaa
     3421 agcagaagcc atagaagctg atactttagt aattataggt ggagacggca cgattcatca
     3481 tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac ttccaggtgg
     3541 aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag aagctgctga
     3601 cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg atgaagtcat
     3661 tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca gtcaagaaga
     3721 aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat tgctgaagaa
     3781 gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa aatcccaact
     3841 gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg actcagcgac
     3901 cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt tccgatttat
     3961 tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca ttacttattt
     4021 cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc gaacggatgg
     4081 agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat taacagttta
     4141 ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt ggaagaagag
     4201 taatggatgt attgttacac aatcttttgt ggttttacct tttcattgtg aatttttact
     4261 tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg cgtgttcctg
     4321 agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg atctctcgaa
     4381 aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct gcaggtgtac
     4441 ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc gtctaaagga
     4501 acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc cattatttat
     4561 tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac cagtgttgtt
     4621 gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg gcgctcaatc
     4681 aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt cttgacctct
     4741 agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat ctcggagatt
     4801 tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac agcactccat
     4861 atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt catccaattc
     4921 tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg tatccttttg
     4981 acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt tcaaatattt
     5041 tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg ggtggataca
     5101 tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca agacaggact
     5161 gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta tgtacttcct
     5221 tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat ttcgtttatc
     5281 agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg ttcctttgaa
     5341 tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt ttcttcgctt
     5401 ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt gtatgtgatt
     5461 gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga gaataaagaa
     5521 aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga aaaacgttat
     5581 ggataagata attttacgtt tgtttggctc tttttttata tacatacaat caattttgtt
     5641 tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ccatgtgtat attttgacca
     5701 aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt gatcttacct
     5761 ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc ttcaagaagc
     5821 gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg acattgccca
     5881 tgtatggaaa atctttttac aacctaatca ttttggtcga aatttctata cggatgccgg
     5941 attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt atccagttgt
     6001 ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc gattattgaa
     6061 tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg aagtcgttat
     6121 tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata aaaacggatt
     6181 atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa gagtgacctc
     6241 acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt tagtacagca
     6301 agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc gtgatgtcat
     6361 ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac ccggtgtagg
     6421 gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg tgcctgaaaa
     6481 tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg gagcaaaatt
     6541 ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa aaagtgatgg
     6601 acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa aaacagaagg
     6661 aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat tacatttgat
     6721 cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag cgttggaacg
     6781 tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca gtattctgcg
     6841 tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca acgcattagt
     6901 agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg acaaagcaat
     6961 cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta tgccaacgga
     7021 attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg cactgaaaaa
     7081 agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat tagctgaact
     7141 tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag aagtcaattc
     7201 tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag atgcagaaaa
     7261 taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc aattagaaaa
     7321 agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg ttcaagagtc
     7381 cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc ccgtaacgaa
     7441 acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc ataaacgagt
     7501 gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt cacgggctgg
     7561 attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa ctggtgtcgg
     7621 aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag atcatatggt
     7681 acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag tcggagctcc
     7741 tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca gacgaaatcc
     7801 ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct tcaatatctt
     7861 gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag tagatttcaa
     7921 aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg aaggcgtgac
     7981 tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat tacgtggaaa
     8041 cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac cattgagcct
     8101 agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac gtctggaaca
     8161 tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg aaaatgctta
     8221 tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag tagaaacacc
     8281 actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa cgattacttt
     8341 acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct aatcttccat
     8401 tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc ttttttcaaa
     8461 taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt gaagctgccc
     8521 gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta taactttcaa
     8581 agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag gcagcaccta
     8641 tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg acaaacagtg
     8701 tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg cataagaatt
     8761 cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat aagaggaaca
     8821 accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc aatccatagc
     8881 caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa aaagagatga
     8941 tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa aaccagtaaa
     9001 acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact tgtggcatga
     9061 aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga gaagtcgaat
     9121 atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca aaagcaagta
     9181 aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc taaaaatctt
     9241 tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa tttgatcaaa
     9301 cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg tagtgcagcg
     9361 atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc gatcactggg
     9421 atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat cgtcagccca
     9481 tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc aagagcaatc
     9541 attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg aggaagatag
     9601 atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt atataacaaa
     9661 tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc atccttttta
     9721 tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta gtgacttcag
     9781 tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac ggcgataatt
     9841 catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta taggttcatt
     9901 taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat tattccaagg
     9961 agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa acggaaaccg
    10021 ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga tctgctcttt
    10081 gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat ctccatgtac
    10141 ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc gagcttcttg
    10201 atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt agaacagaac
    10261 acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat cttcattttc
    10321 gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat ggttttgttg
    10381 ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc gctcaaccat
    10441 accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg aaagtttatc
    10501 agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat acaaataaga
    10561 gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat cttgcatcaa
    10621 tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca ttttataacg
    10681 tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca gtttattttc
    10741 gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac ggataaaagc
    10801 tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga acacatagga
    10861 aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt caagattttc
    10921 tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa cagtatagca
    10981 aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa ttactgcttt
    11041 tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg gatataaaga
    11101 aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat atcgaccatg
    11161 ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg tctcgcgaaa
    11221 tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc acgattaaat
    11281 taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc tttcacttga
    11341 ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt gctgcctgtg
    11401 agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca ttggccaatg
    11461 tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa atcgacctac
    11521 ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga atcgatgcta
    11581 gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt ctcgagcaga
    11641 tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa gctttgatct
    11701 ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc acggacggta
    11761 tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt gatgttacag
    11821 aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc ggtgatgtcg
    11881 gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat acagtaacgt
    11941 tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat ccgatggttt
    12001 attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa gctttagaaa
    12061 aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa gcattaggtt
    12121 ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa gaacgcttgg
    12181 aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac catgttaata
    12241 aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa cctgtaacga
    12301 ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat gattttgtcg
    12361 gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg gattatttag
    12421 atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc tttgactttt
    12481 tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa atgtctggtt
    12541 atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa gtcgatgcat
    12601 taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc gttgaaaaac
    12661 tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct attggacaaa
    12721 aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct aaatgttatg
    12781 gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg aagaagcgga
    12841 tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt ctgaaaatgg
    12901 atgagcaaga tagtgtgaaa taacttataa tcaatcgaaa aacctattaa atcaaccttt
    12961 ttgagtagat gcaacactga ggattatctg tataaaagat tttaaactgt catttggtta
    13021 tcacggcgat actaaaatta acaaaattgg aatatttttt cttgaagatt tttagctgtc
    13081 agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa ttcaagtgga
    13141 agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt agctgattta
    13201 gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa aatccctaaa
    13261 gacgtagata atactctaag caagattttg aatatagaaa ctctaataaa agatgaggaa
    13321 gataagattg agattctcat cggtcaacta attgcataca gagatatgaa taagttactt
    13381 aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat
    13441 agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat
    13501 gaaaaagaaa tttcgaagga gagtaatcgt gatagtgaga tatttagttt atcaaaagac
    13561 gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt
    13621 gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa
    13681 aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc
    13741 actgttcctt accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga
    13801 gttagcgatt ttaacaagat aaattctttt agtatgggat taaggatgag gaaaattagg
    13861 aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa
    13921 tggtccatag ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa
    13981 tatgcttata taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt
    14041 acgtcgatac ttat
//
LOCUS       DACEKQ010000072        14025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEKQ010000072 DACEKQ010000000
VERSION     DACEKQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.076"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..460
                     /locus_tag="IW671_002008"
     CDS             140..460
                     /locus_tag="IW671_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898987.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IW671_002009"
     CDS             912..1112
                     /locus_tag="IW671_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0898988.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IW671_002010"
     CDS             1259..4048
                     /locus_tag="IW671_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0898989.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IW671_002011"
     CDS             4095..4622
                     /locus_tag="IW671_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0898990.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IW671_002012"
     CDS             4643..5833
                     /locus_tag="IW671_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0898991.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IW671_002013"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IW671_002013"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0898992.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IW671_002014"
     CDS             8224..8898
                     /locus_tag="IW671_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0898993.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IW671_002015"
     CDS             8895..9236
                     /locus_tag="IW671_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0898994.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IW671_002016"
     CDS             9266..9625
                     /locus_tag="IW671_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0898995.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IW671_002017"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IW671_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IW671_002018"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IW671_002018"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0898996.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IW671_002019"
     CDS             12541..14007
                     /locus_tag="IW671_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0898997.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4648 a         2429 c         2997 g         3951 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggtgaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttac
//
LOCUS       DACEKQ010000073        14021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEKQ010000073 DACEKQ010000000
VERSION     DACEKQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.077"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..799)
                     /locus_tag="IW671_002020"
     CDS             complement(<1..799)
                     /locus_tag="IW671_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0898998.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRT"
     gene            1072..3543
                     /locus_tag="IW671_002021"
     CDS             1072..3543
                     /locus_tag="IW671_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0898999.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3646..5382)
                     /locus_tag="IW671_002022"
     CDS             complement(3646..5382)
                     /locus_tag="IW671_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0899000.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5379..6083)
                     /locus_tag="IW671_002023"
     CDS             complement(5379..6083)
                     /locus_tag="IW671_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899001.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6214..6717
                     /locus_tag="IW671_002024"
     CDS             6214..6717
                     /locus_tag="IW671_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0899002.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6677..7015
                     /locus_tag="IW671_002025"
     CDS             6677..7015
                     /locus_tag="IW671_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0899003.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7036..8454
                     /gene="ffh"
                     /locus_tag="IW671_002026"
     CDS             7036..8454
                     /gene="ffh"
                     /locus_tag="IW671_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0899004.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8566..9144
                     /locus_tag="IW671_002027"
     CDS             8566..9144
                     /locus_tag="IW671_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0899005.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9148..9669
                     /locus_tag="IW671_002028"
     CDS             9148..9669
                     /locus_tag="IW671_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0899006.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9748..10302)
                     /locus_tag="IW671_002029"
     CDS             complement(9748..10302)
                     /locus_tag="IW671_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0899007.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10555..10830
                     /gene="rpsP"
                     /locus_tag="IW671_002030"
     CDS             10555..10830
                     /gene="rpsP"
                     /locus_tag="IW671_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0899008.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10842..11093
                     /locus_tag="IW671_002031"
     CDS             10842..11093
                     /locus_tag="IW671_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0899009.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11218..12009
                     /locus_tag="IW671_002032"
     CDS             11218..12009
                     /locus_tag="IW671_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899010.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12179..12700
                     /gene="rimM"
                     /locus_tag="IW671_002033"
     CDS             12179..12700
                     /gene="rimM"
                     /locus_tag="IW671_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0899011.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12690..13451
                     /gene="trmD"
                     /locus_tag="IW671_002034"
     CDS             12690..13451
                     /gene="trmD"
                     /locus_tag="IW671_002034"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0899012.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13587..13934
                     /gene="rplS"
                     /locus_tag="IW671_002035"
     CDS             13587..13934
                     /gene="rplS"
                     /locus_tag="IW671_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0899013.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4603 a         2579 c         2896 g         3943 t
ORIGIN      
        1 aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga ttgatgtgtt
       61 ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt ttaagcaact
      121 gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg gcctttctag
      181 gaaagcgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg atcgtaaata
      241 gctgattggt tgtcccatca acacaaataa aactcatgga accagaaaca gaattcattg
      301 atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtggc tggtaatgct
      361 tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac ttcttagcga
      421 ttagtttacg tgattgattt tcgtacagtt ccatgagaat cgcctgcttc agttcctttg
      481 ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt ccacaatcat
      541 ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg actttcaaca
      601 tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga cagtgttcac
      661 aataggaaga agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt
      721 ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt
      781 ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa
      841 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
      901 gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag
      961 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
     1021 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
     1081 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
     1141 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
     1201 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
     1261 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
     1321 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
     1381 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
     1441 aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata
     1501 gatgacattg ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa
     1561 gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
     1621 ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
     1681 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
     1741 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     1801 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     1861 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     1921 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     1981 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     2041 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa
     2101 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     2161 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     2221 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     2281 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     2341 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     2401 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     2461 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     2521 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     2581 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     2641 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     2701 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     2761 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     2821 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     2881 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     2941 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     3001 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     3061 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     3121 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     3181 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     3241 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     3301 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     3361 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     3421 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     3481 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     3541 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     3601 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     3661 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     3721 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     3781 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     3841 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     3901 aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt tgataattgg
     3961 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     4021 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     4081 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     4141 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     4201 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     4261 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     4321 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     4381 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     4441 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     4501 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     4561 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     4621 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     4681 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     4741 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     4801 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     4861 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac
     4921 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     4981 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     5041 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     5101 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     5161 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     5221 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     5281 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     5341 aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac
     5401 ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt
     5461 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     5521 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     5581 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     5641 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     5701 tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact
     5761 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     5821 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     5881 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     5941 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     6001 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
     6061 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
     6121 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
     6181 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
     6241 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
     6301 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
     6361 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
     6421 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
     6481 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
     6541 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
     6601 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
     6661 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
     6721 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
     6781 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
     6841 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
     6901 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
     6961 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
     7021 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
     7081 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
     7141 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
     7201 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
     7261 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
     7321 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
     7381 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
     7441 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
     7501 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
     7561 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
     7621 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
     7681 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
     7741 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
     7801 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
     7861 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
     7921 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
     7981 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
     8041 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
     8101 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
     8161 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
     8221 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
     8281 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
     8341 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
     8401 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
     8461 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
     8521 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
     8581 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
     8641 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
     8701 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
     8761 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
     8821 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
     8881 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
     8941 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
     9001 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
     9061 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
     9121 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
     9181 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
     9241 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
     9301 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
     9361 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
     9421 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
     9481 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
     9541 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
     9601 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
     9661 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
     9721 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
     9781 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
     9841 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
     9901 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
     9961 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
    10021 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
    10081 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
    10141 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
    10201 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
    10261 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
    10321 aactatctat aataatctta caaaagataa gtaactggag caaataaaaa cacttactga
    10381 caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct
    10441 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
    10501 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
    10561 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
    10621 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
    10681 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
    10741 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
    10801 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
    10861 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
    10921 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
    10981 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
    11041 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
    11101 tgctgtactc gaatattcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
    11161 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    11221 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    11281 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    11341 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
    11401 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    11461 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    11521 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    11581 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    11641 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    11701 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    11761 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    11821 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    11881 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    11941 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    12001 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    12061 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    12121 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    12181 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    12241 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
    12301 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    12361 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    12421 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    12481 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    12541 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    12601 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    12661 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    12721 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    12781 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    12841 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    12901 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    12961 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    13021 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    13081 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    13141 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    13201 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    13261 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    13321 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    13381 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    13441 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    13501 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
    13561 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    13621 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    13681 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    13741 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    13801 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    13861 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
    13921 aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct
    13981 tttttcttag catgtaattt gaacaccctg atccagtttt t
//
LOCUS       DACEKQ010000074        13951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEKQ010000074 DACEKQ010000000
VERSION     DACEKQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.078"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            234..476
                     /locus_tag="IW671_002036"
     CDS             234..476
                     /locus_tag="IW671_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0899014.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            473..943
                     /locus_tag="IW671_002037"
     CDS             473..943
                     /locus_tag="IW671_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0899015.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1071..2285
                     /locus_tag="IW671_002038"
     CDS             1071..2285
                     /locus_tag="IW671_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0899016.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2995..3183)
                     /locus_tag="IW671_002039"
     CDS             complement(2995..3183)
                     /locus_tag="IW671_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899017.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3274..3483
                     /locus_tag="IW671_002040"
     CDS             3274..3483
                     /locus_tag="IW671_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0899018.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3595..5208
                     /locus_tag="IW671_002041"
     CDS             3595..5208
                     /locus_tag="IW671_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0899019.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5243..6454
                     /locus_tag="IW671_002042"
                     /pseudo
     CDS             5243..6454
                     /locus_tag="IW671_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6477..7403
                     /gene="glsA"
                     /locus_tag="IW671_002043"
     CDS             6477..7403
                     /gene="glsA"
                     /locus_tag="IW671_002043"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0899020.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7611..9071
                     /locus_tag="IW671_002044"
     CDS             7611..9071
                     /locus_tag="IW671_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899021.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9292..11055
                     /locus_tag="IW671_002045"
     CDS             9292..11055
                     /locus_tag="IW671_002045"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0899022.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11139..11597
                     /locus_tag="IW671_002046"
     CDS             11139..11597
                     /locus_tag="IW671_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0899023.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11706..12389
                     /locus_tag="IW671_002047"
     CDS             11706..12389
                     /locus_tag="IW671_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0899024.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12536..13150
                     /locus_tag="IW671_002048"
     CDS             12536..13150
                     /locus_tag="IW671_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0899025.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13166..13372
                     /locus_tag="IW671_002049"
     CDS             13166..13372
                     /locus_tag="IW671_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899026.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13883..13951
                     /locus_tag="IW671_002050"
                     /pseudo
     CDS             13883..13951
                     /locus_tag="IW671_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
BASE COUNT         4836 a         2200 c         2907 g         4008 t
ORIGIN      
        1 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
       61 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      121 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      181 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      241 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      301 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      361 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      421 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      481 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      541 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      601 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      661 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      721 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
      781 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
      841 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
      901 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
      961 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1021 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1081 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1141 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1201 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1261 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1321 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1381 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1441 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1501 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1561 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1621 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1681 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1741 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     1801 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     1861 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     1921 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     1981 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2041 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2101 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2161 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2221 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2281 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2341 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2401 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2461 gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg acggaaattc
     2521 gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg tattcaaaca
     2581 ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc aacttaacaa
     2641 tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt gttggcagta
     2701 acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc atcatcaact
     2761 tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa atttttccat
     2821 ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag aaaatcaacc
     2881 gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt ttggattttg
     2941 gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt tctttcaatc
     3001 gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag ccgttgaaac
     3061 aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg ttcttgtaaa
     3121 agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact cccgtacagc
     3181 cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag aactatgcag
     3241 aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
     3301 aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt
     3361 ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc cgaaaagggc
     3421 aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca gacaatcgca
     3481 taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt
     3541 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
     3601 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
     3661 gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa agaacatcat
     3721 ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
     3781 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
     3841 gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga attgtatttc
     3901 cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga aaaaggcgaa
     3961 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
     4021 aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg tgcttatgct
     4081 ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga tgatgaaaca
     4141 aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag tgcaaaaatc
     4201 acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt tacaagatac
     4261 ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga gtggacgatt
     4321 gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt tcacaatatg
     4381 cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag tgaatggttt
     4441 cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg tgtgcaggag
     4501 cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt tgcagggctt
     4561 gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac aaataaaacg
     4621 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa ttgttctatg
     4681 cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca gtattgggct
     4741 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     4801 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     4861 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     4921 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     4981 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5041 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5101 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5161 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5221 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5281 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5341 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5401 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5461 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5521 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5581 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5641 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5701 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     5761 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     5821 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     5881 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     5941 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6001 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6061 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6121 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6181 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6241 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6301 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6361 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6421 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6481 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6541 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6601 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6661 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6721 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     6781 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     6841 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     6901 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     6961 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7021 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7081 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7141 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7201 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7261 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7321 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7381 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7441 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7501 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7561 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7621 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7681 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7741 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     7801 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     7861 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     7921 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     7981 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8041 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8101 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8161 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8221 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8281 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8341 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8401 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8461 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8521 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8581 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8641 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8701 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     8761 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     8821 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     8881 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     8941 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9001 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9061 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9121 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9181 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9241 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9301 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9361 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9421 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9481 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9541 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9601 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9661 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9721 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     9781 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     9841 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     9901 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     9961 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10021 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10081 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10141 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10201 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10261 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10321 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10381 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10441 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10501 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10561 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
    10621 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10681 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10741 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    10801 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    10861 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    10921 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    10981 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11041 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11101 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11161 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11221 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11281 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11341 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11401 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11461 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11521 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11581 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11641 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11701 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    11761 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    11821 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    11881 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    11941 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12001 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12061 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12121 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12181 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12241 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12301 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12361 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12421 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12481 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12541 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12601 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12661 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12721 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    12781 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    12841 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    12901 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    12961 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13021 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13081 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13141 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13201 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13261 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13321 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
    13381 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
    13441 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
    13501 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    13561 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    13621 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    13681 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    13741 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    13801 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    13861 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ttttgggtcc
    13921 ttatttgact ttgtccatga tcggtctttt a
//
LOCUS       DACEKQ010000075        13932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEKQ010000075 DACEKQ010000000
VERSION     DACEKQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.079"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..720)
                     /locus_tag="IW671_002051"
     CDS             complement(64..720)
                     /locus_tag="IW671_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0899027.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            913..1191
                     /locus_tag="IW671_002052"
     CDS             913..1191
                     /locus_tag="IW671_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899028.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1210..2418
                     /locus_tag="IW671_002053"
     CDS             1210..2418
                     /locus_tag="IW671_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0899029.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2566..2931)
                     /locus_tag="IW671_002054"
     CDS             complement(2566..2931)
                     /locus_tag="IW671_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0899030.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3078..3851)
                     /locus_tag="IW671_002055"
     CDS             complement(3078..3851)
                     /locus_tag="IW671_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0899031.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4025..4885)
                     /locus_tag="IW671_002056"
     CDS             complement(4025..4885)
                     /locus_tag="IW671_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0899032.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4885..6303)
                     /locus_tag="IW671_002057"
     CDS             complement(4885..6303)
                     /locus_tag="IW671_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0899033.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6360..6809)
                     /locus_tag="IW671_002058"
     CDS             complement(6360..6809)
                     /locus_tag="IW671_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0899034.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7254..7517)
                     /locus_tag="IW671_002059"
     CDS             complement(7254..7517)
                     /locus_tag="IW671_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0899035.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7616..8911)
                     /locus_tag="IW671_002060"
     CDS             complement(7616..8911)
                     /locus_tag="IW671_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0899036.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8908..10179)
                     /locus_tag="IW671_002061"
     CDS             complement(8908..10179)
                     /locus_tag="IW671_002061"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0899037.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10344..11213)
                     /locus_tag="IW671_002062"
     CDS             complement(10344..11213)
                     /locus_tag="IW671_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0899038.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11505..13115)
                     /locus_tag="IW671_002063"
     CDS             complement(11505..13115)
                     /locus_tag="IW671_002063"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0899039.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3961 a         2935 c         2375 g         4661 t
ORIGIN      
        1 tatttttccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctttgt
       61 tcatcaaaag ataatattgt cttctaacaa gtccatattc aaaataacga tatgatggtc
      121 tttaacggtg ataataccat cttttttcag ttgttttaaa attctgttta cactgctagc
      181 agttgagatg ccacaaaatt tggcgatttc ttcattcgtg atcgaaaaat cgatcagcat
      241 cccttcttct gtccgcacac cgaataaatc atataattcg taaagttgcg tacaaacagc
      301 tcccagcttt ccgtttaata gcatttgctg catttttttc atcgaataaa gcagacgata
      361 gcggtaatac tcttttacat agtgttgtag ctttgggtct tggttgatat tttgccaaaa
      421 gcgcacacga ttgattcgat aaaactctgc atattctgat tcaactcgaa tattatacgg
      481 cgaatcgatg aattgtgaat attcgtcttt taaaagagag acgatttcaa gatctttgat
      541 atagcgaagg ttaaattctc tcccatcatt tgaaataaca cttgctttta ccgttccctc
      601 tttcaagata tacgtatggg aatcttccat cccttcgaat gtcaaatatt tatgatatcc
      661 tttgcgtata atcggaaaat catttttatc caaatattcc tttaagactc tactatccaa
      721 tgatgctccc ccatccaaac tttcatctaa aaagatcata cagtacttta aaacttttta
      781 aagtccaaag gtattttact aatgatatta acatttgtta agtacctttt tccatcactt
      841 atgcgatttt tttccttttt tttattcgtt atactgcaga agtaaaattt tttaagaata
      901 gagggaaaaa acatgaatta ctggatgaac acaatcatta atcgattaga aacagcatat
      961 caaacaagat ttgatatgaa agcttcttta gtctttttaa atgatgcgta tcaaaactcg
     1021 atcgaattga tcaaagcagt ggatgagaat ccaacaaacg aatgcgaaga atttttaaat
     1081 ctgtttatgt ctacaagaga tttatttatt cgccaacttg ttgatcgcta tccatccaat
     1141 tatcacgatg tagaagttca aatacaaaaa ttaaaagcat attcagctta aggggaatgt
     1201 ataacataca tggaaaagaa attatttaat aaatatttta ttggaatcac attgatcaat
     1261 tttattgtct acctgattta ttatctgttg atggtcatca tggcagtcat tgcgcaagac
     1321 accttacacg ccagcttgag ccaagcaggt tttgcttctg ggatctatat catcggaaca
     1381 ttgctggcac gcttattcat ggggaaacag ttagaactat ttggtcggaa gtttacctta
     1441 agaggcggcg cgatcttcta tctcctttca acattagcat atctttatac gccaactatc
     1501 ggggcattgt atttagttcg attcttgaat ggattcggct acggtacagt ttctacagct
     1561 acaaatgcta tcgttactgc atatatccct gcatctaaaa aaggggaagg catcaattat
     1621 tatggattaa gtactagttt agctgccgct gttgggccat ttttaggaat gattttgctg
     1681 aatatgactg attttcgttt cattatctgg ttttcaattg tactggtctt ctttgttacg
     1741 attgcctgcc ttgtctttcc tgtaaaaaat attccattat ctgaagaaca aaaaatatct
     1801 cttcgctcat ggaatatcaa tagttttatt gaaaagaaag cacttttcat tacaggaatt
     1861 gcctttttga tgggtctttc ttattcaagt gttttatcat ttttatcttc ttatacaaaa
     1921 gtaatccact tagttgctgc tgggtcattt ttcttcgtcg tatatgcttt ggtcatcaca
     1981 tttacacgtc cattgactgg aaggattttc gatgtcaaag gagaacaata cgtgatgtat
     2041 cctagctatt tattcttaac agcaggatta tttttactga gtgtaacgac gaatagtgtg
     2101 acattgctta tctctggagc attagtcggc ctaggttatg gtacgtttat gtcaaatgga
     2161 caggctgttt gtttaaaaat cgtccgcgaa catcgaatca gtattgcttt atctacctac
     2221 tttattggtt tagacctagg cttaggcgtt ggtccttatt tgatggggat gctaaaatct
     2281 cttacttcat ttagaggatt gtatgtgatt gccggagtca ttccattgat atgtacagca
     2341 ctctatcttg ttaatggccg aaaacatagt catgaagcag agggcgaatt gattgaatca
     2401 ttgaaaaatg aaatttgatc ttgatgaata gataaaaaaa accgttcaga tctgaaaata
     2461 atctgagcgg ttttttgagt aaaaatagtt tatgattatt cttaattaaa catctattta
     2521 tttttaaagc tttcatttat cgataagaat agtttaactg ttgatttatt ccattttccg
     2581 ttcatctggt acgatgcctt gacttagcaa ccacttgtgg aagaaccatt ctgccacacc
     2641 gatcaataga gcaggaatca atactttcat ccaaatatcg actgtatcat tcataaaata
     2701 tagatagagc cacaagataa ctgcagctga accagcatct gcgatcgtcg ctgcgatgtt
     2761 ccctacttta cgtaagatca acagatcccc tagcacatag atcatgacta caataattac
     2821 cccaacaatc gttgaatcca taaagctaac accgtataaa cctgtcagca cgacccacag
     2881 aatcgcccag atcatgattg ccttcactac caacgccttc acatgtttca ttttctgcac
     2941 tcctttctcc tggataaaaa agtaagatta tctaacaaga taaccttttc attattatac
     3001 catcggctga gagaagcgag caaagataag gtcctatcta aaaatgagga agccacttag
     3061 aacattcacg taataattta tccgtacaga cattcgatat ggtgttcctt tactttcgca
     3121 tactgttctt ttgtcagttc tttacttgtc accagataat cgatttcatg aaaagcaatg
     3181 gcttgatgca tgaatttatg tgcaattttt tgctgtgtaa ctaaaacggc aagccggtct
     3241 gattgttttc taaaagcttt tttgaccatt gcctctcctt catgagtttc tgttactcct
     3301 tggctgaaat ctagcccgcg acaactcatt atcgctaaat ccgcataata tgacaatgca
     3361 tcgttatagg cttttgaacc gttgattgtt gctcgtttcg gaacaattgc ccctcccaat
     3421 acagatactt ttgccgttgt atattcactc aacatagcag ctgtgacgat gccattcgta
     3481 atgatctgca aaccatcaaa tcttgctagg tttctgacta agtgtaaaga ggttgtactg
     3541 gaatccaaga ataagaccat atcatctcga atcaaagaag aagctgtatc tgcaatctta
     3601 cgtttttctg tttcttcttc taatttggtg atcttcatca ggtaaggcac gtcaattttt
     3661 cgattgtcaa taagcatggc gccaccgcga actctacgga tcaatccact tttttccaga
     3721 aaaacaaggt cacgtttaat cgtggcttcg ctataattta ctaactcgat caaatcggtg
     3781 actcttgcaa attggttgtt ttttagataa ttcacaatct cctgctgtct attctccact
     3841 tcattcaaca taaaatcacc ccatttactt tatatagtaa caggaaaaaa gaggttgtga
     3901 cagaagtgat tggcttcgag aaataagaag aaatttttcg aaaaaactac ttctgaacat
     3961 cgtgtgtggt taagagggta tgacaagact attgtcatac cctcttgacc acacattttt
     4021 atttttagta taaactagcc ttgcctactg tgttcaaaat ttccatttta tgtttgacca
     4081 cttcttgtac ctttttatta gctgctggat aaattgcatt cggctcgtac atcgatggat
     4141 tttcactcaa tatttctctc aaagcattga aaaagacgct tttaagatct gagctcaaat
     4201 tcactttggc aactccgtat tttactgctt cgctgacttc tgcgtccgga ttacctgaac
     4261 cgccatgtaa aacaaatgga agatcgattt gttggttcaa ttctttcagt aagtcgatat
     4321 ttaattttgg agttctgttt ttcggataca agccatgcgc tgttccaatt gctacagcaa
     4381 gtgtatcgat tcctgtttct ttcacaaaaa tcttcgcttg atcaggatct gtataaatga
     4441 ttgtttcagc acctccttca gcagaaccat tattcccaat cgttccaagc tcagcctcca
     4501 ctgagacatt taccgcatga gctaacgcaa ccactttttt cgtaatctgt acattgtcgt
     4561 cgaatgattt caaagacgca tcgatcatca cggaagaata gccgtttcga atcgcccgta
     4621 agacatcttc tatcgtaccg ccatgatcca agtggataac gatcggcagt ttacttgtat
     4681 aagcataggc tcttactgcc gcaacaaact catcacttaa ataggcgatt tcatcaggat
     4741 gaatttctaa gattactggt gcttctaatc tctctgcttc ttcgatgatt gctttcatca
     4801 tatcatagct gcaaatattg aaggctggta ctgcaaattg attctcttta gccactttca
     4861 ataactcttt cattgtatat aacattacgc ttccacctca tttgttagat tttcattttt
     4921 cgtccacttg tcttgttttt tctctttcac tgtttttttc aatagtccaa gcataaaggc
     4981 tgtagtgact gtacctgatg caattgccaa gacatacata ggtaaattgt tcataacatt
     5041 tggaattagc atcacccaaa tccctccatg aggtgccatt gaagtaactc ctactgccat
     5101 ggaaatccct gcagcgactg ctgaacctgt catcaaacta ggtatcacgc ggatcgggtc
     5161 agataccgca aacggtatag caccttctgt aatatacgaa gcgcctaaaa tccagcatga
     5221 ttgacctgaa gtccgttctt ctttggtaaa tttatgtttg aataataaag tggctaaagc
     5281 aatacctagg ggaggtgtca ttcctgcagc catacaagca gcaatcggtg catttactcc
     5341 agctgacatc aaaccgatcg agaaagtcga agtcgattta ttgatcggcc cacccatatc
     5401 agctgccatc atgatcccga taagtaatcc gaacaatagc ccggatgtat ttcccaagct
     5461 attcagccaa tttgtcaaag catccaacac ccatgtgatc ggttgttcaa tcacaaatac
     5521 catcaaaagc ccaataatta agatcgataa aaaggggacg acaatcagct gataaatagc
     5581 agaaaattct ttttttacgc taattttatt tgccagaaaa tcacagatat accctgctag
     5641 cacgccacca atcattccgc caagaaaacc tgaaccacct gtagaggcca gtaatccggc
     5701 aacaattcct ggggcaaaac ctgcttttcc agcaatcgat gtagcaatcc ccgcgcctaa
     5761 cgcaggaacc atcatggcaa acgctgtatc tcctccgatc cttgaaaatg cagctgctaa
     5821 cacattatag tctgctgagt tgggatcagc cgctgtaatc ccaaaggcaa aactcagtgc
     5881 gatgatgatt ccgcctgcga tcacaaatgg aagcatgtaa ttcacaccat tcatgaaatg
     5941 attatagatt gtcggctttt tctcatttgc agctggttct ttttcattag acacaacgaa
     6001 tacgccaata tctctcaatg cttcgtctat tactacttgc ggttctttga ttgctcggct
     6061 tgtagagaca cgctttactt ttttcccggc aaatcggtcc aattcgattt ctttatctac
     6121 tgcaagaatg attgcatctg cctctttgat ttcttgttca gataactgat tttctgtttt
     6181 tacaccttgt gtttcgaatt ttgcttgatg gtttagattt cttgctgtta cttctaattt
     6241 ttctgccgac atataagtat gagcaattcc agtcgggcaa gcagttacac caacgatttt
     6301 catgattatt ttcctccttt ttcttcacta tcgatactcg gacaaagact ctactatttt
     6361 cattcttgac taatctgtct gatgatatct gctggcgtag tggcttcttt tattcccttt
     6421 ctaaaatctg cattcatcaa tcgtctggag agtgtgctga gaatctctag atgcatatct
     6481 gctgattttt caggaactac gatggcaaaa atatattcaa ttggcatttc atctatcccg
     6541 ctatagtccg ttaatttggc tgtatgaaaa aaacacagtg ctggttttgt cacatatttg
     6601 ctttttgcat ggggaatccc aaatccatct tctattcctg tggagatctc ctcctctcgt
     6661 ttgaatagtg ccttaacgac tttttgaaca tttcttacct tatttgtttt tttcaacaca
     6721 tcccctattc gctcaaataa tgatcctttt gaatctatcg atggatcgaa aacaatcagt
     6781 tcttctgcga tcaattgatc aaacctcata aaaaacctct cccccacaaa aaatgttaac
     6841 gttttcttgg aactgaggtc agtatagcat cgctagctgt accgttcaat attatgaatg
     6901 aacagaggaa aaagacccat catccgcgtg tataagtcat taaaatgaac gatctagctc
     6961 acaaacaatc gcaagtctat aacaaaacaa aaagccttcc ttgaaaggaa agcttagctt
     7021 ctaattttag gtaaatggtt ttttacttcg aaataacccc tcttcatttc gttaatataa
     7081 ggtgttttta tcaagtggat aattaaaact atatactagt ttttcattgt tttgtgcatt
     7141 atgatcctac gactttttca gcatcaaacg aagttttaaa ttggcaaaaa acataaaaaa
     7201 acagactatc cctatcagat agtctgctaa acgttgagtt atcagcgttt ttattattcc
     7261 gcagcgtttg cgtctttgag accgtatttt ttgttgaaac ggtccacacg tccgtctgct
     7321 tgtgtgaatt tttgacgtcc agtgtagaat ggatgtgagt cagaagttac ttcgacacgg
     7381 atgactgggt atgtattacc gtcttcccat tcaactgttt cttgtgaagt ttttgttgaa
     7441 cctgacaaga atttgaaacc agttgttgaa tccatgaata ctactgggtg gtaatctgga
     7501 tggatgtttt ctttcatgat tttttcgctc ctttgccctg ctccatttgc tgaaccagag
     7561 ttgatttgtc gatttacaac attaacatct taacatgttt cagtcaaggt tgcaattatc
     7621 ttttcagatt tctggtcgga ttttttttac caaaggagac atcttgaaat tctttgaaaa
     7681 attgctgatt gttttttgtt ttacgcaaaa agcggatcag ttgttccgta tattctaatg
     7741 aatctccagt catatttttt cgaagtttcc aaatttcttc taattcttca cttggcatca
     7801 ataattcttc ttttcgggta cctgattttt tgatatcgat ggctgggaag atccgacgtt
     7861 cagataattc acgtgaaagt tgtaattcta gatttccagt tcctttgaat tcttcataga
     7921 tcacgtcatc catacggctt cctgtatcga ctaaagcggt cgctaaaatc gtcaaactgc
     7981 caccttcttc gatgtttctt gctgccccga aaaatttctt aggtttatac aatgcggctg
     8041 ggtcaatccc gccactcaat gtacgtccgc ttggtggaac gactaagtta taggcccgag
     8101 caagtctagt aatactatcc atcaagataa cgacatctcg tttatcttcg actaaacgca
     8161 ttgcgcgttc caatactaat tcagaaactc gagtatggtt ttgcggctgc agatcaaatg
     8221 tagaagatac cacgtcgcct ttcacgcttc gttctagatc cgttacttct tccggtcgtt
     8281 catcaatcag tagaacgatt aattctactt ccggatagtt ttcggtgata ccatttgcga
     8341 tttcttttaa tacagaagtt ttccctgctt ttggcggtgc gacgatcaat ccacgctgac
     8401 caaaaccgat tggagaaaag atatcgatca ttcgggtaga tagtctttgc ggtgttgttt
     8461 ccagttgaag ctgtctttgc ggataaagag gtgttaaggc tggaaaatgc ggacgttctt
     8521 ttgcttcttc cggatctttt ccattaacgc tttccacgtg catcaaacca taataacgtt
     8581 ccgattcttt aggtggacgg gcttttcctg ccactttgtc cccatttctc aaactgaatc
     8641 gtctgatttg tgaggcagaa atatagatat cttctgcact tggtccataa ttgattggcc
     8701 gtaaaaaacc gtaaccatct tgaccaacga tatccaatat tccttccata taataaaatc
     8761 cttgtttctc tgcttgtgca cgaatcacag ctagagataa ttccttttta ttcatcttac
     8821 tgtaataagg aattttaaat tctttggcat aactataaac ttcttttaac gttttgttct
     8881 ctagttctgc cattgtcaaa taatcgctca ttcagctccc acttcctcta tcatttcaat
     8941 tgatgcccct aaggctgtta gtttctcgat aatatgatca taaccgcgta agatataatc
     9001 tacattgtaa atcgtcgttg tcccttctgc caatagtcca gcaatcacta aacaagcacc
     9061 tgcccgaaga tcagaagcca cgacttctgt gccgtgaagt ttatttggac cattcacgat
     9121 gatcatattt ccttccacgg taatatcagc gcccattcgt gctagttccg gcacatggtt
     9181 cacacgtttc gcatagatcg tatcgatgat ttctgaagtg cctgtagtca tcaatagtaa
     9241 cgcagtaagt ggctgctgca gatctgtggc aaatcctgga taaggatagg tcatgatgtt
     9301 tgcgggtttt aattctttcg aggggtaaac ttcgatttca tcttcgctga ttttcatatg
     9361 gacaccaatt tcctgcaatt ttgctataaa gctttccaaa tgttcaaaga tcacattttt
     9421 cactttgata ccatttccta ctgcagctgc taatgctaga taggttcctg cttcgatgcg
     9481 gtcaggaatc atgaaatgcc gacagccatg taatgtttcg actccttcga tacggatcac
     9541 atctgtaccc gctcctcgaa cttttgcccc catattattc agtaaagttg ccacatctat
     9601 gatttccggt tctcgtgcag cgttttcaat tactgtctgt ccttttgctt ttacagcagc
     9661 taacatcaca ttgatcgttg ctccgactga aaccacgtcc atgaaaatcc gattgccacg
     9721 caatccttta ttttctgtcc gtaaatacat ggcaccatgt tcatttgtca cttcagcacc
     9781 tagagcttca aatcctttga cgtgtaggtc aatcggtcta gggcctaaat aacagccacc
     9841 tggcaggcct actaccgctt cgccaaattt accaagcagt gttcccataa aataataaga
     9901 agcacgcaaa ctattgattt ttccgtgcgg catcggaatc gatacgactt ctcgaggatc
     9961 gatttctagt atgttgttgg aaaaatggac agttgcgccc atgatctcta aaatttcaat
    10021 taatgaatga acatcttgta tgtccggcac accttccaag atcactgggg aatcagctaa
    10081 gattgcagct ggaatcaatg cgactgcact attttttgca ccgctgatcg ttacttcacc
    10141 agctaaaggt cggtttcctt tgattacgat ttttttcatc tgtttccacc ttgtttatta
    10201 atcttttttc atggatatgc ttgtatttta tcatagataa aaaaaagaaa cgagcatggt
    10261 ctgccgtttt aagtaaaaaa gaatggagag cgacttcaaa agtcgttctc cattcctata
    10321 tatcgtatct gttatgtgga tgcttacgct ttgttagctg aaccgaacca gtcgatatga
    10381 gatttagcat cttcaacgat tgctgcttta cctggagcta ataatttacg agggtcgaat
    10441 cctttacctt ctttatcttt accagcttcg atatatttac gagtagctgc agcaaatgat
    10501 aattggaatt cagtgttaac atttactttt gagataccca ttgagatagc tttttcgatt
    10561 tgatcttgag gaatacctga tccgccatgt aatactaaag gcatgtctgg acctacagca
    10621 tcagcaattg cttgtaagtg gtcaaaagct aatcctttcc agttttctgg atattgacca
    10681 tggatgttac cgataccgca agctaagtag tcgatacctg tagcaaccat ttgtttgcat
    10741 tcttcaatat cagctaattc gccgttaccg atgattccgt cttcttctcc gccgattgaa
    10801 ccaacttcac attcaactga gatacctttt gcatgtgctt tttcaacaac gtctttagct
    10861 aatttcaagt tttcttcaaa tggtagatgt gaaccatcga acattactga agtatagcct
    10921 aattcgatac attccaaagc agcttcgtaa tcaccatggt ctaagtggat agcaactgga
    10981 acagtgatgt tcattgcttc aactaaatca gtgattaaat ctttacagat tttgtaacca
    11041 cccatgtatt tagctgcacc cattgatgtt tggataagta ctggtgcttg ttttgcttca
    11101 gccgcttcaa ggattgcttg agtccattcc aagttgtttg tgttgaatcc gccgacagcg
    11161 taaccgcctt tacgagcagc ttttaaaaat tcagctcctg atactactgg cataataaaa
    11221 ttcctcctaa gttttgtaaa actaaatttg ttaagacaat tcttaaccaa cgaccctatt
    11281 ttaccaaaca tttcggtagt tttctactaa aatcaatgaa aatgaaaatt ttcaagaagt
    11341 tttttgcaaa aaataaaata tccaatagcg ctttaatcga aaaaagtctt ttgctttcta
    11401 tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag aaaattgctt
    11461 atccccttct cttgctactt ttctctttct atcaagttca aaaattattt tgtttcgtga
    11521 tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt tggacgagaa
    11581 atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag ctctacgatt
    11641 tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc ttcaaacaat
    11701 tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac aacatcggct
    11761 ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta taatccaagg
    11821 cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc aataatattg
    11881 tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac gacgttacgt
    11941 gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg aacgtcattt
    12001 tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc aaatccacca
    12061 ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct cgttaattct
    12121 tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga atgtttcaat
    12181 gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac aagcgcaatc
    12241 cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc tgtcatgtct
    12301 gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc ttgtgcttgt
    12361 aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac tgcttctggt
    12421 tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg ttgctctgta
    12481 cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac actgtgctgc
    12541 gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt tgtatggata
    12601 tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc aaggaatggc
    12661 aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc tgaatcagtc
    12721 attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg aataacttga
    12781 acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga atagattttt
    12841 cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc gtagtggccc
    12901 agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg ctgatatggg
    12961 ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt tacttttaag
    13021 ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc gattgatgaa
    13081 acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc tttttaattt
    13141 atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata agaataggga
    13201 gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta taatacaagc
    13261 tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt tttctttttc
    13321 gatatccttt gagaaattat ttcttaagtc ctttatgagt gcaaaaaaaa tttaacccac
    13381 ataataagct tgatataaac atagctaacg ttaaacctaa gcaaatcaga aaatcgttac
    13441 tgaaaaattt taagtgaaat aatagataaa aaaataaaac aaacattatg atagataata
    13501 tcccacctaa caaagaacta ctttttccta cttgcagcaa cagtttatat agcttatcgt
    13561 agaaacaatc tttttctttc acccttattt acctccttta cgtaaaaaac gtagtccaca
    13621 gttctcggat ttttggcata tcttttctat taaataatat caaaaacaaa acaattccac
    13681 taattttttc aaagatgacc tttttttcaa caaaagtgcc ttgtagggat tcattgaagg
    13741 taacgaatca cataagtaaa aaataaggag atgcgacaaa agtcgtgcct ccttatttct
    13801 aaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc atcaaacatg
    13861 agaagctgtt tttgaaatta tttcttgtga ttcggagtta aatggtaggc tcacaacctc
    13921 tggataaacg gt
//
LOCUS       DACEKQ010000076        13884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEKQ010000076 DACEKQ010000000
VERSION     DACEKQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.080"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW671_002064"
     CDS             complement(176..1462)
                     /locus_tag="IW671_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0899040.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW671_002065"
     CDS             complement(1455..2723)
                     /locus_tag="IW671_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0899041.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW671_002066"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW671_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0899042.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW671_002067"
     CDS             complement(3495..3941)
                     /locus_tag="IW671_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899043.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="IW671_002068"
     CDS             complement(4034..4279)
                     /locus_tag="IW671_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899044.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4393..4971)
                     /locus_tag="IW671_002069"
     CDS             complement(4393..4971)
                     /locus_tag="IW671_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0899045.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5048..5578)
                     /locus_tag="IW671_002070"
     CDS             complement(5048..5578)
                     /locus_tag="IW671_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0899046.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="IW671_002071"
     CDS             complement(5582..6442)
                     /gene="rlmB"
                     /locus_tag="IW671_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0899047.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6435..6830)
                     /locus_tag="IW671_002072"
     CDS             complement(6435..6830)
                     /locus_tag="IW671_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0899048.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6827..8239)
                     /locus_tag="IW671_002073"
     CDS             complement(6827..8239)
                     /locus_tag="IW671_002073"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0899049.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8236..8772)
                     /gene="cysE"
                     /locus_tag="IW671_002074"
     CDS             complement(8236..8772)
                     /gene="cysE"
                     /locus_tag="IW671_002074"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0899050.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9094..10554)
                     /locus_tag="IW671_002075"
     CDS             complement(9094..10554)
                     /locus_tag="IW671_002075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0899051.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10724..11881)
                     /locus_tag="IW671_002076"
     CDS             complement(10724..11881)
                     /locus_tag="IW671_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0899052.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11915..13285)
                     /gene="radA"
                     /locus_tag="IW671_002077"
     CDS             complement(11915..13285)
                     /gene="radA"
                     /locus_tag="IW671_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0899053.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13348..13875)
                     /locus_tag="IW671_002078"
     CDS             complement(13348..13875)
                     /locus_tag="IW671_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0899054.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3643 a         3030 c         2491 g         4720 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa
     4381 gatttttcta aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac
     4441 tctcccgtaa gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac
     4501 aaaatcaacg ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat
     4561 ttcgcaattt tcgattgctt cttttaataa aagatgctga atcgctaaag gttcttgcaa
     4621 tgtgatctct gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc
     4681 atctttttga tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt
     4741 gcttataatg cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct
     4801 caatgcctga tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct
     4861 taaataatag gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc
     4921 aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt
     4981 cctccataaa ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa
     5041 cgatatgtta tttatttttt ctcattaaat cgtccaattt ttgggacaat ttttttaact
     5101 gctcttcatt ccatggagaa ttacgtcgga aatcaataaa atgcatatct ttattttctt
     5161 gggctattgt tttttccgtt ttttttatcg tcttatataa ttcataagca gaagttcgta
     5221 atgctccttg ggaaaagatc atccactgtt ctgctaaatc gcttgtcgcc actgtcactt
     5281 gtgtcaaacg gtcattcagc tgaccagcga ttctctcgat ataactgtca gccgtttcgt
     5341 cttctttcgt aaaaacaaca tccaactggt atttcttata attttgttgg atgcccggaa
     5401 ctagttgggc atcgaagaca acgatgactt ccattccttc atatttcgca taatttgata
     5461 aacgatgtaa aagagcttct cttgcatccg ccattttgtc ctgcttttta agctggacaa
     5521 gttccggcca cgaaccaatc atgttgtagc cgtctacaat caacagctgt tttttcatgg
     5581 tctactcccc ctgacgttgg gaaaaagcct gatacataag caaaccagca gcaacactgg
     5641 catttaagct ctggacgtgc ccggtcatcg ggatcgtcag caattcatcc atttcttttt
     5701 tcaatcctgg actcattcct ttgccttcat taccaatgac taaagcaatt gatcctttgg
     5761 cattccattt ttggaagttt gtgccggtca tatctgtgcc aaatacccaa aatcctgttt
     5821 ctttgagctg ttgcacagct tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga
     5881 ctgccccagt ggaagtcttt gttacgatag gtgtgattcc aactgctcgg tgttttggta
     5941 tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg
     6001 gatcttcgat gctatccaaa atcaaaaaga aggggtcttc ttttttctct tttgttcgtt
     6061 caatcagttg gtcaagggat aaatattcat aaggtgtaat cgcaagaacg actccttgat
     6121 gcacgccgtg atcgctcaaa gtttctaatt tttgtttagg aacccatttg actggcacag
     6181 cttgttcacg tgcgatttct ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa
     6241 gaaagagttt attccctcgc ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga
     6301 cgaaattatc ttctaatgct tcttcatttt gatcagaaac tgactgattt cgtccagttt
     6361 gctttttatt tgaatatcta ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt
     6421 tgtttgttct ttctttaccc atttttttca ccaactttct gaatacacca gcggatcaat
     6481 tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct
     6541 gttgcgaccc gatacgtcgt aacatcagca ttttttgcgc tcgtatggct tttagcattt
     6601 cttcctctgc gataataaag ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc
     6661 tgaatcaaaa aggcttgtgc attcgcagaa acataattcg ttgccgcacg atgcagctta
     6721 ttgggttttg tttctccttg atcaatcagg tggtcacgaa catagatttc gtaaatcgca
     6781 tctccaacat aagccaaggc taaaccgttc aattgtgtgt aatctctcat gcttctcttc
     6841 tccatcgggt tccttgcggt gtatcttcta ataagatccc tttttctttc aacaaatcac
     6901 gaatttcgtc gcttcggaca aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca
     6961 attgttcaat ctcttcatct aataactctt ctgctgtaaa taaaataccg aagatcgaaa
     7021 gccattgcga gaataaagct tctccttgtg ccaagacagc ggcagaaact ttcggctggt
     7081 ctagataagt gttcagccac ttagcaagct catagaccac tgtgatccca tttgctgcat
     7141 taaaatcatc gtccatttct tcgatgaatc gctgttctaa attttctaac aaagcgattt
     7201 tatccgcatc atctgccaaa ttctctgagg cagtttcttg gcggaatttc aaattgtcaa
     7261 acgtattctt cagtcgttgt aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc
     7321 ggatcggacg acggtattgt gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt
     7381 ttttgatcat ttcatgtaca gtgatgaaat tccctagaga tttactcatt ttttcatcgt
     7441 cttcgccaat cgttacatag ccattgtgca tccagtaatt agcgaatttt ttccctgttt
     7501 tcgcttcact ttgtgcgatt tcattttcat gatgaggaaa ttctaaatct tgcccgccac
     7561 catggatatc gatcgtttct cctaaatgtt ttgttgccat aacggaacat tcaatatgcc
     7621 aaccgggacg tcctttgccc catggtgaat cccaagaaat ctcgccaggt tttgcttgtt
     7681 tccataaagc gaaatctaat gggtcttctt tttgctgctg ttcttcccct gtacgctgac
     7741 ttgctccgac ttccagttca tcgatggatt ggtcacttaa tttcccataa ttttcaaatt
     7801 tacgggtacg gtaatacaca tcacctgaag attcataagc atagcctttt tcaaccaaca
     7861 cttgaataaa tgctaaaata tctggcatat gatccatgac tcttggatgc agtgtagcag
     7921 gctcgacatt caacgctttc gtatcttctt caaatgctcg gatgaaacga tcagcaactt
     7981 ctggtgctgt aatacctaat tcgtttgctg tacggatgat tttatcgtct acatccgtga
     8041 aattcgatac gaaatcgact ttatagcctc gatattcaaa atagcgtcga attgtatcga
     8101 aagcaatggt gctacgagca tttccgatat ggatataatt atacacagtc ggtccgcaga
     8161 catacatacc gacatgtccc ttttgtatag ggatgaattc ttcttttttt ctggtcaatg
     8221 tattgtagat ttttatcatt ctgatctcct cactttttct cctttgattc gaacgacttt
     8281 tgctggtact cctaccgctg tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc
     8341 accgattttg gcgttttttc cgatcgttac cggtcctaat atctgcgcat tcgcagagag
     8401 catggctcct tgtttaacag ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa
     8461 tgttacgcca tggaataaaa tcacatcgtc ctcgatttct gcggtttcgc caatgacgat
     8521 tcccattcca tggtcgatga acacgccagt tccaatcgtt gctcctggat ggatctcaac
     8581 accagttagg aaacgccaga attgagcatt gatcttcgcc aataaaaaga gccggtgctg
     8641 atataaaaaa tgagagaaac gatgccaaaa caaggcatga aaaccagggt atgtcaaaac
     8701 gatctcagct gtcgaacgtg cagccggatc gtttctcttt gctgcatcaa tcgctcgggt
     8761 aaaccaactc atgactttcc actcctttcg taagaatttt atgtaggaaa tcatcttctt
     8821 ataaagaaaa gaagcgcctt ttaaataagt ttccttatca aaagacgctt tccgcgtggt
     8881 tccacttttc ttcataatca atgattatct ctgacagcta acgcctgtta tacgttcttg
     8941 gctactttgt ttcctataaa acagtttttc ctgttttctg tgtttcgcca agaccactcc
     9001 cagaggcatt tcggcaacca ttcattgata ccttgcacca cccggtatct ctcttgtaat
     9061 gatcagctgc ttactttttt ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc
     9121 taatgatttt tcacgaccaa gcaattcgat cgtttcaggc aattctggac catgcatttg
     9181 tcctgataca gctacacgga ttggcatgaa aagatttttt ccttttacgc cagtttcttt
     9241 ttgaacagct ttgatggcag ctttgacagt tggagcatct actacttcca tttgttctag
     9301 ctgtgctttg aatgcgtgga gaacagtagg aactgtttca ccagcaagta cttcttttgc
     9361 tgcttcattc aatacaggat gttcattgaa aaatagcgta gacagatcaa cgatttctgc
     9421 agcatagctc atttgaggtt gatacaggct aacgatcttc ttcacccatt cgattgtctt
     9481 ttcatctggg ttttctgcta cacgtccatc agcgattaga taaggcagac acatgtcagt
     9541 aaatgcatct agatccattg ctttgatgta atggttgttt acccattgca gttttttcgc
     9601 atcgaaagct gcgggtgatt tacttaggcg ttttggatcg aacattttga tcaattcttg
     9661 gtgttcgaag atttcgtctt cgcccacagg tgaccagccc aataatgcaa taaagttgaa
     9721 cattgcatct ggtagatagc ctaattcgcg atattgttcg ataaattgca agattgattc
     9781 gtcacgtttg cttagttttt tacctgtttc actgttgatg atcaatgtca tatgtccgaa
     9841 ggttggtggt gtccagccaa atgcttcata gatcatcagt tgttttggtg tattggcaat
     9901 atggtcatct ccacgtaaaa cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc
     9961 aaaattataa gttggcatac catcgcgttt ttgtatgaca aagtcgccac cgatattatc
    10021 ggattcgaac gaaatagcgc ctttcaccat atctgtaaat gcgtattctg tattgcgagg
    10081 cacacggaaa cgtacaacag attctagacc ttgtgcttct ttttctgctt gttcttcagg
    10141 tgtcaagttc gcacatgttc ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc
    10201 ccgttctgct tctaattctt cttctgtgca atagcatttg tatgctcgat tgctagcaag
    10261 cagctgatca atcagcggtt gatagatttc tttacgttca gattgacggt aagggccgta
    10321 ttctccagga ttttcaggag attcatccca attcattccc aaccaagcaa ggttttccaa
    10381 ctggcttttt tcgccatctt cgatattacg tttttggtca gtatcctcaa tacgaataat
    10441 aaagtctcca tcattgtggc gggcaaacaa ataattgaat aatgcagttc ttgcatttcc
    10501 aatatgcagg tgcccagttg gacttggtgc atagcgtacg cgtactttcg tcattttctt
    10561 cctcttcttt caaactagac tttgactaac agcaaaaatt ccgtcagtta cggcttctca
    10621 aatatcattt atttaaagcc actcagcaca aattttacct tttctaaggt aaatagtaaa
    10681 gccaactggt tgaatttcct taccagttgg ctgaatttct tttttatttt gattgtttag
    10741 tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa
    10801 agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat
    10861 tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc
    10921 cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga
    10981 cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt
    11041 cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca
    11101 atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga
    11161 cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca
    11221 cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag
    11281 ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa
    11341 tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta
    11401 acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa
    11461 aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga
    11521 tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag
    11581 gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa
    11641 gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata gctgagatat
    11701 gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa
    11761 atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa
    11821 atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca
    11881 tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt
    11941 cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt
    12001 tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt
    12061 caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg
    12121 ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa
    12181 tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa
    12241 gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa
    12301 gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct
    12361 ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt
    12421 ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat
    12481 gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa
    12541 tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta
    12601 acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta
    12661 tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt ttctcttact tggctgacac
    12721 ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat
    12781 aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata
    12841 gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc
    12901 tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt
    12961 gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg
    13021 gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt
    13081 cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg
    13141 tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc
    13201 cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa
    13261 attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt
    13321 aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg
    13381 ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat
    13441 acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat
    13501 atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt
    13561 caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgca aaaattcatc
    13621 ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt
    13681 ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc
    13741 tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg
    13801 atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa
    13861 tcctcttttt ctcaccggtt ttcc
//
LOCUS       DACEKQ010000077        13726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEKQ010000077 DACEKQ010000000
VERSION     DACEKQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.081"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(35..667)
                     /locus_tag="IW671_002079"
     CDS             complement(35..667)
                     /locus_tag="IW671_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899055.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(815..1357)
                     /locus_tag="IW671_002080"
     CDS             complement(815..1357)
                     /locus_tag="IW671_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0899056.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1492..1881
                     /locus_tag="IW671_002081"
     CDS             1492..1881
                     /locus_tag="IW671_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899057.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1885..2649
                     /locus_tag="IW671_002082"
     CDS             1885..2649
                     /locus_tag="IW671_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0899058.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2778..3725
                     /locus_tag="IW671_002083"
     CDS             2778..3725
                     /locus_tag="IW671_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0899059.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3855..4595
                     /locus_tag="IW671_002084"
     CDS             3855..4595
                     /locus_tag="IW671_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899060.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4886..5554)
                     /locus_tag="IW671_002085"
     CDS             complement(4886..5554)
                     /locus_tag="IW671_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0899061.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5688..5975
                     /locus_tag="IW671_002086"
     CDS             5688..5975
                     /locus_tag="IW671_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899062.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6391..7929
                     /locus_tag="IW671_002087"
     CDS             6391..7929
                     /locus_tag="IW671_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0899063.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7926..8891
                     /locus_tag="IW671_002088"
     CDS             7926..8891
                     /locus_tag="IW671_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899064.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8878..10200
                     /locus_tag="IW671_002089"
     CDS             8878..10200
                     /locus_tag="IW671_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0899065.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10263..10367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10441..11184
                     /locus_tag="IW671_002090"
     CDS             10441..11184
                     /locus_tag="IW671_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899066.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11199..12191
                     /locus_tag="IW671_002091"
     CDS             11199..12191
                     /locus_tag="IW671_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0899067.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12203..12940
                     /locus_tag="IW671_002092"
     CDS             12203..12940
                     /locus_tag="IW671_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899068.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12930..13652
                     /locus_tag="IW671_002093"
     CDS             12930..13652
                     /locus_tag="IW671_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0899069.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         4649 a         2309 c         2818 g         3950 t
ORIGIN      
        1 taaaactaaa aaagcattta tgaagtcgta atagtcaggc ttcaaaatgc tttttatttt
       61 gtttgttttt tctttgccac ataaatacac ctagactgac gatgaagagc atatccaaca
      121 gatttcctgc gacttggatc gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa
      181 aggaaaacaa gctgtaaata aaacaggtga caggaactaa tcgaaagaag agcggacatt
      241 tcaattggct aaaaatgagt gaaaaaataa aataagtaaa aatggataaa atcacgacta
      301 aaccattcat gataaagaag aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta
      361 aaaaagaaaa cgttttaaat gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat
      421 gtcgatgaaa aaaataggca ccgaaataaa cagaaaacag taagccgcaa agagaagaca
      481 aaatcaaaaa aaacggattt tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc
      541 agcctaatgc actagaaggc gcatgtgagg tcttaaggta tgaataacat agaaaaaata
      601 agatcgtcaa agttgcaaaa aaagagccta aatgtaaaaa attagtggtt ctacgttgaa
      661 tttccataat atctccaaat taaattgata agtatataaa gtataataac aaattgatga
      721 gtatatgtcg atgtttttta atcaaaataa taaataaaaa ctgtctaaca tttaaaattt
      781 aatttagata ttagacagtt tttaagtttg tgagttattt tccttgtcgt tttctcactt
      841 ttttagtcca gaaaccacct gaaatatatt ttggctcata agaaatgata aaagcacgct
      901 cttcgatttc tttgatcagg cgatacagtt cacgttcgct ttttctagga gaaagtattt
      961 cgagaactag ccgctcacct tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt
     1021 gttctctcag tatacccggc aaacttttat cttctgcgct atttgctggc aaaatcgttg
     1081 tgaccatgat gtatcctaat gccaatttgt cttcaatcaa aattccgaca ctgataccta
     1141 ctgcgtaacc taacgcatag acaaataaat tcagtggatt atctaaacga tctaaaacaa
     1201 gggataagcc gacgatataa atcgtgatct cgaccatact gacaagtgga gcaatcagtc
     1261 gatagccttt cattgtcaac atgaaacgta acgtgttcag agtgatataa gcaaaattaa
     1321 tgaggaagat catccccaac atttttggat caatcatttg taaaactcct ttcatttata
     1381 taacaacaat atcaaaaaaa atcgtaagtg aagaaaaatt gaaactttat acatacaaaa
     1441 aaatgataaa atgattataa tagaattata aagataggtt aggtgattaa aatgaaaacg
     1501 gtcaatcgtg tgttttgtgc cagccacgtg ctaagtctcc ttctcagtat atacttaggt
     1561 gtctattcga ttcaacagcc aattgaggta gagaacattg tttttcagat gtcgctgaca
     1621 gatggtttaa ttttaagcgt ttttttcttt ttgatactgt ttattggtaa tattttcggt
     1681 agcgttgcca gttttttgaa tttttctatt tatccactcc tttcgctagc attaggagtg
     1741 gtgggacttt gttctctgtg cttatttccc gagccattca gtcctgcatt tatattattt
     1801 ggtacactga acctgttcca gacaactgtt ggagcatggt tgctatggcg atcaggaaat
     1861 ttaatgaaaa taggtgagta acaattgaat ccaattatat tgaaacgttc tgtccatcat
     1921 attccagtgt tagaagttgt tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc
     1981 tattatcacg gatggcagac aagtaaggag ttagttttga cgcaaggaag aaaaattgcg
     2041 cagcagggca ttcgtgtgat cctgccagat gcgatgaatc atggggagcg caaacagccg
     2101 gtttcttcta ttccatccta tacattttgg gatagtatct acggaaatct gtttgaattt
     2161 gacacactta tcaagcattt agaaaaaaga ggtcttctac atgatcagct tgcagttggc
     2221 ggggtttcga tggggggaat gacaacatgc gccttgttgg ctaaacatcc tcatatagta
     2281 gcgggcgtgt gtttgatggg ttcccctgca cctttggaat atggaaaaga aatcgtaagg
     2341 cgcgcatcgg aatataatta tgtcgtacca aaagactatt ttgatttgat ttcatgggtt
     2401 aagagctatg atttgtcatt gcagccggaa aagttagctg gacgtcctct gttcttttgg
     2461 catggacaag ctgacgaaaa aatcccttat atacaaacgg aacgatttgt agaaaaaaat
     2521 cggcataagt cttacggaaa agaaattgtt tttcgttcct caaaaacaca gcgacatatg
     2581 gtacaaatac cactaatgga agaagcttcc acttttttag caaaaaattt gataaaaaag
     2641 tataaataat tgaaacggaa aatcactcat gacgatagag gattttccgt tttttatttt
     2701 gggctataat aaactctgtt aggaaaattt ggtataattg atagtaagaa tgacaacatg
     2761 ttagaaagga agagagcatg cccactttag cagatgttgc aaaacgtgcc aatgtttcaa
     2821 aaatgacggt ctctcgagtc atcaatcatc cggaacaggt gactgacgaa ttgaaagagt
     2881 tagttttctc agcaatggct gaactggatt accgtccaaa tattgctgca aaagcacttg
     2941 tcacgaatcg gtcgcagatc atcaaattat ttatactgga agaaatcgac acaactgagc
     3001 cctattacat gaatttgctg atgggaatcg ctaaacggat cggtaaagcg cattattctc
     3061 tccaattagt tacaaatgat gccttcgacg tcggttcttg tgatggatac atcatcacag
     3121 gtgtcagaaa aaaagacttc gattggatcg accgtttgga aaaaccagtt gtcttgtttg
     3181 gggaaaattc tgaaggatat gattatgtag acagcgataa tgaatatggt acagcaaaag
     3241 caacgcaata tgccttagaa agagattatc aaacgattat ttatatcggt atcgaagtgg
     3301 acgagccttt tgaaatttca agggaaactg gttatttatc tgtgatgaag gaagttgatc
     3361 ttcctagtaa aatcttgcgg tttccaaatc attcgacaga agtagaaaga tatatgacag
     3421 aaaactgggc agattttaca gataaaactt gttttgtatg tagttcagat cggctagcac
     3481 taggagcagt aagagggatt ataaatgctg gaggacactt gcccgaagaa tttggtgtga
     3541 ttggatttga tggggtattt ttagaccaaa tcagttcacc taaattgaca acgatcaagc
     3601 aagatattat acgtatgggt gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa
     3661 aaggggcgag tcaaggttat cgtcgttttt ttcctgaact agttatcaga gagacaacga
     3721 attaagtctt attactatat atatatttat gaaattcatc gctattttct taaaataatc
     3781 agcgcattgc tactgaaaac aatgaatctg tgctattatt gagaaaagtt ccatttttga
     3841 gaaggatggt ttgaatgaaa gaaatcagac gaaaacattt ccgttttcct gcgtacgatg
     3901 atgagttagg cgtaaaatta accaacgaca agaagcgagt attatttgac ggacaagaag
     3961 atcttttgac tgaacagaat cagtccccgt ttgaacagat gaaagaattt catttcacga
     4021 acacgaacac cgagaaaatc cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc
     4081 cgcgtaagga aaatttggaa aagcataaag gaaaattacc ggattatacc ggccattacc
     4141 aatcagaaat cacatcgaca ggacgaaaaa aactttttgg tgattcctct cgttctgtca
     4201 gacaaagtga aacaaggaca gagtccaagc atactacacg aaacgcatct gttgacacga
     4261 aggtgcggga gcgttcttat tttgttccta aatatgtacc cgcatcgatt atcccagacc
     4321 ctagagaaag caaaatttct gaggatcatt taatcgaatc gatgaaaaaa acggatgact
     4381 cttatctgtt gttcgataca gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt
     4441 ctgtacgaac attcaatcga acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa
     4501 aatcgaaaaa aagccatggt atcttagaac gctcattaca aggattgatc gatgatcaag
     4561 gaaactcttt gggagaaaat agctatttta aataagtgat atacaaattt atattcggct
     4621 acagttggaa aggaaacgct gcggcgtttc cttattttat actctatccg aaagtttata
     4681 tcgtatcctc aaattttttt gagtttaagc gaattttttc tcccaataag taagaaaata
     4741 atggcaaaat aaaaaaacaa actattttcg cagatcgttt gcaaaaatag tttatttttt
     4801 tataaagaag attttactga ataatatcat tttagtttac ataatgatat ttctgtaaat
     4861 taaataaagt agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt
     4921 caaatgtttc tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat
     4981 acttcacttt attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat
     5041 gtgtgagatc caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac
     5101 ttttgccaat cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc
     5161 ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca
     5221 tcaaactatt gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat
     5281 gtaaaaagaa aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa
     5341 aaagaaacaa tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag
     5401 cgaatccaaa aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct
     5461 tagatggaat attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc
     5521 cgcacaattc tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt
     5581 ttcagcaact tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa
     5641 attatttttg gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag
     5701 acttattgac aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg
     5761 ctcaagaaca aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta
     5821 tctttttatt tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga
     5881 tgaagaaccg tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg
     5941 gagtgaaaga gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt
     6001 atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa
     6061 tattcgttga acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg
     6121 tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac
     6181 aatttcgtat agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc
     6241 gactttgttg cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt
     6301 tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta
     6361 tacttaaaga ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa
     6421 agaaaaggcg agttgctgga agctgcattt caacatctgt ccatttctct catttctttg
     6481 ctgattgcag cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg
     6541 gcagaagtct tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta
     6601 ggtttactta tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt
     6661 tacgctttgc ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct
     6721 attgaagaag cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag
     6781 cttcccatcg cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc
     6841 ggtacggcta ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta
     6901 ggtattgacc gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt
     6961 gcaattatat tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc
     7021 attactttag cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg
     7081 tttaaaaatg aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc
     7141 caaatgtata aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat
     7201 tttggcaaga cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct
     7261 gagttcacag gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct
     7321 gatttgacaa aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat
     7381 atgacattat taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct
     7441 tttgcgaacg aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc
     7501 aaagcgggat tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa
     7561 gcatatggct tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca
     7621 atcaataatg gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag
     7681 tacgatctag tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg
     7741 ctgatgaaaa aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta
     7801 agcggaaaaa taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag
     7861 aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa
     7921 gatcagtgac taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg
     7981 tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt
     8041 ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag
     8101 gagacatcta cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga
     8161 atatgggata tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata
     8221 ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg
     8281 aacttcttca aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt
     8341 tgtctggagg agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg
     8401 tgattttgat ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag
     8461 aattggtttt gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata
     8521 tggaagaagc aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc
     8581 aatgtgacac accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct
     8641 ttgatgaacc gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt
     8701 tcttaaatga aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag
     8761 aagtatattc gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta
     8821 ttgggtcgct gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg
     8881 aaaacatatg atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga
     8941 ttgaaagaag caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt
     9001 cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg
     9061 gtaaaacaat tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt
     9121 caaaaattca aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca
     9181 gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa
     9241 gtcaacgaag aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg
     9301 atcttaccag tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct
     9361 gtactgccta aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg
     9421 attgccaatg ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa
     9481 gaatttgagg cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag
     9541 tttgcttttg gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc
     9601 ggaaaagaga cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat
     9661 acagaatctt tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc
     9721 aatgattatc tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg
     9781 aaaaccccaa aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata
     9841 acggatgcaa caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt
     9901 cctaaacttg ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta
     9961 gagatggatc ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct
    10021 gaattgacct ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca
    10081 gacgaattga tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg
    10141 gatagctata tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa
    10201 tcagcatgat aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg
    10261 aatacctgtt tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat
    10321 tgaacctgtc agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac
    10381 tatgtcgtgc ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa
    10441 atgaagaaaa atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt
    10501 gttgtcatag tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct
    10561 gcattagcgg cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca
    10621 gtgcttggtt tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat
    10681 tggaagtttt tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc
    10741 atggtgttag ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc
    10801 cttgttgtct tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa
    10861 gtatcaaaag agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat
    10921 cgaatatata aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct
    10981 acttattgtg tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg
    11041 tattacatga tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt
    11101 tgtgtcattc tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat
    11161 tatcgtatgc tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat
    11221 taccgctact gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag
    11281 aaataaagga tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata
    11341 ctggcatcta cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa
    11401 tcatcgaacc aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat
    11461 ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag
    11521 tcaaagcaat tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca
    11581 gtaacatcca ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa
    11641 gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga
    11701 caatggtagg aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat
    11761 ggtactttga aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata
    11821 tcccattgaa agaattagat gaacggttag attactatac tccggtaatt attacgaatg
    11881 atcaactgat caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag
    11941 gctatcagga agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa
    12001 aagaaaatga tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg
    12061 atgatcctga gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta
    12121 tgcaggaaaa cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt
    12181 ttataaaata ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga
    12241 aaacctgaaa atattagaag acatccattt cactatccct aaagatacct ttacatcgat
    12301 tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc
    12361 tatgacttct ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt
    12421 tagttatatg ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac
    12481 gttatatcaa aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga
    12541 tatctttggg ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa
    12601 gcagcgaacg gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga
    12661 accatttgga gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct
    12721 gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta
    12781 tttatcagac aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca
    12841 agtgcctgaa aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac
    12901 gatttatcag ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg
    12961 ggagtcagag tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg
    13021 attcgccaga agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa
    13081 gttacggtat ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt
    13141 tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga
    13201 tgactacaca aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac
    13261 ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc
    13321 ctaatcacta tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt
    13381 taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag
    13441 ctaaagcagt cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt
    13501 gggctttagg ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa
    13561 agacaatcgc tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa
    13621 acttggaacg tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc
    13681 acctcttttt ggctttcttt gtataaaagt aaactgtttg attaat
//
LOCUS       DACEKQ010000078        13692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEKQ010000078 DACEKQ010000000
VERSION     DACEKQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.082"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW671_002094"
     CDS             complement(221..1177)
                     /locus_tag="IW671_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0899070.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW671_002095"
     CDS             1281..1883
                     /locus_tag="IW671_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0899071.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW671_002096"
     CDS             complement(1971..3470)
                     /locus_tag="IW671_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0899072.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW671_002097"
     CDS             complement(3486..4010)
                     /locus_tag="IW671_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899073.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW671_002098"
     CDS             4308..5057
                     /locus_tag="IW671_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0899074.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW671_002099"
     CDS             5048..5833
                     /locus_tag="IW671_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0899075.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW671_002100"
     CDS             5802..6518
                     /locus_tag="IW671_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0899076.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW671_002101"
     CDS             complement(6620..7639)
                     /locus_tag="IW671_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0899077.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW671_002102"
     CDS             complement(7658..8716)
                     /locus_tag="IW671_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0899078.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IW671_002103"
     CDS             complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IW671_002103"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0899079.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10160..10996)
                     /locus_tag="IW671_002104"
     CDS             complement(10160..10996)
                     /locus_tag="IW671_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899080.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11180..12397)
                     /locus_tag="IW671_002105"
     CDS             complement(11180..12397)
                     /locus_tag="IW671_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0899081.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12780..12863
                     /locus_tag="IW671_002106"
     tRNA            12780..12863
                     /locus_tag="IW671_002106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12814..12816,aa:Leu,seq:gag)
     gene            complement(13103..>13692)
                     /locus_tag="IW671_002107"
     CDS             complement(13103..>13692)
                     /locus_tag="IW671_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899082.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4139 a         2794 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
     8941 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
     9001 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
     9061 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
     9121 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
     9181 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
     9241 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
     9301 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
     9361 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
     9421 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
     9481 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
     9541 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
     9601 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
     9661 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
     9721 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
     9781 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
     9841 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
     9901 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
     9961 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
    10021 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
    10081 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
    10141 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
    10201 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
    10261 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
    10321 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
    10381 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
    10441 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
    10501 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
    10561 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
    10621 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
    10681 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
    10741 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
    10801 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
    10861 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
    10921 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
    10981 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
    11041 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
    11101 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
    11161 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
    11221 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
    11281 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
    11341 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
    11401 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
    11461 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
    11521 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
    11581 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
    11641 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
    11701 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
    11761 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
    11821 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
    11881 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
    11941 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
    12001 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
    12061 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
    12121 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
    12181 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
    12241 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
    12301 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
    12361 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
    12421 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
    12481 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
    12541 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
    12601 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
    12661 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
    12721 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
    12781 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
    12841 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
    12901 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
    12961 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
    13021 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
    13081 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
    13141 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
    13201 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
    13261 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
    13321 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
    13381 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
    13441 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
    13501 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
    13561 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
    13621 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
    13681 tgtctaaaaa aa
//
LOCUS       DACEKQ010000079        13685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEKQ010000079 DACEKQ010000000
VERSION     DACEKQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.083"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            46..2211
                     /gene="acm"
                     /locus_tag="IW671_002108"
     CDS             46..2211
                     /gene="acm"
                     /locus_tag="IW671_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0899083.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2483..2968)
                     /locus_tag="IW671_002109"
     CDS             complement(2483..2968)
                     /locus_tag="IW671_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0899084.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3448..4089
                     /locus_tag="IW671_002110"
     CDS             3448..4089
                     /locus_tag="IW671_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0899085.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4108..4743
                     /locus_tag="IW671_002111"
     CDS             4108..4743
                     /locus_tag="IW671_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899086.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4761..5576
                     /locus_tag="IW671_002112"
     CDS             4761..5576
                     /locus_tag="IW671_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0899087.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5895..7331
                     /locus_tag="IW671_002113"
     CDS             5895..7331
                     /locus_tag="IW671_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0899088.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7520..9967
                     /locus_tag="IW671_002114"
     CDS             7520..9967
                     /locus_tag="IW671_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0899089.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10197..11264
                     /gene="rlmN"
                     /locus_tag="IW671_002115"
     CDS             10197..11264
                     /gene="rlmN"
                     /locus_tag="IW671_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0899090.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11457..12560)
                     /locus_tag="IW671_002116"
     CDS             complement(11457..12560)
                     /locus_tag="IW671_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0899091.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13113..13661
                     /gene="aac(6')"
                     /locus_tag="IW671_002117"
     CDS             13113..13661
                     /gene="aac(6')"
                     /locus_tag="IW671_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0899092.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4683 a         2282 c         2902 g         3818 t
ORIGIN      
        1 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
       61 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      121 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      181 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      241 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      301 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      361 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      421 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      481 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      541 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      601 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      661 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      721 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      781 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      841 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      901 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
      961 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1021 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1081 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1141 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1201 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1261 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1321 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1381 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1441 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1501 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1561 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1621 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1681 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1741 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1801 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1861 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1921 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     1981 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2041 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2101 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2161 atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta gttagaatat
     2221 aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt ataatcgaaa
     2281 aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta
     2341 actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat
     2401 tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg
     2461 aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa
     2521 caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat
     2581 gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct
     2641 ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag
     2701 gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt
     2761 ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg
     2821 atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg
     2881 acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat
     2941 aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat
     3001 cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa
     3061 atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa
     3121 gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga
     3181 aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt
     3241 tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga
     3301 tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg
     3361 aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat
     3421 ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta
     3481 ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca
     3541 ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt
     3601 caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt
     3661 tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt
     3721 atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct
     3781 caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa
     3841 atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac
     3901 ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg
     3961 aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat
     4021 cttacattga ctggtattct aacaatcgta atgaaacaag ttgaaggaaa attaaattat
     4081 taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt
     4141 ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct
     4201 atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa
     4261 agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa
     4321 atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat
     4381 atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac
     4441 accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat
     4501 ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg
     4561 aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag
     4621 caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc
     4681 catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat
     4741 taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc
     4801 ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca
     4861 acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag
     4921 ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat
     4981 atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca
     5041 atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag
     5101 tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag
     5161 gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg
     5221 gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg
     5281 ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg
     5341 gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag
     5401 acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt
     5461 cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg
     5521 aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa
     5581 taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct
     5641 gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt
     5701 caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
     5761 caaaattttt cttactactc gaaactaagc gactttttca caacctcctt tacatatttt
     5821 tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg
     5881 aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg
     5941 gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat
     6001 cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt
     6061 tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc
     6121 tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag
     6181 tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata
     6241 ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata
     6301 atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg
     6361 aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt
     6421 cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg
     6481 aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag
     6541 aaatgcccgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta
     6601 atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg
     6661 atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat
     6721 gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc
     6781 ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg
     6841 ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa
     6901 tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc
     6961 cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga
     7021 ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg
     7081 actatcgaat caattactta caaagccacg tcaaagccat caaagaagca atttcggatg
     7141 gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg
     7201 gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt
     7261 tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag
     7321 agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt
     7381 cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa
     7441 gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg
     7501 atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac
     7561 gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt
     7621 tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat
     7681 tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc
     7741 cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg
     7801 ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt
     7861 tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt tgttagccag
     7921 tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct
     7981 ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt
     8041 actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat
     8101 ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg
     8161 aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg
     8221 cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt
     8281 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
     8341 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
     8401 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
     8461 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
     8521 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
     8581 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
     8641 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
     8701 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
     8761 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
     8821 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
     8881 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
     8941 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
     9001 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
     9061 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
     9121 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
     9181 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
     9241 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     9301 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     9361 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     9421 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     9481 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     9541 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     9601 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     9661 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     9721 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     9781 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     9841 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     9901 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     9961 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
    10021 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
    10081 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
    10141 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
    10201 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
    10261 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
    10321 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
    10381 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
    10441 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
    10501 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
    10561 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
    10621 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
    10681 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
    10741 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
    10801 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
    10861 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
    10921 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
    10981 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
    11041 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
    11101 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
    11161 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
    11221 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
    11281 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
    11341 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
    11401 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
    11461 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
    11521 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
    11581 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
    11641 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
    11701 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
    11761 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
    11821 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
    11881 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
    11941 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
    12001 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
    12061 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
    12121 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
    12181 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
    12241 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
    12301 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
    12361 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
    12421 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
    12481 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
    12541 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
    12601 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
    12661 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
    12721 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
    12781 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
    12841 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
    12901 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
    12961 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
    13021 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
    13081 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
    13141 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
    13201 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
    13261 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
    13321 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
    13381 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
    13441 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
    13501 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
    13561 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
    13621 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
    13681 aagta
//
LOCUS       DACEKQ010000080        13487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEKQ010000080 DACEKQ010000000
VERSION     DACEKQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.084"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(164..388)
                     /locus_tag="IW671_002118"
     CDS             complement(164..388)
                     /locus_tag="IW671_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0899093.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="IW671_002119"
     CDS             complement(586..2166)
                     /locus_tag="IW671_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0899094.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="IW671_002120"
     CDS             complement(2567..3943)
                     /locus_tag="IW671_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0899095.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="IW671_002121"
     CDS             complement(4071..5189)
                     /locus_tag="IW671_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0899096.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="IW671_002122"
     CDS             5331..5765
                     /locus_tag="IW671_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899097.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="IW671_002123"
     CDS             complement(5843..6376)
                     /locus_tag="IW671_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0899098.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IW671_002124"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="IW671_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0899099.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IW671_002125"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="IW671_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0899100.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="IW671_002126"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="IW671_002126"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0899101.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="IW671_002127"
     CDS             complement(10101..10679)
                     /locus_tag="IW671_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0899102.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="IW671_002128"
     CDS             11001..11471
                     /locus_tag="IW671_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0899103.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="IW671_002129"
     CDS             complement(11579..12805)
                     /locus_tag="IW671_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0899104.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3958 a         2719 c         2248 g         4562 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaa
//
LOCUS       DACEKQ010000081        13335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEKQ010000081 DACEKQ010000000
VERSION     DACEKQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.085"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IW671_002130"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IW671_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0899105.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IW671_002131"
     CDS             complement(738..1460)
                     /locus_tag="IW671_002131"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0899106.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IW671_002132"
     CDS             complement(1596..2477)
                     /locus_tag="IW671_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0899107.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IW671_002133"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IW671_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0899108.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IW671_002134"
     CDS             3717..4196
                     /locus_tag="IW671_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0899109.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IW671_002135"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IW671_002135"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0899110.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IW671_002136"
     CDS             5497..5625
                     /locus_tag="IW671_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0899111.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IW671_002137"
     CDS             5629..6174
                     /locus_tag="IW671_002137"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0899112.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IW671_002138"
     CDS             complement(6628..8319)
                     /locus_tag="IW671_002138"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0899113.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IW671_002139"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IW671_002139"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0899114.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IW671_002140"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IW671_002140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0899115.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IW671_002141"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IW671_002141"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0899116.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="IW671_002142"
     CDS             12601..13302
                     /locus_tag="IW671_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0899117.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2710 c         2368 g         4505 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaat
//
LOCUS       DACEKQ010000082        13130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEKQ010000082 DACEKQ010000000
VERSION     DACEKQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.086"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            162..1103
                     /locus_tag="IW671_002143"
     CDS             162..1103
                     /locus_tag="IW671_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0899118.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="IW671_002144"
     CDS             1172..1903
                     /locus_tag="IW671_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0899119.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="IW671_002145"
     CDS             2047..4227
                     /locus_tag="IW671_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0899120.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="IW671_002146"
     CDS             4247..4729
                     /locus_tag="IW671_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0899121.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="IW671_002147"
     CDS             4745..5506
                     /locus_tag="IW671_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0899122.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="IW671_002148"
     CDS             5637..6971
                     /locus_tag="IW671_002148"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0899123.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="IW671_002149"
     CDS             7278..7763
                     /locus_tag="IW671_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0899124.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="IW671_002150"
     CDS             7756..8439
                     /locus_tag="IW671_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0899125.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="IW671_002151"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="IW671_002151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0899126.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="IW671_002152"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="IW671_002152"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0899127.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="IW671_002153"
     CDS             12055..12684
                     /locus_tag="IW671_002153"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0899128.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="IW671_002154"
     CDS             12736..13068
                     /locus_tag="IW671_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0899129.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4431 a         2321 c         2894 g         3484 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tactatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt
//
LOCUS       DACEKQ010000083        13053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEKQ010000083 DACEKQ010000000
VERSION     DACEKQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.087"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(78..150)
                     /locus_tag="IW671_002155"
     tRNA            complement(78..150)
                     /locus_tag="IW671_002155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IW671_002156"
     CDS             531..1100
                     /locus_tag="IW671_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0899130.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IW671_002157"
     CDS             1087..1947
                     /locus_tag="IW671_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0899131.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IW671_002158"
     CDS             2137..2841
                     /locus_tag="IW671_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899132.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IW671_002159"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IW671_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0899133.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IW671_002160"
     CDS             4678..5994
                     /locus_tag="IW671_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899134.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IW671_002161"
     CDS             5995..6864
                     /locus_tag="IW671_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899135.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IW671_002162"
     CDS             6919..7728
                     /locus_tag="IW671_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0899136.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IW671_002163"
     CDS             complement(7831..8460)
                     /locus_tag="IW671_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0899137.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IW671_002164"
     CDS             8630..9922
                     /locus_tag="IW671_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0899138.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IW671_002165"
     CDS             complement(9998..10660)
                     /locus_tag="IW671_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0899139.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IW671_002166"
     CDS             10885..11169
                     /locus_tag="IW671_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0899140.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IW671_002167"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IW671_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0899141.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4340 a         2255 c         2824 g         3634 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tac
//
LOCUS       DACEKQ010000084        13049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEKQ010000084 DACEKQ010000000
VERSION     DACEKQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.088"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW671_002168"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW671_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0899142.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW671_002169"
     CDS             1873..4539
                     /locus_tag="IW671_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0899143.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW671_002170"
     CDS             4903..5655
                     /locus_tag="IW671_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0899144.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW671_002171"
     CDS             complement(5677..6831)
                     /locus_tag="IW671_002171"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0899145.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="IW671_002172"
     CDS             7016..9457
                     /locus_tag="IW671_002172"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0899146.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="IW671_002173"
     CDS             complement(9787..10491)
                     /locus_tag="IW671_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899147.1"
                     /translation="MKKKQKQELLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="IW671_002174"
     CDS             complement(10678..10923)
                     /locus_tag="IW671_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899148.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="IW671_002175"
     CDS             complement(10940..11791)
                     /locus_tag="IW671_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0899149.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="IW671_002176"
     CDS             11957..12961
                     /locus_tag="IW671_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0899150.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4169 a         2316 c         2716 g         3848 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattc ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaa
//
LOCUS       DACEKQ010000085        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEKQ010000085 DACEKQ010000000
VERSION     DACEKQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.090"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW671_002177"
     CDS             6..305
                     /locus_tag="IW671_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0899151.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW671_002178"
     CDS             378..1739
                     /locus_tag="IW671_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0899152.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW671_002179"
     CDS             1761..2021
                     /locus_tag="IW671_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899153.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW671_002180"
     CDS             2035..2787
                     /locus_tag="IW671_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0899154.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW671_002181"
     CDS             complement(2881..3486)
                     /locus_tag="IW671_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0899155.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW671_002182"
     CDS             complement(3539..4513)
                     /locus_tag="IW671_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0899156.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW671_002183"
     CDS             complement(4891..5670)
                     /locus_tag="IW671_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0899157.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW671_002184"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW671_002184"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0899158.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW671_002185"
     CDS             7161..8558
                     /locus_tag="IW671_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0899159.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW671_002186"
     CDS             8639..9079
                     /locus_tag="IW671_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0899160.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW671_002187"
     CDS             9227..9553
                     /locus_tag="IW671_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0899161.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW671_002188"
     CDS             9554..10303
                     /locus_tag="IW671_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899162.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW671_002189"
     CDS             complement(10383..10838)
                     /locus_tag="IW671_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0899163.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW671_002190"
     CDS             complement(11265..11540)
                     /locus_tag="IW671_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899164.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW671_002191"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW671_002191"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0899165.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEKQ010000086        12597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEKQ010000086 DACEKQ010000000
VERSION     DACEKQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.091"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            556..756
                     /locus_tag="IW671_002192"
     CDS             556..756
                     /locus_tag="IW671_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0899166.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="IW671_002193"
     CDS             1324..1887
                     /locus_tag="IW671_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899167.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="IW671_002194"
     CDS             complement(1948..2292)
                     /locus_tag="IW671_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899168.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="IW671_002195"
     CDS             complement(2389..2634)
                     /locus_tag="IW671_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899169.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="IW671_002196"
     CDS             complement(2704..2958)
                     /locus_tag="IW671_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899170.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="IW671_002197"
     CDS             complement(3122..3445)
                     /locus_tag="IW671_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899171.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="IW671_002198"
     tRNA            3632..3705
                     /locus_tag="IW671_002198"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="IW671_002199"
     CDS             complement(4090..4587)
                     /locus_tag="IW671_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0899172.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="IW671_002200"
     CDS             complement(4627..5436)
                     /locus_tag="IW671_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0899173.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="IW671_002201"
     CDS             5770..6627
                     /locus_tag="IW671_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0899174.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="IW671_002202"
     CDS             6620..7522
                     /locus_tag="IW671_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0899175.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="IW671_002203"
     CDS             7555..7905
                     /locus_tag="IW671_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0899176.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7939..8877
                     /locus_tag="IW671_002204"
     CDS             7939..8877
                     /locus_tag="IW671_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0899177.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="IW671_002205"
     CDS             8955..9848
                     /locus_tag="IW671_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0899178.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="IW671_002206"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="IW671_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0899179.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="IW671_002207"
     CDS             10463..10651
                     /locus_tag="IW671_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0899180.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="IW671_002208"
     CDS             10664..11566
                     /locus_tag="IW671_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0899181.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="IW671_002209"
     CDS             11598..11840
                     /locus_tag="IW671_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0899182.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="IW671_002210"
     CDS             complement(12096..12467)
                     /locus_tag="IW671_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899183.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4461 a         2113 c         2365 g         3658 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataat
//
LOCUS       DACEKQ010000087        12238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEKQ010000087 DACEKQ010000000
VERSION     DACEKQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.092"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            17..166
                     /locus_tag="IW671_002211"
     CDS             17..166
                     /locus_tag="IW671_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899184.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            659..1987
                     /locus_tag="IW671_002212"
     CDS             659..1987
                     /locus_tag="IW671_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0899185.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2189..3022
                     /locus_tag="IW671_002213"
     CDS             2189..3022
                     /locus_tag="IW671_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0899186.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3038..3775
                     /locus_tag="IW671_002214"
     CDS             3038..3775
                     /locus_tag="IW671_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0899187.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3930..4898)
                     /locus_tag="IW671_002215"
     CDS             complement(3930..4898)
                     /locus_tag="IW671_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0899188.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5130..5963
                     /locus_tag="IW671_002216"
     CDS             5130..5963
                     /locus_tag="IW671_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0899189.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5997..6836
                     /locus_tag="IW671_002217"
     CDS             5997..6836
                     /locus_tag="IW671_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0899190.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6862..7422
                     /locus_tag="IW671_002218"
     CDS             6862..7422
                     /locus_tag="IW671_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0899191.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7621..9342
                     /locus_tag="IW671_002219"
     CDS             7621..9342
                     /locus_tag="IW671_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0899192.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9507..10649
                     /locus_tag="IW671_002220"
     CDS             9507..10649
                     /locus_tag="IW671_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0899193.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10665..11876
                     /gene="thiI"
                     /locus_tag="IW671_002221"
     CDS             10665..11876
                     /gene="thiI"
                     /locus_tag="IW671_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0899194.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4164 a         2053 c         2546 g         3475 t
ORIGIN      
        1 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
       61 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
      121 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
      181 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
      241 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
      301 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
      361 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
      421 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
      481 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
      541 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
      601 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
      661 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
      721 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
      781 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
      841 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
      901 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
      961 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     1021 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     1081 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     1141 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     1201 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     1261 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     1321 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     1381 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     1441 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     1501 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     1561 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     1621 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     1681 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     1741 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     1801 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     1861 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     1921 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     1981 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     2041 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     2101 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     2161 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     2221 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     2281 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     2341 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     2401 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     2461 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     2521 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     2581 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     2641 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     2701 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     2761 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     2821 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     2881 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     2941 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     3001 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     3061 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     3121 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     3181 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     3241 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     3301 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     3361 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     3421 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     3481 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     3541 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     3601 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     3661 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     3721 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     3781 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     3841 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     3901 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     3961 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     4021 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     4081 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     4141 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     4201 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     4261 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     4321 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     4381 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     4441 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     4501 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     4561 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     4621 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     4681 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     4741 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     4801 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     4861 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     4921 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     4981 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     5041 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     5101 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     5161 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     5221 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     5281 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     5341 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     5401 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     5461 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     5521 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     5581 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     5641 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     5701 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     5761 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     5821 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     5881 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     5941 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     6001 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     6061 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     6121 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     6181 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     6241 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     6301 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     6361 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     6421 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     6481 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     6541 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     6601 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     6661 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     6721 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     6781 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     6841 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     6901 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     6961 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     7021 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     7081 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     7141 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     7201 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     7261 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     7321 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     7381 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     7441 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     7501 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     7561 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     7621 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     7681 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     7741 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     7801 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     7861 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     7921 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     7981 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     8041 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     8101 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     8161 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     8221 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     8281 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
     8341 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
     8401 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
     8461 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
     8521 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
     8581 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
     8641 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
     8701 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
     8761 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
     8821 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
     8881 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
     8941 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
     9001 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
     9061 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
     9121 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
     9181 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
     9241 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
     9301 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
     9361 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
     9421 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
     9481 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
     9541 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
     9601 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
     9661 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
     9721 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
     9781 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
     9841 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
     9901 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
     9961 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    10021 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    10081 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    10141 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    10201 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    10261 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    10321 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    10381 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    10441 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    10501 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    10561 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    10621 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    10681 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    10741 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    10801 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    10861 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    10921 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    10981 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    11041 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    11101 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    11161 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    11221 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    11281 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    11341 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    11401 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    11461 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    11521 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    11581 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaattattg
    11641 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    11701 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    11761 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    11821 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    11881 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    11941 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    12001 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    12061 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    12121 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    12181 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DACEKQ010000088        11987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEKQ010000088 DACEKQ010000000
VERSION     DACEKQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.093"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(91..453)
                     /locus_tag="IW671_002222"
     CDS             complement(91..453)
                     /locus_tag="IW671_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0899195.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(464..1585)
                     /locus_tag="IW671_002223"
     CDS             complement(464..1585)
                     /locus_tag="IW671_002223"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0899196.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1601..1951)
                     /locus_tag="IW671_002224"
     CDS             complement(1601..1951)
                     /locus_tag="IW671_002224"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0899197.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2081..3592)
                     /locus_tag="IW671_002225"
     CDS             complement(2081..3592)
                     /locus_tag="IW671_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0899198.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3914..5266)
                     /locus_tag="IW671_002226"
     CDS             complement(3914..5266)
                     /locus_tag="IW671_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0899199.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5419..6495)
                     /locus_tag="IW671_002227"
     CDS             complement(5419..6495)
                     /locus_tag="IW671_002227"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0899200.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6691..8436
                     /locus_tag="IW671_002228"
     CDS             6691..8436
                     /locus_tag="IW671_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0899201.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8716..9153)
                     /locus_tag="IW671_002229"
     CDS             complement(8716..9153)
                     /locus_tag="IW671_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0899202.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9137..10510)
                     /locus_tag="IW671_002230"
     CDS             complement(9137..10510)
                     /locus_tag="IW671_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899203.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(10832..11320)
                     /locus_tag="IW671_002231"
     CDS             complement(10832..11320)
                     /locus_tag="IW671_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899204.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(11342..11944)
                     /locus_tag="IW671_002232"
     CDS             complement(11342..11944)
                     /locus_tag="IW671_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0899205.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3441 a         2605 c         2165 g         3776 t
ORIGIN      
        1 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
       61 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
      121 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
      181 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
      241 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
      301 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
      361 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
      421 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
      481 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
      541 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
      601 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
      661 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
      721 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
      781 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
      841 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
      901 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
      961 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
     1021 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
     1081 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
     1141 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
     1201 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
     1261 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
     1321 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
     1381 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
     1441 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
     1501 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
     1561 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
     1621 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
     1681 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
     1741 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
     1801 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
     1861 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
     1921 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
     1981 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
     2041 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
     2101 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
     2161 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
     2221 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
     2281 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
     2341 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
     2401 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
     2461 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
     2521 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
     2581 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
     2641 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
     2701 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
     2761 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
     2821 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
     2881 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
     2941 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
     3001 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
     3061 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
     3121 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacagttcca agtttcaatg
     3181 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
     3241 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
     3301 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
     3361 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
     3421 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
     3481 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
     3541 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
     3601 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
     3661 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
     3721 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
     3781 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
     3841 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
     3901 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
     3961 tgttgctgcc ttttagcata atactatctg ttgctgcaag attttgtttg atagcatcaa
     4021 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
     4081 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
     4141 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
     4201 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
     4261 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
     4321 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
     4381 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
     4441 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
     4501 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
     4561 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
     4621 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
     4681 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
     4741 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
     4801 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
     4861 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
     4921 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
     4981 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
     5041 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
     5101 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
     5161 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
     5221 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
     5281 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
     5341 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
     5401 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
     5461 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
     5521 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
     5581 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
     5641 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
     5701 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
     5761 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
     5821 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
     5881 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
     5941 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
     6001 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
     6061 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
     6121 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
     6181 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
     6241 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
     6301 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
     6361 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
     6421 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
     6481 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
     6541 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
     6601 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
     6661 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
     6721 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
     6781 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
     6841 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
     6901 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
     6961 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
     7021 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
     7081 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
     7141 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
     7201 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
     7261 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
     7321 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
     7381 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
     7441 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
     7501 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
     7561 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
     7621 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
     7681 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
     7741 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
     7801 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
     7861 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
     7921 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
     7981 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
     8041 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
     8101 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
     8161 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
     8221 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
     8281 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
     8341 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
     8401 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
     8461 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
     8521 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
     8581 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
     8641 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
     8701 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
     8761 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
     8821 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
     8881 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
     8941 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
     9001 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
     9061 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
     9121 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
     9181 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
     9241 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
     9301 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
     9361 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
     9421 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
     9481 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
     9541 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
     9601 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
     9661 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
     9721 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
     9781 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
     9841 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
     9901 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
     9961 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    10021 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    10081 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    10141 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    10201 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    10261 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    10321 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    10381 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    10441 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    10501 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    10561 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    10621 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    10681 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    10741 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    10801 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    10861 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    10921 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    10981 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    11041 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    11101 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    11161 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    11221 gtttttgacc aatgatcggc acaaaagctt ccggccatac ttcttgtatg actgccatca
    11281 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    11341 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    11401 ccgaccatgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    11461 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    11521 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    11581 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    11641 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    11701 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    11761 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    11821 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    11881 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    11941 acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DACEKQ010000089        11552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEKQ010000089 DACEKQ010000000
VERSION     DACEKQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.094"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..285
                     /locus_tag="IW671_002233"
     CDS             10..285
                     /locus_tag="IW671_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAQ0899206.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            394..2220
                     /locus_tag="IW671_002234"
     CDS             394..2220
                     /locus_tag="IW671_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899207.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            2233..2949
                     /locus_tag="IW671_002235"
     CDS             2233..2949
                     /locus_tag="IW671_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899208.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            2962..5820
                     /locus_tag="IW671_002236"
     CDS             2962..5820
                     /locus_tag="IW671_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0899209.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            5871..8507
                     /locus_tag="IW671_002237"
     CDS             5871..8507
                     /locus_tag="IW671_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899210.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     RKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDMAND
                     TKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKVENADR
                     IMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELINQKRQE
                     AKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKENLQNS
                     TSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQSSSTSQ
                     PKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVD
                     VRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFT
                     YEDNELFRDTLNDPNPLFDNLN"
     gene            8531..8866
                     /locus_tag="IW671_002238"
     CDS             8531..8866
                     /locus_tag="IW671_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899211.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            8863..9480
                     /locus_tag="IW671_002239"
     CDS             8863..9480
                     /locus_tag="IW671_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899212.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            9496..9963
                     /locus_tag="IW671_002240"
     CDS             9496..9963
                     /locus_tag="IW671_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899213.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            9979..>11552
                     /locus_tag="IW671_002241"
     CDS             9979..>11552
                     /locus_tag="IW671_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAQ0899214.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDEC"
BASE COUNT         4366 a         1498 c         2310 g         3378 t
ORIGIN      
        1 ggaaataaaa tgaaaaattt agttatcagc aagtttgcaa tgttaccaag aatggatttt
       61 ttcgataatg taagtaagtt tatgtcaaat atgacttcaa aactacaagg aatttcacta
      121 tctgtaatta tcttttgtat tgtggttact ggtttgatgt ttcttttcgg agaagggcca
      181 agtcgtactg ctaaaaaatg gttaacttat atcgtgattg gggcaataat tatcttcgga
      241 gcttcaacta tcggacaaac gatcaagtcc gttggtggtt tctaagggga agcttcttag
      301 aactaaaaaa ttcgcaagta ttgcatgaaa gagcattgag tgattaatgc tcttttttat
      361 tttgaaaaac tataaactaa ggagatttaa actatgaacc aacgattcga atataatttt
      421 gaagatgtag aacctaagtt actgaaaaaa ggaaaaggat taaaaaagaa aaataaatat
      481 actctcaaac aagcagaagc attagtagga tggattcaac acaattggtc agacaagaca
      541 acaattacct tgatctgttc agacacagaa gaaggaacaa agtatgcttt tgatggaatt
      601 gaaattactc cattttcgat tggcggacaa gaaaaattat tgtttcctga tattgaagga
      661 caaattttac aagcgccgga cagcatgatt tccgatccta ataaactgat tttggatatt
      721 aacgatcaat acaaacacga accagacgta gcagaaccaa caaaagaatc aaagtcaaaa
      781 ttaaatttga agctcccttc agtagtatta ccatttgcta agaagaaaaa taaacttatg
      841 aacgaattga ttgaaccaga agaaacggat atttatgaag aagaattaga agaacaagaa
      901 gagatcgaag aattagttgt gtcttctgaa gaaacagaag aagtagaaga aattgaaact
      961 gtttctgaaa atgaagaaga aacagaagaa atctttgact ttgaagatga agaagaagtg
     1021 gaaaatgtac agaaaggcga tctatctaca agtagtcaaa atgggttgga agctgtaaat
     1081 gatccacaag ttccaccata tatatcttca caaactccca attactctcc acagtatcaa
     1141 gcgcctaatg tcttacaacc attaagaaaa cacgaaacag tgcagttagt tacgcttaaa
     1201 gagtactgtg atttgggaga agaaattgaa acttcaattt ctcaaatggt cgaaaagttg
     1261 aaaccagaat ctttattcaa attcataggg attccttcta caggtcttac aaatactcgt
     1321 attgatgaat atagaagtaa ccatgctatg ttgcgattag ctgaagtaaa atttgaaaat
     1381 ttaagagagc attattcacg gcaaattatt tcttttaaag cagaagccct agatcgttta
     1441 agaactgcgg tagatattgc ttggaagaag ccttacgatt tagatgtgaa gaaaaacaag
     1501 gccgaagagt tatcaaaaat ggaagcagaa gcagaagcta aattaaatga gttcgtgaaa
     1561 cgtcaggaaa acaatgtaaa tgataaaatc aaaaagtttg agatcgaaca agaaatagat
     1621 ttaaaaaact ttattgcaag acaagaggca gataaagcgg tatttattgc tacagagaaa
     1681 gatcggactc aaaatttaat tgattctaaa gcggaatcaa tcaatgcaga tttacttgaa
     1741 acaaaagata aatttattga tgaagaaatg tacaaaatga aaattgaaat gaacaaaaat
     1801 ttatttgatg gcaagcaaaa aattaaatca gaattggcta ctgaactcgc taaggcaaat
     1861 gataatgttt gggaaaatgc gctgaaattt attcaagaaa ttcaagatga aattgaagct
     1921 aaaacaccaa tttgggctgc tgaaatcaag gagtttaata gtttagaaga acaagaacat
     1981 gaacgtatga agcaaactaa agaacttcaa ttaaaagagg aaagtattga aatcaagcgt
     2041 ttggaagctg aaatgaattt gaagcgtatg gaagaattag atcatgaaaa taaaatgctt
     2101 aaaattaaac ttgaaactgc cctttcaaaa aatgagttat tagctatgga acaagataca
     2161 aagcaagtgg tgaatggatc aaacagagtt ggtgcactta atttatttag taaacgttaa
     2221 aggagaacgg atatgagtgt ttttaataat aaagttatgt tagggaactg ttatgtgaac
     2281 cacattggtt tttacgatgg aggaacatac agagttttat tcttacgttt atctgttttt
     2341 agttactcaa aagatggaaa ggggcattat gaaaatatta atgcacaaat ttcttgtcct
     2401 ttagatagaa atggggaaac cacgactaca agtagtcacc ctaaagcctt gatgaataaa
     2461 tttatctttg aaaagttgga tcgcaatttg gttgagggga tgaagataag tattcatgga
     2521 aatattcgtg gaacagaaca agtgcttctt aacagtggtt ggaaaaactt tggtcaatta
     2581 tcgcctttag aaaaagaaga atatcaaaaa attcctaaaa atgaattagt aacaagaaat
     2641 caaacatata tctatataga agaggtaagt atgccagaaa aagaagaatt tagaaagtct
     2701 caatctaagg taacaaaaga agatgtggaa gttgcagaac cgaatcaagc agatgaaatt
     2761 ttatccgatg atactgatcc agtgaatgat gaaggagagg ttattgaagt ttttgaagct
     2821 gaaattatcg aagaggaaga agaaattaaa agttttgata aaagaatgta tcgagagaaa
     2881 ccaaaaggtt atgtgaattt tgaagatatg tattatgacg aatattctaa tgaaccaccg
     2941 atcaattagt gggggtcaaa tatgaagcta gtacaaaaca atgaagtgaa agaaataaaa
     3001 aacggctttg ttaaaaactc gaacaaaatt ttgggaaagc tgaaaaaagt aggtagtttc
     3061 aaaaaaaata aatatcatac tggaaaaatt gagggagaag atttaccaaa aagtttacaa
     3121 gataaattga atgttccgct tgtatttata ggtgtttttt taggttcaat agtttttcta
     3181 gcaataacgt acttattgaa ctttttaaaa gctttgttag ttgcaatcgg acagtcagaa
     3241 agtgggctac ttggtgtaga gttcgatcct aagtttagtt tggcatatct aaaaccactt
     3301 ggggatacga aacaatactt tatgtgtgct gtggttgcgg taataggagt cgcttattta
     3361 atatatggga aattaaatca tgaaagtgag aaaaatcttg tttacggtca aaaaggagat
     3421 tcaagacttt caactatcaa agaaattaag agagattacc atgagatacc agaaaaaaca
     3481 gaagtgtatg atggtattgg tggtgtgcca attagtcact atcaagataa atattatatt
     3541 gatagagaca cagtaaatac cgttgttttg ggagcttcac gatccggtaa aggggaaaca
     3601 tttgttgttc ctttattaga taatcttagc cgagcaaaaa tacaaagtaa tatggtcgtg
     3661 aatgatccga aaggagaatt gtttagcgct tcaaaggaaa cacttgaaaa acgaggttat
     3721 cgtgtattag tgctaaatat tgatgatcca cttgaatcaa tgagttttaa tcctttacaa
     3781 ctagtaattg atagttgggc taacaaggac tatcacgaag ctagtaagcg agcgaatact
     3841 ttaacttcaa tgctctttgc ttcaggaatg ggtacagata atgagttttt ctataaatca
     3901 gctaaatcag cggtcaatgc gattatttta acgattgtag aacactgttt taacaatgat
     3961 tgcattgaaa aaattacaat gtacaacgtg gctcaaatgc tgaacgaatt aggaagctta
     4021 ttttacactg atccaaagac tggaaaagaa aaaaatgctt tagatgaata tttcaatact
     4081 ttaccacaag ggaatcaggc caagattcag tatggttcta cttcatttgc cggtgataaa
     4141 gccaaaggtt cgattttatc tactgcttca caaggtattg aaatgtttac aagtgatttg
     4201 ttcgggaagt taacaagtaa aatgtctatt gatttgaaag agataggctt tcctaagtcc
     4261 atacaattta aagtcaatac gaatttagtt ggaaagagga ttagtatttc atttttgaga
     4321 aaagaaaatg atgtaattag attaattaag aagtatcgag tgaaagtaaa agctttaggg
     4381 ctatgtgttt taaattttaa tgaatatctt caggatggag atatgttgga tatacgatat
     4441 gaagaagaga aatcgagagc atggtatcgt attgaatttc ccaaaaaaca agcaaatagt
     4501 catgatactt ctttagtcac taaaaaattc aagtcaggta aaaattattc tgacttagaa
     4561 atcagcacct tacgattaaa atacacggat cagccaactg ctgtgttcat ggttattcca
     4621 gattatgatc cgtcaaatca cgtacttgca agtattttta tcagtcaact ttatacagaa
     4681 ttagcaagta attgtaagca aacaccggat aaaaaatgtt ttaggcgagt tcatttcttg
     4741 ttagatgaat ttgggaatat gccggctata gacaatatgg atgggattat gacggtatgt
     4801 ctaggaagaa atatgctttt tgacttagtg attcaatcct atagtcagct agaaacaaga
     4861 tatgataaag cttttaaaac aattaaagaa aattgtcaga atcatatttt aatcatgtcg
     4921 aacgatgagg aaacgataga agagatttca aaaaaatgtg gtcataagac aacaataaat
     4981 agaagtgcta gttctaaaca tttagatact gatagctcgg tcactagttc ggcagagcaa
     5041 gagcgagtaa ttacagttga gcgtgctagt caattgattg aaggggaaca aattatttta
     5101 agaaaccttc atagacaaga tacaaaaaga aataagatta gaccatttcc tatttttaat
     5161 actaaagaaa caaatatgcc gtatagatac caatttttag cagaagactt tgatactcaa
     5221 agagatatta atgagattga tattgcatgt gaacatatca atctatcttt acaagataac
     5281 caaattccat atgcaaggtt tatcaaagat ttaaaaacac gtttggagta ttcaattatt
     5341 aataatattc ctattagtga agaagactat caggattatc taaatttatc aggatcacaa
     5401 caaacaatta atgaagaaga gctgttaaaa ctggtaaata cggaagtaaa aagtcaagat
     5461 atggaaggtc aagttttatc agaacaagaa atgaaaatta atatttatct aaaggaaagt
     5521 caaaaggaag caatagaatt agtaaaagta attaatggaa ggttagctga tgaaaattca
     5581 gcagatatgg ttataaaaac cattaattca caaatccttg atacgccttt cattcaatct
     5641 ttaatcaatg attctatagc tattcataat gttcaagaga gtaaggatca aattatgatc
     5701 ctagaacaac aattagctaa tgcttatgag ttagcagtgt ttaaaacgaa aaattcaata
     5761 ctgaaaaata aaattatcaa gttgaaaaat gctatgacta agctagcaaa agcaatttag
     5821 agagaaggga agaaatattg actgaatcta ttaattggag aaaactaaat gtgcaatggt
     5881 taaagtataa atggccaatc atagtttccc taataatcat tctatttatg gcctatccgc
     5941 aacgagtttt tgcgttgccg tggattacag acaataaaga ggtattaaaa tttttaacaa
     6001 aatatcaaga ttatcttcag tttactaaag gtggttttgg aagcattgtc gttcaaagtt
     6061 tatcgtgggg gctgattaga ggtgcatatt ttgtttgtaa atcagcagaa aaccttgttc
     6121 cacaatcgct aagtttgttt agctttgttg aaagcagtag tttaggtgct gtttatcaat
     6181 ctgtattaaa cacggtggtt gtaggattaa tgatcctttc attgatgttt gttggttaca
     6241 agatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt
     6301 ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa tttgctaaaa
     6361 cattttataa ggactctact acgattacaa aagctgatgg gggcgttgct tggtcattga
     6421 ttgaggacgg tgtaactgat ttagtatata tcaataaaaa cgatgcttat gatcggttgg
     6481 gtaaagatgg cgtacaaaaa aatgcgttaa aagaaaagaa tttcttttcc tacgatttca
     6541 gtgaagtaat cacagttgat acagctaaag atttagaaga tgataataaa aaagctgaat
     6601 atcttcaatc taggttgaca ccagatggcg aaggaaatat gcaagccgtt aagattgatg
     6661 atggttggat gtcgatgttc tctgatagtt tcaaaggagg gtactacagg tttactaaaa
     6721 atacttttgc attgacttta ggattacttt cgttagctgt agcctatgtt tttagtattt
     6781 ttatcatagt caccgctatt ttagaactgg catttaagcg tgtgttaggg gttcttgttt
     6841 ttgcaacaga tattgagaca ggacagcgtt caaaagttgt tctatcagat attctacagt
     6901 gctatttgac tgtaggcttt caaggatttg gattatccat gtttgcaatg tttgtttcct
     6961 atttaggaac aggcggaggg tcttccgtta atccgtttat taaggctgta gcttatgtat
     7021 gtgccgtttt tgttttaatc aaaggcagtg gaacagtcat gagatacttt ggtgttgata
     7081 ttggactcaa agaaggctat gggcaacttg cttctgcttt tggaatgggt gcaatgctgt
     7141 ttagaaaagg atcaaatgga tttaatcgtg ccaaaggaag cggaaacgga aatggatcag
     7201 acagcggaga aggagaagat cgaaaaccag aaaagaattt cggtgaaact ctatcaaaaa
     7261 aagctggcaa aactggaaga gctttgggat atgcccatga acgtggatta tcaggtttag
     7321 cttcagatgg agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg
     7381 atatggctaa tgatactaag aataagttca aagaaggttt agatgatggt acagtgtccg
     7441 ctattaataa aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat
     7501 acgctgattc catgccaaca cgtctttcgg atagaaaaga tggtgctaag gttgaaaatg
     7561 ccgatagaat tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat
     7621 cgaataataa tcctgtaagt gcaattcagc aaaaagttca acaagatatt gaagaacgta
     7681 aaaatgctat gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgacaag
     7741 aagctaaata cacgcctgaa gcaatgaatc gtgaggaaat gttaaggaaa cgtgtagaag
     7801 ggcgtacagg aactaatacg gacgaaaaag aagcgttgac caaagaaaga atccaagaag
     7861 ctaaaaactc aaataccgga ttagaaaaat tggtcaaaga aaatctacaa aattctacaa
     7921 gtagtcaagg tggtcgatca gtagatgtcc gagaaaatat tcaaggttca ttgaatggtc
     7981 atgtcggacg gaatgttgat gtaagagaga atttacaaag ttcttcaaat ggtcaaggta
     8041 ctaagacaat tggtgttcga gaaaatctgc aaagtagcag tacaagtcaa ccaaaaacag
     8101 tagatgttcg agaaaatgta caaaattcta caagtagtca agggtttaaa tcagtggatg
     8161 ttcgagagaa tgtgcaaggt agcagtgcaa gtcaaccaaa aacagtagat gtccgagaaa
     8221 atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc
     8281 aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa
     8341 agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg
     8401 aaaatgataa gggatcaaaa ccaagacgaa gattcactta tgaagacaac gaattattca
     8461 gagatacact taatgatcct aatccacttt tcgataattt aaattaaatt aaaagccggc
     8521 atgagtgatt atgccggctt tttatgaggt gaaaatgatg tatagaaatc ccaaaaatat
     8581 aaaacaagag ataaagctat ttttcttcta tttagtcgat ttaggaatag tagccggaag
     8641 cgtgatggca agtgtttact ttgcaaaatt aatgccaatt tcaccaatga tgaaatttgc
     8701 actgtactta ttagcatttg tagtgggtgt ttggatgtgc gttagaacgc ctaatcaccc
     8761 taaagagcga aatattttaa tgctgtatta tctctttaaa atggatagaa acagatacca
     8821 cgaagtagag tttaaaagaa atagtagggg gaaaattgaa ttatgattgt tgctgaaagt
     8881 aatgaaaata aattaagtgg tgtttttgac aaattcttag aactagttat tccaaaagat
     8941 aaagatatgt gtgaactttt accatataaa aaaatattga aagacggaac gttagtgact
     9001 aatgacaata agtatcaagc cttttttaaa gttaaaacaa ctgacttaac ttctatttca
     9061 gaagatggat ttatgcgtat ggtaaacaaa ctcacttatt taaatcgagt atatttagaa
     9121 ccatataaaa tttattcatt aacttatcct actgaaacag taaatcaagc gatttattgg
     9181 aagcatagaa tacaggaggc aaagagaagt ttattgagta ataatgatcc caatttagaa
     9241 acgtatctga agtggaatct aaagctagct tacgataatt atgaaagagt tgatctagta
     9301 tcgactttac cagagttgat gttcgttatt tgtgtttatg gaaaaacaca aaaagaagtg
     9361 aaaaatcatg ccgatgtaat gaaacaggcc ggtggtagag aattagattt acaaagaatt
     9421 actgaagggg atttaatcaa tgtgataact aaattgatga atcccaatac ttctttataa
     9481 ggcggtgctt attatttgtt aaagcactgt agaaaaggat tttgtaaagt aattaaaggt
     9541 aaaaatgctg ctgatttaat ctttgaatgg aaagaatcta atttaattaa tgtacgtggg
     9601 aaaaatggaa gcggaaagac agaaagagtg atggctagta ttgataattc aaggcctaga
     9661 tcaagagcat tagtaataac tgatgaaaaa aatcactata aaggcatacg aacgatagaa
     9721 aaaaaatatt taactaacaa tgatttaaaa gtagtagatg atattttaaa tatttatcct
     9781 gattttaatt tagttgtttt tgatgaaaca attcctgtga cagaaataaa aggattctat
     9841 caaagctttt ccaatctagc aaataaatat gaggaagttc tgtttgtttg gattacagaa
     9901 gaagatagta atagcaagtc agcgttcttt agtgaaaaga acgctttttt acattctatt
     9961 taaaaaggag aattgaacat gattgtaagt aaaaaactag caagaaatct agtaagtgaa
    10021 gggtatgact taagattagt agaaaagtat caaccgagcg gtggaattaa atttgatgag
    10081 cgttatattt ctactggtga tggatatgta acttcgattg ctgttattga gtttgcagaa
    10141 gatgtaaata gtcgatggtt aacggatata atggcaattc caaatactat tgcaactttc
    10201 gatgtatgta cggcggataa agaagactta ttacacaaaa tcaatacgtc tattggagaa
    10261 ttagattacc gatcaacaaa tgagaataga acaactgaca aaacagacgc tttactagat
    10321 aaatacaatt tagaagattt tgcacaagaa attacgaaac gtggtgaagt tgtcaaaact
    10381 gttcatgctc gtatttttgt ttatagcgat tctttagaag aattagagaa aagaattagt
    10441 gagattcgta aagatttggc tagtgtcaat cacaaggcaa ttgttccgca atttagaatg
    10501 aaggatgaat ggatgtcttt atttcaagat ggcatggaac aagaatcaaa attatttgcc
    10561 aagaaaggct taactgttcc ggcaaaaaat attggtggcg gtattccatt tcaccacaag
    10621 agtttgaaag atccacgtgg gttttattta ggaactacgt caactggtgg tgcttttata
    10681 tttgatccat tttattctac gaagcaaaga cggtcgttta acggctttat tttaggaaag
    10741 atgggttttg gtaagtcaac tttaattaaa cagctacagg aaggcctaca agcgaaaaat
    10801 tgttttattc gaggatttga caagtcaggt gaatttgaag gcatggtgaa gaaagcaggc
    10861 ggtgtatacg tcgatttagg cggtggtgat atggtagtaa atccattgca aatttatgca
    10921 acagtcatga aaaaagatca atttggaaat gatgttgtag atgaatcaaa gagttttcaa
    10981 aatcatatca ctaagtgtag taatcaaatt cgctttttag aacctgaaat tgatacggca
    11041 actgtacgtg aatatacttt gatgttacaa gacttttata ttcattatgg aattttgcca
    11101 aaagaatgga gagaagcgag agacgacaac gaacgaaatc aattaaatac aaatatttgt
    11161 aacttgccta atgattcata tccaatctat ggagagtttc ttaaatttat tgaaaatcaa
    11221 caattcaaaa ttaaaaatgc tactcctgaa cgtgtccgtt ctattgagct aattaagctt
    11281 atcgttacgg atatgtgtac aacttatccg caaattttca acggagtaac aacaatgcca
    11341 aactttgata caacaaaaat tgtttactat aacattgatt ctattagtaa aatgagtgcc
    11401 ccagtattcc attgccaatt atttacagcg ttaacgacta tttggaacag tgccttgcaa
    11461 aacggaaaga ttcaaaaaga aaactttaaa aaatatggca gtgattatta taagtatgca
    11521 caagatttca tggttttgat cgatgagtgt ca
//
LOCUS       DACEKQ010000090        11510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEKQ010000090 DACEKQ010000000
VERSION     DACEKQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.095"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..2154)
                     /locus_tag="IW671_002242"
     CDS             complement(79..2154)
                     /locus_tag="IW671_002242"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0899215.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IW671_002243"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IW671_002243"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0899216.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IW671_002244"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IW671_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0899217.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IW671_002245"
     CDS             complement(4412..5311)
                     /locus_tag="IW671_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0899218.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IW671_002246"
     CDS             complement(5326..5727)
                     /locus_tag="IW671_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0899219.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IW671_002247"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IW671_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0899220.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IW671_002248"
     CDS             complement(6198..8390)
                     /locus_tag="IW671_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0899221.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IW671_002249"
     CDS             complement(8414..9385)
                     /locus_tag="IW671_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0899222.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IW671_002250"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IW671_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0899223.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2419 c         2105 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc gtgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
//
LOCUS       DACEKQ010000091        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEKQ010000091 DACEKQ010000000
VERSION     DACEKQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.096"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW671_002251"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW671_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0899224.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW671_002252"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW671_002252"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0899225.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW671_002253"
     CDS             complement(2169..2906)
                     /locus_tag="IW671_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0899226.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAENPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW671_002254"
     CDS             complement(2997..3524)
                     /locus_tag="IW671_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0899227.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW671_002255"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW671_002255"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0899228.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW671_002256"
     CDS             complement(4401..4886)
                     /locus_tag="IW671_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0899229.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW671_002257"
     CDS             complement(5039..5236)
                     /locus_tag="IW671_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899230.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW671_002258"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW671_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0899231.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW671_002259"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW671_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0899232.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW671_002260"
     CDS             9211..9423
                     /locus_tag="IW671_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0899233.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW671_002261"
     CDS             9425..11104
                     /locus_tag="IW671_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0899234.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1970 g         3590 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa gggttttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACEKQ010000092        11128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEKQ010000092 DACEKQ010000000
VERSION     DACEKQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.097"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="IW671_002262"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="IW671_002262"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0899235.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="IW671_002263"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="IW671_002263"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0899236.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /locus_tag="IW671_002264"
     CDS             2139..3020
                     /locus_tag="IW671_002264"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0899237.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /locus_tag="IW671_002265"
     CDS             3017..3733
                     /locus_tag="IW671_002265"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0899238.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="IW671_002266"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="IW671_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0899239.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="IW671_002267"
     CDS             4450..5451
                     /locus_tag="IW671_002267"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0899240.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="IW671_002268"
     CDS             5448..6809
                     /locus_tag="IW671_002268"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0899241.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="IW671_002269"
     CDS             6769..7920
                     /locus_tag="IW671_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0899242.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="IW671_002270"
     CDS             7895..9064
                     /locus_tag="IW671_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0899243.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="IW671_002271"
     CDS             9239..10618
                     /locus_tag="IW671_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0899244.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="IW671_002272"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="IW671_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3703 a         1945 c         2493 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgagaaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DACEKQ010000093        10565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEKQ010000093 DACEKQ010000000
VERSION     DACEKQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.098"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..505
                     /locus_tag="IW671_002273"
     CDS             <1..505
                     /locus_tag="IW671_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899245.1"
                     /translation="IQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFS
                     GKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMM
                     FEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
     gene            492..1913
                     /locus_tag="IW671_002274"
     CDS             492..1913
                     /locus_tag="IW671_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0899246.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1926..2933
                     /locus_tag="IW671_002275"
     CDS             1926..2933
                     /locus_tag="IW671_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0899247.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2945..3949
                     /gene="neuB"
                     /locus_tag="IW671_002276"
     CDS             2945..3949
                     /gene="neuB"
                     /locus_tag="IW671_002276"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0899248.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3946..5094
                     /gene="neuC"
                     /locus_tag="IW671_002277"
     CDS             3946..5094
                     /gene="neuC"
                     /locus_tag="IW671_002277"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0899249.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5067..5744
                     /locus_tag="IW671_002278"
     CDS             5067..5744
                     /locus_tag="IW671_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0899250.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5734..6876
                     /locus_tag="IW671_002279"
     CDS             5734..6876
                     /locus_tag="IW671_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0899251.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            6889..7866
                     /locus_tag="IW671_002280"
     CDS             6889..7866
                     /locus_tag="IW671_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0899252.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            7866..8774
                     /locus_tag="IW671_002281"
     CDS             7866..8774
                     /locus_tag="IW671_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0899253.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            8775..9527
                     /locus_tag="IW671_002282"
     CDS             8775..9527
                     /locus_tag="IW671_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0899254.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            9532..10479
                     /locus_tag="IW671_002283"
     CDS             9532..10479
                     /locus_tag="IW671_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0899255.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3810 a         1340 c         1955 g         3460 t
ORIGIN      
        1 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
       61 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      121 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      181 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      241 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      301 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      361 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      421 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      481 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      541 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      601 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      661 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      721 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
      781 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
      841 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
      901 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
      961 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
     1021 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
     1081 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
     1141 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
     1201 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
     1261 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
     1321 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
     1381 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
     1441 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
     1501 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
     1561 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
     1621 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
     1681 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg
     1741 acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg atacttcata
     1801 ttttaatcaa tcaaatgttg attcgttacg ttttgacatc attattgata attagtttag
     1861 ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca taggaggaga
     1921 tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc attggaagtg
     1981 aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat agtagaggtg
     2041 aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg agatttttaa
     2101 ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg gatattattt
     2161 ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg gaagctgtaa
     2221 aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac
     2281 attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg ggtgcgacaa
     2341 agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa acagaaacaa
     2401 tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt attcctcttt
     2461 ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat atgacacgat
     2521 ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg aattcaaaat
     2581 ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta gctcaatcat
     2641 taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata aaagttgtag
     2701 gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa attgaacgct
     2761 catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact gacgaactgt
     2821 acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca gaattaatga
     2881 gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg taaggagttt
     2941 tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata atggaagtct
     3001 agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg cggttaaatt
     3061 tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa aatatcagaa
     3121 ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag aattatctca
     3181 atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt ttttgtctac
     3241 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
     3301 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
     3361 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
     3421 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
     3481 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
     3541 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
     3601 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
     3661 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
     3721 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
     3781 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
     3841 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
     3901 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
     3961 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
     4021 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
     4081 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
     4141 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
     4201 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
     4261 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
     4321 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
     4381 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
     4441 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
     4501 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
     4561 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
     4621 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
     4681 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
     4741 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
     4801 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
     4861 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
     4921 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
     4981 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
     5041 atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta ttaaaatata
     5101 ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt attgaaacct
     5161 taagattaga aaataagtta atatatggat tttttgatga taagcaaaat aaaatgcaag
     5221 atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa
     5281 tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat
     5341 tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag
     5401 aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg
     5461 taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta
     5521 ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag
     5581 gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta
     5641 tgataggcgc agggagcact attgttaagg atattccctc acattcctta gcttatggca
     5701 atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt
     5761 cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata
     5821 gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct
     5881 aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg
     5941 cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat agctacagta
     6001 aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc taatacatgg
     6061 aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa caaccaatta
     6121 cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga acgaatttta
     6181 gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc gttaggttct
     6241 acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc atttaacggt
     6301 aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa aaaatatgtt
     6361 gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta tcaacatagc
     6421 gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg tggacaaatg
     6481 gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata taaggaagct
     6541 ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt
     6601 tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt taacgtaata
     6661 aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact acatttgcaa
     6721 ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt gtcagaaaag
     6781 ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag tgaacaagaa
     6841 tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat gaacatagga
     6901 attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga acactttaat
     6961 gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa
     7021 tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga aataaattca
     7081 gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc tttaaattta
     7141 gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga aataatcgaa
     7201 gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa gagaaaagaa
     7261 attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca ataccatgta
     7321 ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt tgtttcaaac
     7381 aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat tatagataca
     7441 tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct tgatattgat
     7501 tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg aactttaagt
     7561 gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga aaagatttta
     7621 atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac ttctagtatg
     7681 acagatattc aattgtacga tgaggttatg catgataata attattcaca tactattatt
     7741 gaagatgcat atttagagga gattaaagaa tttttcacgc ttttggaaaa gaaaatggaa
     7801 ccccaatata cttttgaaaa agataagtat attattaact taattaacga cattgaggta
     7861 aattaatgat gaaagtaatt ttttttgggt tagggtcaat tggaaagaga catctaaaaa
     7921 atctaataag ctatggaaaa aagaattcta ttaattttga tataacagct tatagtagca
     7981 gtggaaaaaa agagtttgaa gaaataacgt atattcaaaa tagagacgat ttggatacac
     8041 attatgatat agcttttatt acaaatccga cttttaagca tctggaaaca ctaaatgtaa
     8101 ttgttgaaaa agttgattac atatttttag aaaaacctgc tttttctgag agtattgatc
     8161 ttactcaata taattatgat tacaaaaata tttatgttgc tgctccactg aggtataaaa
     8221 atatcatgga agttgttcgt aatagtattc aaagtaaaaa agtatattct gtaagagcta
     8281 tttgttctac ttatcttcct gattggagaa aaggtactga ttatagaaat aattattctg
     8341 cctttaaatc tatgggggga ggagtagaat tagactgtat acatgaattg gattatatta
     8401 taaacctatt tggtttccct aaatcagtaa aaaaacagtt tggtaaatat tcgaatcttg
     8461 aaattaacag taatgattta gcaacatata tattgagata cgatgataaa attatagaaa
     8521 ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt actttagatg
     8581 aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac attgttaaag
     8641 aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc tttgaaaacg
     8701 tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta ttgaaaatag
     8761 ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg tctaaggggt
     8821 ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc gctttagcaa
     8881 tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt ttgaatacgg
     8941 acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa attgtagaac
     9001 gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt gattcattct
     9061 tacaactaga aagagaagat aatatatttg atttaattat agatttagat ttgacttcac
     9121 ctttacgtga agtttctaat cttgaagaat taatagatga acatctaaaa aatttagata
     9181 aagacgtcta cttttcagtt gtttctgcaa gacgtaatcc ctattttaat atggtaaaaa
     9241 gaattgaaaa tgaagttact atagttaata aatcaagata cacatctagg caacagacac
     9301 ctgaagtttt tgaaatgaat gcctcaatgt attcttttaa acctgaattt ctaaaaaaaa
     9361 gtaattatat ttttgatggg caatgtggga taataaaaat gagagattat ctaattttag
     9421 atattgatag tgaagaagat tttaaatggt tagaattttt gtttcctaaa tttttagaag
     9481 attcttcgga gcttcaagat atctataatt atttatatcg gaggtaaaag gatgagctgt
     9541 tatcttatca ccggtggggc tggcttcatt ggttctacct tagccaatca tttaggagaa
     9601 gaacatgtgg taatcgtcgt cgatgactta tcaatgggaa aagtagaaaa tcttgatatg
     9661 acgaaaaata tcacttttat tgagggagat gtcgctgaga aatccttgat ggaagagatc
     9721 atgaaagcta ataaatttga ttatatcttt catttagcag cagttgctag tgtggcagat
     9781 tcagttgccc gaccagtaga aacacatcgt gtgaattttg aaagtgtatt aatgatttta
     9841 gaattgatcc gcaagtatca acctgattta aaacggatcg tgtttagttc ttcagcggct
     9901 gtttatgggg atgaaccgac tttaccaaaa aaagaagagt ccgtgattcg cccattgacg
     9961 ccttatgcgg ttgataaatt cgctgcagaa caatatgtgc tagattattg tcatttatat
    10021 gatgtacccg gtagcgcagt tcgttttttc aatgtctatg gtccaaatca aaatccgaat
    10081 tctccataca gtggtgtgat ttctattctc gtagatcgct ataagaaaca aatcgtagga
    10141 gaagcaactt catttacatt atttggtgat ggaagccagt ctagagactt tgtttatata
    10201 gatgatgtaa tccaagcatt acttttagtt gcgaatgagg aagctgcact ggggaaacaa
    10261 ttcaatgttg gaacgggtaa agcaaccaca cttttggaat tgattcatgc aatcaatgaa
    10321 attctcggtg tagaattgac acttgaatac aaagaagagc gttcgggtga tatccatgac
    10381 tctctagcag atattactaa gatccagtca attggctatc aaccgaaata tggtatttta
    10441 agtggaatga ctaattattt gaaaactgaa atcaattaaa acatactaag ataagattag
    10501 agggtaaatt caggctcttt gtcaataagg actgatgagt tttgtaaaat caaatctggg
    10561 ataga
//
LOCUS       DACEKQ010000094        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEKQ010000094 DACEKQ010000000
VERSION     DACEKQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.099"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IW671_002284"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IW671_002284"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0899256.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IW671_002285"
     CDS             1007..1927
                     /locus_tag="IW671_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0899257.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IW671_002286"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IW671_002286"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0899258.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IW671_002287"
     CDS             3233..4711
                     /locus_tag="IW671_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0899259.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IW671_002288"
     CDS             4988..7048
                     /locus_tag="IW671_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899260.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IW671_002289"
     CDS             7131..7700
                     /locus_tag="IW671_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899261.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IW671_002290"
     CDS             complement(7875..8681)
                     /locus_tag="IW671_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899262.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IW671_002291"
     CDS             8819..10333
                     /locus_tag="IW671_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0899263.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3702 a         1739 c         2055 g         2936 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACEKQ010000095        10155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEKQ010000095 DACEKQ010000000
VERSION     DACEKQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.100"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..1257
                     /locus_tag="IW671_002292"
                     /pseudo
     CDS             1..1257
                     /locus_tag="IW671_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1364..2395)
                     /locus_tag="IW671_002293"
     CDS             complement(1364..2395)
                     /locus_tag="IW671_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899264.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(2402..3232)
                     /locus_tag="IW671_002294"
     CDS             complement(2402..3232)
                     /locus_tag="IW671_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899265.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(3229..4035)
                     /locus_tag="IW671_002295"
     CDS             complement(3229..4035)
                     /locus_tag="IW671_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0899266.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(4041..4865)
                     /locus_tag="IW671_002296"
     CDS             complement(4041..4865)
                     /locus_tag="IW671_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0899267.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(4855..5955)
                     /locus_tag="IW671_002297"
     CDS             complement(4855..5955)
                     /locus_tag="IW671_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0899268.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6133..6918
                     /locus_tag="IW671_002298"
     CDS             6133..6918
                     /locus_tag="IW671_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0899269.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6951..7334
                     /locus_tag="IW671_002299"
     CDS             6951..7334
                     /locus_tag="IW671_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0899270.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            7410..7541
                     /locus_tag="IW671_002300"
     CDS             7410..7541
                     /locus_tag="IW671_002300"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0899271.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            7510..7746
                     /locus_tag="IW671_002301"
     CDS             7510..7746
                     /locus_tag="IW671_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0899272.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7824..8795)
                     /locus_tag="IW671_002302"
     CDS             complement(7824..8795)
                     /locus_tag="IW671_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0899273.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            9429..>10155
                     /locus_tag="IW671_002303"
     CDS             9429..>10155
                     /locus_tag="IW671_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0899274.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDN"
BASE COUNT         3030 a         2299 c         1966 g         2860 t
ORIGIN      
        1 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
       61 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
      121 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
      181 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
      241 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
      301 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
      361 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
      421 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
      481 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
      541 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
      601 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
      661 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
      721 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
      781 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
      841 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
      901 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
      961 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     1021 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     1081 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     1141 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     1201 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     1261 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     1321 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     1381 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     1441 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     1501 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     1561 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     1621 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     1681 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     1741 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     1801 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     1861 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     1921 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     1981 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     2041 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     2101 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     2161 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     2221 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     2281 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     2341 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     2401 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     2461 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     2521 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     2581 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     2641 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     2701 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     2761 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     2821 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     2881 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     2941 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     3001 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     3061 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     3121 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     3181 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     3241 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     3301 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     3361 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     3421 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     3481 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     3541 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     3601 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     3661 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     3721 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     3781 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     3841 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
     3901 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
     3961 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
     4021 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
     4081 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
     4141 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
     4201 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
     4261 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
     4321 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
     4381 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
     4441 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
     4501 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
     4561 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
     4621 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
     4681 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
     4741 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
     4801 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
     4861 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
     4921 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
     4981 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
     5041 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
     5101 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
     5161 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
     5221 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
     5281 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
     5341 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
     5401 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
     5461 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
     5521 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
     5581 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
     5641 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
     5701 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
     5761 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
     5821 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
     5881 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
     5941 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
     6001 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
     6061 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
     6121 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
     6181 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
     6241 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
     6301 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
     6361 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
     6421 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
     6481 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
     6541 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
     6601 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
     6661 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
     6721 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
     6781 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
     6841 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
     6901 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
     6961 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
     7021 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
     7081 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
     7141 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
     7201 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
     7261 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
     7321 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
     7381 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
     7441 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
     7501 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
     7561 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
     7621 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
     7681 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
     7741 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
     7801 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
     7861 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
     7921 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
     7981 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
     8041 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
     8101 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
     8161 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
     8221 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
     8281 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
     8341 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
     8401 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
     8461 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
     8521 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
     8581 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
     8641 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
     8701 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
     8761 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
     8821 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
     8881 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
     8941 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
     9001 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
     9061 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
     9121 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
     9181 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
     9241 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
     9301 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg ttatccagac
     9361 tgtagacaaa gtccatttta tggatgatgt ctacagtctt ttcttttata atagatttat
     9421 gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat ggtagaaatc
     9481 aatttgcgat gcttacgatt gatgatctag tttctcaaga tcatctcgtt cgaaagattg
     9541 atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac tccgatttag
     9601 gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag tatttattcg
     9661 gtatccgttc catgcgacaa accattaagg aagtcgatac caatgtcgct taccgctggt
     9721 ttttaggcta ctccttcgag gagaaaatcc cccatttctc aacttttgga aagaattatg
     9781 ttcgccaatt tagagaaacg acggtgtttg aggatatttt tgcctatatt ttagaacaag
     9841 cagttaaagc aggttttgta acagaggata atttgtatct ggattctacc cacatcaaag
     9901 ctaacgccaa taaacataag tttacgaaag aaatgaccca tgatgaagcg aaagcctatc
     9961 aggatgaatt agaagatgag atcaatcgtc aacgaataga ggctggaaaa cgccctttta
    10021 ctttggactt agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa gctgatccgg
    10081 aaagcggcta ctacgtcaaa ggcgaacgag aaaaacaatt tgcttattct gtccatactt
    10141 catgcgacga caatg
//
LOCUS       DACEKQ010000096         9809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEKQ010000096 DACEKQ010000000
VERSION     DACEKQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.101"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(31..2151)
                     /locus_tag="IW671_002304"
     CDS             complement(31..2151)
                     /locus_tag="IW671_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0899275.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2381..3559
                     /locus_tag="IW671_002305"
     CDS             2381..3559
                     /locus_tag="IW671_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0899276.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3575..4333
                     /gene="aroD"
                     /locus_tag="IW671_002306"
     CDS             3575..4333
                     /gene="aroD"
                     /locus_tag="IW671_002306"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0899277.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4356..4841
                     /gene="ybaK"
                     /locus_tag="IW671_002307"
     CDS             4356..4841
                     /gene="ybaK"
                     /locus_tag="IW671_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0899278.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4912..6165)
                     /locus_tag="IW671_002308"
     CDS             complement(4912..6165)
                     /locus_tag="IW671_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0899279.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6282..7631
                     /gene="gdhA"
                     /locus_tag="IW671_002309"
     CDS             6282..7631
                     /gene="gdhA"
                     /locus_tag="IW671_002309"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0899280.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7743..9089
                     /locus_tag="IW671_002310"
     CDS             7743..9089
                     /locus_tag="IW671_002310"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0899281.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9397..9783)
                     /locus_tag="IW671_002311"
     CDS             complement(9397..9783)
                     /locus_tag="IW671_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0899282.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2010 g         2891 t
ORIGIN      
        1 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
       61 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      121 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      181 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      241 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      301 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
      361 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
      421 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
      481 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
      541 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
      601 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
      661 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
      721 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
      781 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
      841 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
      901 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
      961 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1021 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1081 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1141 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1201 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1261 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1321 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     1381 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     1441 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     1501 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     1561 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     1621 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     1681 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     1741 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     1801 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     1861 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     1921 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     1981 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2041 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2101 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2161 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2221 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2281 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     2341 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     2401 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     2461 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     2521 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     2581 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     2641 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     2701 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     2761 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     2821 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     2881 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     2941 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3001 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3061 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3121 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3181 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3241 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3301 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     3361 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     3421 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     3481 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     3541 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     3601 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     3661 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     3721 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     3781 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     3841 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     3901 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     3961 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4021 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4081 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4141 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4201 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4261 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4321 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     4381 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     4441 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     4501 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     4561 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     4621 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     4681 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     4741 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     4801 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     4861 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     4921 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     4981 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5041 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5101 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5161 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5221 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5281 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     5341 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     5401 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     5461 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     5521 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     5581 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     5641 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     5701 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     5761 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     5821 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     5881 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     5941 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6001 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6061 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6121 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6181 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6241 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6301 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     6361 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     6421 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     6481 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     6541 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     6601 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     6661 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     6721 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     6781 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     6841 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     6901 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     6961 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7021 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7081 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7141 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7201 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7261 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7321 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     7381 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     7441 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     7501 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     7561 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     7621 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     7681 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     7741 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     7801 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     7861 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     7921 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     7981 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8041 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8101 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8161 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8221 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8281 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     8341 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     8401 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     8461 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     8521 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     8581 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     8641 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     8701 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     8761 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     8821 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     8881 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     8941 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9001 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9061 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9121 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9181 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9241 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9301 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
     9361 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
     9421 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
     9481 tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga
     9541 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
     9601 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
     9661 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
     9721 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
     9781 catgaaagat ctcctttttt atttttatt
//
LOCUS       DACEKQ010000097         9665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEKQ010000097 DACEKQ010000000
VERSION     DACEKQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.102"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..650)
                     /locus_tag="IW671_002312"
     CDS             complement(<1..650)
                     /locus_tag="IW671_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0899283.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IW671_002313"
     CDS             975..1391
                     /locus_tag="IW671_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0899284.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IW671_002314"
     CDS             1388..1858
                     /locus_tag="IW671_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0899285.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IW671_002315"
     CDS             1872..2645
                     /locus_tag="IW671_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0899286.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IW671_002316"
     CDS             2659..3471
                     /locus_tag="IW671_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0899287.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IW671_002317"
     CDS             3525..4490
                     /locus_tag="IW671_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899288.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IW671_002318"
     CDS             4606..7329
                     /locus_tag="IW671_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0899289.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IW671_002319"
     CDS             7342..7974
                     /locus_tag="IW671_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0899290.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="IW671_002320"
     CDS             8873..>9665
                     /locus_tag="IW671_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0899291.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3261 a         1476 c         1884 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagacca
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaactgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DACEKQ010000098         9560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEKQ010000098 DACEKQ010000000
VERSION     DACEKQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.103"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..349
                     /locus_tag="IW671_002321"
     CDS             <1..349
                     /locus_tag="IW671_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899292.1"
                     /translation="SSNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQ
                     DKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQ
                     KKRDNNDQYLPKK"
     gene            430..1050
                     /locus_tag="IW671_002322"
     CDS             430..1050
                     /locus_tag="IW671_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899293.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1751..2035
                     /locus_tag="IW671_002323"
     CDS             1751..2035
                     /locus_tag="IW671_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0899294.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2038..2955
                     /locus_tag="IW671_002324"
     CDS             2038..2955
                     /locus_tag="IW671_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0899295.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2948..3730
                     /locus_tag="IW671_002325"
     CDS             2948..3730
                     /locus_tag="IW671_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0899296.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3731..4411
                     /locus_tag="IW671_002326"
     CDS             3731..4411
                     /locus_tag="IW671_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899297.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4404..5300
                     /locus_tag="IW671_002327"
     CDS             4404..5300
                     /locus_tag="IW671_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0899298.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5495..7144
                     /locus_tag="IW671_002328"
     CDS             5495..7144
                     /locus_tag="IW671_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0899299.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7738..8673
                     /locus_tag="IW671_002329"
     CDS             7738..8673
                     /locus_tag="IW671_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899300.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8666..9436
                     /locus_tag="IW671_002330"
     CDS             8666..9436
                     /locus_tag="IW671_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899301.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1586 c         2041 g         2877 t
ORIGIN      
        1 ctcgtcaaat ataaaaacta gtacgaaatt aatttgtaaa ttatttaagc ttgataaaat
       61 aagcaatatc gatgatgtaa agtccaagat attaacaaga aaagtaagcg aaccagtaaa
      121 gctaacggca caagataaaa aaaatggaat tgttctttat ggtgatgatg caaagtataa
      181 ctataattgg gatacctcct cagaagctga ggtattaagt attcctacta ccgatacttc
      241 aggggaaaaa gtcaccttta aaataaattt tataacggca atttctaagt tggagtatgg
      301 gaagcaaaaa aagagagata acaatgatca atatctacct aaaaaataac gaattaatga
      361 tgcatatgtt aaagttgttt ttttgaatta aacaactcaa tttattcagt tatatgttca
      421 tcgcagcaag tgaacattaa tcgagttata aaattaatag gagctaaaaa tataaataaa
      481 gttcgatcag aatttatttt tccagaagat ttttttaact ggttttttta tcaattcagt
      541 gaatgcaagc gactcttagg tgagaattat tacttaaaag gaatcggcgg atttatgggg
      601 aatgtggctg atgaacaaaa tattataaaa ggaatatctg aacaaacatc agagcttaca
      661 gtaactaaag catttataag taatggtgaa agtttgaaaa atgtaactgc gcgaattaga
      721 aaagaaaatg aagtagacat tgtttttgga attgaccatg aatgtaattc agtgatatat
      781 gtaacacaat cagcaaattt aattcttttt gagaatggtt gcaaggacga ttttatggtg
      841 gtctatttgt acgatatact aattccaaaa cttcgttcct tatatatgtc ttctgaaagt
      901 atttttgtga gtgaacaaaa gggatctttc tcgaaaaaaa taggtattga agtaattgaa
      961 tcgattatgt gtcataatca tattacgcta gaagaattag aaattttgat agggcaaaaa
     1021 aataataaca cgaaaataca agtttcttag aaatcatttt ttattgagat atctagataa
     1081 atgatttatt cttcaatcaa ccaactcgcc aagctgaggt aatgttttga ggtaatgata
     1141 acatacaact ttttataccc tagtaaaact ctataaacaa agacttatta atttcgcatg
     1201 aaatacattc ctgtaaaatg caataaaata gagttttgtt taaccaggtc ttaagaaagc
     1261 ccgtaaagct tcacagttct caaaacgtta attgcgatta tcgttcgagt tcacgagtta
     1321 cgagaatcgt actgggcgaa gcaatgctga gcgcagttac cttaatccta acttttctgg
     1381 cactttccac aatttggaaa gtgccttttt tgtgttttta gctatgttaa taggtaggat
     1441 tcttggtcta taatatcatt cgatctataa acaaacggga aaaagataaa gtatcatcaa
     1501 acaatattat ttatccgctt ctgaggttgt gacagaagtg gtcagcttca agaaataaga
     1561 aggaatttac aaaaattgct tctcatgttt gataaatatt gtgacttgtg accgaggagt
     1621 tggcaggaga acaccgttta ttcggaagaa gggattgtga aaagtctttt gtcataacct
     1681 cttatttggt agctaattgt aaatcccaaa cggcatgtat tatttttcat aagtccgttt
     1741 ttaaggatta atgttttctt ttgcttttta tatggtctgg cttcagcgag aagttccaac
     1801 gcatatacaa ggacgtctga tgtcaacgat aacaacgatt accatggtgg ccagtccact
     1861 tggattagca ctgtttggtg cgctctttga ttggcagaca actcaaccac gtctgcgaga
     1921 cagtctgatt tttcttgaaa ttcaattgga aaatttacac tttctttata catttctggc
     1981 tgctttcttt atacccgtca actatacttc acttatcaat aagaaagaag gataagtatg
     2041 gattttggaa atgaaacggt cttgaaagca actggtataa ccaaaaaata cggtgctgcc
     2101 aaagcattag ataaagtatc gatcgaaatc aaacgtggaa tgatctatgg tttgattggg
     2161 gagaatggag cgggtaagtc tacctttatg agaactatta tgggtttgat ctcgattgat
     2221 gaaggttcga tcgaattatt tggaacgacg gatttgcagg cagcaagacg acgtatgggt
     2281 caatcgattg aaacccctgc tctttatcca gaattaactg caagagataa cctacgtatc
     2341 caagcagcga acggcggtgt gtcggataga gaaatcgaag acctgctgaa aatgatgcga
     2401 ttggaaaata cagggaaaaa gaaagcaaag aatttctctt taggtatgcg ccagcgttta
     2461 gctattgcaa atgcactgat cacgaatcca gaatttttga ttttggatga accgacgaat
     2521 gggatggatc cagcagggat ggcagaaatg cgagaaatca tccaacgttt ggtaaaagaa
     2581 cggggcatca ccgtcttgct ttctagccac ttgctagatg aattgtcaca gatcgccact
     2641 cactacggta ttttgcatga aggacacttg atcaaagaat tatcgaaaga agaattggca
     2701 caagaatctc gtcaatttat aaaaattgac acttcggcga cggaacaagc agtaacggtt
     2761 ctcgactctc ttggttatag ggattatttt gtccaatcta gccgtgttat ccagcttttt
     2821 gaaggaattg accaagtagc agccatcaat caagcattgg tagaggcaaa agttcctgtg
     2881 gatggtatcc acctagttgg gcaaaaactg gaagattatt tcttacaatt aactggaggc
     2941 aatccacatg tttaatctat taactgctga aaaaataaaa gtatggaaga gccgtaaaat
     3001 gtgggtctca ctaggactga tggtcgtact tccgcttttt tattcttgga acgaatggta
     3061 tatgcataca aaatacggca atgaattaaa ccaagcaacg gatacagtca tcaacggtgc
     3121 aacagggatt ttaatggtgg aaaaaatggc tgcatggatt cttctggctt tcgcagcatt
     3181 tgcgtgcttt tatattgggg aagaatttca aaacggtacg attcgcaata cgttatcttt
     3241 aggacggaat cgaatgactt attatgtgtc gaaacttctc gttacgcttt tgatcacaac
     3301 acttggcgtg gttttgatta cggggctagc catgattgct tacacacttg ctttcggatt
     3361 tggtgaagtt gagggaatca aaaactatgg aaattatgtt ctaaaagtat ttcctgtatt
     3421 gcttctgctg atattagcga cattatctgt tccagtggct ttgacattca tcactagaag
     3481 taccagtgtc agtttgcttc tttcttttct ttatatcatg ggaacggcct tcgttcccgg
     3541 tgttttcgca aaaatcaaag gcttggaatt tctaacggag tggtttacgg aaacgtggct
     3601 catgtatacg gactttgcgc aacaagcaac ttattcgcaa gcaccgaaaa tgattctggt
     3661 cagtctagta acaatcgtgg tatcatcagc tctaggaatg tttattttcc aaaaatctga
     3721 cattaagtag gtgttcctgc tggaaactat tttgattatc gaagacgatg aagctgttca
     3781 ttctttactg gaggaggttc ttgaacagag atacaaactt cttgatgctt attcaggtac
     3841 ggaaggaaaa ttattgttaa gcacatatcc tgtcgatttg atccttctag atctcatgct
     3901 tccagggctt tcaggtgaag cattgttagc agaaatccga cagacatcga atgttccgat
     3961 catcgttctt tcagcaaaaa gcgatcaaag agataaagtt tccttgttgg cagcaggagc
     4021 ggatgactat gtgacgaaac catttgatat cgaagaactg ttgttacgaa tcggcatcca
     4081 acttcgtcat cagacttctg tccaagtaaa agatctgtcg caacgcatct attacaagga
     4141 aatcttactg gacaaagagt cgagggatgt aaatgtgaat ggtgtggctg tccatttgac
     4201 aggaagagaa ttcgatctgc tgaagctttt tttagaacat ccgaaaaaag tattttcgcg
     4261 agcaaatctt tatgaaacag tttggcaaga accatatttt gatagtgaga aaacagtgaa
     4321 tatgcatatt tccaatcttc gagggaaact agcaaaggca catgctgtgt atattcatac
     4381 ggtgtgggga gtggggttta gatttgatta agtggctgtt gcttctcgtt gttatcttta
     4441 tgggaagttt tattttcttc ctgcaaaggg aaatcaagag attaactaag aaaatcgcca
     4501 ctctttctga acgacaagaa tatggtggtc gtctttcagt cgaatttcga gaaaagaatc
     4561 taatggacct agtagatgca ctgaatacat gggtggatga aaatgaacaa aaaggacaag
     4621 catttcaaga aatggaagaa aacatcagac tatcgatcgt cggcctttct catgatctca
     4681 gaactccttt gactgctatt aacggttatg tacaattact ggaacaggta gaggatacgg
     4741 agaaacggga gaagtattta acgatcatca aacaatctgt tgcaagatta ctcagtatga
     4801 cggatcagtt ttatgatctt gctcgaatag agacgaagca aaaagaaatg gatctccata
     4861 cgctttcttt ttctaaagag gtggaagaag tatttttctc tttttatgaa caattttctg
     4921 agaaaaatat cgaggtagcg ttcgctgaac actcgataaa tacaactgta ttagcagatc
     4981 cgctcatgtt gctgcgagtg ctgcaaaata tcatacagaa tttattgcgt tatgcagatg
     5041 gacaagcaaa ggtgacattt tcaaaagagg attcattcat tcgattaaca gtggccaatc
     5101 ctatcaaacc aggagcgcaa acctctgttg aaaaagtctt ccagcgtttt tataccgaaa
     5161 ataccagccg cacaaatacg gaatctagcg gaatcgggct ttacctgtca aaaaaattgg
     5221 tggaaggaat gggaggagag atgaccgcta gattagacgg gggcatcttc tccatctccg
     5281 tcaagttgcg gagggtttaa ttggctctaa tttacctgaa gtgaatgaca aaaaattgta
     5341 tctcgtttta aagacatctt cttttttgga aggtgttttt attttggtgt gattgatacg
     5401 aattaggtaa cgaagacagt tatttttgta cccccttttc aaatagcgca acagtgctat
     5461 actaaaacta acaaatcaag gaacggagag ggatatgggc caaagagaat ttatcgtttt
     5521 aattattatt gcagttgtta ttttattagt tgttttagac attttttcac gtttgaaagt
     5581 aaaggaaaca gttcgtagta attggggaaa aattccttat caaccgagat ttgataaaga
     5641 agaaagtttg aaggatgcgt ggctcacgga gaaaaaattc cgttcatggg atagtgaaat
     5701 cgacgatctt acttggtatg acttggatat gttcgaagta tttgaaggga tcaacagtac
     5761 ctattctagt gtaggttcag aagcgttgta tcagcgttta cgtagctttg attttggaga
     5821 agattatcag ttagaaaaac taattgcttt ttatcaggaa aatcctcaac tgagagaaag
     5881 aatccaatat caattcgctc gtttaggaaa aaaagatcat aattttgcaa aacaatatct
     5941 agcggatgga aaaagtaaaa agattggacg cgtaggttta tttatttttc ttggcttgct
     6001 tccgctggtg ggtgtaggtt tgcttctttt agggcaagta ctaggaatct atctagcaat
     6061 cggtggctta gtattcaata cggtctatta tctagtgaag aaacaaacac tagaaacaga
     6121 gctgaatagc atgagttatt tggtttcaac gatttcagcc gccaatcagt tagcaaaaat
     6181 gacgacacca cttcaaaaag agctgaaaca gcaaactaaa ccattaagag acatccctaa
     6241 attaggtttt tcatttcgaa tgaagagcgg gagtgaagca gaattgattt ttgaatatgt
     6301 caacatcatg ttcatgctgc catttatctc ctatcatttc gttctcacta aattagaaaa
     6361 gcattcaact gaagccatca agctgtggga attgctggga gaattggaag ttgcagcagc
     6421 cgttttgaat tatcgcacct atatgccgat cacttgccga ccagagttta ctgagggcgg
     6481 tgtgacagcg aaagatatct atcatccatt gttaaaagaa gcggtagtta atccagtcaa
     6541 ctggcagaag aatacattag tcacgggttc caatgcatca ggaaaatcta cgtatgtcaa
     6601 aagtatagcc atcagctgta ttctagcaca gacgatccaa acagcgattg cagaacagtt
     6661 taccctgcaa cctggacatg tgttgacttc catggcaatc gaagatgatt tatttgaagg
     6721 cgacagttat tttgtttcag aaatcaaatc gatcaaacgt ttgctggacc aagtggcttc
     6781 caaagaacgc tgctactgct tcgtagatga gattttaaaa ggaacaaata cgatcgaacg
     6841 gattgcttct tccgcttcag tcgttcggtg gcttgcggag tatccatctt tagcatttgt
     6901 cgccacacat gatatcgaat tgacagagat tttaaagaat tattgtgaga atgtccactt
     6961 tgaagaacaa gtaacagaag gaaaaggaat cagttttgat ttcaaattaa aagaaggacc
     7021 ttcaaggacc cgaaatgcga ttgccttgct gaaagttttg aattatccta aatccatagt
     7081 tgaacaggcc caaaaagaaa gtttgttgtt cgatgaacag cgtcagtggt atccgtttga
     7141 ttaactttat caaacgaagc atgatgcgtg ggtccaaaaa gacgattatc aaaagaagta
     7201 cattgatgcc aaaaaagtgg cagaattcaa tgggaacgag tctgctgtcc agatgatctt
     7261 ggaagattta gcaaaagacg atacaaaata aaccacgctg tcttccttct agcagctttt
     7321 atcgaaaaaa cttagcatca gcgacgagac gcacgttcca tggcaaagtt ctccgtaaaa
     7381 caaacagcga aaacagaaat tgaaaataac tatttccatt ttatttattt cggttaaaat
     7441 aaaagaaaaa agctttcgat aaaaggacgg aggaaagctt aacatgaata aaaaagacaa
     7501 gaacctcagt tataaggatc ttctatatgt catcgtagta gttgctgtcg gtacttttat
     7561 gattttaaaa gttccagagt tgtttagata gttattctat cgaagatgat catgaactag
     7621 aaaacacatc tcctctttgt tagcaggtgt gtttttgtgt ggaagccaag ttgttttttc
     7681 atctgctgta ttccatgata caattaagaa tcatgtctat aaggagatag gaaataaatg
     7741 gcaaatgcgc ttgaaataaa taatttgaaa aagatctatg gaagtggggt cgaagcattg
     7801 cggggaatcg atctttcggt gaaagaagga gatttctatg cgctattagg gccaaatgga
     7861 gctgggaagt ctacaacgat cgggattatt acttctttag tcaataaaac atctggaaaa
     7921 gtgaatgtct tcggttatga tctggacaag gatctagttc gagcaaagca acagattgga
     7981 ttggttccac aagagttcaa ttttaaccct tttgaaacgg tccagcaaat cgtcgtgaat
     8041 caagcaggct actacggtgt ctctcgcaaa gaagcgatga aacgcagcga aaaatatttg
     8101 aagcagtcca atctttggga aaagagaaat gtccgtgcac gtatgctttc cgggggaatg
     8161 aagcgaagac tgatgattgc tagggcattg atgcatgaac cacgtttgct gatcttggat
     8221 gaaccaacag caggagttga catcgagttg agacgcgaga tgtgggagtt tctacgagaa
     8281 ttgaacgaaa atggtacaac gatcattttg acgacgcatt atctagaaga agctgaaatg
     8341 ctttgtcgca acatcggaat catccaatcg ggtgaactga tcgagaatac aagtatgaaa
     8401 gagcttctgt caaaactgca atttgaaaca tttatctttg atttagcgcc atatgatacc
     8461 aaacctgtga ttgaaggata tgcttgtaca tttgaagatg agcagacaat cgctgtcgaa
     8521 gttgaacgaa atcaaggggt gaacggaatc ttcgatcagc ttacaagaca aggaatcaaa
     8581 gttctttcta tgaggaataa gtcgaatcgt ttagaagaac tgttcttgaa aatcacagaa
     8641 gaaaataatc atacggagga gaaaaatgtt taatctttat tttactgcat taaaaagttt
     8701 agctatcaag gaaacgaacc gctatttgcg tatatgggtc caaacactgg ttccgccagt
     8761 gatcacgaca tccttgtatt ttgttatttt cggaaaaatg atcggtgggc ggatcggtga
     8821 catgggtggt ttttcatata tggaattcat cgttcctggt ttgattatga tgtctgctat
     8881 cactagttcg tatgccaatg tttcttcgtc cttcttctct caaaaatttc agaaaaatat
     8941 cgaagaaatc ttggtagcac ctgttcctac gcatgtgatt atttggggct ttgtgattgg
     9001 tggtgtgggt cgcagtattc tcgtcggctc tctcgtaacg atcatttcct tgttctttgt
     9061 accacttcat gtgtattcat ggttcatggt catcattaca ttattgatga cggcaatctt
     9121 gttttcatta gcaggactgc tcaacgggat ttttgctcag tcttatgatg atgtatcgat
     9181 cgtgcctaca tttgtcttgc agccgttaac ttatctaggt ggcgttttct atgccatctc
     9241 tatgttaccg cccttttggc aagctgtttc aaaagtcaat ccgatcgttt atatgatctc
     9301 cggtttccgt tacggattcc ttggaacgat cgatgtgcca gtagcaacat cgatgatcgt
     9361 acttgtgtta ttcatcgttg ttttgtacgc cgttacttgg tatttgatca ataaaggaag
     9421 aggattgaga agttagaaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
     9481 acaaacggga aaatagttcc tcatattttg ttgttttttg agcgaatacc gagggtcagc
     9541 tgattgaaca ccgtttagtg
//
LOCUS       DACEKQ010000099         9315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEKQ010000099 DACEKQ010000000
VERSION     DACEKQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.104"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            268..888
                     /locus_tag="IW671_002331"
     CDS             268..888
                     /locus_tag="IW671_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899302.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1581..1961
                     /locus_tag="IW671_002332"
     CDS             1581..1961
                     /locus_tag="IW671_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0899303.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1943..2857
                     /locus_tag="IW671_002333"
     CDS             1943..2857
                     /locus_tag="IW671_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0899304.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3097..3786
                     /locus_tag="IW671_002334"
     CDS             3097..3786
                     /locus_tag="IW671_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899305.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3906..3992)
                     /locus_tag="IW671_002335"
     CDS             complement(3906..3992)
                     /locus_tag="IW671_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0899306.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            4637..5374
                     /locus_tag="IW671_002336"
     CDS             4637..5374
                     /locus_tag="IW671_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0899307.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            5402..5941
                     /locus_tag="IW671_002337"
     CDS             5402..5941
                     /locus_tag="IW671_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0899308.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(6439..6900)
                     /locus_tag="IW671_002338"
     CDS             complement(6439..6900)
                     /locus_tag="IW671_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899309.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            7033..7407
                     /locus_tag="IW671_002339"
     CDS             7033..7407
                     /locus_tag="IW671_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ0899310.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            7461..>9315
                     /locus_tag="IW671_002340"
     CDS             7461..>9315
                     /locus_tag="IW671_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899311.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIII"
BASE COUNT         3595 a         1414 c         1455 g         2851 t
ORIGIN      
        1 ttttcaaatg atttttgtta gttttactat aaattcaaaa aaaatagctt taaaaaaaat
       61 atttggatat tcaccaataa gtcaagtttt agatttatgt ttactccctt ttacaataga
      121 gttatttgct actatgtgtt ttcaattgat attttcaaaa aacacttact tgtattttat
      181 aatttttatg atctttttaa taaatctaat tacttctatt attatttata aaaaaaatag
      241 ttttaaatat tttaaggaga atattttatg atattatcag ttgagaattt caccctgtca
      301 attaataaaa aaaagatcaa acaaaattgg aacttttcaa tatcagaaaa agattttatt
      361 atagtaacag gaaaaagtgg tagtggaaaa actactctgt taaataattt aagcctaata
      421 gataaacaat ataccggtaa cttattctat tttgaaaaga aagtttctaa acatgatata
      481 caacgtctac gaagaaatca tattagctat ttatttcaaa actatgctct tcttgaaaat
      541 caaacagtac aatacaattt cgaattagca aaaaagttta acagtaatat ttccaatgat
      601 catatttata atttattaac agatttttct ttgccaaaag atatcttaaa agaaaaaatc
      661 tttctcttaa gcggtggaga acaacaaaga atagcattaa ttagaaatat gttaaaacca
      721 tttgatatat tatttgcaga tgaacctaca ggaaatttag ataaagaaaa ctcaactttt
      781 ataatgaact ttctgaaaaa tttaacagaa aaagaaaaaa aagccgttgt actagtaaca
      841 catgacttaa atgttctaaa ctatggaact aatattatag aattataatt tttacccgtg
      901 atgctagctg atttaaaact agcaccacgg gtatttattt tatattccat ctctcctatc
      961 ccaaaattta aataatcact taataaaaag gaggcaccca agtgatttta ttgaaaaaag
     1021 agtatgaaaa attttatgat agacttattt attctttgca attgccaaaa aaattaaacc
     1081 gtcaattaca aaagacttat gaaactaaat caactgaaga atgggagagt tattgctaga
     1141 tttttggtag aagtaacaaa aagtggacat attatcataa tgactcgccg aaagtctgat
     1201 aaaaaattgg ctaaaaatat tgctgaatgt ttaataaaac tatcagtttt agctaaatag
     1261 ttagtagttt acacaaaaaa gcatttatgt tttttgttgc aaagcaacaa tttcatcttc
     1321 ggtgaaataa gcgaaacttt acaaaaagtg gctagcattt gctagctttt tttgtattgt
     1381 ggagcgtggc ttttgagggg gattcaaggg ggagcttgcg cccctttgac aagcggttat
     1441 ttgcactaaa tacgtaggta tttggctagc aaatatagag cttgccaaac ccttttagtg
     1501 agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg tccgtaggac
     1561 gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc aattcttcta
     1621 aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc gaatccgaat
     1681 ttgcaaagct gaagcaatcg gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag
     1741 ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt gcccaggcaa
     1801 tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgctaag tggtgcaata
     1861 cacaccaaca tgacgttgct tctggtgacg ctcaacgttt atccgaaaac ctaaaaatca
     1921 tgcaaaagga gctacaaaag atatggcaac aattaaggta agccgaacca cttcttgtag
     1981 tcgtgcaatt aattacgcag aaccacgagc aaccattaaa acaggaataa actgtgacgt
     2041 cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag acgaccgtgt
     2101 acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg aaaaagctaa
     2161 tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaatag ctatttacac
     2221 acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta atctggagac
     2281 tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg acctaaatga
     2341 cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaaac cagaaaaacg
     2401 aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag acgaaattcg
     2461 gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc aattttgtaa
     2521 ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca cttacgaatt
     2581 gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg aaaaggagac
     2641 gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa ccaacgagct
     2701 acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag aacaacgaac
     2761 tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac aacacgttga
     2821 aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac atagaaaacg
     2881 aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata caaactacga
     2941 ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat aaggaacaga
     3001 aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc ttagtcctga
     3061 cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt accctatcta
     3121 ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt tcaaacaaga
     3181 tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg ctgtatattt
     3241 tccttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa gcgcgtttct
     3301 agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga taaaaagaaa
     3361 aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttatga ggaaatgaac
     3421 gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa actcactcgt
     3481 ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta tgagcgaacc
     3541 aaaaaagacg tgcaatatgt aaaagataca aagatagaag aagcctgtag tgatctattt
     3601 ggagacacaa acccttggtt tgccagtgaa gaagaaaaaa aagaaaaaca acaatctgtc
     3661 acatatgaac gctatgaaat cttacttcaa caagcaacta acatgcaaaa tccgacaaaa
     3721 aaagaaaccg ccttaaacta tctaaaaagt gtcaaggaat ggttagatca gcaacaacaa
     3781 aattgaattt gatttaaaat catgttaaac tgaatgtgca aatgatcaaa cattagatac
     3841 acgaaagccc cagagaggtc ggattctctg gggctttttt caggttgatg tcgcctttat
     3901 tgattttact tcttgtttcg gttattcaac caataactga ataaagagac acaaactcct
     3961 acaagaaggg ggcaaatgaa cagttcaaac attagataca cctccttcca gtcgcaagac
     4021 tgtcggatag gcgacaagtt attatatctt aaaaaattaa atttgtgtgg tgtttttaag
     4081 tatcacttgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg cttctcgact
     4141 agattaaggg aaattaatca agaattgtct tagaacggct tagacaagct ttcagagtct
     4201 cacacctcct ctttgaacaa cggttttagc aaaggagccg gaggcgactg cgcagtataa
     4261 tatgagagca tagcgatcac acctttttag cgaccgtagg gagcaagttt tccagtttag
     4321 aaaaattcgc gaaccttttt ggaggaaaaa gtaggagttt tttaggcggg aaattcaatg
     4381 tgctcttaag atatactgtt agatatactg ttagatatac tgttaataat aactgttagg
     4441 gattagcaac cttatgggag agtaagtatt gacataggta gtgggggata aattgtcaca
     4501 ctatggggga taaattgtca cactatgggg gataaattgt cacactatgg gggataaatt
     4561 gtcacactat gggggataaa ttgtgtttta catcctctta tgtctatgat aacataattt
     4621 attgaggtga tttttattga gtaacgatat agttcgatat aataatggat taaattctgt
     4681 ccctttaaga aaatttaccc ctatagatat ggatttattt tggggaatat gttctaaaat
     4741 gaaaagaaaa ggtacaaaag aagaaatttt tacatttaaa caaataaaag aattaattga
     4801 ttacaatata aatgcacgtg gcgaccgttt tgctaatgat ttaataaaaa tgtcaaaaaa
     4861 aatcattact ttgtctttta tttatgaaga cgaaaaaaca tatgaacaat taaatctgtt
     4921 tcaaaaattt aaaatcgata aagtgaatga aacattatta ataaaggttt ctgaagaatt
     4981 tgaatatata cttaattcaa tcggaactaa ttttactcgt ttcgaattag aaaatatgac
     5041 aaacttaaag agtagctacg ttaaagagtt atatcgccaa cttatggctc ataaagacag
     5101 aagatctaaa aaaggtcact ggtttgtaaa aatagaagat tttagagctc tattagcaat
     5161 accagaaagt tatcgcatga gagatattga taaacaaatt tttgcccaag cgaaaaaaga
     5221 atttttaaac gttaattcaa ctcaccctgc tatttttaaa acttttgaaa tcaaaaaaat
     5281 taaagcctac aaaggaaata agataggatc tctgcaaatt tattttgaag aaaatatacc
     5341 ttcaattacc ttagataatt ggttagagaa ataataccta acaatttagg aggcaattat
     5401 catgcaaccc aaagaaacct ttacaataaa ggaattatct gaattatttg aaataagtag
     5461 gcaagcaatc tctaaacatg ttgataaatt agacccgtcc ttattagcta aaaatgagcg
     5521 tggttataag gttgtattac gttctggagt attgcaactt gctagcaact taggtaacca
     5581 aaggttacta tataggttgc atgagtatga acaagaaaac gataaaaacg tttatatatc
     5641 aagcaaaaac gtgattgtaa acgagttgct agataagttg cgtgaagaaa atgaattttt
     5701 acgagaacaa ctttccctca aagagcaaaa taattctaat atgcaaaaat tactcgacca
     5761 gcaacaacag ctcaccctac aggcaaatca acagatcgaa aaactgcaag aacagttaca
     5821 attagcttat acggaagaaa ctccagaaga tgaatcaact actattcttt ctgaagaaac
     5881 agaatcgata gaaaaacaca caaaaaagaa gaagcaaaaa tggtggcaac tttggaaata
     5941 actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt tactaggaat
     6001 tctaggcttc agtccgatgt tattgtcagt aatcaataaa gggaataaac atggtgaatt
     6061 gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa aagagacgga
     6121 taaataataa aaaagactgc tcttccgagc agccagttaa aaaataatga gtgtttattc
     6181 ttgaaaaagc acgcgcttat gaagcgctac caaaaattta ccatatttta caaaaaataa
     6241 caagtcttaa aaaaatgaca gataaaaatg cacaaagaat tagatataga taaccgcgaa
     6301 aaatcaagct ttagaaagtt gcttttagct agaccaatta aaaatcacaa agtgagcgtc
     6361 tctatccatt ggtagagggg ctacttggca tagtgtacat tatgtcaact atgcaattaa
     6421 ataactaact acttcatatt agcttaataa aataatcaaa ataactaggg ggaaaaacat
     6481 tgtataaaaa aatttttcat tttttaaata agaatagtaa atataaaaat aatttaagat
     6541 atctattact attaatccag atagagcaaa ccaagtaaat attccatgtg taaagctaaa
     6601 aaaattcatt tgaatcaatt caaataaacc taatatagta acaaatatat taaaaataat
     6661 agctgtcaat accagtaaat tatttttttt attttttgaa ttatcttcat ttaaaataga
     6721 atttatgtta gtactataaa attcacaaat tttttttagt gtctctatat ctggtgttgt
     6781 tttgttattt tcccactttg aaatagtttg acgtgtaacg ttaaattttc ttgccacata
     6841 ttcttgagta tacccttttt tcactcttaa ttctcttagc ttagatccta aatcctccat
     6901 tttttctcct atgtaaacga ttatttacat aaaattttat cataaaaata ctaacgcgcc
     6961 aaaaaagttc tttattttat aaaacacact aaaataaaat aattctagta aaaaagatag
     7021 gagaaataag ttatgaaaaa atttttatgt ctttctgctt taatgggtgt cttattaact
     7081 agtggaggaa tagctagtgc cacagaagct ttaaatctag atgtacccga agaccatacc
     7141 gccatttacg gaggaggaat ggaagctaca gaagaaggat tttcttctag taaattgaga
     7201 tatgctgctg gcggcgggga tttcaactgt ggggttaatg gatttaaagt ttatgcaaat
     7261 tactaccatg ctagagctaa acatagtgca acagctaaaa atggacgagg cggtcaagta
     7321 cgatcagttc agaaagctgg agtaagagct tatgcaacat gcaatgccac acttactgga
     7381 aatactggtt ggtggaatgt ttattaaaaa agagagttta aactctcttt tttatttaca
     7441 tttgaaagga aattcaaaat atgttaaaaa aaataaacgc tgttttatca ctttttgtct
     7501 gcattacaat actatttttt tctactaatg ttataaatgg tataaagact tctgaagcgc
     7561 tatataaaaa taccgctgaa attcatatgc aatataaaga tgatatggac tttaacagtt
     7621 atataaacgc tttgtatgaa atatctacac aagaaaatat taatatttct caatatagtt
     7681 ttactgctgt taaccaacta actattcttg ctacaaaccc taacgctaac aaacactgga
     7741 aattttctaa aaaaaatata ttagcaagaa acgattctaa agtccattat actaataaca
     7801 aaaattctaa gtaccaatta aaacttccaa ataatttttt aaatataaat atttatcctt
     7861 tttctcatgt ggaaaatatt ggattaagtg aaatattata tgtacaagga aactcaaaca
     7921 aattaattcc tgtactgaaa aaatatggaa cacctactat ttccaatatc aatcaatcag
     7981 atacttttca aattaatact aatattgttc ttgttatctg ctatcttagt atatttattg
     8041 taattactac aattatattt gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt
     8101 taggctataa ctcttttgat actataaaag attctattaa agagttaaac ctcttatttt
     8161 gttctttgat tataactttc attttatcta tttttttgag catatattac agtaacgaaa
     8221 attttttcct atcatttctt ctatgtagta tttttcattt tataattagt tttttattta
     8281 tcatagttac tctaagtatc tatcatttat ctagtataaa aaattatcta aaaaattcac
     8341 gaccacttaa gttaaatctt tgtatattaa atatctgtct attcttaagc atgatccttc
     8401 tgttaatagc ttctacaaaa gtaataaata cccataatga agccgaaaat aattctctta
     8461 aatattggga aagaactact aatttataca aaacaaatat tactaatcaa ttaaatagaa
     8521 acaacaccgt agaagaaaat aactacttga aaaaagctag taaatttgtt tataaaatac
     8581 agaagaatta taaaactttt attatcgcgc catacaatta cgcaactatc caagagaata
     8641 ataaagaatt ttttattgga caaaaagttt atccaaattt tgaagactat gttagtcaac
     8701 ctgctggtgc aggaatatgt gttgatttaa actatctaaa atattacaat cctatttctt
     8761 ttgaagaatc tactgactta aatttaataa actctgatcc actaactact tacattctag
     8821 tacctaaaaa atataaagaa tatgcaaaaa tgattgaaaa aaattattta gaagacatac
     8881 agtttagact tgaggactct cctcctcaag ctccttataa aaatcctaat ttgaaaaatt
     8941 taaaaataaa actaatattt gttaataata accaaaagta ttttagtttt aataccttat
     9001 atggaaaggc aaaagataac tatacaataa ctgatcccat agttagagtt attaatccag
     9061 aaataacaga atcattattt tggggaaata tattgactag tgatggtgga ttattttttg
     9121 atcagaaaca tacttcaaac caaaactttt ttaatcaaat taatccgtat ataaaagaat
     9181 ttaacctaga aaatatcata aatttttctt attctgtttt tcaagaaact ggtgaattat
     9241 tatctcaaaa aaaagtaaat aaattaaatg catatctaca atatctttta atattaggat
     9301 tatttataat aatta
//
LOCUS       DACEKQ010000100         9267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEKQ010000100 DACEKQ010000000
VERSION     DACEKQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.105"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            472..2109
                     /locus_tag="IW671_002341"
     CDS             472..2109
                     /locus_tag="IW671_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0899312.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW671_002342"
     CDS             complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW671_002342"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0899313.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3185..3820)
                     /locus_tag="IW671_002343"
     CDS             complement(3185..3820)
                     /locus_tag="IW671_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0899314.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW671_002344"
     CDS             complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW671_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0899315.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5406..6402
                     /locus_tag="IW671_002345"
                     /pseudo
     CDS             5406..6402
                     /locus_tag="IW671_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6418..8436
                     /locus_tag="IW671_002346"
     CDS             6418..8436
                     /locus_tag="IW671_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0899316.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8496..9212)
                     /locus_tag="IW671_002347"
     CDS             complement(8496..9212)
                     /locus_tag="IW671_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0899317.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT         3001 a         1716 c         1452 g         3098 t
ORIGIN      
        1 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
       61 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
      121 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
      181 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
      241 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
      301 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
      361 agtatactag aattgtcttt ttttaataag ttttgcgtta aatttttcat agcactagta
      421 tgatatacta gaaaagaatc aatttttatt acttttaaat aggagaaaaa aatgaaaaaa
      481 aacaaaaacc aaataataga tatcattttt atgttatttc taagttttgc ttacgtcatc
      541 cctctattca tgtctatggg aatctatcat agtgtcaatc aggatactta ctttcattta
      601 tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa taacttcgat
      661 catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc agctttttta
      721 ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt ttttatcact
      781 tttttaacaa tggtcgtttc ttatttttct atgaaacaaa taaaaaacaa tcgttatatt
      841 tcgctgttat tttcgattat ttatacattt tccgcctatc gtgccattga catattccat
      901 cgtgcttcat tgggagaagc tgtcgcttta acatttttgc ctttgatact aatggggtgt
      961 tacgaaattt atattagaga ttaccaaaaa tggtactggt tgtctattgg catgacgcta
     1021 gtagtgtata ctcatttact ttctgtcgct atggtttctg tatttattgg aggaacgttg
     1081 tttctttctt tttatttttg ggaccaaaaa atcgctagac tcttaagtct tttaaaagct
     1141 accgtcctca cctttttttt aagtgcggga ttcttaattc cttttattca acaatctaga
     1201 gcacaagaat tgaaagtccc actgggaaaa gaattaagtg gtatggctcc tagtgatatg
     1261 ctgacacaca ttttaaataa taactataac aactatacta ttggattatt cttatttctc
     1321 ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt atttattttc
     1381 tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca actttttaac
     1441 catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc ttctttattt
     1501 atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa taattcatgg
     1561 aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt aagcaattct
     1621 atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt tgctatagct
     1681 gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc tgatatgatt
     1741 aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca ttttatgagt
     1801 aacaaactta caattgaatt agataattcg aataataaaa atactgtctt gacgacacct
     1861 atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca aacaaaatta
     1921 tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt cgttattact
     1981 tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac tttgatttta
     2041 tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga agtacaacat
     2101 tctcactaga tttttattta agaaagactt tttagctgta cacataaaca ggctaaaaag
     2161 tcttttttta tatttcttta atttgcttca tacattattt aacatcgata tttatactgt
     2221 gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt catccaaaaa
     2281 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
     2341 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
     2401 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
     2461 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
     2521 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
     2581 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
     2641 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
     2701 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
     2761 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
     2821 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
     2881 ctgatgcaat actttttcta tttttccttg aatggtttta tcgtcatctc caagtctttg
     2941 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
     3001 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
     3061 atagctcctt tcttctctaa aacagaagct atacaagaaa actgtggaaa tatatatttc
     3121 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
     3181 agttttactg tttgagatgc aagatactca accaggcaat gggcacataa ttaaagaaaa
     3241 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
     3301 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
     3361 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
     3421 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
     3481 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
     3541 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtactt acaaatgcag
     3601 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
     3661 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
     3721 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
     3781 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
     3841 tacctatgct tttaattgtt attataaacg actttatctt gatttaacag aaacctctat
     3901 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
     3961 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
     4021 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
     4081 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga cgatttgcat
     4141 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
     4201 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
     4261 caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
     4321 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
     4381 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
     4441 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
     4501 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
     4561 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag cgattccagt
     4621 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
     4681 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcgattctga
     4741 acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa ggtaattcgc
     4801 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
     4861 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
     4921 aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta aatataagat
     4981 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
     5041 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa
     5101 tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga ctaaaatacc
     5161 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
     5221 tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta taattttcta
     5281 aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc tgtctacacg
     5341 ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc aggaaacgtt
     5401 ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg
     5461 agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta cagcgtatat
     5521 tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc tacatctata
     5581 catttttaca aacttccgtt gttattggtg caaatttgat cccactttac atgggttacc
     5641 aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt gcctttatat
     5701 tcctaatagc cacactagtt tgcaatatct tttttttacg tacaagctgg cagcttgaat
     5761 attcgtgatt attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt
     5821 tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg
     5881 aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact
     5941 ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat
     6001 ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag
     6061 cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa
     6121 atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct
     6181 ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa
     6241 ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata
     6301 tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac
     6361 tcagggaagg catggctgat aaatatcggg cagtttttta tttgctattt attagcagtg
     6421 tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc
     6481 gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat
     6541 tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc
     6601 gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta
     6661 aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt
     6721 ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc
     6781 attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc
     6841 tttccttcag atttaaaaat gctgaccgga ttgtcagaac tattatcgat ggttagccca
     6901 gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta
     6961 caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt
     7021 atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct
     7081 tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc
     7141 gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa
     7201 gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag
     7261 gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat
     7321 ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct
     7381 attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt
     7441 tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac
     7501 ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca
     7561 aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat
     7621 cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg
     7681 gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca
     7741 tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa cgtcacaatt catccaatta
     7801 tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct
     7861 gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt
     7921 cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg
     7981 attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa
     8041 aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt
     8101 gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct
     8161 cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat
     8221 catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa
     8281 caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa
     8341 aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac
     8401 agtgcaacat gggcgacaca aaaaataaaa gactaacaaa taaactgagc aaggggttgt
     8461 gataaaagac atgtcacaac cccttgttta tctcattaat ttgcttgtcc ataaacgatt
     8521 tgattaaagc catttttcaa ttcattattc gctgtctcgt tgaagtaaag ataattatcg
     8581 ggattcccct gattttgttg tgtcaccaat acttttggct gattttgctg aaacccgaat
     8641 agatagacgt gctttttcag atatggttct gtctctgcat cggaatagac ggctgtgatt
     8701 tgatagtctg cttgacctgt cccactttct gaccattctg cagaaacagg cactccacca
     8761 atagccatct tccactcccc gttcaaaata gcttgaggga ctttaagacc atataagtcc
     8821 acactcattt gattcgtata acttttgtat tgttgtccca ttgtttggcc ccatttggtc
     8881 ataaatgcct ctaattgtgc ggatttgtct gcattccaca gtgtttgttc aacttgttcg
     8941 ctgctttctg aagaggaaga tgaagcctct acagaagaac ttgaagtaga tgtttgagtt
     9001 gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct
     9061 gcagttgact tgctctctgt gctagttgtt gtagtagttg caactgttgc tttcgctgtt
     9121 tcttttgttg attctttttt cgattcattc ccgttggtac aaccagctaa caaaaaagct
     9181 agaccaattg ttactaaaat cctttttttc aattcgccgt ttgtataatt aagcgagaca
     9241 aataaaaaag ccatttatgt tacactc
//
LOCUS       DACEKQ010000101         9248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEKQ010000101 DACEKQ010000000
VERSION     DACEKQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.106"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            317..1102
                     /locus_tag="IW671_002348"
     CDS             317..1102
                     /locus_tag="IW671_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0899318.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="IW671_002349"
     CDS             1115..1966
                     /locus_tag="IW671_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0899319.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="IW671_002350"
     CDS             complement(2090..2542)
                     /locus_tag="IW671_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899320.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="IW671_002351"
     CDS             complement(3007..3375)
                     /locus_tag="IW671_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0899321.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="IW671_002352"
     CDS             3525..4007
                     /locus_tag="IW671_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0899322.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="IW671_002353"
     CDS             4044..5054
                     /locus_tag="IW671_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0899323.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5169..5882)
                     /locus_tag="IW671_002354"
     CDS             complement(5169..5882)
                     /locus_tag="IW671_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899324.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6072..6662)
                     /locus_tag="IW671_002355"
     CDS             complement(6072..6662)
                     /locus_tag="IW671_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0899325.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7185..7856
                     /locus_tag="IW671_002356"
     CDS             7185..7856
                     /locus_tag="IW671_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0899326.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            8498..>9248
                     /locus_tag="IW671_002357"
     CDS             8498..>9248
                     /locus_tag="IW671_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAQ0899327.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYK"
BASE COUNT         3186 a         1429 c         1750 g         2883 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttgccag cttctttttg tatgatctat
     5161 tagaaatttt aattatccat aactatcaca ttgatcagct gtttcacatc atttggatta
     5221 cctgtaggaa tagctactac agatgcatta tcaataattt cttgagttgt ttctctaatg
     5281 aacgctggat aagatgtttg atctgcaggc atttgccaat gtgtaggaac tggataacta
     5341 gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca
     5401 gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt
     5461 gttcctgcag gataatgaat aacatgtagt tcgaaatcac tcgatgtatc tctaaaagta
     5521 ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct
     5581 aacggatcta ttgactgaga agactgtgag gaagttgtgt ctattgttga agaagaaata
     5641 tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt
     5701 gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa
     5761 ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta
     5821 ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc taactttttc
     5881 atataaaata cctcattatt tttttaatac cagtatagcg taatcctgaa tttttgaatc
     5941 cgtaaaacct tgtctatgaa tataaataga gtatatcttc tttgataaat gtcagaaaaa
     6001 gtgtgacaaa taaccatcag taaaattact tgtcatacta ttgagaagga gtatttaatt
     6061 gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt
     6121 ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga
     6181 atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc
     6241 acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga
     6301 aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca
     6361 actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga
     6421 tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact
     6481 ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg
     6541 acctctgatt ggatagaagc agttttgttt tgccatccct ctttttggtt tagtgtgcta
     6601 gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc
     6661 attttcaagc tctcctttac tatctatgac aaggacaaca gttttaacat acagacattc
     6721 attaaatttg gatagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag
     6781 acagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc
     6841 agcattataa cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta
     6901 caatcttaaa agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc
     6961 gggcactcag acatgaaaac aaccctagaa atatataact agtccaatga aaaaacagcg
     7021 aatgccgtag aaaacaagga ttctgcgtac attcatatct cattttgcga gcatatctgc
     7081 tattactgtg actaaatgtt aatatggtta aaatgaccgt tatatttttt atataaatat
     7141 cgtataatta gtatgaatat agaagaagga aagaaggtgc aatcttggaa cctaaagaca
     7201 agtatttaga ttacgctagg gtcaataaag ataaatttat tgaaacaatc atcaagggaa
     7261 aaatccctga tgatgaaaaa gatgcggtat tcatggctgg aagcccagga gcaggtaaaa
     7321 cagaggttgc actaggttta gctgaaaatt atgacaacca tgttgtgatt gatgcggact
     7381 attttagagc acaatttcca gaatacaatg gaaagaactc aagtgttttt cagaaagctt
     7441 cttcttggtt agttgaacaa tcattaaaat atgtactgga acatggctat tctttcatac
     7501 tagaggatac ttttgctatc cttagtgcag agaaaaatat tattcgagcc cttaaaaata
     7561 actttcgcgt tacgattttc tatgtttacc aagatcccaa agttgcctgg gatttcactc
     7621 gaaaacgtga actagctgaa ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt
     7681 ttaagtccag agagaatatt gaaaaagtca aaaatagaca tcctgaagta gtccttcaca
     7741 taatgattaa ggattatcaa aacgacattt ctgaagtgca ttatgcagca gataatattc
     7801 aattagtact acccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     7861 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     7921 aaacgcctca gcgaaagaat ttaaaacagt aatgaagtac gtagcagatg aagcaaatcg
     7981 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     8041 ttgagttttt tgttttaatg aattaagact attaaataga taattattgt aacttaaaaa
     8101 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     8161 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     8221 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     8281 tgtttttaca taagaagact tggggtattt tttagtttga ttagctctga tatacctata
     8341 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatgtt
     8401 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     8461 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     8521 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctgatt taattagaaa
     8581 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     8641 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     8701 ttcagagaag tttaaatgtt tcaaacaggg tggaatgaga agatctcatc aaactaatac
     8761 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     8821 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     8881 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     8941 accaaaagaa cacacgtatc tgggagaagt atatttagca ggacaaatat atactgttaa
     9001 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     9061 tcaagagcaa ttgattgaag ataaagatat atataaccag gaagaaaatc aaactagaca
     9121 tattcgtaat ttatctgatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
     9181 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
     9241 atataaac
//
LOCUS       DACEKQ010000102         9221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEKQ010000102 DACEKQ010000000
VERSION     DACEKQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.107"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..1179)
                     /locus_tag="IW671_002358"
     CDS             complement(94..1179)
                     /locus_tag="IW671_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0899328.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1499..1906)
                     /locus_tag="IW671_002359"
     CDS             complement(1499..1906)
                     /locus_tag="IW671_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899329.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1925..2743)
                     /locus_tag="IW671_002360"
     CDS             complement(1925..2743)
                     /locus_tag="IW671_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0899330.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(2969..3262)
                     /locus_tag="IW671_002361"
     CDS             complement(2969..3262)
                     /locus_tag="IW671_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0899331.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(3398..3577)
                     /locus_tag="IW671_002362"
     CDS             complement(3398..3577)
                     /locus_tag="IW671_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899332.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(3574..3924)
                     /locus_tag="IW671_002363"
     CDS             complement(3574..3924)
                     /locus_tag="IW671_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899333.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3917..5242)
                     /locus_tag="IW671_002364"
     CDS             complement(3917..5242)
                     /locus_tag="IW671_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0899334.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(5351..5554)
                     /locus_tag="IW671_002365"
     CDS             complement(5351..5554)
                     /locus_tag="IW671_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0899335.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            complement(5714..6289)
                     /locus_tag="IW671_002366"
     CDS             complement(5714..6289)
                     /locus_tag="IW671_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899336.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            complement(6305..6910)
                     /locus_tag="IW671_002367"
     CDS             complement(6305..6910)
                     /locus_tag="IW671_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAQ0899337.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(7516..7695)
                     /locus_tag="IW671_002368"
     CDS             complement(7516..7695)
                     /locus_tag="IW671_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899338.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFNIPALKK"
     gene            complement(8076..8783)
                     /locus_tag="IW671_002369"
     CDS             complement(8076..8783)
                     /locus_tag="IW671_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899339.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
     gene            complement(8776..9213)
                     /locus_tag="IW671_002370"
     CDS             complement(8776..9213)
                     /locus_tag="IW671_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899340.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
BASE COUNT         2634 a         1719 c         1365 g         3503 t
ORIGIN      
        1 aactatttat ttgtaacatt tgtaataaaa ttaaaattat aaaaaagttc taatttaaaa
       61 ttgatcattt ttatagtgat cgctttttat caactactct agccaatttt ccgtactaac
      121 tgttggtaat tcgttaaatg tttctctttg ttcgtttgca agctttgccg caatagcttt
      181 ttcaataaac cagtttttta catacacaaa aatcaaattc tgcaaattat ttactttctc
      241 agtcttttgt ttttgataag ctttcagcaa cgtcatatat aatcctttat ccgcatcaat
      301 tccgtaagat tcaagttctt catacacaac taccgtcttc tcaagttcct cggctttatg
      361 ttttgcgttg tgtattgtgg tgacggtttg ttgggcttct tcgtaagtgt tagaaaatgt
      421 ttttatcaag cttaatacct tttctggtac aaatgtgttt atgctgtctt ctttcatgat
      481 ttcaacgaag ttatctagca aaacttgatt ttgtaattgg tctgtttcag tgtcttttaa
      541 gtgtctttta gtgtctaaag atttattaat ataataatct tgtgcgattt ccgcatttcc
      601 agtgttatca agggtttcag ctgttttttc tcgacgtgcg atttccgcat ttccagtatt
      661 atcaagggtt ttagcagttt cctctcgacg tgcgatttcc gcattccgag aagaggttga
      721 tttatcaata gtttggctta aatcgtcatc gaaagaattc tgaataaaaa gatcatcggc
      781 acttatttca ggttgaagta aatagtattt atttttttca ttttttttcg tttttgggtt
      841 aaattctcct tttacgactg aaatcaaatt atatgtttct aactcgttta aaatggtttt
      901 tactttgcct tggtagcaat ttaatttgtc cattaggtct gcttgtgtaa attccaaata
      961 taatagacca tcatcgtcga tccatttttc atgtaaggct ctacgaaatt catttttaaa
     1021 aagcatatag gctatttttg agctgtcttt cattttagag tatttagaat tctgaattaa
     1081 aaaactagga aatttataat atttttcaat ataaacattc gttgctttat atccccaatt
     1141 tttaataatc attttttgag attgaatttc tgtagacatt aaatttctcc ttcttcatag
     1201 aaacttgtat atattcacga ttttcaacaa gtgaactata ccatgtatta agggtgaata
     1261 aaacactttc agtatttaag gtataagtta tatattaaaa aaataagtat aagttatact
     1321 ttaaatacaa ataatgtata acttatactt taaataataa ttttataatg ttgatttaat
     1381 cgcaaattta cacgttgttt attaagttat actttaaata tttaatgtat aacttatact
     1441 ttaatattca tttatctagt ataacttata ctttaattga ataccgcatg aaaaaaaatt
     1501 attttggtaa tttttcaaac tcttcttgta tttcttctct tatgtgttct tcttttgcta
     1561 ctcctagtct atgctggata tatgagtctg ctaatgtatt aacaattttt tcgaaagaaa
     1621 ttctcccttt ttcactttgt aactccgtca tatagtcttg taaaattgag agtttgctat
     1681 ttaatgccgg agagatcttt ttaggaactg ataccttgtt tcttttatca ggaattgaaa
     1741 ctggtcggcc tctttttttc aatgtaatat tttttgtgtt actcaatgca ctattgctaa
     1801 agttatattc accgcttatt ttcacatctt ggcgtttgtt attgggtaat aatggctcaa
     1861 attcgccatt ctccatcatt ttaggaatat caaaatcttt aggcattact tatgcaactc
     1921 cttattattt atttaaaaac tcttgtctag catctagttc tgccaaaata tttataaata
     1981 cttggtgtgc tttataatcc cattgatcaa aatttccatt tttgtatttt cttaaatgta
     2041 taccaatttt agaatacctt ctcaatcttt tttgataatg aatgagattt ttggatacca
     2101 cggttccgta tttttcaatt gcttcttcta ggatttcttc atcaaaatca tcatttggtt
     2161 ctaccataca aggtacaata tctataattt gcaaatcaag atcgtatcta tcgaccatga
     2221 atttttgata atttacatat ttgttgactc cctctaagga ttcttcagct gtttgaaatg
     2281 aaattatcga atagtctgaa gctgccatag cattatcgga aaattcgcta atggttggtg
     2341 gtacatcaat aaatatttct tgataattgt tctttattgg aagaagtaat tttttcaaga
     2401 cagtaacttg gtctaattca ttttcatgtt tattagcaaa agcggagaat gatcgaaaac
     2461 tagtgtcaca tgccagaagg tcaagatttt catttacctt aataatacta cttgctaatt
     2521 ctccatttgc gacaccattt gttatagtgg ttttgatatc ggttataccg aaagttcttg
     2581 ctatgacttg tgttaagttc gcttgagggt ctaaatcaat aataagcgtt tttctatttc
     2641 ttatcattgc attgtcataa gcaagcatgc tgactatctt agttttacct actcctcctt
     2701 taaaattgcc aactgtaaat gtagtccctt gaaaagtatc catgtaaatc ctcccaatct
     2761 ttaattgata attaaagtat aacttatata tcggaaatgg tcaacattaa aagtataact
     2821 taaataataa tatctaaaaa cgtttggata tagcggtttt taagaataaa agtataagtt
     2881 ataccctaaa tatatatggt ataacttata ctttaattat ttaaggtata acttatacct
     2941 tagatattgc ttttcgaaaa tagctattct agtctatctg tttctttact tttcgctgct
     3001 gatttgaagt gaattgatca tattcatttt ctagtattaa taacttttcg tatacatcgg
     3061 tcatattcat actagaagca agacttagat catatacaaa tgaagcaggt aatgatttta
     3121 ttctacgttg tcgggttatc cagcttgcaa ccgttccttg tgggtaggca tataactcac
     3181 aaaattgttc tacagtgatt cctaaatatt tgataatgta aacattgatc gggtgcgagt
     3241 aaacaaatgt attgatggac atggtgcgta agcctacttt cttttattat tgcgtttgca
     3301 ataatagttt aacacacgca ttattctagt atctactcct atttgtaaaa acaaaaaagg
     3361 attcatagag ataactctat agaatccgta aaatacctta tataaataac gggaaaatat
     3421 aaaacctaaa attatgataa cctgctgcag cttctacata gaaagagaaa cgctcaacac
     3481 caacctcatt tatcaaacgt tggccatacc cccaaatctc ttctgatagc tgatcaaagg
     3541 ttatatactt tagctcatat tgctggataa tgttcattct aatcgactcc atttattatc
     3601 ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt ttgtctttgc caacgtagat
     3661 tcccagctca tcactaccta agatctttcc aacaatatcg tctggatagt atccttcagc
     3721 gtttcgttct tctttttgaa tacatactga tctatttttt atgactgaag cgttcaatat
     3781 ttccgcaatc tcttcagtcc tcatttgttc ctttgctaaa ttttgatttg cacgttgctc
     3841 attatctgca tcgatctctt cagtatgatc cgataaatag aaacctgccc atttcaatcg
     3901 tcctcgatcg ttatagtcaa tcatttttaa gcccccctgc gccgcctgca tggccaccaa
     3961 ccaacgtgct tctagtaata ctacgagcgc cttctaacat cgaagaagca tgtacaattg
     4021 acgtaaaacc atacttctgc ctaattttat ctattatttt gtctagtttt tcgtcatcaa
     4081 tctgtttttg tggatcacta aacaaatcca gctgaagcga atcagtataa actagcttgc
     4141 tgtaagtgat tccgacatgg cggacttctt gtccctcata gtatttatta aataagaaca
     4201 gacaatagtc ttgtagctct cttgtattat ctgtgataga tatcttcatc tggtgtgaaa
     4261 accctggtct cgtttctaaa atcgacgttc caatgtgtaa atgaacacaa cctgttttac
     4321 agtggtgttg cctaatacgg attgctactt gctcagccat ttcttttacg accagttcga
     4381 tttcatttct tctagtataa tctcgtggta gcacttgtga attgccatat gatttttcct
     4441 ttgtcggttc tggtgcgatt gctatgtcag ttcgatcaat accatttgca tggaagtaca
     4501 gctgtaaccc aatcacacct aaacggctct taatggtgta tggattccaa ttagctaaat
     4561 ctttgatact catgatcccc atttggttga gccttttttt cattcgagaa ccaatacccc
     4621 aaaagtctgt catttcagga atattccata ctttatcagg aacattttga tacgtccatt
     4681 cggcaataaa tccttcatta tgcttcgctt cattgtctaa ggctagttta gccaaaagag
     4741 gattatcgcc aacacctact gtagcaatca atcctagctc ttctttaata cgttcttgga
     4801 tcatttgagc gagattcttc cttcgctgac ttcgtgttcc ttcagtcgta aaaagattca
     4861 gtgatcgggt cacttttaag attgattcat cgatcgagta aatcagtaga tcttcatcag
     4921 ccacatatct tctgaaaata ttatttacct gcatattacg cttaatataa aggttcatac
     4981 gtggtggaac aacatgtaat gttttaggga aaggctgtgg tagatcccgt ggtctgctga
     5041 cattagaaat accatatttc ttctttgcag ctggtgaaga agctaaaatc aaccctgatc
     5101 ctgtattatc cgctcgactc atgacaacta attcagtcgt taatggatct aaatttcttt
     5161 ctatacattc gacacttgcg taaaaacttt tcatatcgat tagaaagtag tcattgactg
     5221 gctcttttga gtaatcaagc ataaagttct ccccctcttc tctcatatta tacaaacata
     5281 cgttcgtgtt tgcaacgagg atcttctcta aatagaaaaa tagccagaca attgtctgac
     5341 tataataaac ctatttgttt ttttcgttta gcaattggaa gtcttgatca atgaattggt
     5401 aacaattccc acaattttta caattgtatt gtttcgtttg aatattgagt tgaatcaact
     5461 caccacaagc acagtcaatc gatacagccg ttcttcgttc taatccgtat aatggacgtc
     5521 cccaaagagt gtagcctaat tgttgttctt tcatttactt cgttcctccc taaaaatatg
     5581 tctattcgta gctggtatga cgcttcgttc gaccaatgat acatcttaat gagtgaacct
     5641 gaccttagaa tgctcgtata aacaacgatt tatttgattt aaaagagatt tttatattaa
     5701 ttttatcaat cttctactca ctcgcttcta tttgtttctt aattctaaaa agtgtactac
     5761 gactgaaacc cgttatggct tccacttctt tatacgattt gccggaagta atcaattcat
     5821 atgcatgcct cttctttggt gtgattgttg ctttcggacg tccctcttta aaattaggat
     5881 tattcttttt cgcaaataat ttcccttcct gagtacgttc tacaatcata tcacgttcca
     5941 tttcagctac actcaacaac gtacgtgtaa ccattcgacc catcgatgta ttttcgatcg
     6001 tccctagatt caatacctta atcgtgacgt tatcatcgag taaaggttca atgatctcta
     6061 gtgcttctct tgtattacgg gcgaatcgat caagtttcgt aacaacgatt tcgtctccag
     6121 gcttcagacc agccaacagc tgattaaact ttggccgatc tcgtttcgta cctgtaaatt
     6181 tttcagaaaa aatattcttc ttctcaacac cgttctcaag tagctgttgt atctgttcct
     6241 ttgtactttg agaagcggtt gagacacgtg catagccata ttttgtcatt taactcacct
     6301 gcttttacaa atcatctatc gtatactcag agtatccttc atagaagtag ctgacatcga
     6361 ttcctttcat atataactca cgatcgtcaa ttttatcagt cagggcattg gaaatgagat
     6421 agcgtatttc caagtcattt attggactgc gctccattgc agaaagatag tctgctttat
     6481 cgatcaagtt ccaatcgacc actttttgca attcttcctt taaaatgaga tccagccaaa
     6541 ttctagtact gcgcccgttt ccttcgcgaa aagggtgagc gatattcatt tccacgtatt
     6601 tcttaataat ctcttcgaaa gaagtttgcg gcatctgatc aatatgattt aaagattgtt
     6661 ctaggtacat gaccggcgca aaacgaaaat ttccttttgc aatattaact gtccgaattt
     6721 ttcctgcaaa atcataaatt tctgaaaaga gaaaatcatg aatttcagac aagcctttaa
     6781 acgtgccgac ttcgatttca tttatttttc cagaatcata taattctttt gctttttgct
     6841 tactgagttt ttcttcagcc ttggccaact caacctgatt cgttaaaccc aatttatttt
     6901 ctaaaatcat ggaaaatccc cttaactttt tgacataagt ttatgaaaca ctacaaatcc
     6961 tattgtatcg cgaatttaac catgtgtcaa aaactattaa gtttttgaca cgtagttagt
     7021 tttcaataga agccatctgt ccttttcttt ttaaatggca aaaatttttt attttattcg
     7081 gcacaaacta taaacattta atcgctaaga aaatcactca attattttga gtgatttttt
     7141 cagttcttat tttaaaacaa agtgttcata aaatcaggtg tttctttatt tttcagtagc
     7201 ataccctgtt tttattttga gtattagtaa taattaaaaa aaccacttgt aattttatat
     7261 ttgattaatt ataataaaag gtgcttcggt agcaatttat gattattaga tttttgattt
     7321 ttttattttt ctgtttgtgt ttgtgatgaa acatctcctt ttttaaaggc ttggttttca
     7381 ttactgcata agagctaaca atggtactgt tagctctttt tttgttcttt tctctttttc
     7441 tatcccttaa cgtttattct tctttttact atataaaagt gctatcactt ttatttttta
     7501 agtcaaaaag tgacgtcact ttttgagcgc tggtatgttg aatcctgctt tttcctgcgc
     7561 aatcaataaa tctaacgcaa tgcacataat cctggattca cttttaccgt atttctgttt
     7621 aagttgctgt aattcttttt gtctcttttc atccaacgta acgggtaacc gtttatttcc
     7681 tattgcaatt ggcattgata ctctctcctc tcatcgaaat aatcctatga aaaatatggc
     7741 tgcaatcatc ttgaatagta aactcacgag ggctaataca aaccgccaaa aggtcaaaca
     7801 aaacaccagt attaccaaaa atactacgaa gtttaaatgc acagttgcag catgacgcat
     7861 aagcatttcg ccaacaaata gtactaataa taagggaata atctttttca tataaatctc
     7921 cttctttctc tttggttagc gggctttgct cgcttatttc gcaccaccta agcggcgggg
     7981 gcggttggag cgattggtgc gaaataagaa ccattccctt aatttcaagg ttttttgttc
     8041 ttatttcaca cattctcact tttcggtata aatgattatt gacctttttt cctttcaaat
     8101 cgttgatatg gcatttgttt acggtcaagc tcattttgga tttcttctgt atcgtcgtaa
     8161 gtgtaggtgt ctaaaaaatc agttaaagtc gtgtaccaat catcccctgg gttatctact
     8221 tcttctacat attccctcaa aaacgcttcg ggtacagcga gttcgtaatc tattcccatt
     8281 agcacgatag attccgttcc acacgttaaa aatctagtaa atacaaggta ataatcgtct
     8341 accgtcgcat ctttactgtt taactcttcc atttcttctt ccattaattc catgctctca
     8401 aaaattgaat aatattctga ttctgagtaa tagtgtttcc tgcaaatcgt gtagctaata
     8461 aaattttcga ccaactcact tttttctagg taaacattca tgacgtattt ttctgtttct
     8521 agtcgtagaa gctccgctcc tttttttaat gattcaggac gacgtttata cacatctaaa
     8581 cgattattgc tgactgtgac ctttgtttga ctcgtttcgc tttccaaata taactgttct
     8641 tcaacgtttt gaacaaagta cttcatttct tccatacaac taaagtcttt ttgaatccgt
     8701 ctgattactt cattcaaatg ttctttgttt gttaaaaaac gagaaatgtc ttccattgtt
     8761 agttgataaa ctacattatt cattttcgtt gtcctccttt ttcatggtgt aataggcgcc
     8821 atacttattg atattttcaa aataatctaa ccattcactt gaaacagtct tggctttttt
     8881 cgtttcgata aatttcgcat acgttagttc taaagcttgg tattcagaag gtttaatagt
     8941 gcttggtttg acacctactt ccaagcatag taaggcttgg aaaaaccatt ttgcattttg
     9001 ctttttcagt tcttccagtg ttcgatagta cgtcgcaata agattcattt cgacacctga
     9061 ttcaatcatt tcttccacag ctaccgacaa tttttcttta taatgttttt ttggaacacg
     9121 tgtttcgttg tataggaacg tcatatcctc cattttttgt gccattttat ccaaaggtaa
     9181 tttgacaaat tcgtcatagt aattttgagc catcatttta t
//
LOCUS       DACEKQ010000103         9021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEKQ010000103 DACEKQ010000000
VERSION     DACEKQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.108"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..1517)
                     /locus_tag="IW671_002371"
     CDS             complement(51..1517)
                     /locus_tag="IW671_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0899341.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IW671_002372"
     CDS             1904..2500
                     /locus_tag="IW671_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0899342.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IW671_002373"
     CDS             complement(2558..3040)
                     /locus_tag="IW671_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899343.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IW671_002374"
     CDS             complement(3146..3343)
                     /locus_tag="IW671_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899344.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IW671_002375"
     CDS             3638..4396
                     /locus_tag="IW671_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0899345.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IW671_002376"
     CDS             4456..4884
                     /locus_tag="IW671_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899346.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IW671_002377"
     tRNA            4930..5002
                     /locus_tag="IW671_002377"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IW671_002378"
     CDS             complement(5152..5523)
                     /locus_tag="IW671_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0899347.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IW671_002379"
     CDS             5738..5923
                     /locus_tag="IW671_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899348.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IW671_002380"
     CDS             complement(6006..6194)
                     /locus_tag="IW671_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899349.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IW671_002381"
     CDS             complement(6218..6481)
                     /locus_tag="IW671_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899350.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IW671_002382"
     CDS             6639..6896
                     /locus_tag="IW671_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0899351.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IW671_002383"
     CDS             complement(6967..7158)
                     /locus_tag="IW671_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899352.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IW671_002384"
     CDS             complement(7299..7619)
                     /locus_tag="IW671_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899353.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IW671_002385"
     CDS             complement(7642..8076)
                     /locus_tag="IW671_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0899354.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>9021
                     /locus_tag="IW671_002386"
     CDS             8411..>9021
                     /locus_tag="IW671_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0899355.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEA"
BASE COUNT         2872 a         1799 c         1448 g         2902 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatttactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa caacctgttg tacaagaaac ggttcaaacg gaaaaacaag cagcgcctgt
     8761 tgctgaaaca caacctgctc ctgctgtaac agaaactgct gctactccag caagcacaag
     8821 ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt tcttacacag caacgaacgg
     8881 acgttacatc ggacgttacc aattagattc ttcttactta aatggtgact actctgctgc
     8941 aaaccaagaa agagtagcag aacaatatgt tacttcacgt tatggttctt gggaagcggc
     9001 gaaagcattc tgggaagcaa a
//
LOCUS       DACEKQ010000104         8969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEKQ010000104 DACEKQ010000000
VERSION     DACEKQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.109"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW671_002387"
     CDS             complement(57..350)
                     /locus_tag="IW671_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0899356.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW671_002388"
     CDS             complement(378..1043)
                     /locus_tag="IW671_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0899357.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW671_002389"
     CDS             complement(1183..1815)
                     /locus_tag="IW671_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899358.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW671_002390"
     CDS             complement(1822..2889)
                     /locus_tag="IW671_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0899359.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW671_002391"
     CDS             complement(2886..3617)
                     /locus_tag="IW671_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0899360.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW671_002392"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW671_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0899361.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW671_002393"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW671_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0899362.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="IW671_002394"
     CDS             5780..6949
                     /locus_tag="IW671_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0899363.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8838)
                     /locus_tag="IW671_002395"
     CDS             complement(7216..8838)
                     /locus_tag="IW671_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899364.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgttt
//
LOCUS       DACEKQ010000105         8747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEKQ010000105 DACEKQ010000000
VERSION     DACEKQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.110"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            276..458
                     /locus_tag="IW671_002396"
     CDS             276..458
                     /locus_tag="IW671_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899365.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            442..627
                     /locus_tag="IW671_002397"
     CDS             442..627
                     /locus_tag="IW671_002397"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0899366.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            649..774
                     /locus_tag="IW671_002398"
     CDS             649..774
                     /locus_tag="IW671_002398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0899367.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            870..2390
                     /locus_tag="IW671_002399"
     CDS             870..2390
                     /locus_tag="IW671_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0899368.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2577..3149)
                     /locus_tag="IW671_002400"
     CDS             complement(2577..3149)
                     /locus_tag="IW671_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899369.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW671_002401"
     CDS             complement(3156..3818)
                     /gene="rpe"
                     /locus_tag="IW671_002401"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0899370.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3834..4349)
                     /locus_tag="IW671_002402"
     CDS             complement(3834..4349)
                     /locus_tag="IW671_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0899371.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4351..4635)
                     /locus_tag="IW671_002403"
     CDS             complement(4351..4635)
                     /locus_tag="IW671_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0899372.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4669..5997)
                     /locus_tag="IW671_002404"
     CDS             complement(4669..5997)
                     /locus_tag="IW671_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0899373.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6011..6466)
                     /locus_tag="IW671_002405"
     CDS             complement(6011..6466)
                     /locus_tag="IW671_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0899374.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6469..8415)
                     /locus_tag="IW671_002406"
     CDS             complement(6469..8415)
                     /locus_tag="IW671_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0899375.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2917 a         1625 c         1372 g         2833 t
ORIGIN      
        1 aaagattcga ttaaattagt tgagtaaatg cttcgtacga acatagttga tttattcacc
       61 atacttccca ttacatcgta aaacgctata tttaagattt caaacaacta tattaatagg
      121 aagaaaactt catttttcct cttagttcta taaaaagtgt ggctaatttg aggcaaaata
      181 ttaaattaaa tgtaagcgtg tcataggata gtatctcgta cgccgattat ctttcttcta
      241 taattaaact atcaaatata gaaggagaaa aacgaatgaa taaaaaagat attgtaccag
      301 ttcaattttc aagtgcggct caaaaatcgc aaattgacac caagccaaaa ctagcttgca
      361 gaatcaaatc ggaagataag gaaatattta tttataatgg aatggataaa tatcttttgt
      421 ataccttgtt aaaagagttg aatgccaatg ctcattgatg caactgcatt gaaacatatt
      481 tttatcgttt gtggaaaaac agatcttcga aaaggaattg atggactagc agctttcgtt
      541 atagaagaat atgaccttga tgtttacgat caagcgcttt tcttattttg cggtacgaag
      601 aaagatcgct tcaaggctct ttactaggat caaaatggat ttgtattaat gtacaaaaga
      661 tttgaaagtg ggtaccttca atggccgcgt tccagaaaag aagtcttact gactggcctt
      721 tcaatcgaag aaaaatgtac gctaaaagaa acgaaaaaag gtgcgatata ttagacgaaa
      781 accttgttaa atcaaggatt ttgtctgttt ttcgttgtca taaaatggta taatagatgt
      841 aagaaaatcg ctagaatggt ggtgtaaaaa tgacaaaaat ggaatctgaa cttcttgtac
      901 aaatcaagca attaacacag ttgcttgaaa tccagcaaca ggaaaatact cttcttagag
      961 aacaaattgc tgaaatgaat cgacgcttgt ttggacgaaa aaaagaaaca ccacccgtag
     1021 atgggcaaat cgatttatta gacgactcaa cttttaatga accagagcac actggacaag
     1081 aaagccagga acccattacg gtatcttctt ttaaacgcag aaaacgcaag ggactcaaag
     1141 cactttcatt agaaggtctg cctgaagttc ctattcatta tgaattaaaa ggtgccgatt
     1201 gtttgtgtga agactgtggt catactttac atgatatcgg tgcgacaagg cttagagaag
     1261 agccattgta tgttcctgcc catatagaaa aacaagtcat ctaccaacat gcctatcatt
     1321 gcagagaatg tgaagcatta ggagaaacaa aaatcaaaaa agcgcctgtt cctaaaccgt
     1381 tgatcaataa tagtctaggt tcctcttcaa ttgtgacaca gagtatttgg gaaaaatttg
     1441 ttaaaaaggt gccggcatat cgacaagaaa aagaatggca tgcacttggt ttccctcttg
     1501 atcgtaaaaa aatcactaat tggcatatca aggtaagtga aatggtttta gagccaatct
     1561 acaattgcct tcatcaagaa ttaagcaatc aggaggtctt acacgcggat gagacaagct
     1621 atcgggtatt gaatactcat aaagaaaaga ccttttactg gttatatgct tcaggtaaaa
     1681 gcgaagaaaa acaagttgtg ctctacgagc acgcaaattc gcgttccgtt gatgagccaa
     1741 aaagtttttt agaatcattt agcggtttct tgcatactga tggttacgcg gcctatggaa
     1801 agctgccggt aaacaatgtt tattgttggg cgcatgttcg cagaaagttt tttgaagcat
     1861 tgggtaaaca accgtcccca aaaagtcatg ccaaaatagg attagacttc tgtgacaaga
     1921 tgtttcgaat cgaacggacc tttaataagc ttagcgcaga agaaaggttt gtagcgagga
     1981 aggaaacgtt gcagcctgtg atgactgaat tttttcattg gtgtgagagt tttgcttatt
     2041 tgccaaaatc gcagcttgga aaggcggttc aatacgcagt caatcataaa gacggacttc
     2101 agattgtgtt gcttgatgga cgcttggaac tttcaaataa ccgagcagag cgtgccatca
     2161 aagagttagt gatcggtcga aaaaactggc tattttctaa aagcttaaaa ggcgctagat
     2221 caaatggaat tatattgagt attatccaga cagctgttgc gaatgggtta aacatccgca
     2281 aatatttaaa tcatcttttt acagagattc ccaatctgtc ttcgatgaca cctgaagcgc
     2341 tacgcgctta tttgccatgg aatcagcaga tacaagaaat ctgtaaataa tataagccac
     2401 aaataacctg atttgagtgt atcaaatcgg gctatttgtg tttaggtacc attctatgac
     2461 acgcttacgc tcaaaactac tgaattatta agttatgtct aatcaaataa aaaaaccatt
     2521 ctcatatttg ctgaataatt agcatttaga gaatggtttt ttatctttaa aatctactac
     2581 tcaaaatctg catgtcgagc aaacatcgtt tctgagcttt cacctgtttt atccattaaa
     2641 gataacacta aactatcaaa ggtgagaaat gctaattgtt caaaacttga acccataggt
     2701 tgtgaaaaag tatcttcttc tcgttgattt tcatctttgg taatacctgg taatacaaga
     2761 acagaatctg ctaattgacc aatcgacgag tcttttttca tggtaactaa agcaatgctt
     2821 acaccacttt gatttgcttt ggccgctaaa ttttttaaac tacctgtttc acctgagcca
     2881 gaacagataa ttaataaatc tccaggttgc gaatgtggtg aactaatttc accgacaagg
     2941 ctgactgaaa aacctaaatg cattaagcga ttcgtaaatg cttgcatggc aattccagag
     3001 cgtcctgcac cagctagaaa gatgtgttta gcttgaacaa ttttctcact aaattgttct
     3061 aactctacag aattaatacg ttttgagtta ttttgtaatt ctaagataat cgcttctaaa
     3121 tagtttttat tttggtcttt attggtcatg atcttttact tcacttcctc taatgattga
     3181 tttagtttat caatttgttc ttgcggggta tttttcccat tgaagatgaa accgcctgat
     3241 acaaaaatat ccgcccctgc tttagaacaa actttaatcg tttgattatc aatcttgcca
     3301 tctacttcaa tatcatacgt ataatcattt tgtttgcgta ttacatctaa ctgttcgatt
     3361 ttaactactg tctcttgaat aaaatgctta ttctctgttc ctggattgat tgtcattact
     3421 aatacttgat cgactaaaga taatactggt tcaataaaac tgactggtgt tgcaggatta
     3481 aaagtaatcc ctacttttgc tcctgcttcc ttgactaact gaatgacctt cattgcgtgt
     3541 ttggtagctt caatgtggaa ggtaacaata tctgctcctg cttctacaaa tcttgtgaca
     3601 attttttctg gtgtatcaat catcaaatga acatcaaaca caacgtctgt tagtggacga
     3661 atatctttaa taacacttgg tccaaaagcc atatctggta caaaacagcc atccattaca
     3721 tcaatatgca aataatcaac attcttttct tttaagattt caaataactc tttaacttct
     3781 gcgataggtg catttaacaa tgatggagca attgtcattc tctattcccc cgtttattct
     3841 cccataatca tgagattgat tgttctagtt cgctctctta gttgatccca atcgacaaaa
     3901 tagacacggc ccatttctcc taataaaagt ttaccttctt gaacaattaa agtcattgag
     3961 ttggttccat aaatagatga acgtaaatgt gcatcggtat ttagcatgac ccatttttct
     4021 gcagggtaat tgggatcaga taatcttgtt ccaaaatcaa tgtgttttgg ccccggactg
     4081 ttgtattgat tttcactcgt catttttgga ataatcgttt ccattacatt gttgatgtct
     4141 acatgtaaat aatcatcacc aaaaaagttt gtgtcatgca tcgtttcttc aaaaaagagt
     4201 gagcaagttg tatgcgggct cgaaattact aacattccat tctcaatacc tgctgtatca
     4261 actgtatcta aaagttcttt tcgaatatca tgatacgaag gacgccctga aactgtttca
     4321 atagtcaaag tgtcatgatg aaaacgcata ttaaacacct aatctttctt ttacatctgc
     4381 ataaatggca tctgtaccaa tacctgttaa taagcccaag ccattcgtta ctttagcttt
     4441 aattacatca gggacaattg ttgtacttac tacagcatca tattcatcaa ttttatttag
     4501 ctcatcagca attttagatt gataaatttt cacttgatct gttaatcctt cttcttccaa
     4561 ccattttgat aattttgcta ctgcaattgt agaagtagca tgacctgctc cacaaataac
     4621 taataatttt ttcatattga tctcttcttt cttattttaa ttttatgatt aagctttctt
     4681 cactttgttc atgtagatag aagcacctac taaaacaact cctaatccac ctattccaat
     4741 tacaccaaag taagatgcta ataaaacaaa caaccctact ggccataatc cacataacaa
     4801 catagttact aatcctgctg tccccacatc gaaacttgct aatgtaaaca catcagtgat
     4861 aattggtgcc aaccatgttg ccatatataa tgttgtaaca acatacaacg aacatcctac
     4921 taatgatctc acaatatttc ctttaaaata aggaatcatt aaacagatgg cgaaagcaaa
     4981 gaatgctaaa tctccaaaag gtaatacttt atttcctgga agaattagtg ctagcaataa
     5041 tgagattggt acaagcaata gagaagttga cataatagtt ggatgaccca cagttaacgc
     5101 tgcatccatt ccaatgttta cttggcgacc atttaacttt ttattcgtaa attctttcgc
     5161 cgcttctgca attggcatta aaccttccat aaacattgct accattttag gcataatctt
     5221 catcactgcc gcagtcataa ttcctaagtt tccaattcct gctacgtcat agcctgccat
     5281 aatacccact acaataccga taattaggcc aataacaact gtgtcaccta aaacaccgaa
     5341 acgttttgtt aatgtttctg catctgcatc tattttatta aatccaggaa tacgatcaaa
     5401 aacccaattt actggtttgg caaatagtac tcctgataat cccatcatat gtgtgataga
     5461 gattcctgga tagccgaaaa attcgctaac agttttttgt gtacgatctg ctaagaacca
     5521 ttgtaaaatt actgcgacaa tcgcgccaat taagccaaac caaaagtttc ccgtaacaga
     5581 ttgagtaatc atacctagga aaattgcgaa ccaaaagttc caaatatcaa cattcaatgt
     5641 ttttgtccaa cccaaaacca caaatatgaa gttaagaatt aatgaaattg gaatcattaa
     5701 aattcccaat gatgtagagt aagccaaagg tccaccaaca cctacgccaa tatctaggat
     5761 attcaggcca ccaccgtatt gttcccccat ttgttgaatt gctgttccta aaccatctga
     5821 taataaactt aaaactaatc ctaaaccaat agaaccgata ccaacagtaa cgccggaaac
     5881 aaatgcttta ccaggtttta atccaaataa tatcccgatt acaaaaataa tcagtggtaa
     5941 aaagacgttt gaccctaatc caataaacca ttcaaaggca tttaatacaa tatccatctc
     6001 tctattcctc ctaataaact cctgcagaat gtactaactc aataaatgct tctttcgtgg
     6061 tacatgcaag taattcattt actttttctt cattttgaaa taggctaact aagttttgta
     6121 gtgtatcaat ttgttcatga ggttgtgaca ttgccaacat aaagactaaa cgtacaggta
     6181 tttcatgaga tttatctgtc atatctgaaa ataccagtgg ttcctttaat gaggcaaaac
     6241 agatttgaga gctatttaca tgtgcggaat ctgtatgtgg gatagcaaac ccaatttgat
     6301 ttacttctaa tccagttgga aattcttgtt cacggttggt gataccttct aagaaactat
     6361 ctttcacgca accagcgttt tttaattgtt cagacattac ttgaaacaat tctttttgat
     6421 ctttcacttc taattgaaat aaacataatt ctttatctag atacattttt attctccttt
     6481 ttgttgaaaa attcttagta atttttcgct atcatgaact tgtaacaagc gatcgaccat
     6541 ttctttttgc ccaataaatt ggtaaagttc ttcatatact cctctaatct cagatacatc
     6601 ttcttcagct aaactaacca taatgactaa tttgactttt cgatcgcccc aattaatcgc
     6661 attttttaat gctacaataa aaatgttgga ttcaatgacc attgatggat tcgcatgcgg
     6721 taaagcaaca ccactttcta aattagtatt ccccattttc tctctagaga agatagaagc
     6781 acgaaactct tcagtaacta atgaattggc ttctagttcg gaaatcatca aatccaaaca
     6841 ttcttctttg ctttccacct gtactcctgt ttttactaaa gatgattgaa caaatttaga
     6901 tatggtggga actttaaccg ctttaggaat cagttcattt tgaataaatc gcgaaggttc
     6961 ttctattaaa tatttagtat aagcttccat aatattcata taatctgttt tacttaccaa
     7021 tggagatacc cttactaagg gtataccacc tgcatcaaag gaaagagagg aaataatcaa
     7081 atcaacttcc ccaatttcag ctaaatgaaa cttatctaaa gttgatattt ctacattatc
     7141 ttttgctggt aaaaaccgtc gaactttatt gtaaattaat tgcgcagaag aaataccgta
     7201 ttggcaaata atgatgatat tatttgcttt ggactgatct tcaatagcaa tctgaaaatg
     7261 aatcaaaatt aaggaaactt catcgtccgt taaagtcaca tcaaattctt cttcaaggat
     7321 ggacatggcg taccatacta tgctaaagag ttctgaatat tgagatttga tattttctaa
     7381 taacggattt tgaattttaa tacctttttt taaacgaagg accattgctg gcacatgata
     7441 caataaggat tgatataatc gatcattttg agtcaaatca attttttcaa tctcactcat
     7501 tctttcaatc aatttatgaa cgctcatagc gtactcttca ttgctggttt ttaaattatt
     7561 tgtcacacga tgtgcaaata gttgacgaca tagatattct ttatctgcgt cataaaatgg
     7621 aagattcaat tttgccttca attcttcaat taaaccattt gccacaatgt acgtttccat
     7681 atagcgaatg ctattgaaga ggaattcttc ttcaacagtt aaatgatgac ctttcagtaa
     7741 acggttacat tggataacta aagtagaaat caatgaccga acatagtaat cagaaacctt
     7801 ttcagtcagt tcactgtaat cattgaatag caaatcatga atgacactaa taacttttga
     7861 atcaaacaac atatttaaaa cttgaaaaaa tgtttttgat tccatgtcat tcgcataata
     7921 gaatacaagt tgcttgattg ctcgttgaat tgcttgttca ttaccagtaa aactaattcc
     7981 ttctgcagat gactgcaact taacttctcc actgccaata attttattga taattctcag
     8041 gtcattgtat aacgatgttt tactaaccat aaaactttga gacaaatgag ttaaagaaac
     8101 aggcttatca ccaaacaaaa cttctttaat gatctcgaca cgtctttttt ctggagaata
     8161 aatatctttt tcaagataat tttctaccga tgaaatcaaa tgttcaacgt ttttcctatc
     8221 acctatgagt ttgataccca aagaaggtaa tcgctcaatt gttacttggt aattaactac
     8281 ataatcttcc aacaattcaa tatcttgata aatggtcttg gtcgacactg ccaatttatc
     8341 actaaaaaaa gaagcgggtt taaagtcttc ttgtcgactt aataatttta ataaatttac
     8401 atgtctttca atcaaacttg ttcacctctt aacgctagca tttgttacct cttttcatta
     8461 tttattctag cactggaacc cccttccctc aattacatct atgtcaataa tacttgtgga
     8521 agtttgttgt aaaggaaatc caaataatct taaaaaatga gaaacaaaaa aggagaaatc
     8581 cttaaaagag ctaagatatt ccaatagaaa atactcctta ttatggaagt aatatgaaat
     8641 attacaattg aaaaataaaa acaatataat ggcgtcacta aaattcgtat ttttttatta
     8701 taatataact taattacaaa tgctcatcta tgaacaattg agtccta
//
LOCUS       DACEKQ010000106         8570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEKQ010000106 DACEKQ010000000
VERSION     DACEKQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.111"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(306..836)
                     /locus_tag="IW671_002407"
     CDS             complement(306..836)
                     /locus_tag="IW671_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0899376.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1018..1674)
                     /locus_tag="IW671_002408"
     CDS             complement(1018..1674)
                     /locus_tag="IW671_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0899377.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="IW671_002409"
     CDS             complement(1801..2274)
                     /gene="dfrF"
                     /locus_tag="IW671_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0899378.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2693..2911)
                     /locus_tag="IW671_002410"
                     /pseudo
     CDS             complement(2693..2911)
                     /locus_tag="IW671_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2942..4156)
                     /locus_tag="IW671_002411"
     CDS             complement(2942..4156)
                     /locus_tag="IW671_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0899379.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4497..4739)
                     /locus_tag="IW671_002412"
     CDS             complement(4497..4739)
                     /locus_tag="IW671_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0899380.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4771..5328)
                     /locus_tag="IW671_002413"
     CDS             complement(4771..5328)
                     /locus_tag="IW671_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0899381.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5341..5529)
                     /locus_tag="IW671_002414"
     CDS             complement(5341..5529)
                     /locus_tag="IW671_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0899382.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5542..6108)
                     /gene="amaP"
                     /locus_tag="IW671_002415"
     CDS             complement(5542..6108)
                     /gene="amaP"
                     /locus_tag="IW671_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0899383.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7338..7811
                     /locus_tag="IW671_002416"
     CDS             7338..7811
                     /locus_tag="IW671_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0899384.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7820..8047
                     /locus_tag="IW671_002417"
     CDS             7820..8047
                     /locus_tag="IW671_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899385.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1684 c         1274 g         3005 t
ORIGIN      
        1 gaaatcaact gtctttctca tatttactat tcaattttca tagtgatata ggtacaattt
       61 atatcactac tgtaaaaatg gggttttgcc ttataaatac agcgttttcc gcttgttagt
      121 gtatagtgtt tcttgttttt tgtgcccccc ttgttagata acgggggctt tgaacggctc
      181 gcaagctcgc cgaaccgccg aagcgaaccg cccccttgca agcatggcgg aaaaatctta
      241 aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt tctgtttctc
      301 tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg tgttttacaa
      361 agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt tcctaacaga
      421 gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa ctgctgaaca
      481 agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag ccgttccaat
      541 aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca ctctttatgt
      601 gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag ttcagtaaca
      661 acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt aaattctgta
      721 taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac tccaccatta
      781 ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt tctcattttt
      841 tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag ggaacaactg
      901 attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa atcactaacc
      961 atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa tttttgccta
     1021 tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc aatgtcttga
     1081 aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa tcagccaaat
     1141 tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc tttctttcgg
     1201 tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag tgctttccca
     1261 tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa agtctgcaat
     1321 ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac gctcttgata
     1381 attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa ctatatcttc
     1441 aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac catcagcgtt
     1501 tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag atactaaggt
     1561 ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat aagtatcttt
     1621 cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat tcatattcaa
     1681 tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct tacaacaatt
     1741 tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa tttactcaat
     1801 tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa gtgtttcccc
     1861 tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc catcttcaat
     1921 atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag
     1981 tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa
     2041 tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt
     2101 cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac
     2161 attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt
     2221 accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt
     2281 catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa
     2341 ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta
     2401 tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc
     2461 tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata
     2521 gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca
     2581 aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt
     2641 ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt
     2701 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
     2761 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
     2821 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
     2881 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
     2941 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
     3001 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
     3061 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
     3121 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
     3181 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
     3241 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
     3301 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
     3361 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
     3421 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
     3481 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
     3541 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
     3601 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
     3661 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
     3721 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
     3781 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
     3841 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
     3901 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
     3961 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
     4021 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
     4081 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
     4141 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
     4201 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
     4261 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
     4321 aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt taaagatatt
     4381 ttttcataag gaataaagaa tacttcatat aaaatatata aaaaagaacc gcagcgagtg
     4441 tcgctacggt tctcaaattt gttcttcatt tcaacataag tttttcttca atttgcttat
     4501 aatttacgtg caaagaatga aacgactaat actaaaatga ttgcaccgat caatgatgga
     4561 atgatagcca ttcctgcaat tgttggtccc caagttccta gtaaagcttg tccaattgct
     4621 gaacctacta aaccagcaat gatgtttgcg atgatcccca ttgatgatcc tttattcgtg
     4681 attgctcctg caattgctcc aatgataccg ccgacgatta atgcccaaat aaatcccatt
     4741 ttaaattcct cctaactgac ttcttacttc ttattgtaca cgtggttcgt tattttcttt
     4801 gactttttgt gcacctttgc ctacagcaga ttttgcttta tctgtttggc gtgaagcaaa
     4861 ttgtcctgtg gattttgctg cttctgttac tttgtcttgt actgtttctt gatctttttg
     4921 gtattcttct tgtgttttga tgtctgcaac attcacattc acttcgataa catctaaatg
     4981 tgtcatattg tttacttcag tactgatcaa ttctttgatt tggttgtaaa tatcttcgat
     5041 gtctttaccg tattctacga tcacatctag atccacagct acttgtttct tacctacttc
     5101 tgtattgata cctgctgtta cgttatctgt gttgactaat ttttcagcca tatttgagaa
     5161 aaatccgcca tctaatgtca atagaccatc tacatctttc aatgcataac cgatgatttt
     5221 ttggatgact ttgtcttcat aagtcaattc accattcatc atgcggtttt ctcctttttc
     5281 atttgccaaa actgatccat ttgttgtatt catgtttttg ttttccatct ttgtttcctc
     5341 ctatgattta aaaaattgat caactattcc tgactgtttc aaataaaaac cgattcctgc
     5401 gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa aatccaatcg ttaaccataa
     5461 aacagctagc agtagtccaa gacccccaaa gataataggg agcttgtatg ctgaaaatag
     5521 ttctttcatt atttggccct cctattccac tcgagagtgc ttgtagtctc ttgtatcttc
     5581 ttgttcttca taaccgctgt atgtgactgc cactttgacc gtttcttttg ttccgagtac
     5641 tttagttact tcttcttcga tatcttgcat caatacacct gttttcccta ctaatgaaga
     5701 agttcgtttc aagtctccgt tgacatggat atggattcga tttttcgttg aacgtatccg
     5761 tactttaggt gaatgaatga actcttcttc gtgtaaatgg gaacgtacca acccttcgat
     5821 cgcttttttg tcgattgtta gtttgccatc ttcccgtttc ataacgaaag ttcctcttga
     5881 ctttggatag aaaagaacga ctagcaagat gataatagca agaacggcaa atactacagc
     5941 tgcccagaaa atatattgtt gcatgtagta attcgttaaa gtaaagaagc gaaatcgttc
     6001 caattgttca ggcataatat acggcgcatt gatcaacgca atcgctagaa atactgaaag
     6061 tagcagtaaa ctcatgataa caccgattat ttttaacccc ttattcattt tccacctcct
     6121 ataaacttat ttttaccctg taaataactc taccgacaat cacagtatag atagcgaaca
     6181 gttagcatag catcagtaaa taaagcgcta ccaaacattg ctggatcata acggcgtttt
     6241 attgccagtc tgatactgaa ataacaattg cttacttatt ctctatttac ttttgattgc
     6301 tggctatact taaaggatat tattcaagat taaaaattgc aaagattatg ctcagttatt
     6361 attacaagat tctatttatt tattaaattt tttcggagaa ttaaaaaata caataaataa
     6421 ctttagttac tttaatgcgt tcttacttta tttttatatt atcatttttc ttattcactt
     6481 ctttggttta tactattctt ataaataaaa acgaggtatg tcctatgatg aaaagattgt
     6541 actattcatt gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat
     6601 ttggtatcag taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca
     6661 tcttcgtatt tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg
     6721 cacatgtttt aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct
     6781 tcttaatacc tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat
     6841 taagctattt cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat
     6901 aacattgtat aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga
     6961 aaaattctga tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta
     7021 cgtagttgtc ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata
     7081 aaaaatcctc caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa
     7141 gagacacaca tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct
     7201 gttaaatgca ctccacacct tcatactcta tcatttttta taaacgatat atttttattg
     7261 tttttcgata aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag
     7321 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
     7381 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
     7441 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
     7501 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
     7561 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
     7621 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
     7681 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
     7741 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
     7801 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
     7861 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
     7921 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
     7981 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
     8041 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
     8101 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
     8161 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
     8221 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
     8281 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     8341 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     8401 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     8461 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     8521 acagtgatgg acaattcttt tttattttta acattatttt gataataatg
//
LOCUS       DACEKQ010000107         8398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEKQ010000107 DACEKQ010000000
VERSION     DACEKQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.112"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            326..1519
                     /locus_tag="IW671_002418"
     CDS             326..1519
                     /locus_tag="IW671_002418"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0899386.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1649..3121
                     /locus_tag="IW671_002419"
     CDS             1649..3121
                     /locus_tag="IW671_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0899387.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3204..3992
                     /locus_tag="IW671_002420"
     CDS             3204..3992
                     /locus_tag="IW671_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0899388.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4059..4727)
                     /locus_tag="IW671_002421"
     CDS             complement(4059..4727)
                     /locus_tag="IW671_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0899389.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4842..6554
                     /locus_tag="IW671_002422"
     CDS             4842..6554
                     /locus_tag="IW671_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899390.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6557..8329
                     /locus_tag="IW671_002423"
     CDS             6557..8329
                     /locus_tag="IW671_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899391.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2565 a         1409 c         1847 g         2577 t
ORIGIN      
        1 aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgaatccc gtacgggtca
       61 tggaagatac tttttgagta tctttttctt tttatctgaa agaatgattt taaaaagctt
      121 agcagagtta tctttatgga tgattgtgct aagctttttt ggacagttct aatgtaaaat
      181 atttttatat tttttaagca aaaaaatgaa aaaatacgag cagaaaactg ttttatctgc
      241 tatacttggt tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg
      301 aatttaacta gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt
      361 ttctgaagga catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat
      421 tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt
      481 tttagtattt ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga
      541 aacaataaaa gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc
      601 tgtattggtt gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact
      661 tgagatccgg gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg
      721 tttaatgttt gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt
      781 gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt
      841 acgtccagat gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg
      901 cgtagataca atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg
      961 tcatgatgtc atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca
     1021 aacgaaatat tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc
     1081 cggattaaca ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg
     1141 cggtgctttt tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg
     1201 ttatattgct aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc
     1261 ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac
     1321 ggttccggaa gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg
     1381 aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca
     1441 ttttggtcgt acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa
     1501 agcaagttta gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc
     1561 agcttttttt atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga
     1621 tcctgtcata atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac
     1681 agtgagtatt ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc
     1741 tatgccaacg attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat
     1801 ttatttgctg acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg
     1861 acggaaacca atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg
     1921 tttatctcaa agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg
     1981 ggcaatgatg ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc
     2041 aaaagtttta ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc
     2101 gggaggattc atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat
     2161 cggaattcta ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga
     2221 gtctaaacca atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct
     2281 gttagggttc caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct
     2341 aggtagtatc gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt
     2401 catcatgatg catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt
     2461 ggctagtggt tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt
     2521 agggctgaat gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt
     2581 cggttctttc cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat
     2641 tggactacta atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa
     2701 ctatttatgg ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac
     2761 tacctcgact gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc
     2821 ttttaatacg ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt
     2881 gataaatggg gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat
     2941 catgaataaa ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc
     3001 attaagaaaa attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt
     3061 gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta
     3121 gtttcggtta agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac
     3181 ttcttactaa ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt
     3241 tagaaatgga agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca
     3301 ccagttcaga gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg
     3361 gcaatcatag tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat
     3421 tagctgtcat catgccatct actgatcttg gctggtacac agatacagtt tatgacatga
     3481 agtattggac attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat
     3541 taacaagaaa aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt
     3601 taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg
     3661 tttctgtagg aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag
     3721 ggatctttgg accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga
     3781 aagaactagt aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg
     3841 attttcttta tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg
     3901 tcacctttga acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac
     3961 gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta
     4021 tagagatcac aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt
     4081 ttattttgtt ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt
     4141 tcattcaatt ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact
     4201 acataattac gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt
     4261 tgtcgaagct gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga
     4321 gaggcaatgg cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc
     4381 atttgctcca aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct
     4441 gccccacaag ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt
     4501 tcattccaag gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt
     4561 ttttgcaaga gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga
     4621 attctgtttg cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata
     4681 tcaaaggcgg cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg
     4741 tgtattgcat tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta
     4801 aattagtcct gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac
     4861 atgcgaaaaa atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg
     4921 cagcagcatt atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag
     4981 ataatgatga aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag
     5041 ccgcaggtgt aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata
     5101 tccgagaaga agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct
     5161 ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga
     5221 tgatttcttt gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag
     5281 ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc
     5341 tgattacggc actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga
     5401 ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt
     5461 ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac
     5521 acaacatcat agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca
     5581 atttggctgt ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt
     5641 taattggtgg aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta
     5701 ttggcggaat gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg
     5761 aaattttaga tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg
     5821 gttctgtcac ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta
     5881 aagatatcag cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg
     5941 ccgggaagtc aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag
     6001 ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt
     6061 cctttgtttt gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag
     6121 ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag
     6181 aattcatcga aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact
     6241 tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga
     6301 tcttgatcct agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag
     6361 aagcactcga tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt
     6421 ctgttgtcaa agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa
     6481 cccatgagga attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag
     6541 gaacggagga gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt
     6601 gaagcggtac aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct
     6661 acaagtaaag gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg
     6721 aggcttgatg caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt
     6781 gctgttttat gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt
     6841 agtagggaag tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac
     6901 gatccgtttc tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt
     6961 agataatatt caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt
     7021 acttgtcggg atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat
     7081 tgcttccact ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata
     7141 tgtggatctt cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag
     7201 cggacaacgt gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga
     7261 gcataatcaa agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt
     7321 tccgatgatg caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg
     7381 gttagcatta aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt
     7441 ccagtattca caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat
     7501 ccaattagcg attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt
     7561 caaaccagaa aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt
     7621 agattttggt tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa
     7681 aggagaaatg gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct
     7741 gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg
     7801 agattatgat cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt
     7861 gttctccgga acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga
     7921 agtcatcaat gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg
     7981 ttatgataca gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat
     8041 cagtattgct cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag
     8101 taatgtagac acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg
     8161 aagaaccagc tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt
     8221 tgtcttgcgt gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa
     8281 tggattttat gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac
     8341 ctcgttataa cggggttctt tttatatatt ttatgttgtt tgtttttata aggggcag
//
LOCUS       DACEKQ010000108         8363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEKQ010000108 DACEKQ010000000
VERSION     DACEKQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.113"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..973)
                     /locus_tag="IW671_002424"
     CDS             complement(131..973)
                     /locus_tag="IW671_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0899392.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1010..2476)
                     /locus_tag="IW671_002425"
     CDS             complement(1010..2476)
                     /locus_tag="IW671_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0899393.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2532..4376)
                     /locus_tag="IW671_002426"
     CDS             complement(2532..4376)
                     /locus_tag="IW671_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0899394.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4978..5928
                     /locus_tag="IW671_002427"
     CDS             4978..5928
                     /locus_tag="IW671_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0899395.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5973..6606)
                     /locus_tag="IW671_002428"
                     /pseudo
     CDS             complement(5973..6606)
                     /locus_tag="IW671_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6866..7414)
                     /locus_tag="IW671_002429"
     CDS             complement(6866..7414)
                     /locus_tag="IW671_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899396.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7415..8272)
                     /locus_tag="IW671_002430"
     CDS             complement(7415..8272)
                     /locus_tag="IW671_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0899397.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT         2497 a         1708 c         1309 g         2849 t
ORIGIN      
        1 agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata acaaacagct
       61 ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca tcaatagtat
      121 tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca cctaggtata
      181 caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct ttgatcttag
      241 ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat tcttcctcac
      301 catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca tgtgtaatga
      361 agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac cggaccaaat
      421 tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca ggatcttccg
      481 attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga agatcaattc
      541 catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct ttgggatagt
      601 agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt gcgcgttcaa
      661 ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt tttttatcta
      721 atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca ataggtattt
      781 cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga aatattttat
      841 ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc cctttaccta
      901 atgcaatcat ttctttgcca ttttcttcaa caagtaccac acttgaattc aatacttttt
      961 ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac tactcgaata
     1021 aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc tttttcaagc
     1081 ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca tatcgtttag
     1141 acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta taagctatca
     1201 aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta aagtagtcga
     1261 tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc ataccatttt
     1321 ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa attttcaaac
     1381 cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc ttaaccccta
     1441 aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc atggacatgt
     1501 aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga tcttcatctt
     1561 ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca ggatattctc
     1621 ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag gtcagctcaa
     1681 cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata caaccaactt
     1741 ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca acaaattgat
     1801 gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc tcttggataa
     1861 ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat gtcagccaat
     1921 attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca aaatcatcaa
     1981 ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc atttcataat
     2041 gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag acgttttcat
     2101 aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac aagcgagtcc
     2161 aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct aaatcttctt
     2221 tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca gatttgtctt
     2281 cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa tctttgttgt
     2341 ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg tctacattcc
     2401 aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct tttggaaaac
     2461 ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata ttttttttca
     2521 ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt ggttaccaat
     2581 ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac acagataact
     2641 gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag cttttgtcct
     2701 ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc taactgaaca
     2761 gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa accaatagca
     2821 tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa tatcccttct
     2881 tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt gtcttttact
     2941 tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc tgaaacaacg
     3001 gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata aagaaccaac
     3061 gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca aaccagattc
     3121 attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc cactaaagca
     3181 aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga aaacataacg
     3241 gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc agtaattccg
     3301 aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc ttttgttttg
     3361 atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga agccgggaga
     3421 taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat cattgcttta
     3481 tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt tgcaacccat
     3541 ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac attataacct
     3601 aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat cataggtaca
     3661 aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag ttgagcataa
     3721 aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt atccaatcca
     3781 aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag gatcaatgca
     3841 ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt agtcattgcc
     3901 accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat ctgataggtt
     3961 tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga cttcaatact
     4021 cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa atctaataga
     4081 actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac attgtcacct
     4141 aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat tacttggcat
     4201 tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct catatttacc
     4261 ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa atgggatatg
     4321 ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta attcatggtg
     4381 tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat cctcaacaca
     4441 aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga aacgcataca
     4501 ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg tttaagttat
     4561 attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt aatcattttt
     4621 tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt ttctaagaat
     4681 atcttccaac atatataact cttattctct tctatcttct agcagatgtt ctgtcttttt
     4741 aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga ggttttttat
     4801 ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta ctaaaaaatg
     4861 ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata tcgatattgc
     4921 ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt ttataagatg
     4981 aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat cagtaattta
     5041 gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg aagaacaaat
     5101 gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat caatactcgt
     5161 ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc ggtaaaagat
     5221 gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac agattccatt
     5281 ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca aatgaaaact
     5341 gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac tggagttgga
     5401 aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt tattcatgag
     5461 aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga attattgtcg
     5521 gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga tcgaaatgga
     5581 ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag attgcctcct
     5641 gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag agtcggtgaa
     5701 actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg caaagaggat
     5761 tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa tattgaggaa
     5821 cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga aagcaaaaac
     5881 tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat atagtcagaa
     5941 tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa actttcatct
     6001 acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac cgctaccttc
     6061 acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc tcttaaatta
     6121 gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg tatttggctc
     6181 ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat aattgaccat
     6241 atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac
     6301 taataatact tgatctcgct tattataatc gctttcattt gtcctaagag tcaaatcttt
     6361 ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct
     6421 ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt gagagtcatc
     6481 taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc
     6541 catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt
     6601 tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta cttatttact
     6661 aactcaacaa cagattatca tacacttatt aatgaaatca caatatctca ttaaaacaca
     6721 ccctaaaatc ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa
     6781 aaataaatcc cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg
     6841 tagttactta gttcttcttt gattattatc taactttatt attcattccc caccctttgc
     6901 gcagtgagtc ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat
     6961 catctaattc tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt
     7021 cattaaacaa atcttgttta tcttcttgcg taagattctg tttaatatac tcatctaaag
     7081 cttttctaag caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg
     7141 aagtcgcatt taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa
     7201 actttctgat tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg
     7261 aggaagtttt tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa
     7321 ttgcttgctg ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt
     7381 tcccaaaaat atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct
     7441 cgtgttcttc caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt
     7501 gttttaagag acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta
     7561 agcgcgcatt tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt
     7621 gtttatacat ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag
     7681 caacatataa atttgtatca aattctttca caaaaaagtc catatactca agatattgca
     7741 tcgcatttct catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt
     7801 acatcgcgga tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa
     7861 actcatcaaa taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat
     7921 tatattggtc tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat
     7981 agaggttatt gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt
     8041 cagtcacagt attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga
     8101 agttcatatg cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta
     8161 tcaaggttct ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac
     8221 ttttacctac gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac
     8281 ctcttttctt ttagcaatat tcatttgata ctattataac atatttattt ttagttacta
     8341 ccaaaaaaaa gatatatatg tac
//
LOCUS       DACEKQ010000109         8321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEKQ010000109 DACEKQ010000000
VERSION     DACEKQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.114"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(10..687)
                     /gene="nth"
                     /locus_tag="IW671_002431"
     CDS             complement(10..687)
                     /gene="nth"
                     /locus_tag="IW671_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0899398.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(694..1392)
                     /locus_tag="IW671_002432"
     CDS             complement(694..1392)
                     /locus_tag="IW671_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0899399.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1660..2844
                     /locus_tag="IW671_002433"
     CDS             1660..2844
                     /locus_tag="IW671_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0899400.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2850..3293)
                     /locus_tag="IW671_002434"
     CDS             complement(2850..3293)
                     /locus_tag="IW671_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0899401.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3519..3806)
                     /locus_tag="IW671_002435"
     CDS             complement(3519..3806)
                     /locus_tag="IW671_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899402.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3977..5329)
                     /locus_tag="IW671_002436"
     CDS             complement(3977..5329)
                     /locus_tag="IW671_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0899403.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5602..6048)
                     /locus_tag="IW671_002437"
     CDS             complement(5602..6048)
                     /locus_tag="IW671_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0899404.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6284..7210)
                     /gene="cysK"
                     /locus_tag="IW671_002438"
     CDS             complement(6284..7210)
                     /gene="cysK"
                     /locus_tag="IW671_002438"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0899405.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7350..8237)
                     /locus_tag="IW671_002439"
     CDS             complement(7350..8237)
                     /locus_tag="IW671_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0899406.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2244 a         1719 c         1394 g         2964 t
ORIGIN      
        1 aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt
       61 tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt
      121 gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg
      181 tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct
      241 caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc
      301 atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc
      361 tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat
      421 gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat
      481 tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt
      541 attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg
      601 attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc
      661 ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga
      721 gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg
      781 ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca
      841 cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg
      901 cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg
      961 ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga
     1021 ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc
     1081 acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat
     1141 cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg
     1201 atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc
     1261 aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct
     1321 atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc
     1381 taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag
     1441 caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac
     1501 ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta
     1561 agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt
     1621 tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa
     1681 cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc
     1741 agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc
     1801 tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc
     1861 ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca
     1921 agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt
     1981 atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc
     2041 acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga
     2101 acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata
     2161 tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa
     2221 agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac
     2281 caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct
     2341 gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt
     2401 cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc
     2461 tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga
     2521 atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta
     2581 ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact
     2641 cctgtattca atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg
     2701 ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag
     2761 ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc
     2821 tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat
     2881 cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta
     2941 gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg
     3001 attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg
     3061 atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta
     3121 gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct
     3181 agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata
     3241 atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca
     3301 ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt
     3361 ttgtaggaaa acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc
     3421 cctttttctg attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt
     3481 catcacttaa ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct
     3541 agcacatatg gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct
     3601 gagtagatgc gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct
     3661 ggcatcgtgt aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac
     3721 tcatcaatat tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct
     3781 tgttggacaa aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa
     3841 agctgcttgt tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg
     3901 aagaaagaca actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg
     3961 tcacaaccct ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta
     4021 ggttcaagta attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga
     4081 gatcttctac agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa
     4141 tgtcgtattt ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta
     4201 gctccatcat tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata
     4261 cagtcgcttg gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac
     4321 cgatcgtcca gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt
     4381 tatgatctgt caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag
     4441 gaatatagtt ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa
     4501 aaatgtctgg attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg
     4561 tatctacttt tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca
     4621 tatctgtatc aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg
     4681 tgataaattc agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt
     4741 tttctaagat atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat
     4801 caatcaatgt cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat
     4861 atccaccacc aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg
     4921 cttgattgta gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga
     4981 taataggcca tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc
     5041 ctgttttcag atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt
     5101 gttccatttt aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc
     5161 ctgctgggtc ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa
     5221 tattgtcatt tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt
     5281 ttacagctgc agtacctgca tgagtacagc caattactac tactttcatt tatggtccct
     5341 ccgattaaca ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag
     5401 tttaatgctt gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga
     5461 acccaatatc tacaagattc ttattttaat aatattcact tttatatcta agattgtgaa
     5521 taaaagacaa aaaaataaaa aagctagcgt tttttttaca cagctagctt tttttaggta
     5581 taacacttgt agaaactgtt tttacatttg cgcctttttt gctgactctt tacaatccgg
     5641 acagagtcca tacaattcca accgatgttc attaatctca aaatcagtca gtctgcttgc
     5701 agccatttct atatcttcaa gccctgggta atgaaaatca acaatcttac cgcagttttc
     5761 acaaattaca tgataatgac gctttgaact gaaatcaaat cggctagaag catcgccgta
     5821 cttcatttct tgaacaaatc caatcgaagt aaataagcga aggttattgt aaacagtcgc
     5881 aacactcata tttggaaacc gatgttcaag tgctttataa atctcatcag ccgttggatg
     5941 ggaatgatgt tcaatcaaat attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt
     6001 ctcttttttt aacttttcga taacctcttc gactaactta ttttccatgt aaacacccca
     6061 cgttgttatt ctcgtcttat catactattt ttacatttta aaatccactt aacatgaaaa
     6121 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
     6181 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
     6241 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
     6301 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
     6361 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
     6421 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
     6481 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
     6541 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
     6601 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
     6661 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
     6721 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
     6781 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
     6841 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
     6901 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
     6961 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
     7021 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
     7081 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     7141 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     7201 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     7261 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     7321 atggtaattt ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa
     7381 gaataactgg acatagacat gccatttttc gccattagat catcaacaga catgccaaat
     7441 ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt
     7501 gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt
     7561 gtacttgtgg ttgttgtagc cgaacctgtt gatggcgtat caaaacgggt taaattataa
     7621 gtattaatca atgagttcaa tttggcccca taatttggat cggtagcata acgacctgtc
     7681 aagtacgctg ttgcatcata aaaacttgtt gtattgcttt tccacacgcc agcataatga
     7741 taatcgcccg tggaaaaact ggttgaacga agaacattcg catggtcttg gaaggattcc
     7801 caataagaag aatattttcg gaatggctca ttcatcgtta cccattgtcc gtttacatat
     7861 tctttggttg ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta
     7921 taatttggcg cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg
     7981 atcatgactg aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt
     8041 tgatcgataa acgcttgagg atcagcgact gtttgagatc cttgttcatc tgcatgaaca
     8101 tgttgcggta ccgctgtaac catcatcggc ccaactaaaa gactcgttcc aattaaagga
     8161 gcaatcgctt ttttattgat caccattttt cttttaggtc tatggtgccg ggcactgcgg
     8221 gtcttcagct cttgcattca aattcctcct caaaataaaa aaacttaata aaaagttctc
     8281 ttcttaaaac aaacttaatt aattaaatta tacttttaat t
//
LOCUS       DACEKQ010000110         8138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEKQ010000110 DACEKQ010000000
VERSION     DACEKQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.115"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            171..1910
                     /gene="yfcC"
                     /locus_tag="IW671_002440"
     CDS             171..1910
                     /gene="yfcC"
                     /locus_tag="IW671_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0899407.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2067..3251
                     /gene="iadA"
                     /locus_tag="IW671_002441"
     CDS             2067..3251
                     /gene="iadA"
                     /locus_tag="IW671_002441"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0899408.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3362..3565
                     /locus_tag="IW671_002442"
     CDS             3362..3565
                     /locus_tag="IW671_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0899409.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3666..4328)
                     /locus_tag="IW671_002443"
     CDS             complement(3666..4328)
                     /locus_tag="IW671_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899410.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4341..5843)
                     /locus_tag="IW671_002444"
     CDS             complement(4341..5843)
                     /locus_tag="IW671_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899411.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNLKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6391..8070
                     /locus_tag="IW671_002445"
     CDS             6391..8070
                     /locus_tag="IW671_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0899412.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
BASE COUNT         2540 a         1439 c         1752 g         2407 t
ORIGIN      
        1 taaatggaaa gagaaaaaaa taatcataca taggagaaaa aaaacaaaat agaaaaaagt
       61 gattttctaa taaattattc atgtggaaat gtcattttta ctgtataatt catttgatta
      121 tccatttttt attcatcaaa attactgaaa aaagaaagga agaaataaat ttgaagaaaa
      181 caaattcttc aggaaaaccg aaacgcgact tcactaaatt atcgacaccc catacgtatg
      241 tcattatttt tggtgtagtc atctttgcat ggatactgac ttttgtcgtt ccagcaggaa
      301 agttcagcac gcaagatatt gaatacaaag atgcaaatgg cgaaacaagt actcgtactg
      361 ttttacgtca agattccttc cgttatgctt acgagctgga taagtcgtat gtgtttgatc
      421 aattagaaga actgcaagat catccagcag aacgtgaaaa actggatgtt ccggaaaaag
      481 gattggaaaa agtcattgct gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa
      541 tctccttgac agatgatgtg ctttacgatc agtacggcga aaatatctat gatacttcta
      601 aaaaattgca taaaacagcc aaaatatggg gaacagatga ttttggcggc tttggtttct
      661 taaattttgt ttttgaagga ctagtatctg gagataagta tggatctgct gtagggatcg
      721 ctgcattgat ccttgttgtt ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg
      781 acgcaggtat ctatgcgttt atcagtaaaa ctaagggact ggagcgttta gctttaccgc
      841 tattattctt tgctttttca ttcggtggcg ctacattcgg gatggcagaa gaggtaattc
      901 cattctcaat ggtgatggtt ccatttgtca ttgcgttagg atatgactcg atcgtagcag
      961 taaccgtgac ttatgtcgca tctcaagtcg gaaatgcgac ctcttggatg agcccgttta
     1021 gcgtagcggt ggctcaaggg attgctggaa tccccgtgtt gtctggtgcg acattccgat
     1081 tgattatgtg ggtagttgtt actgcattag ccgctggtta tatgatgata tatgcagaaa
     1141 aaatccgtaa aaaaccagaa agttcgttga cttacaaatc agatgattat ttccgttctc
     1201 atatcaaaaa gacatctgat gaaaataaaa aattcatgct tgggcataaa ttgatcttat
     1261 tagagatgtt agttgtcctt gtctggatcg tttggggcgt gactcaaaaa ggctattaca
     1321 ttccagaaat cgcttctcaa ttctttgtga tgggattagt tgctggaatc attgctgttc
     1381 ttttcaaatt agatggaatg aaagtcaatg acatcgcttc ttctttccaa tctggtgcag
     1441 ctgatttagc tggtacagca atcgtagtag gtatggccaa agggattctt cttgtcttgg
     1501 gaggatctga tgcatctgtt ccttcagctt tgaatacgat tttgcatggg atcgggacgg
     1561 cgttgaccgg tgttccagct gtcattggtg cttgggcgat gtacatcttc caaagtttgt
     1621 tcaacttggt tgttacatca aattcaggac aagcagcttt gactatgcca atcatggctc
     1681 cactggcaga tttagtaggt gtatccagac aagtagcagt tcttgcttat cagcttggtt
     1741 caggttttat ggacgctttt acacccgtat cagccagctt gattggtgtg ctaggtgttg
     1801 cacggatcga atgggcaaaa tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg
     1861 ttttaggaac tattttcatc atgattgcca ttgcaatagg tctgcagtag tactggagac
     1921 ctgtaaataa aaaagtaatc caaacagtag ataaggactc caagaaccgt cattttacgg
     1981 taaaatcaaa ggagtaaaga tcctatcgtt tggattattt tttaagatcc tatcatcagt
     2041 tatacttaaa gaaagaggaa aaatgtatga aaatcattcg acaaattgaa gtgtttgcac
     2101 cagattactt aggaaaaatg gatgtactga ttgccggtgg gaagattttg gctgtagaag
     2161 aacagctaga aggcggctac gaaggagtgg aagtagaaga actttcaggt gagggaaaag
     2221 tactaacccc gggtttcatt gactgtcatt tccatatttt aggtggagga ggagaaggcg
     2281 gttatcaaaa tcgaacgcca gaagtcacat tgagccaatt gactacagct ggtgtaacga
     2341 cagttgtcgg ttgtttggga acagacggag aaggtagaga tatgactgcg ttgatcagca
     2401 aagcgaaagg tctggaagca gaaggaatct ccacttatgt atacgaaggt tcgtaccgat
     2461 tgccggtaaa aacagtaaca gattctatca tcaaagactt tttgacaatt gataaaatta
     2521 tcggaatcgg agaaatcgca gtatccgatc accgttcttc tcagccaagt tttgaagagt
     2581 tcgctcgtgc agtagcagat gctcgtgtag gaggcatgtt atccggaaaa gctgggatca
     2641 tcaatgtcca cttaggcgga ggaaaaagaa agctagagct attgacacga atcgtagaag
     2701 aaacagaaat tccgatcaca caatttttgc ctactcatgc aaatcgcaca ccagaattat
     2761 ttgaagcttg cgtggcattt gccaaacgtg gtggcacgat tgacttcact gctagcgaag
     2821 atcctgattt ttgggagaag acagatggtg aagttcgctt cagcaaagca ctgaaacgat
     2881 tgatcgagga agatgtttct ttagataatt tcacgatgac ctcagatgga caaggaagct
     2941 tgccttattt tgatgaaaac aatcatttcc tagggttagg agtaggaagt gccaaggcat
     3001 tgctagtagg gatcaaagaa gctgtgcaaa aagaaagtat ccctttggaa atcgccttac
     3061 gagctataac cagtaatcca gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa
     3121 ttggtgctga tgcagatctg tgtatcttgg ataaagaaac gcttgatatt gatacagtta
     3181 tcgctaaagg agaaataatg gtccaagaaa aagaagtcaa agtatggggg actttcgaaa
     3241 aaagttttta atcaacagct ctaactatct tttttagaaa acgagcgttc tgtttgtctg
     3301 tatctgttgt tgatgttacc ctgaaggtag atcaataaac aaactaagag gaggaagcac
     3361 aatggcagat ttaaaaggac gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa
     3421 agaagcatac ggcaaagcaa cagacgacaa gagcaaagaa acagaaggga aagcacaatc
     3481 actaggtgct gacatcaaag aaaaagctcg aaatttaggc gacgaagtca aagacaagtt
     3541 ttctcgaaaa gacgaaaaaa aataaaaacc acttattact aattgaatca agcaaataaa
     3601 aaatccgaac catatctgcg agacagatat ggttcggatt ttttatttga tcattatcct
     3661 gttttttaaa cttcgatttt ttgaaatttt ttatttttta gttcaaagac ttcatcacag
     3721 ctttttgcta cttcaatctc atgagtcacg atgataatac agcgattttg ttcatgggcg
     3781 atttcttgga aaagtttgac gatatcttgt gaagttgttt catctagatt tccagtaggc
     3841 tcatccgcta cgattagttg atgttcacag cagatagcac gaacgatagc gactcgctgc
     3901 tgctgacctc ccgaaagttt ggtcacctgt ttttttgcta atatttcatc aattcctaca
     3961 cgagccagtc gatctaaagc aacttgtcga taattctctt gcttggtctt agtgatttcc
     4021 attgctgcta tgacgttgtc tagagccgat aaataaggaa gcaagttgta agcttggaaa
     4081 ataatcgaga catcattttt acgataactt cttaacccga ttttgttcag tgaacgatct
     4141 tgatagtata cctttccttc ttttggtgca tctagtccag aaataagcga aagaaaagtc
     4201 gtttttccag aaccacttgc tcctaaaata gcgtacattt ttccagcttc gaaattcagt
     4261 tctacatctt tataaagcgc atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc
     4321 cgtaacatca aattcctcct ttagtttaat aggatttttc ttgggtttaa tcttaatatc
     4381 cctatagaag ctaaaaagac agaaaacaga ctgattccaa atgctactcc ggctaatagt
     4441 cccaattgtg caggtttgac attgatttcc aattcgctga cttgatcaga aacagcaaat
     4501 ggattattcg tggttccttg ttgaccatcg cgttgaccgc cttgttcttg tctgttacct
     4561 ggcatttgtc caccgccagg agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca
     4621 agtagctgct gtcccacggc attcgcgacg acgtttccgc taagcgaagc aactcctaat
     4681 gcaagaatca tacaaatagc cacttcggta aacaattgga gaatgatttt acttctggat
     4741 tctcctaaag atagcaagac gcctaactca tgccgacgct cccgaatggt aatcataatg
     4801 atcagagtaa gtatgataat accggctaca gccactagta aaacaacatt tttagaaaaa
     4861 cttgccacgt tgtttaaagg ttccaacatt gactgataca tggaatcatt tgattgtaag
     4921 ctaaatgtat ctgtgtcgat cagcttctca gcttctttga gaaactcatc cattttatct
     4981 ggatcagata gagtgtaaat agcagaatct atcgtgttat cgtcagatgt cccatttagt
     5041 tcatttgcaa aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa gttgaatttc
     5101 atacccatac tgccgcttgt ttcgctggat tcataaattc ctttgatcgt catttcataa
     5161 gtggtatctt catcttcagt actcgtgatt gtgaaagtat ctccgactgc caggtcattt
     5221 gtttctgcta gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt
     5281 ccttcaccat ccgtgatttt tgctgttcca tcagaaaaat tactattttg tgcagttgct
     5341 gacacaccac tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc cattcctcca
     5401 gcaggttcac cgcctgcatc agtagtttct gaactgtctt cacttgaatc agaactagtg
     5461 atggcagtta ttccatcttt tgccatagct gaggcggaac tttcaaagga ataactcttt
     5521 aaattatcta attcagctat tttttccgcg tccgttaagg aaactggtgt aagagaaaaa
     5581 ctgcctggat ctggacgttc ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt
     5641 ttaaatgaag cttctcgatt ggcacttaaa gtcactgttg ctccaacttc tttttgtgct
     5701 tgattcgctg cttcttctgc agcgcttcga atcgtgaggc cagctagcac aaaaataaga
     5761 atggctgtga acacggcaaa caataacaat gaacgtcccc attttacttt tgtacttttc
     5821 catgcacgtt tcataaaatt catattttgc atccctccta gtagatgcat ttgataatag
     5881 acaacctatc ttaaagctag cttaaatctt atgctgtttg cacaccatca acatgaggtt
     5941 ctgtatatat aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat
     6001 gtttcaaacc gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct agatgctaca
     6061 gagggatttt tacgtgcata gttgttctaa caattgctga tactagcgat ttagtttatt
     6121 tgttgtataa taatggggaa cagctgaata cttatataag tattctggct atagcgtttc
     6181 gcaaaagatc cattttgtta atgaagtata tccaaaataa aatgcccatc caactgtcag
     6241 aacgcggatg tgctggtaat tttcctgctg attctccata agttgagaaa tagtgatttt
     6301 gcatatgctt cattggaatg gctcatatat ttagataaag aagaaaatcg acatctttaa
     6361 acttggatgt cataagaaga ggtgaagttt gtgagtacca ttaaaattat cccattaggc
     6421 ggcgttcgtg aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga tatttttatt
     6481 ttagattgtg ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct
     6541 gattttacgt atttagaaga aaacattgat cgtgtagctg gtgtcttttt aacacatgga
     6601 catgcagatg cgatcggggc acttccttac ttgttgtcaa aaatttctgt accagtattc
     6661 gggacagagt tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa
     6721 ggatttaagg atttccatgt ggtagatgag catacagaaa tagactttgg tgaaacagta
     6781 gtgagctttt tccgtaccac tcatacgata ccagattcag tggggatcaa tctgaaaaca
     6841 aaagaaggta gtatcgttta tacgggagat tttaaatttg accaaacggc tatcccaatg
     6901 tatcaaacag attatgcacg attggcggaa atcggcaaag aaggagtctt agccttacta
     6961 agtgaatcgg caaatgcaga aaatcctcag cctgtagctt ctgaagccca aatagcagat
     7021 gaagtatttg atacgatcaa atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt
     7081 aatttgcaac gagtgcaaca agtattggat gcagcagaaa aagcacaacg aaaagttgtc
     7141 ttgactggtc aagatttcga acgaattatt cgcacagcga tgcgtttaga aaaacttcaa
     7201 ttaccaagtg aagatctgct tgttacgttg aaagacatga aaaactatga tccagaagaa
     7261 ctgatcattt tagaaactgg ccgaatgggt gaacccatca agtctctgca aaaaatggca
     7321 aacggtacac accgtaatgt caagattgaa gaaggagatt tggtttatat cactactacg
     7381 ccaagcatcg cgatggaaac catcgtggcg aaaacggaag atatcattta tcgagctggt
     7441 ggaacagtga agttgatctc tgagaatatg cgcgtttctg gccatgccaa tcctaatgac
     7501 ttgcaattaa tgatcaattt gatgaaacca acatattttg tgccagttca aggagagtat
     7561 cgtgaattag ctgctcatgc agatttggct catgcagtag ggatgccata caaaaatatc
     7621 tatattactg gacgcggaga cgtgctggaa tataagaaca agcgtatgag tgttgcagga
     7681 actacaagtg cagaaaatgt aatgattgat ggattaggca taggtgatat cggaaacatt
     7741 gttttacgtg atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt tgtcactatc
     7801 agtcggcgag aaaagaaaat catttcaaaa cctcaaatta cttcacgggg attcgtttac
     7861 gtaaaagcta gccgtgatct gatgaaagaa agcagcaata tgattgaaac aatcgttgag
     7921 aaacatttag aaagcaatga ttttgagtgg agcaaattaa agcaagacat ccgagatcaa
     7981 ttaggccgtt acttatttga acaaacaaaa cgccgcccag tgatcttgcc agtcattatg
     8041 gaagctactc aacgtcgaaa taaaaaataa acaaaaagag taagatgaac ctctctatag
     8101 agtgctcgtc ttgctctttt tttgcgaaaa acaaataa
//
LOCUS       DACEKQ010000111         8099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEKQ010000111 DACEKQ010000000
VERSION     DACEKQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.116"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(264..443)
                     /locus_tag="IW671_002446"
     CDS             complement(264..443)
                     /locus_tag="IW671_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899413.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(440..790)
                     /locus_tag="IW671_002447"
     CDS             complement(440..790)
                     /locus_tag="IW671_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899414.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(783..2108)
                     /locus_tag="IW671_002448"
     CDS             complement(783..2108)
                     /locus_tag="IW671_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0899415.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(2277..2861)
                     /locus_tag="IW671_002449"
     CDS             complement(2277..2861)
                     /locus_tag="IW671_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899416.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(3072..3275)
                     /locus_tag="IW671_002450"
     CDS             complement(3072..3275)
                     /locus_tag="IW671_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899417.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(3285..3707)
                     /locus_tag="IW671_002451"
     CDS             complement(3285..3707)
                     /locus_tag="IW671_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899418.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4021..4635
                     /locus_tag="IW671_002452"
     CDS             4021..4635
                     /locus_tag="IW671_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899419.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(4800..5804)
                     /locus_tag="IW671_002453"
     CDS             complement(4800..5804)
                     /locus_tag="IW671_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899420.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(6263..6520)
                     /locus_tag="IW671_002454"
     CDS             complement(6263..6520)
                     /locus_tag="IW671_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0899421.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(6513..6782)
                     /locus_tag="IW671_002455"
     CDS             complement(6513..6782)
                     /locus_tag="IW671_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0899422.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(6913..7092)
                     /locus_tag="IW671_002456"
     CDS             complement(6913..7092)
                     /locus_tag="IW671_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899423.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(7473..8039)
                     /locus_tag="IW671_002457"
                     /pseudo
     CDS             complement(7473..8039)
                     /locus_tag="IW671_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2503 a         1537 c         1178 g         2881 t
ORIGIN      
        1 gttatccagc ttgcaaccgt tccttgtggg taggcatata actcacaaaa ttgttctaca
       61 gtgattccta aattcttgat aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg
      121 atagacatgg tgcgtaagcc tactttcttt tattattgcg tttgcaataa tagtttaaca
      181 cacgcattat tctagtatct actcctattt gtaaaaacaa aaaaggattc atagagataa
      241 ctctatagaa tccgtaaaat accttatata tataacggga agatataaaa cctaaaatta
      301 tgataacctg ctgcagcttc tacatagaaa gagaagcgct caacaccaac ctcatttatc
      361 aaacgttggc cataacccca aatctcttct gatagctgat caaaggttat atactttagc
      421 tcatattgct ggataatgtt cattctaatc gactccattt attatcctgg taaaattcga
      481 tatgacgtat ttcgtcgtag tgaattttgt cttttccaac gtagatcccc agctcatcac
      541 tacctaagat cttgccaatg atatcttctg gatagtatcc ttcagcgttt cgttcttctt
      601 tttgaataga tactgatcta ttttttatga ctgcagcgtt caatacttcc gcaatctctt
      661 cagtcgtcat ttgttctttt gctaaatttt gactggttcg ttgttcattg tcggcatcaa
      721 tttcttcagt atgatcagat aaataaaacc ctgcccactt taaacgtcca cgatcattgt
      781 aatcaatcat ttttaattcc cccatttccg cccgcatgac caccaacaag tgtgcttcta
      841 gtaatgcttc tagcgccttc taacattgaa gaagcatgta caatcgacgt aaaaccatat
      901 ttttgccgaa tcttgtcaat aattttatct aaattttctt cgtcaatttg cttttgtgga
      961 tcactaaata aatccagctg aagcgaatcg gtgtacacaa gcttgctata ggttattcca
     1021 acatgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca ataattctgc
     1081 aattcctttg tattatctgt gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt
     1141 tctaagatcg acgtgccaat gtgtaaatgg acacaacctg ttttacagtt atgctgccta
     1201 atgcgtatcg ctacttgttc cgccatttct tttaccacca gctcgatttc atttcttcta
     1261 gtataatctc gtggtaacac ttgtgaattt ccatatgatt tttcctttgt cggttctggt
     1321 gggattgcta tatctgttcg atcgatccca ttagcatgga agtacagctg taatccaatc
     1381 acacctaatc tattcttaat cgtataagga ttccagttag caaggtctct aatgctcaaa
     1441 attcccattt ggttgagcct ttttttcatt cgcgaaccaa ttccccaaaa gtccgtcatt
     1501 tcgggaatat tccatacttt ctcaggaacg ttttcgtacg tccattccgc aataaatcct
     1561 tcattatgct ttgcttcatt gtctaaggct aatttagcca aaaggggatt atcgccgacg
     1621 cctaccgtag caatcaaacc taactcttct ttaatacgtt cttggatcat ttgagcgagc
     1681 ttcttccttc gctgacttcg tgtttcttca gtcgtaaaaa gattcagtga ttttgtcact
     1741 tttaaaatcg attcatcgat cgagtaaatc agtagatctt catcagccac atatcttctg
     1801 aaaatattat ttacctgcat atttcgcttg atatataggt tcatacgtgg tggaacaacg
     1861 tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc tactcacatt tgtaatacca
     1921 taccgctttt ttgcttcagg agaagaagct aatatcaatc ctgaaccagt attatcagat
     1981 cgactcataa caacaagttc tgttgttaat ggatctaaat ttctttctat acattcgaca
     2041 cttgcgtaaa agcttttcat atcgattaaa aagtaatcat ttactggctc ttttgaataa
     2101 tccatcataa ataacactcc attcttccac attatacaaa catatgttcg atgttgtaaa
     2161 gaagaaatag ctaaatattt gagaataagt tatcagtaga cacttacaaa aaattctcat
     2221 actattgtca cttttctaga taaatggcaa aaattttcag cattaaatga caaaaactaa
     2281 tgagaatttg tctttccatt tttctcctcc atttttgcca aataacgata aatggtggtt
     2341 cttgagacgt tccaccgttc cgcaatagtc ttaataggtg taccgctatc cactaaagtc
     2401 ttcagtaatt ccaaatcttg ttcagacaat ttttcaggac gaccaccaaa ccgtcctctt
     2461 gcacgagcag ctgctcgtcc tgctgcggat cgttccagga tcaaattacg ctcaaattct
     2521 gcaaatgccg caaacaaatg aaacatgagc tgtcctgtag aacttgattt gtccatcgta
     2581 atattttctt gaatactatg aaaactcaca ccacgctcat tcagtcgatt gacgattgaa
     2641 atcaaatctt ccatatttct acctaaacga tctaagcgcc aaacgacaag tgtatcgcca
     2701 gagcgaacaa aatcaatggc catttctaat cctggtcgat tactttttgc cccactcata
     2761 tgatcagtaa aaattttttc acaaccaaat ttatttaggc tgtcttcttg taaatccaaa
     2821 ttttgtaaac cagtagatac ccgggcgtat cctattttca aaaaaatcac tccttcatta
     2881 attttattgt aacataactt tatccatata gtttaactga acatagatat tgttatgggt
     2941 ttatgaacat gaaatgaatt gtttttttga aaaataaagc aaaaaaagaa agtgtccaga
     3001 aatgggtgat tttatgaaca ctttcaatta gaaaaaaaga atgtcgacta gacactcttt
     3061 ttttaaaatc attatttttc tttttgatat gttttttggt ctcttttttt tcttttttct
     3121 tcagaatctt ctagattaga aattgatttt tgaatttttt gtgtctcctg taaagttcgc
     3181 tgaacttgta aagctgattc ttctttcggt aaattaattt tacttagaat atcgctatct
     3241 tgataaaaat ttggtgtgtt cacaaattct ggcataaaaa atccctactt tctagttaag
     3301 gttcctgaat aagtcgcaat ccaattgttt ccagctgatt tataactttg taaatacaat
     3361 tttccatagt accaataacc atttcttttt tctttccaat tccgtgattg aggaggtcta
     3421 gaaggccaac caactttatc aaaaatcatc gttcctgtca ctcgtatcgt tttagaagct
     3481 gctcgagcaa gtaatggaga aactattaaa taattttcag taacagttaa gtcgttagtt
     3541 gggttaccaa tggctattcc gttcctatct aaaggagtta attcttggtt attatctact
     3601 acagtagttg gttcatttgt actatcagca aatgtaggaa ttgtagttcc agttacacta
     3661 attccaatag ttaaaatacc taaagataaa ataaacttat ttttcattgt ctatcacctc
     3721 gaaatcaaaa ataagaaaac gttttcttaa taaatcttaa cagataaatc aagttatata
     3781 ctagaaaatt cataaaatat atttttaatg tcataatcgt ttaaattttc ttgattgaca
     3841 tttatttaca aaaaatattg ccatttaaaa agaaaagtga cacatactat ttcctccatc
     3901 attagttttt gtctatataa ggtttaccct aaatagacac tggtataaca ttgaaaaata
     3961 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     4021 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     4081 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     4141 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     4201 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     4261 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     4321 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     4381 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     4441 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     4501 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     4561 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     4621 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     4681 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     4741 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     4801 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     4861 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     4921 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     4981 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     5041 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     5101 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     5161 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     5221 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     5281 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     5341 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     5401 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     5461 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     5521 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     5581 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     5641 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
     5701 atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     5761 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     5821 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     5881 aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa
     5941 atttaaaata gattattaaa cctattgctt attctattca gtcatatata taagcgtcat
     6001 cctttagtaa ctaacatccc acactacaat agtattataa catatctatt caacttcaaa
     6061 ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa
     6121 ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat
     6181 attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga
     6241 ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt
     6301 cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag
     6361 ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat
     6421 cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa
     6481 gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag
     6541 tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg
     6601 acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat
     6661 aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca
     6721 ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc
     6781 attcctttca ccccttattt atttctataa aacaattgta cattatattg tacaattaag
     6841 caaatgatta atattctaac ttcttataat taaaagtgct atcactttta gtttttagga
     6901 taaaaagtga cgtcactttt tgagtgctgg tacctcaaat cctgcttttt cttgggcaat
     6961 caataaatct aacgcaatac acataatcct ggattcactt ttgccgtact tctgctttag
     7021 ttgctgcaat tctttttgtc tcttttcatc caacgtaacg ggtaaccgtt tatttcctat
     7081 tgcaattggc attagtactc tctcctttca tcgaaataac cctatgaaaa atatggctgc
     7141 aatcatcttg aatagtagac tcacaagggc taatacaaag cgccaaaaga tcaaacaaaa
     7201 taccagtatt gccaaaaata ctacgaagtt taaatgcaca gctgcagcat ggcgcataag
     7261 tatttcgcca acaaatagga ctaacaatag aggaatgatt ttattcatat ggatctcctt
     7321 ctttctctta ggttatcggg tgttgcccgc ttatttcgca ccacctaagc ggcgggggcg
     7381 gttggagcga ttggtgcgaa ataagaacca ttcccttagt tactaggttt tttgtcctta
     7441 tttcacacat tctcactttt cggtataaat gattattgcc tttttttcct ttcaaatcgt
     7501 tgatatggca ttcgtttacg gtcaagttca ttttggattt cttctgtatc gtcgtaagta
     7561 taggtgtcta aaaaatcagt taaagtcgtg taccagtcgt cgcctgggtt atctacttct
     7621 tctacatatt ccctcaaaaa tgcttcgggt acagcgagtt cataatccat tcccattaac
     7681 acgatagatt ccgttccaca cgtcaaaaat ctagtaaata caaggtaata atcgtctatc
     7741 gtcgcatctt tgctgtttag ctcttccatt tcttcttcca ttaattccat gctctcaaaa
     7801 attgaataat attctgattc tgagtaatag tgtttcctgc aaatcgtgta gctgacaaag
     7861 ttttcgacca gctcactcct ttctaggtaa acattcatga tgtatttttc tgtttctagt
     7921 cgtagaagct ccgttccttt ttttaccgtt tcaggacgac gtttatacac gtctaaacga
     7981 ttatggctga ctgtgacctt cgtttgactc gtttcacttt ccaaatacaa ttgttcttcg
     8041 ggttctgttg caaagtttta aatctactat caaataaggt agaataatag aaaaagata
//
LOCUS       DACEKQ010000112         8058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEKQ010000112 DACEKQ010000000
VERSION     DACEKQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.117"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW671_002458"
     CDS             complement(9..530)
                     /locus_tag="IW671_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0899424.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW671_002459"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW671_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0899425.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW671_002460"
     CDS             4610..5497
                     /locus_tag="IW671_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0899426.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW671_002461"
     CDS             5494..6663
                     /locus_tag="IW671_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899427.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW671_002462"
     CDS             6687..8042
                     /locus_tag="IW671_002462"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0899428.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2589 a         1486 c         1827 g         2156 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaagaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaa
//
LOCUS       DACEKQ010000113         7853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEKQ010000113 DACEKQ010000000
VERSION     DACEKQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.119"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            230..847
                     /locus_tag="IW671_002463"
     CDS             230..847
                     /locus_tag="IW671_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0899429.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            1075..1413
                     /locus_tag="IW671_002464"
     CDS             1075..1413
                     /locus_tag="IW671_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899430.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            1398..2252
                     /locus_tag="IW671_002465"
     CDS             1398..2252
                     /locus_tag="IW671_002465"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0899431.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            2399..2632
                     /locus_tag="IW671_002466"
     CDS             2399..2632
                     /locus_tag="IW671_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ0899432.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            2830..4734
                     /locus_tag="IW671_002467"
     CDS             2830..4734
                     /locus_tag="IW671_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0899433.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5088..5453
                     /locus_tag="IW671_002468"
     CDS             5088..5453
                     /locus_tag="IW671_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899434.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            5470..6081
                     /locus_tag="IW671_002469"
     CDS             5470..6081
                     /locus_tag="IW671_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ0899435.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            6109..7770
                     /locus_tag="IW671_002470"
     CDS             6109..7770
                     /locus_tag="IW671_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0899436.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
BASE COUNT         2483 a         1280 c         1755 g         2335 t
ORIGIN      
        1 taaaagtaga agctgattta gggattggaa ttctttccag tcctttttcc attttcttga
       61 tgtgcatcaa tttttttgta gtaagagact tatataataa aagcagcgaa tataaacaaa
      121 ataaataaag ggggagaatg aattgactaa acatacccat catgaaattg gcgaccacgc
      181 agcctgtatc cgcttggttc caatattcaa tcacttagag gatagcgcga tgaattacat
      241 tgctgaaaaa gcccacacga aacagtatca aaaaggagag tttctgtttc gttcacaaga
      301 aaaagaggat actttatata ttatcaatcg tgggaaaatc cgtatttatc gattggcaga
      361 ctctggcaaa gagcagctag tgcggatctt gaaccctggt gatttcaccg gagaatggac
      421 cttgttcaac ccggatgcag tacacgagga atatgccgaa gcaacccgag ataccgttgt
      481 ctgtatgatt cagcaaaaag atgtccaaga ttttctggaa caatacccag ctatttcttt
      541 aaaactattg gcggaaatgt ctaaacggtt agaaagttca gaacgtcaaa cgacacaagt
      601 agccgtggag agtgtcatta cccgtttagt tttgtttttg gcagaaaatg tggaacctga
      661 aatgggcaac tctcccacca tcaccctgcc gatggcaaaa aaggacattg cttcctattt
      721 aggaacgaca cctgagacca tcagtcgcaa atttggagaa ttggaggacg aaggattgat
      781 tcaacagctg tctgggaaaa ggatcaagat tcaggattta gatgatttat tgctttacag
      841 cgaataatcg gacacacttt tttctacacg ttggtgttct gtcgctcttc attaacaacg
      901 ggacagaact cagtgctttt gcttccatct gtccgttttt ggctttttta agacagaacg
      961 accttcattc attggaaaaa taaaagcaca aacatgataa ccctcaagtt tttttgtttt
     1021 atttttcttt aagatagggt tgtcgaacaa aacgtaaaaa taagtagggt gaatgtgcat
     1081 tgtttttggg tgatagttgc catcacgttg tcagcagcgg gggcattctt tgtcgtccct
     1141 ttcaccaaga acacgattgg cggcgaagca aaaacgtcta acgttgttga ggcagatagg
     1201 ttagctatgg aaaaagaaga ggaagttgta aaaggagccg gcgtaatcgg acacggaaaa
     1261 gtggcgtatt acgaagcgag tgaccaggga gataaaagtg tattcactgt ttggtttcca
     1321 tggagcttag ccggaatctt gttcagcatc accatgacta gaatgacgcg aaaaattgaa
     1381 gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat gtggtggaga
     1441 ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata ttagcggctc
     1501 tccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc attatcgata
     1561 tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat gtgttatttg
     1621 aacttggtta ttatctgcaa aatccaggac aaatccttgc gatttggaat ggcggcattg
     1681 cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca aagaaaaaag
     1741 gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta gcccagtcca
     1801 ttggtcgttg gggcaatttt atgaatcagg aagctcatgg tgggccggtt actcgtcaat
     1861 tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg aatggtacct
     1921 actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt gtcctcatcg
     1981 tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg agttacgttt
     2041 tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt ttatggattg
     2101 gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc gcgattgtag
     2161 tatggtttat acgcagacaa gattatccac caatttctta ttattctgat ggcaataagg
     2221 ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa gtgcaaaact
     2281 tgatatgcgt caattttcgt ttgctaaaag tgagctatac tacagttgta aagaggaaaa
     2341 ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga taggaattat
     2401 ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc aaaaaattga
     2461 aggtgccgtg aaatcagtta acggtattga taaagacagc attaaagtat tattcaactc
     2521 cagcaaagtc aaaaccaatt ttgattcagc tgtcacttcg attgaagaga ttcaaaaagc
     2581 tatcgaaaac gtaggctatc cggttcttaa agcaaaagtc aaggccgttt aacagattaa
     2641 atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt ttgttgcaat
     2701 ccgattcgag gtgcttagaa acgataaaag agaactttct aaacttgatg tcagtcaatt
     2761 aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt aaatttggag
     2821 ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt catcagtgga
     2881 ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg actttcagag
     2941 tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca agcgtttcaa
     3001 gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc gattggtgcg
     3061 ctttttattc aaaattatga agaatcggct attgtcacgt tcttattctt attcggacac
     3121 tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt gactgaaatg
     3181 gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt agaagtggat
     3241 gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc agtagatggc
     3301 atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga agctattccg
     3361 gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa cggaacgatt
     3421 caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat tgaactcgtg
     3481 gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt ttctaaatac
     3541 tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca aaatattgag
     3601 ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg agtgcctgtc
     3661 tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa agggagtgaa
     3721 gtcattcaag acttcagtaa tgtggataca attgtatttg ataagacagg tactttaact
     3781 gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc tgaaacgctt
     3841 ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc ggtcttaaat
     3901 caaattggag aaacaaactt ttatcctgtt gaaggaactg aagttgtgaa aggcggcgga
     3961 atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt gatggaaaaa
     4021 gaaaatgtca ctctcagcaa aaaagtgcaa aaagatgtca aacggtttga acaacaaggg
     4081 aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg cattcgcgat
     4141 caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg cgtgaaaaat
     4201 ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg cgaacttggt
     4261 ttgaccgaag ctcacggcta catgttgccg gaagacaaat cggcttatat cggaaaactt
     4321 caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag tccgtcctta
     4381 gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat tgaaacatcc
     4441 gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg attagtaaaa
     4501 gccaccgcaa ataatatgaa acaaaatatc gtgatttcag ttggagtagt gttggtcttg
     4561 ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt ggttcatgaa
     4621 ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa gttgaaaggt
     4681 cggaaagaac aaaaagaagt atcagcacct acagagaaag taaaaatatc ttaagaataa
     4741 attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa actcagattt
     4801 ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt atagagtatg
     4861 ttctttataa aattaatttt cgtgtaaatt attaaaaatt taataataaa atttaaattc
     4921 ctattaagtt gtaacacatg aaattgctta aaatagtgta tcaaaaacct tgttttatta
     4981 atgttgaatt tatttgaaag gtctacagat taacatatta ttgacaaaac aatatttaaa
     5041 tagttatact tcattcaaac gtctgtttga acgaggtggt gaagttaatg actgcagaag
     5101 tatgtacaac tacttgtata cataaagaaa aagtagctag tgttaaaaat aatttaaaag
     5161 ataaagacct ttcaacttta cttgtattag gaaaatgttt tagcgattct tctaggatta
     5221 aaattttcta tgctttagaa acgtataagg agatgtgcgt ttgtgattta gcagcgatac
     5281 ttaatgctag tattgctaca acgtcacacc atttacgttt tttgaaaaag aacggaatgg
     5341 cgaaatcacg tcaatatgga aaggtagttt attactcttt agcgaatgaa gagattcttt
     5401 ccgctgtgca ggcttttcta aagttgtcag aaaatagttc tatgaaagct tagtttggag
     5461 gtagttattt tgttacaaaa cattttatca gcactcgctg tatatatttc taccagtatt
     5521 gattatttat ttatcttgct gattattttt tctcaaaacc atactaaaaa aggtctccgt
     5581 cagatttttt ttggtcaata tcttggaaca ggaatcttgg tagccataag tttgtttgca
     5641 gcatatgtgc taaattttat tcctcaagat tggatcattg gtcttctggg actaattccg
     5701 atttatttag ggattcgtgt tgcttttgtg ggcgaagagg aggaagaaga aggggaagtc
     5761 gttgagaagc taggatctag aggaacgaat cgtttgtttt ggacagtttc cttaataacg
     5821 attgcttctg gtggcgataa tttagggatt tatattcctt tctttacctc gttatctttt
     5881 tcagaaattg tcacttcttt aatcgtcttt gctatctctg ttgcggttct ttgctatatt
     5941 agttataaat ttgcgaaaat ttcttttgtc tctgaaacac tagaaaaata tgaacgaatc
     6001 attgtaccta tcgtatttat tggattaggg atttttatta tgatagaaaa tggaaccatc
     6061 caaactatat taggtttgta gagatcttat tagtaaagga agcaaacgat gagtgaaaat
     6121 aaaaagtacc atgaatggat attagaagga atcgactgtg caaattgtgc agacaaagta
     6181 gaaagaggcg tcgccaaagt tgcaggtgtt gccaatagta gtgtgaacta tatgacagaa
     6241 accttgagtt tcgaagtgac agaggaaaaa gaatatcttg ttttatccaa tgtgcaacaa
     6301 aaagtaaaaa aattggagcc tgatatcact ttaaaaaata aagtggacgg taggctgatt
     6361 gaaataaatc cggcattaat caagaaaagt aatttaaaag acgataaaaa aaactacgaa
     6421 tggatattag aaggaatcga ttgtgcaaac tgtgctgcca aagtggaaag aggagttgct
     6481 acagttcccg gcgttacaaa cagcagcgtc aattttatga cagagacact gagcttcgag
     6541 gtagcaggtg aaaataaaac tgacgtgttg gctagtgtaa aacaaacagt aggaacttta
     6601 gaacctgacg ttgttctgcg aaaaaaatcg gacggtacat tgattgaaca tggaactgac
     6661 tctaataaaa attcgacttc taacaaatcg atttctgata aagatgagac aaaccaaaag
     6721 cagacgaata gagcttatct ggccagcctt ctcggaaata aaactaaaat tacccttatt
     6781 cgtttaatac taggattagg aatattagcg acaggaatat ttctccctat ggatggacaa
     6841 gcctctcttg ggttgttcat tctggcttat ttaatcaccg gttatgacgt tgtaggacgt
     6901 gccattagaa atattttccg aggtcaagta ttcgacgaga actttctaat gaccattgcg
     6961 acgttaagtg ccttctatat tcaagagtat cccgaagctg tagcagtcat gctattttat
     7021 caagtgggag aagtattcca agatattgct atttcaaaat ctcggcgctc cattgcggac
     7081 ctaatggata ttcgacctga ttatgccaat ttagtcacag gaaacaacgt agaaaaagtt
     7141 gctcctgaag caattaagat tggaaatatt attctcattc gtccgggcga aaaagtgcca
     7201 ttggatggaa aagtagtgga aggtgcttct gcgatggaca cttctgcttt aacaggcgaa
     7261 tccgttccaa gaagcgtgaa gccggaagac actattttga gcggatttat taataaaaac
     7321 ggattgctaa aagtagttgt agaaaagccc ttttcggagt ctactgtgaa gaaaatttta
     7381 gatttggtcc aaaatgctag tggccggaaa gcacctactg aacaattcat taccaaattt
     7441 tctcgctact atactccaat tgtggttata gctgctgcct tactggcaat tgttccgcca
     7501 ttgctagtac caggcgctac ttttaatgaa tggatatata gggccagtat ctttttagtt
     7561 atttcatgcc cttgtgcttt agtcgtttcg attccagttg gtttctttgg tgggattggt
     7621 tctgcttccc gcaaggggat tttagtaaaa gaaagcaatt tcctcgaggg tttgaacgat
     7681 cttaaatacg tggtaatgga caaaacgggt acattgacaa aagggttctg ttgcaaagtt
     7741 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagtaggagg aatgacgatg
     7801 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt ggg
//
LOCUS       DACEKQ010000114         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEKQ010000114 DACEKQ010000000
VERSION     DACEKQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.120"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(14..706)
                     /locus_tag="IW671_002471"
     CDS             complement(14..706)
                     /locus_tag="IW671_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0899437.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IW671_002472"
                     /pseudo
     CDS             791..967
                     /locus_tag="IW671_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IW671_002473"
     CDS             complement(1143..1712)
                     /locus_tag="IW671_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0899438.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IW671_002474"
     CDS             complement(1786..2109)
                     /locus_tag="IW671_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899439.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IW671_002475"
     CDS             complement(2263..3075)
                     /locus_tag="IW671_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899440.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IW671_002476"
     CDS             3429..4277
                     /locus_tag="IW671_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0899441.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IW671_002477"
     CDS             4422..5363
                     /locus_tag="IW671_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0899442.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IW671_002478"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IW671_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACEKQ010000115         7623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEKQ010000115 DACEKQ010000000
VERSION     DACEKQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.121"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..576)
                     /locus_tag="IW671_002479"
     CDS             complement(133..576)
                     /locus_tag="IW671_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899443.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(664..1029)
                     /locus_tag="IW671_002480"
     CDS             complement(664..1029)
                     /locus_tag="IW671_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAQ0899444.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(1373..3025)
                     /locus_tag="IW671_002481"
     CDS             complement(1373..3025)
                     /locus_tag="IW671_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0899445.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3085..3664)
                     /locus_tag="IW671_002482"
                     /pseudo
     CDS             complement(3085..3664)
                     /locus_tag="IW671_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(4896..5612)
                     /locus_tag="IW671_002483"
     CDS             complement(4896..5612)
                     /locus_tag="IW671_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899446.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5637..6035)
                     /locus_tag="IW671_002484"
     CDS             complement(5637..6035)
                     /locus_tag="IW671_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0899447.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6123..>7623)
                     /locus_tag="IW671_002485"
     CDS             complement(6123..>7623)
                     /locus_tag="IW671_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0899448.1"
                     /translation="IDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSL
                     IGCDEPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNEL
                     KEDDGKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIK
                     TNNWFDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLP
                     AQVEYVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCS
                     DFKRYSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQI
                     EALLSVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYR
                     YELAKLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEI
                     GSDLKKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2559 a         1632 c         1116 g         2316 t
ORIGIN      
        1 aatcatttag ttttatctcc tttttaccaa ccatctacca ctaggttacg aactacctaa
       61 gtgcccacca tccgccgcgt ggaggctgtt aatcaggatg gcaaccgccc ggtagaggcg
      121 acaattatgc tatcatttta atttaagtct atataacata atactataat aatatgacaa
      181 caccaacgtt ataggaatgg aaacaaatgc cgccaaaacc aagttaggga taatttgatg
      241 aatcgtacta aatacaagaa tatggagtag atatatatca tatgagtatc ttgaaatcct
      301 aggcattatc tttctaaatg gaataacttg atacattaaa attgcaattg caccgtaacc
      361 gaagaatgca aaaatatcat taatagactc catcagtcca ccaataattt tcgatatacc
      421 agcaaaacca attccaacaa tggacgccat aatcacgaat atattattaa atcttactat
      481 ttttcttttc actaaatact ctgcaaccaa acatgccgaa agtaaattcc caaatatatt
      541 gcgcaaagca gctcccaaga actcgtatgc ttccatatcc tgtatgaaac atcataatag
      601 cccagatact aataactcag cttgcaacaa gcaatcattt cattcctatt tttctctata
      661 gttttacaat tggtatgaat aagaaataga actgaataat tgtcgaaata aaccaaaggg
      721 acccagtgac tcctcatata ctggtagaaa aattttaaat aacaaaacat gagacaaata
      781 tacttttgca gcatttaaat cttgtgtcat ataaggtagt aacactgata ccgtaattac
      841 gataatatac ggaatataaa tcttgataaa tcgtttctgt aaaaattcac ccaatgtaat
      901 aggctttttt aaatatgaca gatatagccc aaaacctgag caaaagaaga atatatgcac
      961 tcctgtatca ccaatagata ttgctttgtt cagtattgct ggcaaatacg attgctgaaa
     1021 atagtacatc aatacaatag cgaatattga aaacgccttc tcaaaactaa taatatcacg
     1081 tttatttctt tccccctcat tttattattt caatagtttt attcatattc tactcctttc
     1141 atctctattg ctgaatacat tattaaaact ctaatatctc ttatttctcc cttactgctt
     1201 tgatcggtgc ttcatcaggc tgctgtgtct ctgttaacac ttctttgcct tttcgatcca
     1261 cacagtcggc ccctttacct caattatgta aacagaaccg taaagcagga aatttttatt
     1321 aaacatagat gttgctactt cggacattga cactcagatc tttttcatag tattacttaa
     1381 acatttcagc cagttcagca gccataacag cttccattac ttcggcagct tccttgcttt
     1441 ctgcgctaac gctgatatag gtcttcagct tcggctcagt gccggaaggc cgaaccacta
     1501 cagagcagtt gccctccagc agatatttga gaacatcaga cttcggcagg ccattcagtc
     1561 ccgtgctgta atccaaggtt tcaatgacct tcttacctcc aatcatatca agacctttat
     1621 ggaattcaac cataattttc tgcatcttgg taaaacctgc cgcaccctca aactcaaagg
     1681 aatgcagggt gttcaaacag tagccatact tagcataaat ctcgttcagc ttatcaaaca
     1741 gactgatacc ctgagtcttg tagtaagaga acatctcgca gatcagataa gcggcgttga
     1801 caccatcctt gtcacggaca tagctgccgg atagatagcc gtaggattcc tcaaagccga
     1861 aaatatagct gtccaccttg cctgcctttt ccagcatacc aatctgttcg ccgatgaact
     1921 tgaagccggt caacacatcg acagtacgca cgccatagtt ggaagcgatc cgctcgccca
     1981 gatccatagt cacgatggtc ttgaccatca cagggtcagt gggcatcttg ccgtgcttaa
     2041 ttctctgaga gcatatgtag tccagaagca acagaccagt ttcgttaccg gacagcagga
     2101 cataatcccc ttctgcattc ttcacggcga tacccacacg gtcgcagtca gggtcggtag
     2161 ccagcagcag atcagcatta tacttcttgg catactccat gcccagagac atagcttcat
     2221 taatttccgg attcggataa ggacaggtcg ggaaattacc gtctggcaga cgctgctctt
     2281 cgacaacggt cacattgctg tagcccattt ctgccagagt acgggtaaca ggcaccagac
     2341 cggtaccatt caaaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca
     2401 gaaccgactg gccctttact tcttcaatga aagcagtcag aacctctggc tggatagaag
     2461 cgattctgga agcgtcgccg cccattttca catctgcaaa aatatccagc ttttcaattt
     2521 cagccagaat ctcagcggca gcctcggtgg tgatctggca gccgcctgcg ccatagacct
     2581 tatatccgtt atacttggaa ggattatggg atgcggtgat cataacaccg gcagaagcct
     2641 tcagataacg gatagcaaag gatacggtag gcacaggcag cagctccggc cacagatgca
     2701 cctgaatacc gtttgcagca aagatgtccg cggcaacctt tgcaaataca tcggacttga
     2761 ttcgggaatc atagcccacg acgacagaag gggtttcata ggtcttattc agatagtctg
     2821 acagtccctg gcttgcctta cctacggtgt gtacgttcat acgattagtg cctgcaccaa
     2881 tggtgccacg cagaccaccg gtaccaaagg acagattttt atagaaggag tcctcaatgt
     2941 ccttctcgga atatgaccgc agttcctcta caatttctgc attggcattt gtaagccaat
     3001 gataataatt tgcattatga tccatagaat ctctccaatc ttttaaaatt ctgctcattt
     3061 atttttaagg ttaggatgaa tatatcagct caaataatat ataaccccac taatgccgcc
     3121 acccaaagta ttataaaata aatctgacaa ttggaatgtc tctatgtgta aaaataactg
     3181 caaaaactct ataagacacg aacataaaaa aacaaatttt ataatctctg ataaattttt
     3241 ccaaaatctt tttctgaagc cttataccac attactagaa ttataaatgg aataaacaga
     3301 ataacatttt caaaacattc tgtagttaat ctaatctccc ctgtcgtttc attcacagag
     3361 taaatccacc aactaccaaa cacattagac aaaggattta gccaaacata tctatccatc
     3421 agtgttctaa aaagaatcat atttgtaaaa aacacaagca aaaacatttt tctaaatgag
     3481 cttgattttc tgaaataccg aatccagaca agaaaagatt ttttcaaacc taatcctgat
     3541 ttctcctcag ccatcgcaaa caagtataaa aacatagcca aaactgcaat taaaatcgaa
     3601 acccaaatat ttgatacaat gcagataaca cattaaccaa aatagaagat atgattttct
     3661 tcataaactc tcctttcatc atgcgctaaa tgagtaccat caagaaggtt tcgtaaaagg
     3721 aggtacccac tctaacatga ataacctcgg ccagcgaaaa taaaactcaa cagaattcct
     3781 atctctatgc tgctataatt tcaacaaatt tacatgtacc agttactaat ctaatataat
     3841 tttttcagca aggtaaattt cacactacac gcatacacaa aaactgtaga aattaaaatt
     3901 attatcattc cacaagtcat ttctattaat gaatttgtaa tccccaattt tgggaaaata
     3961 aaatttaaag cagctactat atacggatgt aaacaaaata ataacgtact aattcttcgc
     4021 agatgtactg ttatttttat aaatgtacta tctttaggct cagtgatact gatacaacta
     4081 ccagtgctaa tggaatggaa aatattaaca aattattgac ttctcgcaaa gctatttgat
     4141 ttatgatgaa actttcacca actaataagc atcctatagc taaaacagca cataagataa
     4201 aattacggta tttaaagacc ttattaagta tagtgttttt gtggaaggct aagtgttgcc
     4261 caataatgac gcatggtaca cctataaata atccattacg agttgttaca aacacgcttt
     4321 tataacaata tctaatatac tcgatggaat cattcccaga taatataaac caataagatt
     4381 caaatgctaa acctactacg catagcaaca aaccactgag aaactttatt ttagaaacct
     4441 ggcttttgcg tactaaccat agagcaaaag ccagtgcagg caagaaccac aggtgatagt
     4501 aactaccaat aaacagaaaa tctctgatat acaataatac atctgttttc cagctaaaat
     4561 ctgactgtac ccaattcttt aatactaatg gaatatataa tatagaccaa ataatgtaaa
     4621 ttttaagtac tctgtagatt tgtttagata atttagcgga tgtaacttca ccactacaca
     4681 acaaaaagga actgatgatg aaaaaagtag gaacggcaac cgtactcagt cctgcaacaa
     4741 taccaatact aaatgcttaa tctggagcta aatagttttg acctaaatga cgaacaacaa
     4801 ctaaaatagc cattagtatt tttagaatat ctatcgaata gtactccccc acacacgcag
     4861 ctttataatt aacattttct atgattatat tttccctatt catttaagaa cacatcgagc
     4921 tcttctggag taccgagtac gtgaactttt gatttatcta ctatactaat ggttaccttc
     4981 atgcccttct ctatcatata attatatagc ggcgcaatat atttctcatt cttttccaga
     5041 tgcctttcat ctgcataata ttcttcgtag agtgatgcat ataattttgc agaagaaaaa
     5101 taatatgcac ctaaagtaca attgttagaa attctaactt tttctcttac ttctactaca
     5161 tcaccatatt cgttcaactt ggcaaaactc caatgatcac cgtccgcatg gaaacacgga
     5221 atatgtcctt ctccagaaat atcaacgtat ttcatctcac ctggctcaac atatgtatct
     5281 atgttataaa ccataattgc attatcaggt gcgcaataag gaattgcaag catacatgta
     5341 gtggcttgtc catcagtcat atagtcaatt tcaactacct cgacattttc aattccatac
     5401 agactgcaat gttctttgat aaaaacagta gcatcatctt ccttacgcac aacaaaaaca
     5461 tacttttcta catgtgaatt ataatcaagc aagctgtcta aggaccaatc aaataaggtt
     5521 ttaccttttg cttcaatcat aaatttgggc acagtgtagc caactttacg aaagcgactg
     5581 ccaagaccag ccattgtaat aataaccgtc atcttattca ctttataaag tctcctttac
     5641 tcagatttca atttggattt tttgcaaatc tcatcgagct gatccagggt acacgataaa
     5701 aattcatccg gacgaactgt gcgatcatca acataaaacc ctttatggcc tggccaaggc
     5761 tttccataaa gaatttcatc atacggaatc tcccacttat ctagccaagc caacaagatt
     5821 tttgcagtat ttgcattgat cacacctaaa ttaccatgat aagaattcat attgcgagat
     5881 gtgaacagtg taatctttgc tccgttctcc ttatagtatc gcaacttctc caccatatta
     5941 gcatacggaa ctaaatcttc atatttctca tctttctttt tgatggggca caaagttcca
     6001 tcaatatcaa aaacaaatgc gtaatcttta aacatataac taagacatct cctttatttt
     6061 agccaggcaa tttatatgcc cggctttaca ctgatcaaat agaaaaacaa tagctcaaag
     6121 cattaacaat aaatatccgg gacaactcgt cctaaaattt ccaaaccagt tgctaacata
     6181 gccatctgat ggtttaagct ttctccatgc aaaggaatca tacttaaaaa cagcaaagcc
     6241 tcaataagtt caattttttt caaatcagaa cctatttcat ctttgaagac agagtaaaat
     6301 acttcatata aatcataatc tcgtttacga tcttgaacaa tatagtctat gattgccttc
     6361 tttggatcat attttattgt aaacaagtcc ttaattataa agtcatattt tccatcaaca
     6421 ctatgaaaca attttgccag ttcatatcta taatcaccat aaatatcaaa atctccaaac
     6481 ttacctcgcg gatctatgac tttaatgaac gaaaatgtgt tatctaccat aatattggca
     6541 aagcataagt caccatgaat aatattaaat tgcgtaatgt caaacaattc tcttggaaca
     6601 cttacgctca ataaagcttc tatctggtcc aaagatttat atctgactcc atttatttga
     6661 atatcagaag aaaagaattc tgtgaaccgg gggtcctttc tgagcttatt gaatctctga
     6721 aatgttttat cgagatacat atctttcaat gacttttgaa tattatcccc tgaaacagag
     6781 tatctcttaa agtctgagca tacaaatcgt attctattaa agatatctat ccattgtttt
     6841 tttgttaagt cactatagag aaataactcg tgaacagtat gatacgaata gtattccata
     6901 cttacatatg gattaatata cgaagtagaa tattcgaata ttctaggtct cacatattct
     6961 acctgtgctg gcaacttaag ataccactta atttctccga taaatttttc aacatcttca
     7021 ctaatttttc tcaggatgct acgatcttta tcaattgaaa tgtggttaaa ttctcttgcc
     7081 ttaacttcca atttggaatt ataatattta tctgcatgtc caatgtcaaa ccaattattt
     7141 gttttaatag agagaaattc atatcgtttg ctatactctt gtaaagcttg ataaaaacta
     7201 ttcacattta ctacatttga catcaaagct ttacgcaaac actctctaaa atactgggca
     7261 tccataattt gaaatacacc agaaaacagt ttgtgcttct ttccatcatc ttcttttaac
     7321 tcgttcttat ctaaaacaga aataatatct ccgtcttttt cttcaaagaa tgtccaagta
     7381 ttactataat agtcttcagc atagaaaaag ctgtcacatt ccaaggaata tatattatca
     7441 ttgataatgg tatcagcaaa attaataata acaggctcgt cacatcctat tagagaatca
     7501 tatatagttc ttccaagatc ccctaactct ttaagctgaa taatatttac tgttttagat
     7561 ttaatatact tatctaatcg tcttgctacc ttatccatct tttcgtaaca ggcaatatcg
     7621 ata
//
LOCUS       DACEKQ010000116         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEKQ010000116 DACEKQ010000000
VERSION     DACEKQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.122"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW671_002486"
     CDS             complement(137..427)
                     /locus_tag="IW671_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899449.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW671_002487"
     CDS             complement(545..805)
                     /locus_tag="IW671_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899450.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW671_002488"
     CDS             complement(942..1355)
                     /locus_tag="IW671_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0899451.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW671_002489"
     CDS             complement(1811..2296)
                     /locus_tag="IW671_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899452.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="IW671_002490"
     CDS             complement(2435..2680)
                     /locus_tag="IW671_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0899453.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IW671_002491"
     CDS             2873..3673
                     /locus_tag="IW671_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0899454.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IW671_002492"
     CDS             3692..5560
                     /locus_tag="IW671_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0899455.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IW671_002493"
     CDS             5553..6044
                     /locus_tag="IW671_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0899456.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IW671_002494"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IW671_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0899457.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IW671_002495"
     CDS             6604..>7598
                     /locus_tag="IW671_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0899458.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEKQ010000117         7474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEKQ010000117 DACEKQ010000000
VERSION     DACEKQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.123"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(179..475)
                     /locus_tag="IW671_002496"
     CDS             complement(179..475)
                     /locus_tag="IW671_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0899459.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            830..1186
                     /locus_tag="IW671_002497"
     CDS             830..1186
                     /locus_tag="IW671_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0899460.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1196..2095
                     /locus_tag="IW671_002498"
     CDS             1196..2095
                     /locus_tag="IW671_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0899461.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2612..3343
                     /locus_tag="IW671_002499"
     CDS             2612..3343
                     /locus_tag="IW671_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0899462.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3488..5515
                     /locus_tag="IW671_002500"
     CDS             3488..5515
                     /locus_tag="IW671_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0899463.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5623..6720
                     /locus_tag="IW671_002501"
     CDS             5623..6720
                     /locus_tag="IW671_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0899464.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            7027..>7474
                     /locus_tag="IW671_002502"
     CDS             7027..>7474
                     /locus_tag="IW671_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0899465.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2591 a         1211 c         1482 g         2190 t
ORIGIN      
        1 atgttccatc tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat
       61 tttctgtcca gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac
      121 aatgttcaat cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt
      181 aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg
      241 cgggaagctt actccaccgt tttaataatt gatagaaata actctcttct gctttgattt
      301 tatactcgct taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa
      361 tatccaatgc tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac
      421 cctcgatatt ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga
      481 ttcctccaaa gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc
      541 gtgaacaaaa agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat
      601 tttttgttat ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt
      661 ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat
      721 cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta
      781 gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga
      841 agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt
      901 attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga
      961 tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc
     1021 tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat
     1081 ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc
     1141 aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg
     1201 aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct
     1261 gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga
     1321 tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt
     1381 atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat
     1441 ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac
     1501 cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga
     1561 ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac
     1621 agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga
     1681 agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat
     1741 gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc
     1801 attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca
     1861 tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta
     1921 ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta
     1981 tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca
     2041 aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag
     2101 cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga
     2161 tccatttatc gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga
     2221 tcggatgatt tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat
     2281 aatcacaaaa aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag
     2341 tctaggcagt caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg
     2401 ctatgacagc tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca
     2461 aatcgggctg ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt
     2521 cttttatttc ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat
     2581 atgtttagta aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc
     2641 agcaatgatg ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata
     2701 cccaaaggaa tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac
     2761 tcctcctgtt gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc
     2821 aagccctgga acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac
     2881 tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg
     2941 gtctagaaaa ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa
     3001 attaagtgtc tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc
     3061 acagctgaaa tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca
     3121 aacggtgaat tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc
     3181 taaatcagga acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa
     3241 tcaagatgcg gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc
     3301 cactaaatta gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg
     3361 ttaaaaataa atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg
     3421 ctaggctaaa tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa
     3481 agatatattg aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat
     3541 ctttccgatc atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga
     3601 cgaagaaagc aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga
     3661 aagcagtgag gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga
     3721 gggttcggac aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga
     3781 tcccaatatt atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat
     3841 attggaaaat gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat
     3901 ttggtcaaat aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt
     3961 aggaaatcaa cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag
     4021 aatggctagt aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc
     4081 tactcgttct ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa
     4141 aaatagtggt tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg
     4201 acatgtcgct tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg
     4261 cgtgacggta gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg
     4321 ggacgcggag caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat
     4381 tatacctgat ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc
     4441 aacaggtact ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac
     4501 atcaacagct gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg
     4561 aaacgataag ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa
     4621 gaaaatgcag actagtatcg acctcccaca agaattaact attcccgagc atatcttgat
     4681 caatggaaat caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa
     4741 tctgacagat tattttattg ctggagatac gttaataatc gaattggaga caactttgca
     4801 ggaagtaatt cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt
     4861 gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca
     4921 tgtttattat aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg
     4981 gaaaatagga gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc
     5041 ggattcagcg aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta
     5101 cagaaaaggc aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa
     5161 aatatccagt agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga
     5221 tgaacgggct tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg
     5281 agattttgag cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg
     5341 gacatccgct gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct
     5401 tttagattct acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat
     5461 cggaaccttt gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga
     5521 agagaagaat ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg
     5581 tgtcccacag agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt
     5641 ttcttgagac caaaacaaca attatttata acactcctag ttattttagg ggcacttttt
     5701 aacaatatat cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag
     5761 aatcaaatcg acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta
     5821 gaagtaaaag cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg
     5881 aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa
     5941 gacatgagcc tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta
     6001 aaacctaaaa gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat
     6061 ggtgtattgg ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa
     6121 actaaggatc aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg
     6181 caacagacac tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa
     6241 atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat
     6301 caagtagtta ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag
     6361 gaaaaagaag gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat
     6421 ggtcaaccgc taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat
     6481 gaaaatggaa aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg
     6541 gtatttgaaa aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat
     6601 gttacgatta aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg
     6661 tttattttat tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa
     6721 ggttaagata acaaatagta aatttttatt attgtaggat ggttgggctc tttttataac
     6781 aatataaaat ataccatttt attaataaaa atgcatacta gtaaatgata taattctaat
     6841 aacaggtttg atcgcgagaa aggccaaata aaacgctctc caaaatgtta aaaaaaagac
     6901 aggacgatgt atattataca aacaagaatc gctgacaaca gtgactcgga tccaggaaat
     6961 cagttttatc cccagctagt tggtgaatgc atggaaagta atatacgtag aaagagctga
     7021 tgactaatga attatgagtt ttttttaagc aaagaagatc acagaagtta ttcattactt
     7081 aaatatttag aagcaagctc aaatttatca gagtcaatat cgaatgttca agaagagttg
     7141 tcgctatcga catttctctt gaaaaaaacg atcgataagc tgaaacatga tttacaaaag
     7201 ttcgatctag agaaaaattt gcgagtaacc gtcagtgaat tggatatatg tctggaaatt
     7261 aatggaaaat gttctagcaa agctctgtta tctcgttata taacggattc tttatctatg
     7321 aaaatggtat tggctttctt tcaggaaaaa tatatctcag tagaaaattt tgctaaacag
     7381 catcacgtga gttattctgt ggcttataaa gtattacaaa gactaaaaag aaatctaaaa
     7441 aaatatcaaa ttttttttga aaagagaaag ttga
//
LOCUS       DACEKQ010000118         6858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEKQ010000118 DACEKQ010000000
VERSION     DACEKQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.124"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW671_002503"
     CDS             127..579
                     /locus_tag="IW671_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899466.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW671_002504"
     CDS             593..1282
                     /locus_tag="IW671_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899467.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IW671_002505"
     CDS             1407..3758
                     /locus_tag="IW671_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0899468.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IW671_002506"
     CDS             3838..4233
                     /locus_tag="IW671_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899469.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IW671_002507"
     CDS             4558..5175
                     /locus_tag="IW671_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899470.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IW671_002508"
     CDS             5216..5515
                     /locus_tag="IW671_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899471.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IW671_002509"
     CDS             5549..5716
                     /locus_tag="IW671_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0899472.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IW671_002510"
     CDS             complement(5761..6324)
                     /locus_tag="IW671_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899473.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..6858)
                     /locus_tag="IW671_002511"
                     /pseudo
     CDS             complement(6352..6858)
                     /locus_tag="IW671_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2310 a          989 c         1355 g         2204 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactattta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc ttcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataata
//
LOCUS       DACEKQ010000119         6843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEKQ010000119 DACEKQ010000000
VERSION     DACEKQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.125"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1643)
                     /locus_tag="IW671_002512"
     CDS             complement(<1..1643)
                     /locus_tag="IW671_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0899474.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDEC"
     gene            complement(1806..2399)
                     /locus_tag="IW671_002513"
     CDS             complement(1806..2399)
                     /locus_tag="IW671_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899475.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2400..2732)
                     /locus_tag="IW671_002514"
     CDS             complement(2400..2732)
                     /locus_tag="IW671_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899476.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2782..5154)
                     /locus_tag="IW671_002515"
     CDS             complement(2782..5154)
                     /locus_tag="IW671_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899477.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5151..>6843)
                     /locus_tag="IW671_002516"
     CDS             complement(5151..>6843)
                     /locus_tag="IW671_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0899478.1"
                     /translation="QKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDP
                     DNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIA
                     KMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGYRGFCEI
                     NFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKE
                     AVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQC
                     VKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGE
                     QNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDS
                     DRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNP
                     SWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMHDQIEEEAKENIFIKIV
                     QSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKAVKE"
BASE COUNT         1905 a         1395 c         1095 g         2448 t
ORIGIN      
        1 tggcactcat caatcatcgc caagaagcgt ttgaagtctt gttcttccat ttgtttttct
       61 ttaagtaaac gtcgttgtct tcttccttga atcaatgcct gattccaaat caacgtcata
      121 gccatgtaca tttgacattg ttgtacccct cgatctaagc ctgtaataga gtcaatgtca
      181 aaaaagacca actgttgttg gtcaaggtgt cgaatcgtac tgtgaccatc aaaaatcatg
      241 ccatacgtct cacataagga ttctacgatg gattccattt cttcttttcg attctttttc
      301 tcttgggaag cacctggtaa ttccagttgt cctaaaaagt ctaaaaattc tttgaacgtc
      361 ggataagctt ctggatcttt ccctgtgact tgcgtctcag tatttgaacg cgaatttgag
      421 gaagtaaagc cgcgtggtac tgttccatac tcttcataaa aacgatttaa gtaggtctta
      481 aattctgctt tcatcgttcc ttttagctga ggactcacta actgtacctg atagactact
      541 ttgtctaagt gtgtttgata ggacgctgct tcattgatct tcaaatcttt gactgatcca
      601 tcgtcatata aactggatgc ttcatcatac gtccgtgtgg caaatatttc taaaggattg
      661 atcatcccgt cagacccgtc taagcgcacc ataatgccgt tatattcctt taagaagggg
      721 ataaagtctt tccccttttc aaagccccaa ataaagtctc ctgcagctaa atgggcttcc
      781 gcgatcattt taagaacggt tgatttacca gctcccatca ttcctagaag caaggtatta
      841 gtagacaacc gcacactatt tttatatgtc gcattccaga caaatgggcc acctgtcatg
      901 gtttccccat attgagtccc tcctggatct tttagcgttt ggtggtggaa cgggaatcct
      961 ccgccaatat ttaacgatgg tagtgattgc cctttgatat ttccaaacca ttcagcttgt
     1021 tcatcgaaca tggtaaacat cgattgccaa tctcttttcg ttttgtgcag ataggtcact
     1081 gcacgataat ccatcccttt taaattttca atcagttcct gcgctcgttc atctacttct
     1141 tgaatcgtgt ttccgtaaac atgtactctc actttgactt gcttagaaat ttccccatta
     1201 gaagtcaatt tacgagcata ttccgctagt tccaggtatt cttctgcgct tttatttcgg
     1261 tcagtcgctt ttcgttcatt atctgatcga tcatttaatt catctaaggc acgattgatt
     1321 tgatcaatca ctttttgctt ttcatctgtg ctaacatcga gtgtggccac tgtatgagga
     1381 ttgttcatga gattggccaa ccaaaaaagc gtaggatttt ttgtgtagtg ataaactgtc
     1441 acacatcgtg aataaccatt accatacgtc acatattctt catcaaaatt taacccacct
     1501 tgtggcatca cttgttttaa aaaatctaaa tcataccctt tggatttcaa aatttctatt
     1561 tcccgatttc ctagttgctt taatggatca agtacttttt gatcattttg tggtgaaaaa
     1621 gctgttttaa ttgcttctaa catgtacagt cacctcttct tgtttgattc tttacttggt
     1681 taaaagattg cccttttccc aacttaccca ggcttaaagt gtgaggaaat tttctagtgg
     1741 tcccttcctt cggaacatct ttttctaacc atatcaatgg atatttcatt ctctcttctc
     1801 cattcctact catcattcat attgttcatt cgatacagaa tcttgatcaa ttcttttttg
     1861 tagagtggtt ttaaccctaa aatgccacta gatcgcatta actgacggtt tctggtttgt
     1921 aaatccttga ttgtctttcc ttcaatgacg aaaaagaagc ttaagtcagg acgttcttgt
     1981 tctgctcgtt tcaattcctg gatttcttcg actaatttct tttcatattt ttttaatcgt
     2041 tgtagatttc ttcctaagga ttgttccata cgattcttat ctaataactg ccgcttttgc
     2101 acgatttgtt ccgttagatc cgtctctgta gtcaaactaa ctaacttaat tgttggcaca
     2161 tacgtacgca tgacggtggt caactgattc attcgttcca gtttctcttg ttcatttaat
     2221 ccctccacgt cagtggaaac gacacgataa taacgttgaa aatgatcttc tttagtaact
     2281 aagactccct cttctgtaca ggatttataa ggaaataaat ccaagataga tcgttcttct
     2341 ggttttccaa actgcaaaat agatttctta tcaactgaat tttgtccttt gactcgcatt
     2401 tattcatcct ccttatacgt accatgataa tttcggttag gatctaacgc atgataatgg
     2461 ttcttgtccg caaaaaaaat agactcgata atcgcaaaaa tttgtcgttg acctgggtta
     2521 ttatagggtc gccaaacaat cactaacgct atcgcaatcc ataagccata attcaagagt
     2581 gcaaaaaaga gtggtaagtg gacaagatta ttgaccattt tcgcccaaaa aaagcagcca
     2641 ccaagcgtta atagatctaa aaagaaaaaa gcgaaaaact tccattctcc actaaatctt
     2701 ggagggtttt tcatgtttat atatttatcc ataattgatt tctccttttc atataaagaa
     2761 aaggcctaac ttgtaggcct tttatggttt gatatcctgt ttgatatccc tcaatagttg
     2821 ttctttcaat gcattgttcc tgtttttggt atctcttgtc acatttattt ttgtcggtgc
     2881 agtaaatgcc acatttgctg gcttgtaacc tgtatgaacg ttttgaatag gttttggttc
     2941 agcttctctt gtcacaatat tttgaacgga cgcattctct gtctcaccac tttcagataa
     3001 attgtaagaa acagttgtca aattcccatt ttcaggcaaa cgacttgatt tcatttgaac
     3061 agtacctgca ggtgattgat gaacaaagct ttgatcattc tctttgaatt gattcaaacg
     3121 actatctttc attggtaaat tttgtgtctt aggcaaacca attttcgttg atttttcagt
     3181 tccaacttcg atgccattat catttggcag atcgatggaa tcatcatccg tttgtacttc
     3241 ttgatttcgt tttggtaaat ctgaaatgcg cttttcattt tcagaattaa tgttagattt
     3301 ataacgatat tgttctggat ctgtttttgg taattgacca ttttgtcctt cttctgaatt
     3361 ttcgttagaa tgcaactcat cttggtgatc tttggctgaa ggaatcgctg caccttcttg
     3421 attttctttt tcggttgttg tattagcttt tactgctgca ttgacttgtc cttcaccata
     3481 cgcatctttt agtccttctt tgatttctga agctttttct ttttgttttt ctagtgtttc
     3541 ggatggattc ttcacagctt cagccaattc tttagctctc gtgccgtcta atttctcttc
     3601 taaggtgtct ttggcttttt ctgtaagatc ttcagctgtt ccttttaacc cacgttcatt
     3661 gacgtaacca agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc
     3721 ggaaactgtt ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc
     3781 gttatcaggg gtaacatctt ttcgattacc aaatccattt ttacgaatat catttaaatt
     3841 gcttaaacca ctcttaccag cacttgcaag gtttttagcc ccgctaccca catttttcgc
     3901 aacatttcct aaggcaaaca tgctaagaag agaattgctg ccgtttttca gactcgtatc
     3961 aacgccgaaa attttcgtaa ctgattgaga gcctttgaat agcgcaatgg ttaatacaat
     4021 taaagcgaca acatacaaga atccattgat ctttcctgcc gcatgcaaat caccgatacc
     4081 tgaaagcatt aatgtataga ttcgtaattc taaaatcgta aatgccaata acaagaagga
     4141 ttgagaaata tcttctatag cacttttcat ccgttgtcct gtctcaaagt ccgttgacgc
     4201 ggcaatcact aaatagaatt ttttaaatac cagctcaata atgcaagtaa caatcgtaaa
     4261 caagataaat aaataggcta ccgacaaaga agattcacca gctaaaataa tacctggact
     4321 actaatgtat cggcgatagc ctgaagtata cacatatttt gctaaacctt catcactaat
     4381 ttgaacaggg atttctttcc cattatcgtc taactctagt cgatactgta atgcctttaa
     4441 ccgatctttt tgtactgtac taatatcact tgattcagac attttcttag cttgatcagg
     4501 tgtaataatc ccattgatat tcgctgtttt aaaatttttc gccgtgagag catttctttt
     4561 gtctgttggt atgtgttcta ctttgttcgg atcattttct agtaacgtac tcacatcaat
     4621 cacattattt tgaataattt gaaaagcagg tgaaccagtt ttatctgctt tatttgctgc
     4681 agtcacgtct ccgtaaaagt ttttactaat atttagccct tcatcgatta aagaactacc
     4741 accaaaaatc aaaatcatgg ccataaatag attaaccaag atatttttga tttttggtgg
     4801 atgtttactc ataaaaaatt ttaagcctaa ataaattaat gtgacgacac aaaaaagagc
     4861 caatacctta ctaatcaaac tttgaaacag ttcattaaca ccagcggcgt tcaataagtc
     4921 ttttaaatca aagctttgat aaattgtttt ttctagtaaa gtattgagat tatataaccc
     4981 tttaatcaaa ccccataaaa aagtattaca aatccaactg aatacatctt gatactgcag
     5041 ataattacca taatcttgta agactttcgt tacggttttg ggatctgcat ccccatcccc
     5101 aaataagcct gaccaccagt cggcaatgcc ggaaaatatt cctccaatga tcattctttc
     5161 accgcctttt cagcttctag caaaagtcgt ttccttataa cctctgtaat ttcttcattt
     5221 tgacgcaatt tttcaaggta ttcttgcaaa cgatctattt gcttctcttc ataaaattga
     5281 ataagtaact tttgtgccgt tgagttgtct aaagattgaa cgattttaat aaaaatgttt
     5341 tctttagctt cctcttctat ttgatcgtgc atattgtgat aaagtcgacg tgcatagata
     5401 tcaggtagaa aataatcgta atcaatttgt aaagccttaa gatccaagtc cgcatgtgta
     5461 caatgtatgt ctatatcatt gatatcccac gatggattga aatcttcaat atattgataa
     5521 gcataaggaa gattcgtttc cccatgatta aaaataggat acggccgtat tttatgacct
     5581 ttcaaatctt gtcgttttaa aggagcgagt acaatcgttt caccttctaa aaaagtagaa
     5641 attctttcag gagttaagat gcgatctgaa tcaacattgc gattgatatg agtattcata
     5701 cctaatgaat tgatggtact gttctcttgt tcaaccgtct tattccccac ttttttggaa
     5761 aattcttcta tggtattatc gtctgtcgac ataatataaa tatgtgtctg acaattttct
     5821 ttgataatcg ctgcgttttg ttccccatac ttcgcataaa gttgctgata ggattggata
     5881 aacaaatccc acaacatgtt tctaccagct gtgactgtca tgattccttc catattatcc
     5941 attggtggca tattcccaaa ttcatctaaa ataaaatgaa tcctacggtg acatttatct
     6001 cctttagtct taacacattg cgtagataat tctttataca actgactaag aaaaatacta
     6061 acaatgatat ggttagatgg atcataatca ggtgtgagca taaaaatagc cattggttta
     6121 tcggtatagt gcatccgaat agactctact aataagttac ttttatcctc atctaatgtt
     6181 agaacggctt ctttttctaa ttctggcaaa tgggtttctc ctcgtttttt tcgataaacg
     6241 atatttccct cttcgtcttt caatactctt tgagaaaact cataccaagc aacatattct
     6301 tttcctcgat gatcgtattt taagcaaaca tgatctccct gttctaaatg acaatcaaag
     6361 ttaatttcac aaaaacctct atagcctact ttgacacgtt cctcatgaaa tttttttcct
     6421 tgatgataaa actctacaac aattcgctgg tttaacagac tctcgtgtag tttgagatcc
     6481 aaatacttcg gaaaaccaat ggacttataa tcaatggttg attgagaagt cattttcgca
     6541 attttcgtca gtgtaaatgg atttagtcct gtgatcgctg tcgatagaat gttgcctttc
     6601 gctttatcag aaccaatttt tgttgcccca tattgttttt ttgctatgtt tccaggtggt
     6661 aactgattca taaattcatc taaagcattg agctgcttga attcattatc aggatcttgg
     6721 taattaaaac caccaatctc agaaagaagt tcgacgagat tattcggtgt taatttggag
     6781 taatcccctg tctgatccgc ttgttccaat aacgcaaaaa taagcgcatt gacagctttt
     6841 tgc
//
LOCUS       DACEKQ010000120         6820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEKQ010000120 DACEKQ010000000
VERSION     DACEKQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.126"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(19..1575)
                     /gene="rny"
                     /locus_tag="IW671_002517"
     CDS             complement(19..1575)
                     /gene="rny"
                     /locus_tag="IW671_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0899479.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1986..3035)
                     /gene="recA"
                     /locus_tag="IW671_002518"
     CDS             complement(1986..3035)
                     /gene="recA"
                     /locus_tag="IW671_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0899480.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3144..4385)
                     /locus_tag="IW671_002519"
     CDS             complement(3144..4385)
                     /locus_tag="IW671_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0899481.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4625..5203)
                     /gene="pgsA"
                     /locus_tag="IW671_002520"
     CDS             complement(4625..5203)
                     /gene="pgsA"
                     /locus_tag="IW671_002520"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0899482.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5412..6296)
                     /locus_tag="IW671_002521"
     CDS             complement(5412..6296)
                     /locus_tag="IW671_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899483.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         1807 a         1545 c         1207 g         2261 t
ORIGIN      
        1 cttcttttgt attttttatt atttagcata atcagttgca cgagtttccc gaataacgat
       61 tactttgatg tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac
      121 taaacgaaca gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg
      181 tccagcttga actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag
      241 acttcttaaa cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc
      301 tgagagcgca tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc
      361 atgatgagaa gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc
      421 cgcaccgatc tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg
      481 gagcaacccg gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc
      541 agctaatttt gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa
      601 tcgcagacgt ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa
      661 cgctgcctct tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac
      721 catttcttcg atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat
      781 ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc
      841 atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg
      901 tccaatgata cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc
      961 tgaaacttga tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg
     1021 atctgcttct tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt
     1081 cagctcttct tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc
     1141 aattttttct agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc
     1201 ttcaattaat tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg
     1261 tttttctaac gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc
     1321 ttgagatttt aattctaact tactttctct cagctcactt tcaacttcag aacggtattt
     1381 ctgattttct tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt
     1441 tgcactatcg atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg
     1501 tgactttgcg accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag
     1561 aatgttgaat accataaaat ccacctccat actatctttt tcatttttat catctataca
     1621 gcttctatga taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa
     1681 aaacatgcac aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa
     1741 gaaacggctt tctacttatt cattaaacga tggtttttaa aataaatgac actagtttca
     1801 tataggtaaa cctttaaatt gagaaacttt tctattaaat tattctttgt agatgaacag
     1861 atatttttat tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat
     1921 aaaaaaacgg caggtcaagg agagtaccta ctcttcagac cagccgtttt tgtttatcat
     1981 aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg
     2041 acttcttcac caataccata agctgctcta actagagcag aaacttctgc catcatttca
     2101 ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta
     2161 taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt
     2221 tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga
     2281 ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct
     2341 tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg
     2401 cgtcctccag gagtgatttc tggattccca aacatcacgc ctactttttc acggatttgg
     2461 ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct
     2521 tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct
     2581 gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat
     2641 actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt
     2701 tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca
     2761 atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt
     2821 gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg
     2881 acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg
     2941 attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt
     3001 gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca
     3061 aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa
     3121 aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat
     3181 ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc
     3241 gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct
     3301 aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca
     3361 cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat
     3421 agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg
     3481 cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct
     3541 gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact
     3601 tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt
     3661 ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc
     3721 agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc
     3781 tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa
     3841 actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt
     3901 tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta
     3961 tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct
     4021 tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa
     4081 tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc
     4141 gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg
     4201 agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct
     4261 acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg
     4321 gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct
     4381 ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa
     4441 aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg
     4501 ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt
     4561 ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg
     4621 atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat
     4681 agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc
     4741 tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga
     4801 cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc
     4861 ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt
     4921 ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa
     4981 acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg
     5041 tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt
     5101 ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga
     5161 tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct
     5221 tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga
     5281 atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg
     5341 cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact
     5401 aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg
     5461 ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct
     5521 gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca
     5581 tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca
     5641 cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg
     5701 atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt
     5761 gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac
     5821 tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct
     5881 tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct
     5941 aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt
     6001 gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat
     6061 ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg
     6121 tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg
     6181 tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat
     6241 atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt
     6301 tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat
     6361 gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct
     6421 cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga
     6481 acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt
     6541 tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc
     6601 cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt
     6661 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt
     6721 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca
     6781 cggtgttcca gaagtagctt cttcggcaat aagccgaaat
//
LOCUS       DACEKQ010000121         6750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEKQ010000121 DACEKQ010000000
VERSION     DACEKQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.127"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..395
                     /locus_tag="IW671_002522"
     CDS             168..395
                     /locus_tag="IW671_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0899484.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            411..1577
                     /locus_tag="IW671_002523"
     CDS             411..1577
                     /locus_tag="IW671_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0899485.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1812..2855
                     /gene="hrcA"
                     /locus_tag="IW671_002524"
     CDS             1812..2855
                     /gene="hrcA"
                     /locus_tag="IW671_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0899486.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2949..3512
                     /gene="grpE"
                     /locus_tag="IW671_002525"
     CDS             2949..3512
                     /gene="grpE"
                     /locus_tag="IW671_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0899487.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3563..5392
                     /gene="dnaK"
                     /locus_tag="IW671_002526"
     CDS             3563..5392
                     /gene="dnaK"
                     /locus_tag="IW671_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0899488.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5543..6709
                     /gene="dnaJ"
                     /locus_tag="IW671_002527"
     CDS             5543..6709
                     /gene="dnaJ"
                     /locus_tag="IW671_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0899489.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2307 a         1160 c         1526 g         1757 t
ORIGIN      
        1 aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat catccaagaa
       61 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
      121 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
      181 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
      241 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
      301 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
      361 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
      421 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
      481 tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta gatgagtatg
      541 tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca gcgaatttgg
      601 aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa cttgatcgac
      661 tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt actgtagaag
      721 cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat ggcgtcaatc
      781 gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt ggacgtaaac
      841 atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat tttaccaatg
      901 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat cgggatactt
      961 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
     1021 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
     1081 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
     1141 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
     1201 acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
     1261 accgcaacaa aggaccgatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
     1321 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
     1381 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
     1441 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
     1501 aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg tttgagcgct
     1561 ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga aaagaaaaag
     1621 tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta catcaattat
     1681 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
     1741 aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag tgctaatgag
     1801 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
     1861 atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga gtcaaggcca
     1921 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
     1981 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
     2041 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
     2101 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
     2161 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
     2221 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
     2281 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
     2341 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
     2401 gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg atgtctcagt
     2461 tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt ggacaaatga
     2521 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
     2581 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
     2641 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
     2701 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
     2761 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
     2821 attattaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
     2881 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
     2941 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
     3001 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
     3061 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataga tacttgcggg
     3121 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
     3181 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
     3241 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
     3301 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
     3361 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
     3421 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
     3481 taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
     3541 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
     3601 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
     3661 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
     3721 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
     3781 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
     3841 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
     3901 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
     3961 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
     4021 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
     4081 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
     4141 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
     4201 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
     4261 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
     4321 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
     4381 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
     4441 agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt tagtcggtgg
     4501 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
     4561 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
     4621 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
     4681 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
     4741 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
     4801 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
     4861 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
     4921 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
     4981 atcttcttca ggtctaacag atgaagaaat cgaacgtatg gtgaaagatg cagaagcaaa
     5041 cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagacgcatt
     5101 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
     5161 gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa acaacatcga
     5221 ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
     5281 atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc aaggcggagc
     5341 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
     5401 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
     5461 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
     5521 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
     5581 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
     5641 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
     5701 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg ggcatgcagg
     5761 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt
     5821 ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg
     5881 tcgatcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt
     5941 gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat
     6001 ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta cttgtcataa
     6061 atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac gagtgatgag
     6121 ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac cttgtccaac
     6181 ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg
     6241 tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg
     6301 accatttggt gatttgtatg tggtctttcg tgtagaagaa agtgatatct ttgatcgtga
     6361 aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt tgggggatga
     6421 agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac
     6481 aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg
     6541 cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga
     6601 agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg
     6661 tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta
     6721 aaaatataaa gtagacttgc ccaaattttc
//
LOCUS       DACEKQ010000122         6672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEKQ010000122 DACEKQ010000000
VERSION     DACEKQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.129"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            70..153
                     /locus_tag="IW671_002528"
     CDS             70..153
                     /locus_tag="IW671_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0899490.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="IW671_002529"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="IW671_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0899491.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="IW671_002530"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="IW671_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1608..2273
                     /locus_tag="IW671_002531"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="IW671_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="IW671_002532"
     CDS             2309..2554
                     /locus_tag="IW671_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0899492.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="IW671_002533"
     CDS             2657..3526
                     /locus_tag="IW671_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0899493.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="IW671_002534"
     CDS             3507..4241
                     /locus_tag="IW671_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0899494.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5186
                     /gene="ant(6)"
                     /locus_tag="IW671_002535"
                     /pseudo
     CDS             4274..5186
                     /gene="ant(6)"
                     /locus_tag="IW671_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5183..5725
                     /gene="sat4"
                     /locus_tag="IW671_002536"
     CDS             5183..5725
                     /gene="sat4"
                     /locus_tag="IW671_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0899495.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5818..6612
                     /locus_tag="IW671_002537"
                     /note="aph(3')-IIIa"
     CDS             5818..6612
                     /locus_tag="IW671_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0899496.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2339 a         1004 c         1393 g         1936 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     4381 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     4441 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     4501 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     4561 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     4621 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     4681 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     4741 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     4801 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     4861 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     4921 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     4981 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     5041 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     5101 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     5161 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     5221 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     5281 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     5341 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     5401 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     5461 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     5521 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     5581 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     5641 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     5701 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     5761 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     5821 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     5881 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     5941 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6001 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6061 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6121 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6181 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgattagcc
     6241 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     6301 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6361 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     6421 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     6481 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     6541 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     6601 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     6661 atctaatctt tt
//
LOCUS       DACEKQ010000123         6625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEKQ010000123 DACEKQ010000000
VERSION     DACEKQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.130"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(123..647)
                     /locus_tag="IW671_002538"
     CDS             complement(123..647)
                     /locus_tag="IW671_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899497.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW671_002539"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW671_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0899498.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW671_002540"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW671_002540"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0899499.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..3049)
                     /locus_tag="IW671_002541"
     CDS             complement(2369..3049)
                     /locus_tag="IW671_002541"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0899500.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3175..4044
                     /locus_tag="IW671_002542"
     CDS             3175..4044
                     /locus_tag="IW671_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0899501.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4070..4585
                     /locus_tag="IW671_002543"
     CDS             4070..4585
                     /locus_tag="IW671_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899502.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4632..5195)
                     /locus_tag="IW671_002544"
     CDS             complement(4632..5195)
                     /locus_tag="IW671_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899503.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5250..5429)
                     /locus_tag="IW671_002545"
     CDS             complement(5250..5429)
                     /locus_tag="IW671_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899504.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5506..6015)
                     /locus_tag="IW671_002546"
     CDS             complement(5506..6015)
                     /locus_tag="IW671_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899505.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6111..6491)
                     /locus_tag="IW671_002547"
     CDS             complement(6111..6491)
                     /locus_tag="IW671_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0899506.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1862 a         1397 c         1175 g         2191 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     2881 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     2941 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     3001 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     3061 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     3121 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     3181 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     3241 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     3301 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     3361 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     3421 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     3481 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     3541 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     3601 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     3661 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     3721 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     3781 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     3841 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     3901 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     3961 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     4021 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     4081 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     4141 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     4201 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     4261 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     4321 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     4381 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     4441 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     4501 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     4561 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     4621 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     4681 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     4741 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     4801 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     4861 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     4921 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     4981 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     5041 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     5101 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     5161 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     5221 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     5281 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     5341 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     5401 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     5461 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     5521 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     5581 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     5641 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     5701 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     5761 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     5821 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     5881 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     5941 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     6001 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     6061 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     6121 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     6181 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     6241 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     6301 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     6361 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     6421 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     6481 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     6541 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     6601 ggcaaaaaaa acagaaagta ggtcc
//
LOCUS       DACEKQ010000124         6458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEKQ010000124 DACEKQ010000000
VERSION     DACEKQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.132"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..560)
                     /locus_tag="IW671_002548"
     CDS             complement(105..560)
                     /locus_tag="IW671_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899507.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IW671_002549"
     CDS             complement(714..1922)
                     /locus_tag="IW671_002549"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0899508.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LYFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IW671_002550"
     CDS             complement(2034..2366)
                     /locus_tag="IW671_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0899509.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IW671_002551"
     CDS             2514..3389
                     /locus_tag="IW671_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0899510.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IW671_002552"
     CDS             complement(3471..4052)
                     /locus_tag="IW671_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0899511.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IW671_002553"
     CDS             complement(4058..5317)
                     /locus_tag="IW671_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0899512.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IW671_002554"
     CDS             complement(5319..6365)
                     /locus_tag="IW671_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899513.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1797 a         1337 c         1102 g         2222 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atacaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcacttgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaa
//
LOCUS       DACEKQ010000125         6207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEKQ010000125 DACEKQ010000000
VERSION     DACEKQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.133"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..709
                     /locus_tag="IW671_002555"
     CDS             191..709
                     /locus_tag="IW671_002555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0899514.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="IW671_002556"
     CDS             837..1520
                     /locus_tag="IW671_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0899515.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="IW671_002557"
     tRNA            1829..1902
                     /locus_tag="IW671_002557"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="IW671_002558"
     CDS             2061..2420
                     /locus_tag="IW671_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0899516.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="IW671_002559"
     CDS             2543..4174
                     /locus_tag="IW671_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0899517.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="IW671_002560"
     CDS             complement(4236..4877)
                     /locus_tag="IW671_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0899518.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="IW671_002561"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="IW671_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0899519.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="IW671_002562"
     CDS             5494..5838
                     /locus_tag="IW671_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0899520.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="IW671_002563"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="IW671_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0899521.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1955 a         1022 c         1313 g         1917 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgct
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
     6181 ttgtcacagc cttttttgtt tataaaa
//
LOCUS       DACEKQ010000126         6182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEKQ010000126 DACEKQ010000000
VERSION     DACEKQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.134"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW671_002564"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW671_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0899522.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IW671_002565"
     CDS             complement(2203..4389)
                     /locus_tag="IW671_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0899523.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW671_002566"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW671_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0899524.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IW671_002567"
     CDS             complement(4611..5051)
                     /locus_tag="IW671_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0899525.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IW671_002568"
     CDS             complement(5125..5664)
                     /locus_tag="IW671_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0899526.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1602 a         1390 c         1090 g         2100 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aa
//
LOCUS       DACEKQ010000127         5999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEKQ010000127 DACEKQ010000000
VERSION     DACEKQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.135"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..308)
                     /locus_tag="IW671_002569"
                     /pseudo
     CDS             complement(1..308)
                     /locus_tag="IW671_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            329..1531
                     /locus_tag="IW671_002570"
     CDS             329..1531
                     /locus_tag="IW671_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAQ0899527.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2264..3232
                     /locus_tag="IW671_002571"
     CDS             2264..3232
                     /locus_tag="IW671_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899528.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3243..4058
                     /locus_tag="IW671_002572"
     CDS             3243..4058
                     /locus_tag="IW671_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0899529.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4126..4836
                     /locus_tag="IW671_002573"
     CDS             4126..4836
                     /locus_tag="IW671_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0899530.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4872..>5999
                     /locus_tag="IW671_002574"
     CDS             4872..>5999
                     /locus_tag="IW671_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0899531.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2054 a          902 c         1219 g         1824 t
ORIGIN      
        1 tcagagtgtg aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga
       61 ttgatgtgtt ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagaccgtt
      121 ttaagcaact gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg
      181 gcctttctag gaaagcgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg
      241 gtcgtaaata gctgattggt tgtcccatca acacaaataa aactcatgga accagaagtg
      301 gcaacattgt gtacttttct ttgcaatctt gtgtacaaaa ctattgcaaa acacagcgca
      361 taagaatcct tacaaaacag tagatgaagc aacccaatta gatcttgtta tagttgttaa
      421 tcagctctta acgggttacg attcaaaatg ggtcaacacc ctatacttag ataagtcact
      481 tgaatatcag aacataattc aggctttttc acgcacaaat cgtcttaatg gtcctgaaaa
      541 accgttcgga accattcgtt attatcgtaa agcacatacg atggaacaga acattgaaga
      601 ggctattgag ttatactcag gcaaacaagc aaaagggtta tttgttgaaa aattatataa
      661 taacttagaa aagttgaacg atatattcga agaaattaag tccgtattta atatttctgg
      721 catagaaaac ttcagtcatt taccggaggt tccaggggaa attaagcaat ttgtaaatct
      781 ttataatcaa ctaatccatt gttatgaatc agcgatgata cagggattca catgggcaaa
      841 atcgacttat gaaatccagg attatgaacc tataacagta gtaatcacag aatatgaatt
      901 taatatcttg gcacaacgtt acagcgagat agagagagaa cctagagacg gaaatgatag
      961 tatagcgtat gacattgatt accaattacc tgaaggtagt cgtagtcgta ttaattatga
     1021 gtatttaaac acaaacttca aaaaatacat gaatataaaa gaagaacgtg attcgaattt
     1081 aattcaatca ttgttaaacg acttacatcg cacatttgcg caattaccag tggagttaca
     1141 gccatatgct gagatgattc taaatgatgt aaataatggg gaattagtta tcaaagaagg
     1201 atgggaattt actgattacc tcaatgaata tcagcttagt gaagaggacg attttatcca
     1261 agatttagtg gattcaacca atattaatga ggcattatta cgtgagatgt tggatttacg
     1321 cttaaccgag gtaaacatta atgaatatag tcgatttgat aaattaaaat caagtgttaa
     1381 cgtaggtcat ttcagtggat atatcgagaa aaatttaggt aaaatggtaa taccaatcaa
     1441 ggtaaatatg ctaattgaca gattattgcg tgcattttta ttagaagatg tattcgatgt
     1501 tacagagtac ataaagaatt actttaacta gagtacacct acatcttata gtacataaaa
     1561 aaattcatct tttttagttt aataattcac caaaaaggtg agtaatgcca tttttgaaaa
     1621 ttgagcttgt tttatgtcta catctgtatc cgtatataat ccaaaggatt ataaactcag
     1681 aagagtcttt attttttctg aatataacta ctgtatcaaa aacaatcgat tatgagacaa
     1741 aacaaaatga cctcttttat ttaagaaagg aggtcatttt ttgtatcata aattgtatta
     1801 aaatcaatgt atcacttttt aatcaatttt aagaccataa attgcgtact aaaagaaatt
     1861 ctgcaattca aaataaagca ttttaagcca ttttcaaatt taagtgcacc catctacccg
     1921 tcaaatcact ttattttcca ctccagtagt ttcctaattc aactgtgtgc aaaacgattt
     1981 cttctaaaca aatcaagaca aacttataaa ccttcatccc agcaccataa ttatttaaat
     2041 cataggcatt tttcgaaatt agtatatgta ctcaagtttc actgcagatt atttatattt
     2101 tttagagaaa taaatttttc ctattgacaa acacaaagaa agcggataca atacaattat
     2161 gtcacatgac gttatatgac gttatgtaat atctttacat ttaatcagtt acttttattt
     2221 tggataaaat taacattttt aaaattttaa ggaggaaatg ttgatggatg tagcaaaaac
     2281 ggttaacgca attgtagaaa aaaatgatgg agagatttct ggaatttatt tggtggcttg
     2341 tggcggttcg ctggtagata tgtatgtctc agattatttt cttaaagccg aagcatcgaa
     2401 gtgtattaca ggcttatata cagcgaacga atttaaccat gtaaccccaa agcaattaga
     2461 tgataaatct gttgtcattc tatgttctca tggaggaaat acagcagaaa cggtaaaagc
     2521 aggagaagtc gctcaagcta gaggagctgt gactgttggt ctgactcata ataaaactgc
     2581 ggatttatta aaggtttctg atcatagttt cctttacgaa tggggagatg atgcaaaaat
     2641 agtcaataat cctatggcca tcattcttga tttaactgta tctttggtca atgttaccga
     2701 aacatttgaa cattacggta aattcagaga aggcatgaag gttatcgata ccgtaattga
     2761 aaatggcatt aagcaggtgc aggatcgtat taaagtgttt gctgataagt acaaagatga
     2821 aacaatgttt tatatcttag gtagtggacc atcatatgga catgcttatg gtttttcaat
     2881 ttgctctttg atggaaatgc aatggcttga ttcaacttct gttcattctg gagaattctt
     2941 ccatggacct tttgaagtaa cagacaaaga tacgaatttt attctcttga tgagcattgg
     3001 acgcactcgt gctttagatg aacgggcaaa acgattcctt gataaatatg cagaaagagt
     3061 agaaatcgtt gatgcaaagg aactaggatt ggatatgata tcagatgaag tttctgaatt
     3121 cttcaatccc attttattct acagtattct ttcagaatat cgttcagctt tagcagatgc
     3181 acgcaatcat gatttagatg ttcgtcgcta tatggggaaa gtggagtact aggaggatta
     3241 aaatgaaggt attaggctta ggtgataacg tcgtcgataa atatgaaaat ctgggaatta
     3301 tgtatcctgg aggaaattca ttgaattttg cagtttttgc aaaaaaactg ggacatgaaa
     3361 gtagctttat gggagttttt ggctcagatg aggaggcagc tcatgtttta tcagcaataa
     3421 aaaaaatagg actggacaac agtcactctc gctttgaaaa aggcgagaat ggctgtgcca
     3481 gagtgaagat tgatgagggc gaccggattt tccttggcag caattcggga ggtgttactc
     3541 gtgaataccc gattcagctt actgaagaag acagagaata tttaaaccag tttgaattga
     3601 ttcatatggg attatatagc catgtcaatc atctattaga agagttacaa aatgtttctg
     3661 gaaagatttc ttatgatttt tctgacgact ttacggaaga agaggttcag cgggcaattg
     3721 ataaagttga ttttggcttc ttttctatag aagacttttc cgatgaagaa gtaaagcagt
     3781 ttttgatgaa gcatttttgt gagcgaaatg atgtattgat tgcaacccga ggagaaaaaa
     3841 cggctattgg ttatgatgga gcaaagtttt atgaagtgaa ccccgtgcta gaagaaccag
     3901 ttgatacaat ggcagcggga gattctttct taacagcttt cctactttat tttctcgaag
     3961 gggacgatat agtcaccgcg atgaaaaagg ggaatgaatt tgctgggaag tcttgtctgg
     4021 ttgacggttc gttcggcttt ggtattcatt acgattgagc ttgttttaaa ttcctgcatt
     4081 tgataaccta aagaaagaaa tcccggtaaa gtggaggtta aaaaaatgct ggatacaaat
     4141 gcacaattac cattgtactt acaattaaaa gaaatattaa aaaataaaat caatacaggg
     4201 gagtatcccg agaatacgaa aattcccgct gaatcagagc tgatagaaag ttatgaggtc
     4261 agccgaatca cagtgagaaa agcagtagaa gaactggtag aagaagggta cctacttaaa
     4321 aaacaaggga aaggaacttt tgtcaaccgg cataaagttt ttcgtaaaat tgaatatgtt
     4381 atgggtttca gtgagagttg taaatctaat ggttttacac cgactagcat tgttttggaa
     4441 cgcaagatca ttccagcaac tgaagggatg gcaaaaaaac tcgatatcgc tcaaggtgat
     4501 gatgttttat atatccagag aaagcgaatg gcggataaca tccccattat gttagaaaac
     4561 aattattttt cttatgaaag atatagtttc ttaatggagg aagagctgac tgattctttg
     4621 tatgaagtac ttaaagtcca aggtacctta gccatcaatc ctggggaaac tgcacttgaa
     4681 attgtcacag ctgatgacca aattgccaaa ataatggaag tagctgttgg gacgcccttt
     4741 ttctttatgg atactttaat caacgatcaa aacaacatac caattcatgt aggtaatcaa
     4801 tattacctgg gagaagtata tcgtttttac ttatgatttc ctgttttgat ttcaaggagt
     4861 agattttatt tatgaagcaa atattgcggt caaaaatgat ttttgatgga gagcagccac
     4921 ctttcaaagg ttatattgta atcaatgaag ggatcattga aaaggtggaa aacaactgga
     4981 attacagtga tctattaaat gaaaacacgg aattaattgc ttatgatgat caattcgtta
     5041 tgccagcaat tcatgataat catgtatttt tcagtggtta tttggctatg aatgctggca
     5101 tagatttaag cgatacatct tcagctaacg aggctgtaga gcgtattaaa gctgtaatga
     5161 accataaaaa agcagatgaa ccaatttatg ctcatggctg ggatgctgcc ctgtggaaaa
     5221 ctgacccatc agaagagcta ttgaatgaaa taaactactt tggtccaatc acagcgatcg
     5281 acaaaaatcg cagccattgt tggatgaatg ctattgctaa gcagacttat ggttttacag
     5341 aaaaagaaac cagtgctgaa agtcgagtat tattaattaa ggaaatgcta gcaaacaaaa
     5401 agcttttagc agaggtttac gctgaatttg aagcattact gctttcaaaa ggtgttatat
     5461 caatgaaaga aatcgttttt gatgacgcag aatttctttc taatatacct cagaggaaaa
     5521 tacttagtaa cctatatgtg caggcagtcc aagaacctct acagcatgaa cttctatatg
     5581 agtataagga aaaagttttc cctgaaaagg tccgctttgg tggagtgaag atcatggtag
     5641 atggtgttgt tgcggataga agtggagata tttacggtgt ttatcagtca ggagtagctt
     5701 cacccgagat tgactatgag gcaattaaag cagaagttgc tgcattcaat gaagtagaga
     5761 ttccttgctg tttgacaacg gaaggagata aagcgggtta taaagctgcg tcaattcttg
     5821 tcgaatttgg aaagcggtta cctgaaggcg tttttaattc gattagtgat ttggaaatgg
     5881 ttactcaaaa gacagcagaa aaaatgaacg aaggacaagt agtagcagag gtatatccac
     5941 aaattctcgg cttgaatcaa acgttagaag aggcttacat gtcaactgtt ataaaaaat
//
LOCUS       DACEKQ010000128         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEKQ010000128 DACEKQ010000000
VERSION     DACEKQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.136"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW671_002575"
     CDS             641..1375
                     /locus_tag="IW671_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0899532.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW671_002576"
     CDS             1391..2560
                     /locus_tag="IW671_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899533.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW671_002577"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW671_002577"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0899534.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW671_002578"
     CDS             3650..4576
                     /locus_tag="IW671_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0899535.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW671_002579"
     CDS             4595..5845
                     /locus_tag="IW671_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0899536.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          956 c         1203 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgcagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACEKQ010000129         5678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEKQ010000129 DACEKQ010000000
VERSION     DACEKQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.137"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IW671_002580"
     tmRNA           complement(240..606)
                     /gene="ssrA"
                     /locus_tag="IW671_002580"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(777..1550)
                     /locus_tag="IW671_002581"
     CDS             complement(777..1550)
                     /locus_tag="IW671_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0899537.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1694..2044
                     /locus_tag="IW671_002582"
     CDS             1694..2044
                     /locus_tag="IW671_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0899538.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2025..2741
                     /locus_tag="IW671_002583"
     CDS             2025..2741
                     /locus_tag="IW671_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899539.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2741..4189
                     /locus_tag="IW671_002584"
     CDS             2741..4189
                     /locus_tag="IW671_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0899540.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4259..4768)
                     /locus_tag="IW671_002585"
     CDS             complement(4259..4768)
                     /locus_tag="IW671_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899541.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4758..5483)
                     /locus_tag="IW671_002586"
     CDS             complement(4758..5483)
                     /locus_tag="IW671_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899542.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1936 a          984 c         1120 g         1638 t
ORIGIN      
        1 gtttttcctt ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt
       61 ttgtaaagta ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt
      121 tttgaatgct attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa
      181 atgctcaaac ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac
      241 aatggagacg gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt
      301 catagtcaac tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc
      361 gctagtctga tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa
      421 gttaggaccc ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta
      481 ggcagctaaa gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt
      541 tacgtagacg taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac
      601 gtccccgtta gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat
      661 tgggataaat tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt
      721 tcttaagaaa aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa
      781 atcagatcaa aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata
      841 aaggtagaaa gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa
      901 gcttccaaca aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc
      961 agctgaagtt ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact
     1021 gtatcgatcg agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag
     1081 tattcgtcac ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct
     1141 aaaggatcaa tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg
     1201 ctatgaccgg tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac
     1261 tgtacatgat catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct
     1321 tcgccgtcta ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct
     1381 tccatgatag agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg
     1441 ttttctttta atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct
     1501 agtaatgttc catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact
     1561 ccttttttat tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg
     1621 aagaattcct gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt
     1681 taggagggaa agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct
     1741 ttttcggatt ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc
     1801 cgcaaaaaca gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct
     1861 tatgggctta ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta
     1921 ttttctgatt gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga
     1981 aaagaaatta cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga
     2041 ttgaagataa tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg
     2101 aagcaacttt caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac
     2161 attgggatat gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa
     2221 gagagatccg caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg
     2281 aaagcgatca ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca
     2341 gtcctctgac gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag
     2401 caagagaaga agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga
     2461 tgaatacgaa aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta
     2521 aagagttcga tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc
     2581 agttgcttga attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc
     2641 atatcaagaa attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt
     2701 ggggagtcgg ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca
     2761 actcttagct ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac
     2821 acaactgaca aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga
     2881 atcagttgaa aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca
     2941 ggcgtttcaa aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt
     3001 tatatttatc gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt
     3061 taccttaaca gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc
     3121 gaataaaaat attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt
     3181 gaatcatgaa ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag
     3241 cgttcgtcca attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt
     3301 tattgccagc tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc
     3361 gacgaaagaa gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga
     3421 atttgatgag ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc
     3481 agaaattgaa gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat
     3541 gagaacacct cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc
     3601 tgaaaatcag cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg
     3661 tttggtgaat gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat
     3721 taaaaaattc aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc
     3781 agaagcagca ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga
     3841 ttatgaccga tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga
     3901 aaacggccaa atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga
     3961 agatacggga attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa
     4021 agtagatcct tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt
     4081 gcagcaactt gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg
     4141 aacaaaattc gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg
     4201 tataaaagat catgaatcga tcttctatac cgcttgtttt tactataaaa tgattgattt
     4261 agcccgcggg ctctaaacga tcaatataag gactttgagc aatagtagca tcatgagtaa
     4321 agagtaagat accttgattt tcttcatgag ctaaataaga aagcaagtgg agtagctctt
     4381 ggatctccaa tacactaagt tcatcaaatg tatcttcaat aacaatattg tttcgcttgg
     4441 aaagcaaggc atgaagtaat tgcagcttta ctttttcaaa agacgtcaac tcagctgccg
     4501 cacaattaag caaggaagga gagatatcaa gtttttcttg ataggccaaa cgatcttgtt
     4561 tgatttcttt ttccggtaca tccaacagga catggtcttt aagattcaga taaggaaaca
     4621 tcggccaatt tttttggatg atcgcaaaat tatccagttt tgcaacaaga acctcttttt
     4681 tcaagtgctc caatatttta tgctgttttt cgagaggaca caaaataacg gtcaagtgtt
     4741 cagcatgaat caaatattca ggtttcatta cgtccaggcc tcctactttg caaatgatgg
     4801 ataaaaaaag tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga
     4861 gtttgggcaa atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta
     4921 aaaaaagtct gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg
     4981 atatctggca gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca
     5041 taaactgact ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga
     5101 aagaacagct gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc
     5161 caattgatca attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg
     5221 aaggcaaata gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct
     5281 aaagccggaa gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact
     5341 tgattggcta aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca
     5401 gaaaagcttg aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca
     5461 gtagcatagc ggattatttt catacttttc acctctataa aaagtataga aaatgaatat
     5521 gaataaagta tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa
     5581 aaagcaagag gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac
     5641 actgtttatt cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DACEKQ010000130         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEKQ010000130 DACEKQ010000000
VERSION     DACEKQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.138"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW671_002587"
     CDS             complement(72..542)
                     /locus_tag="IW671_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0899543.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW671_002588"
     CDS             872..2512
                     /locus_tag="IW671_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0899544.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW671_002589"
     CDS             2666..3988
                     /locus_tag="IW671_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0899545.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IW671_002590"
     CDS             complement(4056..4490)
                     /locus_tag="IW671_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0899546.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="IW671_002591"
     CDS             complement(4555..5391)
                     /locus_tag="IW671_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0899547.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1128 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgtt
//
LOCUS       DACEKQ010000131         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEKQ010000131 DACEKQ010000000
VERSION     DACEKQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.139"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..1107
                     /locus_tag="IW671_002592"
     CDS             133..1107
                     /locus_tag="IW671_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899548.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IW671_002593"
     CDS             1089..1550
                     /locus_tag="IW671_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0899549.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IW671_002594"
     CDS             1600..2064
                     /locus_tag="IW671_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0899550.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IW671_002595"
     CDS             2078..2827
                     /locus_tag="IW671_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0899551.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IW671_002596"
     CDS             2827..3657
                     /locus_tag="IW671_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0899552.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IW671_002597"
     CDS             3657..4304
                     /locus_tag="IW671_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0899553.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IW671_002598"
     CDS             complement(4402..5178)
                     /locus_tag="IW671_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0899554.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IW671_002599"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IW671_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACEKQ010000132         5199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEKQ010000132 DACEKQ010000000
VERSION     DACEKQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.140"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1868
                     /locus_tag="IW671_002600"
     CDS             <1..1868
                     /locus_tag="IW671_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0899555.1"
                     /translation="TPPEKVNITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTK
                     YNKADYGDVEFTLGNITDQVLPTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKIT
                     SAVDENGEITFADQPAYVNSKGNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGF
                     LKDIHLYPKNIVSKLSFELTKFGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVS
                     DDQGKLTATDLTLGKYYFVEVPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITN
                     DGVTSDLKASYVNYKAPVIDKTVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGI
                     TVNGVKSETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSEN
                     GFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQ
                     EEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDK
                     DGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKV
                     DKDSYKEENRITIKNNQKASVPMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            1881..2633
                     /locus_tag="IW671_002601"
     CDS             1881..2633
                     /locus_tag="IW671_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0899556.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2649..3404
                     /locus_tag="IW671_002602"
     CDS             2649..3404
                     /locus_tag="IW671_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899557.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3417..3677
                     /locus_tag="IW671_002603"
     CDS             3417..3677
                     /locus_tag="IW671_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899558.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            3674..>5199
                     /locus_tag="IW671_002604"
     CDS             3674..>5199
                     /locus_tag="IW671_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0899559.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     "
BASE COUNT         1893 a          868 c         1047 g         1391 t
ORIGIN      
        1 aaacgccacc agagaaggtc aatatcacgg ttcataaatt aatgtacgat caaagtacgc
       61 aattgaatgt tgatattgac gggatcaaaa atgatggtta tacgcacgat cagtatccta
      121 caggtgtaac gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata
      181 ttacggatca agtattaccg actgaagatt ctgatctaac caacgcgaaa gtggacgaaa
      241 tcgtgaaaga cgtagaagac aagggatcta acagtgagta cgtcaagaat gcaaccaata
      301 aaattacttc agctgtagat gaaaatggtg agattacttt tgcggatcaa ccagcgtacg
      361 tgaactcaaa aggaaatgtg tatgttattt acgagagtaa gtccgcagca ggattagtaa
      421 ctcaaaaagc aaaaccaatg gtggtcattg ctccaatgac tgacaacaca ggttcaggtt
      481 ttttaaaaga cattcactta tatcctaaaa atattgtttc taagttgagt tttgaattaa
      541 ccaaattcgg tgatgatggt acagagcaaa gcaaacaaac ccctcttaaa ggcgctaaat
      601 tcgaattgta taaaggagaa cctggaaaag gaagcaaact aggcgatctt gtttcggatg
      661 atcaaggaaa gctgactgct actgatttga cgttaggaaa atactatttt gtggaagttc
      721 cttcagaagt cgtagtggga tcagataaag aaccaacagc cgatcaatat ttattaggcg
      781 cagacgctcg caacgatgcg cacaataaat taacctttga aattacgaat gatggcgtga
      841 caagtgactt aaaagcttca tacgtaaact ataaagcacc agtgatcgat aaaacagtaa
      901 caaatggaac aggacaagaa cattctttcc aagttggtga tgccgttaat tatcaaggaa
      961 cgattcatat cccaacagac attgcaggcg gagcagacgg tatcacagta aacggcgtga
     1021 aaagcgaaac ttctccttac agtgtattta aatgggggga tacagcagga caaggacttt
     1081 cttatgtcgc tgctaaagca aatatcaaag taacaaataa agatggatca gtggtattaa
     1141 aagaaaatac agattataag atccaaaatt cagaaaatgg ctttgtgatc gattttatcg
     1201 taaataatgg ccaagtaagt gatactgtag caagtttaca cggacaagat cttcaaatga
     1261 cttataacat gtatgtaaat gacagcgcag ctgttgccaa tccattaaca aatagcgtgg
     1321 atttcgttta taacaacaat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg
     1381 acgttgtgac ttatggcgcg aaattcttaa aagtcgatag cggactattt ggaacaggaa
     1441 ttaaggcaac tcctcttgaa ggcgcagaat ttgcagctaa gaatgcagaa ggtaagtact
     1501 acggtggact tactgataca gataaagatg gtgtcaaaga agcagtatgg gtagacgatg
     1561 tagcaaatgc agcaatccta aaatcagata aagagggtca ttttgaaatc actggtttaa
     1621 ctgaaggaga ttactcactg gaagaaacaa aagctcctga aaactatcaa aaattaacaa
     1681 aagagatctc tttcaaagta gacaaagatt catacaaaga agaaaaccga atcacgatca
     1741 aaaataatca aaaagcttcc gtaccaatga ctggtagcaa cggtttccaa tcttacgtat
     1801 tgatctcttg cttgttgctt ggggcaggcg caatttctgc agttgtttac tttaagaaga
     1861 aagcctagaa ggatcagcta atgattatca gacaccctaa gaaaaagcga atcatgggta
     1921 aatggatcat tgctttctgg ttactttcag ctgttggtgt cttacttttg atgcctgctg
     1981 aagcgagcgt ggcgaaatac caacaaaatc agcagattgc cgcaatcgat cgaacaggaa
     2041 cggcagcaga aacggattct tctttggatg tttctaagat tgagttaggc gatcctgttg
     2101 gtattcttac aattccaagt atttctttaa aactaccaat ttatgacgga acttccgaca
     2161 aaattttaga gaatggtgta gggattacgg aaggaactgg cgatattaca ggaggaaacg
     2221 gtaaaaatcc tttaatcgca gggcacagtg gactttataa agataatctt ttcgatgatt
     2281 tgccttcagt taaaaaggga gaaaagtttt atatcaaagt tgatggtgag cagcacgctt
     2341 atcaaatcga tcggattgag gaagtgcaaa aagacgaact acaaagaaac tttgtcactt
     2401 acttagaacc aaatcccaat gaagatcgag taacgctaat gacatgtacg cctaagggga
     2461 tcaacacaca ccgtttccta gtgtatggga agcgagtaac ctttacaaaa tcagaattaa
     2521 aagatgaaga aaataagaaa caaaaattat cttggaaatg gcttttagga agtactgttt
     2581 ttctttcagt catgatcatt ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg
     2641 taaccaacat gagaaataga aaatggctat ttgtttgtgc agccgctctt ttttctttca
     2701 ctgcctacac acagttgacg tttgcagaca gtcaagaaag cacggatagc acagaacaaa
     2761 caatccaaac gtcagatagc agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa
     2821 cagtggattc ttcagaagaa ccgaaaattg ctgaagacga tcaaatagtc aaacagacca
     2881 ttcacgttca aaagattatt tcttcagaac aattagacaa ccaggggcat gaaaaacaag
     2941 aaggagtaaa tggcgcgaag ttttctgttt atgatgtatc ggacatttta caaaagatgg
     3001 atgttaagga tctcactact gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac
     3061 tatccagtga tcaattgaag ctagtttcta atggagaaac aaagacaatt gatcaacaaa
     3121 caggtgtctt tgaattctcg gtagaagtac aggcaaatca gaaacaagct tattatattg
     3181 tcaatgaatc aagtcctgaa aacatttcta atagcgagga tattttgcta ttaacacctg
     3241 tttctgaaaa aaacggtgaa ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt
     3301 cgcaacctaa agaagaagta aaaaaaatcg tttctactgg tgtaaagaaa aacttctttg
     3361 agaattgttg ggattttgtt actcacttgt tttctagaga ttaaggaaga aataagatga
     3421 aactgatcaa attattatta ttcaatcttt cttgtttaac cgcagtcttt ttgtactgcg
     3481 gtttattatt ttctcaatta tctattttgc caagctacca actataccta atgaggattt
     3541 taaacatacc agctgcttcc atgccgctag atattatgga aaagctcatt aggaatccat
     3601 tatttactac gatcaatttg atcgttattg tcggaatttc actagtcatt tataaaaaag
     3661 aggggatgaa gggatgaatt ttaaacactc gtggaccaga cggttaaaat atgtttttcc
     3721 cataatgatg atgttaggta cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa
     3781 agttattgtc gatccgtcaa atccaatcca gaacgtaaag aataatttta ttattccaaa
     3841 agatgttcct aatgcaaaaa caaatattac tgtaaacgga gcgtccaaag tttacaaata
     3901 tcatgttgat gccaacaaag gtggttttac agacgactac gtaggattag tggagggaaa
     3961 caaaaatata cgctatcctt ctttccaaaa ggaatacggt gaaacaaacc ttgttttaga
     4021 aggcgtaaca accaacacca aagtcaatat tgactatgga aaaatcggta cgtataacgg
     4081 gaaaaaagtc aatatcaagc tagttctatc taatattcac ttatactcgg atacgttgcc
     4141 gtggaatcta ctagataaca actatacaaa aactcatttt cgagacgatg gctataaaaa
     4201 tacgaatggt gcaatgagta aatctaaaaa aagaacagta ctttggatct cagacaatct
     4261 tttctcagga attgtttacc attcaacaca aatgaatgtg cagctagtag ccacatatga
     4321 ggacggtagc cccgttcaat tttcaggaga tacatttatc agttttaact ctttgaaccc
     4381 tgcaggagga aaaagcacgg atcttaaagg agaatatgcc cactatgaca aaatgaatac
     4441 gacagattgg tatgtcagac aagatgcggt tctttcagaa tttaaatctt tctacaacaa
     4501 tctgaacgtg gtaggtggcc acccaggagg aagctctaag ctgacacaag cggataatga
     4561 tttcaataat ttgcatgata aattaggaga tccgaagttt ggtcaaggta ctgtatcctt
     4621 taaaatatct gaagcaaatc cgacgtttgt gattggttct tctaatgttc aaacctggtt
     4681 tactttatct tctgcgacaa tctttagtgt tgttcctgat caacctgaaa aaacaggggt
     4741 tgataaaaat ggaaataacg tgaacgataa aatgttgcaa gtaggagaca cgattcaata
     4801 tcgaatcaaa cagaaggtga atcgattagg agtggattta ctagcggttt acgatcgatt
     4861 tgagttaatc gataatttac caaaagaagt agattacgtc gatgcacacg ttgaaagtgg
     4921 tacaaataaa aaattcgatg tatctggtga agtgacctat gacaaaacaa aacaccaggt
     4981 aaaatatgca gcgaaagctg atacattaaa aaatcgaatg aagtataacg gcgaaactta
     5041 cgaattagtg atcaatgtaa aagtcaatga attggccaat caaaatagtg tagccaaaaa
     5101 tcagggaacg tcgatcatta ataaagtaga aaaggataca aatatcatta ccgtttattt
     5161 ccctaaaatt ccagtgaaag aagtacaaca aaatggaaa
//
LOCUS       DACEKQ010000133         5055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEKQ010000133 DACEKQ010000000
VERSION     DACEKQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.141"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(169..1977)
                     /gene="pepF"
                     /locus_tag="IW671_002605"
     CDS             complement(169..1977)
                     /gene="pepF"
                     /locus_tag="IW671_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0899560.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2153..3151)
                     /locus_tag="IW671_002606"
     CDS             complement(2153..3151)
                     /locus_tag="IW671_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0899561.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3377..4030)
                     /locus_tag="IW671_002607"
     CDS             complement(3377..4030)
                     /locus_tag="IW671_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0899562.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4362..4760)
                     /gene="spxA"
                     /locus_tag="IW671_002608"
     CDS             complement(4362..4760)
                     /gene="spxA"
                     /locus_tag="IW671_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0899563.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1506 a         1044 c          775 g         1730 t
ORIGIN      
        1 ctaaaggatt tcagggtgtc ttttctatat tttcttatac aaaaaagatg ttggtgaagt
       61 tcaccaacat caaaaagtat agagccttaa aaaaatacgt cgactccctt acaagagagc
      121 cgacgtattt ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc
      181 gatcaattct tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata
      241 tgcagcctgt gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt
      301 acctgctttt aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt
      361 cttcgccagt gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata
      421 ataaaaatgc ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg
      481 accataatat tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg
      541 cacacctttt tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg
      601 gaaaactgtt cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg
      661 gtctttttct gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat
      721 ttctgctaaa aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata
      781 actatgaaca ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg
      841 ccaattcaat aaaatatatg gatttgtgtc atagcttccc gaagaataag caccgcttcg
      901 tttcccttta ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc
      961 catgtattct tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta
     1021 cgtaaaggta attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt
     1081 ttccacttct aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt
     1141 gtttaccacg tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga
     1201 cgcttctctg gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg
     1261 tgtgcctaaa gtagaagcaa atgtatttct aaattgttcg taaacactgt acaacccctt
     1321 aaatgctgct tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg
     1381 agataattgg actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt
     1441 attcaaaaca gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag
     1501 agattcctgc tcagcagata agacatgggc tcgattatct actatttgct ggatataatg
     1561 gcgatagact tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa
     1621 ttgcaatatt tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct
     1681 tgctcttgca taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg
     1741 cgcatataca taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt
     1801 taaaaattga gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc
     1861 ttctgataat tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa
     1921 gtcccaagtt aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa
     1981 atccactcct tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac
     2041 atgtcacagc atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata
     2101 actaaatctt gttttacata gaaaaccgat tcctagccta tttttatact tactattttt
     2161 tcaaagaaga caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa
     2221 tcataggaaa aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat
     2281 caggaacttt ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt
     2341 cttgtaaata aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat
     2401 gtttatgacg caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa
     2461 gttggcgtgt cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt
     2521 tcattttaat ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc
     2581 agctactgca aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa
     2641 gcttttgttt cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc
     2701 agcaactttt ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc
     2761 accatacttg gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag
     2821 acagtctaga acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc
     2881 gcaacctcgg caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca
     2941 tgaagcattc ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt
     3001 tatctgattt ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc
     3061 tttttcgaca gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc
     3121 acttattata ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta
     3181 agaatttatt tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga
     3241 acagctaaac aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg
     3301 caagcttttg aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc
     3361 ttactataat gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca
     3421 tcagacattg tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact
     3481 ccaaaattcg agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt
     3541 gaacgactag gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt
     3601 cgacatctgt tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc
     3661 tttcctgttc ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct
     3721 gctgacgaag aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa
     3781 tttcttcatc cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa
     3841 cttggaaagt gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa
     3901 tactataaaa gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc
     3961 cccgttcctc taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt
     4021 ccatttccat tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg
     4081 aaatcagctt aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc
     4141 ggatttttca acctttcaag ctatcttatt taactataac atttttggca gacgaatact
     4201 atatagccga cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta
     4261 ctaccttttt atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa
     4321 ctatctttgt aatagtcttt tccaagtttc gtattatcct tctataatcc agccatcaat
     4381 tgtgcttgac gtaattctaa ttgtcgtaca tcgcgcggta ggaaccgacg aatttcatct
     4441 tcattaaaac caacttgcag acgtttatca tcaatcatga ttggacggcg aagcaatcca
     4501 ggattgtttt gcacaagttc caataattct tgtaacggca gatcatctaa atccatgttc
     4561 agtttttgga atactttgga acgtgtcgag atgatttctt ctgttccgtc ttctgtcatt
     4621 tgtaaaatcg cttttaattc agaactgttc aatggttctg aaaaaatatt acgttcaaca
     4681 aatggaatct gatgttcttg caaccacgca cgagcctttc tgcaggaagt acaacttggg
     4741 gaagtatata atgttaacaa cgtgcaccac tccttttcgt gataagaatc acttttttgt
     4801 aacagaagat cataataatc tcccatctgt ttctattata cataatcaac ttttaaatag
     4861 ctaccttttt ttcataattt ttttaaaaaa atgaaagtta tctaaggtta atatacagta
     4921 aaaccagcat tctcgctttt tttatcattt aaaaattaga gttttcaaat ctaattcaaa
     4981 ttttctaaac atatgaaaca gcgtataaaa ctgtactatt attttatctc aaaagctaaa
     5041 tgaaaccgat ttctt
//
LOCUS       DACEKQ010000134         5030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEKQ010000134 DACEKQ010000000
VERSION     DACEKQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.142"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(177..680)
                     /locus_tag="IW671_002609"
     CDS             complement(177..680)
                     /locus_tag="IW671_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0899564.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(803..1567)
                     /locus_tag="IW671_002610"
     CDS             complement(803..1567)
                     /locus_tag="IW671_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0899565.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1675..1950
                     /locus_tag="IW671_002611"
     CDS             1675..1950
                     /locus_tag="IW671_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0899566.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(2151..2498)
                     /locus_tag="IW671_002612"
     CDS             complement(2151..2498)
                     /locus_tag="IW671_002612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899567.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            2666..3595
                     /gene="yidC"
                     /locus_tag="IW671_002613"
     CDS             2666..3595
                     /gene="yidC"
                     /locus_tag="IW671_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0899568.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            3698..5030
                     /locus_tag="IW671_002614"
                     /pseudo
     CDS             3698..5030
                     /locus_tag="IW671_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1592 a          962 c          943 g         1533 t
ORIGIN      
        1 aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctt gcatctgcag
       61 taataagccg aaacttcaca aaaatttgag aagtaatttt cgtaaattcc ttcttatttc
      121 ttgaagctga ccacttctgt cacaacctca atatggtcta ttattggttt tacctgttaa
      181 atcgaggatt ctttcccaaa gtaacgttgc catctcttac gcatacttgc agacatgggc
      241 tgagccgctt ctttgactgc gcagtatttg tccacgaatg tcacaatata tccttcttta
      301 tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca aaatgatatc tcgctcaaga
      361 tccgacaaat cggtgatttt ttctgcattc tttacagcaa ttcttggatg gatatacgca
      421 tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt
      481 ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag
      541 taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct
      601 aattttttca ctgcttctgt ttctaaaaga tcttgtacat aagacatata ttcttcgtct
      661 tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct
      721 gaaattgatc taagtatacc accagtgttc ggattattct agtcgctcta gataaaatag
      781 ctaaagctta atagttcctt aatcataaag aaaaatgata aagaacgatc cctgctgcga
      841 ctccgacatt caatgattcc gcatttcctt tcattgggat atatacgttc tgatcagtca
      901 aagacaagat ttcctgactg actccctgac cttcgtttcc catgattata gcgaaatcct
      961 ctgttttttc tactttgtct aatgcatgtg cttcttcatt taattctgta ccgtacaccg
     1021 taaacccttt gcctttcatt acagggatta catctgccaa atctttttgt aacacaggca
     1081 aatgataatt gcttccctgc atgcttcgca gcacctttgt actataaata tctgccgttc
     1141 ctttacctaa taaaacacca gccataccaa acgcatctgc cgtacggatc atcgttccca
     1201 catttccagg atcctgtaca ttatctagta aaagccagcc tcctgaaaaa tctggcgtat
     1261 cttcagatgt taacgtcatc actgctaaga tcccttgcgg tgtaggtaga tctgacatag
     1321 cggtcatgac ctcatctgaa actagaaaag tagaaatctc tttgtttaca gccagccagt
     1381 ctccccattc tttcatacct ctctgtgtca caaaaagttc ttttatttct gcatcagacg
     1441 acgcagcttc ttcgatcaga tggaaccctt ccaaaagata ttctgccttt tcttctctgt
     1501 atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct
     1561 ctttcacatt ccttcacctc acctgattat atcatggaat aggtgcttga gcttgtttca
     1621 tcctaaaaaa atcggtatca ttgatttata aaacaggaaa gaaaggtgac gaacatgcgc
     1681 aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg gatttcgcta tatgaccaag
     1741 atggttgctg atcagttagg cgtgaccgga acggtaaaaa acgaagacga cggctcagtc
     1801 acaatcgaag caatcggaaa tgacgacatc atccaaaaat ttatcgaaga agtcaagaag
     1861 tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagatcc gatgattgag
     1921 gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aggtcgctta gttttgagta
     1981 ttaaggaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttaataccg
     2041 agaaactgac ttttgaacac cgtcgataag gttgtttttg aagcgctttg cctcgagtat
     2101 taaggagaaa cattttcaaa atagcttctc atattttgaa atgtaatgac ttaataccga
     2161 gaggcagctg ataaaacacc gtcaataagg ttgtgaaaaa gctggtctgc atcaagcatt
     2221 aaggactggt tgtgaaaaag tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
     2281 gctttccaca tttttgcata aatttgtctt aataccaaga aactgacttt tgaacaccgt
     2341 cgataaggtt gtttttgagg cgctttgccc caagtattaa agacaggtag taaaaaagct
     2401 gatctatcac ttgcagtgag ttcatttgac tcctctattt cgggcagaat aaaaaaagtt
     2461 tttagttttt taaataagaa agaaacactt ggaatcactc ttttctttcc aagtatttct
     2521 ttcttgattt tttgtctttt ttcaaaattt ttcgctacga aaaagaaaga atatttatat
     2581 gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta agtttgtgta
     2641 aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat tgggatccgg
     2701 acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg atggattcaa acggaaaccc
     2761 tgacacttca ggaatcgttt atcgagtact cgtccatcct atgggccaag cgattacata
     2821 tctagttgag aatttcaatt ggtcttacgg ctgggcagtt attgcaatga ctgtgatcgt
     2881 ccgtatcatc atcttgccac taggtatcag tcaatcaaaa aaaacaatga tccaatctga
     2941 aaaaatgcaa gcgttaaaac cacaagttga agcagcacaa caaaaactaa aagctgcaac
     3001 aacacgtgaa gaacaaatgg cagcacaagc agaaatgcaa caagtatatc gcgaaaacgg
     3061 attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc cgatcttctc
     3121 tgccttgtat tttacggctc gctacacaga aggtatccgt gaatcatctt tttacggcat
     3181 cgatctaggc tcaccaagta tggtgcttgt tgccattgca ggggtcgctt atctgcttca
     3241 aggatatatt tcaacaatcg gtattcctga agaacagaaa aagacgatgc gtacgatgct
     3301 gatcgtctct cctgctatga tcgttttcat gtcgatcagt gcgccagccg gtgttacact
     3361 ttactgggta gtgggtggtg tgttcagttg tctgcaaaca ttcatcacaa acgttatgat
     3421 gaaaccacgt ttgaaagcac aagtggcaga agagttgaaa caaaatccgc caaaacaagt
     3481 cgtcactcca agaaaagacg tgacagaatc ggcaaagcca gctgacaata aacaaccaaa
     3541 aaacagtaca aacaacaaag gccgcaacgc aggaaagcag caaaagcgga aataaaaata
     3601 tatgacaaaa aactgtagac aaataaaaaa atttctttgt tctacagttt ttttgttaca
     3661 atcaaagaaa acgtcgcttt tcgaggagga atgtataatg atgtccaaat aatcaatgga
     3721 tagtcgaatg gaagaagcca aaatttcgct agatgcactt gttcctaaaa atcatttggt
     3781 ccataaaatt gacaaaatat tgttatttga ttttatttat ccaattgttg aatcaactta
     3841 ctcgaccatt ggtcgtccta gcgttgatcc tgtcatctta gtaaaattag tattcattca
     3901 atatttattt aggattcgtt cgatgcgtca gtccattaaa gaagctgaaa caaacctagc
     3961 ttatcgttgg tttcttgggc ttagtttaat cgataaaatt cctcattttt caacgtttgg
     4021 taaaaactat gtacggcatt tcagagaaac cgattgtttg aacagatatt tatcattatc
     4081 cttgaacaag cagttgaagc tagatttatt catgaagaaa ttttgtatat ggattcaacc
     4141 tatattaaag cgaatgcgaa aaaacatatt tttacaagag aaatggtaca taaataagcc
     4201 attttatacc aaaaacaatt gaaagaaaaa attaatgaag caagaattgc agaaggcaaa
     4261 cgtccttttg gttcaatcat tcatcaggag gtggtagaag ggaaaattag tgtcgcagat
     4321 ccagaaagtg gttattatgt aaaaacagaa caagaaaaac agtttgctta ttctgctcat
     4381 accgtttgtg atgaaaatgg ttttgtatta gatgtcatga ttacgcctgg aaatctacat
     4441 gacagccgaa tgctcgtgcc acaaatcgaa cgtgttaaat catgttgtgg tgtagctgct
     4501 gatgctgcgt acaaaacacc ttggaacgcc aagtatttaa tcgatcgaaa actacgaccc
     4561 atttttcctt atactcgacc caaaagaagc aaagaacgtt ttaaaaagaa agatttttat
     4621 tatgatccct attataactg gtacctatgc ccaaatgatc aaacacttca gtttcgcaca
     4681 actactcgag atggttataa aaaatatgta tctaaatctt ttatttgtga aagttgtcta
     4741 ttattgaaaa attatacaga aagccagaaa caccagaaag aaatacatcg acatatttag
     4801 gaagactact tagatgaagc agaacattta cgccatacgg tgtacaatcg cgaaaaatat
     4861 aaaaaaaaga aaagaaacga ttgaacgcgt ttttacagac gcaaaggaaa agcatggaat
     4921 gcgctacacc aaatatcgag gcttggaaaa aaccaggtgc atacgatgct tgtttttgcc
     4981 gcaatgaatc tgcaaaaatt agccatttgg cagtgggggc atatgtcttt
//
LOCUS       DACEKQ010000135         4820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEKQ010000135 DACEKQ010000000
VERSION     DACEKQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.143"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW671_002615"
     CDS             complement(207..1169)
                     /locus_tag="IW671_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0899569.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW671_002616"
     CDS             complement(1171..2610)
                     /locus_tag="IW671_002616"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0899570.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW671_002617"
     CDS             2795..4741
                     /locus_tag="IW671_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0899571.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1541 a          823 c          792 g         1664 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat
//
LOCUS       DACEKQ010000136         4807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEKQ010000136 DACEKQ010000000
VERSION     DACEKQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.144"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW671_002618"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW671_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0899572.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IW671_002619"
     CDS             complement(3250..3768)
                     /locus_tag="IW671_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0899573.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACEKQ010000137         4775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEKQ010000137 DACEKQ010000000
VERSION     DACEKQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.145"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(542..1582)
                     /locus_tag="IW671_002620"
     CDS             complement(542..1582)
                     /locus_tag="IW671_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0899574.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            3394..3612
                     /locus_tag="IW671_002621"
                     /pseudo
     CDS             3394..3612
                     /locus_tag="IW671_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3872..4126
                     /locus_tag="IW671_002622"
     CDS             3872..4126
                     /locus_tag="IW671_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0899575.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLIILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            4127..4444
                     /locus_tag="IW671_002623"
     CDS             4127..4444
                     /locus_tag="IW671_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0899576.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT         1664 a          700 c          734 g         1677 t
ORIGIN      
        1 ggtaaacgca ttaagggatt aaaggttgtc ttaatgccac ctgtggacgt cgacgaaggt
       61 taccagcctt ccgagtctca cagggccttt cttttttttg tattttcaat tgtccaaatt
      121 tatcgtccaa aagtgcagaa cgaaaattaa ttcatcacaa gcgtagcaga caattaaatt
      181 taaaacaact aaatttatac aaaaaaatgt aagcgaataa tattcgtatt gcctgaaaag
      241 aagtaatatc aaggttttta gcttttttaa aaaatgaaat tacctctctt cgcgtattat
      301 tataactaaa aaacagcaac atgcaaacgt ttgttctctt ttttgtgttg gaaattaatt
      361 attaactatt aacacttatg acgtaaaaag ctacgtttgt gatttttatc ataatatttt
      421 aactcataaa ttctattaaa atagctattt ctttgtaaca tttgtaatat aattagaata
      481 ataaaaaatt tttaatttta atgctactcc atgaagcaac aatcaatttg tcagtgaaat
      541 ttcactctaa ccagtctttc gtaatagttt ttgggaagct ttcagaattc aaacgttttt
      601 cacttgatac tttagcagct attggttttt cgataaacca attttttaca tagacaaaaa
      661 tcaggttctg gatattttca actttttcgg tttttaattt ttgataggct tttaacagtg
      721 tcgtgtataa ttcttgttct gcattgacat aataactatc gagttcttca aaaattattg
      781 aaataccact ttctgtttcg gcttttttct ttgcattatg aatagtctgg acagtttttt
      841 gagcttcact gtaacttgaa gaaaatgttt ttattaaatt taatactcgt tcagggataa
      901 acgtagcaat gctgtcgttt gtcataattg tctcaaaatt gtctaacaac aattgatctt
      961 gtaggcggtc tttttcagtg tctatattgt gtctattagt gtctaatata ttattattaa
     1021 gttctggtat gattttcata ccctctgatt tatcaacgtt ttggcacggt atgattttca
     1081 taccctcttg agccccaagg gattcagcgt ctgatttttc gtcgagggtg ggtatgattt
     1141 tcataccctc tgatttatca acgttttccc tattctctct atcaaatttt tcgagtaagt
     1201 agatatcatt ttctgagact tttaattctg ctagatatag tctattagga ttatttttcc
     1261 ctaactgttt gttaaatccc atctgctttt gtaataataa accaaaggat tccaactctt
     1321 ttttgattgc tatcgctttt tgtttttggc agtttaatac gctacatagt tcatttgtcg
     1381 tataagtaaa ataaacatta ccttcttcat cgatttgccg tttgctgatt gcgatctcta
     1441 atcttgcttt taaaagcata taggcaatct tagttgaatc tttcattttt ttgtattcgt
     1501 cagaaacaat aaagactttt ggaaattgat aaaataattc gttgtaaact ctatccgcct
     1561 taaaaaaatt aaaatcagtc attgtagatc ctctactttc acattgtttt atttatgtaa
     1621 aattacctac tcttttcgaa aatagcaaag ataaataaaa aatgcaaacg cattaaaact
     1681 gttttttaat taaatactac ctaaaatcag aaataaaatg aattatgtac tacctttaaa
     1741 gtcattaaga actactttaa ataaatatta tttataatta aatactacca gaagaacgta
     1801 tgaataaaat ttagttttaa aattaaagat aattaagtac taccagtgtc aaaacaaaaa
     1861 ataaattaag aactacctta tgcatataaa acatactacc attactagta atatatacta
     1921 ccttatttca gtagtatata ttgtattctt cttataagac aaggctttag gctagaaaac
     1981 aatagattat atactactaa aaaatacaat aagtactacc aatgtcagat atatataata
     2041 attatgtact accattaaaa ttaacttatt taataagata ctactgtgtt aatctgaaaa
     2101 aaattacttt aaagtagtta catactacct gttaaattag atactaccat taatacttaa
     2161 attagatact acctatattt atattaagta ctaccgttct aggaataaga taataaaaaa
     2221 aaactaccaa attgcaatta aatactactt aaaatgtaaa aaataagaac tacccaaatt
     2281 aggtagttct taataagaga atatcagata aatttgtaca agtcataaaa aaattaatct
     2341 taaattttta aatatatata cctgttaatt ataaactact ttatttaggt agtacataat
     2401 taacagataa aactacatgc ttgtagtttg tctagctaat gagttgcata tgatttatat
     2461 aaaggtgtgc agcataactt agttgaagtt aagagaatag aataaatatt ttttgttttg
     2521 ctaataatta aacactacct aaatatggta gtgtttaatt atataatatt cattttgtta
     2581 caaataatcg tattataaaa gcataacaat tgggataact attttttaga ttatttaagt
     2641 actacttaaa ttaggtagta cttaaatttt ggtttaaggt agtaggtaat taagaaataa
     2701 tatatatctt cttttataat agaaggggag aggcctagtt aaaagcagcg acaattaata
     2761 acaatgtatt taatgcttca caaaatccat ttggtggaca atgcattgtt attagcgatg
     2821 atctaccgtt ctaagtagtt ttttctattg gttgcattcg tgactaatta tggtataatt
     2881 atgagtaaca aagaggtatt tcattatatc atcaaacccc atcagcgtgt aggctgatgg
     2941 ggtttttttt acgtttctat attatagaat taactggtag gcttactgtc ttttttggca
     3001 attttgcaat caagccactt gcagatgtag tgtgcagtta cctctgcaaa tggcttgatt
     3061 gcaaaaacaa aatcaaaaag gtatttcatt atatctcctc ctccccttgc cagattaggc
     3121 cggggtagca gacagcaaaa ctattataac ataactgaaa tacctaaatg acatatacag
     3181 atatgataat ttatgtattg atttttctaa caaaagcaca tattttaata tatactacct
     3241 attttaggta gtatatattt ttagatacaa tatcgatcag ttttgaggaa atgaaattct
     3301 agatattttt tgctacatag agttactttc aatatttttt aacgtaatta ggcagaagtc
     3361 actcatcctc tataggtttg tgatgaaatt gctattgatc catttaagta agagcaaaaa
     3421 actggaggtt tatcaggtaa ttggtctaat ggatttaagt atgcggtcac cgtgtataga
     3481 gttgcttatg agattcaaga aaataaagtt attccagtat tgctatatgg aacacataac
     3541 aatttttatg aattagccga gaacactcgc aactttattc gtgtagatga atcggcttcg
     3601 gttgagttat aactttagct atatcaatca accgaccgac atatgttaaa tctatatata
     3661 ataaaaatac ctacttaaat ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat
     3721 tttgatatcg tttgtatatg ttttcagcgt tctaagtggc ttattttgtc caataataat
     3781 atggagtgga atcccttaag aaacaccgtt ggcaattgtc gttataacat gttataattt
     3841 atttatcaaa aactaaaaga agtgtgataa catgggaaaa gaactaaagg ttagaaagat
     3901 cggcaatagc gtgggagtga tccttccaag ctctcttgga ttaaaaagcg gagacactat
     3961 ccaagccaaa caagaaggca acttaatcat attagatact actcaaatag caaaagaaca
     4021 cgatcgcaaa ttgattgaag aaagtttcca agactttgag aaaggtttaa cagtttcaga
     4081 aattgaaatg gtaaaagctt tcggaaaata cggctggagt gaatagatga aagagtacac
     4141 gctagtttat tctaaaagtt tccagaccag ccttgaaacg ataatagaag aatgggaaaa
     4201 tgagcttgct ctttctccag ccagcataaa aagatttgta ggtgcgatcc agaaggcttt
     4261 agaactaacc aaaaattttc caactatgca tgaggaagtt tccgaaatct atggaatgga
     4321 tatcccaaca tatcggatcg tgatcggcaa aagctatgcc ttattttatc ggatcgacca
     4381 tgaaaaaaaa gagatacttg taggacgtat tttccaatct aaacaaatga aactaggctt
     4441 ttaacaataa ttaggcaaaa gtcgctcatc ctctacaggt gggtgattaa tgccgctcga
     4501 tatggttgtt aaccgtcggt tactcgaaga ttgatatata agacttagta cctttttatt
     4561 ttgctccatg ttatggccga aggtctaaag atacaatcat tagtctagag aaaaattaag
     4621 aaccgtagga actacgggga tcgcttggtg aataagagac actgctatta gtgaaaaaat
     4681 aagatatcaa gtacgctcta ttcccaaaaa tttcgtgact ttactcatga gaggttcaag
     4741 tagtatattt ttaatcaaaa ttgacgtaac tttta
//
LOCUS       DACEKQ010000138         4772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEKQ010000138 DACEKQ010000000
VERSION     DACEKQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.146"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..1398
                     /locus_tag="IW671_002624"
     CDS             7..1398
                     /locus_tag="IW671_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0899577.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IW671_002625"
     CDS             1414..2454
                     /locus_tag="IW671_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0899578.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IW671_002626"
     CDS             2473..3558
                     /locus_tag="IW671_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0899579.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IW671_002627"
     CDS             3591..4553
                     /locus_tag="IW671_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0899580.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..>4772
                     /locus_tag="IW671_002628"
     CDS             4546..>4772
                     /locus_tag="IW671_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HAQ0899581.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGA"
BASE COUNT         1739 a          645 c          858 g         1530 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca at
//
LOCUS       DACEKQ010000139         4617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEKQ010000139 DACEKQ010000000
VERSION     DACEKQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.147"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..962)
                     /locus_tag="IW671_002629"
     CDS             complement(15..962)
                     /locus_tag="IW671_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0899582.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IW671_002630"
     CDS             complement(1140..1943)
                     /locus_tag="IW671_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0899583.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IW671_002631"
     CDS             2089..3003
                     /locus_tag="IW671_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0899584.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IW671_002632"
     CDS             3051..3521
                     /locus_tag="IW671_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0899585.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IW671_002633"
     CDS             3606..4214
                     /locus_tag="IW671_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0899586.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IW671_002634"
     CDS             4348..4548
                     /locus_tag="IW671_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0899587.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1512 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttaga
//
LOCUS       DACEKQ010000140         4487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEKQ010000140 DACEKQ010000000
VERSION     DACEKQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.148"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(462..887)
                     /locus_tag="IW671_002635"
     CDS             complement(462..887)
                     /locus_tag="IW671_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899588.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1261..2232
                     /locus_tag="IW671_002636"
     CDS             1261..2232
                     /locus_tag="IW671_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0899589.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2264..3661
                     /locus_tag="IW671_002637"
     CDS             2264..3661
                     /locus_tag="IW671_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0899590.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3820..4443
                     /locus_tag="IW671_002638"
     CDS             3820..4443
                     /locus_tag="IW671_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899591.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1570 a          719 c          916 g         1282 t
ORIGIN      
        1 aaaaattact acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat
       61 atttgaaata aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt
      121 ttactgatga agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat
      181 agtgagtttt gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat
      241 aagattcaaa tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca
      301 agcttccttg gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat
      361 caggactatc gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca
      421 gaaaagaagg ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg
      481 ccatcctcat ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt
      541 ttattttctt ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc
      601 agcactgttt tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt
      661 gtccattcca tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta
      721 cgtactttta gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa
      781 gaatcggttg tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat
      841 gtcaaaagtc ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca
      901 tttgatagga taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa
      961 aaacgcacaa aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa
     1021 aatccgccac tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat
     1081 attaaaaatg aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc
     1141 tctactgaag actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg
     1201 gtcatcataa tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt
     1261 atggcatcga tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt
     1321 tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca
     1381 gcgttggact ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct
     1441 attggacttt tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc
     1501 gaaagagtag ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca
     1561 gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc
     1621 ttcttatccg aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt
     1681 atcgcattga attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga
     1741 gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt
     1801 attgctgcca ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg
     1861 gtccaaaatg atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc
     1921 ggctattacg gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat
     1981 tacaatatgt cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag
     2041 gatattgcgt tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa
     2101 atcacgatca cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa
     2161 cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat
     2221 ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt
     2281 tccagcaaat ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc
     2341 aggcgatgga aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt
     2401 ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt
     2461 acagttgatg aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt
     2521 gatacctgat gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt
     2581 tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc
     2641 gatgtatctg caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa
     2701 atatgccaaa agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa
     2761 tgaaccaatc gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc
     2821 agatatgaaa aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat
     2881 caaatcttac catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc
     2941 aagctatcca agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc
     3001 gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat
     3061 tgagatagta aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat
     3121 caaagaaaat acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc
     3181 aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta
     3241 tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat
     3301 ctatgatatt ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga
     3361 aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga
     3421 tgattatcgg atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga
     3481 aggtgccaat tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa
     3541 tgcatataag aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac
     3601 aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta
     3661 agaaagaata gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag
     3721 aagttcaatt atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata
     3781 taatagaata gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt
     3841 cttctctttt ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga
     3901 aaaatgacag cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca
     3961 gtagataatt tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc
     4021 aattacgaaa aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt
     4081 attaatatcg atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag
     4141 catttgaccg ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga
     4201 aaagtgatca ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg
     4261 gaatggaaaa agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag
     4321 tcgacaggaa caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa
     4381 aatcaagcct caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa
     4441 tgaaaaacag aggttgtgaa atcagcgttt tgacctgagt attagag
//
LOCUS       DACEKQ010000141         4487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEKQ010000141 DACEKQ010000000
VERSION     DACEKQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.149"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW671_002639"
     CDS             544..1107
                     /locus_tag="IW671_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899592.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW671_002640"
     CDS             complement(1386..1673)
                     /locus_tag="IW671_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0899593.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW671_002641"
     CDS             complement(1663..1992)
                     /locus_tag="IW671_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0899594.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW671_002642"
     CDS             2297..2626
                     /locus_tag="IW671_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899595.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW671_002643"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW671_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0899596.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW671_002644"
     CDS             complement(3551..4462)
                     /locus_tag="IW671_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0899597.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1486 a          754 c          666 g         1581 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatata
//
LOCUS       DACEKQ010000142         4463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEKQ010000142 DACEKQ010000000
VERSION     DACEKQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.150"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..1229
                     /locus_tag="IW671_002645"
     CDS             24..1229
                     /locus_tag="IW671_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0899598.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1245..1889
                     /locus_tag="IW671_002646"
     CDS             1245..1889
                     /locus_tag="IW671_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899599.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSSAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVNEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            1900..2706
                     /locus_tag="IW671_002647"
     CDS             1900..2706
                     /locus_tag="IW671_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899600.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMIIGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            2729..2958
                     /locus_tag="IW671_002648"
                     /pseudo
     CDS             2729..2958
                     /locus_tag="IW671_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3117..4052
                     /locus_tag="IW671_002649"
     CDS             3117..4052
                     /locus_tag="IW671_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0899601.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            4096..4365
                     /locus_tag="IW671_002650"
     CDS             4096..4365
                     /locus_tag="IW671_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899602.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT         1602 a          657 c          951 g         1253 t
ORIGIN      
        1 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg
       61 ttaagcgtcg tgcaaagcga cgcttatttt tgtggttgtt tggtacttct gcaggtttga
      121 ttactatttt gatcactgtg ttcgtgaccc tttttctaat actggcggcg ggttcgattg
      181 ataattctga ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag
      241 gattacccat ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat
      301 atgctgtagg tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg
      361 gttacgaatc agcgtttggt acgtcggcgt ccgcaagaaa tgatctcaac tattttggga
      421 ttacgtggtt cgatggctgt ttgtttccta aaggaactgc acgaggaatt ggtggtattg
      481 aaggtggctg gtacatgaat tttcccaact caaaagctgc attctcctat tacggcttta
      541 tggtagccac acaatcaaat tttaatgcat gtgtaggaaa taaaagccct ggcgcaagct
      601 tattgatctt aggacgaggt ggttatgccg ctgcaggaat tactgaagat agtccgtatt
      661 atacaggttg tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcga
      721 tgaaacattg gggtgaaggt ggtaatgata atggcacaat tactggtgaa tggacgaatc
      781 cattccctgg cagcagttta gataaaagta cctttagcgg tggtcaatta tttggaacaa
      841 atccaggcag tgaattccgt ccgaatggct tccatgatgg attagatttt ggttcagttg
      901 atcatccagg aagtgaaatt cacgcggtgc atggaggaaa agtcgtgtac gttggcaatc
      961 ctggtatttc aggtttaggt gcttgtgtga tcgtgattaa ttacgacggg ttgaatatgg
     1021 tatatcaaga atttgcgaat agtacaggaa attctcgtgt gaaggttgga gatcaggtga
     1081 aagttggtca agtaatcggg atccgagata cagcacactt gcatttaggt ttcactcgaa
     1141 tggattggcg tcaagcacaa ggacacgcat tcacagatga tggtacatgg attgatccat
     1201 taccattttt gaatagcagc aaaaaataga tcaggagaaa aaatatggaa aataaaactt
     1261 taaaaatcgt cttaacagct gcaattagcg ttagtttaat cggcaatatc gcgttgtttt
     1321 ggcagctgaa agaggaacaa gcgacaaata cctataaaat tgaagaagtg gctaaaaacg
     1381 cgtctgagaa gctcaaaaaa aattcctctt cagcgcaggc aaagatcgac aagttaaatc
     1441 aaactattaa tgagaaaaac caagaaatcg atcagctaaa gcaacaagat ggcacaaaag
     1501 caaaagaaga agtaaatgaa gcacaaagta agacggcaga agagttcggt aaattggcca
     1561 ttgataaatc attaagcaga aatgaattaa cggaaaaact aaaagatgta gcaacccaag
     1621 aagtgattga caaattaagt cctgcagacg atgatcacca acatgatgat tacggaagtt
     1681 actcctttac attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac
     1741 aaaattttgt cgtatttgtt gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac
     1801 cgcaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag
     1861 tcacagactt tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagca
     1921 agacaacgtt tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattatc
     1981 ggctttttaa ttcttgctag tccgatttat acgcataaga aggtagacat accagatgag
     2041 gtaagcacga acgcattttt aaacacgaag gaactgacga tggtttcaaa agaaaaagga
     2101 gcgaaccagc aattagtttt tgaatttgcg attgacgatg aagatcaaag cttattaaaa
     2161 gaactggcta atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca
     2221 ataaaagcca aagtgattaa ggtgtctgat gattattttg cagtgaagct atcgcatgta
     2281 ccagaagaat atattgctat gcgattaact attattcctg aaaaaattga tccaaaggtg
     2341 gatatgcaag aaccgcaaga tctgattttt tatattcacg aagataaggt caaagatcac
     2401 gtgaaagacg aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa
     2461 gaacaaaaag cagcacaaaa acaaattgaa tccttccaag caaccattga tttgaatgaa
     2521 gaattagtga agaaactaaa agatcaattg ccttatcaag tcgcagaaga tcaggaaaat
     2581 actgaaaata agattaatag ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa
     2641 gatcaagaag agagcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaat
     2701 atttagaaga ataggtggag taaaagaaat gaagcgagcg aacttggtcg gcaaagatac
     2761 tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat
     2821 atgtcaagaa gtgaattatc taacacaaca atatctgagt tgaagaatgg tagaaagagt
     2881 ttagatacct taaaattcag atcatttgaa gctctatata tagctgctaa aaaatatttt
     2941 acagctgata ataaataaaa aattaaggtg ggttctatgt gaaaaaaacg tatcaaaagt
     3001 ttttatggat tagctatatc gtcctattta tcgttgggtt tctcgtcagt gtgtccgtga
     3061 tcgtcggaac gctttttctt ttgttctctg acgaagatct ggtacttaag gggttaatga
     3121 gtacgttatc gtgtgtcatg ttactttatt tatgtataca gatcaacgaa attatgcccc
     3181 ctcattggca agaaaagaag aaagaattta aggaagaaat cgaaataaaa tccaatgact
     3241 ggattgaata cacatggctt cctctgaaaa gaaatagcaa acgaaaggaa ttttgggcat
     3301 tgcaattatg gccaatagga aaacttttta tttatatggc ttcacttatg gttactttaa
     3361 tcggttattt ggtggtggtc tatggggtgg cctattactt gttaagtgag ccaaaacaga
     3421 tcgcggcgtc aaatattttc acggcagtaa tgacattaat gtgtgtcatg tgcttttcta
     3481 tttttagtta cttactcgtc caatttatca ggcaaaattt aacaatgagt aaacttttca
     3541 aaagtttatt ggacttactt atcttaggca gtatgttaac gactctaaca gatcggttcg
     3601 aacaatctga aaaaacggta gaggggataa agaacttctt aacgacgatt gatcaattta
     3661 taaatggata tatgtatttg tttgaagtag gctttattct cactttgttt ggttgtgtat
     3721 ttataaaact cattaaaaga tcaatcgaaa aagaaatggt tattgatcta attccgacgc
     3781 tgagcggcta tgagtattac gttgattttg gaaagtatga ggagttgatc catacggata
     3841 tcgttcacac gagtatccat gaagtaaccg ttaagggcat cagatctact ttaaaagatg
     3901 gacaggagac gaaaacaatc gaccgaagaa aagaggtcta caatttttat aaagttgaag
     3961 caagtctacg atatggcatt gggaaattga ggttagatgg gagtaatatt caacaaaaca
     4021 aaccatatgt tttaggaaaa gtggtgattt agaacttcct atgaaagatg aataaataag
     4081 gagaatcttt tgaaaatgat tcttctttac agaattgtgc ttatagttgg cacggagtta
     4141 aaaatcttaa agaaggagca gttattcaaa gtgaaagaac atcataatca gttactatgt
     4201 attaaaacgg gtacgatgaa cattcaatac cgaacagaat atttagaatt aacgaatcaa
     4261 actgattttt tgtcgattcg agagcaacat aagcaagaaa aattaatggc tacttatagc
     4321 caattgattg atgagtggga acaattcaag gaatctaaaa cataaaattc catttaaaca
     4381 ccttattttt taaagagcat aaggaaacgt gaccccactt ttaaaaaaag tggttggata
     4441 ggctgtgcct atcacgtttc tga
//
LOCUS       DACEKQ010000143         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEKQ010000143 DACEKQ010000000
VERSION     DACEKQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.151"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW671_002651"
     CDS             complement(543..2048)
                     /locus_tag="IW671_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0899603.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW671_002652"
     CDS             complement(2045..2731)
                     /locus_tag="IW671_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899604.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW671_002653"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW671_002653"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0899605.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACEKQ010000144         4394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEKQ010000144 DACEKQ010000000
VERSION     DACEKQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.152"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1130
                     /locus_tag="IW671_002654"
     CDS             <1..1130
                     /locus_tag="IW671_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0899606.1"
                     /translation="HTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQ
                     VLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGKESDGKEKEEEP
                     LLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSEKAQSILKEVEG
                     IGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESY
                     LLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHL
                     GICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKG
                     LKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            1369..1962
                     /locus_tag="IW671_002655"
     CDS             1369..1962
                     /locus_tag="IW671_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0899607.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1978..2877
                     /locus_tag="IW671_002656"
     CDS             1978..2877
                     /locus_tag="IW671_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0899608.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            2880..3365
                     /gene="ssb"
                     /locus_tag="IW671_002657"
     CDS             2880..3365
                     /gene="ssb"
                     /locus_tag="IW671_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0899609.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            3387..3644
                     /locus_tag="IW671_002658"
     CDS             3387..3644
                     /locus_tag="IW671_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899610.1"
                     /translation="MAKKNSLTMDLLIPVIAFIDGISLGITENLLVNRIAKGSLFLVF
                     VYLVCSYLLKRVGKDNSFWRLTKVTIGATAVILTAILPLYM"
     gene            complement(3804..3926)
                     /locus_tag="IW671_002659"
     CDS             complement(3804..3926)
                     /locus_tag="IW671_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0899611.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            4080..4340
                     /locus_tag="IW671_002660"
     CDS             4080..4340
                     /locus_tag="IW671_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899612.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLHTSHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
BASE COUNT         1619 a          742 c          893 g         1140 t
ORIGIN      
        1 ttcatacgcc gaatagtcgg tatgtgaaca ataagaaagt ccaggaacat tacgcgatca
       61 ttcctacaaa aacggttatg acagcagcgg cttttgaaca attaagtcca ctgcagcagg
      121 ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag tatacctatg
      181 aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaagcaatc ggaaaagtgc
      241 cggtagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa gagaaggaag
      301 aagagccgct acttcctaaa gtggcaaaag gagaaatggt gacggtagat ctacaagttc
      361 ttgaaaaaga aacaaagcca ccaaaaccat ataccgaagg tacgttgatc acggcgatga
      421 aaacagctgg gaaaaccgtg gatagtgaaa aagcgcaatc catattgaaa gaagtggaag
      481 gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa aaggaatata
      541 tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt tgccaagccg
      601 tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa agctacttgt
      661 taaagattgg agaacgaaaa ggaacacaaa ccacttttct agccaatatc caaaaatttg
      721 tctctcactt attggaagtc gtcccaggac aaatccaatc cactgatttt ggatcgacgt
      781 tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta ggaatttgtc
      841 caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt acgtctgaag
      901 cctgtgattt taaattatgg acaacgattg ccaaaaagaa actcacagcg acacagctga
      961 aggagattat tcaaaatagc cgaacaagcc aaccagtgaa agggttaaaa ggtcagaagg
     1021 gttcgtttga agcaacgatt gttttaaaag aagacttcac tacaggcttt gaattttctg
     1081 aaaagaaaaa aacgaacttc aagaaaagaa cacgacgcac tacaaaataa tggagggtta
     1141 atcttatgcg taatacaaca cacaaacaac aagaaatcga aaatcaaaaa gccaaaattc
     1201 gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaatta aagaaccagc
     1261 aacggattca aaaagaaatt gaattagatc gataaatatc tttcttgtta atataaaact
     1321 aacgtgatat agttatgtta gttttatatt aactttaggg gaaaagcaat gaaagacaaa
     1381 atccagtgca tttttgataa acataatggg aatctatcct tgaaggatgc tagaagtgaa
     1441 ggcttatcgc ctatgacgtt agatcgatta gccgaaaagg acaaaataga aaaggtagca
     1501 ccaggtttct ataccttaaa agaagcgttc gtggatgaat tgtatctcgc tcaaagtatc
     1561 tattccagag gtattttttc tcatgagact gcgctcgatc tttttcaagt cggaacgtat
     1621 atccctaaaa agatcaatat gatgtttcct aaaggataca atgtttcaaa ggagaaatta
     1681 gacgagtatg ctattcaacc gcattacacg aataaagaaa attttgacct gggaatcaca
     1741 gaaacagaaa gcttttacgg caataaaatt ttagtctatg atttagagcg aacgttatgt
     1801 gatatgtgga atccaagata caaagcaagc gtagaggtaa aacaagaagc attaaaagaa
     1861 tatatgacaa gtaccagtag aaatacaaat aagctaagaa gatacatgga tattttaaaa
     1921 tcaccaaaag aaatgacttt gtatatgttg ccactttatt gagaggagta aaggtgaatg
     1981 acaccgacac aatttaaagc aaaaacgaga aatttcgcaa cagagaaaca tttaaatcca
     2041 caaattgtcc aaagatatta tatgatggaa aaattattag aacgtattgt tgaatcgcct
     2101 tatcatgatg attttgtatt aaaaggggga tttctgatcg gatcaaaata cgggttagaa
     2161 aaccgttcaa ctgttgatat tgatacgact tatcgaaatt cgaaactaac aaaagaaaag
     2221 ttagaaactg ttttttctga gttaacagct gatcccacaa aagatggaat tcgatttgaa
     2281 ttaaagggat taacagagac gagggaagcc gattattacc caggttttca agcaaagttt
     2341 attgcttatc ttgagaatgc acgtgtacca tttaaattag atattacttc aggtgattct
     2401 attattccag cggcattgat gtatgaacac aaattaatgt ttgaagataa aacgattcat
     2461 attccagctt atcctacgga acaaattcta gctgaaaaat tacatgctac gtttagtttc
     2521 ggaaaagata attcgcgcat gaaagacttt tatgacctgt atatgattcc aaaactagaa
     2581 cgtatcgatg agaaaaactt gtataagagt gttcaaggaa cgttcaagga aagaaataat
     2641 caatctgtgt ttaaaaaatt gtataatcag gaaatgcctc aattgatgaa ggatgagggg
     2701 ttgagggaaa agtggaaaag ataccaacaa gaaaattatt ttgccaaaga catttctttc
     2761 gaatctacac taacttccgt cgattctttg atgcaatcaa ttgtagagca agagaaagta
     2821 gaacgaaaac aaaattttaa gagatcacaa caaatgaatc aagaaatgga gagataaaaa
     2881 tgattaataa tgtaacttta gtaggacgat taaccaaaga tccagattta cgttatactg
     2941 caagtggtac tgcagtagcg acttttaccc tagccgttaa ccgaaatttc acgaatcaaa
     3001 acggcaatcg agaagctgat tttatcaatt gcgtgatttg gcgcaaacca gcagaaacga
     3061 tggcgacatt agctaaaaaa ggcagcttga tcggtgttgt cggacggatt caaacacgga
     3121 cttatgacaa ccagcaagga caacgtgtgt atgtgactga agttgtagca gataactttc
     3181 aactattaga aagtaaagca gcgacagaga gccgtgcgca tgcagatcaa tcgtccacca
     3241 gtccaagcac cgccaccttt gaacaacggg atacagcgac gcctaacaac aatggattga
     3301 acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgatgat gatctaccgt
     3361 tttagttcga gaaagaagga gtaagcatgg caaaaaaaaa tagtctgacg atggatctat
     3421 tgatacccgt aattgcattt atagacggga tttctttggg tatcactgaa aatcttttgg
     3481 taaatagaat tgcgaaagga tcactatttc ttgtattcgt gtatcttgtc tgttcttatc
     3541 ttctaaaaag agtaggaaag gataattcct tttggcgatt gactaaagtc acaataggcg
     3601 cgacagctgt tatacttacg gctattctac cgctttatat gtagggttaa cgggaaatca
     3661 ttgaacttct tgcgaaatat tgttataatg aacatgaaaa agaggagttg cgcaaacaac
     3721 ccctcattaa cagcaccgtt taaagaacgg cggctcagat taattattta ttgagaaata
     3781 accgatcctc gctaaagtga cggttatttc tttttgtcat ttttaagcat atttacaatc
     3841 aaactaatca aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat
     3901 gcggacaaaa ggcttactcc tttcatagat tttagcactt acattcataa gcaccaccac
     3961 cttccagaag tcgccaccgt cattaaactt tactgtcttg atcattatag catatctaaa
     4021 atcgaataaa aattctcaat tttagctgtt tttgctataa ttttagcgag gtgatcgcga
     4081 tggaacatta tactggttta ctcgataaaa ttcgggtgat caagacacag ccattgcttg
     4141 tgcgatttac attacacacg agtcatgaac caatcaattg tgtagtggcc aatatagaaa
     4201 tagcggataa attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact
     4261 tcaataaacg gaatcaatta gtcattgaat cgatgcaaat tagaaacccc gatcatttta
     4321 cgcgatcgat gggcatataa aagaagagct gcaatcctaa tgtaggactg cagctctttt
     4381 tgttttacat agaa
//
LOCUS       DACEKQ010000145         4305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEKQ010000145 DACEKQ010000000
VERSION     DACEKQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.153"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            148..1137
                     /locus_tag="IW671_002661"
     CDS             148..1137
                     /locus_tag="IW671_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0899613.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IW671_002662"
     CDS             1134..1553
                     /locus_tag="IW671_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899614.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IW671_002663"
     CDS             1562..1870
                     /locus_tag="IW671_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899615.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IW671_002664"
     CDS             1874..2236
                     /locus_tag="IW671_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899616.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IW671_002665"
     CDS             2458..3864
                     /locus_tag="IW671_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0899617.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACEKQ010000146         4299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEKQ010000146 DACEKQ010000000
VERSION     DACEKQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.155"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW671_002666"
     CDS             <1..156
                     /locus_tag="IW671_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0899618.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW671_002667"
     CDS             175..1452
                     /locus_tag="IW671_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899619.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IW671_002668"
     CDS             1462..2118
                     /locus_tag="IW671_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899620.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IW671_002669"
     CDS             2118..3494
                     /locus_tag="IW671_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899621.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4299)
                     /locus_tag="IW671_002670"
     CDS             complement(3780..>4299)
                     /locus_tag="IW671_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ0899622.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1681 a          505 c          750 g         1363 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg aagatgttcc
     4261 acttcatcca tcagatctcc ccaaacatgt ctcgtgatg
//
LOCUS       DACEKQ010000147         4177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEKQ010000147 DACEKQ010000000
VERSION     DACEKQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.156"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..200
                     /locus_tag="IW671_002671"
     CDS             <1..200
                     /locus_tag="IW671_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899623.1"
                     /translation="NIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTI
                     LAVSLLLLVLATFGYAVLKFN"
     gene            complement(236..2293)
                     /locus_tag="IW671_002672"
     CDS             complement(236..2293)
                     /locus_tag="IW671_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0899624.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2437..2628)
                     /locus_tag="IW671_002673"
     CDS             complement(2437..2628)
                     /locus_tag="IW671_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899625.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            2816..3271
                     /locus_tag="IW671_002674"
     CDS             2816..3271
                     /locus_tag="IW671_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899626.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            3268..3510
                     /locus_tag="IW671_002675"
     CDS             3268..3510
                     /locus_tag="IW671_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899627.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3528..3830
                     /locus_tag="IW671_002676"
     CDS             3528..3830
                     /locus_tag="IW671_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899628.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGIILAAASIVVSLFGMFGQTPQGL"
     gene            3899..>4177
                     /locus_tag="IW671_002677"
     CDS             3899..>4177
                     /locus_tag="IW671_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899629.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGF"
BASE COUNT         1293 a          671 c          837 g         1376 t
ORIGIN      
        1 aaaatattga taaccaagcc gtagtagcag tagataatgt ttctaataaa acaaatgttg
       61 tgacaattgg ggttgatcct aacttgccac aagttattgt tcctaaaaca ggttctacac
      121 atttagtaac gattttagcg gtcagcttac tattacttgt gttagcaact tttggctacg
      181 cagttctgaa gttcaattaa acaaaaaaac aaggctgaaa atcagccttg tttttctatt
      241 tgaaaatggg attggcacta ataaatacgt tgtctgaaga aagtgatccg tctgcagaaa
      301 cttcataagc atgggataaa ccaacgatac tttgattgtt gtaaagcaag tttgcgttat
      361 gagcagcgct atctttccac ataaccatag catttactgc tcctgtatct gaaaggatct
      421 cgtcaacgta aaggtagaaa ctattgatat tttctccttt agcaccgtag cagcctaagc
      481 tttctaagag atcttggaaa gtaccgccgc caggtcggta atgactgaag cttacttgta
      541 attcttttgc acgaatatct gcagcttgtt gaacgatatc attgaattgc attggggcaa
      601 cgccattttg ttgacggtag ctatttaacc aagcaagttc tttttgacgc acatagttgt
      661 ttaaatcact tagtgagata tttttgtatt tgaaagtgat cgttcgattt tggtcaacat
      721 tattgattac acgttgtcca tttttatcaa cggtaacaga tgaatcgtca tttctacaca
      781 agaagtagtt ttgatcggtc atatttttcc aagaagtaga gaaactatct ccctcgtaaa
      841 ccgtttgtgt ttcagtagat aaaacagcgt ttgtgtcaac gtttatatgg ttcacagtga
      901 cgttgtattg atgagtatag gcaaaattga ttccttgatt acttgtaacg ttgtttaaag
      961 tcacagtgtt gctagagctt tctctaggat caagaacaaa attagtgaac ataggtgctt
     1021 caattgtgat gctttgtcca taaacgacgg gataactctt actgtaatta tagagttctt
     1081 ggatatttgg atcagtatcc gagactttat ttggacgagc tttcacttca actagaaaga
     1141 cttcatcata gtaaaaagta aatgtggtat ttgtgttaat cgtttgtgtt tgtgtttcat
     1201 tcccatttac ttgatactgt ggtgcttcaa agttagtcaa gtcggttcta gcttttgcag
     1261 tgtaacttgt accagctttt actgagacat gttcttcttt tagcagcgtt ccagtttgtc
     1321 gattcatatg acggacagtt accgcatatt ctgaaggttt aggtttgtgt tgtaaccctt
     1381 taaatgcaga atcaagacta tttttagcat tatttacttg ggtttgagaa gctttagaat
     1441 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gatccttctg
     1501 taaaatctcc tttagcagtt gattttactt ggttgtacaa ggcttgtaat tccgttttgt
     1561 tcacagtagg agctggtttt ttctttaatc ccttgtaagc agaatctaat gtgtttttgg
     1621 cactatccac ttgtttttga gtcgcttttg agtcatttaa cactttttta gcattgttta
     1681 aagcggtttg gaatgcgttc caggtattgt cggtatagtc acctttttta gttcccttaa
     1741 cgctcgtata aagagcttct aaagctgatt tattcacttt gggtttagag gtgtcagcac
     1801 cataccaacc aataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
     1861 tccaacttgc agtgtagtga tgcgaaccag aggtaacacc tggaaggaaa aggcggtaga
     1921 tcggtttatt atttttttcg gcagaacgcc atgcaggccc ttcataagtc caaccgtaat
     1981 ttttggtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaaggcctt
     2041 tattgtataa acgatagaca aggtggccac cgctagtcgg tgcttcccaa gcggggcctt
     2101 cataattacc ccacttaatt ttcactaggt gatcacgttc ttttgtgcta gaggtgtaaa
     2161 aatgttcatg gttatttgga ttataaaggc gatacatgac gttagttgaa gcagcgtcag
     2221 cagatccagc aagagcaact gtggcagcaa atgttccaat agctataaca gtttttgggt
     2281 ttaataattt catgttttca actcctatta aatgattaaa tattttgttt acattcataa
     2341 tataccacgg tataccgagg tagtcaacgg tataccgtgg aaattttgaa ataaaaaagc
     2401 cgccctatga agagtgtaag gatggctgtt tatatgctat gaagaaatgg attcggcgta
     2461 tttggttagt ttggcagcat tatcaaatcg cattttttca atcgcacttt ttttctttat
     2521 taaatccgca attgttgatt ctgcgactga agaatgtttg ctaatttcat atgctgtctt
     2581 gccagtttca aatagtaacc attcgatttt ctctttatca gcgtacattg caatgctccc
     2641 tcttgatttt ttctttatta tagctctttt ttgtaataaa tcacaatatc aagaggaaaa
     2701 gtttctatag aaaggtggga aattatgagt atccgttccc cgtgtatcaa aatgaggttt
     2761 gttgctactt tcgttatttg atatactaaa ataaaacaac gaaagaagga atagaatgaa
     2821 aaagttaata atggtattta cttcgttgat tgccatgatt gttattttag gtggatgtgg
     2881 tagtgaaaaa aataagcaag atagtcaaga taatggcgtg ttaaaaccag gaacagtgtg
     2941 gaaagaagaa gtaggcggac ttgttcataa tttaaaaatt atagatgata caacatggga
     3001 atattccgaa agtgtttggc atcctgaaca agtacaaata acagttgaga agctaaaaga
     3061 ctataaagga ttagaaagat ataaggtaat cgattcaggt ggagtagatt tattcatagg
     3121 taaaacagcc ttaatagttc cctataacaa agacggttta gaaacgttta aatttattcc
     3181 aacaaacgaa aaagaacatc gagataaaag tgaaatatta aaagaataca gtactaaatc
     3241 tggatacaag ttacaaaaga ctagcgagtg attgctagtc tttttattta tcctgaagga
     3301 ggaatcacag tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa
     3361 cgtcgcggtg tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac
     3421 ctattacggg gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag
     3481 cttgttttcg atgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa
     3541 ttacaatgaa ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat
     3601 tacaaactgc agcaactagc ttaaaaaaca atttaatgat ttttgccggc gttgcggccg
     3661 ttgcttccat tgttattggg gggcttttct tcttatttgg acgaagaggt gctgaaaacg
     3721 ggaagatgca tgtgggatat gtattgatgg gggtaggaat tattttagct gccgcttcta
     3781 ttgtggtttc gttatttgga atgtttggac aaaccccaca gggattataa aaacaaaatg
     3841 aaggagtaaa aagtacatag ctacttttta ctcctttttt tatgaaagga gaatattcat
     3901 gttttttacg atttcaggca atgcaaaatt ggataaagag ttaataagaa aagcaacgaa
     3961 gaaagtggga aagcgtactt cctttttacc gaaaacaatt aaatattctg tcattgaaac
     4021 ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa
     4081 attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt
     4141 ttttggccca ctggattatc aaatttcaga aggtttt
//
LOCUS       DACEKQ010000148         4116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEKQ010000148 DACEKQ010000000
VERSION     DACEKQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.157"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            279..1127
                     /locus_tag="IW671_002678"
     CDS             279..1127
                     /locus_tag="IW671_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899630.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="IW671_002679"
     CDS             complement(1298..1639)
                     /locus_tag="IW671_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0899631.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="IW671_002680"
     CDS             1725..2432
                     /locus_tag="IW671_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0899632.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="IW671_002681"
     CDS             2419..2742
                     /locus_tag="IW671_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0899633.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3865)
                     /locus_tag="IW671_002682"
     CDS             complement(2831..3865)
                     /locus_tag="IW671_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0899634.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
BASE COUNT         1219 a          843 c          716 g         1338 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgtacc tgcgtaaaaa acgcgatcgc
     3601 cgacagaaaa tttttgcact tcgtcaccta cagcaacaac tttcccgact ccatcaaaac
     3661 ctaagatacg taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat
     3721 taacagatac tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt
     3781 ctttttcatc aataaagctt tttggatcat cgatcggcaa cccttcataa aaaccgacta
     3841 ccttcattaa ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat
     3901 ccatacagtg ttcatttcaa tatatatagt atgctatttg ttccaccttt tttattcatc
     3961 ataattattc tcttacttaa agtaaactat ttgcactttt ttggttaaaa aagaggttgc
     4021 gacaagactt ttgtcacaac ctcttataaa cggtgttctc ctaccaactc ttcggtcaca
     4081 agtcaaaata ttcatcaaac atgagaagct gttttt
//
LOCUS       DACEKQ010000149         4074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEKQ010000149 DACEKQ010000000
VERSION     DACEKQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.158"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1051)
                     /locus_tag="IW671_002683"
     CDS             complement(77..1051)
                     /locus_tag="IW671_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0899635.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="IW671_002684"
     CDS             complement(1500..2915)
                     /locus_tag="IW671_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0899636.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="IW671_002685"
     CDS             complement(3137..3499)
                     /locus_tag="IW671_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899637.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3820)
                     /locus_tag="IW671_002686"
     CDS             complement(3503..3820)
                     /locus_tag="IW671_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899638.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
     gene            complement(3921..4061)
                     /locus_tag="IW671_002687"
     CDS             complement(3921..4061)
                     /locus_tag="IW671_002687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899639.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEKQ010000150         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEKQ010000150 DACEKQ010000000
VERSION     DACEKQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.159"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW671_002688"
     CDS             179..775
                     /locus_tag="IW671_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0899640.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW671_002689"
     CDS             899..2698
                     /locus_tag="IW671_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0899641.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW671_002690"
     CDS             complement(2976..3188)
                     /locus_tag="IW671_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899642.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACEKQ010000151         4034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEKQ010000151 DACEKQ010000000
VERSION     DACEKQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.160"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            246..689
                     /gene="rplM"
                     /locus_tag="IW671_002691"
     CDS             246..689
                     /gene="rplM"
                     /locus_tag="IW671_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0899643.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            705..1097
                     /gene="rpsI"
                     /locus_tag="IW671_002692"
     CDS             705..1097
                     /gene="rpsI"
                     /locus_tag="IW671_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0899644.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1186..2412)
                     /locus_tag="IW671_002693"
     CDS             complement(1186..2412)
                     /locus_tag="IW671_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0899645.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2484..2642)
                     /locus_tag="IW671_002694"
     CDS             complement(2484..2642)
                     /locus_tag="IW671_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899646.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3330..>4034
                     /locus_tag="IW671_002695"
     CDS             3330..>4034
                     /locus_tag="IW671_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899647.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1313 a          646 c          697 g         1378 t
ORIGIN      
        1 taaggggttg tgacaagact tttgtcacag cctctttttg tttttcattt tgacaggaaa
       61 cgaccctttt atataaaaga aagaaaagaa gtaaagtttc atataaattt ttcaatcaag
      121 cagaaaaacg ttgacattga ctagattagc gggtaatatg ttaaatggta ttgtttgccc
      181 cacaaagagc cccggaaact cgaagtgtat gtcaaacatc agaacttaaa ttggaggaaa
      241 ataacgtgcg tacaacatat atggccaaac caggcgaagt agaacgtaaa tggtatgtag
      301 tagacgcaac tgatgttcca ttgggacgtc tttcaacagt tgttgcatct gtactacgtg
      361 gtaaaaataa accaactttc acacctcatt tggatactgg tgatttcgtc atcgtcatca
      421 atgcagacaa agtgaaatta acaggtaaaa aagcgactga caaaatttat taccgtcaca
      481 gcaactaccc aggaggattg aaatcaatca ctgctggcga attgcgtgct aaaaattctc
      541 gtcgtttgat cgaaacttct gtaaaaggta tgcttcctaa aaacacttta ggccgcaaac
      601 aatttacaaa attgaacgta tacggtggag cagaacatcc acatgcagca caacaaccag
      661 aagttttaga catcacaaac ttaatctaat aggagggaat ttcattggca caagttcaat
      721 atatcggcac aggccgtcgt aaaaacgcag ttgctcgcgt acgtctagta ccaggaactg
      781 gcaaaattac tgtaaataaa aaagatgttg aagaatacat tccacatgct gacttgcgtg
      841 aagtaatcaa tcaacctttc gctgttactg aaacaaaagg cgcatatgac gtatttgtta
      901 acgtaaacgg cggaggatac gctggacaat caggagctat ccgtcatggt atcgctcgtg
      961 cattgctaga agttgatcct gacttccgtt tagctcttaa acgtgctggc ttacttactc
     1021 gtgacgcacg tatggtcgaa cgtaaaaaac caggtctcaa aaaggctcgt aaagcatctc
     1081 aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt tcaattgttt
     1141 gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat cacatttatt
     1201 caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg tatttacgta
     1261 tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat tcatatttgc
     1321 tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag ataaatgagc
     1381 caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat cagcgtgaac
     1441 agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat ctggtgtttg
     1501 attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc ttagtaatgc
     1561 cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa atttcttttt
     1621 ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt cgatatattt
     1681 ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa gcatgaaaag
     1741 agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg cttctatcca
     1801 agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc tttcttctaa
     1861 agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt taaattcaat
     1921 ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga taatttttat
     1981 atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag tatttgaatt
     2041 tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga atatatcttt
     2101 agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct gacctagttc
     2161 atatctagga agccattttg attcatagaa ttccttaaat tttatttttc cttttaattc
     2221 tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca ttgcattttc
     2281 ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg atgtaattcc
     2341 agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc catttgaaag
     2401 ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc gctattctta
     2461 gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa atctttgata
     2521 tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt atatatacga
     2581 atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg ttttttattc
     2641 aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt ttgaattgct
     2701 ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt acggaagtat
     2761 cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga aatctctaag
     2821 tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag cagggatcgt
     2881 tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct attcactatt
     2941 aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg agtttactga
     3001 ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa tgttttgatg
     3061 cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata cgccaataaa
     3121 gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt ttgaaagcta
     3181 cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa cagaaaaaaa
     3241 tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat atgatcgtga
     3301 taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt aatgtttgtt
     3361 ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt gctttaacag
     3421 gtattattgg tccaaatggt gctggaaaat ctacattgct taaagcaatg ttaaatatta
     3481 tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa ctggtgaaga
     3541 ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat
     3601 gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa aatgcagaat
     3661 ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa
     3721 taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa
     3781 atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt agtgaacgaa
     3841 taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta attgtgcatc
     3901 atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat cgaaatctaa
     3961 ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa gcctatggag
     4021 atactatatt tatt
//
LOCUS       DACEKQ010000152         4002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEKQ010000152 DACEKQ010000000
VERSION     DACEKQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.161"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..210)
                     /locus_tag="IW671_002696"
     CDS             complement(<1..210)
                     /locus_tag="IW671_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0899648.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IW671_002697"
     CDS             complement(309..623)
                     /locus_tag="IW671_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899649.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="IW671_002698"
     CDS             complement(691..1185)
                     /locus_tag="IW671_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899650.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1178..1492)
                     /locus_tag="IW671_002699"
     CDS             complement(1178..1492)
                     /locus_tag="IW671_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899651.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1708..3099)
                     /locus_tag="IW671_002700"
     CDS             complement(1708..3099)
                     /locus_tag="IW671_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0899652.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IW671_002701"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IW671_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0899653.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3699..3896)
                     /locus_tag="IW671_002702"
     CDS             complement(3699..3896)
                     /locus_tag="IW671_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899654.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
BASE COUNT         1169 a          750 c          535 g         1548 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattt gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ttcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 gccatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     3841 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa ttgcatgaca
     3901 atccccccct tttatttctt aataaattat cgaaaagtgg attaggatca ttaagtgtat
     3961 ctctgaataa ctcattgtct tcataagtga atcttcgtct tg
//
LOCUS       DACEKQ010000153         3933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEKQ010000153 DACEKQ010000000
VERSION     DACEKQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.162"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(605..1162)
                     /locus_tag="IW671_002703"
     CDS             complement(605..1162)
                     /locus_tag="IW671_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899655.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1341..1952)
                     /locus_tag="IW671_002704"
     CDS             complement(1341..1952)
                     /locus_tag="IW671_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899656.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2065..2322)
                     /locus_tag="IW671_002705"
     CDS             complement(2065..2322)
                     /locus_tag="IW671_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899657.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2919..3197)
                     /locus_tag="IW671_002706"
     CDS             complement(2919..3197)
                     /locus_tag="IW671_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899658.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3231..3482)
                     /locus_tag="IW671_002707"
     CDS             complement(3231..3482)
                     /locus_tag="IW671_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899659.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1190 a          806 c          524 g         1413 t
ORIGIN      
        1 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
       61 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
      121 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
      181 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
      241 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
      301 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
      361 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
      421 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
      481 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
      541 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
      601 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
      661 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
      721 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
      781 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
      841 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
      901 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
      961 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
     1021 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
     1081 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
     1141 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
     1201 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
     1261 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
     1321 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
     1381 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
     1441 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
     1501 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
     1561 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
     1621 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
     1681 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
     1741 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
     1801 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
     1861 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
     1921 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
     1981 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
     2041 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
     2101 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
     2161 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
     2221 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
     2281 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
     2341 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
     2401 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
     2461 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
     2521 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
     2581 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
     2641 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
     2701 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
     2761 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
     2821 acatttaaat tttttatgct gttcggtctc agaaatgctg tttttgctta tatcacaata
     2881 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
     2941 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
     3001 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
     3061 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
     3121 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
     3181 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
     3241 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
     3301 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
     3361 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
     3421 ataacaactc caaacattga atttttatac aatgaccatt tatttacagg cttttctttc
     3481 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
     3541 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
     3601 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
     3661 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
     3721 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg
     3781 taatactttt tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata
     3841 atatctaatc taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa
     3901 ataaatagaa actagaaaaa agcattacgc ctg
//
LOCUS       DACEKQ010000154         3816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEKQ010000154 DACEKQ010000000
VERSION     DACEKQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.163"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..1149
                     /locus_tag="IW671_002708"
     CDS             94..1149
                     /locus_tag="IW671_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ0899660.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            1169..1522
                     /locus_tag="IW671_002709"
     CDS             1169..1522
                     /locus_tag="IW671_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899661.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1704..2000)
                     /locus_tag="IW671_002710"
     CDS             complement(1704..2000)
                     /locus_tag="IW671_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899662.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2004..2822)
                     /locus_tag="IW671_002711"
     CDS             complement(2004..2822)
                     /locus_tag="IW671_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0899663.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYNLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3048..3269
                     /locus_tag="IW671_002712"
     CDS             3048..3269
                     /locus_tag="IW671_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ0899664.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            3271..3537
                     /locus_tag="IW671_002713"
     CDS             3271..3537
                     /locus_tag="IW671_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0899665.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
BASE COUNT         1311 a          669 c          628 g         1208 t
ORIGIN      
        1 ttctttctta taaacagttg caactattag taaataggtg taaactattt tcgtaccgca
       61 ataccataaa cacaaacaga aagaggtcat accatgaaag aaccattatt gttttccgtt
      121 gacttaggga ataaatcagt aaaagaaatg agtacacacg tttctccact ctcgcttccc
      181 tctcgtattt tgaacaaaaa tatcattaat tcaggacgtt tttctaatat aggttcctca
      241 ttaaaacgat cactaaacgt ttctgaatat agcctagtaa acactactga aagctattat
      301 tttgggaagg atatacacga gctaggaaaa gatgactacg tgatagaatc actaggtttt
      361 gggatcagca gatacaagca ggaaacctac caaaatatcc tatctttctc tttagctaac
      421 ctagcacgtt tagaagatca atctgatgac gttttaaccg ttaatctagt tttaggtctg
      481 ccttcatccg actataccga aacaatcgtc aaatacgtga tgcaacaagc cacacgacaa
      541 cattcagttg aagttgacgg taaagcttat aatataaacg tagataccgt ttgggtactg
      601 ccacagccga ttggaacact atataacctc ctccttgctg atgatggtag tgtacaagat
      661 gaatcattga tcagtcagaa gatcggtttt gttgatattg gtggtggcac gaaacttttt
      721 gatgttgtcc aaaattttga aattgacgaa aacaaccgtg aacaaaagga taccggtatt
      781 tttactttat ataatcgtat cgtaaatatg cttgaccttc cgatcagtcc taatgctttc
      841 caacttgaaa agattatccg agaaggcaaa gaaaaaggcg tatatctatt taagccaagc
      901 ggaaatgaaa gttttgatat ttcagaagta gttgaaagag aaataaaaac atacaccaat
      961 atggttgttg cagaagtttt agctacattc aaaaacttca caaccttaga taaagttgtc
     1021 tttactggtg gtggggcttc tattatcgac aaacaacttg taaaagataa aattccgcat
     1081 accttgtttg ttgaagacag tgaattttct aatgtaaaag gcttttacaa gtttggttta
     1141 actcaataaa acagcgaagg agatcaacat gaataaggga aaatcagagc gttatgtagt
     1201 tataatcaat agagaaaagg ttagcgagtt acctttagca gagtttcttg aatctgaata
     1261 tgaaacacta ggcgttaaat actctcagac gatcaaaaaa gctttgaatc tatatcggcg
     1321 tgtaaagaac aatgaagtta caattattga agcaggcaac gatagaaacg atagcaaagt
     1381 agcagaagta aagaataaac caactgatca taaccctatt caacaagagc atccttcaac
     1441 ggttacatcc gatcattcag caaagaaaga aaagccaatg ctaaaagggt tcaatgcacc
     1501 aagtaacaaa gacttacttt aaaaataaaa aggcgaggtt acaataccta gcctttttat
     1561 tatgtaaaaa tacaaacata aacatatacg tatacatgta tataaacatg tatacgtata
     1621 tatccataaa cacgtatacg aaatttagtt aagctatacc taaccgctta ttccaataaa
     1681 aaaagtgatc agctttaaac tgatcactca ccaaaaactt catcatacaa gttatctatc
     1741 atttcaccaa gtaaagctga atctgatcga attcctaact tttcgctgat tttagatagt
     1801 ttctttcttt gtgatggttg aagcgtgaat tgataatttt tcttcttttc agaactatca
     1861 ctttttagtt tagctaccaa agaacttgtt gtttctttag gtgtttgttt aggttcttca
     1921 actgcttcaa ccacttcagg aacttcttct acttcagctt cagcgaattt tttaaatcta
     1981 ttttctttca cttcaatcac tccttattct tgtgccgtat caattatttt gttgtatagc
     2041 gtaaacgtgc tttcaaagaa atccttattc ttacgataga tattcttatc cttagcgaac
     2101 tcagcaatat tcttaccttc taagctagtt gcattaagca attccttctc ttgtacatat
     2161 ccaagataat tagataacgt tggtaccaat tctaggaact cacgacttga attagtatta
     2221 tgtttgatcc tgctaccgat cgtagcgtat ttagtttcca tcagtgtttc accagtctta
     2281 atatcaatca actgttttgc tagtgcatca atatctttac caagcttcag taaagcttca
     2341 aaaccatctt tagatggatc agaaatgcct acgacaagat cagatacagc ccaagcattt
     2401 tgagctagta gacctgcatc attatgagta tcaatcagaa ttagattata cttctcttta
     2461 agctgttcat agttaacaac gatccatcta aacagtaggg ttctgttgtt aggcatatca
     2521 cgcatttgca tctcaacttg ttttaaatct tcacctgcta ctaaaatatc catgttctca
     2581 tttatagcta aaggttcagc tgatccatga agatataaat gattgattgt attctctctt
     2641 ttcgctttac tgaaagcatc aggaaagcgt tttgataggt tttgcgactt atcttcatca
     2701 atatacagca ccttcatacc taaattttcg acagcatatc cacctagatt gtagattgtt
     2761 gttgttttac ctgttccacc tttttcaatg tttccagtaa tgattgacaa cttcttttcc
     2821 atgattaaac ctcccttatt aaacttttgt aactccatta tacactataa acacgtttat
     2881 gtatatgttt atatacataa acgtgtttat atttatattt gcttttatat tctacaacat
     2941 aaacatatat atgtatatgt ttatgtttat gtatatgttc atatatatag ctattgcatc
     3001 tgtcatacaa tcgtagtaca atgtaattga aaaggagttg ataaactatg ccagtaataa
     3061 gtattcgtgt atctaaagaa gaaaaagatt ttcttgaaaa agttgctgaa ttcaacggag
     3121 ataacatttc tgaatttatg cgtagtaatt ccctgcaaac agcagaaacg ttagtagact
     3181 ttgccaccta caaggaacta atgaaagagc atgaggaaaa cgatcaaagt atttcccacg
     3241 aagatatgct aaaagagtta ggcttataag atgagttacc aagtaagata tgaacgtgaa
     3301 gctcaaaaaa cgctaaaaaa aatggataag tttcaggcaa aggttatcct aaactggatc
     3361 gagaaaaatc ttgtaggcac tgatgatcca agaaagcatg gtaaaggtct aacagctaat
     3421 aaatcaggct actggcgata tagggtaggt gcataccgta taatagctga tattagcgag
     3481 catgaagtga caattcttat attgtctatt ggtcacagaa gtgaagttta taagtaacag
     3541 acaagctgat aaggcttgtc tgtttttttg ttaacccaac aaaatcactg tttacgatcc
     3601 cactttacct aaaaaagaac aatatcctct tgttattata cacaccagtg tgtataatat
     3661 aagtgtaaac aagctaatta actttcaaaa ggagcgattt tttatgggat ggtatggtgc
     3721 ttattcatca gcaaataaag taatcgaaga aaagaaagga tacgccaaaa atggtttgga
     3781 aatcgtagat accaaagtaa cgaagaccta tggaat
//
LOCUS       DACEKQ010000155         3715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEKQ010000155 DACEKQ010000000
VERSION     DACEKQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.164"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            253..744
                     /gene="nrdR"
                     /locus_tag="IW671_002714"
     CDS             253..744
                     /gene="nrdR"
                     /locus_tag="IW671_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0899666.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            767..2140
                     /locus_tag="IW671_002715"
     CDS             767..2140
                     /locus_tag="IW671_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0899667.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2140..3066
                     /gene="dnaI"
                     /locus_tag="IW671_002716"
     CDS             2140..3066
                     /gene="dnaI"
                     /locus_tag="IW671_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0899668.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3195..3635
                     /locus_tag="IW671_002717"
     CDS             3195..3635
                     /locus_tag="IW671_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0899669.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         1319 a          628 c          814 g          954 t
ORIGIN      
        1 ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt aatcaaggtg
       61 atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc atgaaagtag
      121 aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt gatttgtttc
      181 gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat gcgatgcaag
      241 aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt aatcgacagc
      301 cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa ttgttcgttc
      361 cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa gaaaaacggg
      421 gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc agcagaaaaa
      481 agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa ccgcgtacgc
      541 agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat ggaagattta
      601 gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca gttcaaagat
      661 atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga aaataataaa
      721 gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg aaaatgcttg
      781 gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc tgagagaaga
      841 cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag ccttgagtat
      901 ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg aagggatgca
      961 tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg caagaaaaaa
     1021 gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc tgggacctcg
     1081 ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag actctgttct
     1141 ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta cccaattctt
     1201 tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat ttttagatgt
     1261 ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat cgctgaataa
     1321 atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat tcgattggca
     1381 gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc caatcatcaa
     1441 acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg gcgaactgat
     1501 cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc aaaaagccat
     1561 tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg tttctgaaaa
     1621 cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc tgataaaaga
     1681 agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa aaggcggata
     1741 tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag gtctttccag
     1801 tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg ctagcttaaa
     1861 tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg ccacagcaga
     1921 agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa aacaaaagaa
     1981 gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc ctgattgggt
     2041 caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga ttgaccgccg
     2101 ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt aggcaaagaa
     2161 ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat gatcgctgaa
     2221 gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga attgacacaa
     2281 gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa gcgtaaatat
     2341 caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac gttgaatttt
     2401 cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca aagacaagaa
     2461 gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc ggcttctttt
     2521 gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct agattttgta
     2581 gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt aggaagtttt
     2641 ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat tgctggattc
     2701 accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc aatcggaaaa
     2761 gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat gatcgatgac
     2821 attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt tatccttcaa
     2881 tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat ggatgaattg
     2941 gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc gaaacggata
     3001 atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa ccgacgcaat
     3061 ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca ggtcagcgaa
     3121 tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc agaaacttaa
     3181 acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt ctattttttc
     3241 ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg attgatgcgg
     3301 gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt gtcggaacga
     3361 ttgaaagtat tactcaagta gaagcagaag aagaaacgct tcagttgaaa ctcgtcgatg
     3421 tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac ggagtgatct
     3481 taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc ggtgacaaag
     3541 tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg caagttcctg
     3601 gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt caaaaataag
     3661 aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg aattt
//
LOCUS       DACEKQ010000156         3626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEKQ010000156 DACEKQ010000000
VERSION     DACEKQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.165"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            97..1086
                     /gene="galE"
                     /locus_tag="IW671_002718"
     CDS             97..1086
                     /gene="galE"
                     /locus_tag="IW671_002718"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0899670.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1098..2582
                     /gene="galT"
                     /locus_tag="IW671_002719"
     CDS             1098..2582
                     /gene="galT"
                     /locus_tag="IW671_002719"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0899671.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2606..3586
                     /locus_tag="IW671_002720"
     CDS             2606..3586
                     /locus_tag="IW671_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0899672.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1228 a          664 c          794 g          940 t
ORIGIN      
        1 ttttactttg tttgttttca ccataaaagt aaacttcgtt ttttaaatta tatagaatca
       61 acaaacacaa ttttactaat caaaggagtg ttttttatga caattttagt actaggcgga
      121 gccggttata tcggttcgca cgctgtagac cagctagttc aaaaggggta tcaagtagct
      181 gtggtagata atttgctcac aggtcacaaa caagcagtac atccagatgc tcatttttat
      241 gaaggagaca tccgggataa agaatttcta cgttccgtat ttgaaaagga accgattgaa
      301 ggagtaatcc attttgctgc aagctcttta gtaggagaat ctgtcgaaaa accattgatg
      361 tatttcaaca acaacgtata cggtatgcaa atcttacttg aagtcatgca cgagttcaac
      421 gtgaataaaa tcgtattctc ttctactgca gcaacttatg gagagccgaa agagtctcca
      481 atcacagaag atacaccagc taatcctaaa aatccgtatg gtgaaagtaa attgatgatg
      541 gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc gctatgtcgc tttgcgctac
      601 ttcaatgtag ccggagcaaa agcagatgca tctatcggag aagatcatac accagaaacg
      661 catcttgtcc caatcatttt acaagtagct cttggtcaaa gaaaagcctt ggctgtttat
      721 ggagacgatt atgatacacc agatggaaca tgtatcagag attatgttca agtagaagat
      781 ttgattgctg cacatatttt agctttggaa tacttgaaag aaggaaatga aagcaacttc
      841 ttcaatttag gcagcagcaa aggatactca gtcaaggaaa tgctagaagc agctagagaa
      901 gtgacaggaa aagaaattcc agcagaaata gcaccaagaa gagctggaga tcctagccga
      961 ttagtcgctt caagtgaaaa agcgagagag attctgggct ggaaacctga atacacagat
     1021 atcaaagcga ttatcaaaac agcatgggat tggcatgtca gccatccaaa tggttatgaa
     1081 gaatagggga atgagatatg tcaatcagtc aaacgatttg tgattttgcc acacttgcga
     1141 tcgaagctgg cggatggatg gaaatggacc gcttatattt acagaatcgt cttttaggga
     1201 tggtaggaga agatgctcta gaaagcattg aagtaagacc agttaaggaa gaatctgtag
     1261 acttattgga tcagcttgtc gctaaggcaa gagaaaatgg tgttctttca gaattgccag
     1321 cagaacaaga gatttttgaa gcgcaattaa tggattttct cacaccgcca ccttcagtcg
     1381 tcaatgcatt ctttgcacag cactattcga aaagcccaga ggaagcaact gagtatttct
     1441 ttaatttaag taagaaaaat gactacatca aaacaagagc catcgcaaaa aacatcaaat
     1501 ttccggcaga gtcggagtat ggtgaactgg aaatcacaat caatttatct aagccagaaa
     1561 aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa agtagactat cctacctgca
     1621 tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa ctatccagcc cgcaccaatc
     1681 accggattat tcgaatgaat ctagacggtg aaagctgggg attccaatat tccccttatg
     1741 cttattacaa tgaacattcg atcattttat cagaacaaca ccgcccgatg aaaatcacaa
     1801 aggaaacatt tgctagactg cttcggatta ctgaagtctt gcctcattac tttgttggtt
     1861 cgaatgctga cttaccaatt gttggaggat cgattctttc tcatgatcat tatcaggcag
     1921 gacgtcatga atttcctatg gcaaaagcac cgatagaaaa acttatcaaa ctagaagatt
     1981 atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc tgtgatccgt ttgcaatcaa
     2041 cagagaaaaa cttgcttatt gaagcagcta actcagtatt ggaaaaatgg aaagcatatt
     2101 cggatgaaac agtatctatc agagcttttt cagcagatgg tacaccacac catacgatta
     2161 cgccaattgc acgaagaagg gacaattact ttgagttgga ccttgtcctt cgagacaaca
     2221 atgtttcaga agaacatcct gatgggattt ttcatccaca taaagatgtc caacatatca
     2281 aaaaggagaa cattgggttg atcgaagtaa tggggcttgc tgttttacca cctcgtttga
     2341 aaccagaact agtagaagta gagcatttct tattaggaga agaaaaccag atggctgaat
     2401 atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc tgatttgacg aaagatacgg
     2461 tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc acgtgtactg gaagacgctg
     2521 gagtattcaa gcgagatgaa aaaggacagg cagcgtttga acgatttact gcgattttat
     2581 aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa gacattgcgc agttggcagg
     2641 agtttctcct gctactgttt caagagttct caactatgat cctgatcttt ccgttggctt
     2701 agccacaaag caaaaaattt ttgaagcagc agaacgctta aattatacga aacatcgtaa
     2761 aaatgcagca aatactaagc aaacgcttcg cctgatccag tggtacgacg atacggaaga
     2821 gcttgaagat ctttattatt tatctattcg cctaggaatc gaaaaaaagg cagaagaagc
     2881 aaacgttact ttattgaaag aaacgtggga ttccattagt gatcttccgg cagatggaac
     2941 gatcgcacta ggaaaattcg acgaagctca gttattagaa atgaaaggat cccaaaatgc
     3001 cttgcttttg gtagattctg acggcacttc tcaaggaatc gactctctag ttgtcgactt
     3061 taagtcaagt gttcaaaaag tcattgaaca ctttgtctcc cataaagcgc aacatatcgc
     3121 tatgcttgct ggaacagaat atacgaagaa aaaccatcag cggctaaaag atcccagaat
     3181 tatggcattt caacaagttt tggcagaaaa aggctacgac gaaggtccaa ttatcgaagc
     3241 agatttttct gtcgagtcag gatatcaagc agtgaagcag tatttaaaaa cgaatccgca
     3301 tgttcctgat gcgctttttg cagctaatga tgcattagcg attggcgcac tgaaagcgat
     3361 tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg atgggcttca atgatgtcag
     3421 tgtagcgaaa tatgtaacgc cctcgctttc tactgtaaaa gtttataccg aatggatggg
     3481 agagctagca gtggagacga ttcttcaatt aagtaattcc aaagcaccgg tttcaagaaa
     3541 gattattgtg gaaacagagt tgattattcg agaatcaacg aaataaaaga tggaaaagct
     3601 gtgggaaaat ggagtgatac cgcagc
//
LOCUS       DACEKQ010000157         3462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEKQ010000157 DACEKQ010000000
VERSION     DACEKQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.166"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW671_002721"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IW671_002721"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0899673.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW671_002722"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IW671_002722"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0899674.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW671_002723"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IW671_002723"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0899675.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW671_002724"
     CDS             complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IW671_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0899676.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT          991 a          757 c          594 g         1120 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aa
//
LOCUS       DACEKQ010000158         3404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEKQ010000158 DACEKQ010000000
VERSION     DACEKQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.167"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1073)
                     /locus_tag="IW671_002725"
     CDS             complement(75..1073)
                     /locus_tag="IW671_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0899677.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IW671_002726"
     CDS             complement(1148..1444)
                     /locus_tag="IW671_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0899678.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IW671_002727"
     CDS             complement(1437..1730)
                     /locus_tag="IW671_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899679.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IW671_002728"
     CDS             complement(1876..2427)
                     /locus_tag="IW671_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0899680.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IW671_002729"
     CDS             complement(2601..2900)
                     /locus_tag="IW671_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0899681.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="IW671_002730"
     CDS             complement(2927..3235)
                     /locus_tag="IW671_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0899682.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
BASE COUNT          990 a          665 c          464 g         1285 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atct
//
LOCUS       DACEKQ010000159         3389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEKQ010000159 DACEKQ010000000
VERSION     DACEKQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.168"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..120
                     /locus_tag="IW671_002731"
     CDS             <1..120
                     /locus_tag="IW671_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0899683.1"
                     /translation="GQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            366..962
                     /locus_tag="IW671_002732"
     CDS             366..962
                     /locus_tag="IW671_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0899684.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            975..1874
                     /locus_tag="IW671_002733"
     CDS             975..1874
                     /locus_tag="IW671_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0899685.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1877..2008
                     /gene="ssb"
                     /locus_tag="IW671_002734"
     CDS             1877..2008
                     /gene="ssb"
                     /locus_tag="IW671_002734"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0899686.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2030..2362
                     /gene="ssb"
                     /locus_tag="IW671_002735"
     CDS             2030..2362
                     /gene="ssb"
                     /locus_tag="IW671_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0899687.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2407..2640
                     /locus_tag="IW671_002736"
     CDS             2407..2640
                     /locus_tag="IW671_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899688.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2799..2921)
                     /locus_tag="IW671_002737"
     CDS             complement(2799..2921)
                     /locus_tag="IW671_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0899689.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3075..3335
                     /locus_tag="IW671_002738"
     CDS             3075..3335
                     /locus_tag="IW671_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899690.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLHTSHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT         1217 a          573 c          684 g          915 t
ORIGIN      
        1 ggtcagaagg gttcatttga agcaacgatt gttttaaaag aagacttcac tacaggcttt
       61 gaattttctg aaaagaaaaa aacgaacttc aagaaaagaa cacgccgcac tgcaaaataa
      121 tggagggtta atcttatgcg taataccaca cacaaacaac aagaaatcga aaatcaaaaa
      181 gccaaaattc gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaattg
      241 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
      301 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
      361 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
      421 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
      481 gggagctgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
      541 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
      601 tgtatgattt aacagatatg atccctagac agatcgatct gactgtacct tacggttatc
      661 acgtttctga aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat
      721 ggtatgaatt aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg
      781 atccagaaag aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca
      841 aagtaaaagc aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg
      901 agtatcgacg aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt
      961 aaaggagaag atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag
     1021 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
     1081 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttgattggct
     1141 caaagtatgg cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga
     1201 aagtaaccaa agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag
     1261 aagacattca atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag
     1321 gatttagctt acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     1381 tgactacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     1441 aggatcgaac gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa
     1501 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     1561 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     1621 ttacacgtcg aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac
     1681 tgaaaaatag tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     1741 ctgcgtcgat cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga
     1801 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     1861 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     1921 tgatttacgt tataccgcaa gtggtactgc agtagcgact tttaccctag ccgtcaaccg
     1981 aaatttcacg aatcaaaacg gcaattgaga agccgatttt attaactgcg tgatttggcg
     2041 caaaccagca gaaacaatgg cgacattagc taaaaaaggt agcttgattg gtgtagttgg
     2101 gcggattcaa acgcggactt acgacaacca gcaaggacaa cgtgtatatg tgactgaagt
     2161 tgtagcagat aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc
     2221 tgatcaatcg tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc
     2281 taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag
     2341 cgatgatgat ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaattagt
     2401 ctgacgatgg atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc
     2461 actgaaaatc ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat
     2521 cttgtctgtt cttatctttt aaaaagagca ggaaaggata attccttttg gcgattgact
     2581 aaagtcacag tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag
     2641 agttaacggg aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag
     2701 gagttgcgct aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag
     2761 ttatttggaa ataaccgctg acctgctaaa gtagacggtt atttcttttt gtcattttta
     2821 agcatattta caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt
     2881 tgaattgtct catatgcgga caaaaggctt actcctttca tagattttag cacttacatt
     2941 cataagcacc accaccttcc agaagtcgcc accgtcatta aactttactg tcttgatcat
     3001 tatagcatat ctaaaatcga ataaaaattc tcaattttag ctgtttttgc tataatttta
     3061 gcgaggtgat cgcgatggaa cattatactg gtttactcga taaaattcgg gtgatcaaga
     3121 cacagccatt gcttgtgcga tttacattac acacgagtca tgaaccaatc aattgtgtag
     3181 tggccaatat agaaatagcg gataaattgc tgattttgga agaaggcaag tatactattt
     3241 cagtgacagg tcacttcaat aaacgaaagc aactagtcat tgaatcaatg caaattagaa
     3301 accctgatca ttttacgcga tcgatgagca tataaaagaa gagctgcagt cctaaatgag
     3361 gattgtagct ctttttgttt tacatagaa
//
LOCUS       DACEKQ010000160         3378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEKQ010000160 DACEKQ010000000
VERSION     DACEKQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.169"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            254..580
                     /locus_tag="IW671_002739"
     CDS             254..580
                     /locus_tag="IW671_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899691.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IW671_002740"
     CDS             complement(697..1041)
                     /locus_tag="IW671_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899692.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IW671_002741"
     CDS             complement(1117..2364)
                     /locus_tag="IW671_002741"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0899693.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW671_002742"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW671_002742"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0899694.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          671 g         1054 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagttt
//
LOCUS       DACEKQ010000161         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEKQ010000161 DACEKQ010000000
VERSION     DACEKQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.170"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..588
                     /locus_tag="IW671_002743"
     CDS             37..588
                     /locus_tag="IW671_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0899695.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IW671_002744"
     CDS             1264..1530
                     /locus_tag="IW671_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899696.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IW671_002745"
     CDS             1588..2100
                     /locus_tag="IW671_002745"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0899697.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IW671_002746"
     CDS             2097..3134
                     /locus_tag="IW671_002746"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0899698.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3294..3376
                     /locus_tag="IW671_002747"
                     /pseudo
     CDS             3294..3376
                     /locus_tag="IW671_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1242 a          576 c          669 g          889 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac accgaccatc
     3301 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct acaacgatta
     3361 aaggcatgga agccat
//
LOCUS       DACEKQ010000162         3298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEKQ010000162 DACEKQ010000000
VERSION     DACEKQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.171"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(518..1132)
                     /locus_tag="IW671_002748"
     CDS             complement(518..1132)
                     /locus_tag="IW671_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0899699.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1738..2115)
                     /locus_tag="IW671_002749"
     CDS             complement(1738..2115)
                     /locus_tag="IW671_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899700.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2415..3296)
                     /gene="galU"
                     /locus_tag="IW671_002750"
     CDS             complement(2415..3296)
                     /gene="galU"
                     /locus_tag="IW671_002750"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0899701.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1014 a          671 c          501 g         1112 t
ORIGIN      
        1 ttacaatcat tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc
       61 cattatctat ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg
      121 acaattttgt tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta
      181 ctttgtaaag agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa
      241 tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg acactacata
      301 ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca
      361 aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg
      421 ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac
      481 attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga
      541 ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg
      601 ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg
      661 cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg
      721 atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt
      781 tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa
      841 aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg
      901 tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt
      961 gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac
     1021 caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta
     1081 tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct
     1141 cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa
     1201 aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat
     1261 tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa
     1321 tatctacata ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac
     1381 tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga
     1441 ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt
     1501 tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga
     1561 tttatatata tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc
     1621 ctttaaaaaa gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc
     1681 tctcatctca atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta
     1741 ccgtcttctt ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt
     1801 atgtctacct tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag
     1861 atacagtaca gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc
     1921 aaccatcaag gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc
     1981 tttttttctg accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa
     2041 tatccaattg cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc
     2101 gactaatttt cccatagtga acacacacct ttttttataa ttaaaaaatt cgccttttta
     2161 ttctactatg aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata
     2221 caacttaaaa aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct
     2281 ttttctatct ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag
     2341 ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg
     2401 ccacttattt ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca
     2461 cttaatgaag tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat
     2521 cctaacttat cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct
     2581 gtctggttaa gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct
     2641 tgagtttcta atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa
     2701 ttacttggtg cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc
     2761 ttttcgctat ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca
     2821 cgcatgacgg caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc
     2881 aatgggatat catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct
     2941 ataaatgctt ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg
     3001 aagtgaagat tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt
     3061 tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt
     3121 tcttttcccg ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata
     3181 aactggatcg ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc
     3241 ggcaaaaaac gtgttcctag cccagcggca ggaatgactg cttttttgac cttcactt
//
LOCUS       DACEKQ010000163         3269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEKQ010000163 DACEKQ010000000
VERSION     DACEKQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.173"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(368..1486)
                     /locus_tag="IW671_002751"
     CDS             complement(368..1486)
                     /locus_tag="IW671_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899702.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IW671_002752"
     CDS             1957..3264
                     /locus_tag="IW671_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0899703.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1023 a          678 c          589 g          979 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaa
//
LOCUS       DACEKQ010000164         3218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEKQ010000164 DACEKQ010000000
VERSION     DACEKQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.174"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW671_002753"
     CDS             50..1438
                     /locus_tag="IW671_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0899704.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="IW671_002754"
     CDS             1776..>3218
                     /locus_tag="IW671_002754"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0899705.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DACEKQ010000165         3215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEKQ010000165 DACEKQ010000000
VERSION     DACEKQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.175"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW671_002755"
     CDS             204..1172
                     /locus_tag="IW671_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0899706.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW671_002756"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW671_002756"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0899707.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW671_002757"
     CDS             2202..2810
                     /locus_tag="IW671_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0899708.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          955 a          615 c          778 g          867 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
     3181 ggaagattct agaaatattt catacttcca actat
//
LOCUS       DACEKQ010000166         3141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEKQ010000166 DACEKQ010000000
VERSION     DACEKQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.176"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(139..810)
                     /locus_tag="IW671_002758"
     CDS             complement(139..810)
                     /locus_tag="IW671_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0899709.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(892..2058)
                     /locus_tag="IW671_002759"
     CDS             complement(892..2058)
                     /locus_tag="IW671_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0899710.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(2064..2753)
                     /locus_tag="IW671_002760"
     CDS             complement(2064..2753)
                     /locus_tag="IW671_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0899711.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2880..3119)
                     /locus_tag="IW671_002761"
     CDS             complement(2880..3119)
                     /locus_tag="IW671_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899712.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
BASE COUNT          997 a          630 c          457 g         1057 t
ORIGIN      
        1 tgcttgatac ccctgaaagc agcttgcaat gattcctaac attccaacaa gcattccagc
       61 tttttttaca tatcgattta ttgccatttt tactctcctc atttcatatt tcaacgactc
      121 gcgttgatta aaaccttgtt attgtagctc tccctgtacc acgtatcttt cttttccttg
      181 tgacgtatag acacaagtaa acaacgttaa taagggtttc tctcctttct ttggcaaaga
      241 caagaaagag ttctcttcag gaacgccatc gattaatcta aagtacccag gtacgataaa
      301 tttacgcgtt accttgtaac gatagacgcc atcgttacta actaagtcca tcacctcccc
      361 taatgacaac ttttgaatat cagaaaataa aacacctggc atttccatgt tatgacctgc
      421 taggacatag tttcctttgc ccatttcagc atcttcataa aaataagtag ccgctccaag
      481 actcaaagtg tattgatttg cccctttaaa aatcggcaat gctaaaccaa gtgcaggaat
      541 ctgaatttgt gcaaatactt gtttattgac gcccatttcg attgcttctt tttgtatgga
      601 caacatttgc cccaaagaca tatcattatt gacgttcgtt gcatcctcct tataactctt
      661 tgcatgtgca ttgatttgat ctacgggcat ctttttttct atattttgct taaacacctc
      721 tccttcatgt ttttggtagc taacatagcc aaacacactg caaacgacga ttaaagcgac
      781 tcctaaaaaa ccaaataacc attttttcat ccatctgcct cctttcagac aaaacaaaaa
      841 acactttttc acaacgaaaa agtgttttct cttattacta ttctatttca tttatctggt
      901 ggttttatat tggtgtgtag ccattgctaa cattaagaac acaattggta cccaactgat
      961 tgtagctaat gtttcttcca tgaaagattg acacatgatc gctaaaaaag cccacaagaa
     1021 tgttagttgt ccatttgtca gctcacattt tcttacgagc aaccataaga aactaagcaa
     1081 aagaatcatc gatagagcaa aaccaatcaa tccatagtga ttgacgagtg cgtgatacgt
     1141 attatggtta cctagaatct gatttcctct atggaaaaag aaaggtttca tccctacaaa
     1201 tatttgtcct gttttttcgc ctaatgtttc atagaatttg cgccaaattt cttctcgtcc
     1261 tgtaaataaa tgaactttgt ctgacattgc agcaaaataa gaaatcggta aagctagaac
     1321 cgaaactgct gcatacatcc aaaaggacaa gtgtgtgttt cgaaagatct tcttaggtaa
     1381 aatttctagg ataacgaaga taacaagcgc gtataatgct gctttcgatt ggcaaaccca
     1441 aatcgttgcc ccagcaagaa tcaacgctgg taatgatagc aatcgcatgt accattttcc
     1501 aaatgaacta gcaaatcctg tgataagaag ggctaatgtc attaaagaac tacctatcgt
     1561 atttgtattg atccaaatat ctgataattt gttcgatgga ttccaaatac tttgtccttc
     1621 tggtactggt atctcggtaa atactcgata aaccgttacg agtaaaaaga catagaatac
     1681 tacaagatac aagctacgat caaatacagt aaattccgtc cattttacaa tcaaaataat
     1741 ggcaataatt gtcggaacga tcagatcacc atagaaagta tctcctcggt agcttctaaa
     1801 aaaagaaaag aaaaggtaaa aaagaatgcc tgctgcaatg aagaatagat cgtttctctt
     1861 taccttgtac agattcaaaa gaaacaaaca gcctacaaaa aacgtcacta actgtgcagc
     1921 attatcataa ataaataata tcgtaggagg atttaacccc accgtccata ttaaataaag
     1981 aatggatacc aacagtaaca gaaaaatggc actcatttta ttgatcaatt tttctacaag
     2041 ttcttgaatg tgactcactt cccttattta ttgatttttt ctacattgac taattcgcac
     2101 tggatcacat cgcacttttt catattattt gcttcaaagt ctaacagtgt gagtagtggt
     2161 tttttacctg cttttggcaa ggataaaaag gaattgtttt ccttagattg aactacctgg
     2221 ctgtaatcat aaatttttcc tcttttggag acttcatact gatacttgta atgagcatcg
     2281 attagatcaa tttcttctcc ttcggataaa tcaatcatat cggataataa aacgcttggt
     2341 gtatctacgt tataaccagc aagaatataa tttccatttc ccatttcagc atctggataa
     2401 taagtagaag ctactcctaa actaagcgtg tcgctattcg ctccttgaaa aacagggaga
     2461 ataaatcctg tagaagaaaa aacaagtttt ccagcaattt cacctttgag atccttttct
     2521 aatgcctgtt tttgattagt aatcagttgt ctcttagaaa tatccgttaa actggttaaa
     2581 ttatcttgat aggttttagc ctgttcctga atatggattg tttttgttac atcatctact
     2641 ttttcagcga ttttgtcctt ttgataacct tgataagcaa aataactaaa aaggagaaaa
     2701 acaatgatta cgcccacgta aggattaaat aaagttttat tgtttttatt catctatcta
     2761 ccttctatat attaattaat ttacctacaa ctaaccaaga taaaccacgt taaattttac
     2821 tattcttttt attcaataac aatgccacat cataattgat cattttcata tcataatcgt
     2881 caagaaactt ctaataatag ttggcaatta gaacttgttt tcacataagt tcgtctcaac
     2941 tgttcaaaat ttttcattga tcccaataga caggctttct cctttcctgt tatctgcata
     3001 tcattgatat attgcatgac agataatctt ttaaggtgtt tttgtttcat catttttagc
     3061 aacttttctt ccatttcttt tgacactttt ttccaattta cgctttcttt acaattcatg
     3121 agttttccta ccttccctgt t
//
LOCUS       DACEKQ010000167         3105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEKQ010000167 DACEKQ010000000
VERSION     DACEKQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.177"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1083
                     /locus_tag="IW671_002762"
     CDS             <1..1083
                     /locus_tag="IW671_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0899713.1"
                     /translation="TLFLILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGP
                     FSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPK
                     GTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGY
                     AAAGITEDSPYYTGCMSIITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSL
                     DKSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISG
                     LGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDW
                     RQAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            1099..1743
                     /locus_tag="IW671_002763"
     CDS             1099..1743
                     /locus_tag="IW671_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899714.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQANIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVVDFSYFTR"
     gene            1754..2560
                     /locus_tag="IW671_002764"
     CDS             1754..2560
                     /locus_tag="IW671_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899715.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            2582..2812
                     /locus_tag="IW671_002765"
     CDS             2582..2812
                     /locus_tag="IW671_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899716.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
BASE COUNT         1143 a          497 c          658 g          807 t
ORIGIN      
        1 accctttttc taatacttgc ggcgggttcg attgataatt cggattccga ttcaagtagt
       61 ggtggcgaag cttttacagg agagtattcg gaaggcttac cgatttataa agaaatcaaa
      121 ggaagaggcc ctttttctga tgagattgcg caatatgccg taggtgcagc agtgaaatat
      181 aaattattac caagtgtgat tctttcgcaa tacggttatg aatcagcgtt tggtacgtcg
      241 gcgtccgcaa gaaatgatct caactatttt gggattactt ggtttgatgg ttgtctgttt
      301 cctaaaggaa ctgcacgagg aattggtggc attgaagggg gctggtacat gaagtttccc
      361 aactcaaaag ctgcattctc ctattacggc tttatggtag ccacacaatc aaattttaat
      421 gcatgtgtag gaaataaaag ccctggcgca agcttattga tcttaggacg aggtggttat
      481 gccgctgcag gaattactga agatagtccg tattatacag gttgtatgtc aattatcact
      541 tctaataaat taaccgaata tgatgaattc gccatcaaac attggggtga aggtggcaat
      601 aacaatggca caattactgg tgaatggacg aatccattcc ctggcagcag tttagataaa
      661 agttccttca gcggtggtca attatttgga acgaatccag gcggtgaatt ccgtccgaac
      721 ggcttccatg atggattaga ttttggttca gttgatcatc caggaagtga gattcatgcg
      781 gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt gggcgcgtgt
      841 gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc aaatagtaca
      901 ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat tgggatacga
      961 gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc acaaggacat
     1021 gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag cagtaaaaaa
     1081 tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa tcgtcttaac agctgcaatt
     1141 agcgttagtt taatcggcaa tattgcgttg ttttggcagc tgaaagagga acaagcagca
     1201 aatacctata aaattgaaga agtggctaaa aacgcgtctg agaagctcaa aaaaaattcc
     1261 tctgaggcac aggcgaacat cgacaagtta aatcaaacca tcaatgagaa aaaccaagaa
     1321 atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac tgaagcacaa
     1381 agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag cagaaatgaa
     1441 ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt aagtcctgca
     1501 gacgataatc accaacatga tgattacgga agttactcct ttacattagg tgacgtgcaa
     1561 acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt tgttgattat
     1621 acaatcggca atccgcaatt caaagatgtc aaaccacaaa aaataaaagg tggattaact
     1681 gtcactgaaa aacaagtaga tggtcaatgg aaagtcgtag actttagtta tttcactcgt
     1741 taggaggaag aaaatgagta agaaaaaagg agaaagacaa cgtttcaaaa aacgaaaaag
     1801 ttatcttcaa tggctgggaa tctttatgat tctcggcttt ttgattcttg ctagtccgat
     1861 ttatacgaat cagaaggtag atataccaga tgaggtaagc acgaacgcct ttttaaacac
     1921 gaaagagctg acaatggttt caaaagaaaa aggagcgaac cagcaattag tttttgaatt
     1981 tgcgatcgac gatgaagatc aaagcttgtt aaaagaactg gctaatctta attatcgcgt
     2041 agaagtaaag acagctaaag gggattatca agcaatacga gccaaagtga tcaaggtgtc
     2101 tgatgattat tttgcagtga agctaacgca tgtaccagaa gaatatatcg ccatgcgttt
     2161 aaccattatt cctgaaaaaa ttgatccaaa agtggatatg caagaaccac aagatctgat
     2221 ttattacgtg cacgaagata aggttaaaga tcaagtgaaa gatgaagatt atgaacaaca
     2281 cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagctc aaaaacaaat
     2341 ggaatctttc caagcaacca ttgatttgaa tgaagaatta gtgaagaagc taaaagatca
     2401 attgccttat caagtcgcag atgatcagga aaatactgaa aacaagatta atagctacca
     2461 gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa gaagagatca ttcaaaaact
     2521 tcaagaaaaa attgatcgta tgacaaaaga aaagatttag aaggacaggt ggagtgaaaa
     2581 aatgaagcga acaaacttgg ctgacaaaga tactttcgct gagcaaaaag tattgattaa
     2641 agcctatctc aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt tatctaacac
     2701 aacaatatct gagttgaaga atggtcgaaa gaatttagat actttaaaat tcagatcgtt
     2761 tgaagctcta tatgtagctg ctaaaaaata ttttacggct gaaaggaaat aaaaacagga
     2821 ggattttatg tgaaaaaagt atatcaaaaa tgtttatgga tcagctatat caccgtaaca
     2881 caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga tttcaaccaa
     2941 acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt tttcatatga
     3001 caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta aatacacaaa
     3061 taaccttaat ttttaaagag cataaggaaa cgtgacccca ctttt
//
LOCUS       DACEKQ010000168         3069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEKQ010000168 DACEKQ010000000
VERSION     DACEKQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.178"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(334..696)
                     /locus_tag="IW671_002766"
     CDS             complement(334..696)
                     /locus_tag="IW671_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899717.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="IW671_002767"
     CDS             complement(770..1150)
                     /locus_tag="IW671_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0899718.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="IW671_002768"
     CDS             1232..2407
                     /locus_tag="IW671_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0899719.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="IW671_002769"
     CDS             complement(2484..3068)
                     /locus_tag="IW671_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0899720.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          958 a          587 c          504 g         1020 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccata
//
LOCUS       DACEKQ010000169         3057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEKQ010000169 DACEKQ010000000
VERSION     DACEKQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.179"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            718..2595
                     /gene="ltrA"
                     /locus_tag="IW671_002770"
     CDS             718..2595
                     /gene="ltrA"
                     /locus_tag="IW671_002770"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0899721.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2726..3057
                     /locus_tag="IW671_002771"
                     /pseudo
     CDS             2726..3057
                     /locus_tag="IW671_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         1163 a          480 c          657 g          757 t
ORIGIN      
        1 gtgaaaagct gtgggttgca agtagtctaa aaactattgt aataactctt ataacgagaa
       61 gtcaaatgag tgggtaacgc cacaaagggc ttccactgat actccgacat gcaacaagta
      121 gtttatgcag accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat accgtaaggt
      181 aaaagaaata gtgaatacca cgaaagttct ttagaggtaa agggtgtatc cgataagtcg
      241 ggtgtccaaa aaaacttaat atggtgagta tgtacaaacg tactgacgaa ctagcgaatg
      301 tactgtccga aaggcatagc tcctagaaat gggagagaca gtgagcaaaa aatcactcat
      361 tgtgctaact tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg cgttacaggc
      421 gtaggagaag ttagggggca aaagctagca tctaagttaa ggcatgtgga tagatataag
      481 ggaacagggg aagccatcaa catggagagt gcatactcga tgaagtggta gtaagaaata
      541 tgtgaatgac aaataactta ctttagtgat ggtgaaagta gagtcacgac tgatgaaggt
      601 tctgtaatgg aactggagga acaggctcga ttcgtctgta aaagtcatga aacccgttca
      661 accatgggat tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg ataaaccatg
      721 acaaaagaaa caagtccaaa agataggaag ttgcggcatg cagaatatta tggcatgacg
      781 gaaatcttcg ataagatata ctccaaaagt ttgaagggag ataatttcaa gaatctaatg
      841 cagattatca tttctgataa caacattgtc ctagcatatc gtaatatcaa gagaaatcac
      901 ggtagtgtga caagaggaga agatggagta acgattaaag atattgaaaa tctcaaccgt
      961 aatgaattcg tagaaattgt taggaagcga ttcgcacatt ataaccctcg gaaagtaagg
     1021 cgtgtagaaa ttcctaaata taatggtaaa ttaagaccgt tgggtattcc atcaatatgg
     1081 gatagaatcg cgcagcaatg catcttacaa gtattagaac caatatgtga agcaaaattc
     1141 aataatcaca gctatggttt ccgcccaaat agatcagcag aacacgcaat agcagattgt
     1201 atggcccgaa tgaatcaaag tcacatggaa tatgtggtag atgtagatat caaaggtttc
     1261 ttcgacgaag tcaatcatac taaactcatg agacaaatct ggtctatggg tatacgagac
     1321 aaacagcttc tcttaatcat taggaaaatg ttaagagcac ccgtagtact tccaaatggg
     1381 aagttgatat acccaacaaa aggaacacca caaggcggaa tacttagccc attacttgcg
     1441 aacatcaatc tcaacgaatt tgactggtgg atagcaaatc agtgggagga aaaagatgcc
     1501 aaagagctaa aacatcattt taggaaaaat ggagcttggg ataaaggtaa tatctatcac
     1561 aagctcagaa agagtacaac tctaaaagaa atatacctag tgagatacgc agatgatttt
     1621 aaaattttct gtagaaatta taatgaagcg aaaaaagttt tctatgcaac taaaatgtgg
     1681 ttacaggagc gtctaaagtt accaatatct gaagaaaagt caaaaatcac aaatctttca
     1741 aaagaagaaa gtgaattttt aggctttacg atgaaattag tgaccaaagg taacaaaaag
     1801 gttgttaata cgcatatagg tacaaaagca caaagcatga ttaaagtgaa actaaaaaaa
     1861 caaatcaaac gaattcaaaa aagtcctaat agcaaagata cctttatcta tattggagtc
     1921 tataacagta tggttattgg gattcataat tacttcaaaa ttgcgactca ttgtagtgta
     1981 gcaatgcgaa aaattgcaag agaactcaga ccaagttttg aaatacgact acgagaacaa
     2041 ggaatctcaa aagaaggaca atacggaaag tacgaaggat acaagcctta cctacggtca
     2101 ggacaaatac gatatataag agggtttcca atattaccag tagggtatat tcaacacaga
     2161 catccgatgt gtaaaaaaag aaatattaat aaatacaccg aagaaggtag ggaagccatt
     2221 cataagaagc aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag caatccagtg
     2281 cttagtaaga gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt catcgctcaa
     2341 aagggaagat gtgcagtaac aggtgaggag cttatcttat ctgaaatgca ttgtcatcat
     2401 attatccctt atcatgaaag caaaagtgat agctatgaaa acctagtgat tgttactgag
     2461 gaggttcatc gggtaataca cgctacacaa agtgaaacga ttgaggaact tctaaaatat
     2521 ctaaaattaa atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa agttggtaac
     2581 gaagaaatat cttaataaat atttgtagga cgggaaaaca gaatgtcttt tatagacgag
     2641 ggaacgccgt atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa agacggagat
     2701 aatatcaaac tcttacctat cagtatcgat taatccagat tttattgaaa cggtaaatta
     2761 tgtcacaaac tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca
     2821 atccccacaa gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa
     2881 agtctttgaa ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca
     2941 tatagaagga ttagttggta aatcaatgac gaactcagaa ctagaatcca ttcctcaaca
     3001 aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattt
//
LOCUS       DACEKQ010000170         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEKQ010000170 DACEKQ010000000
VERSION     DACEKQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.180"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW671_002772"
     CDS             complement(106..1053)
                     /locus_tag="IW671_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0899722.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW671_002773"
     CDS             1299..1763
                     /locus_tag="IW671_002773"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0899723.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW671_002774"
     CDS             complement(1825..2868)
                     /locus_tag="IW671_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0899724.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACEKQ010000171         2962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEKQ010000171 DACEKQ010000000
VERSION     DACEKQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.181"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..909
                     /locus_tag="IW671_002775"
     CDS             <1..909
                     /locus_tag="IW671_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0899725.1"
                     /translation="AVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSA
                     IIVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNAILFATTNAGA
                     EMYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGR
                     IVPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHL
                     VFDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGD
                     TDAEIIAKPSIAESKENKFVFSVGGV"
     gene            912..>2962
                     /locus_tag="IW671_002776"
     CDS             912..>2962
                     /locus_tag="IW671_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899726.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     A"
BASE COUNT          949 a          477 c          660 g          876 t
ORIGIN      
        1 gctgtagcaa gagggctata cggtgatgaa aataaagtcc ttttagtaaa tatgccagac
       61 tataaaacta aagatgatgc tatgcggttt agaaagctta tcggaacaca tgtccggcat
      121 gatccatcag caatcattgt ttgtgatgaa tatgaaaaag caggcgtaga aacacttgat
      181 gcctttttat ctattctaga tgaaggaaga atcagtttta ctgaaagagg atgggatgat
      241 cgagaagtta atgataccgt ctcactttca aatgcgattt tatttgcgac aacgaacgca
      301 ggggctgaga tgtacgattc gatgaaccga tacagctcac tatctcgtgt tgctaatcag
      361 gaaaattcaa atgagtacag cactgagctt aaaagagagt atcgaaaggt agcattagag
      421 attaaacaag ctctaattgg tgaaggtcta aaacccgaag tcatcaaccg atttggacgg
      481 attgttccat tttgggcttt atcggaacaa actcttttgg aaattgcaca caatgaaata
      541 ttgaaaagtg ctgaattggt aagaaaacac ttaaacgtag acttacgcct tccaccacag
      601 aaaaaacatt tctatggtca agaattttat gcagatgatc tctccatgca cttagttttt
      661 gatagagctt cacaagaaga ttctaaatca gggggtgcaa gaaatatctt gcatctggtc
      721 gaatcagaat ttgaagaagc aattactgaa gcaataatta cctatcctaa cgccaaaata
      781 tttgacgtta aattgagcaa agcaggaggg tttgaaggga aaggcgatac ggatgcagaa
      841 attatcgcta aaccttccat cgctgaaagt aaagaaaaca agtttgtgtt ttcggttgga
      901 ggggtgtagc tatgaaattt aaaaagtctt tacttcaaaa tcgtaaaatt aagcgatggg
      961 ttgtaagagg tttagttatc gtgggggcac ttgcagttac tttaggcgct ccatttggaa
     1021 tgaatgatcc aacgtctgat gatcaaggaa ataaacaaga aacagttgca aatacgaatg
     1081 tggcttatgc tgaaagtaaa ccggcagaaa aaggggtatg gacactatat gctcgtatcc
     1141 tgatgggaga taaagaggtc aatgatagtg ccactaaaac tggtcaaatg aatttacttg
     1201 gtcaaggagg tattagtgct gactttcctt attctgatat ttccaaacaa ggtaatgcaa
     1261 ttgccggtgg taacgcaggt acgcaattag catcaacctt agcgacttat aattactatg
     1321 ggtatatcga aacagtttca ggaaatgcga ttgcaagtca ggcatcctct tttattggtg
     1381 gtatcggtag aacgcttggc agttttatcg gatcaattag cttgatactt tctaatattg
     1441 ctgaatccat caatgaactc cttggaaaat ttttcatgag tatgaatata ttttcactta
     1501 tgggttgggg taaaggtgga tcggatgttt ctaatccggt agctaaggcg ttaactagct
     1561 tacttaataa tttgggatta aacaatcaat tatttaaagc gatagcaagc ttatctatgc
     1621 tcttttttac agcaatacta ggctatcaag gaatgaaact gttgtcgaaa gagggtgtta
     1681 actcaaaggg attatcacca gtacttgaaa aatggattgt tcgtattttt gtattgtttg
     1741 tttttctacc agttgcagga tcgttattgg gagtttatat caatggtatt cagtcagcaa
     1801 aagaagatgg aaaattacca acaaatgttg ttggtgatta catcattaat acaagacttt
     1861 gggcttctac tcaaaatctt gctccatcat ccgatactgg tgggaatgtg ccaaacggaa
     1921 aaccaacatc tgaaattaaa tttgttgaca ctgattatga accatctaaa aaatcagaaa
     1981 tgatcagaat gatcaatact agcagttacg ctagacaaaa taatgatcta gattccaatt
     2041 cagtcggcat ggcattgtta aaagaatggt caagtaatga tacgtttaat gtcaatactt
     2101 atgccggtga tgtgaatgta gctgaaaatg atgcttatat gggaagagcg cctgctgttt
     2161 ggacaggcta taatttaggg gatggaaaag aaggaaatgc aattaagaaa cctaccaatt
     2221 taaatggtta tatttggtca gctaatcaaa acgttacaga cgacaatcgc aagcctgatg
     2281 gaaaaggaac aacgaataac tttgatgcta ctcaaccttg gggtgttgct aacaatgcaa
     2341 cgtttagcac acagtcggta gcgttgatgc tccaatcagc actggataca tcaggggcta
     2401 agttttatgc gtataatatc gctccatcag gaattcaagg tagtgctaag aatatgtcaa
     2461 cagtaaagac agaatggcgt acagcttctt tagttggtca tggagctatg ggtaaattcg
     2521 gctcatggtt agcgatgaac gctgaatcaa tcgtattagc agtgctttat ctagcttgtg
     2581 cttgggcgat gtttaacgtt aatattgtaa aagccttcaa acgtgagttt gtgcgagttt
     2641 gggaagtagt cttaacaggg tctcttgccg caggagcagg ggttatggca atctcattag
     2701 gagtcgtaac cacatctctt atcggattac aattacctat ttttctgatc acctttgttc
     2761 gtgcatttgg agatacggct gtaggattca tccctaacac attacttggt gatggtgtag
     2821 caaatattgt acgatccgct atggtcttag gcgtaggata ccttctagtc tttaagaaag
     2881 gtgaaaaggt taataataat ttgattaata tgcttattgg attaccactt gaagtagcgt
     2941 ttggttttga cgaacgtgct aa
//
LOCUS       DACEKQ010000172         2893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEKQ010000172 DACEKQ010000000
VERSION     DACEKQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.182"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..950)
                     /locus_tag="IW671_002777"
     CDS             complement(<1..950)
                     /locus_tag="IW671_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0899727.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFV"
     gene            complement(1299..1625)
                     /locus_tag="IW671_002778"
     CDS             complement(1299..1625)
                     /locus_tag="IW671_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899728.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1612..2400)
                     /locus_tag="IW671_002779"
     CDS             complement(1612..2400)
                     /locus_tag="IW671_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0899729.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          862 a          470 c          395 g         1166 t
ORIGIN      
        1 acaaaccaat tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt
       61 ttttgttttt gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca
      121 taagattcca gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt
      181 gcattatgaa tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt
      241 atcaagttta atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg
      301 acgaagttat ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt
      361 ctattagtgt ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt
      421 gagtccttga tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca
      481 ttgatttctc gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg
      541 tcttcctctt ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac
      601 aataaattag gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt
      661 ttcagaagac ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat
      721 tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct
      781 acccagttat ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt
      841 gcatcatttg atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa
      901 tatttttcac gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct
      961 ctttctgtag gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct
     1021 atccactata tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt
     1081 tttttatctt tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag
     1141 gtttaagctc atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa
     1201 tattacaata tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa
     1261 atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt
     1321 gctagtttat atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct
     1381 ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac
     1441 attagaattg gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt
     1501 atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa
     1561 ttctttttat ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct
     1621 gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc
     1681 agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac
     1741 ggttattgtc ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc
     1801 ggttgctgca gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag
     1861 aactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact
     1921 taactttatt agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc
     1981 agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat
     2041 ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac
     2101 ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc
     2161 tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt
     2221 aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc
     2281 aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac
     2341 gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat
     2401 taaaatatcc cccattcatc ttatattata atataattat atcataatat tgtgatattg
     2461 caatatcgta tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata
     2521 tcggttcgga tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt
     2581 actttaaaat tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt
     2641 taaacattac taaactgtct atattgttac taataattcc cttcttatag tgcattttct
     2701 tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg
     2761 attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt
     2821 ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc
     2881 ttaaatcata aac
//
LOCUS       DACEKQ010000173         2656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEKQ010000173 DACEKQ010000000
VERSION     DACEKQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.183"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..362
                     /locus_tag="IW671_002780"
     CDS             <1..362
                     /locus_tag="IW671_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899730.1"
                     /translation="PEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDT
                     GRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAK
                     RKNEIYYEQQLSQKERV"
     gene            423..1892
                     /locus_tag="IW671_002781"
     CDS             423..1892
                     /locus_tag="IW671_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ0899731.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILISYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1903..>2656
                     /locus_tag="IW671_002782"
     CDS             1903..>2656
                     /locus_tag="IW671_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0899732.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQ"
BASE COUNT          989 a          441 c          570 g          656 t
ORIGIN      
        1 aaccagagat ccaaccagca gaagtgaagc aggagcataa aaatactgta gaaacggcag
       61 aacaaaagaa tattgtgcct gttggttcac tttggatcga caagaaagac gggaaagtaa
      121 tggaagaaga tacgggtcga acgttacatt tgaaggatgc ggcgtttatt gaagatccac
      181 agcataatca agtaatttta aaaggaaaaa gttatggaaa agagatccaa acggcgttac
      241 cttcagaaga atttgcgcta ggaaattcat tccgattttc acttgagaca gtgtcagaag
      301 tggcaaaacg aaaaaatgaa atctattatg aacaacaatt gtcacaaaaa gaacgtgtat
      361 aaaacaagta aaaaagacta tctgaaaaac ggatagtctt ttttcttttg ggaggtttta
      421 aaatgaccga gaaaaaaatg agtcttattg atcgatgtaa acagatcgat atagtggatt
      481 ttgctcgaaa caatggaatg gctgtagtca ataaaggacg agactatcgc ttagaagacc
      541 atgattcatt tgtttttgat cgaagaaaac aacgttttta ttggaacagt caaaatatta
      601 gtggcgacat tattgaatta gctaaacttt ttttcataga caaagagatc caagacccta
      661 agcaacaatt taaagcagcc actgatttta ttctaaagaa tgaagacaaa acagaacgag
      721 tggaaaatct tcattttgag acagaaaagt acaaagatca ccccgttgat tatcaaccac
      781 tcacagaaaa aggaaggaat tatctgaagg aagaacgaaa actaccagag tggctgattg
      841 attatgcaga aaaagaaggg ttgattgctg aactaaagcc gaaacatgaa cggcaaaatt
      901 ttttagttgg agacgaccgt ttagatcatg cggttgcttt tttatggaaa gatccacaga
      961 caggagagac agtaggggca agttatcaag gaacgatcgt cgattttaat cgtttcggta
     1021 aacggggaac gtataaacac attgacaaga atcctacgcc aaatcacgga tttaatttaa
     1081 aaataggcga tccgaagcat ttaaaattct ttgaaagtag tattgatctg ttaagttacg
     1141 ctgcattgaa tcgggagaaa ctacaagatg cctggttagt ttcgatggac gggttaaaac
     1201 atcatgtaat cagtcattac gttgaagagt cgatttctga attaagacga aaacaaacat
     1261 ttcctcaatc gattgaaata tgtgtggaca atgatcgtgc aggacatatt ttttatgaaa
     1321 aagaacagat gaagggaatc gttgatcctt ttaccaataa gaaaatccgt tgtgagcgag
     1381 gaattccaaa tgattggcaa gtacctaaag aatacaaagc tacttatgaa gcagtcgcaa
     1441 aagaaatgag tgttgaacct gaagcaatta tggccataca caaaactgaa actaatttac
     1501 agctgacaaa tcaattggtg tctgctcatg atgttcaatc tacgtttgga aagatgttgg
     1561 cgaagggcga accagtagaa acaattgatt tgaaagaagc atgtaccacg gtcgcgaagg
     1621 aactgaaagt atgtgaacgc gcagatggaa catacaactt tgatcgtttt tatagtagaa
     1681 aagccaacat aaaagacgtt aatgcaggaa tccttatttc ctataaagca gaacaatact
     1741 acaaaggcta taaaaagcat gaacatgaat ttgtacctga agtgaaaaag gattggaatg
     1801 atcaattgaa gcatgagatc cagcaacaag aaatcagaaa gcaaaaacgt gcgatgttgt
     1861 ttcaacaagg cagacaacaa gaaagggagt gaagtcgtca ctatgaaaac cgtcatttta
     1921 gcagaaaaac cgtcacaagc gaaggcttat gccgatagct tttctaaagc tacccgaaaa
     1981 gatgggtatt ttgagatcca agatcgattg tttacaggag aaactgtgat cacgtatggt
     2041 tttggtcatt tagtcgagtt agattcgccc gatatgtacg atgaaaaatg gaaacaatgg
     2101 gcattagaac atttgcctat tttccccaat caataccact atcatgttcc taaagataag
     2161 aaaaagcagt ttagtgtcgt caaacgacag ctgcagtcag ctgatacgat tgtgatcgcg
     2221 actgatagcg atcgtgaagg ggagctgatt gcctggtcaa tcattcaaca agcaggcgcc
     2281 aatcaaggaa aaacctttaa aagactttgg atcaactcct tagaaaaaga agccatttat
     2341 cagggattcc aacagttacg agacgcagaa gagacctatc caaaatttga agaagtacaa
     2401 gcacgtcaga tcgccgattg gttaattggg atgaacggta gcccactgta tagtttactc
     2461 ttgcagcaaa aaggcatacc aggaagtttt tctttaggta gagtccaaac cccaacactc
     2521 tatatgattt atcaattgca ggaaaagatt agaaacttcc aaaaagaacc atattttgaa
     2581 ggaaaagccc aagtaatcgc tcaaaatggc gcatttgatg cgaagcttga tccaaatgaa
     2641 acccaagcga cacaag
//
LOCUS       DACEKQ010000174         2647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEKQ010000174 DACEKQ010000000
VERSION     DACEKQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.184"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..362
                     /locus_tag="IW671_002783"
     CDS             <1..362
                     /locus_tag="IW671_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0899733.1"
                     /translation="PEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDT
                     GRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNQFRFSLETVSEVAK
                     RKNEIYYEQQMSQKERV"
     gene            423..1883
                     /locus_tag="IW671_002784"
     CDS             423..1883
                     /locus_tag="IW671_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0899734.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYIIKDENKVERVEN
                     LHFETQEYKHHPIDYRPLTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNY
                     IVQDDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTI
                     YEEVGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSE
                     VKKDWNDQLKHEIQQQAIRKQKRAMLFQQGRQQERE"
     gene            1894..>2647
                     /locus_tag="IW671_002785"
     CDS             1894..>2647
                     /locus_tag="IW671_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0899735.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDEKWKQWSLAHLPIFPNQYHYHVPKDKKKQFNVVKRQLQSADT
                     VVIATDSDREGELIAWSIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAHVITQNGAFDAKLDPNETQATQ"
BASE COUNT          947 a          451 c          586 g          663 t
ORIGIN      
        1 aaccagagat ccaaccagca gaagtgaagc aggagcataa aaatactgta gaaacggcag
       61 aacaaaagaa tattgtgcct gttggttcac tttggatcga caagaaagac gggaaagtcc
      121 tggaagaaga tacaggccga acgttacatt tgaaggatgc ggcgtttatt gaagatccgc
      181 agcataatca agtgattcta aaagggaaaa gttatgggaa agagatccaa acggctttac
      241 cttcagaaga atttgcgcta ggaaatcaat tccgattttc actagaaact gtatcagaag
      301 tggccaaacg aaaaaatgaa atctattatg aacaacaaat gtcacaaaaa gaacgagtat
      361 aaaacaagca agaaagacta tctgaaaaat ggatagtctt ttttcttttg ggaggtttga
      421 aaatgacaga gaaaaaaatg agtcttattg atcgatgcaa gcaaatggat attgtggatt
      481 ttgctcgaaa caatggttta gcggtagtca ataaaggacg agactatcgt ttagaagacc
      541 atgattcttt tgttttcgat cgaagaaaac agcgatttta ttggaacagt caaggtattg
      601 gtggggatat tattgaattg actcaattgt tttttattga cgattctatt caaaacccaa
      661 agcaacgttt taaagcagcg ctagattata ttataaagga cgaaaataag gtggaacggg
      721 tggaaaatct tcactttgaa acacaagaat ataagcacca cccaattgat tatcgaccat
      781 tgacagaaaa aggacgaagt tacttaaaag aacaacgaaa attccctgaa tggttgattg
      841 attacggaga acaacaaggg ttgatcctgg aaatgaagcc acgcaatgaa cgaaaaaatt
      901 atattgtgca agatgatcgc ttagactatg cgatcgtttt tccctggaaa gaccgtcata
      961 cagaccaaat attaggggca acttatcaag ggactatcgt agactatgaa cgttttggaa
     1021 aacggggtgt tgacaaacga atagatataa atcctacgcc taatcacggg tttaatgttc
     1081 ttcttggatc tcctaagaat ctgaaatttt ttgagagtcc cattgattta cttagttacg
     1141 ctgctttaaa tcgcgagacg atcaaagaca cgtggcttgt ttctatggat ggactgaagg
     1201 atgcagtagt ggggcactat gttcgagaag cggtgacaga attggcgccg aaagaagaat
     1261 ttccagaatc gattgagatt tgtgtggata atgatcgagc tggacacgta ttctgggata
     1321 aggtacaccg acttggtgtg tcgaatccat ttacaggaaa agcggtgtat tttgaaccga
     1381 atcttcccaa cgattggcaa gtaccgagag aatatcaaac aatctatgaa gaagtaggga
     1441 aagaaatgag agttgcgcct gaagcaatca tggctattca taaaatcgaa aacaacctaa
     1501 cggatactaa ccaaatcgta tcgttcggtg aaatccaggg agtatttgcc aaaaaacttg
     1561 cgccaaaaga gaaagcccaa tctattgttg taaaggaaaa atgtatagaa gcagctcagc
     1621 agctaaaatc gtgtgaaaaa tctgatggaa catacgattt tgatcgtttt tatcgtggaa
     1681 aagggaaaat taatgaacaa attcaatttt cattgaaggc caaacactat tttgaaggtt
     1741 ataaaaatca tgaacacgaa tttgtttctg aagtgaaaaa agattggaat gatcaattga
     1801 agcatgagat ccagcaacaa gcaatcagaa agcaaaaacg tgcgatgttg tttcaacaag
     1861 gcagacaaca agaaagggag tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaaa
     1921 ccgtcacaag cgaaggctta tgccgatagc ttttctaaag ctacccgaaa agacgggtat
     1981 tttgaaatcc aagatcggtt gtttccagga gaaactgtga tcacgtatgg ttttggtcat
     2041 ttagtcgagt tagattcgcc cgatatgtac gatgaaaaat ggaaacaatg gtcattagcg
     2101 catttgccta tttttccaaa tcaataccac tatcacgttc ctaaagataa gaaaaagcag
     2161 tttaatgtcg tcaaacgcca gctgcagtca gctgatacag ttgtgatcgc gactgatagc
     2221 gatcgtgaag gggagctgat tgcctggtca atcattcaac aagcaggcgc cgatcaagga
     2281 aaaaccttta aaagactttg gatcaactcc ttagaaaaag aagccattta tcagggattc
     2341 caacagttac gagacgcaga agagacctat ccaaaatttg aagaagcaca agcacgtcag
     2401 atcgccgatt ggttaattgg gatgaatggt agcccactgt atagtttact cttgcagcaa
     2461 aaaggcatac caggaagttt ttccttaggc agagtccaaa ccccaacgct ctacatgatt
     2521 tatcaattgc aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagct
     2581 catgtaatca ctcaaaatgg cgcatttgat gcgaagcttg atccgaatga aacccaagcg
     2641 acgcaag
//
LOCUS       DACEKQ010000175         2582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEKQ010000175 DACEKQ010000000
VERSION     DACEKQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.185"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..417
                     /locus_tag="IW671_002786"
     CDS             208..417
                     /locus_tag="IW671_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899736.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            467..1183
                     /locus_tag="IW671_002787"
     CDS             467..1183
                     /locus_tag="IW671_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0899737.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1085..1605)
                     /locus_tag="IW671_002788"
                     /pseudo
     CDS             complement(1085..1605)
                     /locus_tag="IW671_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1893..2546
                     /locus_tag="IW671_002789"
     CDS             1893..2546
                     /locus_tag="IW671_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0899738.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          836 a          392 c          558 g          796 t
ORIGIN      
        1 atgctcaaaa ggactgatag aatgatttac tatcagtcct agaaattata gcgccacatt
       61 tctttcacct ttgacttttc agacggcagt ttttatgctg atactgtaga gagagcagga
      121 cacagctttt gcttgttttg ggtaaggggg aaagaagatg accaaagcac ttatttttga
      181 ttttgatggg acgatgattg acagtgaatg gatgttttac gagagtattc atcactattt
      241 actgaagcct atttactgga gcagttcggc tataaaattc cttttgcaga ctatcaacaa
      301 ggtgttggga cggcagcaga actgtccttt tatgaaggga taagtcaagc ggttgggaga
      361 ccagtggatg ctgaacattt tgagcagcgg atcagcagca ctggaaaatc ggagtagtct
      421 caattctgaa ctgcgggagc tagactattt ttttgaagca catccgatga ttaaggaaaa
      481 aattgattcc tttgtgacga tcgatgatct tcaggtcggc aaaccagatc cagaaggtta
      541 tttgttgtgc ttgaaacggc tgagggttga acaggctgtt gtagttgagg atagtccagt
      601 cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa gcgttggatg cagatttttt
      661 cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc ggtgtattat atggcaaacg
      721 tcaattgtta gaacaaatgg agccagtcga attcggcggt gaaatgatag attttgtcaa
      781 tctttacgat agttcatgga aagagctgcc atggaaattt gaagctggta ctccgaacat
      841 tgcaggagca atcgattact tgaatgagat tggtatggag aatattcatc gttatgagca
      901 agaactggtt gcctacgttt tacctaaatt acaagcgatc ggcggtatca ccatttacgg
      961 accgcaagat cctgaacatc atactggcgt ggttgctttt aatttggatg gcatccatcc
     1021 ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct gtacgttcag gacaccattg
     1081 tgcacaacgg ttcatcgacg atttctgttt gactaattgg tcgtttaaac agtcgatgat
     1141 aatgccgttt gagcggctcc aatgtcgtgg aatctttgag taagagtttc caatatcgct
     1201 tgagccgacg gtacttcttc tgattttcag attacgtgat tgattttcgt acagttctat
     1261 gagaatcgcc tgcttcagtt cctttgttag ttgatgatgt tcatcaacta agtccgtttt
     1321 cgcagaaaag gtctgcccac aatcatagca tagaaaacgg gtacgtttta attctaaaat
     1381 agtcaacttt tctttgactt tcaacatctg agtacgtgtc gtataggaac cgttgcggat
     1441 aatacgaatc gatccacagt gttcacaatg ggaaggtgag tcatccaatc tccctttaat
     1501 aaactgcgct tgaacgcctt tatattcttt ttcaatcagc cattcttcat caaaaaataa
     1561 atgtttatct gttaatccga gtaattttct agtatgatta tccattgaga gactcctttg
     1621 gtaggatgac gttttggtcg actaaatttt accaaaagtg agtctctttt tgagttgaaa
     1681 cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc aacaacacaa aatattatag
     1741 aagccttttt tctaaaggat ttcaaggtgt ctgtttgcat ttattaaaaa aaagaaaaag
     1801 tattggcaac agaaggcgtc accaacacta gatattatag agccataata tgctgtataa
     1861 tatctagtgt ataggcttaa gtggaggtat atatgaaaat attaactgtt gaagatgata
     1921 atatgataag agagggaata agtgaatatc tttcagaatt tggatatact gttattcaag
     1981 ctaaagatgg aagagaagcc ttgtcaaaat ttaatagcga tataaatttg gttatcttag
     2041 acattcagat accttttata aatggtttag aagtgttgaa agaaattaga aagaaaagca
     2101 atttaccaat tctaatcttg actgcattta gtgatgaaga atataaaatt gatgcattta
     2161 ctaatttagt agacggatat gtagaaaagc cattttcatt gccggtctta aaagctagaa
     2221 tagattcttt aattaagaag aattatggac atttagagaa gtttgaatat aaggatctat
     2281 cggttaattt taatagctat acagctaaaa taaatggtga agaaatagat gtaaatgcaa
     2341 aagaactaga agtattaaaa tgtttattgg ataataatgg acaagtgtta acaagaatgc
     2401 aaattattga ttatgtatgg aaagatagcg aagaaactcc ttatgatcga gtaatagatg
     2461 tatatataaa agaacttaga aaaaaattac aattagactg tataactact ataagaaatg
     2521 taggatataa attggagaga aaatgaaaaa attaaagata tttccaaaaa tgttcataca
     2581 aa
//
LOCUS       DACEKQ010000176         2542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEKQ010000176 DACEKQ010000000
VERSION     DACEKQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.186"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            37..1377
                     /locus_tag="IW671_002790"
     CDS             37..1377
                     /locus_tag="IW671_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0899739.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1595..2038
                     /locus_tag="IW671_002791"
     CDS             1595..2038
                     /locus_tag="IW671_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0899740.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT          713 a          428 c          541 g          860 t
ORIGIN      
        1 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
       61 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
      121 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
      181 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
      241 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
      301 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
      361 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
      421 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
      481 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
      541 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
      601 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
      661 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
      721 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
      781 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
      841 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
      901 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
      961 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     1021 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     1081 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     1141 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     1201 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     1261 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     1321 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     1381 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     1441 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     1501 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     1561 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     1621 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     1681 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     1741 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     1801 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     1861 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     1921 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     1981 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     2041 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     2101 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
     2161 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
     2221 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
     2281 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
     2341 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
     2401 gcgtattaag aaaaattttg tgaaacggct ttccacattt ttgtataaat ttggcttaat
     2461 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
     2521 tcacaatccc tttttatgtt tt
//
LOCUS       DACEKQ010000177         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEKQ010000177 DACEKQ010000000
VERSION     DACEKQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.187"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1014..1304)
                     /locus_tag="IW671_002792"
     CDS             complement(1014..1304)
                     /locus_tag="IW671_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899741.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IW671_002793"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IW671_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEKQ010000178         2416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEKQ010000178 DACEKQ010000000
VERSION     DACEKQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.188"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..1147
                     /locus_tag="IW671_002794"
     CDS             2..1147
                     /locus_tag="IW671_002794"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0899742.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="IW671_002795"
     CDS             1318..1776
                     /locus_tag="IW671_002795"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0899743.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="IW671_002796"
     CDS             1935..2405
                     /locus_tag="IW671_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0899744.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          807 a          454 c          455 g          700 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaa
//
LOCUS       DACEKQ010000179         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEKQ010000179 DACEKQ010000000
VERSION     DACEKQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.189"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..2070
                     /locus_tag="IW671_002797"
     CDS             208..2070
                     /locus_tag="IW671_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0899745.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="IW671_002798"
     CDS             2070..>2401
                     /locus_tag="IW671_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0899746.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DACEKQ010000180         2258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEKQ010000180 DACEKQ010000000
VERSION     DACEKQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.190"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..914)
                     /locus_tag="IW671_002799"
     CDS             complement(<1..914)
                     /locus_tag="IW671_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0899747.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IW671_002800"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IW671_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0899748.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          760 a          390 c          427 g          681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttat
//
LOCUS       DACEKQ010000181         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEKQ010000181 DACEKQ010000000
VERSION     DACEKQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.192"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW671_002801"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW671_002801"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0899749.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          589 a          479 c          404 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACEKQ010000182         2181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEKQ010000182 DACEKQ010000000
VERSION     DACEKQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.193"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(210..1550)
                     /gene="glnA"
                     /locus_tag="IW671_002802"
     CDS             complement(210..1550)
                     /gene="glnA"
                     /locus_tag="IW671_002802"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0899750.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1578..1970)
                     /locus_tag="IW671_002803"
     CDS             complement(1578..1970)
                     /locus_tag="IW671_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0899751.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          620 a          484 c          368 g          709 t
ORIGIN      
        1 aacaagaaaa gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca
       61 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
      121 aaaatatgag gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt
      181 cacagcctct aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc
      241 cactcggaaa cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt
      301 gagaagatat gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata
      361 gcattatgga tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc
      421 attacataaa tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct
      481 agtcctgctt gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac
      541 cgcaattcca aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga
      601 ttacggccac tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa
      661 gaattgactg ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct
      721 aagaaatgat aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt
      781 ccttcatcat tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac
      841 ggtttcggca tgaaagtcgc gtgaagtcca tgcttgcgcg caatcgtttt gacaacgagc
      901 ttgaaggttt ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca
      961 tgctgtcctg gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat
     1021 tccaacacga tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa
     1081 ccacctttat cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag
     1141 aattctggtt caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat
     1201 gctcgtttca aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata
     1261 tcacaaatta aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg
     1321 gagagatctg gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa
     1381 gaaccgtcaa acatcatttt attttctaat actttgtcga tctggctcac tggaacttct
     1441 acatttttga tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct
     1501 tcctcaatga ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc
     1561 ttcctttttt aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga
     1621 cctgttttgc ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca
     1681 tgatctgtca acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat
     1741 acacgtttga tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac
     1801 atatcaatat cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact
     1861 aaatcttgtt cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact
     1921 gtaccaattg gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc
     1981 ctttcgtttt ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat
     2041 gttaggtttt ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca
     2101 aaaaaggtat ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta
     2161 tcctttatta gtgtttttat t
//
LOCUS       DACEKQ010000183         2169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEKQ010000183 DACEKQ010000000
VERSION     DACEKQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.194"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..112
                     /locus_tag="IW671_002804"
     CDS             <1..112
                     /locus_tag="IW671_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899752.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            326..1021
                     /gene="vanR"
                     /locus_tag="IW671_002805"
     CDS             326..1021
                     /gene="vanR"
                     /locus_tag="IW671_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0899753.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            999..2153
                     /locus_tag="IW671_002806"
     CDS             999..2153
                     /locus_tag="IW671_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0899754.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          731 a          400 c          461 g          577 t
ORIGIN      
        1 ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac
       61 taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg
      121 tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc
      181 tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta
      241 gtcatttatt atgtaaatgc ttataaattc ggccctataa tctgataaat tattaagggc
      301 aaacttatgt gaaagggtga taactatgag cgataaaata cttattgtgg atgatgaaca
      361 tgaaattgcc gatttggttg aattatactt aaaaaacgag aattatacgg ttttcaaata
      421 ctataccgcc aaagaagcat tggaatgtat agacaagtct gagattgacc ttgccatatt
      481 ggacatcatg cttcccggca caagcggcct tactatctgt caaaaaataa gggacaagca
      541 cacctatccg attatcatgc tgaccgggaa agatacagag gtagataaaa ttacagggtt
      601 aacaatcggc gcggatgatt atataacgaa gccctttcgc ccactggagt taattgctcg
      661 ggtaaaggcc cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa
      721 tgttatcgtc cactccggcc ttgtcattaa tgttaacacc catgagtgtt atctgaacga
      781 gaagcagtta tcccttactc ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa
      841 ggggaatgtg gttagctccg agctgctatt tcatgagata tggggcgacg aatatttcag
      901 caagagcaac aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac
      961 cattgataat ccgaaatata taaaaacggt atggggggtt ggttataaaa ttgaaaaata
     1021 aaaaaaacga ctattccaaa ctagaacgaa aactttacat gtatatcgtt gcaattgttg
     1081 tggtagcaat tgtattcgtg ttgtatattc gttcaatgat ccgagggaaa cttggggatt
     1141 ggatcttaag tattttggaa aacaaatatg acttaaatca cctggacgcg atgaaattat
     1201 atcaatattc catacggaac aatatagata tctttattta tgtggcgatt gtcattagta
     1261 ttcttattct atgtcgcgtc atgctttcaa aattcgcaaa atactttgac gagataaata
     1321 ccggcattga tgtacttatt cagaacgaag ataaacaaat tgagctttct gcggaaatgg
     1381 atgttatgga acaaaagctc aacacattaa aacggactct ggaaaagcga gagcaggatg
     1441 caaagctggc cgaacaaaga aaaaatgacg ttgttatgta cttggcgcac gatattaaaa
     1501 cgccccttac atccattatc ggttatttga gcctgcttga cgaggctcca gacatgccgg
     1561 tagatcaaaa ggcaaagtat gtgcatatca cgttggacaa agcgtatcga ctcgaacagc
     1621 taatcgacga gttttttgag attacacggt ataacctaca aacgataacg ctaacaaaaa
     1681 cgcacataga cctatactat atgctggtgc agatgaccga tgaattttat cctcagcttt
     1741 ccgcacatgg aaaacaggcg gttattcacg cccccgagga tctgaccgtg tccggcgacc
     1801 ctgataaact cgcgagagtc tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg
     1861 ataacagcat cattgacatt accgcgggcc tctccgggga tgtggtgtca atcgaattca
     1921 agaacactgg aagcatccca aaagataagc tagctgccat atttgaaaag ttctataggc
     1981 tggacaatgc tcgttcttcc gatacgggtg gcgcgggact tggattggcg attgcaaaag
     2041 aaattattgt tcagcatgga gggcagattt acgcggaaag caatgataac tatacgacgt
     2101 ttagggtaga gcttccagcg atgccagact tggttgataa aaggaggtcc taagagatgt
     2161 atataattt
//
LOCUS       DACEKQ010000184         2134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEKQ010000184 DACEKQ010000000
VERSION     DACEKQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.195"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            588..767
                     /locus_tag="IW671_002807"
     CDS             588..767
                     /locus_tag="IW671_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899755.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            826..1182
                     /locus_tag="IW671_002808"
     CDS             826..1182
                     /locus_tag="IW671_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899756.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          762 a          288 c          406 g          678 t
ORIGIN      
        1 aagctttaga actaacgtat gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa
       61 gtgaatggtt agattatttt gaaaatatca ataagtatgg cgcatattac accatgaaaa
      121 aggaagacaa cgaaaatgaa taaggtgatt tatcaactaa caacggattc catatcaacg
      181 atttgaaaga aaaaaaggtc aataatcatt tataccgaaa agtgagaatg tgtgaaacaa
      241 ggacaaaaaa agcctagtaa ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc
      301 gcccccgccg cttaggtggt gcgaaataaa cgggcaaagc ccgataactt aagagaaaga
      361 aggtgatcca tttgaacaag attattccct tattgttagt actgtttgtt ggcgaaatac
      421 ttatgcgaga tagtgcagct gtacagttaa acctcgctgt atttttggta atactggtgt
      481 tttgtttgat cttttggcgg tttgtattag tcctcgtgag tctactattc aagatgattg
      541 cagccatatt tttcataggg ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa
      601 taggaaataa acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac
      661 taaagcagaa gtatggcaaa agtgaatcta agattatgtg tattgcgtta gatctattga
      721 tcgcgcaaga aaaagcagga tttgatgtac cagcgctaaa aaagtgacgt cactttttaa
      781 tttgatctat ataagtgata tcacttttac caaggaggga attaaatgtt aaaaactaaa
      841 aagaattcgt acaaattaaa gcttagtata ctatctatta tttgtctttt aggcatgata
      901 attagtggtc atttgtatag agtggcatat acgcagttta ttgagtacaa ggttcactct
      961 ttaggattag ctggggatcg atacgttgaa tatgtccatt attcattaaa atgttcttca
     1021 tatattcttc tttggcttat tttttcattg tttgaattgc taaatttaat agcaatatct
     1081 tcatatcact tagataagct aaataccaaa tctagccgtt ataacgaagt gtttaaggaa
     1141 aatgtgaagt tagaaagtga cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt
     1201 taactaaaat tattttatta agaatgatgg aatataagga taaggttacc aacataacga
     1261 tcattacttc tctgatatgc tatggacttc tatcaatcag tattttgtct atagattatg
     1321 gagtaactat actgcaagcc ttagttttgg ctatgcctat gtatctatct ttgtttatca
     1381 tgcatgaaat atataagcta tttgttttat caaaaaatgt agaaatacgg gtaaatattt
     1441 tggtgaatag ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat ttattgaaag
     1501 gatgaaaaag taggatttga catactggta ttcagaaagt gccgtcactt ttaaaacaat
     1561 ggaaaaggtg gagataagat gaagaaaaaa caaactaaac tatcattagc agatatttaa
     1621 cacagttaac cgcaactgaa gatggtgttg ttgaatttga acaatctgaa attgcggtag
     1681 tagatgatcg ttaccttaaa atgccttact tctttgacca agcaaaggtc atttgccttt
     1741 gtcactacga tggggtacgt gattttaggc gttaaaaagt agtgattata tcaactttac
     1801 gagtaaatat aaatttagac atgaaactga attatccaat ggaaaatcat ggtttataat
     1861 ctagaaaaat tatagtaatg attggattat atatattctt tcgtattttc gtacaactgt
     1921 agcttgtcaa aattgagaaa tttaaccgat taggtacttt acatcgttat ttgggtaatt
     1981 tctaaaacat gaagcttacc aacgaataaa ctgagactat cttattaatt gatcaatata
     2041 agaaaatatc tttgaggaga tgatgtacta tgttttcgtc tatccacgat tggttaggtg
     2101 gtcttttcgg cggtggaggt aaaggttttt aata
//
LOCUS       DACEKQ010000185         2116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEKQ010000185 DACEKQ010000000
VERSION     DACEKQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.197"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW671_002809"
     CDS             complement(<1..424)
                     /locus_tag="IW671_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0899757.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IW671_002810"
     CDS             complement(533..1564)
                     /locus_tag="IW671_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0899758.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          664 a          339 c          361 g          752 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 ttcatataga aacaaa
//
LOCUS       DACEKQ010000186         2048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEKQ010000186 DACEKQ010000000
VERSION     DACEKQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.198"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..592
                     /locus_tag="IW671_002811"
     CDS             110..592
                     /locus_tag="IW671_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899759.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            789..1679
                     /gene="pstS"
                     /locus_tag="IW671_002812"
     CDS             789..1679
                     /gene="pstS"
                     /locus_tag="IW671_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0899760.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1966..>2048)
                     /locus_tag="IW671_002813"
     CDS             complement(1966..>2048)
                     /locus_tag="IW671_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0899761.1"
                     /translation="RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          672 a          352 c          419 g          605 t
ORIGIN      
        1 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
       61 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
      121 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
      181 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
      241 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
      301 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
      361 cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg
      421 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
      481 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
      541 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
      601 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
      661 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
      721 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
      781 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
      841 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
      901 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
      961 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1021 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     1081 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     1141 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     1201 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     1261 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     1321 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     1381 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     1441 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     1501 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     1561 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     1621 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     1681 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     1741 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     1801 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     1861 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     1921 aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     1981 cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc
     2041 aattcgtt
//
LOCUS       DACEKQ010000187         2026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEKQ010000187 DACEKQ010000000
VERSION     DACEKQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.199"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..672
                     /locus_tag="IW671_002814"
     CDS             <1..672
                     /locus_tag="IW671_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899762.1"
                     /translation="KLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGSIRLFKSV
                     VNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFPEEIIAQE
                     GKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIVDQQERFV
                     SEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQTKEILSE
                     IYALK"
     gene            690..>2026
                     /locus_tag="IW671_002815"
     CDS             690..>2026
                     /locus_tag="IW671_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899763.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPDKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYSEEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKE"
BASE COUNT          716 a          301 c          439 g          570 t
ORIGIN      
        1 aaattgagat ttatttttat tccaacgcta ttgatgataa ttggttttct tatactgatt
       61 agtcagtata tgtttattga tccatctagc ggatcactga tctcaggctc tattagactg
      121 tttaaatcag tagttaacac agcaagagta acgatgggga aaaagaaacc atttcgagga
      181 acggagattt tgaaagttag acaagatgga acgattatgt atcgagatgg tagtgtagca
      241 ctaatgttta atttggatgg tgctacctcg ttaaccgcct tcccagaaga aatcatagcg
      301 caagagggca aatcaattca ataccaaaat gcaagggatc gttccttggc tgaaatcaaa
      361 attacttcat cacaacctca gaatacggaa cgtcaaatac ggaacatgaa agtacaaaaa
      421 agaaatgcaa agagcaacaa agctgtgttt gacatagtag atcaacaaga gaggtttgta
      481 tctgaatata ttgatggggt caaaactacg gtgatccaat atcttatttt gaagtcgcaa
      541 tctcagatgg tattggatga aggcgtagaa cgtttagaaa attacacgag tagagggctt
      601 tactatagcg ttactcagtt gtcagctaaa caaaccaaag aaatattatc agaaatttat
      661 gcactgaaat aatagaggtg agaaatttaa tggaactaat gagtagatcg aagctactta
      721 atttcagaaa aaagaaacac aaaaaagagc aaaatgctga tagttctaaa gcggtgaata
      781 atacttatgt cagtgattta acatcattgg ttggttatca atttacacca tcttacatta
      841 ggacaggtaa tcggtgggga acgattgtta aattagtgaa tacgtatgga atgaacagaa
      901 attatgaatt tggatggatg gttaaagcta ttcctgaagt gaaggttgaa ggagtaaaag
      961 cttacttaat cgaagcggat aagccagttt cgcaaaaaga tcagcaagaa atcatgcacg
     1021 aaaatgtcgg tggatcaacg aacagttatg aaaataactt gaacgataaa aattcagatg
     1081 cagaaatcaa taaaactgtt atgcgaatca ttgatttgaa tacagcatcc ctgcttgaca
     1141 gtacggcaaa caaaattatt gattggaagc tagagatcct tttagtttcc gattctcctg
     1201 ataaaatcag cgaacagttg attaaactgc aacgcctata tgatgaaaag atgtatggat
     1261 taaaactcat gtcttttggt ggcgatcaac gtgatctgtt tgatagactt ttaagcgaac
     1321 cggtgggaag tgtttacgaa tacacatcta tgagttctga ttttgcagga aatgatcatg
     1381 ctattcgtaa aggcttagat gatgcagatg gatgggcgat cggtacgatg tctacaagct
     1441 acgcttcagg tgtagcgttt atggcaatgg atcgttcttt taagggtaaa gtcttagtag
     1501 catcccacaa agatgcaaca atcaaaggct atagcgaaga aatctcagca tcatcacttt
     1561 gggggcaatt gattgctaat aacgcaatgg caaatcataa acggacgttt cacatcgtat
     1621 tgaataaatt tgactacttt ggtgatccga caggggaaac atttgttgct aagccatctt
     1681 taaatagtgt gatagagaag attaatctag ctgaagaagg acttaattct ttagagcttt
     1741 ttggtgatcg aaaggatatt ggagatttat atagacgtaa tcttgataaa ctaactcaga
     1801 ttttctatct gttagcggac agagaattaa atcctgcaac aagagtattg ctgaagaaag
     1861 agttggggaa cttttatatt agtcagaagc tttgggatat taactatgaa gagcggtcga
     1921 aactgttagt aaacagcagt gatacaatgc cggttcttgg ggatttcatc agctatttaa
     1981 caaatgctga gacaaaagcg aaaaaatccg gtacagaaaa agaaat
//
LOCUS       DACEKQ010000188         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEKQ010000188 DACEKQ010000000
VERSION     DACEKQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.200"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..378
                     /locus_tag="IW671_002816"
     CDS             <1..378
                     /locus_tag="IW671_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0899764.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IW671_002817"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IW671_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0899765.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IW671_002818"
     CDS             1859..>2003
                     /locus_tag="IW671_002818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0899766.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACEKQ010000189         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEKQ010000189 DACEKQ010000000
VERSION     DACEKQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.201"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..367
                     /locus_tag="IW671_002819"
     CDS             <1..367
                     /locus_tag="IW671_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0899767.1"
                     /translation="KKQIVPKEIETWWRLEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="IW671_002820"
     CDS             567..770
                     /locus_tag="IW671_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899768.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
     gene            1334..>1932
                     /locus_tag="IW671_002821"
     CDS             1334..>1932
                     /locus_tag="IW671_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0899769.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVDDKVHPLKLSWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          648 a          289 c          443 g          552 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cggttagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaaaaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaata gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 ataactctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaacg agaaaaaaga gcaaaataaa
     1141 agccgagcag tgcgctaaca ctctcgacct ttacggaaac taatcgcact agctccgtaa
     1201 cttcttaatc attatacgaa tatttttaga tttcttcaag actagagatt ttttggtaat
     1261 gaatttttaa gagttacgtg aacttttgaa aattctttcc agaagtctct ttttctttga
     1321 ggaggaacaa aatttgagta aaaatggcat aatcgtaaat gagtgggaaa atttaacagc
     1381 cctatctaac aggcggttaa atgttgacga taaggtacac cccctaaaat taagttggtc
     1441 aatcgatcga attaccattg ttggaaaatt gaaagaaaat atttattatc acacaccaaa
     1501 tgatgtcttg attttaaatt ttgagcaatt gatgcaattg aatgagggga atgggtttgt
     1561 caaatcagtt ggtaaaaatg gctggcaaat tgtggataag catgatgaga atattgctta
     1621 cattgaaatt ttgaaatatc aagaagggca agggcgaatt gactttaacc caaataaaat
     1681 tagccaattt ttggcgggtt cgatgaagaa ctttatccat gatttatttt tagaaccaca
     1741 tttctcacgt gctgatattg cttgcgacat tatcgatgtg cctgatgatt ttattacgca
     1801 atatcgtgtg gttgaccctg tgtcttttaa gccaatttat ggccgttctg gcaaattaga
     1861 aacagcgtac tggggtagtc gtgccagcga gcgacaagtg cggatgtaca acaaaaagct
     1921 tgaacaggaa cg
//
LOCUS       DACEKQ010000190         1839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEKQ010000190 DACEKQ010000000
VERSION     DACEKQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.202"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..1082
                     /locus_tag="IW671_002822"
     CDS             24..1082
                     /locus_tag="IW671_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0899770.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1124..>1839
                     /locus_tag="IW671_002823"
     CDS             1124..>1839
                     /locus_tag="IW671_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0899771.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYR"
BASE COUNT          696 a          299 c          356 g          488 t
ORIGIN      
        1 tacgaaaggg ggcaggcaaa ctaatggaaa aagatgtcaa ttcagcatcc actaaagatc
       61 aatacaatat cattggagga ttacttggtg ataaggataa gagaacacgt catggattac
      121 gcattatgct tgcgacattt ctaggggtgt ttcttttatt aacaggcttg ggtagctaca
      181 tttatcaatt aggaaactat aattattatc ttgtcaacaa tacaacacca atcggctcat
      241 cattgacgtt tgctcgatca ggagcagaga tcactatctc agatgtttgg acagataaaa
      301 ctagagatgt aacggtggtc aaacttggtt actcaggaaa cgcaagaaaa ttgctatcta
      361 cacaaggtaa aaattataac ttgtatatgg caacaaaaga aaaagatcgt cctaagaagc
      421 ttgaaatgag ttatgggatg ttaagtaccg atggtgatgc tttcttattc ctaaaaggta
      481 aattagatga aagagcttat caagttttta ttgctaacca agtatcacta atttcaggtg
      541 attatacagc tgatgatgat accaaagtca atgtgaatga atcagattca gtcaccaagg
      601 cactttctca gtattctatg aatgatgttg ataatgaagg agtaatttct tttgggaaaa
      661 aagcaaaacc agttaatact cctgataata tcaatttcag aataaatcca tactcagata
      721 caactaacgt atatcaagga agcttcctta ccaaagatgg cgatattgat tactcaaaag
      781 ttgttgctgt gacaagtaag aataatgttg taaataagct agaagaccag ttaaatgctt
      841 caaatactaa catcaagaaa atcgaagcca caaaagctga gtatcttgaa cgtttacaac
      901 aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt cgaatggaga
      961 ccgaaattaa aacaatcaat gatacacaaa aattgattga tcagtacaaa aatgctgact
     1021 ttaacaaaga ttcttttgga gatatgaaga caacatttaa gtacctaacc ctgactgact
     1081 agaaacaaat tgttcttatt ttagaggaag tgaacacaaa cagatggaaa atagtaccga
     1141 gttctttaaa aatattcaaa ccgaattcaa tcatgctctc acactaatga aaccaccgaa
     1201 acataagtta gtggggcgtg atgacgaact ccatgagttg tcactgatct taaatagacc
     1261 ggaaacacca caagctatct tgttaggtga agcaggaaca ggtaaaacag cactggcaag
     1321 tgaatgggct aaaagacagc gacaagaggg gaagaaagta caagtatata aattatcaat
     1381 cggacaattg cgagttggag gagatgacgt cctccaaaca cgtttggaaa ctataattcc
     1441 aagattgaag gaatacgaac gaattttaaa atccgaggac aaagataatg aatttgtcct
     1501 ctttatagat gaatttcata aagttgtcag ttcatttggg agcggtaata aaattggggg
     1561 agattcgctg aaggaacctt tagcaaatga atcacttcag ttgataaccg ctacaactag
     1621 aaaagaatat gaaaactata tcaaacgaga tgatgcttta ggtagacgac tacaaccgtt
     1681 agaactcaac gaattaaaaa gtgatgtcgt tgtcgatata ctaagacaat ggttgaatac
     1741 atcatctgtc aaagatgatc ctgaagcttc cttgaaatta tccaaaaaga ttcctgatga
     1801 attgcttgaa gaaatcgtac aagcaaacaa gctttatcg
//
LOCUS       DACEKQ010000191         1750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEKQ010000191 DACEKQ010000000
VERSION     DACEKQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.203"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            516..1115
                     /locus_tag="IW671_002824"
     CDS             516..1115
                     /locus_tag="IW671_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0899772.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1179..>1750
                     /locus_tag="IW671_002825"
     CDS             1179..>1750
                     /locus_tag="IW671_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899773.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT          633 a          265 c          333 g          519 t
ORIGIN      
        1 agtcaaccga tggagaatta gaaacaatct acagtaggga agaaccattg acacaaaatt
       61 attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt cttataaatt
      121 agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat gactaaattc
      181 tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt atatagactg
      241 aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt tttaaagggg
      301 caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc caatgtgaca
      361 caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct aggcagaatt
      421 gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat tatatgttat
      481 aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa cctattcgag
      541 acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat ggccaacgaa
      601 atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat ttgcgtcaat
      661 tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa gatcgattag
      721 cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac aaattgcgaa
      781 aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag caagattttg
      841 acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc caaagtctgt
      901 ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc ccacattcca
      961 tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa atcattcagt
     1021 ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt acacaagaag
     1081 acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt taaacatgga
     1141 tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta tatattatag
     1201 gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt tcaaaatggg
     1261 ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat gaaaataatg
     1321 aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt agtttgcatg
     1381 agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat tatctcatag
     1441 gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat atggtttatg
     1501 attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata cgcaaagaaa
     1561 tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat gtactattct
     1621 ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa gaaaacatat
     1681 atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat tttgacaact
     1741 ttataggaga
//
LOCUS       DACEKQ010000192         1747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEKQ010000192 DACEKQ010000000
VERSION     DACEKQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.204"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            378..1628
                     /locus_tag="IW671_002826"
     CDS             378..1628
                     /locus_tag="IW671_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0899774.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          334 c          308 g          527 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtttgttt tttaacagac gacaatcaat tgtctatctt atctgaaatc tctgttgcca
      481 atgacatcga aggtgcttca ttcatacgag aaacaattct tgaattcaat aacagctacc
      541 actttgatca aattgtgatt ggcatggagt caacgtccat gtacagcttc catccttcca
      601 tgttttttca tgaagatgag aaactaaaga agctcaacac acttgtaacg attgaaaatc
      661 cttttcgggt gaaacaattt agtcgcatgt ttgatgagaa taaaactgat cgaaacgatg
      721 cattaagaat cgctgatttc ttgcgtattc aacgattcac aacttcccct atcaaagaag
      781 agaaatacat ggccttacag cgtttaacac gtactcgcta tcaacttatt aagcaattga
      841 ttcgtacaaa gcaacatttt cttgaaaatc taacgtacaa atgtaatacg cttgctcgag
      901 aaatgcgaga cgaatcgacg agtcttttta gtgcaaccct catttcactc atgacggaag
      961 atttcacatt ggatgaactt gctgagcttc ctttagaagc tttctgtgat ttacttcaag
     1021 aaaaaggaaa aggccgtttc aagcaaccag aaaaaatcgc taaagcaatt caacgtgcaa
     1081 ttaccatgag ttaccgccta ggtgctcttg cacaagaatc aattaatgtt gttttaagtg
     1141 ttctcgtacg agaaatacga gcacttgaaa aaaatatcaa agaattagat aaagccatcg
     1201 aacaagtaat tgttgtcatt ccagaatatc aatgcttaac cagtattcca ggcgttggta
     1261 aagtctatgc tgccggttta atcgctgaaa ttggtcaaat cgaaagattt gaagatcaga
     1321 caaaattggc aaaatacgct ggattaagtt ggaaagtgaa ccaatctgga aactatcagt
     1381 cgcaaaatac acctctgaca aaacaaggaa atcgatattt tagatactac ttagttgaag
     1441 ctgccaactc tgtaaaaaat tatcttcctg aatacaaagc tttttatcaa agtaaatata
     1501 aggaggttcc aaagcatcaa cacaaacgtg cactcgtcct aaccgcaaga aaatttacgc
     1561 gtctggtgga tacgctacta cgtaaacacc aactctatac gccaccaagg agtgtgatag
     1621 agaaataaca atccgttatt gacgcaaatc cttgaatcaa cccgaaaaaa atttgatttt
     1681 gatcgggtct agtttcgtgc gcttcaaaac atattactca ataaagaaaa tcttaaattt
     1741 catcttg
//
LOCUS       DACEKQ010000193         1743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEKQ010000193 DACEKQ010000000
VERSION     DACEKQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.205"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            628..1182
                     /locus_tag="IW671_002827"
     CDS             628..1182
                     /locus_tag="IW671_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0899775.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1436..>1743
                     /locus_tag="IW671_002828"
     CDS             1436..>1743
                     /locus_tag="IW671_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899776.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          693 a          177 c          290 g          583 t
ORIGIN      
        1 tactatattt taggtttcat attatataaa ttaataatat actaccattt gttggtagta
       61 taatttaaag gtaataagtt attcttttat tactatgttt gggttttata gcaaggaaag
      121 tttaataaga gtttggatct agcaaacaat attaatgagg tcattaaatc ttattatctt
      181 tagtgatata acgaaatatc tacacagaca aaattatgat cttgcgtaaa gttataaaat
      241 atcaataaaa atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt
      301 atttcacttt atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat
      361 ttaattatgt aaattagcta ttctaagaac ttctacggat aattgcccgt ctaaaattta
      421 aataagaggt tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa
      481 tattctgagt tactgaaatg atataaaatc ataaactaaa ccccttgatt ttcttcactt
      541 ttaacagagt atatatataa gttcatgatt atggtaaaat atatatataa tcatgaactc
      601 agaatatttt taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat
      661 gaagagatac aagattttca gttttggcta agaagaacaa gtaatccaga aagagatagt
      721 tttttatttt tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa
      781 gttggagaca tacgaggtaa aagtaagcct attattgttg aacataaaac aggtaagcga
      841 aaaccgattt ttatagataa tttgagagaa gagattttat tatatactga aggtaaagaa
      901 gaaaatgatt ggttatttcc aagtcggcaa caaggtcgac atataacaag ggatcgtgtc
      961 tatcagatat atgcagatat agcagaaaaa ttaggaagag atgatatagg aacacataca
     1021 ttaagaaaaa cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg
     1081 tttattttta atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat
     1141 gaaatagggg catcgttacg tggttttaaa ttgggaggct aattgagata ttaggatata
     1201 ttttttgtgt ataatgaaaa cttagaaggt gagattaatg aagataactg aattttcgga
     1261 agtatttaaa acgtttagta acatttacaa tagaatacca gagatagaac aaaatgttgt
     1321 agttgataat gattattata tagagaatga ttttaataaa ccaagaaaat atgacaacct
     1381 atacaatttt atacacaata gaacaacaat aaataacaac agtaacactc gagttttgat
     1441 taattcagca tattttcata ttaaatattt aaagaaatat gagtatgaag aagttgtatt
     1501 agattatggg ctaaatagac agtcattttt aatttatgca ataaataacg gaaatttgcc
     1561 aagcaataaa aagaacataa ttattgaaac atatgcctat ctggaggggg gatctaaaaa
     1621 actaattaag tcatgtaata tagagtatca attatcagta ggagaaatta aatgtgtttt
     1681 cgaagaagat ttagaacttg acttaaaaaa aatttttgcc agcattcatg attatatagg
     1741 ttt
//
LOCUS       DACEKQ010000194         1693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEKQ010000194 DACEKQ010000000
VERSION     DACEKQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.206"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(345..418)
                     /locus_tag="IW671_002829"
     tRNA            complement(345..418)
                     /locus_tag="IW671_002829"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Ile,seq:cat)
     gene            complement(607..1626)
                     /locus_tag="IW671_002830"
     CDS             complement(607..1626)
                     /locus_tag="IW671_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0899777.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          503 a          334 c          291 g          565 t
ORIGIN      
        1 taagcctttt ttctcacttt ttttcaaaaa tatggtatgc cttttaatag cttcaaatat
       61 aaaagagttt aaagcgtaac acacttatgg ggaagtggct tggggtgcgc tttaaactct
      121 tctttattat catctcacaa tttaacccaa atgtctttct atttaaaaat caaagtaaaa
      181 cttttcaaat atacagaaat ataactatgt gaaacatcct ttcattaatc cataaaagga
      241 tacataaaaa agccactcat ttgagtggca atgaagaaaa gctttagctt gtataatact
      301 cttcaaaaaa ttctaacaca gaacgattca aatggctacg ttaatgtacc ctgtaggact
      361 cgaacctacg accggacggt tatgagccgt ctgctctgac caactgagct aagggtactg
      421 gttgttgcca cataaagcca taaacaatca accagtagaa tgtgtggcaa caaacctgtt
      481 atcgcatagc ttggagtgtg actattatgg gtgatagtga agatatgcga taacatcact
      541 attttatcga atgattttta tagttgtcaa tatagttatg taatgctcct caacgaggag
      601 ctatttttat cgtttaggaa tatttaaata ccaacgtttg tcatggaaat cttgcgcacc
      661 gcctttagtg tttccctctg gatcattcgt tgcccgcatc atgacataga ctttcttatt
      721 agggaagtta cgcatgttaa aagatacatg atagccaaca tttccagaag tattataagc
      781 ttgatttaca tctgatctat aaattccatc agctcttact cgagctaatt ctttcccagt
      841 attgtaatcc ataatgaaga tatactcgta tttatagtta gcaatgtgcc atccagctac
      901 atgcaaattt gcattttcga tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc
      961 tgtcagtgta ggattagctg caccagctcg tgttggatca atgacaggct tgttttcaga
     1021 agttgttgga ttttcatcgg taaatccatg agctaaatca tatgctaatt tttctttact
     1081 tacgcccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaga tgttttgtgt
     1141 tacccataaa tgcgatttga ttcccggttg gttataagga gtgtccaacg ttaatggaat
     1201 accatatttc attgctgaat ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa
     1261 cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca ggactgttgg cattagcata
     1321 cgaaccagca ccgtactgta cataaccagg ttgaccgact tgataaacaa ttccgccgtc
     1381 tcccacaata taagcagtat aagcgctagt ccatgaacgt tgcatatact gcgcttcatt
     1441 gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa atatactgat tatttgctac
     1501 ttgtgagcta ccttcatttg cgcccaaatt aaattcattg ttgatagtat aggcaaatcc
     1561 gttaattggc aataaaaaaa gagccgttaa caggctcatc gcagtaatag taattttctt
     1621 tttcatatct atcctcctat ttatctgatg aaattttctt atacgttcga tttgatacac
     1681 ctaaaatact ccc
//
LOCUS       DACEKQ010000195         1678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEKQ010000195 DACEKQ010000000
VERSION     DACEKQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.207"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(365..1657)
                     /locus_tag="IW671_002831"
     CDS             complement(365..1657)
                     /locus_tag="IW671_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0899778.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          516 a          343 c          284 g          535 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatct
//
LOCUS       DACEKQ010000196         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEKQ010000196 DACEKQ010000000
VERSION     DACEKQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.208"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW671_002832"
     CDS             131..505
                     /locus_tag="IW671_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0899779.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW671_002833"
     CDS             complement(619..1248)
                     /locus_tag="IW671_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0899780.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDIA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW671_002834"
     tRNA            complement(1382..1454)
                     /locus_tag="IW671_002834"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          585 a          240 c          282 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca atgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEKQ010000197         1628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEKQ010000197 DACEKQ010000000
VERSION     DACEKQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.209"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(516..872)
                     /locus_tag="IW671_002835"
     CDS             complement(516..872)
                     /locus_tag="IW671_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0899781.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(886..1296)
                     /locus_tag="IW671_002836"
     CDS             complement(886..1296)
                     /locus_tag="IW671_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0899782.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          510 a          299 c          224 g          595 t
ORIGIN      
        1 ttccccgatt cgtttttggc tgccgtagca acttttatat catatcagag agacaagtaa
       61 ttgtcaactc ttttttaaaa gtttttttga tttcgttttt cgaaatctta cagcaactca
      121 tttatcataa cttatcattt gttatttgtc aaggactttt ttgaagtttt tttcgataga
      181 atcaagcttc acaagtggtt cttttttaac aactcagtta tcttatcatg tcatttctaa
      241 catgtcaaca actattttat atgtttttta aaatactatt ttattgcttt gctacttaaa
      301 acaacagaaa tgaatatatc atgtaaattt acacatgtca atatattttt aactctatat
      361 ccccacctag atttgaattt tgaccacata aattgagcat gtctctatag tagaaaaata
      421 aagtcaaaaa aagaacacat aacacgcttg atgctaagtg ttcgactgtt acaactgtat
      481 gaaatgctca gtgacttgat taaaacagtt aaagtctatt tatttaagat aagctgttcc
      541 atcaactgct cattgtatcc aataaataga acttcatcga taataatcaa aggacgtctg
      601 aggagattgg tatgttgtgt gcataaaaaa atcagttgtt tttttcctac acaggaaagg
      661 tcaatcgcaa tgttgtgaaa agcctcagac caagtcgcaa atagctgttc tgcttgcact
      721 tcttcaaaac tgcataattc ttctgcttct tctgtactta gatcgcgttc ttctaagtca
      781 acaaattgat aaggcaattg atggtgttct aaccattgtt tcgccttcag acttgttaca
      841 cagtgcgctg atccatagac tttcacttcc acgaaggctc cctccctatg aatttaagat
      901 gttattgttt tgtaaataga tcagatttct ggcaacacat cgaatttcac gagggacaaa
      961 tgaccggatc tcatcttcat taaatccgat tagtattctt ttttcatcaa tcaaaatagg
     1021 tcgtctcaat aatgaaggat attctttgaa taagacgatc atttgcttta aagttaattc
     1081 gttaaaatct atgcccagct tttcgtaaat tttcgaccga gtggaaataa tatcttgaac
     1141 tcctccttca gtcgctgcca agatcttttt gatctcactt tctgttaacg gttcggcaaa
     1201 aatattcttc tcttcaaatt caagttgatt cgcttgcaac catgctcttg cttttctaca
     1261 ggaagtacag ctaggcgaag tatatacttt tatcattttc tcactccttt ttaattataa
     1321 tccaattcta cagtaatttc ttttaaaata ccaattatta gctaataaaa tataaaaaaa
     1381 atcaaaataa ctgattattt acgtttgaat aatgagaatt tttctatata ttctctattt
     1441 tgataatagt attctcattt tcaaaataaa taagggaggt gtgacaaaat tcttgtcaca
     1501 cctccctttt ccgaataaac tgtgttccag aaatagcttc ttcgaaaata agctgaaatt
     1561 tcacaaaaat ttgagaagca attttcgtac attccttctt ctttcttgaa actgaccact
     1621 tctgtcac
//
LOCUS       DACEKQ010000198         1622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEKQ010000198 DACEKQ010000000
VERSION     DACEKQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.210"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            216..722
                     /locus_tag="IW671_002837"
     CDS             216..722
                     /locus_tag="IW671_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899783.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            709..1161
                     /locus_tag="IW671_002838"
     CDS             709..1161
                     /locus_tag="IW671_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899784.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            1154..1552
                     /locus_tag="IW671_002839"
     CDS             1154..1552
                     /locus_tag="IW671_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899785.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          644 a          190 c          270 g          518 t
ORIGIN      
        1 cgtaaactag tttggtaata caattgtaat ttattttcag tatttaaaca tttctacctt
       61 attacattat gttaactaaa ttgcttataa aataagctaa tttttattga caagcaggat
      121 gtactatagt taaacttaaa ataagataca aatatatata atatgcttat acatatagta
      181 atatttacta atattgaaac aggtggttta gtgagatgga cattaagtgg ttggttcaac
      241 aaaatgatag caatttagaa ttggcgatta aatatctcga agaaactatc tttgaagatg
      301 aacatcttac agataatttt ttacaagtat taaaatatct tgaaatttac tctgttaaaa
      361 aaaataaatt aattggtgaa aatgattctc caataaaaac acctattgag ctaagcttaa
      421 gaaatcggat gggaatactc caaagatcgg aaatagtaaa agaacttttt tatcataaat
      481 ttagttatga aataagacta gatgatactt atgagcatta tagaattgtt tttttcgtat
      541 acaattctat agaagatgct actgcgacta ctgcattaac attcggtttt acgaaaaatg
      601 gaacaatcaa ttcggataaa acccgtcaag ctgctacaga aagtgatgat atatgtaaga
      661 aagtatgcaa tggggaagag aattactgga taggggagga aaagttgaat gaaatctatt
      721 aatgatttat taaaaaaaca tcctgattta tataaaaaat taaattctga acaaagaaac
      781 ctgtccaaac aattacttaa aataagatta aaaaaacaaa aaaacgtgac agaattctca
      841 aaaatgttag gaataagtga aaaaaaatat cttgaatttg agttcggaga tatgcatatt
      901 cctgttgcag agtacaataa acttttgaaa caagttgaaa atttgtccta taaagattca
      961 atgaatttca ctattaaggt tgctggcggc cctattaaat atgaccgtat tgaaaataaa
     1021 ccttatattc ggaataaaag ttactcagta tttaaaagta gaaagagaaa agaagtatcg
     1081 tcaaatatgg tctatgctag tgttaattac caagatacga gccgctatat tgtagaaaaa
     1141 gaggtatatg tacatggata aaggaaatag tgttactttt gcacttgaaa atattagttt
     1201 gaataaaatt cattttgaat taactgataa ttttagttat ggagatttag agtttaatat
     1261 tgaacttgga tataaagaag acgaagcaga gtatctagaa aaaaaacttg tttctttacg
     1321 agctcacatt gtagggggga tggaaggagc accggtagat attttactct tttcagttat
     1381 tcgtgtttct acaaatgaac agccatataa aaaattagta gaagaatcta tgggagagct
     1441 agctaaacct atcctgaata aagcttctaa tttgatttct aatttatata gcgaagtagg
     1501 agttatgcct acagttattg atttatatca gctatatagt caacaaaaat aaaatacaag
     1561 taatagagtt atctatttaa atttgcaaga caaagacctc aaccagtcat ccttggttga
     1621 gg
//
LOCUS       DACEKQ010000199         1612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEKQ010000199 DACEKQ010000000
VERSION     DACEKQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.211"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            97..1161
                     /locus_tag="IW671_002840"
     CDS             97..1161
                     /locus_tag="IW671_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0899786.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKGELI"
     gene            1158..>1612
                     /locus_tag="IW671_002841"
     CDS             1158..>1612
                     /locus_tag="IW671_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899787.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSI"
BASE COUNT          542 a          191 c          259 g          620 t
ORIGIN      
        1 attccatcta tttataattt tggatatttg ctgattactt tgatagtaat ttttcctgca
       61 gacttttcac aaagtgaaga ggaaaaaaag aaaaaaatgg tgtattttaa taatgttttc
      121 attatcataa tttttttagc tgcgctaata tctatctatc aatttatttt tttgatttct
      181 tatcatgtcc caacaggaac gccaggatta cttgcaagac aaggttttat tgaacatcgg
      241 ttatttggtg tctatacaag tccaaatgtt ggagctatgt ttggatatac ttctattatt
      301 ttaagtttaa ttagtgtatt aatttcaaaa ataacaaaaa cgattcgtgt tttttatatg
      361 atgaatttta tcgtacaaat attttatatt acactttctt cgtctagagg tgcccaggta
      421 gtaatttgtt cttttttagt attatttctt ttgttgtttg ccaattcaaa atttagaaag
      481 agcattagag aagtactacc tgtaaaaatc aaatggatgg taagcatatt attagttcta
      541 ttggtatatt tttcttttgg tactaattat gctaaggatt ttttagcaac aattcctgta
      601 tcgattgata gaaaagtaga agttatagag tcaaatgaat cagataaaac tccacttcca
      661 gaaaaagaag taatattaca gcattctagt gataactccg aaatttcagc tggtcgtttt
      721 acgatttgga aagctgcgct taaagttata agaccaactt tgttatttgg ttatggagag
      781 accgatttct atgggaatag cgatgcaaaa tttttgaaaa aagtagatct tgatactaca
      841 gatgtgaatg aactaaaaag agcacatgga aacatgcata atggatttct tcaagtactt
      901 gtatcaagtg gtataattgc ttttatatgt atctatgctt tctatgtgtt aaatatcttt
      961 agtttactca aaacattttt tagaagtaaa gtgaattatg cgtttcttgg tattgtttta
     1021 atttttatac tatctatttt tatagatgaa atggtggagg ctcatgtact tttcaataag
     1081 cgcgatgtca tttcaattgt tttctggtat tatttgggaa gcatttctta cttgtattta
     1141 aaaaaaggag aattaatatg aagacagctt tcattgcttg gactccttta catttgatta
     1201 atatattaaa tgtttatcaa aatttttacc gagaatcaaa aggagattta tttatttatt
     1261 ctgaatttga aaacgctaga agatatagtg agcgtataaa agaattaggg ctatttaatg
     1321 atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt taaacgtaaa attaacttgt
     1381 taactaacca taatcaattt ttctcggatg atttatccgt ttatgatgaa atttttatac
     1441 agggggaaaa ttatttcaca aaaatacttt tctccaatgt ttacaaaaaa aatccagatg
     1501 taaaactaaa ttatatagaa gatggtttgg gtacttattt gaagaagagt atattttcaa
     1561 caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa aaatagcatc tt
//
LOCUS       DACEKQ010000200         1577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEKQ010000200 DACEKQ010000000
VERSION     DACEKQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.212"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            535..1554
                     /locus_tag="IW671_002842"
     CDS             535..1554
                     /locus_tag="IW671_002842"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0899788.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          541 a          257 c          332 g          447 t
ORIGIN      
        1 attttttgca ttatctacag ttcaacttat aatctgttta atgggacatg tgggtgccaa
       61 ccattttttg ttatgcaata gtactattta gttgttgcat ggatccgtag taaaagtcca
      121 agtacaacga gttgcgccag tttacatagc gttttgtatc cagaatgttg actaaaacaa
      181 aagttcctat gtcaaccgtc aacagagaca atctgtccgt tgcaggacct gacagattta
      241 gaaatgttca atctgtctct tatgtatttt aaatcaatac tcactaggag aagtttccta
      301 acctcctaat agtaatgctg gtagtaacat tgtcaaaact gcttcctctt aggaacaagc
      361 aaagcataac gagtgtaaaa ataaaatgta gactattgta acatatcatt gattatttca
      421 agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt
      481 taagaaatgt atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa
      541 caaaaaatcg cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca
      601 gaaaacgggc atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat
      661 aagtatcata caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga
      721 tataaagaat tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca
      781 aaagcgattc gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt
      841 atacatgcaa gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct
      901 gaagaaattc cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct
      961 gaggaagtgg cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca
     1021 gcagcctatg tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat
     1081 gtgattggcg tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct
     1141 atccatggat taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca
     1201 gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt
     1261 ggtgtggggg acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt
     1321 aacttgttag gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt
     1381 gagggtgtgg caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg
     1441 ccaattactc aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca
     1501 aaagaaatca tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat
     1561 gatcaatagt taatcgt
//
LOCUS       DACEKQ010000201         1524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEKQ010000201 DACEKQ010000000
VERSION     DACEKQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.213"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..883)
                     /locus_tag="IW671_002843"
     CDS             complement(<1..883)
                     /locus_tag="IW671_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0899789.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDA"
     gene            complement(883..>1524)
                     /locus_tag="IW671_002844"
     CDS             complement(883..>1524)
                     /locus_tag="IW671_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899790.1"
                     /translation="EYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEK
                     QRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSP
                     QRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKEYE
                     QQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT          384 a          303 c          266 g          571 t
ORIGIN      
        1 tggcatcata ataaatcgta ttcgtgaaag tattggttaa ctgtgtcgct gtgtctatgt
       61 ctcctcgctt ccagtatttc acgatgagtg ccaatggatt atcactcatt gattgcagtg
      121 gatcggtgat attaaatgca tacacatcat accctctagc ttctagttca tctttactag
      181 caacatataa ttctgttttt ggatcattaa caaccattga actttgtctt tctgcacgag
      241 acaaattgtc aatggttgct aaaacttcta tctgtccttt ccctgaacgg gaagtaccaa
      301 caatacaagt atttactgta tccgtctgaa tataccagta ctttttataa tgagtaattg
      361 gtattccgcc ataaccagga aaacctccct ttgattctgg tatttgcgga taaatttgtt
      421 ggatttcctt taacgttttt aatcggttat ctcctttttg cccatctgct agaggacgaa
      481 accacatgtc cattttcaat cccataagta cagctggaac aacacaaatc gtagaaacaa
      541 ctaaacgtgt gcctaaactt gttgttgggg tcaaaggatt ccaaaaacca gttgaatcaa
      601 aatggaataa ctgcggcatt tcttgtaact gaaaaatatt cactccttgt aaacttcctg
      661 gaataatctc aaatcgatga aatgcttctg gtattcttgt aacaacatag tttaaaggaa
      721 gataagctaa gccactataa aaaatctttt gagaaatacg tttgtcatgt ttttctggta
      781 gctcttctcc tgaaggagta tagcgataga aatgagaaag atacctattc ttttctaaat
      841 tgaatcgttt gtcttttcgt ctcgttgttt ctctaacacg cattatttca cctcatttga
      901 ttctgaactt tcggaagtgt ttgattgtac agttgaatcg cttgatcctt ctttagcctt
      961 actaggatca ctctctttgc tattttccat acttgattct gttgttgact gattggtacg
     1021 attcaataat tcttctaatt gttgttcata ctcttttctt gcttgttctt gggcttcttc
     1081 cttgattgac gtcgttgtgg ccacagaatc agcctgtgaa gattgattga tatgattaaa
     1141 agcaaatact gttcctccac ctagaagcaa gcttaaactt gcaatacatc ccaccatcca
     1201 tccgttaaag gaacgctgtg gtgaaggagt tagatttgtt tggttgttca ttggggtttg
     1261 aacagcagct actgaaggcg ttggttgagg ttgtgctaga taatttaact gagcttgtcg
     1321 caatgtatcc atttgttctt gacgttcctt acgagcagct gcttcttttt ccttttcttg
     1381 ctctaaccgt tgtttttcta tcgccatttt ttgttccgca agatcctgtt cccgttttct
     1441 tatttctaat tgttcacgct ctttttcttt ttgccgttgt ttttcttctt gttctttcaa
     1501 ttcttgttgt tgaatttcat actc
//
LOCUS       DACEKQ010000202         1506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEKQ010000202 DACEKQ010000000
VERSION     DACEKQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.215"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..108
                     /locus_tag="IW671_002845"
     CDS             <1..108
                     /locus_tag="IW671_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0899791.1"
                     /translation="VIVPKTGSTHLVTISAVSLLLLVLATFSYAVLRYI"
     gene            135..599
                     /locus_tag="IW671_002846"
     CDS             135..599
                     /locus_tag="IW671_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899792.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            596..838
                     /locus_tag="IW671_002847"
     CDS             596..838
                     /locus_tag="IW671_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899793.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            856..1158
                     /locus_tag="IW671_002848"
     CDS             856..1158
                     /locus_tag="IW671_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899794.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1228..>1506
                     /locus_tag="IW671_002849"
     CDS             1228..>1506
                     /locus_tag="IW671_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899795.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGF"
BASE COUNT          544 a          212 c          314 g          436 t
ORIGIN      
        1 gtcattgttc ctaaaacagg ttctacacat ttagtaacga tttcagcggt cagcttacta
       61 ttacttgtgt tagcaacttt tagttatgcg gttctgagat acatttaaaa taaaaaaacg
      121 aaagaatgaa taaaatgaaa aagttaataa tagtagttac ttccttgatt gccatgattg
      181 ttattttagt cggatgtagt aatgaaaaaa ataaacaaac taatcaagat aatggcgtat
      241 tgaaatcagg aactatgtgg aaagaagaag taggtggtct tgtttataat ttgaaaatta
      301 tagacgagac tacctgggaa tactctgaga gtgtatggca tccagatcct gtacaaataa
      361 cagtcaagag gcaaaaagac tataaaggtt tagaaagata caaaatagta gattcagctg
      421 gtgttaggga atttataaac aaatctgatt ctttatttat tgttgttccc tatgagaaga
      481 atggcgtaaa aaaaataatt tttcttggat ctagcaagga tgaaaagcga acgaaagaga
      541 aattgattca cgatggatca caatccaata aatataagtt acaaaagact agcgagtgat
      601 tgctagtctt tttatttgtc ctgaaggagg aatcacagtg gcaaaagcta aaacgccatt
      661 tgaaaaagcc gcgtctaaaa tcttagaacg ccgcggtgta tctgaaacac agtggaaaca
      721 agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg
      781 atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa
      841 ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta
      901 gtagctgcgg ttgatatttt tagagcatta caaactgccg caactagttt aaaaaacaat
      961 ctgatgattt ttgctgctgc tgcggccgtt gcttccattg ttattggggg tcttttcttc
     1021 ctatttggac gaagaggagc tgaaaatggg aagatgcacg taggctatgt attgatgggg
     1081 gtaggagtta ttttagcagc agcttctatt gtggtttcgt tatttggatt gtttggacaa
     1141 acacctcaag gattataaga aagtaaaaaa gtaggagtaa gaagtagtcg atacttttta
     1201 ctcctttttt atgaaaggag aatactcatg tactttacga ttgcaggcaa taccaaaaca
     1261 gataaagagt taataaaaaa agcaacgaag gaagtaggaa agcgtacttc ttttttaccg
     1321 aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt agggaaagta
     1381 ctggaacacg aaagtcgatc ttctgttaag ttgactctca cgttacatga cacagaagga
     1441 aaaatagaga aagctggtac agaatacgtt tttggcccac tggattatca aatttcagaa
     1501 ggtttc
//
LOCUS       DACEKQ010000203         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEKQ010000203 DACEKQ010000000
VERSION     DACEKQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.218"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..1041
                     /locus_tag="IW671_002850"
     CDS             109..1041
                     /locus_tag="IW671_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0899796.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IW671_002851"
     CDS             1113..>1457
                     /locus_tag="IW671_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899797.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACEKQ010000204         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEKQ010000204 DACEKQ010000000
VERSION     DACEKQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.219"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..1392
                     /locus_tag="IW671_002852"
     CDS             100..1392
                     /locus_tag="IW671_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0899798.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACEKQ010000205         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEKQ010000205 DACEKQ010000000
VERSION     DACEKQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.220"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW671_002853"
     CDS             complement(150..1337)
                     /locus_tag="IW671_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0899799.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEKQ010000206         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEKQ010000206 DACEKQ010000000
VERSION     DACEKQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.221"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1307
                     /locus_tag="IW671_002854"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="IW671_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0899800.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          595 a          103 c          233 g          454 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagt
//
LOCUS       DACEKQ010000207         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEKQ010000207 DACEKQ010000000
VERSION     DACEKQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.222"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1364
                     /locus_tag="IW671_002855"
     CDS             82..>1364
                     /locus_tag="IW671_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0899801.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACEKQ010000208         1359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEKQ010000208 DACEKQ010000000
VERSION     DACEKQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.223"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1247)
                     /locus_tag="IW671_002856"
     CDS             complement(48..1247)
                     /locus_tag="IW671_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0899802.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT          496 a          243 c          254 g          366 t
ORIGIN      
        1 tctaagattt ctctttacca acatcatttg acaaagagcc tgttttttta gaaaagtaaa
       61 gtaatcagcg ataagaatgc ggagcctgcg aaaagcaatc cgcaaatgat catatgtctt
      121 tttttgttca tcatcaagat ggcgaggtat tctcttaaaa acgcattagg caagtagtag
      181 aaagctgttt ttgcaccaat accatttgct ttcaatttgg cttgccgcgc ataaagaccg
      241 gctctaaaaa tgtggtaatt gttggaacta aagatcgcgc ggtatggccc tttcatttgc
      301 tgatccatga tttcctttga atagagcatg ttttccaaag tggttgtcga attggtttcg
      361 acaagaatgt ctctttcagg gattccttgt tccatggcat attgtttcat cgcaatggcc
      421 tctggtactt tttcgtctgc tccttgcccg ccagacatca ataagatggg cggatttaat
      481 gtagcacggc tttgagcccg ataaaaagca attgccttat tgattcgctt ggctagaaga
      541 ggagaaactg tttctccatt gatcaatcca gcgcctaaca caacgatgaa gtcttgattg
      601 tatcgaggcc ggttgaactg atacaagaat gagacagtca aaaagttgta gaatacgata
      661 aatagataga tcaagatcag gggaacgcag aaaaacaaag catttatcca ttgcggcaaa
      721 tattttaaca agaaaaagtc aaaaacaaga aacagtgtaa gaaaaatggc tagtatcaac
      781 gttaaaagat tagctaatga atgcgattct ctacgtaaaa ctgtgacccc gttccaaaaa
      841 agaaaaacaa tcaatccata aataccaaat aaaaggacaa gtagcaatgg aatcgtgatc
      901 aaggcgatca gtccgccaag aaaaatattt tgcgtttcat acagcaagac aaacaagtag
      961 atgccgaaac tgatgagtaa aacattgaat aaaagaccat tcagtagcct tcgtttttct
     1021 gtgaaataag aaaaagcaaa gatactaaag aacaacaatg gaatcaaaaa ataaaaggtc
     1081 gaataaaaaa tttccaacac gacaaaataa gttaagatta ccgtaaatgt ccacgtactg
     1141 ccgtaaaaga acaaggaagg atccttcttc tgttttcgcc aataaacgat agagaatacc
     1201 ccaataagat agagaactaa aaagatcaga tctctggtta gattcataaa ttcaactcct
     1261 ttttcttata cagtataacg aaaatagatg aaaaaagctg ttatttaaac tagaaaaagc
     1321 aaaaaaacat ctttccgatg gaaagatgtt tttcttatt
//
LOCUS       DACEKQ010000209         1358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEKQ010000209 DACEKQ010000000
VERSION     DACEKQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.224"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1358
                     /locus_tag="IW671_002857"
     rRNA            <1..>1358
                     /locus_tag="IW671_002857"
                     /product="23S ribosomal RNA"
BASE COUNT          363 a          295 c          417 g          283 t
ORIGIN      
        1 ggacaagctg tgatggggag ggaaataata gtaccgaagt tcctgatgtc acactgccga
       61 gaaaagcttc tagtgagaaa acagcggccc gtaccgcaaa ccgacacagg tagtcgagga
      121 gagaatccta aggtgagcga gagaactctc gttaaggaac tcggcaaaat gaccccgtaa
      181 cttcgggaga aggggtgctg atcatacgat cagccgcagt gaataggccc aagcgactgt
      241 ttatcaaaaa cacaggtctc tgcaaaatcg taagatgaag tataggggct gacgcctgcc
      301 cggtgctgga aggttaagag gagtgcttag cgcaagcgaa ggtacgaatt gaagccccag
      361 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc
      421 cgacccgcac gaaaggcgta acgatttggg cactgtctca acgagagact cggtgaaatt
      481 ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta
      541 ctgtagtttg atattgagtg tctgtaccgc atgtacagga taggtaggag ccgtagaaat
      601 cggaacgcta gtttcgatgg aggcgctggt gggatactac ccctgcgtta tggccactct
      661 aacccgcacc actaatcgtg gtgggagaca gtgtcagatg ggcagtttga ctggggcggt
      721 cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc
      781 gaagagtgta aaggcagaag ggagcttgac tgcgagacca acaagtcgag cagggacgaa
      841 agtcgggctt agtgatccgg tggttccgca tggaagggcc atcgctcaac ggataaaagc
      901 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      961 cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg
     1021 cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
     1081 gtcgcgggcg ttggaaattt gagaggagct gtccttagta cgagaggacc gggatggact
     1141 taccgctggt gtaccagttg ttctgccaag ggcattgctg ggtagctatg tagggaaggg
     1201 ataaacgctg aaagcatcta agtgtgaagc ccacctcaag atgagatttc ccatttcttt
     1261 aagaaagtaa gatccctgag agatgatcag gtagataggt caggagtgga agtacagtga
     1321 tgtatggagc ggactgatac taatcgatcg aggactta
//
LOCUS       DACEKQ010000210         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEKQ010000210 DACEKQ010000000
VERSION     DACEKQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.225"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            11..1350
                     /locus_tag="IW671_002858"
     CDS             join(11..452,452..1350)
                     /locus_tag="IW671_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0899803.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACEKQ010000211         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEKQ010000211 DACEKQ010000000
VERSION     DACEKQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.226"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..1310
                     /locus_tag="IW671_002859"
     CDS             15..1310
                     /locus_tag="IW671_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0899804.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          418 a          268 c          260 g          402 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
      841 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
      901 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
      961 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1021 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1081 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     1141 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     1201 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     1261 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     1321 aagaggtttt cttctttctg ttctgtcc
//
LOCUS       DACEKQ010000212         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEKQ010000212 DACEKQ010000000
VERSION     DACEKQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.227"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(146..1129)
                     /locus_tag="IW671_002860"
     CDS             complement(146..1129)
                     /locus_tag="IW671_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0899805.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          286 c          254 g          419 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catctgcggt aataag
//
LOCUS       DACEKQ010000213         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEKQ010000213 DACEKQ010000000
VERSION     DACEKQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.229"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..1303
                     /locus_tag="IW671_002861"
     CDS             35..1303
                     /locus_tag="IW671_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0899806.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          324 a          236 c          324 g          444 t
ORIGIN      
        1 tagtaggcgt ttttttattg aggaggaatt ttttatggaa aaacaattac cgagtccagt
       61 acgcatttta gtgtatatgc tgaagtggct ggtgatcaca gcgattctcg gtatattcat
      121 ggggagcttg tctgcttttt tcttaaatag cctgacagtt gtgacagata tccgactatc
      181 acatccttgg ctcctttgtc tactaccagt tagtggtgcg ctttttgctt atctctatgc
      241 gtatcacggc ggactttcta gtcgtggaaa caacttagtg atcgatcaag gaaatggtgg
      301 agaagaaaaa atcccgttgc ggcttattcc acttacactg tttggaacca taactaccca
      361 tttatttggg ggatctgttg gccgtgaagg aacagcagtc caaatggggg gagcgttagc
      421 agataatacg gcacgtttgt tccgattgga taaatcagaa cgggaaatct tgattatcag
      481 tggaatcagt gcgggtttta gttctgtatt tggcacacct ttagctggaa cattatttgg
      541 attagaagtg ttggcaatcg ggaaagtccg aacagaagcg atcttcccta gcttctttgc
      601 tgcactgttt gcaaatttca tgacagaaag ctatggtgtt tctcatctac attaccaaat
      661 gggtgtgatt cctgaatgga gtatgttgct gttcttcaaa gtttttgttg ctgggattgc
      721 ttttggtttg attggttggg tattcagccg ttcgatcgtc tttttaaaag cgcgatatgc
      781 acaatggatt cctcatcctg tttgtcgaaa tacgataggt ggagctgtag ttgtggcagc
      841 agcattgatt ttgcaaacac aacgttatct cggattgagt cttccgttac ttcaagaatc
      901 atttaatgga caggcacatg tctatgattt tattgggaag ttattcttca cagttttgtc
      961 tttaggcgcg ggatatcaag ggggagaagt gacaccattg tttgaaattg gtgcaacgct
     1021 tggtgcagca ctagcgcctt tgctccatgt ttctgttcct tttcttgcag gattgggctt
     1081 tatcggagta ttttctggtg cgacaaatac accgattgcc tgctttgtga tggggatcga
     1141 attgttcggt tcacaagcgg cagtatactt ctttatgatc tgcttgatca gctttatgtg
     1201 ttcaggcaac agcggtattt attctgctca gcaagtagcg gtcaaaaaag ggatactctt
     1261 tttgccaata gttaagagaa tgaaagaaaa aaacaaggtg tgacatatgt tttgtcacac
     1321 cttgtttt
//
LOCUS       DACEKQ010000214         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEKQ010000214 DACEKQ010000000
VERSION     DACEKQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.230"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..>1310
                     /locus_tag="IW671_002862"
     CDS             129..>1310
                     /locus_tag="IW671_002862"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0899807.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          410 a          262 c          236 g          402 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gctccttatt tctcactagt taaagagtgt tttccaaatg
      841 cacaagtgat cgtcgatcgg tttcacattg ttcaacatct gaaccgcttc ttcgatgcgg
      901 ttcgaaaacg tgtgatgaaa acacttgatc aaaaagaccc aatacaagct aaacagtatc
      961 gccaattgaa atccttattc aaattacttt taaaatcaga ggatcgttta gattacaata
     1021 cactaacgaa aagacgcaat ttcaattgga gactattgac tgaaacagaa gtagttgacc
     1081 gccttctaaa gatttctcct gaacttaaaa cagcttatcg ttactaccaa gacctgctta
     1141 ctgcgtatca ggaaaaagat ccagatactt ttttccagct gcttcgagca atgcctggag
     1201 aagttccatt ggaactgcac catatcaaaa aagccttttt caaatacgag aaagggattc
     1261 ggctggcatt actgaagaag tattcgaatg cacgactgga aaatctccat
//
LOCUS       DACEKQ010000215         1263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEKQ010000215 DACEKQ010000000
VERSION     DACEKQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.232"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1004
                     /locus_tag="IW671_002863"
     CDS             132..1004
                     /locus_tag="IW671_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0899808.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          384 a          229 c          289 g          361 t
ORIGIN      
        1 aaatgataaa aattcaaaaa gaaaaaatag tagttattta cttgtcatgt tactaaataa
       61 aaaaataatt taaatatgat atatagtcat ttatttagtg atttcattct aaaataaagg
      121 gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg cacaaaattt gtgtgtgcca
      181 ttggcgatga agaaatgacg atcaaggaaa gagtgagctt tccgacaacg acacctgagg
      241 aaacaatgcc tttagtcatt gacttcttca aacaatacca agcagattta gcagggattg
      301 ggattggctc atttgggcct atcgatatcc atcgcgattc cgccacttat gggtacatta
      361 cctcgacccc gaaattggca tggcaaaatt ttgatttcat cggtaccatg aaaaaagaat
      421 ttccgatccc gatttcttgg acaacagatg tcaatgcggc tgcctatggt gagtatgtct
      481 tcggcagtgg caaaggattg agcagtgtcg tttactatac gatcggtacg ggtgttggcg
      541 gcggtgcctt acaagaaggg cgctttatcg aagggtttag tcatccagaa atggggcata
      601 tgctcgtcgt ccctcatcca aaagatgact ttgctggcag ctgtccattt catggcaact
      661 gtttagaagg gatggccgct ggacccgcca tcgaaaaccg cttagggaaa aaaggacaag
      721 aggttgcaga agatgatcct tattgggaaa tcgaagcctc ctatattgct caatgtgcct
      781 acaatacaac cttgatgttt tctccagatg tgatcatttt tggcggtggt gtgatgaaac
      841 aacgccatat gctgcaaaac gtccacgacg catttgcaaa attggtcaat gggtatgtcg
      901 aaacacctga gttaagcaaa tatatcgtga ccccagcatt agaagacaac gctggaacgc
      961 tcggttgttt ggcattggct cgagaagcgg tcttacattc ataaacgaaa aaaggctctt
     1021 cgacatttag tgttggtaga gaaagataca actgaaaatt tagtcataac cagttaggca
     1081 gagtgcttaa ctggtttttg ttttacttgg aagatcaagc cttgaatgag ataaattctt
     1141 gctcgaaagt tatggaaatt tcgatagccg taggccactt gtattgtgca atagtttttt
     1201 acaaaaaaaa gggggtgcgg atattttttt ggaccttttt aacaaatata tctttgcttc
     1261 ttc
//
LOCUS       DACEKQ010000216         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEKQ010000216 DACEKQ010000000
VERSION     DACEKQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.234"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1246
                     /locus_tag="IW671_002864"
                     /pseudo
     CDS             200..1246
                     /locus_tag="IW671_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          514 a          141 c          237 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACEKQ010000217         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEKQ010000217 DACEKQ010000000
VERSION     DACEKQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.236"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW671_002865"
     CDS             414..947
                     /locus_tag="IW671_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0899809.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEKQ010000218         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEKQ010000218 DACEKQ010000000
VERSION     DACEKQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.237"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1134
                     /locus_tag="IW671_002866"
     CDS             <1..>1134
                     /locus_tag="IW671_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899810.1"
                     /translation="PYYDGGRGSVKDMVAGGLGAVGAQGIADRLNNMGDSNADTKTIA
                     DKVVPETPASVPFNGGGVGGTINNTSFKDLVRQDQLRNNSQQYSDHLGRMVDEKGNFV
                     DENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPLEDNEDGNIGMDSQGRL
                     IDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRLVDEDGNLVDEFGKKLG
                     KHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPIAVAIPDDESDEVILDQ
                     AHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNVPVEGQSNELHNNFDNV
                     ADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVNQA"
BASE COUNT          419 a          167 c          270 g          278 t
ORIGIN      
        1 tccttattat gatggtggca gaggatcagt aaaagatatg gtcgcaggag gacttggagc
       61 agtcggagcg caaggaattg ctgatcgttt gaacaatatg ggagacagca atgctgatac
      121 taagacaatc gctgataaag ttgttcctga aacgcctgct tcagtaccat ttaatggtgg
      181 cggagttggt ggaacaatca ataatacctc tttcaaagat ttagttcgtc aagatcaatt
      241 acgtaataac tcgcaacaat atagcgatca tctaggacgg atggttgatg aaaaaggaaa
      301 ctttgtcgat gaaaatggtg aagtattaaa agacagtaaa tttggtaaag atgccaaagg
      361 aaactttatt gatgacaaag ggcgtttggt cgatgaagat ggaaatttga tagatacaaa
      421 cggtaaacca ttggaagaca atgaagatgg aaacatcggt atggatagcc aaggacgttt
      481 aattgatgac aaagggcgct tggtcgatga cgaaggaaac cttgttgatg aaaacggacg
      541 taaattactt aacggtgatt ttgggcagga tgtaaatggt cgtctagttg atgataaagg
      601 acgattagtt gatgaagacg gaaatctggt cgatgagttt ggtaagaaac ttggaaaaca
      661 cagtaacggt tataacgtaa atggcgatac agtcgatgaa gatggaaaca tcattgattc
      721 aagagatgct cttgtagcag atcgaaataa agaactcgaa caacaacagc atacaccaat
      781 tgcggttgca atccctgatg atgaaagcga tgaagttatt ttagatcaag cacatcaagg
      841 gttaagagaa gaagcaaagg ataaagttgt ttcagatcaa gcacaagaag agctaaaaaa
      901 taaatcgaat ggtgaagatg tttcaaacca atatcatcaa acggctggaa atgtacctgt
      961 tgaaggtcag tctaatgagc ttcacaacaa ctttgataat gttgctgatc ggaatgatac
     1021 agtcttaaaa gaaaaagaat cattaagaca agaaacagat aaagaccaat caaaattgaa
     1081 gaaaacaact attcctactc ctattggttc aacagttccg aaggtcaatc aagc
//
LOCUS       DACEKQ010000219         1126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEKQ010000219 DACEKQ010000000
VERSION     DACEKQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.238"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            56..129
                     /locus_tag="IW671_002867"
     tRNA            56..129
                     /locus_tag="IW671_002867"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:90..92,aa:Ile,seq:cat)
     gene            509..670
                     /locus_tag="IW671_002868"
                     /pseudo
     CDS             509..670
                     /locus_tag="IW671_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            820..1020
                     /locus_tag="IW671_002869"
     CDS             820..1020
                     /locus_tag="IW671_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0899811.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT          357 a          177 c          239 g          353 t
ORIGIN      
        1 ttgttgccac accttctact ggttgattgt ttatggcttt atgtggcaac aacctgtact
       61 cttagctcag ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
      121 cagggtacat aatttaatct atttgataaa aaataaaatt caataatggt ataataaaat
      181 gtcggctcgt cgcttccaca gagattttcg tgggaggagg tgtatgattt gtttgaattc
      241 actttggagc acgtgctctt gccattgtta attggtttaa ctttggcatt atttagtcat
      301 tggctagata aacattagag ccgacaatgg cgcttactgt ttcgcagcgg taagcgtctt
      361 tttttgaaaa agaaaaaaac acaacgtatt cgcaggtacg ttgtgtttat ggtgtatatt
      421 tgtttaaatt cactttcaat tattattata cttgttaaat aaattgtgtc aatataaaag
      481 atatttttat acgtgtacta acctatatta tccttctggt aatgttattc catcgcagca
      541 ggatatgaat tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact
      601 ggaccacttg atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga
      661 agagaagtaa gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt
      721 ttgtttggga atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac
      781 aatttaaatt agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa
      841 tggttcaacg ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc
      901 gttcacttct cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg
      961 actttcgacg ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa
     1021 ttttatctgt aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta
     1081 acaaaaatac ctgccacaag agacaatttc tcttgtggca ggtatt
//
LOCUS       DACEKQ010000220         1118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEKQ010000220 DACEKQ010000000
VERSION     DACEKQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.239"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..>1118
                     /locus_tag="IW671_002870"
     CDS             175..>1118
                     /locus_tag="IW671_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899812.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTR"
BASE COUNT          443 a          176 c          223 g          276 t
ORIGIN      
        1 aattcaactg tttatcaaga agcaaaagaa cgagtggaag gaaaaattaa atccaatcaa
       61 acaaaaaaat attcccctca gttgaagaaa cctgtgaatg aatctgttca tgccagagta
      121 gatccatttg atccaacgaa gattctaaaa agataaaaat gaaggaggat aataatgact
      181 caatcgcctt cagttattct cacttgtcaa tttactttgc cgaataaaaa gtcattttct
      241 acttatattg actatattac aagggaacag gctcttgaag aaaaggaacg acgtacgccc
      301 gaagaagaac aggaacttca acgaatcaag aatgctttag aggagtttta tattcctgaa
      361 ggagaaactt tttcggagag taagacgggt aaagaattca gtgaaacgtc gaatgaagct
      421 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaat
      481 tatctttctt atatgacacg tcactatgcg ttagaacaaa agaaagaact gacgcaaagt
      541 gaacaaaaag aataccaacg cttgaaccaa gcaatgaaaa aatatcaaac agaaccccat
      601 aaaaaagctt caggaaatct tcctggagtt ttttctatag cttcagacga agtgaaagga
      661 agtgatctga aggagattaa gcaaatcttc caaagaggac aacaaaaagg ctcaatcatt
      721 tatcaagatg ttgtttctca tgataattcg tatcttgaaa aaataggatt gtacgatccc
      781 aaaacagata ctttagatga agaagggtta aaagcagctg gaagaaaaat gatggagaca
      841 ttattcgaga aagagcaact gaacgaaaca ggctactgga tggcaacgat tcaccgtaac
      901 actaaacata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccttac
      961 acggaggttg aaaatggtgt gagttatatc gtaccgaaag gtaagagaag acaagcaaca
     1021 ttagacgcaa tgaagaccaa ttatgtacat gagttagaac gtttttctta tgaaaaggat
     1081 cgaatgattg gtatggagaa acgaaattta ttgacgcg
//
LOCUS       DACEKQ010000221         1108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEKQ010000221 DACEKQ010000000
VERSION     DACEKQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.240"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..>1108
                     /locus_tag="IW671_002871"
     CDS             175..>1108
                     /locus_tag="IW671_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899813.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRN"
BASE COUNT          444 a          180 c          223 g          261 t
ORIGIN      
        1 aattcaacgg tttaccaaga agcaaaagaa cgtgtggaag ggaaaattaa atctaatcaa
       61 actaaaaaat attcccctca gttgaagaaa cctgtgaatg aatctgttca tgccagagta
      121 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
      181 aaatcgcctg cagtcattct cacttgtcaa tttaccttgc ccaataaaaa atctttttca
      241 acatacattg attatatgac aagagaaaaa gcgctggaag aaaaggaacg acgcacgccc
      301 gacgaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
      361 ggagaaactt tttcggagag taagacggat aaagaattca gcgaaacgtc gaatgaagct
      421 caatctttga tgggaaatgg aatcaacttt gacaagttag aggaacaaga ttttaccaaa
      481 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagt
      541 gaacaaaaag aataccaacg cttgaaccaa gcaataaaaa aatatcaaac agagccacac
      601 aaaaaagctt caggaaatct ccctggagtt ttttctatgg cttcagacga agtgaaagga
      661 agtgatctga aggagattaa gcaaatcttc caaagagggc aacaaaaagg ctcaatcatt
      721 tatcaagatg ttgtttctca tgataattcg tatcttgaaa aaataggatt gtacgatccc
      781 aaaacagata ctttagatga agaaggatta aaagcagctg gaagaaaaat gatggagaca
      841 ttattcgaga aagagcaact gaacgaaaca ggctactgga tggcaacgat tcaccgtaat
      901 accaaacata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccttac
      961 acggaggttg aaaatggtgt gagttatatc gtaccgaaag gtaagagaag acaagcaaca
     1021 ttagacgcaa tgaagaccaa ttatgtacat gagttagaac gtttttctta tgaaaaggat
     1081 cgaatgattg gtatggaaaa acgaaatt
//
LOCUS       DACEKQ010000222         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEKQ010000222 DACEKQ010000000
VERSION     DACEKQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.241"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..980
                     /locus_tag="IW671_002872"
     CDS             6..980
                     /locus_tag="IW671_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0899814.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACEKQ010000223         1088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEKQ010000223 DACEKQ010000000
VERSION     DACEKQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.242"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..275
                     /locus_tag="IW671_002873"
     CDS             <1..275
                     /locus_tag="IW671_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0899815.1"
                     /translation="NPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            618..874
                     /locus_tag="IW671_002874"
                     /pseudo
     CDS             618..874
                     /locus_tag="IW671_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          376 a          163 c          198 g          351 t
ORIGIN      
        1 gtaatccctt cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt caactttgcc
       61 aagaaaaagg gccatttata aaagacggtg taccttattt acattgtcat catatagaat
      121 atttgtcgca aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt cccaattgtc
      181 atgctagaat tcatgaactt gagttacctg acgataaaga aaaattgctg aagatcgttg
      241 aaaaaagaga aaatagcttt tctaaaatac cgtaatttat ttttatagga caaataagct
      301 aatgtgagct atgccttcta tataagacta agttctatta taagtaacga agtaattctc
      361 gttccttcct gtagtaatat tttttaaata acctataggc ttcttcaaaa gtacatcctc
      421 cagtaaattc taagtactgg aggattcttt ttctagtgtt gctttactca tgaaaaagta
      481 gttggctact tcatcaaaat ttctgaactt ttttaatgta taataaagag ctcgatcatt
      541 tactacaagg atgctagctc ctacattttc acagtttttt agcaagtaaa tttttctaaa
      601 atcacatcag gaaatgcatg agattcttta agaaaggcaa ttatagggct atgattacgg
      661 ttcgtgtaag tgacgccaaa aaagaatggc tttcttatat ggctgaattt tatgggatta
      721 ctttatctga acttctaaaa aactattcga tggaacaact agaagatgaa tacaatcgtc
      781 aaacggctga aattgcccat aaacattgga taagaagtgg gaaaacaaca gtattcatgg
      841 aagaatcctt aatgaatttg gtgggctgga atgacctgcc gtttaaaatt cacgcccgaa
      901 attcagaagc agctctgatt ggcaaatacg tgtattggta gagatagaag ataatcaatt
      961 cgttgttacc gctattcaag tagggcaccg aagaaatgtc tatgattaag agactgttta
     1021 tttttacaaa aaaataataa ctggaatcta gttaaaactc ttgaagtgag agatttgact
     1081 agattttt
//
LOCUS       DACEKQ010000224         1079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEKQ010000224 DACEKQ010000000
VERSION     DACEKQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.243"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..205
                     /locus_tag="IW671_002875"
                     /pseudo
     CDS             59..205
                     /locus_tag="IW671_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="IW671_002876"
     CDS             282..611
                     /locus_tag="IW671_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0899816.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="IW671_002877"
     CDS             601..888
                     /locus_tag="IW671_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0899817.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          396 a          135 c          197 g          351 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataag
//
LOCUS       DACEKQ010000225         1060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEKQ010000225 DACEKQ010000000
VERSION     DACEKQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.245"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            77..>1060
                     /locus_tag="IW671_002878"
     CDS             77..>1060
                     /locus_tag="IW671_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0899818.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIY"
BASE COUNT          387 a          201 c          179 g          293 t
ORIGIN      
        1 aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac caattcacag
       61 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
      121 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      181 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      241 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      301 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      361 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      421 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      481 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      541 taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc aaaccgtatc
      601 caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag aagtactgag
      661 ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac aggatattaa
      721 ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag gcatgaaaga
      781 tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc atgtatctcg
      841 taacatcgct cataaagtac gtgtcaaaga ccgaaaagaa atctgtgatg actttaaggc
      901 tgtttatcaa gctaactcaa aagaagaagc gaataccttc ttatccggca tgattgagaa
      961 atggaagaaa aactatccta aagtgacgca gtcactcata gaaaaccaag acttattaac
     1021 tttttatgat tttccaccta gcattcgtag aaccatttac
//
LOCUS       DACEKQ010000226         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEKQ010000226 DACEKQ010000000
VERSION     DACEKQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.246"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(36..992)
                     /locus_tag="IW671_002879"
     CDS             complement(36..992)
                     /locus_tag="IW671_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899819.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          345 a          176 c          159 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtata atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACEKQ010000227         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEKQ010000227 DACEKQ010000000
VERSION     DACEKQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.247"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(129..776)
                     /locus_tag="IW671_002880"
     CDS             complement(129..776)
                     /locus_tag="IW671_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0899820.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT          284 a          193 c          167 g          366 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
//
LOCUS       DACEKQ010000228         1004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEKQ010000228 DACEKQ010000000
VERSION     DACEKQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.248"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            52..307
                     /locus_tag="IW671_002881"
                     /pseudo
     CDS             52..307
                     /locus_tag="IW671_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1004
                     /locus_tag="IW671_002882"
                     /pseudo
     CDS             371..1004
                     /locus_tag="IW671_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt acct
//
LOCUS       DACEKQ010000229         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEKQ010000229 DACEKQ010000000
VERSION     DACEKQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.249"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..979
                     /locus_tag="IW671_002883"
                     /pseudo
     CDS             1..979
                     /locus_tag="IW671_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          339 a          197 c          194 g          272 t
ORIGIN      
        1 gaccgagtac ggggaacttc atctccagat tccgcgcgac cgcaacggcg agttcaagca
       61 acagactgtt cctgcttata gacggacgaa tgatatctta gaggagaccg tcattcacct
      121 cttccgaaaa ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca
      181 tcactacacg ccccaaacca tgtccaacat gactaaagtg ctgactgaag aagtaaatgc
      241 atttaaagtt agaactctaa atgataagta tgtcgctatt ttcatggatg ctacttatat
      301 tcctttaaaa cggcaaaccg tctccaaaga agcaatctat attgccattg gtatacgaga
      361 agacggcact aaagaagtac taagttatgc gattgctccg actgaatcga cctacgtttg
      421 gaatgagttg ttacaggaca tttactccag aggggttcat gatgtcttac tgtttattac
      481 cgatggctta aaaggcatga aagatacgat tcaccagatt tatcctaaag ctaaatacca
      541 acattgctgt gttcacgttt cccgtaatat tgctcataaa gtacgtgtca aagatcgaaa
      601 agaaatctgt gatgatttta aggctgttta tcaagctagc tcaaaggaag aggcaaatac
      661 ctttttaggg agtatgattg agaagtagca aaaaacttat cctaaagtga cgcagtcact
      721 gataaaaaat caagatttat tgacttttta tgagtttccg cctggtatcc gccgaagcat
      781 ttactcaact aatttaatcg aatctttcaa gaaacaaatc aagaaataca gccgcagaaa
      841 agagcagttt caaaatgaag aatcactaga gcgtttccta gtatccatct ttgatacata
      901 caatcaaaaa ttcttaaata gaagccataa aggcttccaa caagtgacgg atacattagc
      961 ttcaatgttt actgagtaac ccattatttt gcagaaggac ga
//
LOCUS       DACEKQ010000230          992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEKQ010000230 DACEKQ010000000
VERSION     DACEKQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.251"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..496
                     /locus_tag="IW671_002884"
     CDS             179..496
                     /locus_tag="IW671_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0899821.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(587..952)
                     /locus_tag="IW671_002885"
     CDS             complement(587..952)
                     /locus_tag="IW671_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899822.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          367 a          128 c          168 g          329 t
ORIGIN      
        1 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
       61 gtaacgataa tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact
      121 atggtattat ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt
      181 gcttttttat agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat
      241 ttttgatagg agtagaaatt caagtatagg ttttagaact aaacgaagtt tgtcttctaa
      301 aaaaaattgg gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact
      361 ttcttcaaca ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat
      421 tattggaatc attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa
      481 aagaagtaaa aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt
      541 ataaaaagaa gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag
      601 cttttttata agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa
      661 tacctgcaag aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt
      721 cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata
      781 aagatataat acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca
      841 atgtgatgaa cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat
      901 aaggaacaaa agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc
      961 accacctaac aagtgttatt attttgttag tt
//
LOCUS       DACEKQ010000231          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEKQ010000231 DACEKQ010000000
VERSION     DACEKQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.252"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..94)
                     /locus_tag="IW671_002886"
     tRNA            complement(21..94)
                     /locus_tag="IW671_002886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IW671_002887"
     tRNA            complement(108..181)
                     /locus_tag="IW671_002887"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IW671_002888"
     tRNA            complement(209..281)
                     /locus_tag="IW671_002888"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IW671_002889"
     tRNA            complement(301..374)
                     /locus_tag="IW671_002889"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IW671_002890"
     tRNA            complement(384..457)
                     /locus_tag="IW671_002890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IW671_002891"
     tRNA            complement(468..553)
                     /locus_tag="IW671_002891"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IW671_002892"
     tRNA            complement(570..641)
                     /locus_tag="IW671_002892"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IW671_002893"
     tRNA            complement(653..725)
                     /locus_tag="IW671_002893"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IW671_002894"
     tRNA            complement(743..824)
                     /locus_tag="IW671_002894"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IW671_002895"
     tRNA            complement(832..904)
                     /locus_tag="IW671_002895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IW671_002896"
     tRNA            complement(908..980)
                     /locus_tag="IW671_002896"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          282 c          235 g          214 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgaccttcc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACEKQ010000232          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEKQ010000232 DACEKQ010000000
VERSION     DACEKQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.255"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..969
                     /locus_tag="IW671_002897"
     CDS             94..969
                     /locus_tag="IW671_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0899823.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSDDKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPQR"
BASE COUNT          343 a          167 c          199 g          275 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa ctacttcaga tgataaacca gtcattgatc caactagagc aggtgcagca
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg agaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaatt ataaatacga gtatattttc
      721 attatggatt acaatactgg aaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa atcaagctta taatacttct ggaaacgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctaataa gaaagtctat gtaatgatgc gtgcaacgaa tgatccagaa
      901 gggaacacta aaggcggagc acaagatttt catgataaac gctggtattt aaatattccg
      961 caacgataaa aaaagcccct cgat
//
LOCUS       DACEKQ010000233          969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEKQ010000233 DACEKQ010000000
VERSION     DACEKQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.256"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..843
                     /locus_tag="IW671_002898"
     CDS             49..843
                     /locus_tag="IW671_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899824.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLGLAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMDKYRV"
BASE COUNT          388 a          114 c          232 g          235 t
ORIGIN      
        1 atgaaaccag ttattaaaat actagataac aaagagtgag tggaatatat ggtagcaaaa
       61 aagaatactc taaaagaagc ttctgaaaaa tatcaagata tgaaaagacg aggattaata
      121 ccaaaggaca aaaaaggtat atggagagaa tttgttaagg aagtcagtag tgatcgaagt
      181 ggcggtgact ttgttggacg atatacagca aaaggttata gcaggcgctt aaggatcact
      241 gttgaatatg gaacaaatga atcagctaca gagctgaagc aaaggtactt agatacacga
      301 agagaagctt caaaaaaata tgatgatttc aagagcagag gtctaattcc taagaagaag
      361 aaggggattt ggcgtgagtt tttcaaagaa gaagatagca atcggaataa gggagacttc
      421 attggacgat acacagcaaa aggctacact aagaatttag ggatcaaggt tgaatacgga
      481 actggtgaaa gttcatggga attgaggaac cgctatttaa atacattatt ggagaaatac
      541 aaagcagaat tattagagct gttcgatgtt aatgatttca tagagatggc tgaaaaatat
      601 ggtcatgaag aaggctttat gatcaatgac attatggtta ttgataattt tacggactta
      661 ttaggacttg cgaaaaaagc aagagattta agagaaacac agatgtatct attcagaaat
      721 aaaaacggaa gaaaccggag aactgaatcg agcgagatcg aagattacta tgatgccttt
      781 acacacgatc taggagcctt tgtagacgag atcagtggtg ttatggacaa atatagggtc
      841 tagaataaaa aagccggaga tgagcaaata aatgggaaat aatagcatat atacacctga
      901 aatgcagaat aaaattaaaa gacttgtcgg attagggtgg atgacaagag gtaagttaga
      961 ttcttttag
//
LOCUS       DACEKQ010000234          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.257, whole genome shotgun sequence.
ACCESSION   DACEKQ010000234 DACEKQ010000000
VERSION     DACEKQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.257"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW671_002899"
     rRNA            2..>943
                     /locus_tag="IW671_002899"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEKQ010000235          912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.260, whole genome shotgun sequence.
ACCESSION   DACEKQ010000235 DACEKQ010000000
VERSION     DACEKQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.260"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            31..>912
                     /locus_tag="IW671_002900"
     CDS             31..>912
                     /locus_tag="IW671_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0899825.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQD"
BASE COUNT          292 a          185 c          174 g          261 t
ORIGIN      
        1 caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcatat caaactacta
       61 cttggaatca aagataaaaa cattttcatc acaaatgtgg aatcgaagag cattaagaat
      121 atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg tcctttgtgt
      181 aaacagatga atcatgaagg catgatcgtt aagaacggaa agaaaaaatc gctcattcaa
      241 cttaataaat gtgcgaacca actgacttat cttgctttag cgaaacaacg ctaccactgt
      301 cgtggctgcc acacctattt tacggcgaat acctacattg tcgatagaaa ctgctttatc
      361 gctaaacaag tgcgctataa aattttagaa gagctaactg aaaaacaagc aatgacgaca
      421 attgctaagc attgcggcgt ttcttggtcg accgtttctc ggacactcgc ttctctgatc
      481 cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt tattaatgga tgaatttcgt
      541 tcactgaaaa atcaagtcgg tccctactcc ttttcttgta tggatggcga tactggaaaa
      601 ttactggata ttcttccttc acggaagaaa aaggctctcg tttcttattt tatgcaattt
      661 gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt ttcttatgcc
      721 tctctgataa aggaatgttt tccaaaggca aaactcgttg tggatcgttt tcacattgtc
      781 aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag ctttgatcga
      841 aacgatccaa cacaagccaa atattatcga caattaaagg cgttgtctcg gcttctgatc
      901 aaacgacagg ac
//
LOCUS       DACEKQ010000236          888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.261, whole genome shotgun sequence.
ACCESSION   DACEKQ010000236 DACEKQ010000000
VERSION     DACEKQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.261"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(308..643)
                     /locus_tag="IW671_002901"
     CDS             complement(308..643)
                     /locus_tag="IW671_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899826.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGNLVPYYTTWEMNEEGINNFPCKSI
                     KESISLTEC"
     gene            complement(664..>888)
                     /locus_tag="IW671_002902"
     CDS             complement(664..>888)
                     /locus_tag="IW671_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899827.1"
                     /translation="NERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLN
                     RTINAHEEKYHASKAYEEQQRRIQMEQERQ"
BASE COUNT          234 a          187 c          127 g          340 t
ORIGIN      
        1 tgcatcaacg aacgtcttcg tagtcagatt taacgttaaa atattgccac tttgataagc
       61 ttttgccgct ttgtctgtga cgacaaaagc tctgcccgtt tgaatttctg tcacaaaaac
      121 gagattatct ctgctttttt caacaacaac tccaatcgtt gaatgaacca tttcttcttc
      181 ctcccttctc tttcttttga tataatgtag tcgcaaatcg caccactcaa tgaattggcg
      241 gaaaacaatt cattgaaaaa aagaatcctg gacaatcacg ttcaggactc tttttttcac
      301 ttaataacta acactctgtg agtgaaatag actcttttat gcttttacat ggaaagttat
      361 tgattccctc ttcattcatt tcccaagtag tataataagg aactaaattc ccttcatatt
      421 cttgaatgat aaaatatttt tcacccttca catgattaca acaatacaca ccagtagctg
      481 ggtactcaac gactaaaaat tgatttggat caaactgttc gtttctcaac atttctctta
      541 tctggaacaa atgctctttt tctaaacaat caggaagttg ttcgattcgt tcttctctta
      601 atgatccttc tataactttc cattgatcta aatattgttt cattgctatt cctcctactg
      661 tctttactgt ctttcttgtt ccatttgaat tcttcgttgt tgctcttcgt atgctttact
      721 tgcgtggtat ttttcttcat gggcattaat cgttcgattt aattgacgca aactccgttg
      781 cgtgacaact ctttgttgga attgtttcct tcgcttcatg aattcttcgt aactatactt
      841 gtattcattt gttggcaact gtttaagtgt tcgaaaggat cgttcatt
//
LOCUS       DACEKQ010000237          797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.263, whole genome shotgun sequence.
ACCESSION   DACEKQ010000237 DACEKQ010000000
VERSION     DACEKQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.263"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            122..361
                     /locus_tag="IW671_002903"
     CDS             122..361
                     /locus_tag="IW671_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899828.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            473..667
                     /locus_tag="IW671_002904"
     CDS             473..667
                     /locus_tag="IW671_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899829.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
BASE COUNT          318 a           92 c          170 g          217 t
ORIGIN      
        1 aagaataaaa actggaggaa taaattttac agataaaggc ttagaagaag tcacgacgaa
       61 taataaaaag gtagtattac cttaggcatt gagtaaggaa ttcatttagg aggaaaatta
      121 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      181 acagctgtta gctcgtggtt attcacctaa agatgtattc aaatccctgt ctaaaggaac
      241 agatatggaa aaagaaagat tacgaaaaga attcgattat tggagaaccc ataatggaat
      301 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      361 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      421 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaat aaatggaaga
      481 aaagttgcta aaaacgatga aacaaaaaca ccttaaaaga ttatctgtca tgcaatatat
      541 caatgatatg cagataacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
      601 tgaacagttg agacgaactt atgtgaaaac aagttctaat tgtcaactat tgttagaagt
      661 ctcatgacat tcattaatag aattcatttt taaaactttt tctatggaaa tgagaaaaag
      721 attttttgtt ttgtctgaaa gggggtagct ggatgagaaa atggttattt ggttttttag
      781 gagtcgcttt aatcgtc
//
LOCUS       DACEKQ010000238          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.264, whole genome shotgun sequence.
ACCESSION   DACEKQ010000238 DACEKQ010000000
VERSION     DACEKQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.264"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW671_002905"
     CDS             <1..782
                     /locus_tag="IW671_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0899830.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          243 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc tt
//
LOCUS       DACEKQ010000239          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.265, whole genome shotgun sequence.
ACCESSION   DACEKQ010000239 DACEKQ010000000
VERSION     DACEKQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.265"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..346
                     /locus_tag="IW671_002906"
     CDS             83..346
                     /locus_tag="IW671_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HAQ0899831.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            346..681
                     /locus_tag="IW671_002907"
     CDS             346..681
                     /locus_tag="IW671_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0899832.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
BASE COUNT          310 a           84 c          158 g          237 t
ORIGIN      
        1 aaattgtttg atggtggaaa gtaacttctt gtattttggt gtatacaagt atatactaat
       61 gtgtgagaga aggagggatt ccatgttagt aaaagaaatc gaaatgaaaa aatggggaaa
      121 ttcacaaggt attcgttttg gaaaagaaga attgtcagtt ctaggagaaa ctaatactga
      181 aaatttaaaa ttcgaaatgg tggtaacaga aggaaaaatt gttttaactc ctaaaaataa
      241 aaagccagaa actttagatg aattgttcgc caattatgaa ggagaaccct taggaaaaga
      301 agacaaatat gaatggggaa atatgcaagg tagggagatc atttaatgaa aaagaactat
      361 catgatttat cgcaaggaga tcttgtttat attgatttaa atcctaccaa aggacatgaa
      421 caacggggaa aacgaccatg tattatttta tctaaaccta ataaatattt gaactatatg
      481 attggtgtcg caccgataac aacaaaagcg aagccttttc ctgtacatgt tgatttgaat
      541 ggtacgcaaa aaataaaagg acaaattctt ttggaacacc atcgaatgat tgacattgag
      601 agtagaggtt ttcagtttgt ggaagctgtg ccaaaggaaa ttttagaaga atgtattgaa
      661 aaaattaaat tattttatta aaagcagcga agaataatta ttatttcttc gcttttttgt
      721 tgtttaaaat taaaaagtta ggagtactga ctatatggaa aatgatacaa atatagttat
      781 ggaacattt
//
LOCUS       DACEKQ010000240          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.266, whole genome shotgun sequence.
ACCESSION   DACEKQ010000240 DACEKQ010000000
VERSION     DACEKQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.266"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(358..558)
                     /locus_tag="IW671_002908"
     CDS             complement(358..558)
                     /locus_tag="IW671_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0899833.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          281 a          120 c          117 g          247 t
ORIGIN      
        1 tatagctaaa attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa
       61 atctgcatat tcatcactaa tattttttct aataaattct tccatcataa agatcacctc
      121 gaaaagagta tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa
      181 taggtgaaca agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac
      241 aaacaaaaag aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg
      301 tgtaatagtt agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta
      361 ttttgtataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
      421 aagtatattt actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat
      481 aaacagtcga agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac
      541 ggtgtagttg attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac
      601 cagtcctatc caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta
      661 cgtaaagcca atgggaatga actggagatg actttttatt atttttagta atagctgatg
      721 ggttaatact tacaactttc cttttcaaag taccatataa aaaaa
//
LOCUS       DACEKQ010000241          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.267, whole genome shotgun sequence.
ACCESSION   DACEKQ010000241 DACEKQ010000000
VERSION     DACEKQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.267"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(237..761)
                     /locus_tag="IW671_002909"
     CDS             complement(237..761)
                     /locus_tag="IW671_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899834.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          261 a          102 c           85 g          316 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa aatctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aacc
//
LOCUS       DACEKQ010000242          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.268, whole genome shotgun sequence.
ACCESSION   DACEKQ010000242 DACEKQ010000000
VERSION     DACEKQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.268"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(269..568)
                     /locus_tag="IW671_002910"
     CDS             complement(269..568)
                     /locus_tag="IW671_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0899835.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          250 a          127 c           77 g          288 t
ORIGIN      
        1 ttcatttttt caatctatat ttccaatttt taacttacaa aaatgcaaca ttaataaata
       61 acgatagttt cataaaatac acttttaatt aacattatta gtttatacta gctgtttttg
      121 tgttaattaa aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa
      181 aaatacagac tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt
      241 ttaattcatt gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag
      301 ccattcctcg acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg
      361 gtgatactgg tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat
      421 tatttatgac tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa
      481 gtatacgttc cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca
      541 tgttttctat ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc
      601 actacattct ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt
      661 gttaaaacct ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt
      721 aaatcagtaa aaatttttat tc
//
LOCUS       DACEKQ010000243          734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.269, whole genome shotgun sequence.
ACCESSION   DACEKQ010000243 DACEKQ010000000
VERSION     DACEKQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.269"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..240)
                     /locus_tag="IW671_002911"
                     /pseudo
     CDS             complement(52..240)
                     /locus_tag="IW671_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            220..>734
                     /locus_tag="IW671_002912"
     CDS             220..>734
                     /locus_tag="IW671_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0899836.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT          255 a          114 c          162 g          203 t
ORIGIN      
        1 ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt tctaaaagtt
       61 cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt ttgtgagtat
      121 ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa gaacgcatag
      181 gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat taacagagta
      241 acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga aattgcacag
      301 cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga agaagccgga
      361 ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt cctacttccg
      421 ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa atttttaaat
      481 tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa aatcaaatct
      541 attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat taacgtgtac
      601 agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat tcagactgca
      661 atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca agaaccggaa
      721 agcagaatga taga
//
LOCUS       DACEKQ010000244          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.270, whole genome shotgun sequence.
ACCESSION   DACEKQ010000244 DACEKQ010000000
VERSION     DACEKQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.270"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..731
                     /locus_tag="IW671_002913"
                     /pseudo
     CDS             55..731
                     /locus_tag="IW671_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          125 c          137 g          172 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt c
//
LOCUS       DACEKQ010000245          711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.271, whole genome shotgun sequence.
ACCESSION   DACEKQ010000245 DACEKQ010000000
VERSION     DACEKQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.271"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>711)
                     /locus_tag="IW671_002914"
     rRNA            complement(<1..>711)
                     /locus_tag="IW671_002914"
                     /product="16S ribosomal RNA"
BASE COUNT          143 a          230 c          156 g          182 t
ORIGIN      
        1 gtttcaacct tgcggtcgta ctccccaggc ggagtgctta atgcgttagc tgcagcactg
       61 aagggcggaa accctccaac acttagcact catcgtttac ggcgtggact accagggtat
      121 ctaatcctgt ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg
      181 ccttcgccac tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca
      241 ctctcctctt ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg
      301 gctttcacat cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca
      361 acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt
      421 tagataccgt caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt
      481 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg
      541 ccgaagattc cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg
      601 gccgatcacc ctctcaggtc ggctatgcat cgtggccttg gtgagccgtt acctcaccaa
      661 ctagctaatg caccgcgggt ccatccatca gcgacacccg aaagcgcctt t
//
LOCUS       DACEKQ010000246          698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.273, whole genome shotgun sequence.
ACCESSION   DACEKQ010000246 DACEKQ010000000
VERSION     DACEKQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.273"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..>698
                     /locus_tag="IW671_002915"
     CDS             4..>698
                     /locus_tag="IW671_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899837.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDH"
BASE COUNT          305 a          110 c          152 g          131 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcattt
//
LOCUS       DACEKQ010000247          698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.274, whole genome shotgun sequence.
ACCESSION   DACEKQ010000247 DACEKQ010000000
VERSION     DACEKQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.274"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..347
                     /locus_tag="IW671_002916"
     CDS             15..347
                     /locus_tag="IW671_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0899838.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          249 a          100 c          152 g          197 t
ORIGIN      
        1 gggtggatta ttttatgtca tatacaggaa ttttgagtct cgaagatatt tgtcactacg
       61 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagtaaaa
      121 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag gaaatgattt
      181 attacgctgg taaacaaaaa caaataattt taaaagatcc tatcccttta aaagaactat
      241 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
      301 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttaat cagctgaatt gatcattctc
      361 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaagaa
      421 cttagaatta ctagaaaaat aaggagtaga cttgatctat caacaattcc tttcgggggg
      481 tgacggggta ggtttttacg attggtaacg taaaaaattg gtcaatcgag taaatgccct
      541 tgtttggata gtggtgaaat gaatttgtgt aaataatatg cacagagtaa acaagaggga
      601 atttagaagt tagacaagtt agaatagcgc ctagattaat catgagcagc tactaaggga
      661 tctaggcttt gacgcaaact catggaataa ggagtagg
//
LOCUS       DACEKQ010000248          683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.275, whole genome shotgun sequence.
ACCESSION   DACEKQ010000248 DACEKQ010000000
VERSION     DACEKQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.275"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..397
                     /locus_tag="IW671_002917"
     CDS             <1..397
                     /locus_tag="IW671_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899839.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            397..>683
                     /locus_tag="IW671_002918"
     CDS             397..>683
                     /locus_tag="IW671_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899840.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNG"
BASE COUNT          240 a          109 c          160 g          174 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgtagaag ctattgatgg taaagaagca
      481 ttggtgcaga cagatggaga ccaagccatt gcaggacata agaatttttc aggttctgta
      541 acaatcaata ataaacgtgt tttgacgact gatgataaca gatatgaagt ggtaaatctg
      601 gttgtcacta acggtaatac agggacagca aagctttatc gtgaaggaaa aacagtttct
      661 atatattttg tggctttaaa cgg
//
LOCUS       DACEKQ010000249          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.276, whole genome shotgun sequence.
ACCESSION   DACEKQ010000249 DACEKQ010000000
VERSION     DACEKQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.276"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACEKQ010000250          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.277, whole genome shotgun sequence.
ACCESSION   DACEKQ010000250 DACEKQ010000000
VERSION     DACEKQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.277"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..610
                     /locus_tag="IW671_002919"
     CDS             179..610
                     /locus_tag="IW671_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899841.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          260 a          100 c          135 g          173 t
ORIGIN      
        1 gaaggctatc ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact
       61 ttgaacagtg ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca
      121 agttatggat cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat
      181 gaaagtagtt tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca
      241 agaaaatatc cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag
      301 atttgatttt gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa
      361 tgctgttgat agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc
      421 agaaaatgat gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa
      481 gcaagaaaat gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac
      541 gatgcttgct tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg
      601 tgggaagtaa catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca
      661 ctaacgaa
//
LOCUS       DACEKQ010000251          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.278, whole genome shotgun sequence.
ACCESSION   DACEKQ010000251 DACEKQ010000000
VERSION     DACEKQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.278"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>665
                     /locus_tag="IW671_002920"
     CDS             <1..>665
                     /locus_tag="IW671_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ0899842.1"
                     /translation="VLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGI
                     IDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEG
                     TLR"
BASE COUNT          229 a          122 c          116 g          198 t
ORIGIN      
        1 cgtcttattt cctaatggtg actttcctag taggagtcga ttcactcgct taagttcaaa
       61 cttagcttac actatcaaga ttattcgata ctttttcatc aagaagctca ccaaaggtga
      121 actagtcgga attatagata gttttcctag tccattatgt aaaccagtta gaaatagaca
      181 agcaaaacta ttgaatcaaa tagccaaagt tggctataac tcaacaaaaa aatcttattt
      241 ttatggtctt aaaattcata tgatcgttac taagactggc tttccgatta cttactcaat
      301 cacgaatcca ggtgtccatg acgtgaaagt gttagagact ttatcggaag aagcgaatct
      361 tcccaatatc ctaggggaca aaggatatat tagccataaa atccatgaaa agcttgccct
      421 taagggcatt actatatccg ttccgccacg taaaaacatg gataaatcag aaaaactaga
      481 ccacagctta cttggaaaac aaagaaaaac agttgagact gttttttctt ccttagaaaa
      541 attaggttgt caaaatttca actcacgttc tgttaaaggg ctagagagta gatttgaaag
      601 catattactg gcttacagtg ttctattaag tcgagcacaa cgacgttttg aaggaacttt
      661 gagat
//
LOCUS       DACEKQ010000252          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.279, whole genome shotgun sequence.
ACCESSION   DACEKQ010000252 DACEKQ010000000
VERSION     DACEKQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.279"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..665
                     /locus_tag="IW671_002921"
                     /pseudo
     CDS             233..665
                     /locus_tag="IW671_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          242 a           78 c          104 g          241 t
ORIGIN      
        1 ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa agaggaagtc
       61 caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata tacatccctg
      121 ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga atgggataaa
      181 atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga tagtgaacat
      241 acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta ctttcaaaag
      301 attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat acaagtttaa
      361 ttttgtttaa tcttcagtat ctactgaaag tatctatttt tcaattcttt ctgagagtag
      421 gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata tatatacgtt
      481 ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg aatatctcaa
      541 ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac catatttgaa
      601 tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta atcaaggaaa
      661 tatga
//
LOCUS       DACEKQ010000253          650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.281, whole genome shotgun sequence.
ACCESSION   DACEKQ010000253 DACEKQ010000000
VERSION     DACEKQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.281"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>650
                     /locus_tag="IW671_002922"
     CDS             <1..>650
                     /locus_tag="IW671_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0899843.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          112 c          134 g          191 t
ORIGIN      
        1 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
       61 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
      121 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
      181 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
      241 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
      301 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
      361 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
      421 tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac
      481 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc
      541 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg
      601 attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag
//
LOCUS       DACEKQ010000254          613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.283, whole genome shotgun sequence.
ACCESSION   DACEKQ010000254 DACEKQ010000000
VERSION     DACEKQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.283"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          225 a           86 c          131 g          171 t
ORIGIN      
        1 atcacgaaga aactatcaac tggccaaaat aaaaccgttg tgcagtgtaa gaaatatata
       61 atccaaaaag acaaagtctc agaggaaatg atttattacg ttgggaaaca aaagcaaata
      121 attttaaaag atcctatccc tttaaaagag ctatatccaa caatcaaaca tgtttatgac
      181 caaaacggtg tattgatagg tcgaagaaaa aatggagtct tacgttgtac cgcaaaaggg
      241 atggggcgtt tgatcagctg aattgatcat tcttataact tgtattttat cgtttttcgc
      301 ttgcttgaaa atattgtcga gacttctaaa agaacttaga atcactagaa aaataaggag
      361 tagacttgat ctatcaacaa ttcccttcgg ggagtgatgg ggtaggtttt tacgattggt
      421 aacgtaaaaa attggtcaat cgagtaaatg cccttgtttg gatagtggtg aaatgaattt
      481 gtgtaaataa tatgcacaga gtaaacaaga gggaatttag aagttagaca agttagaata
      541 gcgcctagat taatcatgag cagctactaa gggatctagg ctttgacgca aactcatgga
      601 ataaggagta ggt
//
LOCUS       DACEKQ010000255          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.284, whole genome shotgun sequence.
ACCESSION   DACEKQ010000255 DACEKQ010000000
VERSION     DACEKQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.284"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW671_002923"
     CDS             complement(318..581)
                     /locus_tag="IW671_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0899844.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEKQ010000256          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.285, whole genome shotgun sequence.
ACCESSION   DACEKQ010000256 DACEKQ010000000
VERSION     DACEKQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.285"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW671_002924"
     CDS             <1..300
                     /locus_tag="IW671_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0899845.1"
                     /translation="FMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          208 a          101 c           69 g          203 t
ORIGIN      
        1 tttatgacta ggaaatggaa aaagcagtat ccaagagtcg tcaatttact cttgaatcct
       61 gccctattaa ccttttataa tttccctcac gccatcagac gaacaattta ttcgacgaac
      121 ctgattgaag gctttaataa gcagctaaaa cgatatactc gaagaaaaga acaattccct
      181 aatgaagaat ctctagagag attcttcatt tctcaattta atcaatataa ccaaaaattt
      241 ttaggtagaa ttcacaaggg atttaaagaa attcaggata cattagagtc gatgatttaa
      301 ctgtaattaa cagaatggat tttccattta catataattc ttgacgctac cataatctaa
      361 ataaccatca tattcatcta ctacttatat aattgagttt tattaaactt tttaagtttc
      421 tattctatca aatatttaaa caatctctgc atccgaaaag actacctcgt atagttttac
      481 taacaatatt tttattagcg tgttcaaatc ctgatttctt gtatcaatta ttttctgttc
      541 actttcaata agaaaaaagt ctttacttcc atggataata a
//
LOCUS       DACEKQ010000257          566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.denovo.287, whole genome shotgun sequence.
ACCESSION   DACEKQ010000257 DACEKQ010000000
VERSION     DACEKQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.denovo.287"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IW671_002925"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IW671_002925"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IW671_002926"
     tRNA            203..276
                     /locus_tag="IW671_002926"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IW671_002927"
     tRNA            316..405
                     /locus_tag="IW671_002927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IW671_002928"
     tRNA            420..491
                     /locus_tag="IW671_002928"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
BASE COUNT          147 a          120 c          150 g          149 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatc
//
LOCUS       DACEKQ010000258        33813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.021, whole genome shotgun sequence.
ACCESSION   DACEKQ010000258 DACEKQ010000000
VERSION     DACEKQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.021"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            159..1562
                     /locus_tag="IW671_002929"
     CDS             159..1562
                     /locus_tag="IW671_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0899846.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            1744..2928
                     /locus_tag="IW671_002930"
     CDS             1744..2928
                     /locus_tag="IW671_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0899847.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            2925..3215
                     /locus_tag="IW671_002931"
     CDS             2925..3215
                     /locus_tag="IW671_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899848.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            3212..3433
                     /locus_tag="IW671_002932"
     CDS             3212..3433
                     /locus_tag="IW671_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899849.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            3475..4254
                     /locus_tag="IW671_002933"
     CDS             3475..4254
                     /locus_tag="IW671_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0899850.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            4321..4815
                     /locus_tag="IW671_002934"
     CDS             4321..4815
                     /locus_tag="IW671_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0899851.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            4871..5509
                     /locus_tag="IW671_002935"
     CDS             4871..5509
                     /locus_tag="IW671_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899852.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            5581..5976
                     /locus_tag="IW671_002936"
     CDS             5581..5976
                     /locus_tag="IW671_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0899853.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            5960..8413
                     /locus_tag="IW671_002937"
     CDS             5960..8413
                     /locus_tag="IW671_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0899854.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            8410..10437
                     /locus_tag="IW671_002938"
     CDS             8410..10437
                     /locus_tag="IW671_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899855.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            10434..11456
                     /locus_tag="IW671_002939"
     CDS             10434..11456
                     /locus_tag="IW671_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0899856.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            11473..12402
                     /locus_tag="IW671_002940"
     CDS             11473..12402
                     /locus_tag="IW671_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0899857.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            12647..12763
                     /locus_tag="IW671_002941"
     CDS             12647..12763
                     /locus_tag="IW671_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0899858.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12779..14698
                     /gene="tet(M)"
                     /locus_tag="IW671_002942"
     CDS             12779..14698
                     /gene="tet(M)"
                     /locus_tag="IW671_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0899859.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            14796..14984
                     /locus_tag="IW671_002943"
     CDS             14796..14984
                     /locus_tag="IW671_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0899860.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(15042..15395)
                     /locus_tag="IW671_002944"
     CDS             complement(15042..15395)
                     /locus_tag="IW671_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899861.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15926..16348
                     /locus_tag="IW671_002945"
     CDS             15926..16348
                     /locus_tag="IW671_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0899862.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            16345..16575
                     /locus_tag="IW671_002946"
     CDS             16345..16575
                     /locus_tag="IW671_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899863.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            17073..17273
                     /locus_tag="IW671_002947"
     CDS             17073..17273
                     /locus_tag="IW671_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0899864.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            17300..18493
                     /locus_tag="IW671_002948"
     CDS             17300..18493
                     /locus_tag="IW671_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0899865.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(18561..20126)
                     /gene="guaA"
                     /locus_tag="IW671_002949"
     CDS             complement(18561..20126)
                     /gene="guaA"
                     /locus_tag="IW671_002949"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0899866.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(20185..21108)
                     /locus_tag="IW671_002950"
     CDS             complement(20185..21108)
                     /locus_tag="IW671_002950"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0899867.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            21304..21909
                     /locus_tag="IW671_002951"
     CDS             21304..21909
                     /locus_tag="IW671_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0899868.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            22217..23233
                     /locus_tag="IW671_002952"
     CDS             22217..23233
                     /locus_tag="IW671_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0899869.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            23374..24675
                     /locus_tag="IW671_002953"
     CDS             23374..24675
                     /locus_tag="IW671_002953"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0899870.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            24721..25383
                     /gene="deoC"
                     /locus_tag="IW671_002954"
     CDS             24721..25383
                     /gene="deoC"
                     /locus_tag="IW671_002954"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0899871.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            25404..25802
                     /locus_tag="IW671_002955"
     CDS             25404..25802
                     /locus_tag="IW671_002955"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0899872.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            25868..26950
                     /locus_tag="IW671_002956"
     CDS             25868..26950
                     /locus_tag="IW671_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0899873.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            27162..28724
                     /locus_tag="IW671_002957"
     CDS             27162..28724
                     /locus_tag="IW671_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0899874.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            28717..29850
                     /locus_tag="IW671_002958"
     CDS             28717..29850
                     /locus_tag="IW671_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899875.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            29850..30806
                     /locus_tag="IW671_002959"
     CDS             29850..30806
                     /locus_tag="IW671_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0899876.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            30905..32071
                     /gene="deoB"
                     /locus_tag="IW671_002960"
     CDS             30905..32071
                     /gene="deoB"
                     /locus_tag="IW671_002960"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0899877.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            32095..32913
                     /locus_tag="IW671_002961"
     CDS             32095..32913
                     /locus_tag="IW671_002961"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0899878.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            32930..33637
                     /gene="deoD"
                     /locus_tag="IW671_002962"
     CDS             32930..33637
                     /gene="deoD"
                     /locus_tag="IW671_002962"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0899879.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11073 a         5888 c         7050 g         9802 t
ORIGIN      
        1 ctctctatcg acgtcattat tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg
       61 cttatcagaa atatgaagcc atttctcaac caacttttga taaacaggct cgtatctaca
      121 atacacaatt attttgatag gtggaaagga ggatttttat gccattgctt catgacagag
      181 gagagcgaat caaagccaac gataagcact tggtctatca ttttgtacta atttcattgc
      241 tggtagtttt tctagtcgtc ctactccctt ttcattggag agcgttacaa tcggtgaact
      301 ggcaagcagt tcagttagat acatttttct atcaacttca tacgccatac ttttggagca
      361 gtattctcac tgctcttttt gtatgcggac ttatcagctg gctttattat cactatcgta
      421 tagattctat aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc
      481 attggtatga agccgaagaa actcaatcag agggattctt taaagattta cccagcaaga
      541 aaaccaaaca acgaatccgc cattttccta aaatatatta tcgaatgaaa gatggactaa
      601 tccatattca tgtagaaatc accttaggga aatatcaaga tcagcttttg cacctagaaa
      661 acaaactaga aacaggattg tattgtgaat tagtggaaaa aacgttgaaa gattcttatg
      721 tagagtatgt cctactttat gatactattg gcaatcgaat tggcattgaa gacgtggtag
      781 ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg ggagttcgat tctctccctc
      841 acatgttaat tgctgggggc actggtagtg gaaaatccta ttttattctc acattgattg
      901 aatctctcct gcatacaaat gcaaagcttt atattcttga ccctaaaaat gctgatttgg
      961 cagacttagg ggcggtcatg aataatgtct attaccgtaa agaagatatg ttggcatgta
     1021 tcaatcaatt ttacgaagat atgattgatc gcagtgaaac catgaaacaa caccctaact
     1081 ataaaacagg agaaaattat gcttatcttg gtttgtctgc caattttctt atttttgatg
     1141 aatatgtggc aatgatggat atgctaggtc gtgaaagtac caccgttatt cataaactca
     1201 aacaaattgt aatgttagga cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc
     1261 cagacgcaaa atattttagt gatgggattc gggacaactt taacttccgt gtggctttgg
     1321 gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag cgatacacaa aaacaatttt
     1381 tcttaaaacc catcaaggga agaggttacg tggatacagg aaaaagtgtg attagtgagt
     1441 tttatacacc ccttgtacca aaaaggtacg atttcttagg agaaatagga aaagtcattc
     1501 aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg tacaggttca gacttgcttt
     1561 gattggtgtg gcgttagcca cgccaatcct ttagccccct ctatctaaca ggggggcaca
     1621 aatacaactg aaaacaggca attctcatca accaatgttg atggtacaaa ggattgcgga
     1681 aaatatgaaa ggtgtaaact caagggtcat agtttacatc ttttttagat tggaggtttg
     1741 cgaatggaaa acgaaaaaat ttggattaag gaattgaaag aaaagcgact ggcttatggc
     1801 gtttctcaaa ataaactggc ggttgcttct catattacca gaccttacct atcggatatt
     1861 gaaacaggca aggcagtccc tactcaacag gtgaaagaag atttactgaa cgctttggaa
     1921 cgcttcaatc ccgataaccc attggagatg ttattcgact atgtacggat tcgattccct
     1981 accaatgatg tggctcaaat tatcggtgag gttttacgtt tgaatatgga ttatatgcta
     2041 cacgaagact ttggctatta ttcttatccc gaacactatc gtttcggtga tattgttgtc
     2101 ctagtctctc atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg
     2161 caatttgaaa actttctatt ggctcaacat cggagctggt atgatttctt tcaagattgc
     2221 atggaacata aaggtatttt caaacgcttg gatttagcta tcaacgataa aacagggatt
     2281 ttgaatattc cagatttggc aaagaaatgt aaacaggaag aatgcatttc cgtctttcga
     2341 agctttaaaa attatcgcag tggtgaattg gttcaccgtg atgaaaaacc atacatggga
     2401 aacacacttt atattggctc attaaaaagt gaagtctatt tctgtatata tgagaaagat
     2461 tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa gaatcggttt
     2521 gaaattcggt taaaaaatga ccgagccacc caagccatga aagatttatt ggcacatcaa
     2581 caggcggaaa aaacagcctt tgaaattatc aatcgctata tccgctttgc agataaagac
     2641 gacactaaac gtcggagtga ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa
     2701 aatcgtggag cattacgcct aaccacacag ccagagcctt attcttttga acgaacattg
     2761 aactggttac atcatcaagt cgcccctaca ttaaaaatcg caagcatttt agatgttctc
     2821 aacggtacaa ccattatctc caccatgatt caggaagcca aactgacaga aaaacatgag
     2881 aaattaattg aacaacaaca tctagccatg gaggatttga ttacatgagc cttttaacaa
     2941 aagaattaaa gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat atacaaaata
     3001 cagggaaata tacgtctctc attattgagg gaaaacggca agctggtgat accatctata
     3061 cctatgattt ttacaaggtg agattttata ataattatac caatcgagta accgtctatg
     3121 gagagcattt aacccctttt caattattaa ggcgtgtaaa aagctatatc tattatcgag
     3181 aaaaatatct taaggaaagg aggacaatca catgaatttt gggcagaatt tgtacaactg
     3241 gttcttggat aatgcacagt cattggtatt gttagcgatt gttgtcattg gactgttttt
     3301 aggttttaaa cgagaatttt caaaactcat tggcttcttg attattgctc taatcgccgt
     3361 cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat
     3421 cattggtgct taacagatat ttttcatgat aaaataggaa gtatatattt tgttatgaaa
     3481 ggatttatga atatgtttac aaatgatcaa tttaaatctc ttttaattaa aacattagaa
     3541 agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata
     3601 tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata taaaaagggc
     3661 tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt agactttttt
     3721 gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa acaaggtgat
     3781 aatgtattag tggatactat tttagtacca cttccagaga attacgaagt aattaacttt
     3841 agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga gtcttttatg
     3901 agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca tggatattta
     3961 attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct atctaaatta
     4021 tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact tgctgatata
     4081 gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga agcaagaaat
     4141 aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc aaaactaata
     4201 ataggtattt caagtacagt tacagattat attagcggat accttgataa gtagcgtcat
     4261 taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata agaattggaa
     4321 atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt cggagcatgg
     4381 tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac
     4441 gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata cacgccgatt
     4501 agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc tatttataat
     4561 gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact ggaaaacaaa
     4621 gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg ttattacgtg
     4681 gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat tgactaccaa
     4741 tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca tggggtattt
     4801 gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat agcacggaga
     4861 ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag acatgaaaga
     4921 taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt atattgtact
     4981 tgttaagcta tttgaaaaag aggaaaaagt tccagaatat gctctgttaa aactagaatt
     5041 tttaaaggaa aacgattttt cagatatgct agctgtctat gctaattcgg taaaattata
     5101 tacagacacg aaaacaataa gagaatattt tggtatcgaa tacagtagta acttgggaga
     5161 tgttttattt caatttagcc aaactcttgc tcgatttatt ccgacagaag ttagtgagaa
     5221 gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta agtcaaagtg attcagaaga
     5281 tcccagaaaa aaatattgtt tttcagttag aagaaatcct ttaaagggaa atggtgagct
     5341 gggaaaacga agtcctttta atgacaataa aacgagattg tatagaccca gcttatatga
     5401 acgattaggg tcagatacca atttaagttt cagatattca atgaatccag atgatgaaga
     5461 aactgatgaa ggtattattg caaaatggac aaaaaataaa attgaatagt taaagcattg
     5521 aaagagcaat cacctatttt gattgttctt tttctatttt tacggaaagg acgtgaagac
     5581 atgaaaaaaa taagaagtta caccagtatt tggtcagtag aaaaagtgat ttatgcgatt
     5641 aatgatttac aactcccttt cccaattacc tttacgcaaa tgacatggtt tatcgtttct
     5701 ttgtttgtcg tcatactctt tggcagttta ccaccacttt ctctgattga cggagcacta
     5761 ctcaaatact tagggattcc cattgttctc actgggttca tgtgccaaaa gacctttgac
     5821 ggaaagaaac cctatggatt tctaaagtct atcattacct atgctctacg ccctaaagta
     5881 acctacgctg gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc gattacgatt
     5941 gtaaggagtg aacaactaga tggcatatcc gattaaatat atggaagata atctcgtatt
     6001 taatcatgat ggcgaagtat tcgcttatta tgaattaatt ccctataatt acagtttttt
     6061 aagttccgat gaaaaaattc aagttcatga taatttctgt caattgattg cccaaaatcg
     6121 tgatggcaag attcatgctc tgcaactagc aaccgaatca agtattcgag ccacacagga
     6181 acaatcgaaa aaagaaatca ctggacgatt aaaagagata gcttacgaaa aaatagatga
     6241 acagaccgaa gcgttaattt ctatgattgg tgacaatcaa gtagactatc gcttttttat
     6301 tggttttaaa ctctacctca atgaacagga agtatccgta aaaaatgttg ggaaagaaat
     6361 tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac aaattaatgg gcgattttgt
     6421 ttctatgcct atggcggaaa tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa
     6481 attatccaga cgctttcaga ttcgtaaatt ggagaaagat gattttggtt atattattga
     6541 acatctttac gggcaaacag gtgtcccttt tgatgactac gactaccatt taccgaaacg
     6601 atacgaagat aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt
     6661 aatcgaagaa aatcaacgct atttacggat tgaacaggaa gataccacta cttatgtggc
     6721 gtattttaca attaatagta ttgttggtga attggatttc cctaatagtg aaatttttta
     6781 ttaccagcaa cagcagttta ctttccccgt tgataccagt atgaatgttg aaattgtcgc
     6841 caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag atttagatag
     6901 ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg gtggacgctt tagaatctgt
     6961 cgatgaatta gaaagtaatt tagaccaaac caaagaatct atgtacaaac taagctatgt
     7021 ggtacgggta tcagcaaaga atttggacga gctgaaacaa cgttgtgatg aagtaaaaga
     7081 tttttatgat gatctgtcta tcaaattagt acgacctttt ggcgatatgc taggcttgca
     7141 taatgaattt attccctcaa gcaagcgata tatcaatgac tatattcaat atgtcacgtc
     7201 ggattttttg gctggtcttg gtttcggtgc tacacaaatg attggtgaaa attctggtat
     7261 ttacgtcggt tacaatctcg atacaggcag aaatatttat ctccaaccta gccttgcaag
     7321 tcaaggaatt aaaggctctg taacaaatgc attagcgtcc gcttttattg gttcattagg
     7381 tggcggaaaa tcattctcta acaatatgct ggtctattat tcagtactct ttggcggaca
     7441 ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg aaagaaacct tacctgatat
     7501 tgcccatgaa atcaatattg ttaatctcac gagtgagaac gacaataaag ggttacttga
     7561 cccttatgtg attttaaaac tcccgaaaga ttcagaaagc cttgccattg atattctcac
     7621 gttcttaaca ggtatttcca gccgagatag cgagaagttt cctgtcctgc gtaaagctat
     7681 tcggagtgta actcaaagca aacaacgagg gttgttattg gtgattgatg aattgcgaaa
     7741 agaagatacg cccatcagca atagtattgc tgaccatatc gaaagctttg tcgattatga
     7801 ttttgcccat ctgttattca gtgatggcag tgtcacccaa tccattagct tggaaaaaca
     7861 actcaatatt attcaagtgg cagatttggt cttacccgat tccaatagct cttttgaaga
     7921 atacactacc atggaattat taagtgtggc aatgctgatt gtcattagta cctttgcttt
     7981 agactttatc cactctgacc gttccatttt taaaattgtt gaccttgatg aggcttggag
     8041 ttttttacaa gtggcacaag gaaaagctct atctatgcga ttggttcgag caggacgtgc
     8101 catgaatgca ggggtctatt tcgttactca aaacgcggac gatttactag atgaaaagat
     8161 gaagaataat attggcttga aatttgcctt tcgttccact gacctagtgg aaatcaaaaa
     8221 gaccttagaa ttttttggtg tggacaagga agatgaaaac aatcagaaac gcttacgtga
     8281 attagaaaat ggacaatgtt tagtttcaga catctatggt cgtgtagggg ttatgcagtt
     8341 tcacccgatt tttgaagagt tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga
     8401 ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact atagttggat aacgctcctt
     8461 tgtgtgggcg ttattttact tttattaggc acgctgggca atgcagtaca agcgacaggc
     8521 ttagttgatg aaaccattga tacaagcaat gaatactcca agtatggttt gaatcaatat
     8581 cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg gagtgatggc
     8641 ataggacaat cggtgatgta tggattgtat gccattacta attttatttg gacaatttct
     8701 ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt agatttcatt
     8761 agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat tacaccgaat
     8821 ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct cattgttgga
     8881 atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt
     8941 ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat
     9001 attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca
     9061 aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac
     9121 agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat
     9181 gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac
     9241 aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat
     9301 ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat
     9361 attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta
     9421 tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt
     9481 gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt
     9541 attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca
     9601 agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt
     9661 aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc
     9721 agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc
     9781 agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca
     9841 aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag
     9901 aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt
     9961 gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca
    10021 aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag
    10081 aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc
    10141 aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg
    10201 gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat
    10261 aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact
    10321 aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag
    10381 caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc
    10441 taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc
    10501 tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc
    10561 cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat
    10621 atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg
    10681 aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca
    10741 acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa
    10801 ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg
    10861 gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac
    10921 agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac
    10981 ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac
    11041 aatacctaac cgacacttcc ccaacagaat ttgatggtga aaccgtgcaa gtcattatgg
    11101 acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct
    11161 cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc
    11221 gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag
    11281 gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga
    11341 tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa
    11401 gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa
    11461 ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa
    11521 tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa
    11581 ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat ctataaaaat
    11641 tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca gcagattgtc
    11701 gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa
    11761 caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt gacggaagaa
    11821 ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc tagtgtgaac
    11881 gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt actgttttct
    11941 gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag ctttcatgtg
    12001 gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac aatgagtaag
    12061 aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac agtagataca
    12121 gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta tcctaccgcc
    12181 acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat taacaaggaa
    12241 tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca agtaatcgtg
    12301 aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc gcaatttgag
    12361 ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt ggtacatgat
    12421 tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc ctttacaaat
    12481 atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa
    12541 ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt
    12601 aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta
    12661 tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc ccagtgataa
    12721 aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat
    12781 gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta ccttaacaga
    12841 aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac
    12901 gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac
    12961 ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt
    13021 cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc
    13081 aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat
    13141 tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat caacggttta
    13201 tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta
    13261 tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg
    13321 aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga
    13381 acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc
    13441 aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat tttattcatc
    13501 aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa
    13561 aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt
    13621 tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga
    13681 attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt
    13741 gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa
    13801 tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt
    13861 gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc
    13921 tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc
    13981 actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat
    14041 ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt
    14101 ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga
    14161 gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat
    14221 acgctatggt tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt
    14281 taagtacggt ttatactata gccctgttag tactccagca gattttcgga tgcttactcc
    14341 tattgtactg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag
    14401 ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata
    14461 ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat
    14521 tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag
    14581 tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac
    14641 ccgtcgtcta aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg
    14701 cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata ttctttaata
    14761 tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag tggatattgt
    14821 gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata cttaaaaatt
    14881 tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa aaaatgaata
    14941 taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag aaatgaaact
    15001 ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc aatatttctt
    15061 gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc tgtaaagcta
    15121 tccatgtatt tttctacctg taaccttcga gtgcttttaa tcaaattatc tgtaggtaag
    15181 aaaaattcat caatagatac atggagtaat gatacaagat catacagcac ttgtgtactt
    15241 gggtgttgtc ctttgttttc tatatttgtt aaataacgtg ggtcaatttc aatcattgtt
    15301 cccacttgtt cacgagtgag accccgtttc attcgagcct ctttaatcgc taaaccaacg
    15361 gctctaaaat cgtatttatc ttctttttta cgcatactaa atcacctcta tacattttac
    15421 tgttcctata taaccataaa caggtataga aaaacatgtt atatagttta tgagttccta
    15481 tagaacactg tggaatttat taatgcgatg aatccaattt caaatagaaa ggtcgctgat
    15541 tttatttacc tatacaactt tataaaattt agtcatactc ctagaaagta gataaaattg
    15601 gcgacttaat ggagggaaaa gtcgccatga gaaagataat ctgtcatcta gttctcttaa
    15661 ttaagttctt atcaaaaaaa cttatccacc tgtactggat agatatgacc atcaatgtgg
    15721 ttttgcagat tacatagata gaaaaatcaa agcatatgaa cagagaaaac aatctgttta
    15781 tgtgcttttt tggtcactta ataaatatct tttaggattt ctcttcattt atgcaacaaa
    15841 tccccctttc gtgtcggggt ttagtatgaa gggagataaa gaagcacatg ccactccctt
    15901 tcacgacaga aagggggtga aaagcatgaa accatcttct tttcaagaaa ccatagaaaa
    15961 tcagtttgac tatatctgca aacaagttat tgaagatgaa cgaaaagatt acttcaaaca
    16021 tctagacaga ctatcagcaa aagaagtctc gttttctgat atagataact accttgtcaa
    16081 ctctttttct actgttgata cgtacgtcac tgatttccaa ctatttaaat tatttggatt
    16141 aagaatcaat gttgaaagtg atttattaag tgaagccctg cgtcatcttt cagagaaaaa
    16201 acggaacatt attttacttc attactttat ggatatgagt gatgtggaga ttgcggagtt
    16261 actaaatttg aatcgttcga ctgtttatcg tcatcgaata agtggactag ccatgattaa
    16321 agaattcatg aaggagtgta aagaatgaaa acagcttatc caagggttcc cttccccctc
    16381 attgtaaagg cgacagatgg cgacgtggaa gcaattaatc agattgttaa acattatcga
    16441 gggtatacct caaaacgttc attacgtcgc atgacagacg aatatggaaa ttctcatatg
    16501 gttattgatg aaaccctacg tggcagaatg gaaacgaaac tcatctctaa gattttatcg
    16561 ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc
    16621 gatttgggag gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt tttgataggc
    16681 taacaagcca aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa
    16741 aagagctgat gaactaacac gccatgacct acaaaattag tgagcgattc atgttagtaa
    16801 ttcgaaagta atctttagca ggattgctgc catgactttc ttatcgtgat aatgatactc
    16861 ccgtatagcc atagtccgag cgttagaagc gtcacaggca atgggtacgg ctacatgaga
    16921 accatgcaga agtgaaattc ctgtgagctt tgctagagct gttcgattgc taagatattc
    16981 ttctcttgtc agtgtattat tataaaacta tttgatataa ttataggtga atattggagt
    17041 tttcctagcc tataattggg aaaggagtaa taatgataac ggtcacaaaa aaggatttag
    17101 ttgagctagg atatggaact tctttttcag cagacatcat aaaaaaagca aaagaattga
    17161 tgattacaaa agggcatatc tactatcaat ctcgaaagct agatagagtc cctcgtgaag
    17221 ctgttgaaga aattctagga attcggtttt caaatgaaaa caggtctgag tagtttttgc
    17281 acagtaagga gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga atggaaccta
    17341 ttattttcgt gcaaatctag gttatgattc taaagggaaa aaaattcaaa aatatcgtag
    17401 tggattcact actaaaaaag aagcaagaga agaatattcg aaactgctat tgatgaaacc
    17461 agaagaatta agtgagaata aggataaaat gacgtttaaa cattatatct ttgaaatttt
    17521 ccttccatgg tataagacac aggtaaagct cagaacatat gaaaatcgct taccaactat
    17581 taagaagcat ttctcttatt ttgataaaat ggctgtttcc gatattgagc ctattgatgt
    17641 gcaaaattgg caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca
    17701 aggactattt tcagttgcta tggatcgagc aattgtcctt gggataacca ccacaaatcc
    17761 atctaagatt attggcaatg tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga
    17821 agaatttgaa aaagtaatct ctctaattta caaagaagat tactatcaac actttttatt
    17881 tatctctctg tggtttttat ttatgactgg aatgcgaatt ggtgaagcta ctgccattca
    17941 atgggaagat attgattttg actcaggagt actaaccatc aacaaaacac tctattacaa
    18001 gaatcaatcg aattatagat ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt
    18061 attagataaa tgcacattaa cttatttatc cgaatggaag gaagtacaac aaaatctaat
    18121 tcagacagac tttgtaatga gctataacgg tattcctacc caaaaacata cactatcata
    18181 tgctattgaa cgctatgcta agctggctgg tatccatcga atcagaatcc atgggttacg
    18241 gcattctcat gcttccttgt taatcagtat gggagaaaat ccacttatca ttaaagatcg
    18301 tctaggacac gaagatattg agacgacttt aggaacgtat ggacatcttt atcctaacag
    18361 taatttcgag gtagctaaca aattaaatgg agccatttcc tttaaagaag caaaacaaaa
    18421 ttatgatact tcaccaaaga atcaatttac tgtagactac ttgcgaaaca aagaaatgaa
    18481 aaaagtgcaa taaaagtgca gtaaaaagat aagaagagcc agaaaccctt ggtataagag
    18541 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
    18601 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    18661 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    18721 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    18781 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    18841 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    18901 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    18961 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    19021 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    19081 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    19141 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    19201 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    19261 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    19321 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    19381 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    19441 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    19501 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    19561 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    19621 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    19681 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    19741 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    19801 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    19861 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    19921 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    19981 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    20041 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    20101 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    20161 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    20221 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    20281 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    20341 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    20401 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    20461 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    20521 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    20581 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    20641 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    20701 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    20761 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    20821 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    20881 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    20941 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    21001 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    21061 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    21121 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    21181 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    21241 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    21301 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    21361 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    21421 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    21481 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    21541 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    21601 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    21661 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    21721 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    21781 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    21841 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    21901 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    21961 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    22021 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    22081 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    22141 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    22201 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    22261 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    22321 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    22381 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    22441 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    22501 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    22561 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    22621 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    22681 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    22741 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    22801 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    22861 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    22921 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    22981 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    23041 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    23101 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    23161 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    23221 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    23281 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    23341 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    23401 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    23461 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    23521 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    23581 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    23641 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    23701 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    23761 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    23821 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    23881 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    23941 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    24001 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    24061 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    24121 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    24181 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    24241 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    24301 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    24361 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    24421 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    24481 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    24541 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    24601 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    24661 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    24721 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    24781 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    24841 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    24901 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    24961 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    25021 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    25081 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    25141 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    25201 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    25261 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    25321 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    25381 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    25441 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    25501 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    25561 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    25621 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    25681 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    25741 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    25801 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    25861 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    25921 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    25981 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    26041 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    26101 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    26161 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    26221 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    26281 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    26341 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    26401 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    26461 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    26521 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    26581 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    26641 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    26701 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    26761 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    26821 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    26881 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    26941 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    27001 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    27061 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    27121 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    27181 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    27241 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    27301 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    27361 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    27421 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    27481 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    27541 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    27601 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    27661 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    27721 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    27781 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    27841 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    27901 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    27961 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    28021 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    28081 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    28141 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    28201 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    28261 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    28321 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    28381 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    28441 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    28501 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    28561 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    28621 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    28681 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    28741 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    28801 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    28861 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    28921 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    28981 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    29041 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    29101 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    29161 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    29221 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    29281 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    29341 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    29401 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    29461 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    29521 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    29581 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    29641 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    29701 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    29761 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    29821 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    29881 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    29941 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    30001 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    30061 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    30121 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    30181 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    30241 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    30301 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    30361 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    30421 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    30481 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    30541 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    30601 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    30661 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    30721 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    30781 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    30841 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    30901 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    30961 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    31021 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    31081 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    31141 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    31201 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    31261 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    31321 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    31381 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    31441 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    31501 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    31561 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    31621 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    31681 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    31741 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    31801 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    31861 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    31921 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    31981 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    32041 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    32101 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    32161 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    32221 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    32281 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    32341 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    32401 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    32461 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    32521 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    32581 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    32641 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    32701 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    32761 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    32821 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    32881 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    32941 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    33001 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    33061 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    33121 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    33181 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    33241 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    33301 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    33361 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    33421 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    33481 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    33541 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    33601 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    33661 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    33721 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    33781 ttaactctga gtcacaagga acaatttcaa aaa
//
LOCUS       DACEKQ010000259        26951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.033, whole genome shotgun sequence.
ACCESSION   DACEKQ010000259 DACEKQ010000000
VERSION     DACEKQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.033"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            366..929
                     /locus_tag="IW671_002963"
     CDS             366..929
                     /locus_tag="IW671_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899880.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            916..1800
                     /locus_tag="IW671_002964"
     CDS             916..1800
                     /locus_tag="IW671_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAQ0899881.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            2194..2691
                     /gene="dfrG"
                     /locus_tag="IW671_002965"
     CDS             2194..2691
                     /gene="dfrG"
                     /locus_tag="IW671_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0899882.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2763..4715)
                     /locus_tag="IW671_002966"
     CDS             complement(2763..4715)
                     /locus_tag="IW671_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0899883.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4722..5018)
                     /locus_tag="IW671_002967"
     CDS             complement(4722..5018)
                     /locus_tag="IW671_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899884.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            5584..5838
                     /locus_tag="IW671_002968"
     CDS             5584..5838
                     /locus_tag="IW671_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899885.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            6235..7836
                     /locus_tag="IW671_002969"
     CDS             6235..7836
                     /locus_tag="IW671_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0899886.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            8070..8369
                     /locus_tag="IW671_002970"
     CDS             8070..8369
                     /locus_tag="IW671_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0899887.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            8503..8880
                     /locus_tag="IW671_002971"
     CDS             8503..8880
                     /locus_tag="IW671_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAQ0899888.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            8929..11292
                     /locus_tag="IW671_002972"
     CDS             8929..11292
                     /locus_tag="IW671_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0899889.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            11610..12209
                     /locus_tag="IW671_002973"
     CDS             11610..12209
                     /locus_tag="IW671_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899890.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            12321..12545
                     /locus_tag="IW671_002974"
     CDS             12321..12545
                     /locus_tag="IW671_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899891.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            12897..13133
                     /locus_tag="IW671_002975"
     CDS             12897..13133
                     /locus_tag="IW671_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899892.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            13213..14067
                     /locus_tag="IW671_002976"
     CDS             13213..14067
                     /locus_tag="IW671_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0899893.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            14080..14517
                     /locus_tag="IW671_002977"
     CDS             14080..14517
                     /locus_tag="IW671_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899894.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            14837..15280
                     /locus_tag="IW671_002978"
     CDS             14837..15280
                     /locus_tag="IW671_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0899895.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            15425..15748
                     /locus_tag="IW671_002979"
     CDS             15425..15748
                     /locus_tag="IW671_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899896.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            16166..18976
                     /locus_tag="IW671_002980"
                     /pseudo
     CDS             16166..18976
                     /locus_tag="IW671_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19072..19827)
                     /locus_tag="IW671_002981"
     CDS             complement(19072..19827)
                     /locus_tag="IW671_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0899897.1"
                     /translation="MSNYYHLLNKLEDLNLKHTREILPEYLDTIVQENLSFVEALNHL
                     MTEEINSRNERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLRYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(19842..21068)
                     /locus_tag="IW671_002982"
     CDS             complement(19842..21068)
                     /locus_tag="IW671_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0899898.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIKADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVIFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYASVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTMTHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            21176..22003
                     /locus_tag="IW671_002983"
                     /pseudo
     CDS             21176..22003
                     /locus_tag="IW671_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22063..22425
                     /locus_tag="IW671_002984"
     CDS             22063..22425
                     /locus_tag="IW671_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899899.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            22659..23081
                     /locus_tag="IW671_002985"
     CDS             22659..23081
                     /locus_tag="IW671_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899900.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            23093..24508
                     /gene="cpaF"
                     /locus_tag="IW671_002986"
     CDS             23093..24508
                     /gene="cpaF"
                     /locus_tag="IW671_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0899901.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            24525..25340
                     /locus_tag="IW671_002987"
     CDS             24525..25340
                     /locus_tag="IW671_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899902.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            25340..26137
                     /locus_tag="IW671_002988"
     CDS             25340..26137
                     /locus_tag="IW671_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899903.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            26155..26517
                     /locus_tag="IW671_002989"
     CDS             26155..26517
                     /locus_tag="IW671_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAQ0899904.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            26689..26910
                     /locus_tag="IW671_002990"
     CDS             26689..26910
                     /locus_tag="IW671_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899905.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
BASE COUNT         9912 a         3516 c         4875 g         8648 t
ORIGIN      
        1 cattgtatag cattttacac aggagtctgg acttgactta gaattataat cacaaactta
       61 atagtaatta ctattgggat tacaacatta acaaataaag ctttaatatc aagtatgatt
      121 ttaatagtgt ttgattttat tatcatttat agtttgctgt ttttaataaa gaaagtaact
      181 gaatatttaa atccgttagg cacgaaataa attttaattg aggttgaatt atagcggtaa
      241 atcatacaaa atagaaatgt agtcttgtta agcgtcttga ctatctaatt ttgaatgagg
      301 agggttgtat atagaaaaag ggtagttaaa ttcgtagatc aaaaggctac gaaggagaat
      361 aaagaatgac agctatttat aaagcactaa aaaattctaa cgtatcagag tacgttaagt
      421 cgagagtatt aaataataat ttagattaca ttttagaaaa tggtgttgct gatttaatca
      481 gtaaattaag tgatcctata gatatagatc atttggtggt atggcaagat ggtactttag
      541 aagtatcaat atatggagaa ttttatagta gaaataaagt aactacattc aaaaatagta
      601 ttagtggtcg agaagattta aaaggaatca ataaaaatag taaattctat agaacaaata
      661 aagaattgct agaaaaaatt tcaaagcgtg tttttgaatt ggtgaatcca ttttcaaaaa
      721 aacgtagtaa agaacttttt ggaatttttt atgatggttt tagtgcaaaa agtaatgtag
      781 aaaaattttt ttattttaat gattcagctt atattgcggt taaagacagt aaaagaatcc
      841 ctgaagcaac tttagaatat ttagagccaa ttaaattgtc tgttttttat tcactttatg
      901 agaaagaaat cgctgatgaa gcggtttaag gggagtaaaa tgagtaaaaa aataggcaaa
      961 gagtcattat cgctcggatt gaaaaaagaa attgttaaat ttaaaagtat gtatagagag
     1021 ccggaattta gactcatttt tcaattagat atagtgagag ggctgaaaat tagttttagt
     1081 tgtttaggtt atctggtctg gctattcatt gctagagtag gatggagcaa tgcttatagc
     1141 ttttataaac tgttcgccgg aactgatcga ttaaaagaat cattagaggt gaaagaagcc
     1201 ttaaatattt taggaagtct tgctttcagc ctgtttattt ttgctttatt tatcttacaa
     1261 gtaggaaaaa taatgatgtc aacaaatgac aattatatta attatttgaa gccttttcaa
     1321 aaaggaataa aaatcatttg tagacagggc acagttttat ttctacttag cctgtatgag
     1381 catgaaattc attttcaaaa ttattataat acgacaacta aacttggtgc atttttactc
     1441 ctatatttag ttagtaatat agctgaaaaa atgctgttta aacatattgt aaagcaagtc
     1501 ttttctgata ttcaaaagga aagctattgt ttaaaaacac cttatggaaa tttaaaaaat
     1561 gttgagctgt ttttatttaa aatggatagt aagtgggata aatatatcta tagaagtaaa
     1621 gaagtatttt taactggtag ttataacaaa actcttcagg agagcctgta cgacacagga
     1681 gcaatattta ataatgaacc tatggttttg aaagatttct ttggagatta tgaactagtt
     1741 tggaatccaa aacagcttag tgaacagggg gcagaaaatg aatttgctga agttcactaa
     1801 catagtgtac agaaaaacat tatctgaaag aagaacgcct tcaaaagaaa gtacaagaac
     1861 gattgggccg tacagaagac taacgaaaga tcgtaaaaaa aaggaaagac aatgaaaatt
     1921 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
     1981 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
     2041 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
     2101 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
     2161 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
     2221 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
     2281 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
     2341 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
     2401 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
     2461 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
     2521 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
     2581 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
     2641 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
     2701 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
     2761 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     2821 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     2881 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     2941 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     3001 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     3061 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     3121 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     3181 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     3241 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     3301 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     3361 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     3421 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     3481 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     3541 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     3601 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     3661 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     3721 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     3781 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     3841 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     3901 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     3961 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     4021 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     4081 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     4141 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     4201 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     4261 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     4321 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     4381 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     4441 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     4501 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     4561 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     4621 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     4681 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     4741 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     4801 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     4861 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     4921 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     4981 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     5041 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     5101 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     5161 ttttcacttt ttgtactcca aaactgaact acttaaatga aaatgtcttt cctttttttt
     5221 tgactacaag tagcctgaaa atgaaatttc aaaagactac aagtagtctt ttgtggcaaa
     5281 aataaagagc caacgaaagc taccaacaat cgttgactcg taagactgtt cgtagtaaga
     5341 aaaatctttg gcaatccacg aagtctttat ttcataattt atataaatga ttatacagtc
     5401 actttcctga atttgcaagt aaaaaagcta agaaatattg tttaagcgtt tccttaaaag
     5461 aatctttaaa taaatttttt tccaattgaa aacatgacta caagtagact atttctttta
     5521 ttccaagttg actacaagta gtcaaggtga tataatatat atatcaagaa aggagaaaca
     5581 aaaatgtata acgtaggaga tcaaatagat gagatcgtaa aaataggtaa tgttaaactt
     5641 gttaaaagta agtactatat ttcagaaact gaaataattt ttaatactga atcaggatgt
     5701 attctaattt ggaaactgcc ttttcccaaa gacgaaacaa cactgaaaaa aataaatact
     5761 ttaaaaatca gatattgcat tcatgaaatt attgataaaa atacttatgt tataaatcaa
     5821 gtaaaagtga tggggtaaaa atcttttaaa aaaactcttg ttaaaagtaa ttctattagc
     5881 ttataatgtt ggtattagtt ctgaagccaa aggaacacta aaaaattcta gtagaaaagt
     5941 tagttacaga ctctctacta tgtatgaatg taacgaaaaa aatagcgaaa aagataatta
     6001 aataagacta ccaatcttat ttaatggaac ttctgtagta agaaaagcga tttggcaatc
     6061 cacgaagtcc accaagatga gctaccaact cattttaatt tttgcacgtt taaagtttag
     6121 catttaagtg cttactctaa agacatagtg taattttagg actccttatt acggaatcta
     6181 aagttaccct atgtcttttt tgctattcaa aattgatagc aaggagaaag tgcaatgttg
     6241 aatagttgcg agtatgcgga cgttttagct aaaattctta aaaatggaat taaacagttt
     6301 aatagcaaaa atctagttgc ttttaaaaag aatagcaata agattggtgc tattgccgga
     6361 tttacttcta aaacaaacat gataaagact aaaggtctta tatatcgttc tattgatcgt
     6421 gtcttggaag attcagatgt attaacacac tggactccta acgtgtattc atggcttggt
     6481 ggcggtgaaa acaaaacaat ttttggtcac aaagaagcta acttaattca gattaatact
     6541 tttgtagcag atattgacta tccagtagga gaaaagaaaa cggatttgaa tttactagtt
     6601 ctttatctat tggaagaagg gttattgcct acactgattg tagatacgcc aaaaggttat
     6661 cattgctatt ttctgattca aaactataac caagaaactg attcatttga taaggcaagc
     6721 tatatttcta attcaaatga ttttaaatct ttgagagttg ctaaaagaat atcagaaaat
     6781 atcagaaaat ctatccaaaa tcgtttacca caagtcgata tgggctgtaa tcattttggt
     6841 atttttagat ttccaactaa aaaaaatatt gtacattatg aacctaattt cgtagatacg
     6901 tttgaagggt atttgaaatg gtcaaaagac tttgaagaaa aagaatatgc tttaaagagg
     6961 tctaacttgt cattattagc tcctaattct actggcaaga aagggtctaa acgacaaatt
     7021 gatactaaat ggtacgactt tttaattcat acggatattg ctgaaggaga tagaaataaa
     7081 actattttta ccttagccct ggcatgtaaa tcaagtggct tatctattgg cgagtgtatg
     7141 gatcaattgg atcaacttca attcaataat gatttgtcct attctgaagt tgagagaaca
     7201 ataagaagtg cttatcgtgg cgattacaaa ggtgctaaaa gttcatatgt caatgaaatt
     7261 atcgaaaatt atgctacacc taccgagttt gaaaaatact caataaatgt aaatagacaa
     7321 gctgttaaac gtaaagggaa tcgtgtcgat ccgtctaatt gggtgaaatt tgctaaacct
     7381 agagaagaac gaaaatatag tcattttgaa gaaagtaaag ctgatttatt agcttactta
     7441 gaaagaaaac aaagatcatt gaaaaaaaat gagttgtttt taagtgtctc tatgggaact
     7501 atatctgaag aaacacaaat tcctgtatct acgttaaagg tgattttaaa agagcttaga
     7561 acggcgaata cagtgatatt acgaacgtct cgtggtcggt atggatcgac taaaatagca
     7621 acaagcaaat ttgtggaagc taaaatatta aatacagtta ttcagaataa aaaagataaa
     7681 caagtatttg tcttagaatt attaggaaca gcaaatagcc acgtagaaac gattttaaga
     7741 cgttatgaag acaaaacagt accgttccat gcgaacatac tgaaagaaag agaaaagagc
     7801 aaacatgcgc caattagagg agctaataca ggttagagtt ctatttattt ttttgatttt
     7861 taaatagcta aattgccaat taacatatct agtgtttctg cccactccta ttcctgtttt
     7921 agcgtatact aatgattagt agttagtact ctcttttatg tatggagtgt atagaactat
     7981 ttttacttgt gtcttttccc ttaatccacg tgtacggaat acagcttata actgcgtatg
     8041 cagttatgtt tgaaggagga agattttctt tggtaattaa gaaatatgtt tactcacacc
     8101 cgatcaatgt atttattatc ggtaagctag gtttatctgt tgaggtgttt tgtgagttat
     8161 atggatttaa acaaggaact ctatcgtctt ggattactag agagcgtgag gtagctgatt
     8221 tgcctatttc ttttttgtat gctttgtctc tagcttcagg agggaatatg aatgaggttt
     8281 atgattgctt gtgtgagtat gaaaaagagt ataaagagta tttgaaaatt tcagccccaa
     8341 aacggaataa aaaatatatt gaagattaga gaaagaagaa aagaataatt tgttttttga
     8401 ctacaagtag acagatagct actctgtttt ttgatagttt gatattggct gtttttttgt
     8461 tagaattaat atatatcagg taaataaaaa agggggctat taatgaatat tgatgggatt
     8521 tataatgaat cagattttaa aaaaggcata gtagatattg atagaaaaga gtatttattt
     8581 cctgatgaaa taggtttatt taaaattgta ggtgaaactg tctgtaggta ttgggggaag
     8641 aaaggaaata taattttact tgttgatacg attgagggaa gaaagttaaa attttcgttt
     8701 tggagtaata caaaatattg ttgtgaacct gtacctaaaa gggaagtaga tgtggctaaa
     8761 gaaagtgtac gatttttacc tgatcgatca aaaataactt taatttgtga acggacaagt
     8821 aatgctaaat ttacaagaat tagagatata ctactttcga ctacaagtag tcagtcatag
     8881 agtaagaact tttggttaaa aatcaattga agaaagtgga ggaagtatat ggcagtagtt
     8941 atattagcag aaaaaccaga tcaaggagca aaatttgctt cagcattggc cggaaaaaaa
     9001 ttatcgaaaa agagtgggaa gtatgaattt aaaagtgaaa tatttggaga tactattgtt
     9061 acttggggaa taggtcattt ggtaggatta tcattacctg aaaagtatga gtggctaccg
     9121 aataaagata aatggcattt agataatttg ccttttctac ctaaattcaa tgaattaaag
     9181 tatgaagtct ctaaaggaaa aaaccaacaa ttttcaatag tgaagtcatg tttagaaaat
     9241 gcagatgaga taataattgc tactgatccg gatagagaag gagaaaatat tgcttataat
     9301 attttcaaat tatgcaagca gtcgattttt aataaaccga taaaaagatt atggattaat
     9361 tctttggttc aaaaagaaat tatcaaagga tttaaaaatc tgcgaaattc tgaagaaacg
     9421 atctcttact tcaaagaagc taatgctaga caaattgctg actatctagt agggatgaat
     9481 tatacagagt tgtattcatt aaagttgcaa gaaaaaggta tatcgggtgt ggtatcactc
     9541 ggtcgagttc agacaccggt aaacacactt gtggttgaaa acgatttggc aattagaaat
     9601 tttagaccgg aaaaatataa aacgattgaa tgtcatacaa ccgataagca accaaaagca
     9661 atatttaaga atagtcttga atactttaaa ttagaaaaat ttgaagaaga cactaaaaaa
     9721 ttcggccttg attccgccag tactggaatc ataaaaaaaa ttgaggttag taaaaagcaa
     9781 acagaaccac aaaaattgtt tagcttgggt ggtattcaag aatacgctaa taaaaaatgg
     9841 aaatattctg ctaaaaaaac agataaaatt atccaaagtc tatatgatcg tggatattta
     9901 tcttatccaa gaaccgatag tgaattgata actacaaatg aatttgagta tttaaaagcg
     9961 aatttagacg tgtataagag tgttttaggg atcagtattg atacaccttg tttagagcct
    10021 aaaaagcggt ttgtagataa tgacaaagta ttagagcatt atgcactaat accaacaatg
    10081 gatattcctg atatagcaaa tctgacatta gatgaaaaaa atatttatga gatcgttact
    10141 aggcgtgcgt gtctaatgtt tgcagaacct tacaagtacg aaaatacaaa agttacatta
    10201 gatgtaaacg gcttagaatt taccacaagt ggtaatgtac ctatatcttt aggttggaag
    10261 caagtagata ctaatttaga tgaatctgat gataaagagg aaacattacc taaatttcat
    10321 gaacaagaga caataaatat agatattaga tttattgata aattaactaa ggaacctaaa
    10381 aggttgactg aaggtaaatt attaggtaaa accggtatta tggcgaaatt agggctagga
    10441 acgcccgcta cacgttctaa tattgtagac actcttctaa atagggaata tattaagata
    10501 gaaaacacga atgtttatcc tactccaaag ggatatttat tatgggattt gacaaagaat
    10561 aaagacctat tgattggaaa accagaaaat acagcgatat gggaagaatc acttgggaaa
    10621 attgcgcaag gtagctatac acaggaagct tttttaagaa atatctataa ctatatcaat
    10681 aagactgttt cagatttgaa aaaagtagaa tttacttcgg attatttaga aaagactgta
    10741 aatcaaaata aatttgatat aggtagttat caagtggaag aaaagaaaaa agtttattct
    10801 attttaaata ttactagtgg tgaacagttc gtgatattta aaaatatatc aggaaaagca
    10861 atcaataaaa aagttcttca agaactatta ctaaataatc gtacagataa agtcctttcc
    10921 ggctttaagt caaaagaagg aaaagctttt aatgcaagat tacttttcga tccaaatgtg
    10981 atgaaagtgg taatggagtt tgaaaagaac agtccaaaag gcgataaaaa gagcaaacac
    11041 gatacagtaa atggttatga aatagttgaa aaagcaaaag tatttgaaat aaaagaatta
    11101 gcaacaggtg aaagttttat tttctataaa aataatagtg ggaaaacttt aagttcaaaa
    11161 acagtcaaag aattgttaga aaatgggaaa actaaagaga aagtaacggg ttttataagt
    11221 aaagacaata aaaaatatag tgcttatctt gtctttgata ataaaaacaa agttataaag
    11281 aaagaatttt aattcttagt ggcggaaatc atttgttttt ccgcttattt ttattcaaaa
    11341 gtgcccaaaa tataggtata ctgaattaag gataaataca aaactagtta gaggacagtt
    11401 gaaaaaatac aaacaaatta gattgctatt ttgttaaatt ggaagtagaa taataccgtt
    11461 ttgatatgta tagtgtgttt taccaataaa attacataaa cgtgaatata tttttatgaa
    11521 ttaaatgtaa aatgagagca attagtttta aaaaagatta taggctgtta atcttagcat
    11581 atataatcca ttggaaaggg ggagatccaa tgaatctaaa gaatacaaac tcaaacaaat
    11641 ctacactata tgtaatttca gatgattata taaataagct gaaaaaggtg gactatcgtg
    11701 tccaaaacaa ctatcgagaa caacgattat attttaaatc aaacttgaaa atttctaaca
    11761 aaaatgaaga aataaattat tatgtaccat tggcttcagc aaaagaaaat caacgttctt
    11821 tgactaataa atcagtgttt aagatttatg gtgatcgaga agaaaaggac tttttaggag
    11881 tacttcatat aaataatatg attcctgtgc caaaagattg tgcaaaagta tggacaccta
    11941 atagcccaaa tgttgatgag aagtataaga tgttagtaat caaacaggca aaatatttga
    12001 aattacatga gtctgaaata aatgaatcat gcgaattact ttataattct aagttaggta
    12061 aacttgatcc tagttacttt aagaaaaatc gtaaagaagt aattacttat caaaaaataa
    12121 tgacgaatgt agaatcattg gaagctgtta gtatagcaga atcccaaaga aatattcatg
    12181 tacaaactca aagtatagat gtagagtaag ataggctaaa cctatcttat tttattattc
    12241 tattggatat tatgatacat tgaattttaa tcgaatatgt atttaaatgt tattatactg
    12301 tagaagataa aacttaaagg atgatttcta tggcgagacc gagaaaatac aatacagaag
    12361 aagaaagaaa agaagcaatg aaaaaggcta ggaaaaaatg ggttgaaaaa aatcaggaaa
    12421 ctattgccta tataagagcc aaatcatttg ctaaaaaatt cattgaaatg tctgaagatg
    12481 aagatattga attgttggaa aaatggttag aagatcggaa aaataacggt caaaatgatg
    12541 aataataacc attgactaca agtagtcaat ggtgtagaat ggttttaggg atagaaatat
    12601 cccttttcta aaagtctaat gactacaagt agtcgataga gggatcggga ataaaaacag
    12661 acgattatgg cctaaagaaa ggaggaatat gaatattttg aggaacgatt tattaaatga
    12721 gcgttgataa atttcactaa atattttctt aatatctatg tattgttcaa gcgtagaaaa
    12781 aaatattgaa attattacaa aaccgctcat aatggaaaat aacgaattat attaatgaat
    12841 cgttcacttt tgaccttttt tactagattt ttttgtagga cttttagaga atttttttgt
    12901 tatctgatta taaggaggag ctaaagttga tagagaatag agtactgggg ttcgtagagt
    12961 gtaaagataa tttagacgat actttagaag tccttgaaag tattatattt aacaccaaag
    13021 ttctggattt gaaagcaatt aatggtgata tgacaacaca tatcatatta gatgaaatta
    13081 gcgcaaaaga gattggcgaa actttaatag catgggctaa taaagagctg taaaagataa
    13141 ttttaaaata aagtggccaa ttgactacaa gtagtctgta tagtataata aattatcaaa
    13201 agtgaggtat acatgatgaa atcaaatttt ttgggatatg taattgcaat caacatgttt
    13261 aaagggggag ttggtaagtc aactcttact cagctaatgg ctgatattct atcggctaga
    13321 ggctttaaag ttttagttat tgatgttgat ccacaaggaa cagtaactaa aaagtttcaa
    13381 agacaatatg atttaaaagt tgttgggaaa atgacattgt ttgaagcagt aattaatatg
    13441 tctttcttag actgcatttc tcctgttaaa gaaaacttag atattgtgct tggttcagaa
    13501 caaatgtcaa ggtttaccaa agtattaaga gatatgtgga gaagtactgg ttttactgat
    13561 gaaggtgaag aagtcttttg gaatttgttt aattatatga tcggtaatga taatttaaga
    13621 gatcaatatg attttatttt gtttgacact attcctacag tttcagaatt tacagataac
    13681 gtttacgtgg cgagtgattt tttagtaatt cccacgcaaa ctgagcaaga ttctatagac
    13741 aacttgcata gtacggttac taattatcaa actgctgtaa aagaagtaaa cggagattta
    13801 gaaattttag gtattgttcc atatctagtg gatactaaat taaaaacagg attagaactt
    13861 ttatcagaac ttcaagaaga atatggagac ttagtttatt ctcatattgt ttcagaacga
    13921 ggcgtaatta aacgttgggg tagagaagga ctaaataaat ctatcccaca ttctaaaact
    13981 gcttatagca tgtatgtaga cattgtagaa gaaacgttag tacgtttaga aaaagctaca
    14041 ggagaaaata ttttagaacg tgtttaggaa aggtgttgca tgatgtcgaa aactaaaaaa
    14101 gtaactagtg ccggcagtaa tggttcgggt aatccgttcg gaataaaaag cggtagtggt
    14161 ggatttaata taggacaaca aatgttaaaa ggtaaagctg gcattaagtc tactggtgat
    14221 aaacggaaat ctgttagggt accagttgaa ttatacgatg aagttgttaa agatttatca
    14281 tacggggcta aatattcatt aatgacagaa attttagctt tatttttaaa agaatttgaa
    14341 acacatggaa atagtatagt tactgatttt ggcaagttga aaacgagacc agtaaaaatt
    14401 tctgaagaag cctttaaagt tttgaaagtt ataaaatttg aaacagatca atctaatact
    14461 aatattattg cttcagcttt gacatggtat cttgataaaa gaaaaggaaa agcataatga
    14521 aatgaggtaa gactatggat cataaatcaa attattcata caaagaaaat tatatttgga
    14581 agtatgtagt agccgttttt ctactggtag gtggaattat tgcattggtt aaatgggcta
    14641 taattcctaa tgctttagta tttatcaatt ggtttgtccc taatttttgg agtttttttg
    14701 gctatatatt gataatctgg tttatgttta aagtactctc ttttttctgg tcgcctgtat
    14761 attctttgct tggaaagtgg tcgtacctaa ttttaatagc gacagctatt tatatcatta
    14821 tgcagattaa ctaattatgc agattttaga agatattaaa atttattgga aaaggataat
    14881 aggctataca ttagttcttc ttgtagtaaa tttattcctt tcgatgatag cgttggtctt
    14941 tgttaatcaa agaaaagaca atgtaactga attagtaacg tttatttcgt ttttcataag
    15001 tgggattttt gctcctttct ttgaagaaac cttaatgaga ggaattatcc aaaaccaatt
    15061 ggaattaaaa acaaatttgt ctacaagaag catatatata attgttgcaa ttttgtttag
    15121 tctatcccac ttaaatttat attttattcc ttattttatg acgagtttaa ttttgtcttt
    15181 tgtctatgat aattcagata aaaggctgat tgttccggtt actattcatt gtttttacaa
    15241 tttaattgta atactgttaa gtgtagtttt ttatacttga aaaaaataga cgaaaaccta
    15301 aaaatacaag gtttttatct atttttttta ttttgttttt atctatcagg ttgatagaaa
    15361 ttgtcataaa tgatataatt aagtatgaac tgatttagta aaaaaattaa aggagtgata
    15421 gctgttggca agatcaagaa ttgctaaaat tgaacagact aaaaaggata aaaaaatttc
    15481 taaaaataaa acaaaattct ttaaagctga agatgttaag aggatagcat ggtctgtgaa
    15541 aacatttgat gaagatcgtt atgttaaagt ttcaatgaat ggtctatttg tacagcttgg
    15601 taacttcagt aattttaaaa cgattgatga actttatccg gaaataagaa atattgtgga
    15661 tagtaaaggt ttaatcgtgg ctaaacggaa atcaagaaac tctggtcttg cttactgtaa
    15721 aaataaaggg atcatgattc cggcttaaat cagtgaaatt gttctttgaa aacttaatac
    15781 tactcgtact gtagcaatgt tgctatgtta cagataaaag cgtaaggcta ataagcaaaa
    15841 tagcaaccaa gcatactgtt atttcctttg ccaagaggta tgtaaatacg ttagcaatag
    15901 aagtgataag gttagtattt cacgccaaaa aatacaaaag tcgatccatt gaaactacta
    15961 attatgaggt ggcgtaaatg gtaattattc agaatttcta tctaggaaga acctatgaaa
    16021 accgtcctag agatcagaat attgacacta aaaaattcag cacagtaata aatgtgctag
    16081 atacagggta actctttact acaagaaatg tagtaaagag ttgttatgat tctttattac
    16141 atttctttct aaaggaagtg tgataatgaa aaaactaaaa ctgattagca ttgctctatt
    16201 cagcctaatc actatgtcaa cggctcgcca atcaattgtt tcggctgaag gaacgtctac
    16261 gaaagcagta gtagaagagt ctggcgataa aaaagttgct atagacgaca aagtgaaaaa
    16321 cgaagcggaa aaagcgactg tggttcaagg ggcagacggc ttgatgtatg tggtcgttga
    16381 tgaaaatgat cctgaaaaat tgctagaagc tgaatctcct ttgaaaaatc aacaagaaaa
    16441 taccggcgct gttgaagtcg gtgttgatga aaagagacaa gaaaaggctg ataaagctaa
    16501 cgaaaaagtg aaaaactttg acgatattga ttacaacgat attagttcgt tgcagtcagc
    16561 gttgaatgat ccgaaattcc cttttttatc ggaagatgaa ttaaatgaaa aattgcaaaa
    16621 gacaaagaat acatttgagt ctgaagcgct aatgaaaaag acaaggggat atttcagtca
    16681 aaggatttta gagttaagca ataatttaaa tgacgtaccg aaaagagcga aagtgagtcg
    16741 gcatgcttta gttagtaacc gtgcttcaca agtaagtgaa acacgaataa accctccaaa
    16801 tggttatccg tgggtgattt atttaattag gagcaatggt tatagttata cagaaaatta
    16861 tgctattaga agaactattg atgggaaaga agctttttgt ttacaggccg gtgtacaagc
    16921 gacgaataca tacactggtt caacaagtgg ttatctttct aattcgcaaa gagaaatgat
    16981 tagttcgatt ttatcagcat ataaagaatt aggcggttgg gctaataatg atgccggaaa
    17041 acaaagatgg atagcaactc aaatgcttgt ttgggaaaac agtaatgaaa agcctaaaag
    17101 tataactgtt acgccaaatt ggaaaaacgg agctactaag tggaaaaatg atttagtggc
    17161 tcgtgcaaat gctattagag taagaacaaa atgggattct acgtctaaga cggttgatga
    17221 gggagataaa gcaaccttta aagtaactaa tacttatgga gatcataatt tgcatgtgaa
    17281 atctgtatct ggtggcggca aagctaccat aagcaatggt actttaactg ttgatactac
    17341 aggagccact ggaaacaaaa tcactgtaga tatgattaaa ggttctcacc cttcagacgc
    17401 gcaccctaca acggtatgga gtaatggttc ttatcaaaag cttgttaccg gttcttttta
    17461 cattagtact tctgttacgg taaatgttaa gagaaaagga actgttgaag ttttaaaagt
    17521 tgattcagaa acaaaaaaac cattatcagg agcaacattt agatttagct actcaggtaa
    17581 aacaaaagat gttactactg attcgaatgg taaagcaaaa ttaactgaaa ggttgaaagc
    17641 tggaactgta gtaaaagtta aggagataaa agctcctaat ggctaccaac tagattcaag
    17701 cgaattttca attacagtta aagaaaatca aaatgttact accacaagaa ccaataagaa
    17761 atccacaggt tctgtagaaa ttgaaaagat aggcgattta gatggattag gactaccgaa
    17821 tgtagtattc acgatttata attccgataa caaagttgta aaagataact taaaaacaga
    17881 tagtaatggg aaaataaaag tcgatgaact gcaatttggt aaatattatg cagtagaaaa
    17941 acaaggtgtt actggttatg atcccgatgg taaaaaatac aattttgaaa taagtcaaga
    18001 tactcctttt acaaaaccgg ctaaagtgaa agtaacaaat atttatgaag tatccataag
    18061 cgaaaaagta tcggacatga atgaaagtca ggtattaaaa aataattggt ctgctgatcc
    18121 aaacgattat cttcaatatg acattacaac tggaaatcta aaaaaatcgg gagaaagaaa
    18181 cgtccaaagt ttcattatcg aagggtttta cgacaataag aatgtagatg tgtcaaaaag
    18241 tgaagtatat gttggatcaa caaatgtaac aaactctttt gacattaata acgatactaa
    18301 caatggaaaa gtaattgcta aggcaaagac aagcgtatta acgaataatg atttttataa
    18361 taagtcctat aatttacgga tcactatgaa aattaaaaaa tctactcttg aaaacactga
    18421 aaagcaaaaa aatttaacga ttgttaaaaa tagtaaagtt actgtcggtt caaaaatctc
    18481 taaatctgct agctctaatg atgtagaaac cgttttatat tcaagaaaag ttactatcaa
    18541 tcatattgat gaaaaagaca aacacctttt gaaacaagat attgattata aatacgatgg
    18601 ggaaacgtat gaatataaac ctagaaccga tttatttgat aaagatggaa acaattataa
    18661 atctgatgta atccataaag ggaaaattga tggaaaagat atagttttaa acactcccta
    18721 tcatattccg gtactttctg tgaatgtgga tcggattcaa attgatacgg ctagagctgt
    18781 taaaaatggc tatttaccta caaaaataga actaagtaaa aaagaagaat atgaaaaaga
    18841 attagaaaaa attgtattca aagttaaaat tacagatata gataacaata aagttgttta
    18901 tgatgaaaac tttaaataca aagaatttaa tagtcatatg tcgcttgatt tacctacaga
    18961 atatttaaca aagaatgtca atggccattc aaaaatgtag gtttttggcc atttagaaat
    19021 gtcgtttttt ggccaccaaa aatgtaggtt tttggcccat aaataacaag gttacttata
    19081 cgacaacttt ttttctttat aatctttaat tcgataagac tttccagtaa tttgaataat
    19141 cttagcgtgg tgcactaatc gatcgattag tgcatttgtt aattttttat cttgaaaagt
    19201 ttcagcccat tgagacaaag gaatattcgt tgtaataatg gttgattttt tttcatagcg
    19261 tagattaatt aattgaaaga ataactttgc cccgtcaggg gtaaaaggca aataaccaat
    19321 ttcatcaata attagtaaat cgtaagaagc atattttttt aacaatcgat ctaaatttcc
    19381 acgttgattt gcacgattta gtcgattgac taattcattg cttaaagcaa aatggcaaga
    19441 ataacctttg gttatagctt ctagtgtaat ggcaatagct aggtgagttt tccccactcc
    19501 gctatttcca ataaacaata tattattatt tgtatccata aagcgtaagg tacacaaatc
    19561 taaaatctct gttcgattaa tctttggttg aaatccaaaa tcaaagtcat tgattgtttt
    19621 tttaaacggt aaattcgcct ttcttaaacg attttctttt tgtcgttcat ttcgtgagtt
    19681 tatttcttcc gtcattaaat gatttaaagc ttctacaaaa ctaagatttt cttgaacaat
    19741 ggtatcgaga tattctggta aaatttctct cgtatgtttt aaattcaaat cttctaattt
    19801 gtttagtaaa tgataataat tagacataat gattcacctc tctataattc gtcatacgct
    19861 tgtaaatttt catcaacaaa acgttctaat tcttcgtctt ctaaatgttt aaaaacgtca
    19921 gaacgtagaa tttctatata gtcatttcga tgataattaa gcaattgatg agtcatcgta
    19981 tgtgtgcgaa taagagtaga gtcatcgtaa atataaagtt tatcttcttt tacacgaagt
    20041 gtaagaattt taccgatgta atttactggc acagaatatt tattttgatt ccattgaacc
    20101 atcgcttcgt tagatacttt tcgtttaatt tgtttttcaa cagaagcata aattttaagt
    20161 tgattcgcat caaaagaaga tagttgttct ttctcgtatg accaacggtc actaggacat
    20221 tcatgaattg cttgagaaat ttcttcagta tttaatgaat ccatgattgt ttgaataatt
    20281 ttttctaatt cgtgagtaga ttcaaattca taattaaata cacgaattcg ttcaattgtt
    20341 cgtgcaagag cttcaacttt tccttttgtt tgaggtcgaa aagcacgaca agcaattgga
    20401 agaaaacctg cttctttcgc gtattgtcgg aaccgctcat taaaaataac tttagaaaat
    20461 tgactacgac tacggtcaac aacagttttc atattatcaa accatatttc tgtaggaaca
    20521 cctccggtgt attgaaaagc attagttaag caacgaaata aagtagtttg agaacgatca
    20581 aaagttagtt ctaaatactt aattctggaa tatcctaaaa cgtaaaggaa gatattacaa
    20641 gaaataactt caccgaattt attgactaat ttgaggtctt ctttccaatc aacttgagca
    20701 gataagccag gagttgtttc aacacgaatc gttgcttttt gtattttatc tgctttgata
    20761 ttgcgacaat atcttctcag tgttcgtgca cttccacgat agccacgttt agaaataaaa
    20821 tgataaatag cagaagaaga acaacctaaa gctaatttgt cagcgataat atcttcaaaa
    20881 tcactaacca aagttttttt gtttttattc ttttgagcaa gtaaaggtaa tttattgctt
    20941 attccagctt cataataacg ttttactgta cgataatcgc aattgtattg tttagctaga
    21001 gcagcataat taggttttgt atgagtcata atgaaaggta acactccttc ataaatatct
    21061 tttctcaata cactttcctc cttgaataaa ataacacaag gaaagcgtac cagatgacgc
    21121 ataaacctac atttttattt ggccattttt ctacatttta acttggccat ttacaacaaa
    21181 gaataaaaaa atcaattata gtgttgatat tcttttagtg gataatccag ataaaaacaa
    21241 gttcgaaact gaaactaaag atttaagaac gatcggttat acttcttctg aaaaagtttt
    21301 aacaaacaaa gatatgacaa ataaccaagt caactattca gcagtagtta gaacagtgaa
    21361 agaaagagag aataataatc cggtcaaaga gttcaatgag agtatcaatt ataaatttac
    21421 tgatagcact aaaacaaaaa caggatatgg tatatcagtt gatttagagc caacatatag
    21481 aaatgaaatt gggaaacaaa tagatatgaa agtctatagt tatccgatga atgaattgat
    21541 tgacagcaaa attgacgaaa tctatcaaag tgggggaaaa gaaacagaag ttaagatgga
    21601 tcgcttaaag aatgatggta aagttgagtc gggtattagt actcaaaatc tttcattcca
    21661 atttccacat atgaatatcg aaagaaaaac tggaaatata tatgctgaca gtcaaagaaa
    21721 tgatgaatct attaagtatg aattgatcga tggcggtaga aaattatatg tgcctatttg
    21781 gtgtgatttg ggaaattatt ctattgctat caaaagtagt aactttggtg gtaactatat
    21841 ctcgttggat atgaaccaga acatagatgt ttatgcttat atgtatgcaa cgatagatag
    21901 tgaaacaaaa aaagatgatg aattactgct tgaacctgtt tatccagagt ctactctacc
    21961 agatggttgg agcaaaaatg agattgattg gctgaataaa taagtttgaa aagtttttat
    22021 ctatctgata gaattaaata atgatatagg gatggtgata gaatgagggt tcaaagctat
    22081 ggtaacagtg tctttgatgt taaaagagaa tttcaagatt ctagaagaaa aaccaaaaag
    22141 ctgatagctt acttcattct atcagctatc gttttctata ttgtcaagtt tgttttggaa
    22201 ttaaactcaa tagtattcac agatatggtt cataaaatgt taaatggaga acctattaat
    22261 ttcaactttg attacatgct tgaaaaagca caaagcaatc aaattgtggc ttttatcgtg
    22321 agtatctatt attttattga aaaatctatt ttttctttaa aagatttttt ctttgaaatg
    22381 cttaccaaac taacaacgtt ttacaataaa atttcaaaaa attaaggagg aagtttgaat
    22441 gttagatcaa aatacagaca gaagttggtg gatgattggt gccgtgatcg taggtgccat
    22501 cttaatcggg acagctaaag tagcttttcc cgaagtgttt aatatcgtca ttacattttt
    22561 caaaaacatg atccctacaa acttaggata gttccactaa aaaattcacg tagtttgatg
    22621 cgtagaaaaa taataaagaa tgaaaaaggc tacataaagt gatttatgta gccttttttt
    22681 tggatggaag tgatgtaatg ctaagagata tgcccaaaat gattctttta atgtttgttg
    22741 ggttcttttt aattttagtg attgcgtact attcattaaa tggctataca caagattcgt
    22801 ctgtgaaagg tatgcaagaa acgttaagaa ctacagcaat agcaaacagg gatgatagta
    22861 ttcgagtaga aaaaggacga tttgctttaa ttaagtctga ttttgaaaaa gattttaaag
    22921 aaaaatttgg gaaaagtaaa aatctaaata atgaaaccgc tgattataaa tttgattatc
    22981 ttacagatgg gaaggacggt attaaagcaa ttaaagtgaa ggttgaaagt ggatcaaaag
    23041 aatatcaagc aacttgtatt ttaaatgttg caaatgatta gaggtgtgaa atttgcgtgt
    23101 tgtagaggaa aagaagattt ctccaaaaat cgaggaaact cctaaaagta aaattaatat
    23161 cgcaagtatt attaaaaaaa atggaattaa agaaaactct gaagcgatag aaggaaacct
    23221 tgatttttta actaagcttt tagaagaaga tagtggaaat tatgaaccac caattgaaga
    23281 tgcaaaatta aatatgattc gtgatgattt aaccagaaac tattcccaag aagttttaca
    23341 agctttaact gatgatgaaa ttaggcgaaa acttgtacaa gttgttttga aagaacatgg
    23401 tactttcttt aaaaaagata aagaggttgc agaatatgta gtacgtgaat gtattggaac
    23461 agggattatt gagcgaatta tgaccaacga tgaaattaca gatgttggtt ggaatggctc
    23521 tcaacttagc attgaaacta actctaacaa atggttgttt gacggtgata aattaaatat
    23581 cacgcaagag tatattgaac gtgttatttc taaatatgct gttgtaaatg atcgggcttt
    23641 taatagtggt aatcctatca tggatggaat gtttaaaaat gttcgtatat cagctacaca
    23701 catagataat tcaccagatg gctctacgtt ttctatgcgt gtggcaaggc ctagattagc
    23761 attgaataaa gaaaactttg aagattttgc tcctgattat attttggagc tatttgagaa
    23821 aatgatgaaa acaaaagcaa atatctgtat atcaggggag acaggtacag gtaaaacgga
    23881 acttcaaaaa ttactgctaa gttttattga taatcatgat cgtatgatta tgattgagga
    23941 cgttcaagaa actcacgcta aagaattatt tccggataaa gatattttct cttggatcac
    24001 tactcccaaa aaagcaattt cagatttagt aaaagcttct cttagaaata atccacgatg
    24061 gatcattgtt tcagaaacac gtggttctga agcttatgaa atgcttcaag cggttttgtc
    24121 aggtcactat gttgtaacga cacttcatgc ggttaatgca cgtgcgatac ctagacgttt
    24181 tgtaaatatg tgtgcaagtg gttttaaaat aaacgaaaat gcggttgagg aagacataaa
    24241 tcgttatttc gattttggtg tccatatcaa aaaaacagtg gttgatggta aagtgatccg
    24301 ttatttgaat gaaattgttg aattttctgc tagtgaaccg actacagttt ttaagcaaaa
    24361 atttatcaat ggaaagtttg tcattgaaaa tggtagtctt tccgaagatt ttaaagaaaa
    24421 aatggctgaa aaatttatta cgtttgaatt tcctgaagat caaactaata cagaagaatc
    24481 acttcctgaa gaagaagagg tgatttaagt tgaaagtaca ggctatgaca aagcgaaaag
    24541 gtctcatgga tgtttcggaa ctaaacgcct atcaagtagc gtatggtaaa ccgttagatt
    24601 atagagatta tttgaactat gttggattac cttgtttgtt tatgggtggc ttttcttttg
    24661 ctttatgtta ctactggtgg attgctttga tttgtggagt aattggaggc gtatatggct
    24721 ttaaagttat aatgcctaaa agtattaaac gctcttatga attaaagagt ttacaggaaa
    24781 gaaataaatt tattaacaac atgactcaaa ttttgaccga tgatagtaaa acagttacta
    24841 gagcgttatc tatagcaaaa cagcgaacaa aaggtgaatt aagagaagat attcaaatct
    24901 tagaatcacg tttacaaggt gctgataaat ttcagattgc tgaagctttt cgtgaaatca
    24961 acaataaata ttatagtgat gttgttttta cacaatactt tgagcaattg gaaacagcta
    25021 tttacgaagg tagaaataat tcagatacta tgaagcaaat aaagagctat cataatgatg
    25081 tgaaacgtaa aacagaaggt tttatgaaaa tcaaagacgg ctatcttcaa gatatgaagc
    25141 aaatgatgtt tattgtagct gtttttattg gagctataac ttttgctttt ggtttttcta
    25201 ctttctatga aggttttgct cgatcaccta tcggatgggt atttggatct atttactttt
    25261 tgattatggt tagcttcatg aagtcatttt tcaaacatta ttttgatgat gaaataatga
    25321 gtttgggggt aagtaattaa tgaaagttaa ggctttaaaa aagaaaagaa aatcaagtat
    25381 tagccccttg aaaattacga aaacagaaca taaaagcttt tggattaaat tactaggtga
    25441 tgtttattat caaaaattac gtgaaatgaa caattccaat attgcgatta ttaagtggca
    25501 aaaacaacgg ttaaaaaagt cattgatttt tatcctagtc ggattcttaa tgtatctatt
    25561 tttagggaaa gaatggtatt acttagccgg tggaattgct acttcagtag gtttttatgt
    25621 ttggcaagca aaatcaatca atggtatcta taatcaattt cggtttgaac gacacttgca
    25681 atttagtaaa tttacacggc tattgatccc ctatctgaaa caaaaaaaag atggcggaaa
    25741 tttatatggt gtttttgcaa aaatcgtaaa aagactagat tatgataccg ataaaaagct
    25801 attaatgaag cttatgcaag aaatgacaga tcagcctaac gatattagac cttttacgga
    25861 atatgcagaa cagacttctg gaacagacat gtccgtatta tttatgtcta cagtttatga
    25921 tattcgacaa ggttcggcag atttggatgt tattgaagag ttggatcgtt tagcttctga
    25981 agagttaatg gccggaatcg attcaatcat tgactataag tcgaggcgtt ttgtcttctt
    26041 tcctacaaaa attgcaatga cttcatttat tttagttgtc ggttttgcag taggcgttct
    26101 tttcatgaac ctaaaagata ttaactttgg gcaataaagg aaaggagaaa gtcaatgcaa
    26161 gaacaatccg ttatggaaat cattaaatgg tttataggtt taatgatgat tatggcaatg
    26221 gtttctatag gacttttttg tgttcaatta caagatatta atactttcaa gcaacaagta
    26281 aattatcaaa ttgaacgaaa aggaggtctg acgacagaag ctgttgaagc aatcgacaaa
    26341 tattcggcta ctaattatga tagacgtttt acaattgaaa gcgataagtt aaatcagaaa
    26401 gtatcttatg gagaagaaat tgattatacg atcaaagcaa ctattccaat tcaaatttta
    26461 ccgcttcctg atgtaacttt atcgtttaac ggaaatagtg tttctcaaat aagatgagag
    26521 gaaggataaa atgcaagaga ataatgtttc cagaataatt tatttgattg gcggattgat
    26581 aattttatcc ttagctattg ccggtcttgt agagcattgg ccggaaataa agttattaat
    26641 aagaaacttt tacgacaata aaatgattaa aatgtaggag gtatagattt ggaagcaatt
    26701 caagaaaatc gtaaggataa tcaagaaaca agtgaaaaaa acaaaaaaaa aagaaaaaag
    26761 aaagtagata aatttacgga aatgaccgat aaaatcattg aaaagaaagg acttaaacct
    26821 aaagactttg accgtcaagc aatcaaagaa gcacgacaaa aactgtatga tgattttgtt
    26881 aaagaaaaca tatcacagct tttagactaa catttagtct aatggtgata agtcaagatg
    26941 aaatttaaga t
//
LOCUS       DACEKQ010000260        23731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.042, whole genome shotgun sequence.
ACCESSION   DACEKQ010000260 DACEKQ010000000
VERSION     DACEKQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.042"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..567
                     /locus_tag="IW671_002991"
     CDS             292..567
                     /locus_tag="IW671_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899906.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2658
                     /locus_tag="IW671_002992"
     CDS             580..2658
                     /locus_tag="IW671_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0899907.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2818..4704
                     /locus_tag="IW671_002993"
     CDS             2818..4704
                     /locus_tag="IW671_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0899908.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4716..5663
                     /locus_tag="IW671_002994"
     CDS             4716..5663
                     /locus_tag="IW671_002994"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0899909.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5683..6210
                     /locus_tag="IW671_002995"
     CDS             5683..6210
                     /locus_tag="IW671_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0899910.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6273..6926)
                     /locus_tag="IW671_002996"
     CDS             complement(6273..6926)
                     /locus_tag="IW671_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0899911.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7058..7900
                     /locus_tag="IW671_002997"
     CDS             7058..7900
                     /locus_tag="IW671_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0899912.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8058..8954
                     /locus_tag="IW671_002998"
     CDS             8058..8954
                     /locus_tag="IW671_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0899913.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8957..9670
                     /locus_tag="IW671_002999"
     CDS             8957..9670
                     /locus_tag="IW671_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0899914.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9690..10208
                     /gene="msrA"
                     /locus_tag="IW671_003000"
     CDS             9690..10208
                     /gene="msrA"
                     /locus_tag="IW671_003000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0899915.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10205..10432
                     /locus_tag="IW671_003001"
     CDS             10205..10432
                     /locus_tag="IW671_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0899916.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10584..11135
                     /gene="lepB"
                     /locus_tag="IW671_003002"
     CDS             10584..11135
                     /gene="lepB"
                     /locus_tag="IW671_003002"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0899917.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11218..11760)
                     /locus_tag="IW671_003003"
     CDS             complement(11218..11760)
                     /locus_tag="IW671_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0899918.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11997..12395)
                     /locus_tag="IW671_003004"
     CDS             complement(11997..12395)
                     /locus_tag="IW671_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0899919.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12655..13515
                     /gene="ylqF"
                     /locus_tag="IW671_003005"
     CDS             12655..13515
                     /gene="ylqF"
                     /locus_tag="IW671_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0899920.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13508..14275
                     /locus_tag="IW671_003006"
     CDS             13508..14275
                     /locus_tag="IW671_003006"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0899921.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14331..15188
                     /gene="dprA"
                     /locus_tag="IW671_003007"
     CDS             14331..15188
                     /gene="dprA"
                     /locus_tag="IW671_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0899922.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15310..17388
                     /gene="topA"
                     /locus_tag="IW671_003008"
     CDS             15310..17388
                     /gene="topA"
                     /locus_tag="IW671_003008"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0899923.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17357..18733
                     /gene="trmFO"
                     /locus_tag="IW671_003009"
     CDS             17357..18733
                     /gene="trmFO"
                     /locus_tag="IW671_003009"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0899924.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18931..19836
                     /gene="xerC"
                     /locus_tag="IW671_003010"
     CDS             18931..19836
                     /gene="xerC"
                     /locus_tag="IW671_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0899925.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19873..20421
                     /gene="hslV"
                     /locus_tag="IW671_003011"
     CDS             19873..20421
                     /gene="hslV"
                     /locus_tag="IW671_003011"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0899926.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20435..21835
                     /gene="hslU"
                     /locus_tag="IW671_003012"
     CDS             20435..21835
                     /gene="hslU"
                     /locus_tag="IW671_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0899927.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21853..22644
                     /gene="codY"
                     /locus_tag="IW671_003013"
     CDS             21853..22644
                     /gene="codY"
                     /locus_tag="IW671_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0899928.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22726..23601
                     /locus_tag="IW671_003014"
     CDS             22726..23601
                     /locus_tag="IW671_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0899929.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8212 a         4092 c         4999 g         6428 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
     2701 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
     2761 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
     2821 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
     2881 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
     2941 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
     3001 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
     3061 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
     3121 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     3181 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     3241 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     3301 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     3361 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     3421 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     3481 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     3541 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     3601 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     3661 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     3721 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     3781 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     3841 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     3901 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     3961 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     4021 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     4081 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     4141 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     4201 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     4261 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     4321 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     4381 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     4441 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     4501 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     4561 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     4621 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     4681 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     4741 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     4801 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     4861 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     4921 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     4981 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     5041 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     5101 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     5161 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     5221 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     5281 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     5341 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     5401 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     5461 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     5521 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     5581 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     5641 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     5701 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     5761 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     5821 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     5881 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     5941 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     6001 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     6061 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     6121 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     6181 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     6241 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     6301 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     6361 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     6421 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     6481 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     6541 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     6601 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     6661 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     6721 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     6781 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     6841 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     6901 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     6961 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     7021 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     7081 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     7141 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     7201 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     7261 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     7321 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     7381 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     7441 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     7501 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     7561 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     7621 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     7681 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     7741 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     7801 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     7861 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     7921 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
     7981 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
     8041 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
     8101 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
     8161 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
     8221 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
     8281 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
     8341 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
     8401 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
     8461 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
     8521 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
     8581 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
     8641 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
     8701 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
     8761 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
     8821 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
     8881 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
     8941 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
     9001 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
     9061 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
     9121 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
     9181 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
     9241 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
     9301 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
     9361 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
     9421 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
     9481 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
     9541 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
     9601 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
     9661 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
     9721 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
     9781 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
     9841 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
     9901 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
     9961 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
    10021 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
    10081 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
    10141 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
    10201 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
    10261 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
    10321 tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga
    10381 tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt
    10441 tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt
    10501 atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa
    10561 aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc
    10621 ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat
    10681 tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg
    10741 ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag
    10801 atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa
    10861 atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga
    10921 aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac
    10981 aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag
    11041 atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct
    11101 attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt gcagtctgtt
    11161 gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta cgtaaaatca
    11221 aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat aaacaaccat
    11281 tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa ttactttgaa
    11341 atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga ctgggatcat
    11401 aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatgat ataaaaataa
    11461 gccattaata aatccaccaa ggaaagccgt gataagaaaa gcagcttgaa acgttcctga
    11521 accaaatatc gtgaaattta aagtgttcga caacatttca agcaaaccag cccagatcgg
    11581 tccatataga taacccataa gcgccatact taaaaatccg aatgtgattt gaaaataagc
    11641 aggaccaatc gctatcttgt ataagatgac atcaagggct aataaaagag cagaggtcga
    11701 caacaatctt gcattcattt cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa
    11761 aatattctct cctttgtcag ttaataataa aaggatacca taaaatcacc aatgtgtcat
    11821 gacacattgg tgatttacat acactggcat ctaacaatta aaaaaagggt gagaagactg
    11881 cttttataaa ctgtaaaaaa gagattgtac gaataaaaaa aatagaggcg gagaatactt
    11941 atcgtatcat ccgcctttgt ttttttgtta taaaaaacgt cagattagtt atctgcttac
    12001 atcttcatcc atacgggttc taatgtattt ccgtctagat cttggacttc taatgaaaac
    12061 atttcttctt caggcattcc catatctact tcgtggaaag taccaccatt ttcttttgca
    12121 atttgagcaa atttttttac ttcatccgca ttttcaacac taaacgaaat caaagcacca
    12181 cttgtttttt tattatcagc tatttcttta tcttttataa attttttata aaaatcatga
    12241 gagagaagca ttatccaaaa agtgtcatcc cataccattg aaccagcttc ttcagtagaa
    12301 aattcttcat tttttttaaa acccagcttt tcataaaaag caacggaacg attgacatca
    12361 gcaacaggaa aattaacaaa aaccatacta ttcatttgtt tcgtctcctt tctgttttgt
    12421 tcgtctatca tctcttattt aggaaacgct gtcaaaaatt tagattcata tatagaaacc
    12481 aaaataaaaa tttgcatcat caaaaaagtg aagatgtgat agaatgagga ctggttcact
    12541 ttcacttaac taatagatgg aatttcttta taataaaaaa gtgaatttat attgaaaaag
    12601 aaaaaaacaa gtaaagccgg ggaatattcc ggtatgaaag gaatggagag aacaatgacc
    12661 atccaatggt ttccgggaca tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa
    12721 tatgtggaca tcgtatttga attggtagac gcacgattac ctttatcttc tcgtaatcca
    12781 atgctagatc agatcattca gcaaaaacct cgccttgttt tattgaataa agcggatctg
    12841 gcagatccac aacagacaaa gctgtggcaa gaatattttc gtaaacaagg ccatcttgca
    12901 ttagctatca cggcacaaga aagtaaagga attaaaggtc tagtacctgc ggctaaagaa
    12961 gcattgaaag aaaaacgggc aagagatgct gctcggggga tcaagcctcg cgctattcgg
    13021 gcaatggtct taggtattcc aaatgttggg aaatctacgt tgatgaatcg actagtaggc
    13081 aagaaaattg cacagacagg aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt
    13141 ttaggtaatg agttggaatt gttagacaca ccagggattt tatggcccaa atttgaagat
    13201 gaagaaatcg gcaaaaaact agcgctgaca ggcgcgatca aagaccagct tgtccattta
    13261 gatgatttag cgatttacgg attagacttt tttgcccgat attattcagg aagaatcagc
    13321 gatcgttatg gtttatcaat cgagcaagaa gaaaaattgg caccggaatt actgatggat
    13381 attacagcaa aacgtgggta taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat
    13441 gatattcgtc aaggaaaact tggacgatac acactggatc gttgcgaaga aatggagcaa
    13501 aaaagtgatg actgaatcca tccaaagtat caaagaacga ttgaaaagca tacagagaat
    13561 agatgatcct acactagaat cattaagaaa tgatccacgc aaaggcgtgc agcaagcact
    13621 ggcttctttt gaacgacgat tgaaaaagga agcattaatt aaggaaaaat atgaagaaat
    13681 gttttgcttt gaaaagcaag ggtacgcaca aggtcaccaa tggattgccg gcattgatga
    13741 agtaggaaga ggaccattag ctggacctgt cgtggcagct gcagttatct tgccgaaaaa
    13801 tgagatcata ttagggctga atgactccaa acaattatca gagaaaaagc gagaacttct
    13861 ttatcaacaa gttcaagaaa aagccctggc aatcgggatc gggatcgttg atcagcagac
    13921 aatcgatgat gtgaatattt atgaagcaag taaattagcg atgatccaag cagtagaaaa
    13981 attgtccatt gtaccaagct atttgttgat cgatgcgatg accttggatt tgcctattca
    14041 gcaggaaaaa ataatcaaag gggatgcacg atctgtgtcg attgctgctg caagtatcat
    14101 tgctaaagta tatcgagacc aattgatgaa agaatatgac gtgctttatc ctggatatgg
    14161 tttctcgcat aatgcaggtt atggaacaaa ggaacactta gaaggacttg aacgacaagg
    14221 aattacgcca atccatcgta aaagttttgc acctgtaaaa aaatatttac aataaaaaac
    14281 agccgctttt acgtatctta ttagaaagag agacgaaagg cggttttatg atgtatcaaa
    14341 tagaagaaaa tttattgaaa cttgcttata caaaaggaat cagtagtcaa ggaaaatggg
    14401 cacttgctaa ctggatgatg cagtatcctt ataaagaaat cggatttgaa gacatcatta
    14461 agataggtaa aataaccaaa caccgggagc tgtttcgcaa aagttggcag gaaattcacg
    14521 ctaactgggg caaaatccaa tcgaaacagt cgtttatcac ttgctttgat cccaaatatc
    14581 cccctcagct gcttcacttg acctatccgc caattgtact gttttataat ggtgatctat
    14641 ctttactttc ttgtaacatg ttaagcgttg ttggtggaag acacacttct gctttagcca
    14701 aaaaaacagt ttattattta ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg
    14761 gctgcgcgaa agggatagat acatacggtc accaagcagc aatctctcat ggtggacgga
    14821 caatcgcagt cattggaaca gggatagagc aagtctatcc tgctgaaaat cgtgacttgc
    14881 aagaggtgat tgcaaatgat catcttcttt tgagtgaata tcctcctcaa tccggcccga
    14941 aaaagcattg ttttccaatg agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg
    15001 tctttgaagc caagaaaaaa agcggctctc tgataacagc acagcaagca ttggatcttg
    15061 gaagaacagt tttttctgtt ccaggaaata ttttaacaga acattctacc ggatgtcatc
    15121 atttgatcca agacggtgca gtttgcacgg tttcgggtga agatatcttg gcagaattta
    15181 aagaataaca ccatactttt catttgcttt tcttgacaaa cgttttctga atggatatga
    15241 ttagctacga tttgtatgaa tcctctttta atagtaggaa cacgacaatg gaaaggagtc
    15301 cattcagtaa tggcgtataa atatttagtg attgtagaat cacctgcaaa agccaagaca
    15361 attgagaaat atcttggacg aaactataaa gtaatggcca gtgttggcca tataagagat
    15421 ttaccgaaaa gtaaaatggg tatcgatttt gaaaacaatt atgaacccca ttatatttct
    15481 atacgcggaa aaggcgatgt catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa
    15541 gtttacttgg caagtgaccc ggatagagaa ggagaagcga ttgcttggca tttagcgtac
    15601 ctacttgggt tggatctgaa agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac
    15661 gcagtcaaag cagcttttaa ggaaccaaga acgatcgatg tagatttagt agatgcacag
    15721 caagctcgtc gtaccttaga cagaatcgtt ggttattcga tcagtcctat tctctggcgt
    15781 aaggtcaaga aagggttaag tgcaggacgt gtccaatctg tcgctttaaa aattattatt
    15841 gaccgtgaaa aagagatccg agaatttgtt ccagaagaat attggagcat cgacggtaat
    15901 tttaaaaaag ctcgcaagaa attcaaagca aatttctggg gaatcgacgg taagaaaaag
    15961 aaattaccag atgcacaaag tgtaaaagaa gtcactgcta gaattgatgg aacagacttc
    16021 gaagttaaaa aagtagaaaa gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca
    16081 agtagcctgc aacaagaagc tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg
    16141 gtagcacagc aattatatga aggaatcact ttgggtaaac aaggaacagt cggcttgatt
    16201 acatacatgc gtactgattc tacccggatt gccgatacag caaaagcaga ggccgcagca
    16261 tttattgaag atacgtatgg aaaagaattt tctagacatg atcaccgaaa gatcaaaaat
    16321 gctcaaggcg cacaagatgc ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca
    16381 gatgaactca aacaatattt agataaagat caattgaaac tttatacatt gatctggtca
    16441 cgttttgtgg ctagtcagat gacaccagct attttagata caatgaaagt gacattagaa
    16501 caaaatggtg tcatcttcat tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa
    16561 gtgtatgtgg aaggtagaga tgacggcaaa gaagagaaag aaaatattct tccagaactt
    16621 gaagaaggcg atgtagtcca atctgttgat attgaaccaa aacaacattt tactcaacca
    16681 ccagcacgtt ttagtgaggc tacgttgatt cgtgcactag aagaaaacgg ggtagggcga
    16741 ccttccactt atgcaccaac acttgatacg atccaaagac gctactatgt aaaattgaca
    16801 caaaaaagat ttgagccgac tgaactagga gaaattgtca attcattaat ctgtgaattc
    16861 ttccctcaaa tcgtcgatat ccatttcaca gcagaaatgg aaggagattt ggataaaatt
    16921 gaagaaggca cggaagcgtg ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa
    16981 gaactgacga atgcggaaga aaaaatcgaa aaaattcaaa tcaaagatga accagcagga
    17041 ttcgattgtg atgtctgtgg acatccgatg gtgataaaat tagggaaata tgggaagttt
    17101 tatgcttgca gtaattttcc tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt
    17161 gtgacttgcc cagtatgtca tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga
    17221 ctattctatg gttgttcccg tttcccagaa tgtgaattca cttcttggga taaaccagtg
    17281 ggcagaaatt gtccaaaatg tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa
    17341 caagtagtct gtgtaaatgg agattacgaa gaagacatcc aaaaataata gaaaagaggt
    17401 tctttcattg aaaatggtaa atgttgttgg agcagggctt gccggaagtg aagctgcttg
    17461 gcagatcgca caagcaggag tccctgtccg tttatatgaa atgcgtccag tcaaatcgac
    17521 tgaggcgcat cacacaagta attttgctga actagtatgt tccaattcat taagaggaaa
    17581 cagtttggct aatgcagtag ggttattgaa agaagaaatg cgccgattga attcggtagt
    17641 aatcacttcg gcagatgaaa cagcagtgcc agctggtggt gccttagctg ttgaccgcga
    17701 ttctttttct gaaacaatta cccgaaaagt caaagagcat ccattagtaa cagtgataaa
    17761 cgaagaaatt actgaaatac cggaaggaat caccgtagta gcaacaggac ctttgacttc
    17821 tcatccatta gcagaaagca tcaaagcatt caatggttca gatggttttt atttctatga
    17881 tgcagctgca ccaatcgtag acaaagctac gatcgatatg gataaagtat atttgaagtc
    17941 ccgctatgac aaaggagaag cagcttactt gaactgtccg atgacagaag aggaattcaa
    18001 agctttccat gaggcactaa tcaatgccga agtggcagaa cttaaatctt ttgagaaaga
    18061 aaaatatttt gaaggatgta tgccaattga agtaatggca cagcgaggat tcaaaacgat
    18121 gcttttcgga cctatgaaac cagttggatt ggaagatcct aaaactggca aacgtcctta
    18181 tgctgttatc cagctgcgtc aagacaatgc tgcagcttct ttatacaaca tcgtgggctt
    18241 tcagactcac ttgaaatggg gcgagcaaaa acgtgttttc cggatgattc ctgggttaga
    18301 aaatgctgaa ttcgttcgct atggtgtgat gcaccgaaac agctttatga attcgccaga
    18361 attgctgaaa ccgacttacc aatcaacgaa acgcgaagat ctgttctttg caggtcagat
    18421 gactggtgta gaaggctatg ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc
    18481 cgcacgctta gcaaaagaaa tggaaccagt cgagtttcca caagaaacag ctattggaag
    18541 tatggcttac tacattactc atgcggaagg aaaacatttc caaccgatga atgccaacta
    18601 tggtctgttt cctgaactgc cagagcgcat tcgagacaaa aaagcaagat atgaagcaat
    18661 tgcacaacga gctttaaaag ctaatgaggc aataaaaaca gaattagaaa aaactgcaat
    18721 cccaaatgag taaggaaact ccatgaagag ggtgagacgg atagtctcgc tctttttctt
    18781 gcgtttttct taagaatttc tcaaacaaaa tgtgaggaat tgcgccaatt gtgaacaaac
    18841 ttagaaatat tggttaaatt ctgacaattt atttgtcttt ttataaacgc tgtgctaaat
    18901 tattctctgg gacaaaagga aggtgagaaa atgacagaag ccacgtggga acaaaaattt
    18961 ttgcgttatt tgattgtcga aagaggatat tcagaaaaaa cgaaagaagc atatgaagaa
    19021 gacattctcc atttcaaaca ttttttggag gaatctggag acgctgatct gctgaaggcg
    19081 gaatatttta cggttcgtgt ctatttgagt gcattagctg atcatcgtcc tagctatagc
    19141 agaaattcta tcagtcgcaa gatagccagc ctacgttcgt tttatcagta tcttctcaaa
    19201 gaagaagtga taaaagaaaa tccattttct tacgttcatc tcaaaaagaa aaatttgaga
    19261 ctgccacgtt ttttttatga aaatgaaatg caagcacttt ttgacagcgt agcaggagat
    19321 agtcctttgg atttgaggaa ccgcgcttta ctggaagtcc tttacgggtc aggcatccgt
    19381 ttaagtgagt gtagcagttt gtcggtatca gacattgatt ttgattcaga ggtcatgttg
    19441 atccatggta aagggaacaa agagagatat gctcctctag gttcttttgc tcaagatgct
    19501 ctgcaagaat atttcaggga aggccgccag gtattgatga caaaatatca cgaagagcat
    19561 gatatcgttt ttgtgaatca tcatggaaaa ccaatcacgc ctactggaat tgaatatgtc
    19621 ttgaatcaaa tgatcaaaaa aagcagtttg gatagtgata tccatcctca catgctgcgc
    19681 catacatttg ctactcattt attaaataat ggggcagatt tgcggacagt ccaagaattg
    19741 ctaggacatg ccaatttatc gactacccag atctatgccc atgtaacaaa agaaagttta
    19801 caaaaaaact atcgttcttt ccatccaaga gcgtaaaaat aaggaaaaga acaaatttag
    19861 gaggaaacaa acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa
    19921 gacgggaaat ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg
    19981 aaaggcactg caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc
    20041 ggaagtgttg cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat
    20101 aacgggaatc tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct
    20161 atgcaaaaac ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca
    20221 ggtacaggag aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat
    20281 tttgcgttgg ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa
    20341 gaaattgcag aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat
    20401 attatcgtag aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag
    20461 acaaatcgta agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt
    20521 tgccgttgct ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga
    20581 tattacacca aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc
    20641 acgacgtttg gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac
    20701 tgaagtcggt tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc
    20761 gatccaaatc gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc
    20821 agaaaaacgt ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac
    20881 taatcaattt gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc
    20941 gcaagaagaa gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca
    21001 aaagggttta ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc
    21061 accaatgatg aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc
    21121 tttgacaccg aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt
    21181 gatcaaggaa gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa
    21241 attagcggaa gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc
    21301 acagcaatct ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga
    21361 aggttcacaa gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc
    21421 ttcaggagct ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt
    21481 cccgatacgg gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga
    21541 accaaataat gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt
    21601 tacgtttaca aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga
    21661 cacagataat atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct
    21721 attatttgaa gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga
    21781 tgacaaactt ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat
    21841 cggaggacga caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag
    21901 caaaagaata cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta
    21961 ggtggtattc ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat
    22021 aatgaacgtc acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt
    22081 cctgaatctt atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt
    22141 actagtgata ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg
    22201 actgtcgttc cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg
    22261 gaacaatcgt ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt
    22321 atgcagatcc tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct
    22381 gtccaaatgg caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt
    22441 gaagcattgg atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc
    22501 atcactcgtt ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa
    22561 tctcgttcgt taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac
    22621 gagttagcaa aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc
    22681 cagccttccc attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt
    22741 caaaatgacc agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa
    22801 tcaaaagaac atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat
    22861 gcaccggttt tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg
    22921 aaaacatatc aaatggggca acacggattt gctcgggata tggattttga agtcattgaa
    22981 catggacaag aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat
    23041 ccatttgatt ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat
    23101 tatcaagtgg caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct
    23161 ttcaatgtac ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca
    23221 ttgaaatcaa gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg
    23281 ttaggtcaga caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg
    23341 gtttttgaaa caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc
    23401 gtaactttaa gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa
    23461 gcgcctttcg tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga
    23521 aacttgatgg aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa
    23581 tattccatca cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac
    23641 caaggtgtgt aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtggct
    23701 ctttgtcaat aaggactgat gagttgtgca a
//
LOCUS       DACEKQ010000261        18794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.060, whole genome shotgun sequence.
ACCESSION   DACEKQ010000261 DACEKQ010000000
VERSION     DACEKQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.060"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1109
                     /locus_tag="IW671_003015"
     CDS             213..1109
                     /locus_tag="IW671_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0899930.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1259..2200)
                     /locus_tag="IW671_003016"
     CDS             complement(1259..2200)
                     /locus_tag="IW671_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0899931.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2326..3447
                     /locus_tag="IW671_003017"
     CDS             2326..3447
                     /locus_tag="IW671_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0899932.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3705..5207
                     /locus_tag="IW671_003018"
     CDS             3705..5207
                     /locus_tag="IW671_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0899933.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5274..5801
                     /locus_tag="IW671_003019"
     CDS             5274..5801
                     /locus_tag="IW671_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0899934.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6012..7073
                     /locus_tag="IW671_003020"
     CDS             6012..7073
                     /locus_tag="IW671_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0899935.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7076..7972
                     /gene="murQ"
                     /locus_tag="IW671_003021"
     CDS             7076..7972
                     /gene="murQ"
                     /locus_tag="IW671_003021"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0899936.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7986..9968
                     /locus_tag="IW671_003022"
     CDS             7986..9968
                     /locus_tag="IW671_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0899937.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10033..10479)
                     /gene="nrdI"
                     /locus_tag="IW671_003023"
     CDS             complement(10033..10479)
                     /gene="nrdI"
                     /locus_tag="IW671_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0899938.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10690..12114
                     /locus_tag="IW671_003024"
     CDS             10690..12114
                     /locus_tag="IW671_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0899939.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12295..13068)
                     /locus_tag="IW671_003025"
     CDS             complement(12295..13068)
                     /locus_tag="IW671_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0899940.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13065..13970)
                     /locus_tag="IW671_003026"
     CDS             complement(13065..13970)
                     /locus_tag="IW671_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0899941.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14124..14444)
                     /locus_tag="IW671_003027"
     CDS             complement(14124..14444)
                     /locus_tag="IW671_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0899942.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14918..15604
                     /locus_tag="IW671_003028"
     CDS             14918..15604
                     /locus_tag="IW671_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0899943.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15606..16550
                     /locus_tag="IW671_003029"
     CDS             15606..16550
                     /locus_tag="IW671_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0899944.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16749..17393
                     /gene="pcp"
                     /locus_tag="IW671_003030"
     CDS             16749..17393
                     /gene="pcp"
                     /locus_tag="IW671_003030"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0899945.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17472..18749
                     /locus_tag="IW671_003031"
     CDS             17472..18749
                     /locus_tag="IW671_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0899946.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6036 a         3314 c         3993 g         5451 t
ORIGIN      
        1 atatttaaca atgactaatg ataacaaatt ttcagacaat tacaactatt cagaaaactt
       61 ttaggcgaat tttaatagaa gaatgattta tttcatcata ttttcataca aatgttaaat
      121 tttcatacaa atataggaaa cgttttactg gctttttact atttaaacag gctatcatag
      181 gaaaaagtga cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg
      241 caaaaaataa atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa
      301 aagatttact acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc
      361 tgatcgtttt tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta
      421 ccaatgcaaa aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc
      481 tgaagcaggc acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag
      541 caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt
      601 ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag
      661 cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc
      721 cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag
      781 gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga
      841 catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt
      901 atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac
      961 cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg
     1021 cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat
     1081 atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg
     1141 aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta
     1201 ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt
     1261 attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg
     1321 gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt
     1381 cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt
     1441 tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc
     1501 cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg
     1561 ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg
     1621 ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat
     1681 ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt
     1741 ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta
     1801 gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca
     1861 gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa
     1921 tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca
     1981 agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga
     2041 ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag
     2101 caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc
     2161 ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc
     2221 aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa
     2281 aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg
     2341 gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat
     2401 ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat
     2461 actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat
     2521 ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc
     2581 gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa
     2641 actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa
     2701 gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga
     2761 ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct
     2821 actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc
     2881 gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga
     2941 caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct
     3001 cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca
     3061 ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta
     3121 gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga
     3181 gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt
     3241 gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa
     3301 ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa
     3361 gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc
     3421 agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat
     3481 ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag
     3541 ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc
     3601 ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc
     3661 agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa
     3721 taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc
     3781 taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa
     3841 ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag
     3901 aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa
     3961 atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag
     4021 atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc
     4081 tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc
     4141 atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct
     4201 tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa
     4261 tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa
     4321 aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc
     4381 taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa
     4441 agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg
     4501 ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga
     4561 tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata
     4621 gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa
     4681 atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag
     4741 gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca
     4801 aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt
     4861 atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag
     4921 catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg
     4981 agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag
     5041 ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat
     5101 tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct
     5161 tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc
     5221 aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt
     5281 atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag
     5341 atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag
     5401 atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag
     5461 cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa
     5521 tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt
     5581 tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg
     5641 ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata
     5701 atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta
     5761 cattcatggt agataataca gaattggttt cttatgagta attatgtttt tgtgttattt
     5821 aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat
     5881 tcttttgata atataaactc aacctttaga aatgaaattt aattatttta ataaaagtat
     5941 tgaaattttg tttctttaga tatatactct agtcatagaa atgaagacgg atacagaaaa
     6001 agagggataa tatgtttggc ttttcaatat ttatgaacga ttctttgtca gaagagaaga
     6061 aacagtatat ccgccaaatg gcatccaatg gctttgtcgg tattttcact tccatgcata
     6121 taccagaaga tgatgtttcg gcatatcaaa agcgattgat cgaactagga agcgaagcga
     6181 aaaagaataa actagaattg atggtcgata tttcaggaga ggcattggaa cgagcgggtt
     6241 tttcattaga agatctaacg cctttgaaag caattggtgt aacaggttta agaatggatt
     6301 atcatatttc caatcagcag attgccgact ggtcacatca attgaaaatc agtttgaatg
     6361 ctagcacgat cacgcctaaa gatatcgatg aattgaaaga agcagaagct gatttctcac
     6421 agattgaagc ttggcataat tattatccac gtcccgaaac agggctggac aaagaatggt
     6481 atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt tttgtaccag
     6541 gagatacaga gttgagaggc ccactatatc aaggattgcc aactttggaa gaacatcgag
     6601 ggatccatcc tcttgcaggc gctctagatc tattagcaag taacacggat attgtttaca
     6661 tcggagatgc aggtctgtca gaaaacgttc aggagcaatt tgcttctttc caaaaagaac
     6721 agacagtctt gctccatacg gaacctgtag atgaagaatt ttacgaatat atattaggga
     6781 agcatacgaa tcggcaagac gatgcaagag atgtcatacg aagcgtagat gcacgttttc
     6841 gagagattcc gcctatccca gcaagaaata cagctacgcg tatgaaaggg tctattactt
     6901 tagataacga aaagtatttg cgctatatgg gtgagatcca attaaccaaa tatgatttgc
     6961 cagcggatga aaaagtgaat gtagtagcta aagttatcaa agaagagcta ccattgatca
     7021 accaaatcaa agctggtatg aactatcaat ttataagaaa agaaggaaga taaaaatgag
     7081 caaaatcaat ttagagaacc taacaacgga acgcagaaac caagaaactt ttggattaga
     7141 tgagatgagt atttctcaag ccgttcaact aatgaacaaa gaagatcaga aagtggccgc
     7201 tgcggttgaa aaagaactga caagtatcga accagtgatt gctaacacga ttgagtcctt
     7261 taaaaaaggt ggacgtctga tttatatggg agccggaaca agtggaagac taggtgtgtt
     7321 agatgctgct gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca taggtctaat
     7381 tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt cttatgagct
     7441 agggcaagaa gatctaatca agctgaattt gacaaaaaat gatatagtta ttggtatcgc
     7501 agctagcggt cgtacaccat atgtgatcgg tggattagat tacgcaaaat cgatcggtgc
     7561 tgtcacagga acgatttctt gcaataaaca agccgaaatc agtaaacacg cggatttccc
     7621 aatcgaagtc gatgcaggtc cagaattttt gacaggctct actcgattga agtcaggaac
     7681 agcacaaaaa cttatcttga atatgatttc tactatcagt atgattggta taggtaaagt
     7741 attcaataac ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg aacgctcaaa
     7801 acgaatcatc atgcaagcga cagaagctga ttacgaaaca gctgaaaagt actttgaaga
     7861 agcagatcaa aatgttaagc tggctatcgt catgattttg acaaatagtg ataaagagga
     7921 agctcaagca aaattagctg aagcaaacgg atttgtaaaa gggaccttat gagggggaaa
     7981 agaccatggc aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt tataaagggg
     8041 caggcggcat gggcaatgta gaaagcattg tccactgcat gactcgtgtg cgtatgaaga
     8101 tcatcaatga tgatcttgta aatgtctctg aattaaaagc gattccagga gtgcttggtg
     8161 tagtagaaga tgaacagctt caagtcatca tcggacctgg gaaagtgaac aaagtagcac
     8221 agcaaatggt tgatttggca ggcgtgaaat taggagaaaa actttcttct gtaagtacag
     8281 ctacagcaaa ttcatcagca tctggaaaag accgtgtaaa cgaacgggca caagaaatga
     8341 aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta aaagacattt
     8401 ctaatatctt tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg gcagggattg
     8461 ccgcagtttt agcaaactta gtcacagccg gaacacttga tgcagcaaca tggcaacaat
     8521 atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt atctatacag
     8581 gtatcaatgc tgcacaagtt tttggtgcaa caccaactct tggtggagtt atcggggccg
     8641 ttgtgatgct gacagggatg aatccggaag caccactgaa aaacttattt actggtgaag
     8701 ctcttgcagc tggacaaggt gggatcctag gtgtcatctt cgctgtttgg ctattgtcaa
     8761 tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt agatatcatc gttacaccaa
     8821 ctctttcgtt gattgccatc ggtctgattg aaattttctt catcatgcct ttagcaggtt
     8881 tcatttctga tggcatggtc ggtgggatca actgggtact tggcgttggt ggagcatttg
     8941 ccggcttcgt tctaggcaca ttattcttgc cgatggttat gttcggactg catcaaattt
     9001 tgactccaat ccatgtgcaa atgattgatg aaactggtcg tacgctattg cttccaattc
     9061 ttgcgatggc tggtgcagga caagtgggtg cagcattggc attatggatt cgctgcaaaa
     9121 aagataaaga attaacagaa atgatcaaag gagctttacc agtcgggatc ctaggaatcg
     9181 gcgaaccatt gatttacgga gtaacattac ctctaggacg cccatttatt actgcttgta
     9241 tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg gctatcgcta
     9301 ttggaccttc aggagccgct ttgatcccat tgatctctga cggtaaatgg ttaggctaca
     9361 tccttggatt attggcagct tatgctggtg gcttcgttgc tacattcttc ttcggtattc
     9421 ctaaagaccg tttagaaaaa gaagaattag cgactgaaac agccacagta aatgaagtgc
     9481 ctgaaaaagt agtacctcaa acttcagctg aaagtgtgac tatcctctca tcagttgctg
     9541 acggaacagt tgtttcttta gataaagcaa gtgatccagt ttttgcacaa aagatgatgg
     9601 gtgaaggcta ctttgtagaa ccagaaaacg gacatatcta ttctccagtt actggggtag
     9661 tcagctctgt cttcccaacg aaacacgcta ttgggatcac aacgccaaat ggtttagaaa
     9721 tcttgcttca tatgggaatc aacacagttg acttaggcgg tacgccattt gatgtgaaag
     9781 taacagaagg gcagcaagtg acacctgata cgcttgtagc agaagctgac ttagaagcga
     9841 tcaaagcagc aggcaaagaa acatccatga tcgtcttggt aaccaatatg gatcgtgtaa
     9901 aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg atggatgtca
     9961 aagcttaata tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta tttttcagct
    10021 tcttcttttt tattatttat tgttttctgc taaaatctga taaattcgtg tcacatcaga
    10081 tggtgttccc cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt gtgcatattg
    10141 tttggctgtt agacaatact gattattgaa gtttttattt ccgctaccga ctacaccaag
    10201 acaaagttta tgattgtctt catgttccag atattcacgc atcgtttcgg tcaagatttc
    10261 tgtatcaccg ttatctaaac catttccgcc gtctaaataa gtaggaacga aagtaaaaaa
    10321 tggttctgtt tctttcgtaa aatcactatt ttcatggatt tcttttaaag taacttcagg
    10381 attgacagga tcttctgcat gcattttttc agcatattct gctagatgtt tggcaaaggc
    10441 acgcgtattt cctgaaatcg atatatataa aatgttcatg gatcatcctc caattctttc
    10501 cttttagtat agcatctttt ttaagttttt tggtggtgta atttggaaag ggactaggcg
    10561 ctttgcttgt ataatgagaa aaaagccggt agaattatat ttctattaaa gtttttatta
    10621 attaagtaaa aataaaaaag gtttgttata ctttttgata aaagcggttt tattttggag
    10681 gatgcttaca tggatacaga gcaacagaaa aaaggaattg gtttaattcc acttgctgcc
    10741 ttagtaattg gttcagcgat tggaggtggc gtatttggta ttatgacgga catctccgga
    10801 tcggctggcg gcccagctat tttcagttgg gtactcgtag gaacagcaat gttgatgctc
    10861 tccttgtcga tcaataatat caataaaaaa cgtccagatt taaaaggcgg gatctttagt
    10921 tacgcagaag aaggttttgg ccgtttcgct ggttttatca gcggttgggg ttactggctc
    10981 acatgctggt taggcaacgt agcttttgca acattgttga tgagcgcatt aggatatttc
    11041 ttcccagtat ttgaaggtgg gcaaaatctg gcatcgatcg tgatgagtac agtcttttta
    11101 tggggatatg catggctagt gaatcgggga gtcgaaaatg ctagtttcgt caatgctatc
    11161 gtcacattct gtaaattagt gccattattt ttatttattg tagtagctat cagcacgttt
    11221 tcgatccaac aattcattga taacttttct agcgacatta tattgaatgc gaatggacag
    11281 ccgatcccgt tttccagtca agtattgagt tgcatcatgg tgatgctttg ggtatttgtt
    11341 ggtattgaag gagcctctgt cgtaggttca cgagcaaaaa aacaatcaga tgttgggaaa
    11401 gcaacgattc tagggatatt cggcttgatt attatttacg tcttggtttc catgcttcca
    11461 tatgggacaa tgacgatgaa tcagctgcaa acgatcaagg cgcagcctgc tatgggctat
    11521 gtgtttgaaa tgatggttgg taaatgggga gcagtcgtga tcaacggcgg tttgatcatt
    11581 tctttagttg gtgtttggct ctcatggacg atcttaccga ttgaaacaat gggaaacatg
    11641 gctgaagacg gacttttgcc taaagcatgg ggaaaactca acaaaaatgg ggcaccaact
    11701 tatgctatcg tattgacgac gttatgtacg aatgtctttt tactttcatt attattcact
    11761 gacagtgctt ataactttgc ttacacactt ggtacagcag ctattttctt cacgtggctt
    11821 ttcttaggat tgtaccaaat gaaactttct tatcagcgga aagaatgggt ccaattttta
    11881 gtagggcttt tagcaagtgc ctttcaagta tgggctatgc tagtcgtagc ctttaatgaa
    11941 gtaatgcttg ttcttgttct tttcttgcca ggcatttatt tttacctcaa agcacgtaag
    12001 gagcaaaacg agcctgtaca acatatccac ttagataaga ttttacttat aggtgtgacg
    12061 atggtcggag tgattgcaat ggttttattt gccactggga tgcttcacgt atagaagtat
    12121 aaaagaagta taaagagagg ttgtgagcct gtcgtttatt caaaaacata ctaggaaagg
    12181 agctgtgaca caaatgttgt cacagctcct ttttcagaat aaacggtgtt taagagacag
    12241 ccaatcggca ataaggctga acgtctcttt cacagcctct tttttgatga gtcatcatac
    12301 attttttcgc ttaaatacga gatatcctaa tgctaagaac aaaacgatgt aagctaaatt
    12361 accgaatagc atttgattag tagaaagatg agtcaattgc ttcatgatct cttcgtttcc
    12421 aatttggttt tgcagattca acatactgat cggattccat ttgatccact cccatttttg
    12481 gatgagtgca ataaggatac cagatatgat tgacgaagca aaataaaaga cgattcctgc
    12541 tgagatggat gcgccggaac tattgataaa gcaagcaatc atcaacacta aacttaaaat
    12601 cagccataaa ccgatataac tacccaaagt ataagtgaac aagctttgga tcagtgtttg
    12661 accagtagaa accttctccg tgaaggaaat cgaagggaaa agaatcagtt tcatcaacac
    12721 agtgacaata atagctaaaa ggtataaata aacagaataa ataactaatg tgatccattt
    12781 gcttactaat atttctcctc gagaatactt tcgataaagg agatttttca atgtaccata
    12841 ttgtgcttcc atcgaaatga tcgtgcttgc agctgcaatc atgataaaga caatccaaga
    12901 tgtggcacta aaaagctgag cagtcatttc aactggatca atgatcgtcg tgtatttttt
    12961 ggctagaagt gctgttccta tgagcaaaac aactaataga acagataaaa cagcagttga
    13021 ctttttcttg atcaatttaa ataattcttg ttttacaagc aatgtcattt ttcttcttcc
    13081 ccctttactg aatccagaat ttccaataga gaagattcca aatcattcgt cagatgctgg
    13141 acgtctgtca catggatatt ttgtgcagac aatagctgga tcacggttcc taataattct
    13201 tcgttttctt ttaaaagctt gattttatca ggatcttcca aagtatatcc agcttctgtc
    13261 aagatcgttc gagcagctag atcatttgtt gtagagataa cgatgaaatc ttgcgctgtt
    13321 tggttcaatt cagccattgt acattgtttg atgatctttc cttgattgat caggatcagc
    13381 tcatcgacca ttttttcaag ctcgctgagt aagtgactgg agatcaagaa agaggtgccc
    13441 tgagtagcca agtcggtaat gatttttcgc acatctcttg ttgcttgtgg atccaaaccg
    13501 ttcatcggct catctaaaat aacgatagac ggatggttca gtaaggcaag agcaattccc
    13561 aatttttgtt tcattcccaa cgagtaggat ttcgcttttt tgtgaatgta cttttccatc
    13621 ttgagctgat tcacgatagt gtcgatagca gatacatcgt tggtttcaga gaataacttc
    13681 agatgatcat agcctgttaa gaatggatag atacccgggt attcgatcaa tgctcctact
    13741 tgttccaacc ctttatgact agagggagaa atctcatttt caccaataca gattgttcca
    13801 tctgagtaat ggatcagtcc taaaatagct ttcatgatcg ttgtttttcc agcgccatta
    13861 ggacctacta atccaacgat tttgccaggg gataagctaa aactgacatc gctgagggct
    13921 tgtttgtgac caaagttctt ggataattct tggaccgaaa gacttttcaa aaatattcac
    13981 tccttttcta tagatagtac aagcatacac ctgatgagtg gagaagtcgc taggtcattg
    14041 gacttatttc ttattttttc ataaaaaaat tgaatattaa ataaaaaggc ttattttttc
    14101 tgatcagtac ttattccaag tgtctagtgg ccgctagtaa tcttgatgcc aatgatactg
    14161 atgagtagca tccccacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc
    14221 acaccaatta tgaccgaacc aactgcgcca atccctgtcc agataggata agagagactc
    14281 atcggcaaac ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat gccaatcact
    14341 gtgtaaaaag aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag
    14401 aatacttcca aaattcctgc tatgattaat tccatccatg ccatacttac attcctcctt
    14461 aactataaaa aaatacacaa aaagaggaaa atcatccctt caaagaccta atggaatgat
    14521 catcctcttt atcctacccg gtggcagatt gtatatcttt ttctgtagta tagcacacct
    14581 atccagatat cggcaatctc atacttttta gtaaagtgta ccaagcactg acggatatat
    14641 cgttagcgga aaagaagatg aatgctttgc aatttcatag aaagaagttg ttatttatta
    14701 atcatttaaa aaaaacagta actagacctt atttctatat attatcgggc aataaattaa
    14761 aaaaagcttt accttacaat aagtaagata atttttgtga taaaaaaata acgaaaaata
    14821 tttaaaaata gtgtctattt cgtgagaaaa caactatatt ttttatgtgt tttgctttat
    14881 aattaaaaaa atattgtcat tttatggagg aaaatatatg gaaaattatc ttagcctttt
    14941 aggcgtcttg attgttattc tcgggtttgc atttaaatta gactcgatat tgatcgtaat
    15001 ggttgcttta gttgttacag ctttgactgg cggtcttggc attcaaggaa tgttagaact
    15061 tttaggtaca agttttgtaa acaatcgcgg gatggcgatt tttatcatca ttatgttggc
    15121 aacaggaacg cttgaaagaa atggtctgaa agaatcagcg gcttccttga tcaagcgatt
    15181 caagaaagta tctgctggta tgattgtaga tatttatggg atctttcgaa tgatctttgc
    15241 ggcttttaac gtgagttttg gtggagttgc tggatttgtt cgtccaatca ttttaccgat
    15301 ggcattgggg accatcgaat caaaagggtt agaagtccat cctaagcatg aagaagaact
    15361 aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc tttggccaag tactattcat
    15421 cggaggagca ggggcgttgc tagtccaatc aacactgaaa gatctaggat atgacgtaac
    15481 attgggacag cttgctttag tggaagttcc agtggctatc acggctttag ttgtggcaag
    15541 tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa tattatccag gaaaggggca
    15601 ataagatgga cttttttaca aatcctgata ttttattagg cgataaatta ctggagatcc
    15661 tttatatcgt gatgggactc gtattgattt acactggagt aagaaattta acagataaaa
    15721 ccaatcctca tcgttatggg tcagcctttt tctggacggt cttaggggta gttattgcag
    15781 gaggaagatg gcttccggca atcgtgaacg gtggcttgat ctttgcgatg accataccag
    15841 caattgctaa gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa tatatggaaa
    15901 aaatgtcaga caaactagga atgaaaattt ttattccagc tttgagtatc ggtgtctttg
    15961 cgatcttgtt tgctctattt accaatttag gtgctttagt cggcgtgggc gtcggtgttt
    16021 ttgcagccat gctaatcttg atgttctttt ctcgagataa tcgaccaagt acatttttag
    16081 atgatgcagc agatatgctg ggcacagtag ggccgttaag catgttgcca atgcttttag
    16141 cttctctagg tgcggtattt acaagcgctg gagttgggac agtgatttcc aatgcagtag
    16201 gtacgatcgt tccagaagga aatgtgaaca tcggcatcat catttacgct cttggcatgg
    16261 tgatatttac ggtaatcatg gggaatgcat ttgcggccat caccgtgatg acggttggga
    16321 tcggtgcacc attcgtattt agtcatggag cgaatccagc gttaatagga atggttgcat
    16381 tgacttgcgg tttttgtggg accctgctga caccgatggc tgctaacttc aatatcgtgc
    16441 ctgtagcgat gctggagatg aaagacaaat acggagtgat caaaaaccag ttgtttattg
    16501 cgttatttat gctagttttc caaattgctt atatgattct atttaaataa tagaagagag
    16561 gggttgtgac agaataattg tcgcaacctc ttctaacatt gtagtggaac actatttttt
    16621 tataagaata tattaagttt gatctgttat gttatttaaa ctattgtttc ctttatttac
    16681 gctgttcaag gaaaatggta tgataaaaga taaccatatg ttaaatcaat cggacggagg
    16741 aacaacagat gaaagtacta gtaacaggtt ttgatccatt tggtggggac aaagtgaatc
    16801 cagcctatga agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa atcatcaaag
    16861 tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc atccaacagc
    16921 accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca gtctcgttcg
    16981 aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga taatgaagga aatcagccat
    17041 tcgatacggc tttagaagaa aacggtccag ccgcttactt tacctcattg ccaatcaaag
    17101 caatgacaaa aaatgtacaa gatcatggtc taccagcgta catctcttat acagcaggaa
    17161 catttgtctg caatgatatc atgtatcgtt tgcttcattt gatcgaaaca caattcccgg
    17221 caattcgagg tgggttcatc cacgtgccat tctcacccga tcaagtgatc gatcgtccag
    17281 tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc tttgacttat gcagttgaag
    17341 cagcagtaga aaataaagaa gatatttccg gaaatgccgg aacaactcac taatttagca
    17401 agaaacactg gctggttgct aacgttatgt tagtagccag ctttttgtaa ttagaaagga
    17461 aagaaacagc cgtggaagaa tggcaatccg tctttgaaga atggtttcca aaagaaatca
    17521 gcaaaagcta tcccatcaaa atttctaaac aatacacaag cagtcaacgt tgggagatct
    17581 atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga tatctaatca
    17641 gctctcgttt tatggaagaa cattatttag ctgcaacaga ttgggtgttt tctgacttca
    17701 agatcaatcc gttttttcga acaaaacgaa gccagcaaaa attgtattgt gagtgtggta
    17761 gagaattaaa agtgcaatac atcgttaaat cgccaaaaac cggaaagatc ctaaaactag
    17821 gcatcaacca ctttgccgat cacctgcatg tctctccgac agtggcagct tcgatccatc
    17881 agggaatgac aaaagttgat ctggccttag acgaattatt atggcttaag cagaagaata
    17941 tcgattttcc tgaaggacta tggcaaaaat actgttttgt cttgtaccaa aatcgtcgga
    18001 tgaaacagcc atatttaccg gatataaaat tagctcagcg gctcgcagaa tttcgccaag
    18061 tagaaatgcc gatttacata gcggattatc aagcattgga aaatgaaatc aagaaaataa
    18121 gtgaacacat caatgggcag tcaaaaaagc gacagataaa aaaagaacta tttgacgact
    18181 ttgcagaaga attagtaaag gatgtagaag aattcttgat caactatcgc gcatttttac
    18241 gtaaagattg gcaatccatc gtatatgaag aagtgccagt acatcctaat gcttattttg
    18301 aaacatttat ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa gtcactgctc
    18361 agatggaata ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat ctatttattt
    18421 ggaagcaata ttgtcactat ggttttacag aagggttctt cgacagcatt cctcggatag
    18481 tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga agctatacaa tctgctgata
    18541 agaaagaccg tacagtctca aaagaaaaat ggcaactagt agtgaaagat atccaatctg
    18601 gaaatgtgca agagacaatc gacaaatgga aaggaaaaca ttatcgtttt actgaagctc
    18661 agaaacaagc gttggaatat tatcagaagt tagaagaatc actgcgtttc aatgatgagg
    18721 caagaaagta tctgaaggaa ttactataaa aaatagaggc tgggacagaa gcgtttaact
    18781 ccaagaaata agaa
//
LOCUS       DACEKQ010000262        12869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.089, whole genome shotgun sequence.
ACCESSION   DACEKQ010000262 DACEKQ010000000
VERSION     DACEKQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.089"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1818)
                     /locus_tag="IW671_003032"
     CDS             complement(<1..1818)
                     /locus_tag="IW671_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0899947.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDL"
     gene            complement(1815..2687)
                     /locus_tag="IW671_003033"
     CDS             complement(1815..2687)
                     /locus_tag="IW671_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0899948.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3029..3901)
                     /locus_tag="IW671_003034"
     CDS             complement(3029..3901)
                     /locus_tag="IW671_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0899949.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4029..4598
                     /locus_tag="IW671_003035"
     CDS             4029..4598
                     /locus_tag="IW671_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0899950.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            4665..5402
                     /locus_tag="IW671_003036"
     CDS             4665..5402
                     /locus_tag="IW671_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0899951.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5633..6901
                     /locus_tag="IW671_003037"
     CDS             5633..6901
                     /locus_tag="IW671_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0899952.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGFTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7388..7852
                     /locus_tag="IW671_003038"
     CDS             7388..7852
                     /locus_tag="IW671_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0899953.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            7836..8465
                     /locus_tag="IW671_003039"
     CDS             7836..8465
                     /locus_tag="IW671_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0899954.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            8819..10297
                     /gene="msr"
                     /locus_tag="IW671_003040"
     CDS             8819..10297
                     /gene="msr"
                     /locus_tag="IW671_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0899955.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10344..10790
                     /locus_tag="IW671_003041"
     CDS             10344..10790
                     /locus_tag="IW671_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0899956.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(10989..11504)
                     /locus_tag="IW671_003042"
     CDS             complement(10989..11504)
                     /locus_tag="IW671_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0899957.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11621..12823
                     /locus_tag="IW671_003043"
     CDS             11621..12823
                     /locus_tag="IW671_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0899958.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4016 a         2365 c         2627 g         3861 t
ORIGIN      
        1 aagatcaact aaagcaatca attgacctct tgcgactttt tgaccttctt ctacatataa
       61 agtgaatggt tcgcctttta agtcaacagt gttgatgccc atatgaagca agacttcgat
      121 accagcatct gtttggattc cgacagcatg tttcgttggg aaaatgtttg tgatcgtacc
      181 agcgattgga gagaagattt ctccattttc tggtaataca gcatagccgt cacccatcat
      241 tttttccgcg aatacatcat ctgaaacttc agtgattgga atcagtttcc catttgcgat
      301 agcgtaaata tcttgttcga tcgtttcacc ggcaaatgtt tctgttttaa ccacggtgtt
      361 tgtttgtgca ggtcgtgctg cagcaccacc atgtagtttt gccatctcat ctgcaacgaa
      421 ttgtacttct gtacctacga tgacttgcgc attttttccg tcgatgatct tcataccagg
      481 tacgccagtg gctttgattt tattttgatc gatcaggcct gtatctttga cttgcaaacg
      541 aaggcgtgta gtacagttat cgatcgaagt tacattgtcg gctccgccta atgcagcgta
      601 gattttgctt gcttgtgccg catatttatt gtctgaatta gtcgttgcag tatcgtcatc
      661 tgaaagatct gcttcctcgc ggccaggagt catcagatgg aattttttga tagcaaagtt
      721 aaagccaaag tagtaaatca ctgctgttac taatccttgg acgatcaaca tcaatggctg
      781 attcgcaatc ggatttttca aagataggaa gtaatcgaca aatccggcac taaaggcaaa
      841 tcctgctgtc caatggaaga atgcactcac tgctaaagaa atcccagtga aaattgcatg
      901 caagacatat aatggccaag caacgaacat aaatgaaaat tcaagtggtt ctgtcacacc
      961 agtaaagaat gaagcaaaag ctgctgctag catcaaggaa gctgtttttg ctttgttttc
     1021 tggacgtgca tttctgtaaa tagcatatgc tcctgctgga agcccgaaca tcatgattgg
     1081 gaaaaatcca gcttgataca ttcctgttac gccttttgtt ccttcagaag ccaaaaattt
     1141 accaatatca ttgattcctg ctacgtcaaa ccagaacacg gagttcattg cttgatgcaa
     1201 tcctgtaggg atcagtaaac ggttgaagaa gccataaaga cctgcaccac cccagcctaa
     1261 actcaaaatg aatttagcaa aagaaactaa agcagagtaa accggaggcc atacgagcag
     1321 tagcactgca ctgatcagca acatgacaaa ggcagccaag attggcacta gacgcttgcc
     1381 gctaaagaaa gataaagcca tcggtaattt tgcttcgtgg aaacgattat aaagagctgc
     1441 cgcaatcaat ccggcaataa taccaataaa tacgttactg atctgagcaa aagatggatc
     1501 aactgattct ggtttgatat tcaataaagt agctactgaa tcagttttta aaacatttac
     1561 tggaacttca aatgcgacca aacctgccaa ggcagcagca cctgatttgt ccactgacat
     1621 ccctagtgct aacccaacgg caaacagcaa aggtaattga cctaaaactg cgttaccgcc
     1681 agcttgtaaa aaatgagcga caatgtcgtt actataagat gcccaccagt ttccgatccc
     1741 tactagtaaa gcagcagcag gtaagaccgc aactggaagt tgaagggaac gtcccattcg
     1801 ttgtagatat gctttcaatt tttttcctcc tcatgattgt taacatttgt ttgtacgatt
     1861 gcgcttctta aacgctcgat atggatggcc aagtaaccca gttcttcttt cgtgatcgcg
     1921 agctggtatt ctttggtcag caacacacgg atttttttag aaatcttata gctttctggg
     1981 tattttcgtt tgaccatttc tacgatttcc gtgtctaaaa cagcatattg ttgcgcatgg
     2041 gttcgttgta gaagtagtcg taaatgattc accagtcttg aataattcag tgaaaatggt
     2101 tctgaatgga tatctgtttc caattctgat tccactaaat ggatgatgtc agatacaatc
     2161 gtcacttcac ggatactgcg atggttattg ttccgaccgc ttcgtccgct gtgaatatgg
     2221 attgcgatat atcctgcttc gtcataagta tatggaatcc caagctcttt tgtcagatac
     2281 tctactgccc attgagcaat actgaattca tcactgtaaa gcacttcgat ttctcttaaa
     2341 agtttgttgc ggatgacgat accgttatta atgttctccg cagaaaaagc aatgtggtcg
     2401 gtcaaagcga tcaatacatg ttcattcaac ttttccatca aaacagtttc cgcatgatca
     2461 atgatcgttt ctgctgcaaa taaaaagcgt tcatcaatct gattaagtaa attttgcaac
     2521 ttgatttgac cttctggttc catgacaaat atccgttcga catcacgatc aaaaatcaaa
     2581 tcatttcttt ttctgtcaaa tccaatccct tttccaatag ctactttttc ctgtccaccg
     2641 tcatctacca atactgcatt ttgattaaag atctttttga ttttcatgcg cgcacccttt
     2701 ttgaagattt ggttttcacg tttgctagac cagttgattg cggccaaaca aaaatagaca
     2761 cgaaaaacct cctagggaaa ccatgttcct cagagatttt tcatgcctag tatgatctag
     2821 tcacatgaaa cgaattattg ttaacgtaat caattttata tagacagtta tgttttgtca
     2881 attctttttt tataattata gtaatttact ggaattatca gaaataaaag gaagaaataa
     2941 attaagaaac ttatgttatt taatcgttgg atcttttgct tatcttattc gttgttaaaa
     3001 agctgaaagc gaaggaaaaa aactctactt aactgtattt ctccgttagg acatctaaaa
     3061 atttacgagc agcaggagaa agaggctggt cttttttcca aatgagattc agtgaagctt
     3121 gtaatggcgg atcgagtggg atgaatattt tttccgtccc ataagtattg atgatcccat
     3181 ctagagctaa tgcaccaaaa gtcccgttat cgaccatcaa agaagcattg tacaataagt
     3241 tatatgttcc gacgatcgat aactgatcta tgctgaaacc tagccattca gctagttgac
     3301 tgtccactga cgtttgccgg gaaatgatca aaggaacagt tttcagttcc tgcggatgga
     3361 tgacggtttg ctttgctaaa ggatgatttc gatgtatcaa aagtccccaa cgatcttggg
     3421 ctggcagtcg aaatgactgg tatttttgtt tgtttgtcgg ttcgattacg agtccaaaat
     3481 caagtaatcc gctatccagt ttgtctatga tatcatctgc attcccgcta tataaatgaa
     3541 actggatatc aggatgcgcc ttgatcaacg aatgaaggct atcagaaatg aaagagattg
     3601 ctttagtttc gcctccgcca atgtagattt ctccagagat gattgcgtca tttgttaaat
     3661 tgttcgccgt tttttctacc aacgacaaga tttcttttcc ttttgaataa agatatcgac
     3721 cagaatccgt caactgtatc tctcgggagc cccgagtgaa aagtgtcaca ccaagttctt
     3781 cttctaaatc tttcagttgt tttgagagag taggttgcgt gatatgcaga gattttgctg
     3841 ctcggctgat atttttttct ctagctactg ctaaaaaata cgtcagtata cgaatatcca
     3901 ttttttccct cctgttttta aaaaagcata ccactaagta tagttaaaag gaatagttaa
     3961 gtatgtttta taagtattag acatgataag ccgtccgtta taaagtaagg ataaaaggag
     4021 ggagcaaaat gacaagcctc atcgaaaaac aaatcgtcaa taaagagatt cgagtaacgg
     4081 atccgctctt tgaagtgatc catcagatcc aagctgaaaa cgaagagcct ttagcagcgc
     4141 tgaatactgg gtaccatgaa ccagcagata tccgcaaacg gctagaaaat atcattagtg
     4201 acaaagtaga tgatacggtg actgttctgc tgccttttta tacagatttc ggcaaacata
     4261 ttagtatagg gaaaaatgtc tttatcaatc gtcaagtgat gttcgttgac ttaggtggca
     4321 tctgtttaga agattcagta ttgattggtc ctcgtgtcaa cctcatcacg gtgaaccatc
     4381 taaccgatcc aaaagaacga agaggcttgt cagtaaagcc aatccacatc aagaaaaatg
     4441 cttggatcgg agcaggagca actattctgc caggcgtaac gattggcgag aatgccattg
     4501 ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa taccatcgtt gcaggaatcc
     4561 ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac agtaagatga aataagagat
     4621 agtgagtata agcaagagat ttttcaaaac aggaggagaa tagaatgaat gatttatcag
     4681 aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg tgaagcaaca acgaagctat
     4741 tagcagaaaa aggggctaaa ctagttatag ctgcacgccg agaagatcgc ctaaaagcaa
     4801 taaaagaatc tttaccagaa gctgagctat atattcaaac agcagatgta agagattttg
     4861 cacaagtcca agcagttatc gatttagcga tggaaaagtt cggtcgaatc gatgtattgt
     4921 acaataatgc aggaatcatg ccaaccgctc cattagttga aggtcaccgt gatgaatggc
     4981 aaaatatgct tgatatcaac atcatgggtg tgttaaatgg aatttccgcc gtacttccaa
     5041 tcatggaaaa acaaaaatct gggcatatca tttcaactga ttctgtggcg ggacatgttg
     5101 tttatcctga ttccgccgtt tattgcggaa caaaatttgc tgtccgtgca atcatggaag
     5161 gattacgtca agagcaacgg caaaataaca tcaaatctac gatcatctct ccaggagctg
     5221 ttcaaactga attatatcaa accatcagcg acaaaaaggc tgctcttgaa cttcatgaag
     5281 cgcaaaaaga atggggattg acctccgaag atatagcttc tgctgtagca tttgcgatag
     5341 aaacgccaga ccggatgtct gtcagtaaca tgattatccg tccgacgaca caggaagtat
     5401 aatccataac ggataatatc caaagagaat tacttgttaa atagttattc aaacgaccat
     5461 caatttaagg cgtgaatgga gaagaatatt cttcatttac gtctttttat gtagaaaaag
     5521 caaatttatc aaaaagaaac aaatgttatt taaatatgga cattgacttg cttttacttt
     5581 tttagttaca ctgttattta ggaaaagttt cataattcaa aggagaaaaa agatgaaagc
     5641 aaagaattta ttatttagcg ttattctttt aggaaatcta gcagttgcca acacaacagt
     5701 gctagctgaa gaatcgacag aaactgtcgc aactcagaca tcgagttctt cagaaacagg
     5761 aattgaaaac gatacagaga gtactttgca gtcagctact gtctcagaaa atacaatttc
     5821 aaccgaagaa ccaacaactg aaacaacaga acaaacacaa accgaagaca gtacatcagc
     5881 agaagctcag aacaatccag agaatgcaga agaaatcacg aaccaattga cagatgggca
     5941 aacattgaca catacccatc aagcagattt gtctgaattc gaaaactata ctggctatgc
     6001 tttttatgct cgctcatctg ctaattctca acaagcgttc attgatagta ttgcctcaac
     6061 tgcacaaagt ttggcaagtg ccaatgactt atatgcatca gtcatgattg ctcaagccat
     6121 cgttgaaagt ggctggggga acagtacttt ggcttctgcc cctaattaca atctatttgg
     6181 gattaaagga agctataatg gccaatctgt tacgatgcct acttcggaat atgtgaatgg
     6241 gcaatggatt acggtgaatg ccgcttttag aaaatatcct tcgtacaaag aatcattgca
     6301 agataacgta acggtattga agacgacttc ttttcagcca ggcgtttact actattccgg
     6361 tgcatggaaa agcaatacga actcttacaa agatgctacg gcttggctaa caggtcgtta
     6421 tgccactgcg cctaattatg gttttacatt gaacaatgta atcgaaacct ataatctgac
     6481 acagtatgat acagcaccaa cttcaacttc tccgatgtat cgtctgtata accgtcatac
     6541 aggagaacat ttatatacat tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg
     6601 ggaatatgaa gggattgctt ggcaagcacc aaacagcgga caacctgtct accgtttata
     6661 caatccttac agtggagacc atcattacac aatggcacaa tctgagatca actctttaac
     6721 aaaaatcggt tggcgttatg aaggtctaag tttttattct ggtggttcca agccaattta
     6781 tcgactattt aatccaaatg aaaaaactgg tacccatcac tataccttga gtgctaagga
     6841 aagagattgg ctaacaccga tgggctggag atatgaagga ataggatttt atggttatta
     6901 aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa gaacagccaa acaaaaacag
     6961 attttcatgt tttttgtttg gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt
     7021 ttactcaaaa aaggttgtga caacatcttg tcataacttt tttattactg tttaagcata
     7081 ctgatccaat ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg
     7141 cggtgtaaaa ttttctactg cagaagagct atcttctata gtttgataag aatcaataat
     7201 cttggtttta gcgttgtgct ttctttatca ttttttccgt tcattctaca aaaaatttct
     7261 tttctctata cttttctatt gagagcgttt tttttattac acagctgatt ctttgacttt
     7321 tttctttttt tattctatta ttgacaagtc aatcgttgac gagtcaatta ttagaaagga
     7381 ggaaattatg tctttagaag agattagtca ccttttatat caaatcaaat tagccgatca
     7441 aaaaatcgtc cagatctttg aagaaaatat cggtataagt cttactagat acgagatcat
     7501 gatgagactg aaagaagaaa atccattgct acaaagcagt ttgcaagaaa agttgcagat
     7561 tgatcaagga gcagttactc gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg
     7621 tcagagaaat cctcagaaca accgggaagt gtatgtagcg ctaacagaaa aagcccagaa
     7681 gaaaatcgag cattgtgaaa tggaccataa agatacaggt tccgtgctat ccccggcttt
     7741 cagcgatgta gaaatgaaac aatttctaac acttttagat aaattgaata cccaactttg
     7801 caatataaca acagaaacga aagaggttaa taataatgga acaaaaaaat gatttttcaa
     7861 caattatgac tggtagaaga tccgttcgcg tatatgatga aacatacaaa atcccacacg
     7921 aagaaatgct ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc aatatgcaac
     7981 catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc
     8041 gtttcaatac gagacaaaat gatacttctt ctgcaatggt catgattttt ggcgatatgc
     8101 aatgttacga atatggcgaa gaaatctata accaagctta cgaatcaggg aagatgccaa
     8161 aagaagtcag agatcaacaa ttagctgcga tcattcctta ttacaagagt ttttcaagag
     8221 aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag
     8281 ttgcgcgtga tcatggctat gaaacaaatc caattggtgg attcgaagca gatcagttgg
     8341 cagaggcatt tggtttggat aaagaccgtt atgttccagt tattatttta tcaataggaa
     8401 aagcggtaga agaaggttac gagtctgttc gtttagcacc agaaaagatc actacgttcg
     8461 aataaaaggt tttcagttac tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa
     8521 cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt
     8581 gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat
     8641 gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt
     8701 catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt
     8761 tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat
     8821 ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact
     8881 atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg
     8941 tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa
     9001 aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa
     9061 ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg
     9121 gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga
     9181 acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact
     9241 gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt
     9301 tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc
     9361 ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga
     9421 agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa
     9481 acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga
     9541 tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa
     9601 aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca
     9661 aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca
     9721 aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc
     9781 tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga
     9841 acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata
     9901 tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca
     9961 aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga
    10021 aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc
    10081 tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga
    10141 tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt
    10201 tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg
    10261 gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg attttcgtga
    10321 caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata atttttacaa
    10381 caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg ctgcatcaag
    10441 atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg gctttcattt
    10501 tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc
    10561 cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta ggaatggtga
    10621 catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa ttattctctg
    10681 cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag ttgatcatcg
    10741 ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga tggaacactg
    10801 ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc agttctagaa
    10861 aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt cttttccaac
    10921 tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag aaaggttctg
    10981 tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca ccattggagt
    11041 tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc
    11101 ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt gttcagctgt
    11161 tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga aaaagcatag
    11221 atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt gatccattcc
    11281 tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga gtgctcgtct
    11341 ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt atagatctga
    11401 ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc agaacgtggt
    11461 ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat cctacctctc
    11521 gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg catatttaca
    11581 tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat tggagaatca
    11641 aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag cgctagttgt
    11701 agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa tcttaaaaac
    11761 gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta ctagttacat
    11821 gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga tcggtttgaa
    11881 aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg gcggattatc
    11941 tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga atgctttatt
    12001 tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag aaaaagcaat
    12061 catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag tcggtggggt
    12121 cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga tgttttgcgg
    12181 ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa aagtaggcct
    12241 ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt tcagttggac taatcgcatt
    12301 gttatataat ttcggattct ttactttact tgcgtatgcg ccatttttat tgattggcta
    12361 ttctgaatta gaagtcggtt ttgtattttt tggctggggt gtgcttctgg ctattgcttc
    12421 gattttcgtt gcaccggctc ttgagcggaa gtttacgact tatcggacaa ttttagcagc
    12481 atttgtctta ttcattattt gtttgctgtt attaggagct ggaacttcta tgccagtcct
    12541 tgtaccgatc agcattgtgt tagcaggatt tttccaagga attatcaata cactgttgac
    12601 gacgattgct atggaaatac caggtctgca acgaaacgtt gcttcgtcca gctatagttt
    12661 tgtccgcttt ttcggtggag ctttagcacc atttattgca ggtgtgattg gtgaacatat
    12721 tggtgaaagc tatgcgtttt attttgcagg tgccattgtt cttatcagtg tatgttgtat
    12781 catttatcac cgtaattatt ttgctacaaa tgaagaagaa taagaaatca gaggttgtga
    12841 aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DACEKQ010000263         7921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.118, whole genome shotgun sequence.
ACCESSION   DACEKQ010000263 DACEKQ010000000
VERSION     DACEKQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.118"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW671_003044"
     CDS             128..1564
                     /locus_tag="IW671_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0899959.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW671_003045"
     CDS             1753..4200
                     /locus_tag="IW671_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0899960.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW671_003046"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW671_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0899961.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW671_003047"
     CDS             complement(5690..6793)
                     /locus_tag="IW671_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0899962.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW671_003048"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW671_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0899963.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2646 a         1356 c         1723 g         2196 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 cggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 c
//
LOCUS       DACEKQ010000264         6749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.128, whole genome shotgun sequence.
ACCESSION   DACEKQ010000264 DACEKQ010000000
VERSION     DACEKQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.128"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            111..194
                     /locus_tag="IW671_003049"
     CDS             111..194
                     /locus_tag="IW671_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0899964.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="IW671_003050"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="IW671_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0899965.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="IW671_003051"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="IW671_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1649..2314
                     /locus_tag="IW671_003052"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="IW671_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="IW671_003053"
     CDS             2350..2595
                     /locus_tag="IW671_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0899966.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="IW671_003054"
     CDS             2698..3567
                     /locus_tag="IW671_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0899967.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="IW671_003055"
     CDS             3548..4282
                     /locus_tag="IW671_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0899968.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5227
                     /gene="ant(6)"
                     /locus_tag="IW671_003056"
                     /pseudo
     CDS             4315..5227
                     /gene="ant(6)"
                     /locus_tag="IW671_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5224..5766
                     /gene="sat4"
                     /locus_tag="IW671_003057"
     CDS             5224..5766
                     /gene="sat4"
                     /locus_tag="IW671_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0899969.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5859..6653
                     /locus_tag="IW671_003058"
                     /note="aph(3')-IIIa"
     CDS             5859..6653
                     /locus_tag="IW671_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0899970.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2365 a         1015 c         1407 g         1962 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgaacgtat
     4381 tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag atgaatttca
     4441 ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta atgatgactg
     4501 gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg aattattccc
     4561 acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata aaattgatct
     4621 taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat taataaaggt
     4681 tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata tagattatca
     4741 tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt ggaatgtaac
     4801 accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg atcattttaa
     4861 tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca tcgaaacagg
     4921 ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat ccgaggattt
     4981 gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat gggaagcatt
     5041 atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa ggcttcatta
     5101 tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt ataaaaaata
     5161 cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag agaggcggga
     5221 acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc cagcgaacca
     5281 tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa
     5341 ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag
     5401 gaggcagatt gccttgaata tattgacaat actgataaga taatatatct ttactaccaa
     5461 gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta cgcttatata
     5521 gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat
     5581 atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac
     5641 aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat
     5701 actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg
     5761 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
     5821 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
     5881 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     5941 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     6001 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     6061 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     6121 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     6181 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     6241 cggattgtcc ctatacgaat agcttagaca gccgattagc cgaattggat tacttactga
     6301 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
     6361 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     6421 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     6481 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     6541 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     6601 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
     6661 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctggctc
     6721 tttgtcaaat gatgttggta aagagaaat
//
LOCUS       DACEKQ010000265         6539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.131, whole genome shotgun sequence.
ACCESSION   DACEKQ010000265 DACEKQ010000000
VERSION     DACEKQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.131"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            391..957
                     /gene="amaP"
                     /locus_tag="IW671_003059"
     CDS             391..957
                     /gene="amaP"
                     /locus_tag="IW671_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0899971.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            970..1158
                     /locus_tag="IW671_003060"
     CDS             970..1158
                     /locus_tag="IW671_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0899972.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1171..1728
                     /locus_tag="IW671_003061"
     CDS             1171..1728
                     /locus_tag="IW671_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0899973.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1760..2002
                     /locus_tag="IW671_003062"
     CDS             1760..2002
                     /locus_tag="IW671_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0899974.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2343..3557
                     /locus_tag="IW671_003063"
     CDS             2343..3557
                     /locus_tag="IW671_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0899975.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3588..3806
                     /locus_tag="IW671_003064"
                     /pseudo
     CDS             3588..3806
                     /locus_tag="IW671_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4225..4698
                     /gene="dfrF"
                     /locus_tag="IW671_003065"
     CDS             4225..4698
                     /gene="dfrF"
                     /locus_tag="IW671_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0899976.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            4825..5481
                     /locus_tag="IW671_003066"
     CDS             4825..5481
                     /locus_tag="IW671_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0899977.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            5663..6193
                     /locus_tag="IW671_003067"
     CDS             5663..6193
                     /locus_tag="IW671_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0899978.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         2270 a          964 c         1362 g         1943 t
ORIGIN      
        1 gaatagtata aaccaaagaa gtgaataaga aaaatgataa tataaaaata aagtaagaac
       61 gcattaaagt aactaaagtt atttattgta ttttttaatt ctccgaaaaa atttaataaa
      121 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
      181 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      241 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      301 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      361 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      421 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      481 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      541 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      601 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      661 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      721 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      781 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      841 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      901 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      961 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
     1021 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
     1081 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1141 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1201 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1261 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1321 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1381 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1441 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1501 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1561 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1621 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1681 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1741 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1801 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1861 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1921 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1981 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     2041 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
     2101 ctttattcct tatgaaaaaa tatctttaaa aataaaaaaa gacgaaaata aacggttttc
     2161 tataaaaaga agaccgttta ttttttgtct ttatttgtgt gaaaaacgaa aaagagcaca
     2221 acacatcaag cttttattaa ttgtctgtct tttataaatg caagtattaa ttcaaaaaaa
     2281 cagaagaaaa aaacaactac accttctttt atactcgtaa tcgtagaaag gaggcgaaaa
     2341 aaatgaatac attacctatc aatacaggag atacagcatt catgatttta tgtacagcaa
     2401 tggtttgcct gatgactcct ggtctagctt ttttttacgg cggattagca cggaagaata
     2461 atatactaac gatcatgtct caatcttttc tctccgttgg gataacaacg attatgtggg
     2521 tatttggtgg attcggattg gcctttggac gggatatcgg cggtgttatt ggaaacccgg
     2581 cagatttctt tttgatgaga catgttacaa gcttccccaa catgtttcat ggtgcaacga
     2641 ttccattttt aatgttcttt ctttaccaac tgatgtttat cgtgattacc gttcctttga
     2701 tgacaggagc ttttgcaggg agattgaatt taaaaggcta tattatactt gttatttttt
     2761 ggaatctgct gatttatttc cctgtttgcc actggatatg gggtggcgga tttttggatc
     2821 agatgggatt tagagatttc gctggtggag cagtcatcca cacgacagct ggattcggct
     2881 ctttagcatg tatcctcaca cttggtcaaa gaaagattcc tttaggagaa agaagtcagc
     2941 atagcagtct tatggcagct gctatcggta caggactgct ttggtttggc tggttcggtt
     3001 ttaattccgg tggtgcactt cgagcagatg aacaagcagt caatgcattt ggttcaacat
     3061 tcatcgctct tgcatttgcg atgattactt ggctgatcat tgcaaaagta aaaggaaatg
     3121 gctttgattt tgtcgacgta ctaacaggat ccgttgctgg attggcagcc attacgcctt
     3181 gtgccggtta tgtagaagca aaaagtgcca tgctgattgg gatcattgcg ggtatcgtct
     3241 gtcatgctgc tgttgatttt cgtaaaaaga aacaatggga tgatgcactt gatgtgtggg
     3301 gtgttcatgg catgggcgga ttcacaggta cgattctgat agggatcttt gcatctaaca
     3361 gccatttact aacgaatcca agcagctggt acttcttagg gattcaagtg attggtgtca
     3421 tcattactgc cgtgtatgcc tatgtgctga caacagtgat tttaaaatcg gcgatgcact
     3481 ttacaacgat cactactaca aaagaagaac aagaagaagg attggacttt ttcttctttg
     3541 gcaacaaact caattgaaac aaattcaaat agaaaagagg aagattcatg caacaaagca
     3601 aaacgaaaat tcctgcaaaa aaattaacgt tctttggctt tttggctatg accatctcaa
     3661 tggtcgtttc attatatgaa tatcccacat tcgcgacctc aggcttcagc ttagtatttt
     3721 tcttgttatt aggcggtttg ctatggttta tccctgtggc attgtgcgca gcagagatgg
     3781 caacggtgaa aggatgggaa aaaggtgttt cccaaacaaa aagatattaa gaaacagccc
     3841 atgtttacct gcttgcaaac aaaagaatat tgctactaga ctaagagata ctacataagt
     3901 gtgttggtat ctcttttttg ttgccgtgaa acagcagact atttcatttc tgcttgatta
     3961 ccttaaaact tattagtcta tggtttgtca agggcttgtt gcgtgagaaa tgctgaatat
     4021 caccctttac aaaacatagg ctgataagta acttttcaaa agcagaaaag aaatggcaat
     4081 gcttataaca atatgatata atgcagatga agacaaaaaa ggaatttgtc gagctggtta
     4141 gttcgagata attttgagtt tgttaagatg tggtgcactt gatggaatca caggtacgac
     4201 atcatattat ggaggatgaa atagatgata ggtttgattg ttgcgaggtc aaagaataat
     4261 gttataggca agaatggtaa tataccatgg aaaataaagg gagaacaaaa gcaatttaga
     4321 gagttaacaa cgggtaatgt ggttattatg gggcgaaagt cttatgaaga aatcggtcat
     4381 ccgttgccta atagaatgaa tattgttgtt tccaccacaa cagagtatca aggagataat
     4441 ttagtttcag ttaaatcatt agaagatgca ttattattgg ctaaaggacg agatgtatac
     4501 atatctggtg gatatggact atttaaggaa gctttgcaaa tagtagataa aatgtatatc
     4561 acagaagtag atttaaatat tgaagatgga gatacattct ttccagaatt tgatatcaat
     4621 gattttgaag ttttgatagg ggaaacactt ggtgaggaag tgaaatatac gagaacattt
     4681 tatgtaagga aaaaatgaat tgagtaaatt ttggatttag ggtgttttca taaatatatt
     4741 gctttttatg catatataaa ttgttgtaag acaaattcca gtttgtatgg ggcgataaaa
     4801 tcagaatttg agagaggatt gaatatgaat aaggaatggt ctgaacttaa taaaacaatg
     4861 caggcacaaa taaaaaagaa agatacttat aagaggggta ttgatacttt acttacttta
     4921 cgaagtcagt taatacaaac cttagtatct ttcaaagaag aattatgcag agaagatttt
     4981 aactcaatcc ccttcataaa cgctgatggt tatcatagta agacgattgc ttattctatt
     5041 tggcatattt tccgcattga agatatagtt gtgcatacag tgataaatga agatgaacaa
     5101 gtattttttg caggcaatta tcaagagcgt atcaattcac ctatcattac aacaggaaat
     5161 gaactcatga aacagcagat tgcagacttt tcaaaacagc ttaatctgga agaattatat
     5221 ttatatattt tcgaagtatg ggaaagcact gagaaaatgc ttgaacggtt atcttatgat
     5281 gaattgaaaa gaaaaatacc gaaagaaaga aaaggatact tagaatcgtt gaatgtagta
     5341 aacgacaatg aaaaagcaat ttggctgatt gattattggt gtaataaaga tatttgtgga
     5401 ctaattcaaa tgccgttttc aagacattgg attatgcaca cagaagcctg tttgcgtata
     5461 aagaacaaga tacattcata ggcaaaaatt tcagtttgta gaactaagga aattggaatt
     5521 taaggaggaa taaagtatgg ttagtgattt tataagagca gcatttccat ggattttaat
     5581 gggtttattt gtagcaatca gttgttccct tatgagtaaa aaagagaaat aaattcctgt
     5641 ttgtatttat aaaagagaaa aaatgagaaa agtagtttta tttattgcca tgagccttga
     5701 cggatatatt gcggacggta atggtggagt agcttggcta aatggtcatg gaaatgacaa
     5761 tgaaaacatc gacacttata cagaatttac caaagatata gatactgtct taatggggtg
     5821 gaatacttct catcaggttg ttactgaact ttctccgcaa gaatgggtat ataataaatt
     5881 tacaacttat gtattaacac ataaagagtg caattcttcc caacaaattc attttacaag
     5941 tgaaaatcct gttttattat tggaacggct aaaacaagaa gcaggaaagg atatttggat
     6001 ttgtggcgga gcaaatcttg ttcagcagtt ggtaagcaaa aatatgattg ataaatatta
     6061 catttctgtt attcctactc tgttaggaaa tggggttcgt cttttgggca acatagacag
     6121 ggaaataaag ttaaggcttt gtaaaacaca gacttataac ggaataaccg atttgattta
     6181 tatgcgtaga taactagaga gaaacagaaa agctgttaaa ccgacggagc gatccgacca
     6241 cgttggcggg aaaatcctta agatttttcc gccatgcttg caagggggcg gttcgcttcg
     6301 gcggttcggc gagcttgcga gccgttcaaa gcccccgtta tctaacaagg ggggcacaaa
     6361 aaacaagaaa cactatacac taacaagcgg aaaacgctgt atttataagg caaaacccca
     6421 tttttacagt agtgatataa attgtaccta tatcactatg aaaattgaat agtaaatatg
     6481 agaaagacag ttgatttcaa aaaataggct ctttgtcaaa tgatgttggt aaagagaaa
//
LOCUS       DACEKQ010000266         4302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.154, whole genome shotgun sequence.
ACCESSION   DACEKQ010000266 DACEKQ010000000
VERSION     DACEKQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.154"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            67..978
                     /locus_tag="IW671_003068"
     CDS             67..978
                     /locus_tag="IW671_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0899979.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1131..1616
                     /gene="vanZ"
                     /locus_tag="IW671_003069"
     CDS             1131..1616
                     /gene="vanZ"
                     /locus_tag="IW671_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0899980.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1903..2232)
                     /locus_tag="IW671_003070"
     CDS             complement(1903..2232)
                     /locus_tag="IW671_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0899981.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2537..2866
                     /locus_tag="IW671_003071"
     CDS             2537..2866
                     /locus_tag="IW671_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0899982.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2856..3143
                     /locus_tag="IW671_003072"
     CDS             2856..3143
                     /locus_tag="IW671_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0899983.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3422..3985)
                     /locus_tag="IW671_003073"
     CDS             complement(3422..3985)
                     /locus_tag="IW671_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0899984.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1503 a          656 c          722 g         1421 t
ORIGIN      
        1 ctaagatttc tctttaccaa catcatttga caaagagcca ctatatagtt aaggaggaga
       61 ctgaaaatga agaagttgtt ttttttattg ttattgttat tcttaatata cttaggttat
      121 gactacgtta atgaagcact gttttctcag gaaaaagtcg aatttcaaaa ttatgatcaa
      181 aatcccaaag aacatttaga aaatagtggg acttctgaaa atacccaaga gaaaacaatt
      241 acagaagaac aggtttatca aggaaatctg ctattaatca atagtaaata tcctgttcgc
      301 caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac atgacgaatt aataaatgga
      361 tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa tagcacaaaa attttcagag
      421 atggtcaatg atgctgtaaa gggtggcgtt agtcatttta ttattaatag tggctatcga
      481 gactttgatg agcaaagtgt gctttaccaa gaaatggggg ctgagtatgc cttaccagca
      541 ggttatagtg agcataattc aggtttatca ctagatgtag gatcaagctt gacgaaaatg
      601 gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt ggaaatacgg gttcatttta
      661 cgttatccag aggacaaaac agagttaaca ggaattcaat atgaaccatg gcatattcgc
      721 tatgttggtt taccacatag tgcgattatg aaagaaaaga atttcgttct cgaggaatat
      781 atggattacc taaaagaaga aaaaaccatt tctgttagtg taaatgggga aaaatatgag
      841 atcttttatt atcctgttac taaaaatacc accattcatg tgccgactaa tcttcgttat
      901 gagatatcag gaaacaatat agacggtgta attgtgacag tgtttcccgg atcaacacat
      961 actaattcaa ggaggtaagg atggcggaat gaaaccaacg aaattaatga acagcattat
     1021 tgtactagca cttttggggt aacgttagct ttttaattta aaacccacgt taactaggac
     1081 attgctatac taatgataca acttaaacaa aagaattaga ggaaattata ttgggaaaaa
     1141 tattatctag aggattgcta gctttatatt tagtgacact aatctggtta gtgttattca
     1201 aattacaata caatatttta tcagtattta attatcatca aagaagtctt aacttgactc
     1261 catttactgc tactgggaat ttcagagaga tgatagataa tgttataatc tttattccat
     1321 ttggcttgct tttgaatgtc aattttaaag aaatcggatt tttacctaag tttgcttttg
     1381 tactggtttt aagtcttact tttgaaataa ttcaatttat cttcgctatt ggagcgacag
     1441 acataacaga tgtaattaca aatactgttg gaggctttct tggactgaaa ttatatggtt
     1501 taagcaataa gcatatgaat caaaaaaaat tagacagagt tattattttt gtaggtatac
     1561 ttttgctcgt attattgctc gtttaccgta cccatttaag aataaattac gtgtaagatg
     1621 tctaaatcaa gcaatctgat ctttcataca cataaagata ttgaatgaat tggattagat
     1681 ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa accggtgata
     1741 aagtaaaaag cttacctaac actatagtaa caaagaaagc ccaattatca attttagtgc
     1801 tgaggaattg gtctctttaa taaatttcct taacgttgta aatccgcatt ttcctgacgg
     1861 tacccccaaa ctaagatttc atagatagat tttctgataa ctttaaaaaa cacgaatagc
     1921 ggaaaggatg tcttcattcg ctaaagagta ataaactact tttccatctt gacgtgattt
     1981 tgccattcca tgttttttta aaaaacgtaa atggtgtgac gttgtagcga cactagcagt
     2041 aagtatttct gctaaatcac agacgcacat ttccttatag gtttctaaag catagaaaat
     2101 tttaattcta gaaaaatcgc tgaaacattt ccctaacaca agtaagtttg aaaaatcttt
     2161 ttgatccaat ttatttttta ttccatttac tttttcttta tgtacgacag tcacttggca
     2221 aacttttttc attcatctca cctcacattc aaacgattat ttgaatgaag tatacctatt
     2281 tgatcactct tttgtcaatc taccttaatt ccaatacact attataagca atttcatgta
     2341 ttgaagtaaa cttacttgca agtgcgtttt tgattccaag ttaaaaacat attattattc
     2401 tacataattt cctaatcaaa taaaactcct ttgaaaattg ttctaaagga gttttattat
     2461 tttgtatttc tactaaaaat agcgtacaat gtaattacta agtaattacg aaatgtaaaa
     2521 taatggagga aataatatga ttacaaaaac aagaaaacaa ggaaattcta ttatgttaac
     2581 ggtccctaaa gaattcgatg tgcccaatgg tgttgaagta gaagctaagc ttgtagagaa
     2641 tggtatcctt tatgagtttg tagagcctaa aaaagaattt tttgatttta gtgaagatat
     2701 tttggctgat attctttcag aaggttatag caaacaagag atcttaaaag aatttagaaa
     2761 tagaaaaaat gcgttaactt ctgcatttaa atctattgct gaagatactg ttgcaaattc
     2821 taaaccgatg acaaaagagg agttggcagc agaaattggc ttatgagatt ctaccttcta
     2881 aacacgtaat aaagtatcta aaaaaattaa aagagaaaac attaaaagaa caatttttaa
     2941 cgataattta tgatgagata gcagttcgtc ctcattcggg agaacaaaaa acgggtgatt
     3001 tatcaggaat ttgggctatt ggattcaagt atgcaggtac gacatatcgc gttgcatatg
     3061 aaattaaaga taatgcagtg atacctatct tactatgtgg aacgcatgaa aatttttatg
     3121 aacaattaaa aaagattagg taagaaccca atgtttcaac ttgagaggat agacgacact
     3181 ttaaaagata atttttaaca gctgagagtt gtaataccct ataattttaa accactaacc
     3241 tatatattta tgaaaaatag aaaaaattaa aacgcatttt aagcctcttt caaattaaaa
     3301 gcttcatcct tatgcacaaa gagggcttaa aatgcgttat tttatattgt tttttagtaa
     3361 taataaaaaa atcactctaa ttctcccata aatattatag attcctaaag attaaaatag
     3421 attattccct attgtctttt aagtagcggt agagtgttgc cttgctaata ccagtcattt
     3481 ccgtaatttc ttttacgcta taaacctgac ttttatacaa ttttactgct cgatcaacat
     3541 ctttctggtt cactcgtgga cggccacctt tacgacctct cgcacgtgca cttttcaaac
     3601 cttctttggt acgttcaaca attaaatcac gttcgaactg actaaatgct tggaagacag
     3661 tcatcattaa gcgtccgtga ggcgtgctgg tatcgaagtt ttctttcaag ctcaccagct
     3721 taacatggtg ttcttcaaaa taggtaacaa ggtcaatcaa atcctttgta ctacgaccca
     3781 atcgagaaaa actttctaca attaccgtat ctcccggacg aattttatct tttaaacgat
     3841 ttagttgggg acgaaccgct ttggttcccg tcattttctc agttaggatt tcattacaat
     3901 tttgttcatt gagtaagtct aattgacgat ttagttcttg tgaacgtgta ctcactcggg
     3961 cataaccata aatgtatcca ctcatgcttt tcttcctttc gtactttaat tctggcttga
     4021 ttgtatcata aaagggagtt atagatacat agttaatgca acaagttatg atacaaaata
     4081 tgacctcttt tatttaataa aagaggtcat ttcattttgt ctcataatcg attgtttttg
     4141 atacgagtgt gtatacgaaa taaacaaaaa aatcttaagt aaatttctag atacctaatt
     4201 taatcctaac tagtgtatcc taaagttaat aacgagttta tcacaatgaa aattataaaa
     4261 tgctcccata ttagatataa catgcaatat ttgaattaga ac
//
LOCUS       DACEKQ010000267         3297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.172, whole genome shotgun sequence.
ACCESSION   DACEKQ010000267 DACEKQ010000000
VERSION     DACEKQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.172"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW671_003074"
     CDS             245..1213
                     /locus_tag="IW671_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0899985.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW671_003075"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW671_003075"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0899986.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW671_003076"
     CDS             2243..2851
                     /locus_tag="IW671_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0899987.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          980 a          632 c          795 g          890 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag gctctatact ttttgatgtt ggtggatgac accagca
//
LOCUS       DACEKQ010000268         2242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.191, whole genome shotgun sequence.
ACCESSION   DACEKQ010000268 DACEKQ010000000
VERSION     DACEKQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.191"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..148
                     /locus_tag="IW671_003077"
     CDS             <1..148
                     /locus_tag="IW671_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0899988.1"
                     /translation="LIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKLS
                     EVNN"
     gene            362..1057
                     /gene="vanR"
                     /locus_tag="IW671_003078"
     CDS             362..1057
                     /gene="vanR"
                     /locus_tag="IW671_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0899989.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1035..2189
                     /locus_tag="IW671_003079"
     CDS             1035..2189
                     /locus_tag="IW671_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0899990.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          757 a          409 c          475 g          601 t
ORIGIN      
        1 tttgatagta gatttaaaac tttgcaacag aaccaattat gcggtaaagc tatataaaga
       61 aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata
      121 caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt
      181 taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt
      241 tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat
      301 aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac
      361 tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt
      421 atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga
      481 atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag
      541 cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac
      601 cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat
      661 aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata
      721 caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt
      781 cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac
      841 cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct
      901 gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt
      961 gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa
     1021 aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag
     1081 aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt
     1141 atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca
     1201 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
     1261 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
     1321 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
     1381 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
     1441 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
     1501 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
     1561 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
     1621 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
     1681 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
     1741 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
     1801 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
     1861 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
     1921 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
     1981 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
     2041 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
     2101 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
     2161 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttggct ctttgtcaaa
     2221 tgatgttggt aaagagaaat ct
//
LOCUS       DACEKQ010000269         1467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E306
            SAMN07141408-rid9870633.guided.217, whole genome shotgun sequence.
ACCESSION   DACEKQ010000269 DACEKQ010000000
VERSION     DACEKQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141408
            Sequence Read Archive: SRR5811526
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740856.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 02:20:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,080
            CDSs (total)                      :: 3,041
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 31 of 73
            Pseudo Genes (incomplete)         :: 36 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 12 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141408-rid9870633.guided.217"
                     /isolate="E306"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2013"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1348
                     /locus_tag="IW671_003080"
     CDS             <1..1348
                     /locus_tag="IW671_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0899991.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          246 g          482 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgtt
//
